Lag0034237 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0034237
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Glycine--tRNA ligase
Location	chr3: 5553867 .. 5554454 (+)
RNA-Seq Expression	Lag0034237
Synteny	Lag0034237

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATTCCTTTATCCAATGGACAATTCGAATTGTCAAGATTAGGAGACGATGGAGCTCAATCATTGCTGGAAAAATTCTTTATTTCAAAGGAATGGGATTCTCTTTTTGAAAATTCAAGGGCGTCAAGGCAGATACTTGGAGCTTGGGGAATGGGATGGGAAATCTGGATAGATGGGATGGAGATTACCCAATTCACTTACTTTCAGCAGGCTGGAAGTCAACAATTGTTGCCCGTAGTTGTTAAAATCACTTATGGTCTTGAACGTATCCTCATGTTACTGCAGCAAAAGCCAGTATGCTGATGGAAAGGGGAGCTTTTCTTGGAGAACAAGAAGGAAATAAGTGCATATTATTTAGAACATGCCAATGTTCACCAGGTTCAAAAACATTTCAACATCTTTGAGGAGGAACCTCACTCCTTGCTTGCTTTAGGATTAGCGATCCCTGCGTATGATCAGGTTTTGAAGGCATCCCATGCTTTCAACAAATTGGACTCAAGAAGCTTCATTGGGGTATTTCAGAGCATGCTCGTTATTTTGATCAGATGCAAAGCTCTTTTTGAAGATGGTTCCTTTTTTGGTTAA

mRNA sequence

ATGGAAATTCCTTTATCCAATGGACAATTCGAATTGTCAAGATTAGGAGACGATGGAGCTCAATCATTGCTGGAAAAATTCTTTATTTCAAAGGAATGGGATTCTCTTTTTGAAAATTCAAGGGCGTCAAGGCAGATACTTGGAGCTTGGGGAATGGGATGGGAAATCTGGATAGATGGGATGGAGATTACCCAATTCACTTACTTTCAGCAGGCTGGAAGTCAACAATTGTTGCCCGTAGTTGTTAAAATCACTTATGGTCTTGAACGTATCCTCATGTTACTGCAGCAAAAGCCAGTTCAAAAACATTTCAACATCTTTGAGGAGGAACCTCACTCCTTGCTTGCTTTAGGATTAGCGATCCCTGCGTATGATCAGGTTTTGAAGGCATCCCATGCTTTCAACAAATTGGACTCAAGAAGCTTCATTGGGGTATTTCAGAGCATGCTCGTTATTTTGATCAGATGCAAAGCTCTTTTTGAAGATGGTTCCTTTTTTGGTTAA

Coding sequence (CDS)

ATGGAAATTCCTTTATCCAATGGACAATTCGAATTGTCAAGATTAGGAGACGATGGAGCTCAATCATTGCTGGAAAAATTCTTTATTTCAAAGGAATGGGATTCTCTTTTTGAAAATTCAAGGGCGTCAAGGCAGATACTTGGAGCTTGGGGAATGGGATGGGAAATCTGGATAGATGGGATGGAGATTACCCAATTCACTTACTTTCAGCAGGCTGGAAGTCAACAATTGTTGCCCGTAGTTGTTAAAATCACTTATGGTCTTGAACGTATCCTCATGTTACTGCAGCAAAAGCCAGTTCAAAAACATTTCAACATCTTTGAGGAGGAACCTCACTCCTTGCTTGCTTTAGGATTAGCGATCCCTGCGTATGATCAGGTTTTGAAGGCATCCCATGCTTTCAACAAATTGGACTCAAGAAGCTTCATTGGGGTATTTCAGAGCATGCTCGTTATTTTGATCAGATGCAAAGCTCTTTTTGAAGATGGTTCCTTTTTTGGTTAA

Protein sequence

MEIPLSNGQFELSRLGDDGAQSLLEKFFISKEWDSLFENSRASRQILGAWGMGWEIWIDGMEITQFTYFQQAGSQQLLPVVVKITYGLERILMLLQQKPVQKHFNIFEEEPHSLLALGLAIPAYDQVLKASHAFNKLDSRSFIGVFQSMLVILIRCKALFEDGSFFG

Homology

[Close]

BLAST of Lag0034237 vs. NCBI nr
Match: XP_004140508.3 (glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumis sativus] >KAE8646780.1 hypothetical protein Csa_005464 [Cucumis sativus])

HSP 1 Score: 168.3 bits (425), Expect = 5.6e-38
Identity = 98/170 (57.65%), Postives = 105/170 (61.76%), Query Frame = 0


Query: 12  LSRLGDDGAQSLLEKFFISKEWDSLFENSRASRQILGAWGMGWEIWIDGMEITQFTYFQQ 71
           LS LG D A   +   F+   W+S          +LGAWG+GWEIW+DGMEITQFTYFQQ
Sbjct: 167 LSALGIDVAAHDIR--FVEDNWES---------PVLGAWGLGWEIWMDGMEITQFTYFQQ 226

Query: 72  AGSQQLLPVVVKITYGLERILMLLQ----------------------------------- 131
           AGSQQLLPV V+ITYGLERILMLLQ                                   
Sbjct: 227 AGSQQLLPVSVEITYGLERILMLLQGVNHFKKIQYADGITYGELFLENEKEMSAYYLEHA 286

Query: 132 -QKPVQKHFNIFEEEPHSLLALGLAIPAYDQVLKASHAFNKLDSRSFIGV 146
               VQKHFNIFEEE HSLLALGLAIPAYDQVLKASHAFN LDSR FIGV
Sbjct: 287 NVHQVQKHFNIFEEEAHSLLALGLAIPAYDQVLKASHAFNILDSRGFIGV 325

[Close]

BLAST of Lag0034237 vs. NCBI nr
Match: XP_022156971.1 (glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Momordica charantia])

HSP 1 Score: 167.9 bits (424), Expect = 7.3e-38
Identity = 97/170 (57.06%), Postives = 105/170 (61.76%), Query Frame = 0


Query: 12  LSRLGDDGAQSLLEKFFISKEWDSLFENSRASRQILGAWGMGWEIWIDGMEITQFTYFQQ 71
           LS LG D A   +   F+   W+S          +LGAWG+GWEIW+DGMEITQFTYFQQ
Sbjct: 169 LSALGIDVAAHDIR--FVEDNWES---------PVLGAWGLGWEIWMDGMEITQFTYFQQ 228

Query: 72  AGSQQLLPVVVKITYGLERILMLLQ----------------------------------- 131
           AGSQQLLP+ V+ITYGLERILMLLQ                                   
Sbjct: 229 AGSQQLLPISVEITYGLERILMLLQGVNHFKKIQYADGITYGELFLENEKEMSAYYLEHA 288

Query: 132 -QKPVQKHFNIFEEEPHSLLALGLAIPAYDQVLKASHAFNKLDSRSFIGV 146
               VQKHFNIFEEE HSLLALGLAIPAYDQVLKASHAFN LDSR FIGV
Sbjct: 289 NVHQVQKHFNIFEEEAHSLLALGLAIPAYDQVLKASHAFNILDSRGFIGV 327

[Close]

BLAST of Lag0034237 vs. NCBI nr
Match: XP_038874261.1 (glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 166.4 bits (420), Expect = 2.1e-37
Identity = 97/170 (57.06%), Postives = 105/170 (61.76%), Query Frame = 0


Query: 12  LSRLGDDGAQSLLEKFFISKEWDSLFENSRASRQILGAWGMGWEIWIDGMEITQFTYFQQ 71
           LS LG D A   +   F+   W+S          +LGAWG+GWEIW+DGMEITQFTYFQQ
Sbjct: 167 LSALGIDVAAHDIR--FVEDNWES---------PVLGAWGLGWEIWMDGMEITQFTYFQQ 226

Query: 72  AGSQQLLPVVVKITYGLERILMLLQ----------------------------------- 131
           AGSQQLLPV V+ITYGLERILMLLQ                                   
Sbjct: 227 AGSQQLLPVSVEITYGLERILMLLQGVNHFKKIQYADGITYGELFLENEKEMSAYYLEHA 286

Query: 132 -QKPVQKHFNIFEEEPHSLLALGLAIPAYDQVLKASHAFNKLDSRSFIGV 146
               VQKHF+IFEEE HSLLALGLAIPAYDQVLKASHAFN LDSR FIGV
Sbjct: 287 NVHQVQKHFDIFEEEAHSLLALGLAIPAYDQVLKASHAFNILDSRGFIGV 325

[Close]

BLAST of Lag0034237 vs. NCBI nr
Match: XP_008459797.1 (PREDICTED: glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumis melo])

HSP 1 Score: 166.4 bits (420), Expect = 2.1e-37
Identity = 97/170 (57.06%), Postives = 105/170 (61.76%), Query Frame = 0


Query: 12  LSRLGDDGAQSLLEKFFISKEWDSLFENSRASRQILGAWGMGWEIWIDGMEITQFTYFQQ 71
           LS LG D A   +   F+   W+S          +LGAWG+GWEIW+DGMEITQFTYFQQ
Sbjct: 167 LSALGIDVAAHDIR--FVEDNWES---------PVLGAWGLGWEIWMDGMEITQFTYFQQ 226

Query: 72  AGSQQLLPVVVKITYGLERILMLLQ----------------------------------- 131
           AGSQQLLPV V+ITYGLERILMLLQ                                   
Sbjct: 227 AGSQQLLPVSVEITYGLERILMLLQGVNHFKKIQYADGITYGELFLENEKEMSAYYLEHA 286

Query: 132 -QKPVQKHFNIFEEEPHSLLALGLAIPAYDQVLKASHAFNKLDSRSFIGV 146
               VQKHF+IFEEE HSLLALGLAIPAYDQVLKASHAFN LDSR FIGV
Sbjct: 287 NVHQVQKHFDIFEEEAHSLLALGLAIPAYDQVLKASHAFNILDSRGFIGV 325

[Close]

BLAST of Lag0034237 vs. NCBI nr
Match: XP_016902503.1 (PREDICTED: glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucumis melo])

HSP 1 Score: 166.4 bits (420), Expect = 2.1e-37
Identity = 97/170 (57.06%), Postives = 105/170 (61.76%), Query Frame = 0


Query: 12  LSRLGDDGAQSLLEKFFISKEWDSLFENSRASRQILGAWGMGWEIWIDGMEITQFTYFQQ 71
           LS LG D A   +   F+   W+S          +LGAWG+GWEIW+DGMEITQFTYFQQ
Sbjct: 63  LSALGIDVAAHDIR--FVEDNWES---------PVLGAWGLGWEIWMDGMEITQFTYFQQ 122

Query: 72  AGSQQLLPVVVKITYGLERILMLLQ----------------------------------- 131
           AGSQQLLPV V+ITYGLERILMLLQ                                   
Sbjct: 123 AGSQQLLPVSVEITYGLERILMLLQGVNHFKKIQYADGITYGELFLENEKEMSAYYLEHA 182

Query: 132 -QKPVQKHFNIFEEEPHSLLALGLAIPAYDQVLKASHAFNKLDSRSFIGV 146
               VQKHF+IFEEE HSLLALGLAIPAYDQVLKASHAFN LDSR FIGV
Sbjct: 183 NVHQVQKHFDIFEEEAHSLLALGLAIPAYDQVLKASHAFNILDSRGFIGV 221

[Close]

BLAST of Lag0034237 vs. ExPASy Swiss-Prot
Match: Q8L785 (Glycine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EDD1 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.1e-33
Identity = 79/154 (51.30%), Postives = 92/154 (59.74%), Query Frame = 0


Query: 28  FISKEWDSLFENSRASRQILGAWGMGWEIWIDGMEITQFTYFQQAGSQQLLPVVVKITYG 87
           F+   W+S          +LGAWG+GWEIW+DGMEITQFTYFQQAGS  L PV V+ITYG
Sbjct: 180 FVEDNWES---------PVLGAWGLGWEIWMDGMEITQFTYFQQAGSLPLSPVSVEITYG 239

Query: 88  LERILMLLQQ------------------------------------KPVQKHFNIFEEEP 146
           LERI+MLLQ+                                      +QKHF+ F+EE 
Sbjct: 240 LERIIMLLQEVDHFKKILYADGITYGELFLENEKEMSSYYLEHASVDRLQKHFDYFDEEA 299

[Close]

BLAST of Lag0034237 vs. ExPASy Swiss-Prot
Match: Q8RH45 (Glycine--tRNA ligase alpha subunit OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=glyQ PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 5.6e-25
Identity = 67/162 (41.36%), Postives = 83/162 (51.23%), Query Frame = 0


Query: 28  FISKEWDSLFENSRASRQILGAWGMGWEIWIDGMEITQFTYFQQAGSQQLLPVVVKITYG 87
           F+  +W+S           LGAWG+GWE+W+DGMEITQFTYFQQ G  +L  + V+ITYG
Sbjct: 110 FVEDDWES---------PTLGAWGLGWEVWLDGMEITQFTYFQQVGGLELEVIPVEITYG 169

Query: 88  LERILMLLQQK---------------------------------PVQKHFNIF---EEEP 147
           LER+ + +Q K                                  + KHF  F   EEE 
Sbjct: 170 LERLALYIQNKENVYDLEWTKGVKYGDMRYQFEFENSKYSFELATLDKHFKWFDEYEEEA 229

Query: 148 HSLLALGLAIPAYDQVLKASHAFNKLDSRSFIGVFQSMLVIL 154
             +L  GL +PAYD VLK SHAFN LDSR  I   + M  IL
Sbjct: 230 KKILDQGLVLPAYDYVLKCSHAFNVLDSRGAISTTERMGYIL 262

[Close]

BLAST of Lag0034237 vs. ExPASy Swiss-Prot
Match: A2C3W9 (Glycine--tRNA ligase alpha subunit OS=Prochlorococcus marinus (strain NATL1A) OX=167555 GN=glyQ PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-24
Identity = 70/176 (39.77%), Postives = 86/176 (48.86%), Query Frame = 0


Query: 18  DGAQ----SLLEKFFIS-KEWDSLFENSRASRQILGAWGMGWEIWIDGMEITQFTYFQQA 77
           DG Q    S LE   IS KE D  F         LGAWG+GWE+W+DGME+TQFTYFQQ 
Sbjct: 86  DGIQEIYLSSLEALGISAKEHDIRFVEDNWESPTLGAWGVGWEVWLDGMEVTQFTYFQQC 145

Query: 78  GSQQLLPVVVKITYGLERILMLLQ------------------------------------ 137
           G     PV ++ITYGLER+ M LQ                                    
Sbjct: 146 GGLDCKPVSIEITYGLERLAMYLQNVESIWDLSWNKKTKYGDIWLPFEKGQCKYNFEESN 205

Query: 138 QKPVQKHFNIFEEEPHSLLALGLAIPAYDQVLKASHAFNKLDSRSFIGVFQSMLVI 153
            + ++K F I+EEE H L+A  L  P  D VLK SH FN L++R  I V +   +I
Sbjct: 206 SENLRKLFEIYEEEAHQLIAKKLPAPCLDYVLKCSHTFNLLEARGVISVTERTKII 261

[Close]

BLAST of Lag0034237 vs. ExPASy Swiss-Prot
Match: B5YJ13 (Glycine--tRNA ligase alpha subunit OS=Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87) OX=289376 GN=glyQ PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-24
Identity = 64/154 (41.56%), Postives = 81/154 (52.60%), Query Frame = 0


Query: 28  FISKEWDSLFENSRASRQILGAWGMGWEIWIDGMEITQFTYFQQAGSQQLLPVVVKITYG 87
           F+  +W+S           LGAWG+GWE+W+DGMEITQFTYFQQ G   L PV V+ITYG
Sbjct: 110 FVEDDWES---------PTLGAWGLGWEVWLDGMEITQFTYFQQVGGIDLKPVSVEITYG 169

Query: 88  LERILMLLQQ--------------------KP----------------VQKHFNIFEEEP 146
           LERI M LQ+                    +P                ++KHF+ +E E 
Sbjct: 170 LERIAMYLQEVENVFDIKWNEKFTYGDIHKQPEIEFSYFNFDHSDPVIIKKHFDEYERES 229

[Close]

BLAST of Lag0034237 vs. ExPASy Swiss-Prot
Match: C4XS53 (Glycine--tRNA ligase alpha subunit OS=Desulfovibrio magneticus (strain ATCC 700980 / DSM 13731 / RS-1) OX=573370 GN=glyQ PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.1e-24
Identity = 65/154 (42.21%), Postives = 77/154 (50.00%), Query Frame = 0


Query: 28  FISKEWDSLFENSRASRQILGAWGMGWEIWIDGMEITQFTYFQQAGSQQLLPVVVKITYG 87
           F+  +W+S           LGAWG+GWE+W+DGME TQFTYFQQ G   L PV V+ITYG
Sbjct: 110 FVEDDWES---------PTLGAWGLGWEVWLDGMEATQFTYFQQVGGIDLSPVSVEITYG 169

Query: 88  LERILMLLQQKP------------------------------------VQKHFNIFEEEP 146
           LERI M LQQK                                     +  HFN  E+E 
Sbjct: 170 LERITMYLQQKDSVYDLDWNDQVTYGQVHQRGEYEHSRYNFEESDAAMLLAHFNACEKEC 229

The following BLAST results are available for this feature:

BLAST of Lag0034237 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140508.3	5.6e-38	57.65	glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumis sativus]...	[more]
XP_022156971.1	7.3e-38	57.06	glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Momordica charan...	[more]
XP_038874261.1	2.1e-37	57.06	glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Benincasa hispid...	[more]
XP_008459797.1	2.1e-37	57.06	PREDICTED: glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucum...	[more]
XP_016902503.1	2.1e-37	57.06	PREDICTED: glycine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucum...	[more]

BLAST of Lag0034237 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L785	1.1e-33	51.30	Glycine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3...	[more]
Q8RH45	5.6e-25	41.36	Glycine--tRNA ligase alpha subunit OS=Fusobacterium nucleatum subsp. nucleatum (...	[more]
A2C3W9	1.3e-24	39.77	Glycine--tRNA ligase alpha subunit OS=Prochlorococcus marinus (strain NATL1A) OX...	[more]
B5YJ13	1.3e-24	41.56	Glycine--tRNA ligase alpha subunit OS=Thermodesulfovibrio yellowstonii (strain A...	[more]
C4XS53	2.1e-24	42.21	Glycine--tRNA ligase alpha subunit OS=Desulfovibrio magneticus (strain ATCC 7009...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002310	Glycine-tRNA ligase, alpha subunit	PRINTS	PR01044	TRNASYNTHGA	coord: 60..80 score: 68.4 coord: 34..58 score: 42.55 coord: 83..98 score: 61.36
IPR002310	Glycine-tRNA ligase, alpha subunit	PFAM	PF02091	tRNA-synt_2e	coord: 37..98 e-value: 3.1E-25 score: 89.0 coord: 100..152 e-value: 6.0E-10 score: 38.8
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 16..125 e-value: 9.6E-27 score: 95.5
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 46..152
IPR006194	Glycine-tRNA synthetase, heterodimeric	PROSITE	PS50861	AA_TRNA_LIGASE_II_GLYAB	coord: 47..106 score: 17.713488

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0034237.1	Lag0034237.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006426	glycyl-tRNA aminoacylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004820	glycine-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding