Lag0034175 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0034175 vs. NCBI nr
Match: KAG6587515.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 904.4 bits (2336), Expect = 4.4e-259
Identity = 465/522 (89.08%), Postives = 493/522 (94.44%), Query Frame = 0

Query: 1   MEAPSLSNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATA 60
           MEAP  SNRWIAT++SIWIQCICGPSYTFGIYSSALKS+QNY QSTLDTVSVFKDIGATA
Sbjct: 1   MEAPP-SNRWIATVASIWIQCICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATA 60

Query: 61  GLLAGLLYSAVVSHDRPRRPWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFL 120
           G+LAGLLYSAVVS DRP RPWIVL VGAIQCFLGYIF+WAAVSGLIPRP V  MCLFMFL
Sbjct: 61  GVLAGLLYSAVVSTDRPHRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPSVSTMCLFMFL 120

Query: 121 AVHAQVFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVDYLLM 180
           AVHAQVFFNTANVVTGVHNFQLYGGTI+GILKGFLGLSGAVLIQFSNTFY G+P+ YLLM
Sbjct: 121 AVHAQVFFNTANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLM 180

Query: 181 LAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWA 240
           LAILPALTT+LLMRFVVI+KTE GNESTHLNSLS IALIIS YLTILI+LDNV  LPTW 
Sbjct: 181 LAILPALTTVLLMRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFILPTWV 240

Query: 241 RLFTFILLLALLACPLGIAVRAQTEDSVFKTKLQNTDDCVEYHRIPSEDQRNEQLTIVTD 300
           RLFTF+LLL LLA PLGIA RAQTEDSV KTKLQ+TD+ VEYH+IP E+QRN+QL +VT+
Sbjct: 241 RLFTFVLLLILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTN 300

Query: 301 GEMNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWN 360
           GEMN+IEA+GTVNFW+LF AMMCGMGSGLATINNMNQLGQSLGY  VEI+TFVSLWSIWN
Sbjct: 301 GEMNVIEAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKPVEIHTFVSLWSIWN 360

Query: 361 FLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYG 420
           FLGRLGSGYASDLLL +LGWARPLLMA+ALLTMSVGHIIIASGFSGNLYLGSVIVGICYG
Sbjct: 361 FLGRLGSGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYG 420

Query: 421 AQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK 480
           +QWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK
Sbjct: 421 SQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK 480

Query: 481 HCFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLKD 523
           HCFMTSFLIMAAVAFLGFVVAVALF RTRRFYQ+LVQR+LKD
Sbjct: 481 HCFMTSFLIMAAVAFLGFVVAVALFLRTRRFYQLLVQRQLKD 521

BLAST of Lag0034175 vs. NCBI nr
Match: XP_023530984.1 (uncharacterized protein LOC111793373 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 902.9 bits (2332), Expect = 1.3e-258
Identity = 464/522 (88.89%), Postives = 495/522 (94.83%), Query Frame = 0

Query: 1   MEAPSLSNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATA 60
           MEAP  SNRWIAT++SIWIQCICGPSYTFGIYSSALKS+QNY QSTLDTVSVFKDIGA+A
Sbjct: 1   MEAPP-SNRWIATVASIWIQCICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGASA 60

Query: 61  GLLAGLLYSAVVSHDRPRRPWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFL 120
           G+LAGLLYSAVVS DRPRRPWIVL VGAIQCFLGYIF+WAAVSGLIPRPPV AMCLFMFL
Sbjct: 61  GVLAGLLYSAVVSTDRPRRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFL 120

Query: 121 AVHAQVFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVDYLLM 180
           AVHAQVFFNTANVVTGVHNFQLYGGTI+GILKGFLGLSGAVLIQFSNTFY+G+P++YLLM
Sbjct: 121 AVHAQVFFNTANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYNGDPINYLLM 180

Query: 181 LAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWA 240
           LAILPALTT+LLMRFVVI KTE GNESTHLNSLS IALIIS YLTILI+LDNVL LPTW 
Sbjct: 181 LAILPALTTVLLMRFVVIQKTEIGNESTHLNSLSKIALIISCYLTILIILDNVLILPTWV 240

Query: 241 RLFTFILLLALLACPLGIAVRAQTEDSVFKTKLQNTDDCVEYHRIPSEDQRNEQLTIVTD 300
           RLFTF++LL LLA PLGIA RAQTEDS  KTKLQ+TD+ VEYH+IP E+QRN+QL +VT+
Sbjct: 241 RLFTFVILLILLASPLGIANRAQTEDSASKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTN 300

Query: 301 GEMNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWN 360
           GEMN+IEA+GTVNFW+LF AMMCGMGSGLATINNMNQLGQSLGY TVEI+TFVSLWSIWN
Sbjct: 301 GEMNVIEAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWN 360

Query: 361 FLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYG 420
           FLGRLGSGYASDLLL +LGWARPLLMA+ALLTMSVGHIIIASG S NLYLGSVIVGICYG
Sbjct: 361 FLGRLGSGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGSSWNLYLGSVIVGICYG 420

Query: 421 AQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK 480
           +QWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK
Sbjct: 421 SQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGK 480

Query: 481 HCFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLKD 523
           HCFMTSFLIMAAVAFLGFVVA ALF RTRRFYQ+LVQR+LKD
Sbjct: 481 HCFMTSFLIMAAVAFLGFVVAFALFLRTRRFYQLLVQRQLKD 521

BLAST of Lag0034175 vs. NCBI nr
Match: KAG7021495.1 (Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 891.0 bits (2301), Expect = 5.1e-255
Identity = 457/515 (88.74%), Postives = 486/515 (94.37%), Query Frame = 0

Query: 7    SNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGLLAGL 66
            SNRWIAT++SIWIQCICGPSYTFGIYSSALKS+QNY QSTLDTVSVFKDIGATAG+LAGL
Sbjct: 546  SNRWIATVASIWIQCICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGL 605

Query: 67   LYSAVVSHDRPRRPWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHAQV 126
            LYSAVVS DRP RPWIVL VGAIQCFLGYIF+WAAVSGLIPRP V  MCLFMFLAVHAQV
Sbjct: 606  LYSAVVSTDRPHRPWIVLSVGAIQCFLGYIFIWAAVSGLIPRPSVSTMCLFMFLAVHAQV 665

Query: 127  FFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVDYLLMLAILPA 186
            FFNTANVVTGVHNFQLYGGTI+GILKGFLGLSGAVLIQFSNTFY G+P+ YLLMLAILPA
Sbjct: 666  FFNTANVVTGVHNFQLYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPA 725

Query: 187  LTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWARLFTFI 246
            LTT+LLMRFVVI+KTE GNESTHLNSLS IALIIS YLTILI+LDNV  L TW RLFTF+
Sbjct: 726  LTTVLLMRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFILTTWVRLFTFV 785

Query: 247  LLLALLACPLGIAVRAQTEDSVFKTKLQNTDDCVEYHRIPSEDQRNEQLTIVTDGEMNII 306
            LLL LLA PLGIA RAQTEDSV KTKLQ+TD+ VEYH+IP E+QRN+QL +VT+GEMN+I
Sbjct: 786  LLLILLASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVI 845

Query: 307  EAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWNFLGRLG 366
            EA+GTVNFW+LF AMMCGMGSGLATINNMNQLGQSLGY TVEI+TFVSLWSIWNFLGRLG
Sbjct: 846  EAIGTVNFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLG 905

Query: 367  SGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYGAQWSLM 426
            SGYASDLLL +LGWARPLLMA+ALLTMSVGHIIIASGFSGNLYLGSVIVGICYG+QWSLM
Sbjct: 906  SGYASDLLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLM 965

Query: 427  PAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTS 486
            PAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTS
Sbjct: 966  PAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTS 1025

Query: 487  FLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLK 522
            FLIMAAVAFLGFVVAVALF RTRRFYQ+L +R+ K
Sbjct: 1026 FLIMAAVAFLGFVVAVALFLRTRRFYQLLGKRKRK 1060

BLAST of Lag0034175 vs. NCBI nr
Match: XP_022933362.1 (uncharacterized protein LOC111440723 [Cucurbita moschata])

HSP 1 Score: 878.2 bits (2268), Expect = 3.4e-251
Identity = 451/501 (90.02%), Postives = 478/501 (95.41%), Query Frame = 0

Query: 22  ICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGLLAGLLYSAVVSHDRPRRPW 81
           ICGPSYTFGIYSSALKS+QNY QSTLDTVSVFKDIGATAG+LAGLLYSAVVS DRPRRPW
Sbjct: 13  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW 72

Query: 82  IVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 141
           IVL VGAIQCFLGYIF+WAAVSGLIPRPPV AMCLFMFLAVHAQVFFNTANVVTGVHNFQ
Sbjct: 73  IVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 132

Query: 142 LYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVDYLLMLAILPALTTLLLMRFVVIDKT 201
           LYGGTI+GILKGFLGLSGAVLIQFSNTFY G+P+ YLLMLAILPALTT+LLMRFVVI+KT
Sbjct: 133 LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT 192

Query: 202 ETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWARLFTFILLLALLACPLGIAVR 261
           E GNESTHLNSLS IALIIS YLTILI+LDNV  LPTW RLFTF+LLL LLA PLGIA R
Sbjct: 193 EIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLGIANR 252

Query: 262 AQTEDSVFKTKLQNTDDCVEYHRIPSEDQRNEQLTIVTDGEMNIIEAMGTVNFWILFFAM 321
           AQTEDSV KTKLQ+TD+ VEYH+IP E+QRN+QL +VT+GEMN+IEA+GTVNFW+LF AM
Sbjct: 253 AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 312

Query: 322 MCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWA 381
           MCGMGSGLATINNMNQLGQSLGY TVEI+TFVSLWSIWNFLGRLGSGYASDLLL +LGWA
Sbjct: 313 MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 372

Query: 382 RPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYGAQWSLMPAITAEIFGIRHMGT 441
           RPLLMA+ALLTMSVGHIIIASGFSGNLYLGSVIVGICYG+QWSLMPAITAEIFGIRHMGT
Sbjct: 373 RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 432

Query: 442 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFVVA 501
           IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGF+VA
Sbjct: 433 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFIVA 492

Query: 502 VALFFRTRRFYQVLVQRRLKD 523
           VALF RTRRFYQ+LVQR+LKD
Sbjct: 493 VALFLRTRRFYQLLVQRQLKD 513

BLAST of Lag0034175 vs. NCBI nr
Match: XP_022134701.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 [Momordica charantia])

HSP 1 Score: 840.5 bits (2170), Expect = 7.9e-240
Identity = 435/524 (83.02%), Postives = 473/524 (90.27%), Query Frame = 0

Query: 1   MEAPSLSNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATA 60
           ME  SLSNRWIAT++SIWIQCICGPSYTFGIYSSALKSTQ Y QSTLDTVSVF D+GATA
Sbjct: 1   MEVHSLSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQAYDQSTLDTVSVFXDVGATA 60

Query: 61  GLLAGLLYSAVVSHDRPRRPWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFL 120
           G+LA LLYSAV S++RPRR WI+  VGAIQCF GYIF+WAAVSGLIPRPPVPAMC FMFL
Sbjct: 61  GVLAVLLYSAVASNNRPRRSWIIHTVGAIQCFAGYIFIWAAVSGLIPRPPVPAMCFFMFL 120

Query: 121 AVHAQVFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVDYLLM 180
           AVHAQVFFNTANVVTGVHNFQLY  TIVGILKGF GL GAVLIQFSNTF + +P + L++
Sbjct: 121 AVHAQVFFNTANVVTGVHNFQLYSATIVGILKGFAGLXGAVLIQFSNTFCNRDPANNLVL 180

Query: 181 LAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWA 240
           L+ILPAL+TLLLM FVVIDKTETGNES HLNS S+IALIIS YLTILI+L+N LTLP WA
Sbjct: 181 LSILPALSTLLLMAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWA 240

Query: 241 RLFTFILLLALLACPLGIAVRAQTEDSVFKTKLQNTDDCVEYHRIPSEDQRNEQLTIVTD 300
           R+FTF+LLL L+A PLGIA+RA+TEDSVF   LQN DD VEY +IPSE Q ++QL IV+D
Sbjct: 241 RIFTFVLLLTLIASPLGIAIRARTEDSVF---LQNADDSVEYRQIPSEVQSDDQLAIVSD 300

Query: 301 GEMNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWN 360
           GEMNIIEA+GTVNFW+L FAMMCGMGSGLATINNMNQLGQSLGYGTVEIN FVSLWSIWN
Sbjct: 301 GEMNIIEAIGTVNFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWN 360

Query: 361 FLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYG 420
           FLGRLG GYASDLLL +LG ARP LMA ALLTMS+GHIIIASGF GNLY+GSVIVGICYG
Sbjct: 361 FLGRLGPGYASDLLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYG 420

Query: 421 AQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDRE--ASAGDNSCS 480
           +QWSLMPAITAEIFGIR+MGTI+NTITVASPI SY+LSVRVIGY YDRE  ASAGDNSCS
Sbjct: 421 SQWSLMPAITAEIFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCS 480

Query: 481 GKHCFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLKD 523
           GKHCFMTSFL+MAAVAFLGF+VAV LFFRTRRFYQ LVQRRLKD
Sbjct: 481 GKHCFMTSFLVMAAVAFLGFLVAVVLFFRTRRFYQSLVQRRLKD 521

BLAST of Lag0034175 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.6e-25
Identity = 134/544 (24.63%), Postives = 237/544 (43.57%), Query Frame = 0

Query: 9   RWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGLLAGLLY 68
           +W   +++IWIQ   G ++ F  YSS LKS     Q  L+ ++V  D+G   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 69  SAVVSHDRPRRPWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHAQVFF 128
                        +VL   A   F+GY   W  ++ +I   P   + L   LA  +  +F
Sbjct: 103 GYFPLS-------VVLFAAAAMGFVGYGVQWLVITNIITL-PYSLVFLCCLLAGLSICWF 162

Query: 129 NTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVDYLLMLAILPALT 188
           NTA  +  + +F       + +   F G+S A+     N     +   YLL+ +++P + 
Sbjct: 163 NTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVV 222

Query: 189 TLLLMRFVV----IDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWARLFT 248
           +   +  V+    +D T   +   H + + +I  +++   +  ++L +  T  + ARL  
Sbjct: 223 SFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSST--SSARL-N 282

Query: 249 FILLLALLACPLGIAVRAQTED---SVFKTKLQ---------NTDDCVEYHRIPSEDQRN 308
           FI  + LL  PL   +     D    V   +L          N D+        S     
Sbjct: 283 FIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGY 342

Query: 309 EQLTIVTDG-------EMNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYG 368
           E +    +G       E +    +  + FW+ + A  CG   GL   NN+ Q+ QSLG  
Sbjct: 343 EHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQN 402

Query: 369 TVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFS 428
           +    T V+++S ++F GRL S  A D +  +    R    AIALL   +   ++A   S
Sbjct: 403 S---TTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSS 462

Query: 429 GN--LYLGSVIVGICYGAQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIG 488
               L   + ++G+  G  ++   +IT+++FG   +G  +N +    PIGS +L   +  
Sbjct: 463 QQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIAA 522

Query: 489 YIYDREAS-------AGDNSCSGKHCFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLV 521
            IY+  AS       +    C G+ C+  +F+    ++ LG V +++L+ RT+  Y  L 
Sbjct: 523 SIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHRLE 570

BLAST of Lag0034175 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 7.2e-10
Identity = 54/188 (28.72%), Postives = 100/188 (53.19%), Query Frame = 0

Query: 336 NQLGQSLGYGTVE------INTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIA 395
           N +G  L   TVE       +T VSL++ ++ + RL  G++S+ +  +   +RP+L+++ 
Sbjct: 293 NNMGAILDTVTVENADSPSFSTHVSLFATFSTVSRLVVGFSSEAM--ESHVSRPVLLSVI 352

Query: 396 LLTMSVGHIIIASG----FSGNLYLG--SVIVGICYGAQWSLMPAITAEIFGIRHMGTIY 455
            L  +  H+++ SG    F    Y    +++ G  YG+ ++L+P I  +++GI ++GTI+
Sbjct: 353 ALVAACIHLMVPSGIFTVFDNAKYFSVVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIW 412

Query: 456 NTITVASPIGSYVLSVRVIGYIYDREASAGDNS----CSGKHCFMTSFLIM-AAVAFLGF 507
            +  +A  +GS    + +   +YD  +  G  S    CSG HC+  +F+I    +AF   
Sbjct: 413 GSFILALAVGSLGYGL-LFAKVYDAASEVGVGSMSQVCSGVHCYGLTFVITGTGLAF--- 472

BLAST of Lag0034175 vs. ExPASy Swiss-Prot
Match: Q6FWD4 (Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 3.7e-06
Identity = 61/249 (24.50%), Postives = 108/249 (43.37%), Query Frame = 0

Query: 276 TDDCVEYHRIPSEDQRNEQLTIVTDGEMNIIEAMGTVNFWILFFAMMCGMGSGLATINNM 335
           +D+ VE H   +E  R    T     +M +++       +I    + C +G     I NM
Sbjct: 244 SDNDVENHYDDNEQSRLLHATHAQ--QMTLMKVFRDPVLYIFGATIFCALGPLEMFIANM 303

Query: 336 NQLGQSL--GYGTVEINTFVSLWSIWNFLGRLGSGYASDLL---LWKLGWARPLLMAIAL 395
             L   L  G+     +  +S++++ + L RLG+G   D        + W   L + + L
Sbjct: 304 GSLTNVLAGGHEPAMSSALLSIYALTSTLTRLGTGLTVDYFNKRQLSVKWILLLFLVVGL 363

Query: 396 LTMSVGHIIIASG--------FSGNLYLGSVIVGICYGAQWSLMPAITAEIFGIRHMGTI 455
           +T    +++  S          +  L+   ++ GI YG  +++ P IT  ++G +  GT 
Sbjct: 364 VTQGKIYMLSMSSLDHSHMVTINRKLFYIGIMQGIAYGGLFTIYPTITLMVWGEKMFGTA 423

Query: 456 YNTITVASPIGSYVLSVRVIGYIYDRE-ASAGDNSCSGKHCFMTSFLIMAAVAFLGFVVA 511
           Y T+ +A  +GS  LS  +   +YD E A++   SC       T+    AA+      V 
Sbjct: 424 YGTLMIAPALGS-ALSCLIYADVYDSECANSTTRSCIAPVYETTALEFCAAIL---LTVV 483

BLAST of Lag0034175 vs. ExPASy Swiss-Prot
Match: A0LNN5 (L-lactate transporter OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=Sfum_3364 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 4.1e-05
Identity = 52/203 (25.62%), Postives = 85/203 (41.87%), Query Frame = 0

Query: 307 EAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWNFLGRLG 366
           EA G   FW+L+ A  CG  +GL  I ++   G+  G   +     VS  +  N   R+ 
Sbjct: 223 EAKGDTKFWLLYLAYFCGSFAGLMVIGHLAGFGRDAGLTAMAAAGAVSSLAFSNAATRIL 282

Query: 367 SGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYGAQWSLM 426
           SG+  D    K+G         AL T ++  I    G    L + ++++G  YGA ++L 
Sbjct: 283 SGWFVD----KIGIRVYFAALFALQTAAMIAIFQLGGSVVGLSIVAIVIGWNYGAMFTLF 342

Query: 427 PAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTS 486
           PA   + +G    G+ Y  +  A  +  +     V G++ D   +           +   
Sbjct: 343 PATCLQFYGPTAQGSNYGLLFTACGLAGFA-GPWVGGWLKDTTGT-----------YYLP 402

Query: 487 FLIMAAVAFLGFVVAVALFFRTR 510
           FL  AA+  LG     A+ F T+
Sbjct: 403 FLCAAALCALG----TAIVFMTK 405

BLAST of Lag0034175 vs. ExPASy TrEMBL
Match: A0A6J1EZJ6 (uncharacterized protein LOC111440723 OS=Cucurbita moschata OX=3662 GN=LOC111440723 PE=4 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.6e-251
Identity = 451/501 (90.02%), Postives = 478/501 (95.41%), Query Frame = 0

Query: 22  ICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGLLAGLLYSAVVSHDRPRRPW 81
           ICGPSYTFGIYSSALKS+QNY QSTLDTVSVFKDIGATAG+LAGLLYSAVVS DRPRRPW
Sbjct: 13  ICGPSYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPRRPW 72

Query: 82  IVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 141
           IVL VGAIQCFLGYIF+WAAVSGLIPRPPV AMCLFMFLAVHAQVFFNTANVVTGVHNFQ
Sbjct: 73  IVLSVGAIQCFLGYIFIWAAVSGLIPRPPVSAMCLFMFLAVHAQVFFNTANVVTGVHNFQ 132

Query: 142 LYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVDYLLMLAILPALTTLLLMRFVVIDKT 201
           LYGGTI+GILKGFLGLSGAVLIQFSNTFY G+P+ YLLMLAILPALTT+LLMRFVVI+KT
Sbjct: 133 LYGGTIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKT 192

Query: 202 ETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWARLFTFILLLALLACPLGIAVR 261
           E GNESTHLNSLS IALIIS YLTILI+LDNV  LPTW RLFTF+LLL LLA PLGIA R
Sbjct: 193 EIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILLASPLGIANR 252

Query: 262 AQTEDSVFKTKLQNTDDCVEYHRIPSEDQRNEQLTIVTDGEMNIIEAMGTVNFWILFFAM 321
           AQTEDSV KTKLQ+TD+ VEYH+IP E+QRN+QL +VT+GEMN+IEA+GTVNFW+LF AM
Sbjct: 253 AQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFWLLFSAM 312

Query: 322 MCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWA 381
           MCGMGSGLATINNMNQLGQSLGY TVEI+TFVSLWSIWNFLGRLGSGYASDLLL +LGWA
Sbjct: 313 MCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASDLLLRELGWA 372

Query: 382 RPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYGAQWSLMPAITAEIFGIRHMGT 441
           RPLLMA+ALLTMSVGHIIIASGFSGNLYLGSVIVGICYG+QWSLMPAITAEIFGIRHMGT
Sbjct: 373 RPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAEIFGIRHMGT 432

Query: 442 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFVVA 501
           IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGF+VA
Sbjct: 433 IYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFIVA 492

Query: 502 VALFFRTRRFYQVLVQRRLKD 523
           VALF RTRRFYQ+LVQR+LKD
Sbjct: 493 VALFLRTRRFYQLLVQRQLKD 513

BLAST of Lag0034175 vs. ExPASy TrEMBL
Match: A0A6J1BYJ5 (LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 OS=Momordica charantia OX=3673 GN=LOC111006908 PE=4 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.8e-240
Identity = 435/524 (83.02%), Postives = 473/524 (90.27%), Query Frame = 0

Query: 1   MEAPSLSNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATA 60
           ME  SLSNRWIAT++SIWIQCICGPSYTFGIYSSALKSTQ Y QSTLDTVSVF D+GATA
Sbjct: 1   MEVHSLSNRWIATVASIWIQCICGPSYTFGIYSSALKSTQAYDQSTLDTVSVFXDVGATA 60

Query: 61  GLLAGLLYSAVVSHDRPRRPWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFL 120
           G+LA LLYSAV S++RPRR WI+  VGAIQCF GYIF+WAAVSGLIPRPPVPAMC FMFL
Sbjct: 61  GVLAVLLYSAVASNNRPRRSWIIHTVGAIQCFAGYIFIWAAVSGLIPRPPVPAMCFFMFL 120

Query: 121 AVHAQVFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVDYLLM 180
           AVHAQVFFNTANVVTGVHNFQLY  TIVGILKGF GL GAVLIQFSNTF + +P + L++
Sbjct: 121 AVHAQVFFNTANVVTGVHNFQLYSATIVGILKGFAGLXGAVLIQFSNTFCNRDPANNLVL 180

Query: 181 LAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWA 240
           L+ILPAL+TLLLM FVVIDKTETGNES HLNS S+IALIIS YLTILI+L+N LTLP WA
Sbjct: 181 LSILPALSTLLLMAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWA 240

Query: 241 RLFTFILLLALLACPLGIAVRAQTEDSVFKTKLQNTDDCVEYHRIPSEDQRNEQLTIVTD 300
           R+FTF+LLL L+A PLGIA+RA+TEDSVF   LQN DD VEY +IPSE Q ++QL IV+D
Sbjct: 241 RIFTFVLLLTLIASPLGIAIRARTEDSVF---LQNADDSVEYRQIPSEVQSDDQLAIVSD 300

Query: 301 GEMNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWN 360
           GEMNIIEA+GTVNFW+L FAMMCGMGSGLATINNMNQLGQSLGYGTVEIN FVSLWSIWN
Sbjct: 301 GEMNIIEAIGTVNFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWN 360

Query: 361 FLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYG 420
           FLGRLG GYASDLLL +LG ARP LMA ALLTMS+GHIIIASGF GNLY+GSVIVGICYG
Sbjct: 361 FLGRLGPGYASDLLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYG 420

Query: 421 AQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDRE--ASAGDNSCS 480
           +QWSLMPAITAEIFGIR+MGTI+NTITVASPI SY+LSVRVIGY YDRE  ASAGDNSCS
Sbjct: 421 SQWSLMPAITAEIFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCS 480

Query: 481 GKHCFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLKD 523
           GKHCFMTSFL+MAAVAFLGF+VAV LFFRTRRFYQ LVQRRLKD
Sbjct: 481 GKHCFMTSFLVMAAVAFLGFLVAVVLFFRTRRFYQSLVQRRLKD 521

BLAST of Lag0034175 vs. ExPASy TrEMBL
Match: A0A5A7UT75 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002270 PE=4 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 8.1e-190
Identity = 348/544 (63.97%), Postives = 425/544 (78.12%), Query Frame = 0

Query: 1   MEAPS-LSNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGAT 60
           MEA + + N+W AT+  IWIQCICG SYTF IYSSALKSTQ+Y QSTLDTVSVFKDIGA 
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGLLAGLLYSAVVSHDRPRR----PWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMC 120
           AG+++G LYSAV   + PRR    PW+V   GAIQ FLGYIF+WAAVSG+I RPPVPAMC
Sbjct: 61  AGIISGFLYSAVTPLN-PRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMC 120

Query: 121 LFMFLAVHAQVFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPV 180
            FMFLA HAQ FFNTANVVTGVHNF  Y GTIVGI+KG+LGLSGA+LIQ  NT  + +P 
Sbjct: 121 FFMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPS 180

Query: 181 DYLLMLAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLT 240
           ++LLMLA+LP + +++ M FV IDKTE+ NE  HLNSLS++A+I++ YL ++I+L+N  +
Sbjct: 181 NFLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFS 240

Query: 241 LPTWARLFTFILLLALLACPLGIAVRAQTED--------------SVFKTKLQNTDDCVE 300
           L +W R FTF +LL LLA PLGIA+ AQ ED               V K +  + +D VE
Sbjct: 241 LSSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVE 300

Query: 301 YHRIPSEDQRNEQLTIVTDGE----MNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQ 360
           YH +P E+   +Q+ +V++      MN++EA+ T+NFW+LF AM+CGMGSGLATINNM+Q
Sbjct: 301 YHELPREE---DQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQ 360

Query: 361 LGQSLGYGTVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGH 420
           LGQSLGY   E  TFVSLWSIWNFLGR G+GY SD L    GWARPLLMAI LL MS GH
Sbjct: 361 LGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGH 420

Query: 421 IIIASGFSGNLYLGSVIVGICYGAQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVL 480
           I+IASGFSGNLY+GS++VGICYG+QWSLMP IT+EIFG+ HMGTI+NTI +ASP+GSY+ 
Sbjct: 421 IVIASGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIF 480

Query: 481 SVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQ 522
           SVRVIGYIYDREA+    +CSG HCF+ SFL+MA VAFLGF+VA ALFFRTRRFY++ +Q
Sbjct: 481 SVRVIGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQ 540

BLAST of Lag0034175 vs. ExPASy TrEMBL
Match: A0A1S3BWC2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 8.1e-190
Identity = 348/544 (63.97%), Postives = 425/544 (78.12%), Query Frame = 0

Query: 1   MEAPS-LSNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGAT 60
           MEA + + N+W AT+  IWIQCICG SYTF IYSSALKSTQ+Y QSTLDTVSVFKDIGA 
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGLLAGLLYSAVVSHDRPRR----PWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMC 120
           AG+++G LYSAV   + PRR    PW+V   GAIQ FLGYIF+WAAVSG+I RPPVPAMC
Sbjct: 61  AGIISGFLYSAVTPLN-PRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMC 120

Query: 121 LFMFLAVHAQVFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPV 180
            FMFLA HAQ FFNTANVVTGVHNF  Y GTIVGI+KG+LGLSGA+LIQ  NT  + +P 
Sbjct: 121 FFMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMKGYLGLSGALLIQVYNTTCNADPS 180

Query: 181 DYLLMLAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLT 240
           ++LLMLA+LP + +++ M FV IDKTE+ NE  HLNSLS++A+I++ YL ++I+L+N  +
Sbjct: 181 NFLLMLAVLPTVLSIMFMWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFS 240

Query: 241 LPTWARLFTFILLLALLACPLGIAVRAQTED--------------SVFKTKLQNTDDCVE 300
           L +W R FTF +LL LLA PLGIA+ AQ ED               V K +  + +D VE
Sbjct: 241 LSSWTRYFTFSILLILLAAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVE 300

Query: 301 YHRIPSEDQRNEQLTIVTDGE----MNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQ 360
           YH +P E+   +Q+ +V++      MN++EA+ T+NFW+LF AM+CGMGSGLATINNM+Q
Sbjct: 301 YHELPREE---DQIMVVSNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQ 360

Query: 361 LGQSLGYGTVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGH 420
           LGQSLGY   E  TFVSLWSIWNFLGR G+GY SD L    GWARPLLMAI LL MS GH
Sbjct: 361 LGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGH 420

Query: 421 IIIASGFSGNLYLGSVIVGICYGAQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVL 480
           I+IASGFSGNLY+GS++VGICYG+QWSLMP IT+EIFG+ HMGTI+NTI +ASP+GSY+ 
Sbjct: 421 IVIASGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIF 480

Query: 481 SVRVIGYIYDREASAGDNSCSGKHCFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQ 522
           SVRVIGYIYDREA+    +CSG HCF+ SFL+MA VAFLGF+VA ALFFRTRRFY++ +Q
Sbjct: 481 SVRVIGYIYDREAAGEHGACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQ 540

BLAST of Lag0034175 vs. ExPASy TrEMBL
Match: A0A6J1C134 (uncharacterized protein LOC111007401 OS=Momordica charantia OX=3673 GN=LOC111007401 PE=4 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 1.4e-189
Identity = 350/534 (65.54%), Postives = 419/534 (78.46%), Query Frame = 0

Query: 6   LSNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGLLAG 65
           +SN+WIAT  SIWIQC+ G SYTF IYS+ALKSTQ Y QSTLDTVSVFKDIGA AG+++G
Sbjct: 9   MSNKWIATALSIWIQCVSGASYTFSIYSAALKSTQGYDQSTLDTVSVFKDIGANAGVVSG 68

Query: 66  LLYSAVVSHDRPRR---------PWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCL 125
            LY+A V+   PRR         PWIV   GAIQ F+GYIF+WAAVSG+I RPPVPAMC 
Sbjct: 69  FLYTA-VTPATPRRSSPARFLSGPWIVHAGGAIQWFVGYIFIWAAVSGVIDRPPVPAMCF 128

Query: 126 FMFLAVHAQVFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVD 185
           FMFLA HAQ FFNTANVVTGVHNF  Y GTIVGI+KG+LGLSGA+LIQ  NTF +G P +
Sbjct: 129 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKGYLGLSGALLIQVYNTFCNGEPSN 188

Query: 186 YLLMLAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTL 245
            LLMLA+LP L +LL+M FV IDKTET NE  HLNSLS++A+I++ YL I+I+L+N   L
Sbjct: 189 LLLMLAVLPTLLSLLVMWFVRIDKTETSNEMKHLNSLSALAVILAFYLMIVIILENTFPL 248

Query: 246 PTWARLFTFILLLALLACPLGIAVRAQTED------SVFKTKLQNTDDCVEYHRIPSEDQ 305
            +W RL TF +LL LLA PLGI++ AQ ED      S +  K    D+ +EYH + S++ 
Sbjct: 249 SSWTRLLTFSILLILLAAPLGISINAQREDFGGSSPSPYTEKSPVLDEPLEYHELASDE- 308

Query: 306 RNEQLTIVTD---GEMNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTV 365
            ++Q+ +        MN++EA+ ++NFW+LFFAM+CGMGSGLATINNM+QLGQSLGY   
Sbjct: 309 -SQQIAVSNSRAPRAMNVLEAIRSINFWLLFFAMVCGMGSGLATINNMSQLGQSLGYTVT 368

Query: 366 EINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGN 425
           E  TFVSLWSIWNFLGR G+GY SD LL   GWARPLLMAI LL MS GHI+IASGFSGN
Sbjct: 369 ETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGN 428

Query: 426 LYLGSVIVGICYGAQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYD 485
           LY+GSV+VGICYG+QW LMP IT+EIFG+ HMGTI+NTI +ASP+GSY+ SVRVIGYIYD
Sbjct: 429 LYVGSVLVGICYGSQWGLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYD 488

Query: 486 REASAGDNSCSGKHCFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRLK 522
           REA+    SCSG HCF+ SFL+MAAVAFLGF+VA ALFFRTRRFY++  QRR++
Sbjct: 489 REAAGKHGSCSGTHCFVVSFLVMAAVAFLGFLVAAALFFRTRRFYRLAFQRRVE 539

BLAST of Lag0034175 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 590.9 bits (1522), Expect = 1.0e-168
Identity = 308/528 (58.33%), Postives = 391/528 (74.05%), Query Frame = 0

Query: 6   LSNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGLLAG 65
           L  +W+A  +SIWIQC  G SYTFGIYS+ LKSTQ+Y QSTLDTVSVFKDIGA AG+ +G
Sbjct: 4   LRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSG 63

Query: 66  LLYSAVVSHDRPRR---------PWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCL 125
           LLY+   S+    R         PW+VL VGAIQCF GY  +WA+V+GLI +PPVP MCL
Sbjct: 64  LLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCL 123

Query: 126 FMFLAVHAQVFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVD 185
           FMFLA  +Q FFNTANVV+ V NF  YGGT VGI+KGFLGLSGA+LIQ   T   G+P  
Sbjct: 124 FMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDPAS 183

Query: 186 YLLMLAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTL 245
           ++L+LA+ P + +LL+M  V I +T   ++  HLN LS+++LII+ YL I+I+L N   L
Sbjct: 184 FILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGL 243

Query: 246 PTWARLFTFILLLALLACPLGIAVRAQTEDSVFKTKLQNTDDCVEYHR-IPSEDQRNEQL 305
            +WA + T + LL +LA PL IA RAQ  D + KT   +    +   +   S +Q +E  
Sbjct: 244 SSWANIVTLVCLLVMLALPLLIARRAQ-RDGMEKTVPHDYSPLISSPKATTSGNQSSEGD 303

Query: 306 TIVTDG---EMNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTF 365
           + V  G    +N+++AM  ++FW+LF AM+CGMGSGL+TINN+ Q+G+SL Y +VEIN+ 
Sbjct: 304 SKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINSL 363

Query: 366 VSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGS 425
           VSLWSIWNFLGR G+GYASD LL K GW RPLLMA  L TMS+GH+IIASGF GNLY+GS
Sbjct: 364 VSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGS 423

Query: 426 VIVGICYGAQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASA 485
           VIVG+CYG+QWSLMP IT+E+FGIRHMGTI+NTI+VASPIGSY+ SVR+IGYIYD+ AS 
Sbjct: 424 VIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYDKTASG 483

Query: 486 GDNSCSGKHCFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRL 521
             N+C G HCF  SF+IMA+VAF GF+VA+ LFFRT+  Y+ ++ +RL
Sbjct: 484 EGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQILVKRL 530

BLAST of Lag0034175 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 554.3 bits (1427), Expect = 1.1e-157
Identity = 284/527 (53.89%), Postives = 381/527 (72.30%), Query Frame = 0

Query: 1   MEAPSLSNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATA 60
           M    L  +W+A  +SIWIQC  G SYTFGIYS+ LKSTQ+Y QSTLDTVSVFKDIG   
Sbjct: 1   MATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNV 60

Query: 61  GLLAGLLYSAVVSHDRPR-------RPWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPA 120
           G+L+GL+Y+A   + R R        PW+V+++GAI  F GY  MWA+V+GLI RPPVP 
Sbjct: 61  GVLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPV 120

Query: 121 MCLFMFLAVHAQVFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGN 180
           MCLFMF+A  +  F NTANVV+ + NF  YGGT VGI+KGF+GLSGA+LIQ       G+
Sbjct: 121 MCLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGD 180

Query: 181 PVDYLLMLAILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNV 240
           P  ++L+LAI+P+L ++L+M  V + KT T +E  HL+ LS+++LII+ YL I I+L + 
Sbjct: 181 PKTFILLLAIVPSLLSVLVMPLVRVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKST 240

Query: 241 LTLPTWARLFTFILLLALLACPLGIAVRAQTEDSVFKTKLQNTDDCVEYHRIPSEDQRNE 300
           L+LP+WA   T  +LL LL+ PL +AVRA   DS+ K         V+     +     E
Sbjct: 241 LSLPSWANAVTLAVLLVLLSSPLLVAVRAH-RDSIEKPLSSVYSPLVDNLEATTS---GE 300

Query: 301 QLTIVTDGEMNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFV 360
            L +  D  +N+++AM  V+FW+LF AM+CGMGSG++TINN+ Q+G+SL Y +VEIN+ +
Sbjct: 301 ILMLDEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLRYTSVEINSLL 360

Query: 361 SLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSV 420
           +LW+IWNF+GR G GY SD LL + GW RPLLMA  L TM++GH+IIASGF GNLY GS+
Sbjct: 361 ALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIASGFQGNLYPGSI 420

Query: 421 IVGICYGAQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAG 480
           IVGICYG+QWSLMP IT+E+FG++HMGTIYNTI++ASP+GSY+ SVR+IGYIYDR     
Sbjct: 421 IVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLIGYIYDRTIIGE 480

Query: 481 DNSCSGKHCFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRL 521
            N+C G HCF  +++++A+VAFLGF+V+  L FRT+  Y+ + ++ L
Sbjct: 481 GNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYRQIFEKIL 523

BLAST of Lag0034175 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 546.2 bits (1406), Expect = 2.9e-155
Identity = 285/522 (54.60%), Postives = 375/522 (71.84%), Query Frame = 0

Query: 7   SNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGLLAGL 66
           + +W+A  +SIWIQ   G SYTFGIYSS LKS+Q+Y QSTLDTVSV+KDIGA  G+L+GL
Sbjct: 5   NTKWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILSGL 64

Query: 67  LYSAVVSHDRPR-----RPWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLA 126
            Y+AV S           PW+V+ VG +Q F+GY F+W A SG+IPRPPV  MCLFMF A
Sbjct: 65  FYTAVASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMFFA 124

Query: 127 VHAQVFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVDYLLML 186
            H Q FFNTA VVT V NF  YGGT VGI+KG+LGLSGA+L+Q  + F  G+P +Y+L+L
Sbjct: 125 GHCQPFFNTAIVVTAVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYILLL 184

Query: 187 AILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWAR 246
           A++P+L  L LM FV    T    +  HLN LS+I+LII  YL ++I+++N++ +    +
Sbjct: 185 AVVPSLLILTLMPFVRTYDTVIAGDKKHLNGLSAISLIIVTYLMVVILVENIIGMSMPMK 244

Query: 247 LFTFILLLALLACPLGIAVRAQTEDSVFKTKLQNTDDCVEYHRIPSEDQRNEQLTIVTD- 306
           + +F  LL LLA PL +AVRAQ E+   + +  + D  V       +  +    + V D 
Sbjct: 245 ICSFTFLLLLLASPLLVAVRAQREE---EHRFLSLDFPVTERTTLLDSPKLNSSSDVKDV 304

Query: 307 --GEMNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSI 366
              +MN++EA+ T NFW+LF AM+CGMGSGLATINN+ Q+G+SL Y TV++N+ VSLWSI
Sbjct: 305 MTNDMNVLEAICTTNFWLLFVAMICGMGSGLATINNIRQMGESLRYSTVQLNSLVSLWSI 364

Query: 367 WNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGIC 426
           WNFLGR GSGY SD  L   GW RP+ MAI L  M++GHI++ASG  G+LY+GS++VG+ 
Sbjct: 365 WNFLGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVMASGLLGSLYIGSLLVGLA 424

Query: 427 YGAQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCS 486
           YG+QWSLMP IT+EIFG+ HMGTI+ TI++ASP+GSY  SV+VIGY+YD+ AS  D+SC 
Sbjct: 425 YGSQWSLMPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVKVIGYLYDKVASEDDHSCY 484

Query: 487 GKHCFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRRL 521
           G HCF TSFLIMAA+A LG +VA+ L  RT++FY  LV +R+
Sbjct: 485 GNHCFRTSFLIMAAMALLGSLVALVLLLRTKKFYATLVAKRI 523

BLAST of Lag0034175 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 545.8 bits (1405), Expect = 3.7e-155
Identity = 288/518 (55.60%), Postives = 375/518 (72.39%), Query Frame = 0

Query: 6   LSNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGLLAG 65
           ++ +W+A  +SIWIQ   G +YTF IYSS LKS+Q+Y QSTLD VSVFKDIG T G+++G
Sbjct: 4   INTKWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISG 63

Query: 66  LLYSAVVSHDRP-RRPWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHA 125
            LY+A+ S  R    PW+V+ VG +Q F+G+ F+WA+V GLI  PPVP MCLF+FLA H+
Sbjct: 64  FLYTAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHS 123

Query: 126 QVFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFY--DGNPVDYLLMLA 185
             FFNTANVVT   NF  YGGT VGI++GFLGLSGA+LIQ  +     +GNP  ++L+LA
Sbjct: 124 LPFFNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLA 183

Query: 186 ILPALTTLLLMRFVVIDKTETGNESTHLNSLSSIALIISGYLTILIVLDNVLTLPTWARL 245
           I+P L   L M FV + +T T ++  HL+ LS+I++II+ YL ++I ++NVL L    ++
Sbjct: 184 IVPTLVMFLAMPFVRVYETVTISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQI 243

Query: 246 FTFILLLALLACPLGIAVRAQTEDSVFKTKLQN-TDDCVEYHRIPSEDQRNEQLTIVTDG 305
           F+FIL+L LLA PL +AVRA  E     + L     D       PS +   +   +V + 
Sbjct: 244 FSFILVLLLLASPLLVAVRALREKRQTLSSLDGPVLDTSALLDPPSSNIFPDGDHLVAE- 303

Query: 306 EMNIIEAMGTVNFWILFFAMMCGMGSGLATINNMNQLGQSLGYGTVEINTFVSLWSIWNF 365
           + NI+EAM TVNFW+LF AM+CGMGSG AT+NNM Q+G+SL Y +V++N+ VSLWSIWNF
Sbjct: 304 DSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNSLVSLWSIWNF 363

Query: 366 LGRLGSGYASDLLLWKLGWARPLLMAIALLTMSVGHIIIASGFSGNLYLGSVIVGICYGA 425
           LGR G+GY SD  L K  W RP+ MAI L  M++GHII+ASG  G+LY GSV++G+ YG+
Sbjct: 364 LGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAGSVLIGMAYGS 423

Query: 426 QWSLMPAITAEIFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREASAGDNSCSGKH 485
           QWSLMP IT+EIFGIRHMGTIY TI++A PIGSY+LSV+VIGY YD+ AS  DNSC G  
Sbjct: 424 QWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKVASEDDNSCFGSQ 483

Query: 486 CFMTSFLIMAAVAFLGFVVAVALFFRTRRFYQVLVQRR 520
           CF TSF+IMA+VA  G +VA  LFFRT +FY+ LV +R
Sbjct: 484 CFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVAKR 520

BLAST of Lag0034175 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 1.5e-87
Identity = 200/559 (35.78%), Postives = 310/559 (55.46%), Query Frame = 0

Query: 6   LSNRWIATISSIWIQCICGPSYTFGIYSSALKSTQNYHQSTLDTVSVFKDIGATAGLLAG 65
           L+ RW     S+ I    G +Y FGIYS  +K T  Y Q+TL+ +S FKD+GA  G+LAG
Sbjct: 18  LTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAG 77

Query: 66  LLYSAVVSHDRPRRPWIVLIVGAIQCFLGYIFMWAAVSGLIPRPPVPAMCLFMFLAVHAQ 125
           LL       +    PW +L++GAI  F GY  +W AV+  I +P V  MCL++ +  ++Q
Sbjct: 78  LL-------NEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQ 137

Query: 126 VFFNTANVVTGVHNFQLYGGTIVGILKGFLGLSGAVLIQFSNTFYDGNPVDYLLMLAILP 185
            F NT ++VT V NF    G ++GILKG++GLSGA++ Q    FY  +  + +LM+  LP
Sbjct: 138 SFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLP 197

Query: 186 ALTTLLLMRFVVIDKTE-TGNESTHLNSLSSIALIISGYLTILIVLDNV--LTLPTWARL 245
           A+ +   +R + I K +   NE     +   I+L ++ +L ++I+++ +   T   +   
Sbjct: 198 AIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGS 257

Query: 246 FTFILLLALLACPLGIAVRAQTEDSVFKTKLQNTDDCVEYHRIPSEDQRNEQLTIVTDGE 305
              +++L LL     I V    E  ++K K    +D    + +  + + +       DGE
Sbjct: 258 AAVVIVLLLLP----IIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGE 317

Query: 306 ---------------------------MNIIEAMGTVNFWILFFAMMCGMGSGLATINNM 365
                                        I++A+ +V+  ILF A +CG+G  L  I+N+
Sbjct: 318 ESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNL 377

Query: 366 NQLGQSLGYGTVEINTFVSLWSIWNFLGRLGSGYASDLLLWKLGWARPLLMAIALLTMSV 425
            Q+G SLGY    ++TFVSL SIWN+ GR+ SG  S++ L K  + RPL++ + LL    
Sbjct: 378 GQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCA 437

Query: 426 GHIIIASGFSGNLYLGSVIVGICYGAQWSLMPAITAEIFGIRHMGTIYNTITVASPIGSY 485
           GH++IA    G LY+ SVI+G C+GAQW L+ AI +EIFG+++  T+YN  +VASPIGSY
Sbjct: 438 GHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSY 497

Query: 486 VLSVRVIGYIYDREA------------SAGDNSCSGKHCFMTSFLIMAAVAFLGFVVAVA 523
           +L+VRV GY+YD EA               D +C G  CF  SF+I+AAV   G +V++ 
Sbjct: 498 LLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMV 557