Lag0034174 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034174
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionMajor facilitator superfamily
Locationchr3: 5028583 .. 5034645 (+)
RNA-Seq ExpressionLag0034174
SyntenyLag0034174
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGTTTTAGGGTTTATCACCAACAAATGGATTGCAACGGCCCTCGGGATTTGGATCCAATGCATCTGCGGTGCCTCCTACACCTTCAGCATCTATTCTTCGGCTCTCAAATCCACTCAGGGCTACGATCAATCCACCCTCGACACCGTTTCCGTTTTCAAGGACATCGGCGCCAACGCCGGCGTCATTTCTGGCTTTCTCTACTCCGCCGTCACTCCCGCCTCCCGCCGATCGAGCTCCGCTGGCCCATGGGTGGTCCACGCGGCGGGAGCGGTTCAGTGCTTCCTCGGATATATTTTCATCTGGGCGGCCGTCTCCGGTGTGATCGATCGGCCGCCGGTGCCGGCGATGTGCTTCTTCATGTTTCTGGCTGCACATGCGCAAACGTTTTTCAATACCGCGAATGTGGTGACCGGCGTTCACAATTTTGCGCACTATAGCGGGACGATTGTCGGCATCATGAAGGTACCAACACAAACTCTTAGTTTTCTTGATTATACAGATTGTTGTGTTGGGACTCTGGATTTTGGCTTCGATTTTAGCTTAGATTGTTGGGGAAATTTGATTCTGGTGAGTATTATTGATTCAGATACTGTAGAGAGATCATGTAGGCTACAACTAAATCCATGTCCTTGAGTGATTAAGTTATGGAGATGAACTTTGTCTTTCCTTCCTCTGTTCTTCTCTGCCATTATCCATTTTTTTTTTATCAAGGATGGTGGGTCTTGGAAATAATCCAGTTTTGATTAAAAACTGTGGCCAGATCTAATCCCCAAGTTGTTGGAAATAAGAGGTTAAACATGTTCTGGTCTATTAAACTTGAACCTAACCTAAAAAAAAATATAGCACCAGGTTCAATAGCCTTCCAGTTTTTGAAAGCTAAGCCTATAAACACTACTTCTTCCTATAGTTTTCTTGTTTTATTATCTACCGGTTCTGGAATATGCTAGTTATGAATTGAACATGTTACATCCTGTGTTTCCCTTTTGTTGGATAGGGATATCTTGGTCTTAGTGGAGCACTGCTAATCCAAGTTTATAACACGGCGTGCAATGGAGAACCAAGCAATTTTCTCCTGATGCTGGCTGTATTGCCCACACTTCTTTCCGTCATGTTCATGTGGTTTGTTAGAATTGACAAAACAGAGTCCAGAAATGAGATGAAGCATTTGAACTCATTGTCTGCGCTTGCGGTGATTGTTGCTTTTTACTTGATGGTTGTCATCATTTTGAACAACACTTTCACCCTTTCATCATGGACTCGTTTCATTACATTCTCCATACTCTTGATTCTCCTTGCTGCTCCTCTTGGAATCGCCATTAATGCTCAGAGGGAGGAGAACTTTAGAGGGTCATCTCCATCACTCTTCACTGGAGAAAGTCCTGTTCTTAACAAACCCAAGTCAGTGAATGCAGAGGATTCTGTGGAATATCACGAGTTGCCGAGTGATGAGAGCCAGAGAATGGCTGCTGCAAACTCCAAACCTCCTCAAGCCATGAATGTCTTGGAAGCTATACGCACTGTAAACTTCTGGCTTTTGTTTCTTGCCATGGTGTGTGGAATGGGTTCTGGATTGGCCACAATAAACAACATGAGCCAGCTTGGCCAATCCCTTGGCTACACAGCAACAGAGACAAAGACATTCGTTTCCTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGCTACACCTCTGATTTTCTTCTCCACTCATATGGCTGGGCAAGGCCATTGTTAATGGCAATTACTCTTCTGATAATGAGTGGTGGCCACATCGTGATCGCCTCTGGTTTCTCTGGAAATCTCTATGTGGGTTCAATTCTTGTTGGCATATGCTACGGCTCACAGTGGTCACTGATGCCCACAATCACTTCAGAGATATTTGGCTTAGAGCATATGGGAACTATTTTCAACACCATAGCCATAGCCAGTCCTCTGGGGTCTTACATCTTTTCGGTTCGAGTCATCGGCTACATTTACGACCGAGAAGCCGCCGGTAAACATGGCTCCTGCTCCGGCACACACTGTTTTGTAGTATCTTTTCTGGTCATGGCCGCCGTGGCTTTCTTGGGTTTTCTTGTTGCTGCTGCTCTCTTCTTTCGAACCAGGAGATTTTACCGACTGGCTTTGCAAAGAAGAGTTGAACGTCTCAATTGAAGGAGCCAGAGAGAAATAGGATCAGATAATTGCTTATTGATTTAATAGAATTCTGGAAGGATTTGGTTTTGAGTGTAAAGAAATTCATTGAAGTTTCATAATTCGTGGTTCTTTACTTATAAACAGAATATGAGAGTGAGATCAAATGTATTTTTAAACGTTGTATTGTATCATAGTTTGATCAAGAAACTGCTTGGATCAAACTGTCAACACATTTATTTCCTTTTGATTTTTGTTCCTTTCTCTTGCACTAGAAGGAAGGAAGGAAAAAAGAAAAAAACTTAATATTTGTAAATATTATATGGCGTGATTGTGAACCTTGCCGTGTATATTTTATTTGAAGCAATATGTTTTTATATGTAGTGTTTTTGACAACGTGAAAAGATTCCCAATTATTATAATATATTTCGATTTCCATGCCTACTTATATTTAGATTTTAGGAAATATTTTATGTTTGAATCTTGGTTAATTATAAAAAATGCCCCTCAATTATGGGGTTTGTTTCAAAAATGCCTCTGAACTTTAAAAAATTACAAAAATACCCTTGAACTTTAAAAAATGTTTAAAAAATACGTTAATTTTTTTATGGAAACTATTAAAAATTTCAGTTTCATTAATACTAACTTTCAAAATATTTTAAAAAATAACCTTATTCGAAAATGCTAATTTTTTTTCTTCTCTCACCGTTAATTCTTCTTCCATGAAAATTAATGAGAAACTTTACCTATTTTTCATGTGTTCATAACAATGGTATTTTCGAAACATTTTTAACGGTTTTAACGGTTCCCATCCAAAAATTAATTGCAAGGATATTTTTGAAACGTTTTGTAAAGTTCAAGGGTATTTTTGAAATTTTTTAAAGTTCAGGGGCATTTTTTAAACAAACCCCATAGTTTAGGAGCATTTTTTTATAATTTAGCCTTGAATCTTATAACGCCATAGTGGCTGCCACCCTGTCCACATGACACTTGCCTGTATGATTTATTATTATTTTTTTTTTTTTTTTGAAAAGGCCTGTATGATTTATTTGCTCTCTGAAAAGACTGTAATTTTGTTTTTTTTTTTTTTTTTTGGAAAAGACTGTAATTTTGTTTTTTTACAGTCTAAAATTACGTAACAACTACATAATACATATATCTCAGATTTATTTACAATCTACTATTTGTGCCACATGTATATTTACTATATTTAAAAAAAAAAAAAAAAAACATGTCTATTTACTACTGTGACTCTGACCCAAAATGTTTGAATCTCCCGTTCAATAAATAAGGGTTTGTTTGATAGGCCATCCGAATTCTGTTTTATGTTTTCAGAATATACGTTTGGCAATTGTGTTTTTTAAAACGGGTATTTCGGATTATGTGATCCAAATAGCGAAAATTCTGAAACAACTTTTTTTATGTTCAGTTTATCCAAATTTTGAATTTGAAAATTTTAAATGCAGAATTAAATTAAAAAAAAAACAAACAATTAATAAATATAGTATTTCATGGTATAAATTCAATTCATTTTTATGAAATTAAAATTTGTATAATATAATGTATTATAAATTATAGGATATTATAAAATAATAATTATACAAAATTTATGACATATAAAATGTTTATAAATTAATTAATAATAATTTATATTCAACTTAATATTTAGTTGATGACTAAAAATAGTTTATAATTTATAAATATATTATGAAACACATCTCGAACCATTTTTGAAATTAGAATCCAATTTCTGAATATGCTACCAAATACATAATCCAATTTCAAATTTTTGTTTATCACCTACCAAAACGCACCTAAGTTTTCAAACAAGTGTTTGGGTTGGTTCTGCAATATTGAGTCTGAAATTAATGGAGGAATAGTCTTGAGTTTAAAAAAAATAAAAATAAAAATTTTTAGAATGGAGATTTAACGAAAATATATAGGCAACTAAAAAATTACGATGCGAAAAGAAGAAGGAAAAAGGAGATCGCAGTACCTGAATTCATCTTGAGATGGAAGCTCCTTCTCTGAGCAACAGATAGATCGCTACAGTTGCAAGCATTTGGATCCAATGCATCTGCGGCCCATCCTACACCTTCGGCCGGAGTTCTCGCCGGCCTCCTCTACTCCGCCGTTGTCTCCACCAATCACCCGCGTCGCCCATGGATCGTCCTCATCGTCGGAGCAATCCAGTGCTTCCTCGGTTACATTTTCATATGGCCCGCCGTCTCCGGCCTAATCCCTCGGCTGCCGGTGCCGGCGATGTGCCTGTTCATGTTTCTAGCCGTCCATGCCCAAGTTTTCTTCAACACGGCCAACGTCGTCACTGGTGTCAATAACTTCCAGCTCTACGGCGGCACCATTCTCGGCATTCAAAAGGTCCTTATCTCTCTCCCTCTCTCTAAAAGATTTTCAATTTCAATATCTCATAATCGATGATTCAACAGGGATTTCTAGGTCTCAGTGGAGCAGTTCTGATTCAATTCTCGAACACACTCTGCAATCGAGACCCTGTAGCATTTCTTTTAATGCTTGCGATCTTGCCGGCGCTCACGACGCTTCTGCTCATGAGATTCGTCGTCATCGACAAAACAGAGACCGGAAACGAATCCAAACATTTGAACTCCTTCTCGTCAATCGCTCTGATAATCTCCGGTTATCTCACGATTCTCATCATTTTAGACAATTCCCTCACCCTTCCAACATGGGCTCGCATCTTCACCTTCGTTCTACTCCTAACCCTCATCGCTTCCCCTCTCGGAATCGCAATCAGAGCAAAAACAGAGGACTCTGTTTTCAGAACCAAGCTTGACGAATCCCTTGAATACCGTCAAATTCCCAGTGAAGAACAGACCCACGATCAGCAAGTGCTCGATTCCGATAGAGACATTGATCTAATCGAGGCCACTGGAACTTTAGACTTCTGGGTATTGTTTCTTTCAATAATGTGCTCGATGGGTTCTGGATTGGCCACCATAAACAACATGAATCAACTCGGCCAATGCGCAGTCTAGATCAACACCTTGGTTTCCTTGTGGAGCATTTGGAGTTTCCTTGGCCGCCTTCAAATTTTCGAACCCAAAATTTAAGGTCCATTTGATAATCCTTTTTTTTATTTTTGAAAATTTAAGGTACTAGTTTTCAAAACTTGTTTTTAGAATTTGGTTAGGAATTCAAATGGATCATTAACAAAGATGAAAGCATGGTAGGAGAAAAGAAACATAATTTTCAAAAACCAAAAACAAAAAACGAAGCCCCGTTCGATAACCGATTTTTGGTTTTTGAAAATTAAGCTTATACACACATACTTCTACCTATAAGTATTTTGGTTTTAGTATCAATCTTCTATCTTATTTCCAAAAACCAAGTCCAATTTTGGAAACAAAAAAAATAGTTTTCAAAAACTTGTTTTTGTTTTTAAATTTTGGCTAGTAATTTAAGTGCAACCTTAACTATGATGAAAGTCATGGTAGGGAATTTAGGAAAAAACAAGTTTAATTTTAAAAAACCAAATGGTTATCCAACGGGCCTAAGTGGTTAGATTACTGTAAACTTAATCATTCATTCATATGCTTGACAGGTTACGTTTCTGATATTCTCCTACGCAAATTAGGCTGGGGAAGGCCATTGTTGATGGCCATAACTCTGCTAGCAATGAGCGTCGGCCACATCATAATTGCTTTGGGCTTCACTGGGAATTTATACGTTGGATCAGTGATGGTGGGGATTTGTTATGGAGCTCAAATCTCTCTGATGCCTGCAATTACGGCGGAGATATTTGGAATAAAGCATATGGGCACCATTTACAACGCCATCAATTGGATCTTATATTTTATCTGTTCGAATCGTTGGGTTTATATATGACAGAGAAGCCTCTTCTGAAGACAACTCCTGCTCCGGCAAACACTGTTTTATGA

mRNA sequence

ATGGAAGTTTTAGGGTTTATCACCAACAAATGGATTGCAACGGCCCTCGGGATTTGGATCCAATGCATCTGCGGTGCCTCCTACACCTTCAGCATCTATTCTTCGGCTCTCAAATCCACTCAGGGCTACGATCAATCCACCCTCGACACCGTTTCCGTTTTCAAGGACATCGGCGCCAACGCCGGCGTCATTTCTGGCTTTCTCTACTCCGCCGTCACTCCCGCCTCCCGCCGATCGAGCTCCGCTGGCCCATGGGTGGTCCACGCGGCGGGAGCGGTTCAGTGCTTCCTCGGATATATTTTCATCTGGGCGGCCGTCTCCGGTGTGATCGATCGGCCGCCGGTGCCGGCGATGTGCTTCTTCATGTTTCTGGCTGCACATGCGCAAACGTTTTTCAATACCGCGAATGTGGTGACCGGCGTTCACAATTTTGCGCACTATAGCGGGACGATTGTCGGCATCATGAAGGTACCAACACAAACTCTTAGTTTTCTTGATTATACAGATTGTTGTGTTGGGACTCTGGATTTTGGCTTCGATTTTAGCTTAGATTGTTGGGGAAATTTGATTCTGGGATATCTTGGTCTTAGTGGAGCACTGCTAATCCAAGTTTATAACACGGCGTGCAATGGAGAACCAAGCAATTTTCTCCTGATGCTGGCTGTATTGCCCACACTTCTTTCCGTCATGTTCATGTGGTTTGTTAGAATTGACAAAACAGAGTCCAGAAATGAGATGAAGCATTTGAACTCATTGTCTGCGCTTGCGGTGATTGTTGCTTTTTACTTGATGGTTGTCATCATTTTGAACAACACTTTCACCCTTTCATCATGGACTCGTTTCATTACATTCTCCATACTCTTGATTCTCCTTGCTGCTCCTCTTGGAATCGCCATTAATGCTCAGAGGGAGGAGAACTTTAGAGGGTCATCTCCATCACTCTTCACTGGAGAAAGTCCTGTTCTTAACAAACCCAAGTCAGTGAATGCAGAGGATTCTGTGGAATATCACGAGTTGCCGAGTGATGAGAGCCAGAGAATGGCTGCTGCAAACTCCAAACCTCCTCAAGCCATGAATGTCTTGGAAGCTATACGCACTGTAAACTTCTGGCTTTTGTTTCTTGCCATGGTGTGTGGAATGGGTTCTGGATTGGCCACAATAAACAACATGAGCCAGCTTGGCCAATCCCTTGGCTACACAGCAACAGAGACAAAGACATTCGTTTCCTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGCTACACCTCTGATTTTCTTCTCCACTCATATGGCTGGGCAAGGCCATTGTTAATGGCAATTACTCTTCTGATAATGAGTGGTGGCCACATCGTGATCGCCTCTGGTTTCTCTGGAAATCTCTATGTGGGTTCAATTCTTGTTGGCATATGCTACGGCTCACAGTGGTCACTGATGCCCACAATCACTTCAGAGATATTTGGCTTAGAGCATATGGGAACTATTTTCAACACCATAGCCATAGCCAGTCCTCTGGGGTCTTACATCTTTTCGGTTCGAGTCATCGGCTACATTTACGACCGAGAAGCCGCCGGTAAACATGGCTCCTGCTCCGGCACACACTGTTTTGTAGTATCTTTTCTGGTCATGGCCGCCGTGGCTTTCTTGGGTTTTCTTGTTGCTGCTGCTCTCTTCTTTCGAACCAGGAGATTTTACCGACTGGCTTTGCAAAGAAGAGTTGAACATAGATCGCTACAGTTGCAAGCATTTGGATCCAATGCATCTGCGGCCCATCCTACACCTTCGGCCGGAGTTCTCGCCGGCCTCCTCTACTCCGCCGTTGTCTCCACCAATCACCCGCGTCGCCCATGGATCGTCCTCATCGTCGGAGCAATCCAGTGCTTCCTCGGTTACATTTTCATATGGCCCGCCGTCTCCGGCCTAATCCCTCGGCTGCCGGTGCCGGCGATGTGCCTGTTCATGTTTCTAGCCGTCCATGCCCAAGTTTTCTTCAACACGGCCAACGTCGTCACTGGTGTCAATAACTTCCAGCTCTACGGCGGCACCATTCTCGGCATTCAAAAGGGATTTCTAGGTCTCAGTGGAGCAGTTCTGATTCAATTCTCGAACACACTCTGCAATCGAGACCCTGTAGCATTTCTTTTAATGCTTGCGATCTTGCCGGCGCTCACGACGCTTCTGCTCATGAGATTCGTCGTCATCGACAAAACAGAGACCGGAAACGAATCCAAACATTTGAACTCCTTCTCGTCAATCGCTCTGATAATCTCCGGTTATCTCACGATTCTCATCATTTTAGACAATTCCCTCACCCTTCCAACATGGGCTCGCATCTTCACCTTCGTTCTACTCCTAACCCTCATCGCTTCCCCTCTCGGAATCGCAATCAGAGCAAAAACAGAGGACTCTGTTTTCAGAACCAAGCTTGACGAATCCCTTGAATACCGTCAAATTCCCAGTGAAGAACAGACCCACGATCAGCAAGTGCTCGATTCCGATAGAGACATTGATCTAATCGAGGCCACTGGAACTTTAGACTTCTGGTCTAGATCAACACCTTGGTTTCCTTGTGGAGCATTTGGAGTTTCCTTGGCCGCCTTCAAATTTTCGAACCCAAAATTTAAGGTCCATTTGATAATCCTTTTTTTTATTTTTGAAAATTTAAGGCTGGGGAAGGCCATTGTTGATGGCCATAACTCTGCTAGCAATGAGCGTCGGCCACATCATAATTGCTTTGGGCTTCACTGGGAATTTATACGTTGGATCAGTGATGGTGGGGATTTGTTATGGAGCTCAAATCTCTCTGATGCCTGCAATTACGGCGGAGATATTTGGAATAAAGCATATGGGCACCATTTACAACGCCATCAATTGGATCTTATATTTTATCTGTTCGAATCGTTGGGTTTATATATGACAGAGAAGCCTCTTCTGAAGACAACTCCTGCTCCGGCAAACACTGTTTTATGA

Coding sequence (CDS)

ATGGAAGTTTTAGGGTTTATCACCAACAAATGGATTGCAACGGCCCTCGGGATTTGGATCCAATGCATCTGCGGTGCCTCCTACACCTTCAGCATCTATTCTTCGGCTCTCAAATCCACTCAGGGCTACGATCAATCCACCCTCGACACCGTTTCCGTTTTCAAGGACATCGGCGCCAACGCCGGCGTCATTTCTGGCTTTCTCTACTCCGCCGTCACTCCCGCCTCCCGCCGATCGAGCTCCGCTGGCCCATGGGTGGTCCACGCGGCGGGAGCGGTTCAGTGCTTCCTCGGATATATTTTCATCTGGGCGGCCGTCTCCGGTGTGATCGATCGGCCGCCGGTGCCGGCGATGTGCTTCTTCATGTTTCTGGCTGCACATGCGCAAACGTTTTTCAATACCGCGAATGTGGTGACCGGCGTTCACAATTTTGCGCACTATAGCGGGACGATTGTCGGCATCATGAAGGTACCAACACAAACTCTTAGTTTTCTTGATTATACAGATTGTTGTGTTGGGACTCTGGATTTTGGCTTCGATTTTAGCTTAGATTGTTGGGGAAATTTGATTCTGGGATATCTTGGTCTTAGTGGAGCACTGCTAATCCAAGTTTATAACACGGCGTGCAATGGAGAACCAAGCAATTTTCTCCTGATGCTGGCTGTATTGCCCACACTTCTTTCCGTCATGTTCATGTGGTTTGTTAGAATTGACAAAACAGAGTCCAGAAATGAGATGAAGCATTTGAACTCATTGTCTGCGCTTGCGGTGATTGTTGCTTTTTACTTGATGGTTGTCATCATTTTGAACAACACTTTCACCCTTTCATCATGGACTCGTTTCATTACATTCTCCATACTCTTGATTCTCCTTGCTGCTCCTCTTGGAATCGCCATTAATGCTCAGAGGGAGGAGAACTTTAGAGGGTCATCTCCATCACTCTTCACTGGAGAAAGTCCTGTTCTTAACAAACCCAAGTCAGTGAATGCAGAGGATTCTGTGGAATATCACGAGTTGCCGAGTGATGAGAGCCAGAGAATGGCTGCTGCAAACTCCAAACCTCCTCAAGCCATGAATGTCTTGGAAGCTATACGCACTGTAAACTTCTGGCTTTTGTTTCTTGCCATGGTGTGTGGAATGGGTTCTGGATTGGCCACAATAAACAACATGAGCCAGCTTGGCCAATCCCTTGGCTACACAGCAACAGAGACAAAGACATTCGTTTCCTTATGGAGTATATGGAACTTTCTTGGACGTTTTGGAGCTGGCTACACCTCTGATTTTCTTCTCCACTCATATGGCTGGGCAAGGCCATTGTTAATGGCAATTACTCTTCTGATAATGAGTGGTGGCCACATCGTGATCGCCTCTGGTTTCTCTGGAAATCTCTATGTGGGTTCAATTCTTGTTGGCATATGCTACGGCTCACAGTGGTCACTGATGCCCACAATCACTTCAGAGATATTTGGCTTAGAGCATATGGGAACTATTTTCAACACCATAGCCATAGCCAGTCCTCTGGGGTCTTACATCTTTTCGGTTCGAGTCATCGGCTACATTTACGACCGAGAAGCCGCCGGTAAACATGGCTCCTGCTCCGGCACACACTGTTTTGTAGTATCTTTTCTGGTCATGGCCGCCGTGGCTTTCTTGGGTTTTCTTGTTGCTGCTGCTCTCTTCTTTCGAACCAGGAGATTTTACCGACTGGCTTTGCAAAGAAGAGTTGAACATAGATCGCTACAGTTGCAAGCATTTGGATCCAATGCATCTGCGGCCCATCCTACACCTTCGGCCGGAGTTCTCGCCGGCCTCCTCTACTCCGCCGTTGTCTCCACCAATCACCCGCGTCGCCCATGGATCGTCCTCATCGTCGGAGCAATCCAGTGCTTCCTCGGTTACATTTTCATATGGCCCGCCGTCTCCGGCCTAATCCCTCGGCTGCCGGTGCCGGCGATGTGCCTGTTCATGTTTCTAGCCGTCCATGCCCAAGTTTTCTTCAACACGGCCAACGTCGTCACTGGTGTCAATAACTTCCAGCTCTACGGCGGCACCATTCTCGGCATTCAAAAGGGATTTCTAGGTCTCAGTGGAGCAGTTCTGATTCAATTCTCGAACACACTCTGCAATCGAGACCCTGTAGCATTTCTTTTAATGCTTGCGATCTTGCCGGCGCTCACGACGCTTCTGCTCATGAGATTCGTCGTCATCGACAAAACAGAGACCGGAAACGAATCCAAACATTTGAACTCCTTCTCGTCAATCGCTCTGATAATCTCCGGTTATCTCACGATTCTCATCATTTTAGACAATTCCCTCACCCTTCCAACATGGGCTCGCATCTTCACCTTCGTTCTACTCCTAACCCTCATCGCTTCCCCTCTCGGAATCGCAATCAGAGCAAAAACAGAGGACTCTGTTTTCAGAACCAAGCTTGACGAATCCCTTGAATACCGTCAAATTCCCAGTGAAGAACAGACCCACGATCAGCAAGTGCTCGATTCCGATAGAGACATTGATCTAATCGAGGCCACTGGAACTTTAGACTTCTGGTCTAGATCAACACCTTGGTTTCCTTGTGGAGCATTTGGAGTTTCCTTGGCCGCCTTCAAATTTTCGAACCCAAAATTTAAGGTCCATTTGATAATCCTTTTTTTTATTTTTGAAAATTTAAGGCTGGGGAAGGCCATTGTTGATGGCCATAACTCTGCTAGCAATGAGCGTCGGCCACATCATAATTGCTTTGGGCTTCACTGGGAATTTATACGTTGGATCAGTGATGGTGGGGATTTGTTATGGAGCTCAAATCTCTCTGATGCCTGCAATTACGGCGGAGATATTTGGAATAAAGCATATGGGCACCATTTACAACGCCATCAATTGGATCTTATATTTTATCTGTTCGAATCGTTGGGTTTATATATGACAGAGAAGCCTCTTCTGAAGACAACTCCTGCTCCGGCAAACACTGTTTTATGA

Protein sequence

MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGANAGVISGFLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFDFSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKTESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAINAQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNVLEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVVSFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVEHRSLQLQAFGSNASAAHPTPSAGVLAGLLYSAVVSTNHPRRPWIVLIVGAIQCFLGYIFIWPAVSGLIPRLPVPAMCLFMFLAVHAQVFFNTANVVTGVNNFQLYGGTILGIQKGFLGLSGAVLIQFSNTLCNRDPVAFLLMLAILPALTTLLLMRFVVIDKTETGNESKHLNSFSSIALIISGYLTILIILDNSLTLPTWARIFTFVLLLTLIASPLGIAIRAKTEDSVFRTKLDESLEYRQIPSEEQTHDQQVLDSDRDIDLIEATGTLDFWSRSTPWFPCGAFGVSLAAFKFSNPKFKVHLIILFFIFENLRLGKAIVDGHNSASNERRPHHNCFGLHWEFIRWISDGGDLLWSSNLSDACNYGGDIWNKAYGHHLQRHQLDLIFYLFESLGLYMTEKPLLKTTPAPANTVL
Homology
BLAST of Lag0034174 vs. NCBI nr
Match: KAG7021495.1 (Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1305.4 bits (3377), Expect = 0.0e+00
Identity = 703/890 (78.99%), Postives = 752/890 (84.49%), Query Frame = 0

Query: 1   MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60
           MEV+GF+TNKW+ATA+GIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVISGFLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGVISGFLYSAVTP +RR SSAGPWVVHAAGA+QCFLGYIFIWAAVSG IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPTTRRRSSAGPWVVHAAGAIQCFLGYIFIWAAVSGFIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFD 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMK                        
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMK------------------------ 180

Query: 181 FSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKT 240
                      GYLGLSGALLIQVYNTACNGEPSNFLLMLAV PTLLS+ FMWFVRID+T
Sbjct: 181 -----------GYLGLSGALLIQVYNTACNGEPSNFLLMLAVFPTLLSITFMWFVRIDRT 240

Query: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAIN 300
           ESRNEMKHLNSLSALAVIVAFYLMVVII NNTF+LSSWTRF+TFSILL+LL APLGIAIN
Sbjct: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSLSSWTRFVTFSILLVLLVAPLGIAIN 300

Query: 301 AQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNV 360
           AQRE+  R SSPS FT E+PVLNKPKS NAEDSVEYHELPSDE Q MAAANS+ P+AMNV
Sbjct: 301 AQRED-LRMSSPSFFTEENPVLNKPKSANAEDSVEYHELPSDELQTMAAANSRAPRAMNV 360

Query: 361 LEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420
           LEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF
Sbjct: 361 LEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420

Query: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480
           GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASG SGNLYVGSILVGICYGSQWSL
Sbjct: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGLSGNLYVGSILVGICYGSQWSL 480

Query: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVV 540
           MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAA KHGSCSGTHCFVV
Sbjct: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAEKHGSCSGTHCFVV 540

Query: 541 SFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVEH------------RSLQLQ----- 600
           SFLVMA VAFLG LVAAALFFRTRRFYRLALQRRVE              S+ +Q     
Sbjct: 541 SFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVERLTPPSNRWIATVASIWIQCICGP 600

Query: 601 --AFGSNASAAHPT------------------PSAGVLAGLLYSAVVSTNHPRRPWIVLI 660
              FG  +SA   +                   +AGVLAGLLYSAVVST+ P RPWIVL 
Sbjct: 601 SYTFGIYSSALKSSQNYDQSTLDTVSVFKDIGATAGVLAGLLYSAVVSTDRPHRPWIVLS 660

Query: 661 VGAIQCFLGYIFIWPAVSGLIPRLPVPAMCLFMFLAVHAQVFFNTANVVTGVNNFQLYGG 720
           VGAIQCFLGYIFIW AVSGLIPR  V  MCLFMFLAVHAQVFFNTANVVTGV+NFQLYGG
Sbjct: 661 VGAIQCFLGYIFIWAAVSGLIPRPSVSTMCLFMFLAVHAQVFFNTANVVTGVHNFQLYGG 720

Query: 721 TILGIQKGFLGLSGAVLIQFSNTLCNRDPVAFLLMLAILPALTTLLLMRFVVIDKTETGN 780
           TI+GI KGFLGLSGAVLIQFSNT  + DP+++LLMLAILPALTT+LLMRFVVI+KTE GN
Sbjct: 721 TIIGILKGFLGLSGAVLIQFSNTFYSGDPISYLLMLAILPALTTVLLMRFVVIEKTEIGN 780

Query: 781 ESKHLNSFSSIALIISGYLTILIILDNSLTLPTWARIFTFVLLLTLIASPLGIAIRAKTE 840
           ES HLNS S IALIIS YLTILIILDN   L TW R+FTFVLLL L+ASPLGIA RA+TE
Sbjct: 781 ESTHLNSLSKIALIISCYLTILIILDNVFILTTWVRLFTFVLLLILLASPLGIANRAQTE 840

Query: 841 DSVFRTKL---DESLEYRQIPSEEQTHDQQVLDSDRDIDLIEATGTLDFW 851
           DSV +TKL   D S+EY QIP EEQ +DQ ++ ++ ++++IEA GT++FW
Sbjct: 841 DSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTVNFW 854

BLAST of Lag0034174 vs. NCBI nr
Match: XP_022972528.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 973.4 bits (2515), Expect = 1.5e-279
Identity = 505/579 (87.22%), Postives = 526/579 (90.85%), Query Frame = 0

Query: 1   MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60
           MEV+GF+TNKW+ATA+GIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVISGFLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGVISGFLYSAVTPASRR SS GPWVVHAAGA+QCFLGYIFIWAAVSG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPASRRRSSVGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFD 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMK                        
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMK------------------------ 180

Query: 181 FSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKT 240
                      GYLGLSGALLIQVYNTACNG+PSNF+LMLAV PTLLS+MFMWFVRID+T
Sbjct: 181 -----------GYLGLSGALLIQVYNTACNGDPSNFVLMLAVFPTLLSIMFMWFVRIDRT 240

Query: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAIN 300
           ESRNEMKHLNSLSALAVIVAFYLMVVII NNTF+LSSWTRF+TFSILL+LL APLGIAIN
Sbjct: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSLSSWTRFVTFSILLVLLVAPLGIAIN 300

Query: 301 AQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNV 360
           AQRE+ FR SSPS FT E+PVLNKPKS NAEDSVEYHELPSDE Q MAAANS+ P+AMNV
Sbjct: 301 AQRED-FRMSSPSFFTEENPVLNKPKSANAEDSVEYHELPSDELQTMAAANSRAPRAMNV 360

Query: 361 LEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420
           LEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF
Sbjct: 361 LEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420

Query: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480
           GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL
Sbjct: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480

Query: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVV 540
           MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAA KHGSCSGTHCFVV
Sbjct: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAEKHGSCSGTHCFVV 540

Query: 541 SFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVEHRS 580
           SFLVMA VAFLG LVAAALFFRTRRFYRLALQRRVE  S
Sbjct: 541 SFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVERLS 543

BLAST of Lag0034174 vs. NCBI nr
Match: XP_023531919.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 970.7 bits (2508), Expect = 9.6e-279
Identity = 504/579 (87.05%), Postives = 525/579 (90.67%), Query Frame = 0

Query: 1   MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60
           MEV+GF+TNKW+ATA+GIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVISGFLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGVISGFLYSAVTPA+RR SS GPWVVHAAGA+QCFLGYIFIWAAVSG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPAARRRSSLGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFD 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMK                        
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMK------------------------ 180

Query: 181 FSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKT 240
                      GYLGLSGALLIQVYNTACNGEPSNFLLMLAV PTLLS+MFMWFVRID+T
Sbjct: 181 -----------GYLGLSGALLIQVYNTACNGEPSNFLLMLAVFPTLLSIMFMWFVRIDRT 240

Query: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAIN 300
           ESRNEMKHLNSLSALAVIVAFY+MVVII NNTF+LSSWTRF+TFSILL+LL APLGIAIN
Sbjct: 241 ESRNEMKHLNSLSALAVIVAFYVMVVIIFNNTFSLSSWTRFVTFSILLVLLVAPLGIAIN 300

Query: 301 AQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNV 360
           AQRE+  R SSPS FT E+PVLNKPKS NAEDSVEYHELPSDE Q MAAANS+ P+AMNV
Sbjct: 301 AQRED-LRMSSPSFFTEENPVLNKPKSANAEDSVEYHELPSDELQTMAAANSRAPRAMNV 360

Query: 361 LEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420
           LEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF
Sbjct: 361 LEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420

Query: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480
           GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL
Sbjct: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480

Query: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVV 540
           MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAA KHGSCSGTHCFVV
Sbjct: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAEKHGSCSGTHCFVV 540

Query: 541 SFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVEHRS 580
           SFLVMA VAFLG LVAAALFFRTRRFYRLALQRRVE  S
Sbjct: 541 SFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVERLS 543

BLAST of Lag0034174 vs. NCBI nr
Match: XP_022933853.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 965.3 bits (2494), Expect = 4.0e-277
Identity = 502/579 (86.70%), Postives = 521/579 (89.98%), Query Frame = 0

Query: 1   MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60
           MEV+GF+TNKW+ATA+GIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVISGFLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGVISGFLYSAVTP +RR SSAGPWVVHAAGA+QCFLGYIFIWAAVSG IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPTTRRRSSAGPWVVHAAGAIQCFLGYIFIWAAVSGFIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFD 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMK                        
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMK------------------------ 180

Query: 181 FSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKT 240
                      GYLGLSGALLIQVYNTACNGEPSNFLLMLAV PTLLS+ FMWFVRID+T
Sbjct: 181 -----------GYLGLSGALLIQVYNTACNGEPSNFLLMLAVFPTLLSITFMWFVRIDRT 240

Query: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAIN 300
           ESRNEMKHLNSLSALAVIVAFYLMV II NNTF+LSSWTRF+TFSILL+LL APLGIAIN
Sbjct: 241 ESRNEMKHLNSLSALAVIVAFYLMVAIIFNNTFSLSSWTRFVTFSILLVLLVAPLGIAIN 300

Query: 301 AQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNV 360
           AQRE+  R SSPS FT E+PVLNKPKS NAEDSVEYHELPSDE Q MAAANS+ P+AMNV
Sbjct: 301 AQRED-LRMSSPSFFTEENPVLNKPKSANAEDSVEYHELPSDELQTMAAANSRAPRAMNV 360

Query: 361 LEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420
           LEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF
Sbjct: 361 LEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420

Query: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480
           GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASG SGNLYVGSILVGICYGSQWSL
Sbjct: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGLSGNLYVGSILVGICYGSQWSL 480

Query: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVV 540
           MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAA KHGSCSGTHCFVV
Sbjct: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAEKHGSCSGTHCFVV 540

Query: 541 SFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVEHRS 580
           SFLVMA VAFLG LVAAALFFRTRRFYRLALQRRVE  S
Sbjct: 541 SFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVERLS 543

BLAST of Lag0034174 vs. NCBI nr
Match: KAG6587516.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 964.1 bits (2491), Expect = 9.0e-277
Identity = 502/579 (86.70%), Postives = 520/579 (89.81%), Query Frame = 0

Query: 1   MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60
           MEV GF+TNKW+ATA+GIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVTGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVISGFLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGVISGFLYSAVTP +RR SSAGPWVVHAAGA+QCF GYIFIWAAVSG IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPTTRRRSSAGPWVVHAAGAIQCFFGYIFIWAAVSGFIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFD 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMK                        
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMK------------------------ 180

Query: 181 FSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKT 240
                      GYLGLSGALLIQVYNTACNGEPSNFLLMLAV PTLLS+ FMWFVRID+T
Sbjct: 181 -----------GYLGLSGALLIQVYNTACNGEPSNFLLMLAVFPTLLSITFMWFVRIDRT 240

Query: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAIN 300
           ESRNEMKHLNSLSALAVIVAFYLMVVII NNTF+LSSWTRF+TFSILL+LL APLGIAIN
Sbjct: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSLSSWTRFVTFSILLVLLVAPLGIAIN 300

Query: 301 AQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNV 360
           AQRE+  R SSPS FT E+PVLNKPKS NAEDSVEYHELPSDE Q MAAANS+ P+AMNV
Sbjct: 301 AQRED-LRMSSPSFFTEENPVLNKPKSANAEDSVEYHELPSDELQTMAAANSRAPRAMNV 360

Query: 361 LEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420
           LEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF
Sbjct: 361 LEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420

Query: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480
           GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASG SGNLYVGSILVGICYGSQWSL
Sbjct: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGLSGNLYVGSILVGICYGSQWSL 480

Query: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVV 540
           MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAA KHGSCSGTHCFVV
Sbjct: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAEKHGSCSGTHCFVV 540

Query: 541 SFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVEHRS 580
           SFLVMA VAFLG LVAAALFFRTRRFYRLALQRRVE  S
Sbjct: 541 SFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVERLS 543

BLAST of Lag0034174 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 7.4e-24
Identity = 151/593 (25.46%), Postives = 252/593 (42.50%), Query Frame = 0

Query: 10  KWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGANAGVISGFLY 69
           KW      IWIQ   G ++ FS YSS LKS  G  Q  L+ ++V  D+G   G  SG + 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSG-IA 102

Query: 70  SAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAAHAQ 129
               P S         VV  A A   F+GY   W  ++ +I   P   +     LA  + 
Sbjct: 103 LGYFPLS---------VVLFAAAAMGFVGYGVQWLVITNIITL-PYSLVFLCCLLAGLSI 162

Query: 130 TFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFDFSLDCWGNL 189
            +FNTA  +  + +F +     +                                   +L
Sbjct: 163 CWFNTACFILCIRHFPNNRALAL-----------------------------------SL 222

Query: 190 ILGYLGLSGALLIQVYNTACNGEPSN-FLLMLAVLPTLLSVMFMWFV----RIDKTESRN 249
            + + G+S AL    +N A N   SN +LL+ +++P ++S   ++ V     +D T   +
Sbjct: 223 TVSFNGISAALYSLAFN-AINPSSSNLYLLLNSLVPLVVSFAALYPVLTKPSLDTTPDYD 282

Query: 250 EMKH----LNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLIL-LAAPLGI-- 309
             +H       L+ LAVI +F+L    +L+++ T S+   FI   +LL+  L APL +  
Sbjct: 283 SRRHDSHVFTILNVLAVITSFHL----LLSSSSTSSARLNFIGAVVLLVFPLCAPLLVYA 342

Query: 310 ------AINAQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAAN 369
                  INA+       SS  +      + N+  SV+++   E H   + E   +   +
Sbjct: 343 RDYFLPVINARLNHE---SSGYVMLNIDELKNQKTSVSSKTGYE-HMGTAKEGNTVRLGD 402

Query: 370 SKPPQAMNVLEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLW 429
               + +     I  + FWL ++A  CG   GL   NN+ Q+ QSLG  +T   T V+++
Sbjct: 403 EHSFRLL-----ISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNST---TLVTIY 462

Query: 430 SIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGN--LYVGSIL 489
           S ++F GR       DF+   +   R    AI LL       ++A   S    L   + L
Sbjct: 463 SSFSFFGRL-LSAAPDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATAL 522

Query: 490 VGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAA--- 549
           +G+  G  ++   +ITS++FG   +G   N +    P+GS ++   +   IY+  A+   
Sbjct: 523 IGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-IAASIYEANASPDI 570

Query: 550 ----GKHGSCSGTHCFVVSFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRV 576
                    C G  C+  +F+    ++ LG + + +L+ RT+  Y    Q +V
Sbjct: 583 TPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHRLEQDKV 570

BLAST of Lag0034174 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 2.1e-10
Identity = 58/206 (28.16%), Postives = 111/206 (53.88%), Query Frame = 0

Query: 370 WLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTF---VSLWSIWNFLGRFGAGYTS 429
           WL  L  V   G      NNM  +  ++     ++ +F   VSL++ ++ + R   G++S
Sbjct: 275 WLFLLCFVFIGGPFEMFQNNMGAILDTVTVENADSPSFSTHVSLFATFSTVSRLVVGFSS 334

Query: 430 DFLLHSYGWARPLLMAITLLIMSGGHIVIASG----FSGNLY--VGSILVGICYGSQWSL 489
           + +      +RP+L+++  L+ +  H+++ SG    F    Y  V +I+ G  YGS ++L
Sbjct: 335 EAMESHV--SRPVLLSVIALVAACIHLMVPSGIFTVFDNAKYFSVVTIVNGFSYGSSFTL 394

Query: 490 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGS----CSGTH 549
           +PTI ++++G+ ++GTI+ +  +A  +GS  + + +   +YD  +    GS    CSG H
Sbjct: 395 VPTIVTKVWGIANLGTIWGSFILALAVGSLGYGL-LFAKVYDAASEVGVGSMSQVCSGVH 454

Query: 550 CFVVSFLVM-AAVAFLGFLVAAALFF 562
           C+ ++F++    +AF     AAA+FF
Sbjct: 455 CYGLTFVITGTGLAF----AAAAVFF 473

BLAST of Lag0034174 vs. ExPASy Swiss-Prot
Match: Q96TW9 (Probable transporter MCH1 OS=Wickerhamomyces anomalus OX=4927 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 7.5e-08
Identity = 50/192 (26.04%), Postives = 93/192 (48.44%), Query Frame = 0

Query: 354 PPQAMNVLEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSI 413
           P      L+ I+ ++ ++L  +++  +G     I NM  L +++   +  +   V++ ++
Sbjct: 249 PNHKSKFLKFIKDISTYVLLFSLLLSIGPSEMYITNMGSLVKAITPNSLISDQ-VAIHAV 308

Query: 414 WNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASG--FSGNLYVGSILVG 473
           ++ L R   G  SDFL+ +Y  +R  L+   +++     I IA+        Y+ S L G
Sbjct: 309 FSTLSRLSLGALSDFLVTNYQISRSWLLLSIIVLGFFTQIFIATSTFVKDQYYIISALSG 368

Query: 474 ICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGS 533
             YG  ++L PT+   I+G E  G+ + +  IA  +GS  F + V G +YD  A G    
Sbjct: 369 FSYGGLFTLYPTVIFSIWGPEIFGSAWGSFMIAPAIGSTTFGM-VFGLVYD-SACGVFAE 428

Query: 534 CSGTHCFVVSFL 544
            +  +C  + FL
Sbjct: 429 STTGNCVSLVFL 437

BLAST of Lag0034174 vs. ExPASy Swiss-Prot
Match: Q6FWD4 (Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.4e-06
Identity = 60/250 (24.00%), Postives = 110/250 (44.00%), Query Frame = 0

Query: 330 AEDSVEYHELPSDESQRMAAANSKPPQAMNVLEAIRTVNFWLLFLAMVCGMGSGLATINN 389
           +++ VE H   +++S+ + A ++   Q M +++  R    ++    + C +G     I N
Sbjct: 244 SDNDVENHYDDNEQSRLLHATHA---QQMTLMKVFRDPVLYIFGATIFCALGPLEMFIAN 303

Query: 390 MSQLGQSL--GYTATETKTFVSLWSIWNFLGRFGAGYTSDFLLH---SYGWARPLLMAIT 449
           M  L   L  G+    +   +S++++ + L R G G T D+      S  W   L + + 
Sbjct: 304 MGSLTNVLAGGHEPAMSSALLSIYALTSTLTRLGTGLTVDYFNKRQLSVKWILLLFLVVG 363

Query: 450 LLIMSGGHIVIASGFSGN---------LYVGSILVGICYGSQWSLMPTITSEIFGLEHMG 509
           L+     +++  S    +          Y+G I+ GI YG  +++ PTIT  ++G +  G
Sbjct: 364 LVTQGKIYMLSMSSLDHSHMVTINRKLFYIG-IMQGIAYGGLFTIYPTITLMVWGEKMFG 423

Query: 510 TIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVVSFLVMAAVAFLGFLV 566
           T + T+ IA  LGS + S  +   +YD E A           +  + L   A   L   V
Sbjct: 424 TAYGTLMIAPALGSAL-SCLIYADVYDSECANSTTRSCIAPVYETTALEFCAAILL--TV 483

BLAST of Lag0034174 vs. ExPASy TrEMBL
Match: A0A6J1IA87 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471070 PE=4 SV=1)

HSP 1 Score: 973.4 bits (2515), Expect = 7.2e-280
Identity = 505/579 (87.22%), Postives = 526/579 (90.85%), Query Frame = 0

Query: 1   MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60
           MEV+GF+TNKW+ATA+GIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVISGFLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGVISGFLYSAVTPASRR SS GPWVVHAAGA+QCFLGYIFIWAAVSG+IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPASRRRSSVGPWVVHAAGAIQCFLGYIFIWAAVSGLIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFD 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMK                        
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMK------------------------ 180

Query: 181 FSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKT 240
                      GYLGLSGALLIQVYNTACNG+PSNF+LMLAV PTLLS+MFMWFVRID+T
Sbjct: 181 -----------GYLGLSGALLIQVYNTACNGDPSNFVLMLAVFPTLLSIMFMWFVRIDRT 240

Query: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAIN 300
           ESRNEMKHLNSLSALAVIVAFYLMVVII NNTF+LSSWTRF+TFSILL+LL APLGIAIN
Sbjct: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIIFNNTFSLSSWTRFVTFSILLVLLVAPLGIAIN 300

Query: 301 AQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNV 360
           AQRE+ FR SSPS FT E+PVLNKPKS NAEDSVEYHELPSDE Q MAAANS+ P+AMNV
Sbjct: 301 AQRED-FRMSSPSFFTEENPVLNKPKSANAEDSVEYHELPSDELQTMAAANSRAPRAMNV 360

Query: 361 LEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420
           LEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF
Sbjct: 361 LEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420

Query: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480
           GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL
Sbjct: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480

Query: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVV 540
           MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAA KHGSCSGTHCFVV
Sbjct: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAEKHGSCSGTHCFVV 540

Query: 541 SFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVEHRS 580
           SFLVMA VAFLG LVAAALFFRTRRFYRLALQRRVE  S
Sbjct: 541 SFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVERLS 543

BLAST of Lag0034174 vs. ExPASy TrEMBL
Match: A0A6J1F604 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111441144 PE=4 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.9e-277
Identity = 502/579 (86.70%), Postives = 521/579 (89.98%), Query Frame = 0

Query: 1   MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60
           MEV+GF+TNKW+ATA+GIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN
Sbjct: 1   MEVIGFMTNKWVATAIGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVISGFLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AGVISGFLYSAVTP +RR SSAGPWVVHAAGA+QCFLGYIFIWAAVSG IDRPPVPAMCF
Sbjct: 61  AGVISGFLYSAVTPTTRRRSSAGPWVVHAAGAIQCFLGYIFIWAAVSGFIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFD 180
           FMFLAAHAQTFFNTANVVTGVHNFAH+SGTIVGIMK                        
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFAHHSGTIVGIMK------------------------ 180

Query: 181 FSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKT 240
                      GYLGLSGALLIQVYNTACNGEPSNFLLMLAV PTLLS+ FMWFVRID+T
Sbjct: 181 -----------GYLGLSGALLIQVYNTACNGEPSNFLLMLAVFPTLLSITFMWFVRIDRT 240

Query: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAIN 300
           ESRNEMKHLNSLSALAVIVAFYLMV II NNTF+LSSWTRF+TFSILL+LL APLGIAIN
Sbjct: 241 ESRNEMKHLNSLSALAVIVAFYLMVAIIFNNTFSLSSWTRFVTFSILLVLLVAPLGIAIN 300

Query: 301 AQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNV 360
           AQRE+  R SSPS FT E+PVLNKPKS NAEDSVEYHELPSDE Q MAAANS+ P+AMNV
Sbjct: 301 AQRED-LRMSSPSFFTEENPVLNKPKSANAEDSVEYHELPSDELQTMAAANSRAPRAMNV 360

Query: 361 LEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420
           LEA+RT+NFWLLF+AMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF
Sbjct: 361 LEALRTLNFWLLFVAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420

Query: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480
           GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASG SGNLYVGSILVGICYGSQWSL
Sbjct: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGLSGNLYVGSILVGICYGSQWSL 480

Query: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVV 540
           MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAA KHGSCSGTHCFVV
Sbjct: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAEKHGSCSGTHCFVV 540

Query: 541 SFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVEHRS 580
           SFLVMA VAFLG LVAAALFFRTRRFYRLALQRRVE  S
Sbjct: 541 SFLVMAIVAFLGSLVAAALFFRTRRFYRLALQRRVERLS 543

BLAST of Lag0034174 vs. ExPASy TrEMBL
Match: A0A5A7UT75 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002270 PE=4 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 3.1e-267
Identity = 484/576 (84.03%), Postives = 510/576 (88.54%), Query Frame = 0

Query: 1   MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60
           ME   F+ NKW AT LGIWIQCICGASYTFSIYSSALKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVISGFLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP + R + AGPW+VHAAGA+Q FLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFD 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMK                        
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMK------------------------ 180

Query: 181 FSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKT 240
                      GYLGLSGALLIQVYNT CN +PSNFLLMLAVLPT+LS+MFMWFVRIDKT
Sbjct: 181 -----------GYLGLSGALLIQVYNTTCNADPSNFLLMLAVLPTVLSIMFMWFVRIDKT 240

Query: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAIN 300
           ES+NEMKHLNSLSALAVIVAFYLMVVIILNN F+LSSWTR+ TFSILLILLAAPLGIAIN
Sbjct: 241 ESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSLSSWTRYFTFSILLILLAAPLGIAIN 300

Query: 301 AQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNV 360
           AQ+E+ FRGS PSL T ES V+NKP+ ++AEDSVEYHELP +E Q M  +NS+ PQ MNV
Sbjct: 301 AQKED-FRGSPPSLITEESHVVNKPEPIDAEDSVEYHELPREEDQIMVVSNSRAPQPMNV 360

Query: 361 LEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420
           LEAIRT+NFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF
Sbjct: 361 LEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420

Query: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480
           GAGYTSDFL H+YGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL
Sbjct: 421 GAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480

Query: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVV 540
           MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAG+HG+CSGTHCFVV
Sbjct: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGACSGTHCFVV 540

Query: 541 SFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVE 577
           SFLVMA VAFLGFLVAAALFFRTRRFYRLALQRRVE
Sbjct: 541 SFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

BLAST of Lag0034174 vs. ExPASy TrEMBL
Match: A0A1S3BWC2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 3.1e-267
Identity = 484/576 (84.03%), Postives = 510/576 (88.54%), Query Frame = 0

Query: 1   MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60
           ME   F+ NKW AT LGIWIQCICGASYTFSIYSSALKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEASNFMHNKWTATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVISGFLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP + R + AGPW+VHAAGA+Q FLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPLNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFD 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMK                        
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMK------------------------ 180

Query: 181 FSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKT 240
                      GYLGLSGALLIQVYNT CN +PSNFLLMLAVLPT+LS+MFMWFVRIDKT
Sbjct: 181 -----------GYLGLSGALLIQVYNTTCNADPSNFLLMLAVLPTVLSIMFMWFVRIDKT 240

Query: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAIN 300
           ES+NEMKHLNSLSALAVIVAFYLMVVIILNN F+LSSWTR+ TFSILLILLAAPLGIAIN
Sbjct: 241 ESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSLSSWTRYFTFSILLILLAAPLGIAIN 300

Query: 301 AQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNV 360
           AQ+E+ FRGS PSL T ES V+NKP+ ++AEDSVEYHELP +E Q M  +NS+ PQ MNV
Sbjct: 301 AQKED-FRGSPPSLITEESHVVNKPEPIDAEDSVEYHELPREEDQIMVVSNSRAPQPMNV 360

Query: 361 LEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420
           LEAIRT+NFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF
Sbjct: 361 LEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420

Query: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480
           GAGYTSDFL H+YGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL
Sbjct: 421 GAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480

Query: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVV 540
           MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAG+HG+CSGTHCFVV
Sbjct: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEHGACSGTHCFVV 540

Query: 541 SFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVE 577
           SFLVMA VAFLGFLVAAALFFRTRRFYRLALQRRVE
Sbjct: 541 SFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

BLAST of Lag0034174 vs. ExPASy TrEMBL
Match: A0A0A0LUE8 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G008470 PE=4 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 1.1e-261
Identity = 477/579 (82.38%), Postives = 507/579 (87.56%), Query Frame = 0

Query: 1   MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60
           M+   F++NKWIAT LGIWIQCICGASYTFSIYSSALKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MDPSNFMSNKWIATVLGIWIQCICGASYTFSIYSSALKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVISGFLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCF 120
           AG+ISGFLYSAVTP + R + AGPW+VHAAGA+Q FLGYIFIWAAVSGVIDRPPVPAMCF
Sbjct: 61  AGIISGFLYSAVTPFNPRRAFAGPWMVHAAGAIQWFLGYIFIWAAVSGVIDRPPVPAMCF 120

Query: 121 FMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFD 180
           FMFLAAHAQTFFNTANVVTGVHNFA+YSGTIVGIMK                        
Sbjct: 121 FMFLAAHAQTFFNTANVVTGVHNFANYSGTIVGIMK------------------------ 180

Query: 181 FSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKT 240
                      GYLGLSGALLIQVYNT CN +PSNFLLMLAVLPT+LSVMFMWFVRIDKT
Sbjct: 181 -----------GYLGLSGALLIQVYNTTCNEDPSNFLLMLAVLPTVLSVMFMWFVRIDKT 240

Query: 241 ESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAIN 300
           ES NEMKHLNSLSALAVIVAFYLMVVIILNN F+LSSWTR+ TFSILLILLAAPLGIAIN
Sbjct: 241 ESSNEMKHLNSLSALAVIVAFYLMVVIILNNAFSLSSWTRYFTFSILLILLAAPLGIAIN 300

Query: 301 AQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNV 360
           AQ+E+ FRGSS SL   +S V+NKP+S++AEDSVEYHELP +E+Q M  +N++ PQ MNV
Sbjct: 301 AQKED-FRGSSSSLIAEKSHVVNKPESIDAEDSVEYHELPREENQIMVVSNTRAPQTMNV 360

Query: 361 LEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 420
           LEAIRT+NFWLLFLAMVCGMGSGLATINNMSQLGQSLGYT TETKTFVSLWSIWNFLGRF
Sbjct: 361 LEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTETETKTFVSLWSIWNFLGRF 420

Query: 421 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480
           GAGYTSDFL H+YGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL
Sbjct: 421 GAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 480

Query: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVV 540
           MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAA +HG+CSG HCFVV
Sbjct: 481 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAREHGACSGIHCFVV 540

Query: 541 SFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVEHRS 580
           SF VMA VAFLGFLVAAALFFRTRRFY+LA QRRV   S
Sbjct: 541 SFFVMAIVAFLGFLVAAALFFRTRRFYQLASQRRVNRVS 543

BLAST of Lag0034174 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 615.1 bits (1585), Expect = 9.5e-176
Identity = 327/584 (55.99%), Postives = 413/584 (70.72%), Query Frame = 0

Query: 1   MEVLGFITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGAN 60
           ME+L     KW+A    IWIQC  GASYTF IYS+ LKSTQ YDQSTLDTVSVFKDIGAN
Sbjct: 1   MEIL---RTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGAN 60

Query: 61  AGVISGFLYSAVTPASRR------SSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPP 120
           AGV SG LY+  T    R        + GPWVV A GA+QCF GY  IWA+V+G+I +PP
Sbjct: 61  AGVFSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPP 120

Query: 121 VPAMCFFMFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGT 180
           VP MC FMFLAA +QTFFNTANVV+ V NFA Y GT VGIMK                  
Sbjct: 121 VPLMCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMK------------------ 180

Query: 181 LDFGFDFSLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWF 240
                            G+LGLSGA+LIQ+Y T C G+P++F+L+LAV PT+LS++ M  
Sbjct: 181 -----------------GFLGLSGAILIQLYETLCAGDPASFILLLAVTPTVLSLLVMPL 240

Query: 241 VRIDKTESRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAP 300
           VRI +T   ++ KHLN LSA+++I+A YLM++IIL NTF LSSW   +T   LL++LA P
Sbjct: 241 VRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGLSSWANIVTLVCLLVMLALP 300

Query: 301 LGIAINAQREENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKP 360
           L IA  AQR+    G   ++    SP+++ PK+  + +        S+   ++ A  S  
Sbjct: 301 LLIARRAQRD----GMEKTVPHDYSPLISSPKATTSGNQ------SSEGDSKVEAGLS-- 360

Query: 361 PQAMNVLEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIW 420
            + +N+L+A++ ++FWLLFLAM+CGMGSGL+TINN+ Q+G+SL Y++ E  + VSLWSIW
Sbjct: 361 -ENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINSLVSLWSIW 420

Query: 421 NFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICY 480
           NFLGRFGAGY SD LLH  GW RPLLMA TL  MS GH++IASGF GNLYVGS++VG+CY
Sbjct: 421 NFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGSVIVGVCY 480

Query: 481 GSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSG 540
           GSQWSLMPTITSE+FG+ HMGTIFNTI++ASP+GSYIFSVR+IGYIYD+ A+G+  +C G
Sbjct: 481 GSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYDKTASGEGNTCYG 533

Query: 541 THCFVVSFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVEHR 579
           +HCF +SF++MA+VAF GFLVA  LFFRT+  YR  L +R+ HR
Sbjct: 541 SHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQILVKRLHHR 533

BLAST of Lag0034174 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 563.1 bits (1450), Expect = 4.3e-160
Identity = 299/576 (51.91%), Postives = 393/576 (68.23%), Query Frame = 0

Query: 7   ITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGANAGVISG 66
           +  KW+A    IWIQC  G SYTF IYS+ LKSTQ YDQSTLDTVSVFKDIG N GV+SG
Sbjct: 6   LRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVLSG 65

Query: 67  FLYSAVTPASRRSSS----AGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCFFM 126
            +Y+A T   RR        GPWVV   GA+  F GY  +WA+V+G+I RPPVP MC FM
Sbjct: 66  LVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCLFM 125

Query: 127 FLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFDFS 186
           F+AA + TF NTANVV+ + NFA Y GT VGIMK                          
Sbjct: 126 FIAAQSLTFLNTANVVSSLENFADYGGTAVGIMK-------------------------- 185

Query: 187 LDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKTES 246
                    G++GLSGA+LIQ+Y   C G+P  F+L+LA++P+LLSV+ M  VR+ KT +
Sbjct: 186 ---------GFVGLSGAMLIQLYEVVCPGDPKTFILLLAIVPSLLSVLVMPLVRVYKTST 245

Query: 247 RNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAINAQ 306
            +E KHL+ LS L++I+A YLM+ IIL +T +L SW   +T ++LL+LL++PL +A+ A 
Sbjct: 246 VDEKKHLDGLSTLSLIIAAYLMITIILKSTLSLPSWANAVTLAVLLVLLSSPLLVAVRAH 305

Query: 307 REENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNVLE 366
           R+         L +  SP+++   ++ A  S E   L  D+S             +N+L+
Sbjct: 306 RD----SIEKPLSSVYSPLVD---NLEATTSGEILMLDEDKS-------------LNLLQ 365

Query: 367 AIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRFGA 426
           A+  V+FWLLFLAM+CGMGSG++TINN+ Q+G+SL YT+ E  + ++LW+IWNF+GRFG 
Sbjct: 366 AMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLRYTSVEINSLLALWNIWNFIGRFGG 425

Query: 427 GYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSLMP 486
           GY SD+LLH  GW RPLLMA TL  M+ GH++IASGF GNLY GSI+VGICYGSQWSLMP
Sbjct: 426 GYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIASGFQGNLYPGSIIVGICYGSQWSLMP 485

Query: 487 TITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVVSF 546
           TITSE+FG++HMGTI+NTI+IASP+GSYIFSVR+IGYIYDR   G+  +C G HCF +++
Sbjct: 486 TITSELFGVKHMGTIYNTISIASPMGSYIFSVRLIGYIYDRTIIGEGNTCYGPHCFRLAY 526

Query: 547 LVMAAVAFLGFLVAAALFFRTRRFYRLALQRRVEHR 579
           +V+A+VAFLGFLV+  L FRT+  YR   ++ +  R
Sbjct: 546 VVIASVAFLGFLVSCVLVFRTKTIYRQIFEKILHRR 526

BLAST of Lag0034174 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 557.4 bits (1435), Expect = 2.4e-158
Identity = 309/575 (53.74%), Postives = 384/575 (66.78%), Query Frame = 0

Query: 6   FITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGANAGVIS 65
           F   KW+A A  IWIQ   GASYTF IYSS LKS+Q YDQSTLDTVSV+KDIGAN G++S
Sbjct: 3   FANTKWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILS 62

Query: 66  GFLYSAVTPASRRSSS----AGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCFF 125
           G  Y+AV  ASR+S +    +GPW+V   G +Q F+GY FIW A SGVI RPPV  MC F
Sbjct: 63  GLFYTAV--ASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLF 122

Query: 126 MFLAAHAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFDF 185
           MF A H Q FFNTA VVT V NF+ Y GT VGIMK                         
Sbjct: 123 MFFAGHCQPFFNTAIVVTAVRNFSDYGGTAVGIMK------------------------- 182

Query: 186 SLDCWGNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKTE 245
                     GYLGLSGA+L+Q+Y+  C G+P N++L+LAV+P+LL +  M FVR   T 
Sbjct: 183 ----------GYLGLSGAILVQMYHIFCGGDPRNYILLLAVVPSLLILTLMPFVRTYDTV 242

Query: 246 SRNEMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAINA 305
              + KHLN LSA+++I+  YLMVVI++ N   +S   +  +F+ LL+LLA+PL +A+ A
Sbjct: 243 IAGDKKHLNGLSAISLIIVTYLMVVILVENIIGMSMPMKICSFTFLLLLLASPLLVAVRA 302

Query: 306 QREENFRGSSPSL-FTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNV 365
           QREE  R  S     T  + +L+ PK            L S    +    N      MNV
Sbjct: 303 QREEEHRFLSLDFPVTERTTLLDSPK------------LNSSSDVKDVMTND-----MNV 362

Query: 366 LEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRF 425
           LEAI T NFWLLF+AM+CGMGSGLATINN+ Q+G+SL Y+  +  + VSLWSIWNFLGRF
Sbjct: 363 LEAICTTNFWLLFVAMICGMGSGLATINNIRQMGESLRYSTVQLNSLVSLWSIWNFLGRF 422

Query: 426 GAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSL 485
           G+GY SD  LHS+GW RP+ MAITL +M+ GHIV+ASG  G+LY+GS+LVG+ YGSQWSL
Sbjct: 423 GSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVMASGLLGSLYIGSLLVGLAYGSQWSL 482

Query: 486 MPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVV 545
           MPTITSEIFG+ HMGTIF TI+IASP+GSY FSV+VIGY+YD+ A+    SC G HCF  
Sbjct: 483 MPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVKVIGYLYDKVASEDDHSCYGNHCFRT 523

Query: 546 SFLVMAAVAFLGFLVAAALFFRTRRFYRLALQRRV 576
           SFL+MAA+A LG LVA  L  RT++FY   + +R+
Sbjct: 543 SFLIMAAMALLGSLVALVLLLRTKKFYATLVAKRI 523

BLAST of Lag0034174 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 540.8 bits (1392), Expect = 2.3e-153
Identity = 293/570 (51.40%), Postives = 385/570 (67.54%), Query Frame = 0

Query: 7   ITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGANAGVISG 66
           I  KW+A A  IWIQ   GA+YTF+IYSS LKS+Q YDQSTLD VSVFKDIG   G+ISG
Sbjct: 4   INTKWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISG 63

Query: 67  FLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAA 126
           FLY+A+T  SR     GPWVV   G VQ F+G+ FIWA+V G+I  PPVP MC F+FLA 
Sbjct: 64  FLYTAMTSKSR--GFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAG 123

Query: 127 HAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFDFSLDCW 186
           H+  FFNTANVVT   NF+ Y GT VGIM+                              
Sbjct: 124 HSLPFFNTANVVTAARNFSQYGGTAVGIMQ------------------------------ 183

Query: 187 GNLILGYLGLSGALLIQVYNTACNGE--PSNFLLMLAVLPTLLSVMFMWFVRIDKTESRN 246
                G+LGLSGA+LIQ+Y+  C GE  P+ F+L+LA++PTL+  + M FVR+ +T + +
Sbjct: 184 -----GFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVPTLVMFLAMPFVRVYETVTIS 243

Query: 247 EMKHLNSLSALAVIVAFYLMVVIILNNTFTLSSWTRFITFSILLILLAAPLGIAINAQRE 306
           + KHL+ LSA+++I+A YLMVVI + N   LS   +  +F ++L+LLA+PL +A+ A RE
Sbjct: 244 DKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQIFSFILVLLLLASPLLVAVRALRE 303

Query: 307 ENFRGSSPSLFTGESPVLNKPKSVNAEDSVEYHELPSDESQRMAAANSKPPQAMNVLEAI 366
           +       +L + + PVL+    ++   S  +      +   + A +S      N+LEA+
Sbjct: 304 KR-----QTLSSLDGPVLDTSALLDPPSSNIF-----PDGDHLVAEDS------NILEAM 363

Query: 367 RTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVSLWSIWNFLGRFGAGY 426
            TVNFWLLFLAM+CGMGSG AT+NNM Q+G+SL Y++ +  + VSLWSIWNFLGRFGAGY
Sbjct: 364 STVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNSLVSLWSIWNFLGRFGAGY 423

Query: 427 TSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSILVGICYGSQWSLMPTI 486
            SD  LH + W RP+ MAITL +M+ GHI++ASG  G+LY GS+L+G+ YGSQWSLMPTI
Sbjct: 424 VSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAGSVLIGMAYGSQWSLMPTI 483

Query: 487 TSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGKHGSCSGTHCFVVSFLV 546
           TSEIFG+ HMGTI+ TI+IA P+GSYI SV+VIGY YD+ A+    SC G+ CF  SF++
Sbjct: 484 TSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKVASEDDNSCFGSQCFRTSFMI 520

Query: 547 MAAVAFLGFLVAAALFFRTRRFYRLALQRR 575
           MA+VA  G LVA+ LFFRT +FY+  + +R
Sbjct: 544 MASVALFGSLVASVLFFRTHKFYKNLVAKR 520

BLAST of Lag0034174 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 329.7 bits (844), Expect = 8.0e-90
Identity = 211/622 (33.92%), Postives = 336/622 (54.02%), Query Frame = 0

Query: 7   ITNKWIATALGIWIQCICGASYTFSIYSSALKSTQGYDQSTLDTVSVFKDIGANAGVISG 66
           +T +W      + I    GA+Y F IYS  +K T GYDQ+TL+ +S FKD+GAN GV++G
Sbjct: 18  LTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAG 77

Query: 67  FLYSAVTPASRRSSSAGPWVVHAAGAVQCFLGYIFIWAAVSGVIDRPPVPAMCFFMFLAA 126
            L + VTP         PW +   GA+  F GY  IW AV+  I +P V  MC ++ + A
Sbjct: 78  LL-NEVTP---------PWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGA 137

Query: 127 HAQTFFNTANVVTGVHNFAHYSGTIVGIMKVPTQTLSFLDYTDCCVGTLDFGFDFSLDCW 186
           ++Q+F NT ++VT V NF    G ++GI+K                              
Sbjct: 138 NSQSFANTGSLVTCVKNFPESRGVVLGILK------------------------------ 197

Query: 187 GNLILGYLGLSGALLIQVYNTACNGEPSNFLLMLAVLPTLLSVMFMWFVRIDKTESR-NE 246
                GY+GLSGA++ Q+Y      +    +LM+  LP ++S  F+  +RI K + + NE
Sbjct: 198 -----GYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSFAFLRTIRIMKVKRQTNE 257

Query: 247 MKHLNSLSALAVIVAFYLMVVIILN--NTFTLSSWTRFITFSILLILLAAPLGIAINAQR 306
           +K   +   +++ +A +LMVVII+N  + FT S +    + +++++LL  P+ + I  ++
Sbjct: 258 LKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGG--SAAVVIVLLLLPIIVVILEEK 317

Query: 307 EENFRGSSPSLFTGESPVLNKPKSVNAE------DSVEYHELPSDESQRMA--------- 366
           +         L+  +   LN P  +N        DS E+ +   +ES+ +          
Sbjct: 318 K---------LWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEKVKTPSCW 377

Query: 367 AANSKPPQA---MNVLEAIRTVNFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETK 426
                PP+      +L+A+ +V+  +LFLA +CG+G  L  I+N+ Q+G SLGY      
Sbjct: 378 TTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGYPKRSVS 437

Query: 427 TFVSLWSIWNFLGRFGAGYTSDFLLHSYGWARPLLMAITLLIMSGGHIVIASGFSGNLYV 486
           TFVSL SIWN+ GR  +G  S+  L  Y + RPL++ + LL+   GH++IA    G LYV
Sbjct: 438 TFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYV 497

Query: 487 GSILVGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREA 546
            S+++G C+G+QW L+  I SEIFGL++  T++N  ++ASP+GSY+ +VRV GY+YD EA
Sbjct: 498 ASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGYLYDVEA 557

Query: 547 A------------GKHGSCSGTHCFVVSFLVMAAVAFLGFLVAAALFFRTRRFYRLALQR 596
                        G+  +C GT CF +SF+++AAV   G LV+  L  RT++FY+  + +
Sbjct: 558 GKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFYKSDIYK 583

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021495.10.0e+0078.99Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022972528.11.5e-27987.22protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima][more]
XP_023531919.19.6e-27987.05protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo][more]
XP_022933853.14.0e-27786.70protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
KAG6587516.19.0e-27786.70Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
F4I9E17.4e-2425.46Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q6CGU82.1e-1028.16Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28... [more]
Q96TW97.5e-0826.04Probable transporter MCH1 OS=Wickerhamomyces anomalus OX=4927 GN=MCH1 PE=3 SV=1[more]
Q6FWD42.4e-0624.00Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM ... [more]
Match NameE-valueIdentityDescription
A0A6J1IA877.2e-28087.22protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471... [more]
A0A6J1F6041.9e-27786.70protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5A7UT753.1e-26784.03Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BWC23.1e-26784.03protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 ... [more]
A0A0A0LUE81.1e-26182.38Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G00847... [more]
Match NameE-valueIdentityDescription
AT1G74780.19.5e-17655.99Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G18940.14.3e-16051.91Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34350.12.4e-15853.74Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34355.12.3e-15351.40Major facilitator superfamily protein [more]
AT2G39210.18.0e-9033.92Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 359..568
e-value: 1.1E-9
score: 40.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 228..565
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 188..298
e-value: 2.6E-20
score: 73.0
coord: 607..797
e-value: 7.9E-36
score: 123.8
coord: 10..156
e-value: 2.9E-38
score: 131.8
NoneNo IPR availablePANTHERPTHR21576:SF22F25A4.25 PROTEINcoord: 597..849
NoneNo IPR availablePANTHERPTHR21576:SF22F25A4.25 PROTEINcoord: 8..577
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 597..849
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 8..577
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 10..552
e-value: 5.86959E-110
score: 343.85

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034174.1Lag0034174.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity