Lag0034226 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0034226 vs. NCBI nr
Match: XP_038878610.1 (polygalacturonase 1 beta-like protein 3 [Benincasa hispida])

HSP 1 Score: 938.7 bits (2425), Expect = 2.4e-269
Identity = 497/628 (79.14%), Postives = 523/628 (83.28%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLS FHVSFASAD        Q +PFTAKASL RYWNSHVSN LP P
Sbjct: 1   MVHIVFFLLLSQLSLSFFHVSFASADHQ------QSNPFTAKASLLRYWNSHVSNQLPPP 60

Query: 61  DFLFSKASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLFSKASPLSAVDAAVLA HAA GSL  R ESFCY ANLFCSFDSETEISVPR KDADF
Sbjct: 61  DFLFSKASPLSAVDAAVLAQHAARGSLSFRLESFCYSANLFCSFDSETEISVPRLKDADF 120

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NFVNYG ARLGG DSFKNYSDGLN+A++SFKRYGGNSSNH EGFTNYAEDGNVA
Sbjct: 121 KLYGNKNFVNYGGARLGGTDSFKNYSDGLNTADHSFKRYGGNSSNHEEGFTNYAEDGNVA 180

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGSQSF 240
            SNF+SYA ESKGGSGEF NYDKLVNVPNLRFTAYDL+S+ H LSFT YGNETNSGSQ+F
Sbjct: 181 NSNFISYATESKGGSGEFVNYDKLVNVPNLRFTAYDLNSRNHKLSFTGYGNETNSGSQTF 240

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGK GNGVPQEF SYS+ SNILTSTFTGYGESGKTANDSFK YG+S NNPHTNFKNYG
Sbjct: 241 TSYGKKGNGVPQEFSSYSDGSNILTSTFTGYGESGKTANDSFKGYGVSGNNPHTNFKNYG 300

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GGNS+IDTFSNYRNGANVGDDSFQSYAK SNSGRVNFANYGKSFNHGND+F EYG+GSI
Sbjct: 301 KGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANYGKSFNHGNDSFTEYGKGSI 360

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINRQNATE----IIPSQAHSG 420
           G+TTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGI+     E       S+   G
Sbjct: 361 GRTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIHVNKLVEPGKFFRESELKQG 420

Query: 421 KFTLLLVEPQRDK----ADLPRARWFD--------------DGARDNQRASRVRAGA--- 480
              ++++   RDK    + LPR    +                ARD     RV   A   
Sbjct: 421 --NVMVMPDIRDKMPPRSFLPRPILVNLPFSSSRISEAKEIFHARDGSTMERVITNALGE 480

Query: 481 -----EPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGEL 540
                 PGETKRCVGSAEDMIDFAT VLGR VVARTTANVNGS EKVEIGTVKGINGG+L
Sbjct: 481 CERAPSPGETKRCVGSAEDMIDFATTVLGRRVVARTTANVNGSREKVEIGTVKGINGGDL 540

Query: 541 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGA 599
           T SVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKTKEKINRGVAICHMDTSSWGPEHGA
Sbjct: 541 TSSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTKEKINRGVAICHMDTSSWGPEHGA 600

BLAST of Lag0034226 vs. NCBI nr
Match: KAG6589452.1 (Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023135.1 Polygalacturonase 1 beta-like protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 929.1 bits (2400), Expect = 1.9e-266
Identity = 490/628 (78.03%), Postives = 529/628 (84.24%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD  T NASIQF+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIQFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF+KASPLSAVDAAV A HAA GSL SR E+FCY ANLFCSFDS+ E S+PR KDADF
Sbjct: 66  DFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSIPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+A++SFKRYGGNSSNH+EGF NYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFMNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDS+ H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTFTGYGESGK+ANDSFKAYGLS NNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSGNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEYG+GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYGKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINRQNATE----IIPSQAHSG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGIN     E       S+   G
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 KFTLLLVEPQRDK----ADLPRA-----RWFDDGARDNQRASRVRAGA------------ 480
              ++++   RDK    + LPRA      +      + ++    R G+            
Sbjct: 426 --NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMEHVITSALAE 485

Query: 481 -----EPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGEL 540
                  GETKRCVGSAEDMIDFATAVLGRNVVARTTA+VNGSTEKVEIG VKGINGGEL
Sbjct: 486 CERAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASVNGSTEKVEIGEVKGINGGEL 545

Query: 541 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGA 599
           TRSVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGPEHGA
Sbjct: 546 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGA 605

BLAST of Lag0034226 vs. NCBI nr
Match: XP_022921994.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita moschata])

HSP 1 Score: 927.9 bits (2397), Expect = 4.3e-266
Identity = 490/628 (78.03%), Postives = 528/628 (84.08%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD  T NASIQF+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIQFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF+KASPLSAVDAAV A HAA GSL SR E+FCY ANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+A++SFKRYGGNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFTNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDS+ H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTFTGYGESGK+ANDSFKAYGLS NNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSGNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEYG+GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYGKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINRQNATE----IIPSQAHSG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGIN     E       S+   G
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 KFTLLLVEPQRDK----ADLPRA-----RWFDDGARDNQRASRVRAGA------------ 480
              ++++   RDK    + LPRA      +      + ++    R G+            
Sbjct: 426 --NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMEHVITSALAE 485

Query: 481 -----EPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGEL 540
                  GETKRCVGSAEDMIDFATAVLGRNVV RTTA+VNGS EKVEIG VKGINGGEL
Sbjct: 486 CERAPSMGETKRCVGSAEDMIDFATAVLGRNVVVRTTASVNGSKEKVEIGEVKGINGGEL 545

Query: 541 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGA 599
           TRSVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGPEHGA
Sbjct: 546 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGA 605

BLAST of Lag0034226 vs. NCBI nr
Match: XP_022988353.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita maxima])

HSP 1 Score: 927.9 bits (2397), Expect = 4.3e-266
Identity = 493/628 (78.50%), Postives = 528/628 (84.08%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD  T NASI F+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIHFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF KASPLSAVDAAV A HAA GSL SR E+FCY ANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFVKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+A++SFKRYGGNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFTNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDS+ H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTFTGYGESGK+ANDSFKAYGLSSNNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSSNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEY +GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYAKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINRQNATE----IIPSQAHSG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGIN     E       S+   G
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 KFTLLLVEPQRDK----ADLPRARWFD--------------DGARDNQRASRVRAGA--- 480
              ++++   RDK    + LPRA   +                AR+     RV   A   
Sbjct: 426 --NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMERVITSALAE 485

Query: 481 -----EPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGEL 540
                  GETKRCVGSAEDMIDFATAVLGRNVVARTTA+VNGSTEKVEIG VKGINGGEL
Sbjct: 486 CERAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASVNGSTEKVEIGEVKGINGGEL 545

Query: 541 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGA 599
           TRSVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGPEHGA
Sbjct: 546 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGA 605

BLAST of Lag0034226 vs. NCBI nr
Match: XP_023516985.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 924.9 bits (2389), Expect = 3.6e-265
Identity = 488/628 (77.71%), Postives = 528/628 (84.08%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD  T NASIQF+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIQFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF+KASPLS VDAAV A HAA GSL SR E+FCY ANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFAKASPLSVVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+A++SFKRY GNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYSGNSSNHKEGFTNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDS+ H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTF+GYGESGK+ANDSFKAYGLS NNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFSGYGESGKSANDSFKAYGLSGNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEYG+GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYGKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINRQNATE----IIPSQAHSG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGIN     E       S+   G
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 KFTLLLVEPQRDK----ADLPRA-----RWFDDGARDNQRASRVRAGA------------ 480
              ++++   RDK    + LPRA      +      + ++    R G+            
Sbjct: 426 --NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMEHVITSTLAE 485

Query: 481 -----EPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGEL 540
                  GETKRCVGSAEDMIDFATAVLGRNVVARTTA+VNGSTEKVEIG VKGINGGEL
Sbjct: 486 CERAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASVNGSTEKVEIGEVKGINGGEL 545

Query: 541 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGA 599
           TRSVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGP+HGA
Sbjct: 546 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPKHGA 605

BLAST of Lag0034226 vs. ExPASy Swiss-Prot
Match: P92990 (Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 PE=2 SV=2)

HSP 1 Score: 575.9 bits (1483), Expect = 5.4e-163
Identity = 322/624 (51.60%), Postives = 401/624 (64.26%), Query Frame = 0

Query: 7   FLLLSHLSLSLFHVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSK 66
           FLLL   S  LF+V            S + +PFT KASL RYWN  +    P+ +FL SK
Sbjct: 5   FLLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISK 64

Query: 67  ASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNR 126
           ASPL+AVD+A  +  AA  SL +RF  FC  ANLFC  D    +      D  F +Y  +
Sbjct: 65  ASPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLE-KHDDDVKFSVYDQK 124

Query: 127 NFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVATSNFVS 186
           NF NYG AR GG DSFKNYS   N   +SF+RY  N++ H + FT Y E+ NV    F S
Sbjct: 125 NFTNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNS 184

Query: 187 YAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGS-QSFTSYGK 246
           Y     GG+G+FTNY   VN P  RFTAY     G + +F +Y +E N+G+ QSFTSYGK
Sbjct: 185 YGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGK 244

Query: 247 NGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNS 306
           NGNGVP EF SY  SSN++ S F+ YGESG  AND+F +YG   N P  NF NYG  GN+
Sbjct: 245 NGNGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNA 304

Query: 307 QIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTV 366
            +DTF+NYR+ ANVGDDSF SYAK SNS +VNF NYG+SFN G++TF  YG+G+ G + +
Sbjct: 305 AVDTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEG-SKL 364

Query: 367 GFKSYSVDRNFTTYAQKGVSFSEYSNFTTAT-----GSGINRQNATEIIPSQAHSGKFTL 426
            FK+Y+ +  F  YA+KGV+F++Y+  TT       G  +N+         ++   + T+
Sbjct: 365 SFKTYTPNSTFKDYAKKGVAFAKYNVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTV 424

Query: 427 LLVEPQRDK----ADLPRA----------------RWFDD------GARDNQRASRVRAG 486
           + +   +DK    + LPR+                R F        G       +     
Sbjct: 425 IPMPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERP 484

Query: 487 AEPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGELTRSV 546
              GETKRCVGSAEDMIDFAT+VLGR+VV RTT NV GS EKV IG V GINGG+LT++V
Sbjct: 485 PSVGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAV 544

Query: 547 SCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVAL 599
           SCHQSLYPYLLYYCHSVPKVR+YEAD+L+L +K+KIN G+AICHMDTSSWGP HGAF+AL
Sbjct: 545 SCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLAL 604

BLAST of Lag0034226 vs. ExPASy Swiss-Prot
Match: O80760 (Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 PE=2 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.7e-161
Identity = 310/606 (51.16%), Postives = 388/606 (64.03%), Query Frame = 0

Query: 19  HVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSKASPLSAVDAAVL 78
           +V FA A  T  N +   +PFT KASL RYWN+H++ D PKP F  SKASPL+AVD+   
Sbjct: 20  NVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRF 79

Query: 79  ANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNRNFVNYGAARLGG 138
           A+ A+  +L +    FC  A LFC  +           D +F  Y  +NF NYG+ RL G
Sbjct: 80  ASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTNYGSDRLSG 139

Query: 139 VDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVATSNFVSYAAESKGGSGEF 198
            DSFKNYS G N A +SF+RY  NS+ H +GFTNYA + NVA  +F +YA  + GGSGEF
Sbjct: 140 ADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATGTTGGSGEF 199

Query: 199 TNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFKSYS 258
           TNY+   N PN RFT+Y   + G + +FT+Y    N+G QSFTSY KNGNG P EF  Y 
Sbjct: 200 TNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGAPNEFSGYG 259

Query: 259 ESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNSQIDTFSNYRNGAN 318
             SN++ + FT YGES   ANDSF +YG + N P   FK YG+GGN  +  F NYR+ +N
Sbjct: 260 TGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGFKNYRDQSN 319

Query: 319 VGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTVGFKSYSVDRNFTT 378
           +G DSF SYAK SN+ +VNF NYGKSFN G+D F  YG+ ++G   V FK+Y   ++F  
Sbjct: 320 IGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVG-GNVSFKTYGQGQSFKV 379

Query: 379 YAQKGVSFSEYSNFTTATGSGINRQNATEIIPSQAHSGKFTLLLVEPQRDKAD----LPR 438
           Y + GV F+ YSN  ++ G  +N+         +A   + TL+ +   +DK      LPR
Sbjct: 380 YTKDGVVFARYSNNVSSNGKTVNKWVEEGKFFREAMLKEGTLMQMPDIKDKMPKRTFLPR 439

Query: 439 --------------ARWFDDGARDN--------QRASRVRAGAEPGETKRCVGSAEDMID 498
                           W   GA +N           S     A  GETKRCVGSAEDMID
Sbjct: 440 NIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGETKRCVGSAEDMID 499

Query: 499 FATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGELTRSVSCHQSLYPYLLYYCHSVP 558
           FAT+VLGR VV RTT NV GS +KV IG V GINGG++TR+VSCHQSLYPYLLYYCHSVP
Sbjct: 500 FATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSLYPYLLYYCHSVP 559

Query: 559 KVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGPGRIEVCHWIFENDM 599
           +VR+YE D+L  K+ EKIN GVAICH+DTS+W P HGAF+ALGSGPG+IEVCHWIFENDM
Sbjct: 560 RVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQIEVCHWIFENDM 619

BLAST of Lag0034226 vs. ExPASy Swiss-Prot
Match: P92982 (Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 PE=2 SV=2)

HSP 1 Score: 543.1 bits (1398), Expect = 3.9e-153
Identity = 308/621 (49.60%), Postives = 388/621 (62.48%), Query Frame = 0

Query: 6   FFLLLSHLSLSLFHVSFASADATTLNA-SIQFSPFTAKASLARYWNSHVSNDLPKPDFLF 65
           F  LL  LS  L+HV  A  +  T    S + +PFT KASL RYWN  + +  P   FL 
Sbjct: 5   FVFLLPFLS-RLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLL 64

Query: 66  SKASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYG 125
           SKASPL+AV +A  A  A+  +L  R   FC  ANLFC  D    +    S D  F +Y 
Sbjct: 65  SKASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLE-KHSNDVKFSVYD 124

Query: 126 NRNFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVATSNF 185
            RNF NYG    GG DSFK YS   N+  +SF+RY  +++ H + FT YA++ NV    F
Sbjct: 125 QRNFTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKF 184

Query: 186 VSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSG-SQSFTSY 245
            SY   + GGSG+F NY   VN P  RF AY  +  G   +F +Y +E NSG  QSFTSY
Sbjct: 185 NSYGTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSY 244

Query: 246 GKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGG 305
           GKNGNG P EF SY  SSN++ S F+ YGE+G  AND+FK YG   N P  NFK+YG  G
Sbjct: 245 GKNGNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASG 304

Query: 306 NSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKT 365
           N+ +DTF NYR+ ANVGDDSF SYAK SN  +V+F NYGKS N G+++F  YG+G+ G  
Sbjct: 305 NAAVDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEG-N 364

Query: 366 TVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINRQNATEIIPSQAHSGKFTLLLV 425
            + FK+Y+ +  F  Y + GV F++Y+  +   G  +N+         ++   + TL+ +
Sbjct: 365 KIDFKTYTQNSTFKDYTKTGVEFAKYNRSSLGGGKTVNKWVEPGKFFRESMLKEGTLIWM 424

Query: 426 EPQRDK----ADLPRA----------------RWF---DDGARDNQRASRVRAGAEP--- 485
              +DK    + LPR+                R F   D+   +      VR    P   
Sbjct: 425 PDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPPTV 484

Query: 486 GETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGELTRSVSCH 545
            ETKRCVGSAEDMIDFAT+VLGR+VV RTT +V GS EKV IG V GINGG +T+SVSCH
Sbjct: 485 SETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVSCH 544

Query: 546 QSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSG 599
           QSLYPYLLYYCHSVPKVR+YE+D+L  K+K KIN G+AICHMDTS+WG  HGAF+ LGS 
Sbjct: 545 QSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLGSR 604

BLAST of Lag0034226 vs. ExPASy Swiss-Prot
Match: P93217 (Polygalacturonase non-catalytic subunit AroGP2 OS=Solanum lycopersicum OX=4081 GN=GP2 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 7.6e-149
Identity = 304/631 (48.18%), Postives = 387/631 (61.33%), Query Frame = 0

Query: 6   FFLLLSHLSLSLFHVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFS 65
           + LL+   SLS F++  A     + N      PFT K  + RYWN HVSNDLPKP FL +
Sbjct: 10  YILLVLLFSLSSFNIVVAKDGDESGN------PFTPKGYVIRYWNKHVSNDLPKPWFLLN 69

Query: 66  KASPLSAVDAAVLANHAAG-GSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYG 125
           KASPL+A   A      A   +L +  +SFC  ANL C+ D    +    + D  F  YG
Sbjct: 70  KASPLNAAQYATYTKLVADQNALSTHLQSFCSSANLMCAPDLLPSLE-KHTGDIHFTTYG 129

Query: 126 NRNFVNYGAARLG-GVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVATSN 185
           N+NF NYG    G GV++FKNYS+  +++ NSF+RYG  S    + F NYA DGNV   +
Sbjct: 130 NKNFTNYGTNEPGIGVNTFKNYSE--DASVNSFRRYGRGSPRDNK-FDNYAPDGNVIDQS 189

Query: 186 FVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGSQSFTSY 245
           F SY+  + GGSG+FTNY    NVP+LRFTAY     G    F +Y  + NSG QSF SY
Sbjct: 190 FNSYSTNTPGGSGQFTNYAPNTNVPDLRFTAYSDQGTGGEQEFKTYLEQGNSGGQSFKSY 249

Query: 246 GKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGG 305
           GKNGNG   +F SY   +N+  STF  YG++    N +F +Y  + NNP  NFKNYG GG
Sbjct: 250 GKNGNGADSKFTSYGNETNVAASTFKNYGQNANGENQNFTSYSTNGNNPQNNFKNYGVGG 309

Query: 306 NSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKT 365
           N   +TF+NYR+ +NVGDD F +Y K +N+G  NF NYG+SFN G D F  YG+G     
Sbjct: 310 NGPSETFTNYRDESNVGDDKFSNYVKDANAGEANFTNYGQSFNEGTDVFITYGKGG-NDP 369

Query: 366 TVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINRQNATEIIPSQ-AHSGKF---- 425
            + FK+Y V+  F  Y +   +FS Y N T+   + ++  N  + + ++    GKF    
Sbjct: 370 HINFKTYGVNNTFKDYVKDTATFSNYHNKTSQDLASLSEVNGGKKVNNRWIEPGKFFREK 429

Query: 426 -----TLLLVEPQRDK----ADLPRA---------------RWFDDGARDNQRA------ 485
                T++ +   +DK    + LPRA               +     A D+Q A      
Sbjct: 430 MLKSGTIMPMPDIKDKMPKRSFLPRAIAAKLPFSTSKIDELKKIFHAANDSQVAKMIGDA 489

Query: 486 -SRVRAGAEPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGING 545
            S       PGETK+CV SAEDMIDFAT+VLGRNVV RTT N NGS   + IG++KGING
Sbjct: 490 LSECERAPSPGETKQCVNSAEDMIDFATSVLGRNVVVRTTENTNGSKGNIMIGSIKGING 549

Query: 546 GELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPE 599
           G++T+SVSCHQ+LYP LLYYCHSVPKVR+YEADIL   +K KIN GVAICH+DTSSWGP 
Sbjct: 550 GKVTKSVSCHQTLYPSLLYYCHSVPKVRVYEADILDPNSKAKINHGVAICHVDTSSWGPR 609

BLAST of Lag0034226 vs. ExPASy Swiss-Prot
Match: P93218 (Polygalacturonase non-catalytic subunit AroGP3 OS=Solanum lycopersicum OX=4081 GN=GP3 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 4.6e-146
Identity = 291/601 (48.42%), Postives = 372/601 (61.90%), Query Frame = 0

Query: 37  SPFTAKASLARYWNSHVSNDLPKPDFLFSKASPLSAVDAAVLANHAAGGSLY-SRFESFC 96
           +PFT K  L RYWN HVSNDLPKP FL +KASPL+A   A      A  + + +  +SFC
Sbjct: 35  NPFTPKGYLIRYWNKHVSNDLPKPWFLLNKASPLNAAQYATYTKLVADQNAFTTHLQSFC 94

Query: 97  YLANLFCSFDSETEISVPRSKDADFKLYGNRNFVNYGAARLG-GVDSFKNYSDGLNSAEN 156
             ANL C+ D    +   +  D  F  YG++NF NYG    G G +SFKNY++  N   N
Sbjct: 95  SSANLMCALDLLPSLEKHKG-DVHFVSYGDKNFTNYGTKESGLGFNSFKNYTEEENFPVN 154

Query: 157 SFKRYGGNSSNHREGFTNY-AEDGNVATSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFT 216
           SF+RY G  S H   F NY A  GN    +F SY+  + GGSG+FTNY    NVP+L FT
Sbjct: 155 SFRRY-GRDSPHDNQFDNYGAPGGNTPVQSFNSYSTNTPGGSGQFTNYAPGSNVPDLHFT 214

Query: 217 AYDLDSKGHNLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFKSYSESSNILTSTFTGYGE 276
           +Y     G    F SYGN+ N+G QSF SYGK+GNG   +F +Y  ++N+  S FT YGE
Sbjct: 215 SYSDHGTGGEQDFKSYGNDGNAGQQSFKSYGKDGNGADSQFTTYGNNTNVDGSDFTNYGE 274

Query: 277 SGKTANDSFKAYGLSSNNPHTNFKNYGNGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNS 336
           +    N +F +YG + NNP ++F NYG GGN   + F++YR+ +NVGDD+F +Y K +N 
Sbjct: 275 AANGENQNFTSYGFNGNNPASSFNNYGVGGNGPSEAFNSYRDQSNVGDDTFTTYVKDANG 334

Query: 337 GRVNFANYGKSFNHGNDTFKEYGRGSIGKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFT 396
           G  NF NYG+SFN G D F  YG+G      + FK+Y V+  F  Y +   +FS Y N T
Sbjct: 335 GEANFTNYGQSFNEGTDVFTTYGKGG-NDPHINFKTYGVNNTFKDYVKDTATFSNYHNKT 394

Query: 397 TATGSGINRQNATEIIPSQ-AHSGKF---------TLLLVEPQRDK----ADLPRA---- 456
           +   + ++  N  + + ++    GKF         T++ +   +DK    + LPRA    
Sbjct: 395 SQDLASLSEVNGGKKVNNRWIEPGKFFREKMLKSGTIMPMPDIKDKMPKRSFLPRAIAAK 454

Query: 457 -----------RWFDDGARDNQRA-------SRVRAGAEPGETKRCVGSAEDMIDFATAV 516
                      +     A D+Q A       S       PGETK+CV SAEDMIDFAT+V
Sbjct: 455 LPFSTSKIDELKKIFHAANDSQVAKMIGDALSECERAPSPGETKQCVNSAEDMIDFATSV 514

Query: 517 LGRNVVARTTANVNGSTEKVEIGTVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIY 576
           LGRNVV RTT N NGS   + IG++KGINGG++T+SVSCHQ+LYP LLYYCHSVPKVR+Y
Sbjct: 515 LGRNVVVRTTENTNGSKGNIMIGSIKGINGGKVTKSVSCHQTLYPSLLYYCHSVPKVRVY 574

Query: 577 EADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGPGRIEVCHWIFENDMSWSVA 599
           EADIL   +K KIN GVAICH+DTSSWGP HGAFVALGSGPG+IEVCHWIFENDM+W+ A
Sbjct: 575 EADILDPNSKAKINHGVAICHVDTSSWGPRHGAFVALGSGPGKIEVCHWIFENDMTWATA 632

BLAST of Lag0034226 vs. ExPASy TrEMBL
Match: A0A6J1JM21 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111485620 PE=4 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 2.1e-266
Identity = 493/628 (78.50%), Postives = 528/628 (84.08%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD  T NASI F+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIHFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF KASPLSAVDAAV A HAA GSL SR E+FCY ANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFVKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+A++SFKRYGGNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFTNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDS+ H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTFTGYGESGK+ANDSFKAYGLSSNNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSSNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEY +GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYAKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINRQNATE----IIPSQAHSG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGIN     E       S+   G
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 KFTLLLVEPQRDK----ADLPRARWFD--------------DGARDNQRASRVRAGA--- 480
              ++++   RDK    + LPRA   +                AR+     RV   A   
Sbjct: 426 --NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMERVITSALAE 485

Query: 481 -----EPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGEL 540
                  GETKRCVGSAEDMIDFATAVLGRNVVARTTA+VNGSTEKVEIG VKGINGGEL
Sbjct: 486 CERAPSMGETKRCVGSAEDMIDFATAVLGRNVVARTTASVNGSTEKVEIGEVKGINGGEL 545

Query: 541 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGA 599
           TRSVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGPEHGA
Sbjct: 546 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGA 605

BLAST of Lag0034226 vs. ExPASy TrEMBL
Match: A0A6J1E242 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111430079 PE=4 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 2.1e-266
Identity = 490/628 (78.03%), Postives = 528/628 (84.08%), Query Frame = 0

Query: 1   MVHIVFFLLLSHLSLSLFHVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKP 60
           MVHIVFFLLLS LSLSL HVSF SAD  T NASIQF+PFTAKASL RYWN+HVSN LPKP
Sbjct: 6   MVHIVFFLLLSQLSLSLLHVSFVSADQATFNASIQFNPFTAKASLVRYWNTHVSNQLPKP 65

Query: 61  DFLFSKASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADF 120
           DFLF+KASPLSAVDAAV A HAA GSL SR E+FCY ANLFCSFDS+ E SVPR KDADF
Sbjct: 66  DFLFAKASPLSAVDAAVFAKHAALGSLTSRLETFCYSANLFCSFDSQ-ETSVPRFKDADF 125

Query: 121 KLYGNRNFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVA 180
           KLYGN+NF NYGAAR+GG DSFKNYSDGLN+A++SFKRYGGNSSNH+EGFTNYAEDGNVA
Sbjct: 126 KLYGNKNFNNYGAARIGGADSFKNYSDGLNTADHSFKRYGGNSSNHKEGFTNYAEDGNVA 185

Query: 181 TSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGSQSF 240
            +NF+SYAAESKGGSGEF NYDK+VNVPNLRFTAYDLDS+ H LSFTSYGNETNSGSQSF
Sbjct: 186 NNNFISYAAESKGGSGEFVNYDKIVNVPNLRFTAYDLDSRAHKLSFTSYGNETNSGSQSF 245

Query: 241 TSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYG 300
           TSYGKNGNGVPQEF SYS+ SNILTSTFTGYGESGK+ANDSFKAYGLS NNPH+NFKNYG
Sbjct: 246 TSYGKNGNGVPQEFSSYSDGSNILTSTFTGYGESGKSANDSFKAYGLSGNNPHSNFKNYG 305

Query: 301 NGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSI 360
            GG SQIDTFSNYRNGANVGDDSFQSYAK SNSGRVNFA+YG+SFNHGND+FKEYG+GSI
Sbjct: 306 KGGKSQIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFASYGQSFNHGNDSFKEYGKGSI 365

Query: 361 GKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINRQNATE----IIPSQAHSG 420
           G+TTVGF+SYSVDRNFTTYAQKGVSFSEYS    ATGSGIN     E       S+   G
Sbjct: 366 GQTTVGFRSYSVDRNFTTYAQKGVSFSEYS----ATGSGINVNRWVEPGKFFRESELKQG 425

Query: 421 KFTLLLVEPQRDK----ADLPRA-----RWFDDGARDNQRASRVRAGA------------ 480
              ++++   RDK    + LPRA      +      + ++    R G+            
Sbjct: 426 --NVMVMPDIRDKMPPRSFLPRAILVNIPFSSSKISETKQIFHARNGSTMEHVITSALAE 485

Query: 481 -----EPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGEL 540
                  GETKRCVGSAEDMIDFATAVLGRNVV RTTA+VNGS EKVEIG VKGINGGEL
Sbjct: 486 CERAPSMGETKRCVGSAEDMIDFATAVLGRNVVVRTTASVNGSKEKVEIGEVKGINGGEL 545

Query: 541 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGA 599
           TRSVSCHQSLYPYLLYYCHSVPKVRIYEADIL+LKT EKINRGVAICHMDTSSWGPEHGA
Sbjct: 546 TRSVSCHQSLYPYLLYYCHSVPKVRIYEADILRLKTNEKINRGVAICHMDTSSWGPEHGA 605

BLAST of Lag0034226 vs. ExPASy TrEMBL
Match: A0A1S3BVD2 (polygalacturonase 1 beta-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103493915 PE=4 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.3e-252
Identity = 471/655 (71.91%), Postives = 513/655 (78.32%), Query Frame = 0

Query: 1   MVHIV----FFLLLSHLSLSLFHVS------------------FASADATTLNASIQFSP 60
           MVHI     FF  +S LSLS FH++                  F  +    ++++   +P
Sbjct: 1   MVHIFSSSSFFFFISQLSLSFFHLTTNKQISFKVLFLFLLPFFFFLSFFPQISSAASSNP 60

Query: 61  FTAKASLARYWNSHVSNDLPKPDFLFSKASPLSAVDAAVLANHAAGGSLYSRFESFCYLA 120
           FT KASL RYW +HVS DLP PDF  SKASPLS+VDAAVLA HAAG SL SR ESFCY A
Sbjct: 61  FTPKASLVRYWKTHVSTDLPTPDFFLSKASPLSSVDAAVLATHAAGASLSSRLESFCYSA 120

Query: 121 NLFCSFDSETEISVPRSKDADFKLYGNRNFVNYGAARLGGVDSFKNYSDGLNSAENSFKR 180
           NLFCSFDS++EISVPR KDADFKLYGN+NF NYG  RLGG DSFKNYSDGLN+AE+SFKR
Sbjct: 121 NLFCSFDSQSEISVPRFKDADFKLYGNKNFANYGGNRLGGTDSFKNYSDGLNTAEHSFKR 180

Query: 181 YGGNSSNHREGFTNYAEDGNVATSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLD 240
           YGGNSSNH+EGFT+YAEDGNVA SNFVSY AESK GSG FTNYDKLVNVPNLRFTAYDL+
Sbjct: 181 YGGNSSNHKEGFTSYAEDGNVANSNFVSYGAESKSGSGGFTNYDKLVNVPNLRFTAYDLN 240

Query: 241 SKGHNLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTA 300
           S+ H L+FTSYGNETNSGSQSFTSYGKNGNGVPQ+F SYS+ SNILTSTFTGYGESGKTA
Sbjct: 241 SRSHKLTFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKTA 300

Query: 301 NDSFKAYGLSSNNPHTNFKNYGNGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNF 360
           NDSFK YG+S NNPHTNFKNYG GGNS+IDTFSNYRNGANVGDDSFQSYAK SNSGRVNF
Sbjct: 301 NDSFKEYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNF 360

Query: 361 ANYGKSFNHGNDTFKEYGRGSIGKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGS 420
           ANYGKSFNHGND+F EYG+GSIGKTTVGFKSYS+DRNFTTYA+ GVSFSEYSNFTTATGS
Sbjct: 361 ANYGKSFNHGNDSFTEYGKGSIGKTTVGFKSYSIDRNFTTYAKNGVSFSEYSNFTTATGS 420

Query: 421 GINRQNATEIIPSQAHSGKF---------TLLLVEPQRDK----ADLPRARWFD------ 480
           GI+       + S    GKF          ++++   RDK    + LPRA   +      
Sbjct: 421 GIH-------VNSHVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAILVNLPFSSS 480

Query: 481 --------DGARDNQRASRVRAGA--------EPGETKRCVGSAEDMIDFATAVLGRNVV 540
                     ARD      V   A          GETKRCVGSAEDMIDFAT VLGR VV
Sbjct: 481 RISEAKEMFHARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGRRVV 540

Query: 541 ARTTANVNGSTEKVEIGTVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILK 599
            RTTANVNGS EKVEIG VKGINGG LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADIL+
Sbjct: 541 VRTTANVNGSKEKVEIGRVKGINGGNLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILR 600

BLAST of Lag0034226 vs. ExPASy TrEMBL
Match: A0A5D3BIQ0 (Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001790 PE=4 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 6.5e-252
Identity = 457/604 (75.66%), Postives = 495/604 (81.95%), Query Frame = 0

Query: 30  LNASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSKASPLSAVDAAVLANHAAGGSLYS 89
           ++++   +PFT KASL RYW +HVS DLP PDF  SKASPLS+VDAAVLA HAAG SL S
Sbjct: 2   ISSAASSNPFTPKASLVRYWKTHVSTDLPTPDFFLSKASPLSSVDAAVLATHAAGASLSS 61

Query: 90  RFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNRNFVNYGAARLGGVDSFKNYSDGL 149
           R ESFCY ANLFCSFDS++EISVPR KDADFKLYGN+NF NYG  RLGG DSFKNYSDGL
Sbjct: 62  RLESFCYSANLFCSFDSQSEISVPRFKDADFKLYGNKNFANYGGNRLGGTDSFKNYSDGL 121

Query: 150 NSAENSFKRYGGNSSNHREGFTNYAEDGNVATSNFVSYAAESKGGSGEFTNYDKLVNVPN 209
           N+AE+SFKRYGGNSSNH+EGFT+YAEDGNVA SNFVSY AESK GSG FTNYDKLVNVPN
Sbjct: 122 NTAEHSFKRYGGNSSNHKEGFTSYAEDGNVANSNFVSYGAESKSGSGGFTNYDKLVNVPN 181

Query: 210 LRFTAYDLDSKGHNLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFKSYSESSNILTSTFT 269
           LRFTAYDL+S+ H L+FTSYGNETNSGSQSFTSYGKNGNGVPQ+F SYS+ SNILTSTFT
Sbjct: 182 LRFTAYDLNSRSHKLTFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFT 241

Query: 270 GYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNSQIDTFSNYRNGANVGDDSFQSYAK 329
           GYGESGKTANDSFK YG+S NNPHTNFKNYG GGNS+IDTFSNYRNGANVGDDSFQSYAK
Sbjct: 242 GYGESGKTANDSFKEYGVSGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAK 301

Query: 330 GSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTVGFKSYSVDRNFTTYAQKGVSFSEY 389
            SNSGRVNFANYGKSFNHGND+F EYG+GSIGKTTVGFKSYS+DRNFTTYA+ GVSFSEY
Sbjct: 302 NSNSGRVNFANYGKSFNHGNDSFTEYGKGSIGKTTVGFKSYSIDRNFTTYAKNGVSFSEY 361

Query: 390 SNFTTATGSGINRQNATEIIPSQAHSGKF---------TLLLVEPQRDK----ADLPRAR 449
           SNFTTATGSGI+       + S    GKF          ++++   RDK    + LPRA 
Sbjct: 362 SNFTTATGSGIH-------VNSHVEPGKFFRESELKQGNVMVMPDIRDKMPPRSFLPRAI 421

Query: 450 WFD--------------DGARDNQRASRVRAGA--------EPGETKRCVGSAEDMIDFA 509
             +                ARD      V   A          GETKRCVGSAEDMIDFA
Sbjct: 422 LVNLPFSSSRISEAKEMFHARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFA 481

Query: 510 TAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKV 569
           T VLGR VV RTTANVNGS EKVEIG VKGINGG LTRSVSCHQSLYPYLLYYCHSVPKV
Sbjct: 482 TTVLGRRVVVRTTANVNGSKEKVEIGRVKGINGGNLTRSVSCHQSLYPYLLYYCHSVPKV 541

Query: 570 RIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGPGRIEVCHWIFENDMSW 599
           RIYEADIL+++TKEKINRGVAICH+DTSSWGPEHGAFVALGS PG+IEVCHWIFENDMSW
Sbjct: 542 RIYEADILRVETKEKINRGVAICHIDTSSWGPEHGAFVALGSSPGQIEVCHWIFENDMSW 598

BLAST of Lag0034226 vs. ExPASy TrEMBL
Match: A0A0A0LP63 (Polygalacturonase-1 non-catalytic subunit beta OS=Cucumis sativus OX=3659 GN=Csa_1G005490 PE=4 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 1.1e-251
Identity = 468/645 (72.56%), Postives = 512/645 (79.38%), Query Frame = 0

Query: 1   MVHIVFFLL--LSHLSLSLFH------VSFASADATTLN-----------ASIQFSPFTA 60
           MVHI  FL+  LS LSLS FH      +SF       L            +S   +PFT 
Sbjct: 1   MVHIFSFLIFFLSQLSLSFFHLTTNKQISFKFLFLFLLPFIFFLSFFPQISSASSNPFTP 60

Query: 61  KASLARYWNSHVSNDLPKPDFLFSKASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLF 120
           KASL RYW +HVS DLP PDF  SKASPLSAVDAA LA HAAG SL SR ESFC+ ANLF
Sbjct: 61  KASLVRYWKTHVSTDLPTPDFFLSKASPLSAVDAAALATHAAGASLSSRLESFCFSANLF 120

Query: 121 CSFDSETEISVPRSKDADFKLYGNRNFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGG 180
           CSFDS++E SVPRSKDADFKLYGN+NFVNYG +RLGG DSFKNYSDGLN+AE+SFKRYGG
Sbjct: 121 CSFDSQSETSVPRSKDADFKLYGNKNFVNYGGSRLGGTDSFKNYSDGLNTAEHSFKRYGG 180

Query: 181 NSSNHREGFTNYAEDGNVATSNFVSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKG 240
           NSSNH+EGFT+YAEDGNVA SNFVSY A SK GSGEFTNYDKLVNVPNLRFTAYDL+S+ 
Sbjct: 181 NSSNHKEGFTSYAEDGNVANSNFVSYGAGSKSGSGEFTNYDKLVNVPNLRFTAYDLNSRS 240

Query: 241 HNLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDS 300
           H LSFTSYGNETNSGSQSFTSYGKNGNGVPQ+F SYS+ SNILTSTFTGYGESGKTANDS
Sbjct: 241 HKLSFTSYGNETNSGSQSFTSYGKNGNGVPQDFSSYSDGSNILTSTFTGYGESGKTANDS 300

Query: 301 FKAYGLSSNNPHTNFKNYGNGGNSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANY 360
           FK YG+S NNPHTNFKNYG GGNS+IDTFSNYRNGANVGDDSFQSYAK SNSGRVNFANY
Sbjct: 301 FKEYGISGNNPHTNFKNYGKGGNSEIDTFSNYRNGANVGDDSFQSYAKNSNSGRVNFANY 360

Query: 361 GKSFNHGNDTFKEYGRGSIGKTTVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGIN 420
           GKSFNHGND+F EYG+GS+GKTTVGFKSYSVDRNFTTYA+ GVSFS Y+NFTTATGSGI+
Sbjct: 361 GKSFNHGNDSFTEYGKGSMGKTTVGFKSYSVDRNFTTYAKNGVSFSVYNNFTTATGSGIH 420

Query: 421 RQNATE----IIPSQAHSGKFTLL--LVEPQRDKADLPRARWFD--------------DG 480
             +  E       S+   G   ++  + +    ++ LPR+   +                
Sbjct: 421 ANSHVEPGKFFRESELKQGNVMVMPDIKDKMPPRSFLPRSILVNLPFSSSRISEAKEMFH 480

Query: 481 ARDNQRASRVRAGA--------EPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGS 540
           ARD      V   A          GETKRCVGSAEDMIDFAT VLG+ VV RTTANVNGS
Sbjct: 481 ARDGSTMESVITNALAECERAPSRGETKRCVGSAEDMIDFATTVLGQRVVVRTTANVNGS 540

Query: 541 TEKVEIGTVKGINGGELTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRG 599
            EK+EIG VKGINGG+LTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILK++TKEKINRG
Sbjct: 541 KEKIEIGRVKGINGGDLTRSVSCHQSLYPYLLYYCHSVPKVRIYEADILKMETKEKINRG 600

BLAST of Lag0034226 vs. TAIR 10
Match: AT1G70370.1 (polygalacturonase 2 )

HSP 1 Score: 575.9 bits (1483), Expect = 3.9e-164
Identity = 322/624 (51.60%), Postives = 401/624 (64.26%), Query Frame = 0

Query: 7   FLLLSHLSLSLFHVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSK 66
           FLLL   S  LF+V            S + +PFT KASL RYWN  +    P+ +FL SK
Sbjct: 5   FLLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISK 64

Query: 67  ASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNR 126
           ASPL+AVD+A  +  AA  SL +RF  FC  ANLFC  D    +      D  F +Y  +
Sbjct: 65  ASPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLE-KHDDDVKFSVYDQK 124

Query: 127 NFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVATSNFVS 186
           NF NYG AR GG DSFKNYS   N   +SF+RY  N++ H + FT Y E+ NV    F S
Sbjct: 125 NFTNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNS 184

Query: 187 YAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGS-QSFTSYGK 246
           Y     GG+G+FTNY   VN P  RFTAY     G + +F +Y +E N+G+ QSFTSYGK
Sbjct: 185 YGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGK 244

Query: 247 NGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNS 306
           NGNGVP EF SY  SSN++ S F+ YGESG  AND+F +YG   N P  NF NYG  GN+
Sbjct: 245 NGNGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNA 304

Query: 307 QIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTV 366
            +DTF+NYR+ ANVGDDSF SYAK SNS +VNF NYG+SFN G++TF  YG+G+ G + +
Sbjct: 305 AVDTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEG-SKL 364

Query: 367 GFKSYSVDRNFTTYAQKGVSFSEYSNFTTAT-----GSGINRQNATEIIPSQAHSGKFTL 426
            FK+Y+ +  F  YA+KGV+F++Y+  TT       G  +N+         ++   + T+
Sbjct: 365 SFKTYTPNSTFKDYAKKGVAFAKYNVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTV 424

Query: 427 LLVEPQRDK----ADLPRA----------------RWFDD------GARDNQRASRVRAG 486
           + +   +DK    + LPR+                R F        G       +     
Sbjct: 425 IPMPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERP 484

Query: 487 AEPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGELTRSV 546
              GETKRCVGSAEDMIDFAT+VLGR+VV RTT NV GS EKV IG V GINGG+LT++V
Sbjct: 485 PSVGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAV 544

Query: 547 SCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVAL 599
           SCHQSLYPYLLYYCHSVPKVR+YEAD+L+L +K+KIN G+AICHMDTSSWGP HGAF+AL
Sbjct: 545 SCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLAL 604

BLAST of Lag0034226 vs. TAIR 10
Match: AT1G70370.2 (polygalacturonase 2 )

HSP 1 Score: 575.9 bits (1483), Expect = 3.9e-164
Identity = 322/624 (51.60%), Postives = 401/624 (64.26%), Query Frame = 0

Query: 7   FLLLSHLSLSLFHVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSK 66
           FLLL   S  LF+V            S + +PFT KASL RYWN  +    P+ +FL SK
Sbjct: 5   FLLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISK 64

Query: 67  ASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNR 126
           ASPL+AVD+A  +  AA  SL +RF  FC  ANLFC  D    +      D  F +Y  +
Sbjct: 65  ASPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLE-KHDDDVKFSVYDQK 124

Query: 127 NFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVATSNFVS 186
           NF NYG AR GG DSFKNYS   N   +SF+RY  N++ H + FT Y E+ NV    F S
Sbjct: 125 NFTNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNS 184

Query: 187 YAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGS-QSFTSYGK 246
           Y     GG+G+FTNY   VN P  RFTAY     G + +F +Y +E N+G+ QSFTSYGK
Sbjct: 185 YGTFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGK 244

Query: 247 NGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNS 306
           NGNGVP EF SY  SSN++ S F+ YGESG  AND+F +YG   N P  NF NYG  GN+
Sbjct: 245 NGNGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNA 304

Query: 307 QIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTV 366
            +DTF+NYR+ ANVGDDSF SYAK SNS +VNF NYG+SFN G++TF  YG+G+ G + +
Sbjct: 305 AVDTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEG-SKL 364

Query: 367 GFKSYSVDRNFTTYAQKGVSFSEYSNFTTAT-----GSGINRQNATEIIPSQAHSGKFTL 426
            FK+Y+ +  F  YA+KGV+F++Y+  TT       G  +N+         ++   + T+
Sbjct: 365 SFKTYTPNSTFKDYAKKGVAFAKYNVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTV 424

Query: 427 LLVEPQRDK----ADLPRA----------------RWFDD------GARDNQRASRVRAG 486
           + +   +DK    + LPR+                R F        G       +     
Sbjct: 425 IPMPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERP 484

Query: 487 AEPGETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGELTRSV 546
              GETKRCVGSAEDMIDFAT+VLGR+VV RTT NV GS EKV IG V GINGG+LT++V
Sbjct: 485 PSVGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAV 544

Query: 547 SCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVAL 599
           SCHQSLYPYLLYYCHSVPKVR+YEAD+L+L +K+KIN G+AICHMDTSSWGP HGAF+AL
Sbjct: 545 SCHQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLAL 604

BLAST of Lag0034226 vs. TAIR 10
Match: AT1G60390.1 (polygalacturonase 1 )

HSP 1 Score: 570.9 bits (1470), Expect = 1.2e-162
Identity = 310/606 (51.16%), Postives = 388/606 (64.03%), Query Frame = 0

Query: 19  HVSFASADATTLNASIQFSPFTAKASLARYWNSHVSNDLPKPDFLFSKASPLSAVDAAVL 78
           +V FA A  T  N +   +PFT KASL RYWN+H++ D PKP F  SKASPL+AVD+   
Sbjct: 20  NVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRF 79

Query: 79  ANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYGNRNFVNYGAARLGG 138
           A+ A+  +L +    FC  A LFC  +           D +F  Y  +NF NYG+ RL G
Sbjct: 80  ASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTNYGSDRLSG 139

Query: 139 VDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVATSNFVSYAAESKGGSGEF 198
            DSFKNYS G N A +SF+RY  NS+ H +GFTNYA + NVA  +F +YA  + GGSGEF
Sbjct: 140 ADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATGTTGGSGEF 199

Query: 199 TNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSGSQSFTSYGKNGNGVPQEFKSYS 258
           TNY+   N PN RFT+Y   + G + +FT+Y    N+G QSFTSY KNGNG P EF  Y 
Sbjct: 200 TNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGAPNEFSGYG 259

Query: 259 ESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGGNSQIDTFSNYRNGAN 318
             SN++ + FT YGES   ANDSF +YG + N P   FK YG+GGN  +  F NYR+ +N
Sbjct: 260 TGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGFKNYRDQSN 319

Query: 319 VGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKTTVGFKSYSVDRNFTT 378
           +G DSF SYAK SN+ +VNF NYGKSFN G+D F  YG+ ++G   V FK+Y   ++F  
Sbjct: 320 IGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVG-GNVSFKTYGQGQSFKV 379

Query: 379 YAQKGVSFSEYSNFTTATGSGINRQNATEIIPSQAHSGKFTLLLVEPQRDKAD----LPR 438
           Y + GV F+ YSN  ++ G  +N+         +A   + TL+ +   +DK      LPR
Sbjct: 380 YTKDGVVFARYSNNVSSNGKTVNKWVEEGKFFREAMLKEGTLMQMPDIKDKMPKRTFLPR 439

Query: 439 --------------ARWFDDGARDN--------QRASRVRAGAEPGETKRCVGSAEDMID 498
                           W   GA +N           S     A  GETKRCVGSAEDMID
Sbjct: 440 NIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGETKRCVGSAEDMID 499

Query: 499 FATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGELTRSVSCHQSLYPYLLYYCHSVP 558
           FAT+VLGR VV RTT NV GS +KV IG V GINGG++TR+VSCHQSLYPYLLYYCHSVP
Sbjct: 500 FATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSLYPYLLYYCHSVP 559

Query: 559 KVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSGPGRIEVCHWIFENDM 599
           +VR+YE D+L  K+ EKIN GVAICH+DTS+W P HGAF+ALGSGPG+IEVCHWIFENDM
Sbjct: 560 RVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQIEVCHWIFENDM 619

BLAST of Lag0034226 vs. TAIR 10
Match: AT1G23760.1 (BURP domain-containing protein )

HSP 1 Score: 543.1 bits (1398), Expect = 2.8e-154
Identity = 308/621 (49.60%), Postives = 388/621 (62.48%), Query Frame = 0

Query: 6   FFLLLSHLSLSLFHVSFASADATTLNA-SIQFSPFTAKASLARYWNSHVSNDLPKPDFLF 65
           F  LL  LS  L+HV  A  +  T    S + +PFT KASL RYWN  + +  P   FL 
Sbjct: 5   FVFLLPFLS-RLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLL 64

Query: 66  SKASPLSAVDAAVLANHAAGGSLYSRFESFCYLANLFCSFDSETEISVPRSKDADFKLYG 125
           SKASPL+AV +A  A  A+  +L  R   FC  ANLFC  D    +    S D  F +Y 
Sbjct: 65  SKASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLE-KHSNDVKFSVYD 124

Query: 126 NRNFVNYGAARLGGVDSFKNYSDGLNSAENSFKRYGGNSSNHREGFTNYAEDGNVATSNF 185
            RNF NYG    GG DSFK YS   N+  +SF+RY  +++ H + FT YA++ NV    F
Sbjct: 125 QRNFTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKF 184

Query: 186 VSYAAESKGGSGEFTNYDKLVNVPNLRFTAYDLDSKGHNLSFTSYGNETNSG-SQSFTSY 245
            SY   + GGSG+F NY   VN P  RF AY  +  G   +F +Y +E NSG  QSFTSY
Sbjct: 185 NSYGTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSY 244

Query: 246 GKNGNGVPQEFKSYSESSNILTSTFTGYGESGKTANDSFKAYGLSSNNPHTNFKNYGNGG 305
           GKNGNG P EF SY  SSN++ S F+ YGE+G  AND+FK YG   N P  NFK+YG  G
Sbjct: 245 GKNGNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASG 304

Query: 306 NSQIDTFSNYRNGANVGDDSFQSYAKGSNSGRVNFANYGKSFNHGNDTFKEYGRGSIGKT 365
           N+ +DTF NYR+ ANVGDDSF SYAK SN  +V+F NYGKS N G+++F  YG+G+ G  
Sbjct: 305 NAAVDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEG-N 364

Query: 366 TVGFKSYSVDRNFTTYAQKGVSFSEYSNFTTATGSGINRQNATEIIPSQAHSGKFTLLLV 425
            + FK+Y+ +  F  Y + GV F++Y+  +   G  +N+         ++   + TL+ +
Sbjct: 365 KIDFKTYTQNSTFKDYTKTGVEFAKYNRSSLGGGKTVNKWVEPGKFFRESMLKEGTLIWM 424

Query: 426 EPQRDK----ADLPRA----------------RWF---DDGARDNQRASRVRAGAEP--- 485
              +DK    + LPR+                R F   D+   +      VR    P   
Sbjct: 425 PDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPPTV 484

Query: 486 GETKRCVGSAEDMIDFATAVLGRNVVARTTANVNGSTEKVEIGTVKGINGGELTRSVSCH 545
            ETKRCVGSAEDMIDFAT+VLGR+VV RTT +V GS EKV IG V GINGG +T+SVSCH
Sbjct: 485 SETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVSCH 544

Query: 546 QSLYPYLLYYCHSVPKVRIYEADILKLKTKEKINRGVAICHMDTSSWGPEHGAFVALGSG 599
           QSLYPYLLYYCHSVPKVR+YE+D+L  K+K KIN G+AICHMDTS+WG  HGAF+ LGS 
Sbjct: 545 QSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLGSR 604

BLAST of Lag0034226 vs. TAIR 10
Match: AT5G25610.1 (BURP domain-containing protein )

HSP 1 Score: 88.6 bits (218), Expect = 1.9e-17
Identity = 47/141 (33.33%), Postives = 73/141 (51.77%), Query Frame = 0

Query: 458 GETKRCVGSAEDMIDFATAVLGR---NVVARTTANVNGSTEKVEIGTVKGINGGELTRSV 517
           GE K C  S E M+DF+ + LG+     V+   A  N   +K +I    G+      +SV
Sbjct: 253 GEEKYCATSLESMVDFSVSKLGKYHVRAVSTEVAKKNAPMQKYKIAAA-GVKKLSDDKSV 312

Query: 518 SCHQSLYPYLLYYCHSVPKVRIYEADILKLKTKEKIN-RGVAICHMDTSSWGPEHGAFVA 577
            CH+  YP+ ++YCH      +Y    + L+ +  +  + VA+CH +TS+W P H AF  
Sbjct: 313 VCHKQKYPFAVFYCHKAMMTTVY---AVPLEGENGMRAKAVAVCHKNTSAWNPNHLAFKV 372

Query: 578 LGSGPGRIEVCHWIFENDMSW 595
           L   PG + VCH++ E  + W
Sbjct: 373 LKVKPGTVPVCHFLPETHVVW 389