Lag0034134 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034134
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein of unknown function (DUF962)
Locationchr3: 4720867 .. 4723128 (-)
RNA-Seq ExpressionLag0034134
SyntenyLag0034134
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAAGACTGGATTCTTTGATCTGGAGAGGCATTTCGCCTTCCATGGCGCTTATCACTGCAACCCAGTTAACATTTTCATCCATGTCCTCTTTGTTTGGCCGATTTTCTTTACTATTCTCATGTACTTGTATTTCACCCCTTCTTTCTACACTATTCCCAAATCCTCTTCTGGCTTTGACCATGGCCTGGTTTTGAACTTCGGATTTCTTTTCACTTTAATCTATGCTGCATCTTATGTGATCTTTGATAAGAAAGCGGGGTCCATGGCCGCTTTGCTTTGTTTCGTTTGTTGGGTTGGAGCAAGCTTCATCGCTAATAGACTTGGCTATTCTCACACTTGGAAGGTAAAATCCCCTCGCTCTCTCTAATTTAGTTTCTGGGTCCCTGGTTCTTTGCAGTAAGTTTTCTGTCTCCTGATATTGATGGCAATTGGCAAGGACAAGTACTATCAGATTCATCTCTTTTTTTCTTTGGCTTACCCATTTAGATCCCTAATTCCCTTGTTATTGTTAATAATCTTCCTAATGGATCGTTTTATTCAGTGGATTAAAGTTTCTTCTCTCTAATTTTTCTTGATGGGTTCTTACAATGAGTATATGCTTGTAATCTGAATCTAATCTGCTTGGAACTTGGAAGATGTGTTAAAGTTTGACATCTTAGTGGCAGCAAGTTGGGGCCATGTCAAGTGTCAACAAACACTATTAGAGAAGTTGCACTGAATAGTTAATTTTGTTAAATCAGATAGTTAGTTAATGGCATTTGTTATATCAAGGCTTATTTATAAAGCCTTGCATGGATGAATAAAACTATGCAAGTATTATTTACTCTCTTTTTTCTAATAGATTACCTCTTGCTAAAAGAATCCTTCATCTCCTTGTCAAATGACTGGAGATAATCCTAATTCTTCTTCTACTAATCAACCAACGAATCAATCGGTGATTGATCAACATGTTAACCCCTACTTCCTCCATCATTCTGATGGTACCTATTTCGTCCTCGTCTCTGATTTGCTCACAGAGTCGAACTACACCTCTTGGAGTCAAGCAATGCTTCTCACTCTGATGGTGAAGAACAAAGAAGGTTTTGTTGATGGAACTATTGAAAAACTCATGGGTGGGTTGCTAGATTCCTGGAAAGTCTGCAAAGAACACCACTTAGCATTAATATTGCTGCAAATGCAGGGCAGGACCTTTATATGCTAGGACAATTGTCGATAATGGTTGATTATGAAAAAAAATAATACTTGACTGCATACCGGTATATATAGTGATCTTCCGACATTCTTCATTAAAATCTCGACAACCAAGAAAGAGAAAGATAGAAAATGAGAAAACTTTTTTATCTCTCCTAGTTGTCAACATTTTAATGGTGGTATGAGATTAGTATGCAATCAAGTATTTTCCAAAAAACCATAGCCTCACCAAAATTTCAAGACCATTTAATCCTTGAGAATTGCAGCGTTTATATAAGTTAACAGTAACACTTGTCAATGCCTCCTAAATGAAAAACAATTTCAGCACAATACGACTGCTCGATATTTCAAACTCCTACCTAATTCTATTAATTTATGCTATATGGGTTAGGTCTAGTCTATACTCATTTTTCCGTACTGTTTCGAGAGAAAGAAAACATCCATGGAAAAATAACACGTAATACTGTCATTTAATTATGAAGGATAGGATAATGCTGAATTTTTTACTACTTGAATGATGCTAATGATTTTTATACTTCTAACTATTTGTTGATGTGGTAGGAGAAAACTTTGGCTATTGATGAGTGTCAATGTATCATTATTCTCAAAAGAGAGTTTGTTTTTAAGTTTATTTCATTTGTATTGTATATCAGGTAGTATTGGCTGCTCAGTTGTTCTGTTGGACCAATCTGTTCATAGGCCATGGAGTGTTTGAGGTTAGATAGGTTACTTCATCTCAGTAAATTCATTGCATCTTAAGCCTCTTGGTTAAATTCTCTCTTTTTGATAGGAACGGGTACCTGCTTTGTCAGACAATCTTGCGCAATCCTTTCTAATGGATCCTTTCCTTGTATTTCTGGAGGTATTCTTTCTCTGATTTTCTTATGTTCTGAGAGTTATTTGTTGGTGTGGATAATGAAGCAGCTTTGTTGTTTCCGTTGGTTGGTTGCAGGTTCTTCAAACTTTATTCAAATATGAACCATATCCAGGGTTTAGTGCAAGTGTGCAAGCAAAGATAAAAGCAGATATACAAGAGTGGAAAGAAAAGAAAGAAAAGCTATCATAG

mRNA sequence

ATGGGGAAGACTGGATTCTTTGATCTGGAGAGGCATTTCGCCTTCCATGGCGCTTATCACTGCAACCCAGTTAACATTTTCATCCATGTCCTCTTTGTTTGGCCGATTTTCTTTACTATTCTCATGTACTTGTATTTCACCCCTTCTTTCTACACTATTCCCAAATCCTCTTCTGGCTTTGACCATGGCCTGGTTTTGAACTTCGGATTTCTTTTCACTTTAATCTATGCTGCATCTTATGTGATCTTTGATAAGAAAGCGGGGTCCATGGCCGCTTTGCTTTGTTTCGTTTGTTGGGTTGGAGCAAGCTTCATCGCTAATAGACTTGGCTATTCTCACACTTGGAAGAATCCTTCATCTCCTTGTCAAATGACTGGAGATAATCCTAATTCTTCTTCTACTAATCAACCAACGAATCAATCGGTGATTGATCAACATGTTAACCCCTACTTCCTCCATCATTCTGATGGTACCTATTTCGTCCTCGTCTCTGATTTGCTCACAGAGTCGAACTACACCTCTTGGAGTCAAGCAATGCTTCTCACTCTGATGGTGAAGAACAAAGAAGGTTTTGTTGATGGAACTATTGAAAAACTCATGGGTGGGTTGCTAGATTCCTGGAAAGTAGTATTGGCTGCTCAGTTGTTCTGTTGGACCAATCTGTTCATAGGCCATGGAGTGTTTGAGGAACGGGTACCTGCTTTGTCAGACAATCTTGCGCAATCCTTTCTAATGGATCCTTTCCTTGTATTTCTGGAGGTTCTTCAAACTTTATTCAAATATGAACCATATCCAGGGTTTAGTGCAAGTGTGCAAGCAAAGATAAAAGCAGATATACAAGAGTGGAAAGAAAAGAAAGAAAAGCTATCATAG

Coding sequence (CDS)

ATGGGGAAGACTGGATTCTTTGATCTGGAGAGGCATTTCGCCTTCCATGGCGCTTATCACTGCAACCCAGTTAACATTTTCATCCATGTCCTCTTTGTTTGGCCGATTTTCTTTACTATTCTCATGTACTTGTATTTCACCCCTTCTTTCTACACTATTCCCAAATCCTCTTCTGGCTTTGACCATGGCCTGGTTTTGAACTTCGGATTTCTTTTCACTTTAATCTATGCTGCATCTTATGTGATCTTTGATAAGAAAGCGGGGTCCATGGCCGCTTTGCTTTGTTTCGTTTGTTGGGTTGGAGCAAGCTTCATCGCTAATAGACTTGGCTATTCTCACACTTGGAAGAATCCTTCATCTCCTTGTCAAATGACTGGAGATAATCCTAATTCTTCTTCTACTAATCAACCAACGAATCAATCGGTGATTGATCAACATGTTAACCCCTACTTCCTCCATCATTCTGATGGTACCTATTTCGTCCTCGTCTCTGATTTGCTCACAGAGTCGAACTACACCTCTTGGAGTCAAGCAATGCTTCTCACTCTGATGGTGAAGAACAAAGAAGGTTTTGTTGATGGAACTATTGAAAAACTCATGGGTGGGTTGCTAGATTCCTGGAAAGTAGTATTGGCTGCTCAGTTGTTCTGTTGGACCAATCTGTTCATAGGCCATGGAGTGTTTGAGGAACGGGTACCTGCTTTGTCAGACAATCTTGCGCAATCCTTTCTAATGGATCCTTTCCTTGTATTTCTGGAGGTTCTTCAAACTTTATTCAAATATGAACCATATCCAGGGTTTAGTGCAAGTGTGCAAGCAAAGATAAAAGCAGATATACAAGAGTGGAAAGAAAAGAAAGAAAAGCTATCATAG

Protein sequence

MGKTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPKSSSGFDHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNPSSPCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQAMLLTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDNLAQSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEKLS
Homology
BLAST of Lag0034134 vs. NCBI nr
Match: XP_022933403.1 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita moschata] >KAG7021523.1 SPAC16E8.02 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 331.6 bits (849), Expect = 6.6e-87
Identity = 180/290 (62.07%), Postives = 187/290 (64.48%), Query Frame = 0

Query: 1   MGKTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPKSSSGF 60
           MGKT FFDLERHFAF+GAYH NPVNIFIHVLFVWPIFFT LMYLYFTPSFYTIPKS  GF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNPSS 120
           DHGLVLNFGFLFTLIYAASYVIFDK+AGSMAALLCFVCWVGASFI+NRLGYS T      
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQT------ 120

Query: 121 PCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQAML 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 LTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDNLA 240
                                     WKVVLAAQLFCWTN FIGHGVFE+R PAL DNLA
Sbjct: 181 --------------------------WKVVLAAQLFCWTNQFIGHGVFEKRAPALLDNLA 198

Query: 241 QSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEKLS 291
           Q+FLM PF VFLEVLQTLFKYEPYPGFSASVQAKI+ADI+EWKEKKEKLS
Sbjct: 241 QAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQAKIEADIKEWKEKKEKLS 198

BLAST of Lag0034134 vs. NCBI nr
Match: XP_038880038.1 (2-hydroxy-palmitic acid dioxygenase MPO1 [Benincasa hispida])

HSP 1 Score: 327.4 bits (838), Expect = 1.2e-85
Identity = 177/290 (61.03%), Postives = 186/290 (64.14%), Query Frame = 0

Query: 1   MGKTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPKSSSGF 60
           MGK+G FDLERHFAF+GAYH NPVNIFIH+LFVWPIFFT LMYLYFTPSFYTIPKS  GF
Sbjct: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNPSS 120
           DHGLVLNFGFLFTLIYAASYV+FDKKAGSMAALLCFVCWVGASFIANRLGYS T      
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQT------ 120

Query: 121 PCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQAML 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 LTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDNLA 240
                                     WKVVLAAQLFCWTN FIGHGVFE+R PAL DNLA
Sbjct: 181 --------------------------WKVVLAAQLFCWTNQFIGHGVFEKRAPALLDNLA 198

Query: 241 QSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEKLS 291
           Q+FLM PF V LEVLQ+LFKYEPYPGFSASVQAKIKADI+EWKEKK+KLS
Sbjct: 241 QAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQAKIKADIEEWKEKKKKLS 198

BLAST of Lag0034134 vs. NCBI nr
Match: XP_023531593.1 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 326.2 bits (835), Expect = 2.8e-85
Identity = 176/287 (61.32%), Postives = 184/287 (64.11%), Query Frame = 0

Query: 1   MGKTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPKSSSGF 60
           MGKT FFDLERHFAF+GAYH NP+NIFIHVLFVWPIFFT LMYLYFTPSFYTIPKS  GF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPINIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNPSS 120
           DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFI+NRLGYS T      
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQT------ 120

Query: 121 PCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQAML 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 LTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDNLA 240
                                     WKVVLAAQLFCWTN FIGHGVFE+R PAL DNLA
Sbjct: 181 --------------------------WKVVLAAQLFCWTNQFIGHGVFEKRAPALLDNLA 195

Query: 241 QSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKE 288
           Q+FLM PF VFLEVLQTLFKYEPYPGFSASVQAKI+ADI+EWKEKK+
Sbjct: 241 QAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQAKIEADIKEWKEKKK 195

BLAST of Lag0034134 vs. NCBI nr
Match: XP_022972978.1 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita maxima])

HSP 1 Score: 323.9 bits (829), Expect = 1.4e-84
Identity = 176/290 (60.69%), Postives = 184/290 (63.45%), Query Frame = 0

Query: 1   MGKTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPKSSSGF 60
           MGKT FFDLERH+AF+GAYH NPVNIFIHVLFVWPIFFT LMYLYFTPSFYTIPKS  GF
Sbjct: 1   MGKTAFFDLERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNPSS 120
           DHGLVLNFGFLFTL YAASYVIFDKKAGSMAALLCFVCWVGASFI+NRLGYS T      
Sbjct: 61  DHGLVLNFGFLFTLTYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQT------ 120

Query: 121 PCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQAML 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 LTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDNLA 240
                                     WKVVLAAQLFCWTN  IGHGVFE+R PAL DNLA
Sbjct: 181 --------------------------WKVVLAAQLFCWTNQIIGHGVFEKRAPALLDNLA 198

Query: 241 QSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEKLS 291
           Q+FLM PF VFLEVLQTLFKYEPYPGFS+SVQAKI+ADI+EWKE KEKLS
Sbjct: 241 QAFLMAPFFVFLEVLQTLFKYEPYPGFSSSVQAKIEADIKEWKEMKEKLS 198

BLAST of Lag0034134 vs. NCBI nr
Match: XP_004138174.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W [Cucumis sativus] >KGN63680.1 hypothetical protein Csa_014278 [Cucumis sativus])

HSP 1 Score: 317.0 bits (811), Expect = 1.7e-82
Identity = 172/290 (59.31%), Postives = 183/290 (63.10%), Query Frame = 0

Query: 1   MGKTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPKSSSGF 60
           MGKTG FDLE+ FAF+GAYH NP+NIFIHVLFVWPIFFT LMYLYFTPSFYTIPKS  GF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNPSS 120
           DHGLVLNFGFLFTL+YAA YV+FDK+AGSMAALLCFVCWVGASFIAN+LGYS T      
Sbjct: 61  DHGLVLNFGFLFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASFIANKLGYSQT------ 120

Query: 121 PCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQAML 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 LTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDNLA 240
                                     WKVVLAAQLFCWTN FIGHGVFE+R PAL DNLA
Sbjct: 181 --------------------------WKVVLAAQLFCWTNQFIGHGVFEKRAPALLDNLA 198

Query: 241 QSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEKLS 291
           Q+FLM PF V LEVLQ+LFKYEPYPGFSASVQAKIKADI+EWKE KEKLS
Sbjct: 241 QAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQAKIKADIREWKETKEKLS 198

BLAST of Lag0034134 vs. ExPASy Swiss-Prot
Match: O13737 (2-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mpo1 PE=3 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 2.2e-08
Identity = 31/66 (46.97%), Postives = 40/66 (60.61%), Query Frame = 0

Query: 215 LFCWTNLFIGHGVFEERVPALSDNLAQSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAK 274
           + CW   FIGHGVFE+R PAL DNL QS  + P   FLE         P+ G+  SV +K
Sbjct: 111 VICWILQFIGHGVFEKRKPALLDNLIQSLFIAPLFAFLET-------GPFVGYYPSVVSK 169

Query: 275 IKADIQ 281
           I+A+I+
Sbjct: 171 IRANIK 169

BLAST of Lag0034134 vs. ExPASy Swiss-Prot
Match: P25338 (2-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MPO1 PE=1 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 1.7e-05
Identity = 25/63 (39.68%), Postives = 37/63 (58.73%), Query Frame = 0

Query: 218 WTNLFIGHGVFEERVPALSDNLAQSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKA 277
           W   F+GHGVFE+R PAL DNL QS ++ P+ +  E L  L       GF   ++A ++ 
Sbjct: 108 WIFQFVGHGVFEKRRPALIDNLVQSLVLAPYFIMFEFLFKL-------GFMPRLKATLEH 163

Query: 278 DIQ 281
           D++
Sbjct: 168 DLE 163

BLAST of Lag0034134 vs. ExPASy TrEMBL
Match: A0A6J1F4N0 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita moschata OX=3662 GN=LOC111440831 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 3.2e-87
Identity = 180/290 (62.07%), Postives = 187/290 (64.48%), Query Frame = 0

Query: 1   MGKTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPKSSSGF 60
           MGKT FFDLERHFAF+GAYH NPVNIFIHVLFVWPIFFT LMYLYFTPSFYTIPKS  GF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNPSS 120
           DHGLVLNFGFLFTLIYAASYVIFDK+AGSMAALLCFVCWVGASFI+NRLGYS T      
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQT------ 120

Query: 121 PCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQAML 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 LTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDNLA 240
                                     WKVVLAAQLFCWTN FIGHGVFE+R PAL DNLA
Sbjct: 181 --------------------------WKVVLAAQLFCWTNQFIGHGVFEKRAPALLDNLA 198

Query: 241 QSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEKLS 291
           Q+FLM PF VFLEVLQTLFKYEPYPGFSASVQAKI+ADI+EWKEKKEKLS
Sbjct: 241 QAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQAKIEADIKEWKEKKEKLS 198

BLAST of Lag0034134 vs. ExPASy TrEMBL
Match: A0A6J1IBP8 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita maxima OX=3661 GN=LOC111471498 PE=4 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 6.7e-85
Identity = 176/290 (60.69%), Postives = 184/290 (63.45%), Query Frame = 0

Query: 1   MGKTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPKSSSGF 60
           MGKT FFDLERH+AF+GAYH NPVNIFIHVLFVWPIFFT LMYLYFTPSFYTIPKS  GF
Sbjct: 1   MGKTAFFDLERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNPSS 120
           DHGLVLNFGFLFTL YAASYVIFDKKAGSMAALLCFVCWVGASFI+NRLGYS T      
Sbjct: 61  DHGLVLNFGFLFTLTYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQT------ 120

Query: 121 PCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQAML 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 LTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDNLA 240
                                     WKVVLAAQLFCWTN  IGHGVFE+R PAL DNLA
Sbjct: 181 --------------------------WKVVLAAQLFCWTNQIIGHGVFEKRAPALLDNLA 198

Query: 241 QSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEKLS 291
           Q+FLM PF VFLEVLQTLFKYEPYPGFS+SVQAKI+ADI+EWKE KEKLS
Sbjct: 241 QAFLMAPFFVFLEVLQTLFKYEPYPGFSSSVQAKIEADIKEWKEMKEKLS 198

BLAST of Lag0034134 vs. ExPASy TrEMBL
Match: A0A0A0LP54 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009840 PE=4 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 8.1e-83
Identity = 172/290 (59.31%), Postives = 183/290 (63.10%), Query Frame = 0

Query: 1   MGKTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPKSSSGF 60
           MGKTG FDLE+ FAF+GAYH NP+NIFIHVLFVWPIFFT LMYLYFTPSFYTIPKS  GF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNPSS 120
           DHGLVLNFGFLFTL+YAA YV+FDK+AGSMAALLCFVCWVGASFIAN+LGYS T      
Sbjct: 61  DHGLVLNFGFLFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASFIANKLGYSQT------ 120

Query: 121 PCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQAML 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 LTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDNLA 240
                                     WKVVLAAQLFCWTN FIGHGVFE+R PAL DNLA
Sbjct: 181 --------------------------WKVVLAAQLFCWTNQFIGHGVFEKRAPALLDNLA 198

Query: 241 QSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEKLS 291
           Q+FLM PF V LEVLQ+LFKYEPYPGFSASVQAKIKADI+EWKE KEKLS
Sbjct: 241 QAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQAKIKADIREWKETKEKLS 198

BLAST of Lag0034134 vs. ExPASy TrEMBL
Match: A0A5A7UQ13 (Putative endoplasmic reticulum membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002550 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 1.1e-82
Identity = 171/290 (58.97%), Postives = 183/290 (63.10%), Query Frame = 0

Query: 1   MGKTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPKSSSGF 60
           MGKTG FDLE+ FAF+GAYH NP+NIFIHVLFVWPIFFT LMYLYFTPSFYTIPKS  GF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNPSS 120
           DHGLVLNFGF FTL+YAA YV+FDK+AGSMAALLCFVCWVGAS+IAN+LGYS T      
Sbjct: 61  DHGLVLNFGFFFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASYIANKLGYSQT------ 120

Query: 121 PCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQAML 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 LTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDNLA 240
                                     WKVVLAAQLFCWTN FIGHGVFE+R PAL DNLA
Sbjct: 181 --------------------------WKVVLAAQLFCWTNQFIGHGVFEKRAPALLDNLA 198

Query: 241 QSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEKLS 291
           Q+FLM PF V LEVLQ+LFKYEPYPGFSASVQAKIKADI+EWKEKKEKLS
Sbjct: 241 QAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQAKIKADIREWKEKKEKLS 198

BLAST of Lag0034134 vs. ExPASy TrEMBL
Match: A0A1S3BV50 (uncharacterized endoplasmic reticulum membrane protein YGL010W OS=Cucumis melo OX=3656 GN=LOC103494003 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 1.1e-82
Identity = 171/290 (58.97%), Postives = 183/290 (63.10%), Query Frame = 0

Query: 1   MGKTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPKSSSGF 60
           MGKTG FDLE+ FAF+GAYH NP+NIFIHVLFVWPIFFT LMYLYFTPSFYTIPKS  GF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNPSS 120
           DHGLVLNFGF FTL+YAA YV+FDK+AGSMAALLCFVCWVGAS+IAN+LGYS T      
Sbjct: 61  DHGLVLNFGFFFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASYIANKLGYSQT------ 120

Query: 121 PCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQAML 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 LTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDNLA 240
                                     WKVVLAAQLFCWTN FIGHGVFE+R PAL DNLA
Sbjct: 181 --------------------------WKVVLAAQLFCWTNQFIGHGVFEKRAPALLDNLA 198

Query: 241 QSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEKLS 291
           Q+FLM PF V LEVLQ+LFKYEPYPGFSASVQAKIKADI+EWKEKKEKLS
Sbjct: 241 QAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQAKIKADIREWKEKKEKLS 198

BLAST of Lag0034134 vs. TAIR 10
Match: AT1G18720.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 194.9 bits (494), Expect = 9.0e-50
Identity = 120/291 (41.24%), Postives = 149/291 (51.20%), Query Frame = 0

Query: 5   GFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLY-FTPSFYTIPKSSSGFDHG 64
           G FDLE+HFAF+GAYH NP+NI IH++FVWPIFF++L+ L+  TP F     S  GF   
Sbjct: 6   GLFDLEKHFAFYGAYHSNPINILIHIIFVWPIFFSVLLLLHSSTPIF---DPSQLGFSQS 65

Query: 65  LVL------NFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKN 124
           L L      N GF+F LIYA  Y+  DKK+G +AAL+CF CWVG+SF+A RLG S     
Sbjct: 66  LTLDGVLRFNVGFIFALIYALFYIGLDKKSGFVAALMCFSCWVGSSFLAVRLGSS----- 125

Query: 125 PSSPCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQ 184
                                                                       
Sbjct: 126 ------------------------------------------------------------ 185

Query: 185 AMLLTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSD 244
              L L                        KV LA+QL CWT  F+GHGVFE+R PAL D
Sbjct: 186 ---LAL------------------------KVGLASQLLCWTGQFVGHGVFEKRAPALLD 201

Query: 245 NLAQSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEK 289
           NL Q+FLM PF V LEVLQ++F YEPYPGF A V AK+++DI+E++ KK+K
Sbjct: 246 NLLQAFLMAPFFVLLEVLQSVFGYEPYPGFQARVNAKVESDIKEYRAKKQK 201

BLAST of Lag0034134 vs. TAIR 10
Match: AT1G74440.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-48
Identity = 114/290 (39.31%), Postives = 145/290 (50.00%), Query Frame = 0

Query: 3   KTGFFDLERHFAFHGAYHCNPVNIFIHVLFVWPIFFTILMYLYFTPSFYTIPK----SSS 62
           + G  DLE+HFAF+GAYH NP+NI IH LFVWP  F  L++LY TP      +     S 
Sbjct: 4   RIGLLDLEKHFAFYGAYHSNPINIIIHTLFVWPNVFATLLFLYSTPPILDHSQLGFLKSL 63

Query: 63  GFDHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFIANRLGYSHTWKNP 122
            FD  L L+ GF  T+ YA  Y+  DKK+G +AALLCF CW+G+SF+A RLG+S      
Sbjct: 64  TFDGVLRLDIGFTLTVTYAVFYICLDKKSGVLAALLCFSCWIGSSFLAARLGHS------ 123

Query: 123 SSPCQMTGDNPNSSSTNQPTNQSVIDQHVNPYFLHHSDGTYFVLVSDLLTESNYTSWSQA 182
                                                                       
Sbjct: 124 ------------------------------------------------------------ 183

Query: 183 MLLTLMVKNKEGFVDGTIEKLMGGLLDSWKVVLAAQLFCWTNLFIGHGVFEERVPALSDN 242
             LTL                        KV +A+QL CWT  F+GHG+FE+R PAL DN
Sbjct: 184 --LTL------------------------KVGVASQLLCWTGQFLGHGLFEKRAPALLDN 201

Query: 243 LAQSFLMDPFLVFLEVLQTLFKYEPYPGFSASVQAKIKADIQEWKEKKEK 289
           L Q+FLM PF V LEVLQ++F YEPYPGF A V +KI++ I+EW+EKK++
Sbjct: 244 LLQAFLMGPFFVLLEVLQSVFGYEPYPGFKARVDSKIESGIKEWREKKKQ 201

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022933403.16.6e-8762.07uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita mosch... [more]
XP_038880038.11.2e-8561.032-hydroxy-palmitic acid dioxygenase MPO1 [Benincasa hispida][more]
XP_023531593.12.8e-8561.32uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita pepo ... [more]
XP_022972978.11.4e-8460.69uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita maxim... [more]
XP_004138174.11.7e-8259.31uncharacterized endoplasmic reticulum membrane protein YGL010W [Cucumis sativus]... [more]
Match NameE-valueIdentityDescription
O137372.2e-0846.972-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 97... [more]
P253381.7e-0539.682-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATC... [more]
Match NameE-valueIdentityDescription
A0A6J1F4N03.2e-8762.07uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita mos... [more]
A0A6J1IBP86.7e-8560.69uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita max... [more]
A0A0A0LP548.1e-8359.31Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009840 PE=4 SV=1[more]
A0A5A7UQ131.1e-8258.97Putative endoplasmic reticulum membrane protein OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3BV501.1e-8258.97uncharacterized endoplasmic reticulum membrane protein YGL010W OS=Cucumis melo O... [more]
Match NameE-valueIdentityDescription
AT1G18720.19.0e-5041.24Protein of unknown function (DUF962) [more]
AT1G74440.11.3e-4839.31Protein of unknown function (DUF962) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029472Retrotransposon Copia-like, N-terminalPFAMPF14244Retrotran_gag_3coord: 153..198
e-value: 4.5E-15
score: 55.2
IPR0093052-hydroxy-palmitic acid dioxygenase Mpo1-likePFAMPF06127DUF962coord: 7..257
e-value: 1.8E-24
score: 85.7
IPR0093052-hydroxy-palmitic acid dioxygenase Mpo1-likePANTHERPTHR28026DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)coord: 1..116
IPR0093052-hydroxy-palmitic acid dioxygenase Mpo1-likePANTHERPTHR28026DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)coord: 202..289
NoneNo IPR availablePANTHERPTHR28026:SF8YGL010W-LIKE PROTEINcoord: 1..116
NoneNo IPR availablePANTHERPTHR28026:SF8YGL010W-LIKE PROTEINcoord: 202..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034134.1Lag0034134.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0110165 cellular anatomical entity