Lag0034036 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034036
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCalcium-binding protein CML15
Locationchr3: 3904952 .. 3905434 (-)
RNA-Seq ExpressionLag0034036
SyntenyLag0034036
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCACTCGCAGTTGAGCAGCTTAATCAATTACGCGACATTTTTGCACGATTCGACATGGATTCCGACGGAAGCCTCACGATTCTGGAACTAGCGGCGTTGCTCCGATCGCTCGGTCTCAAGCCCTCCGGCGACCAAATCCACGTTCTTCTCGCTAATATGGACTCGAACGGTAATGGCTCTGTGGAGTTCGACGAATTGGTGACCGCGATTATGCCAGATTTCAACGAGGAGGTAATGGTAAATCAGACGCATCTTCTGGAGGTTTTCCGTTCGTTCGATCGAGACGGAAATGGATACATTACCGCGGCGGAGCTGGCTGGATCGATGGCGAAAATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGACACGGATGGCGATGGCGTAATTAGCTTCAATGAATTTGCATCTGTAATGGCTAAATCGGCCGCTGAATTTCTAGGGCTTGCAATCTCTTGA

mRNA sequence

ATGGCTGCACTCGCAGTTGAGCAGCTTAATCAATTACGCGACATTTTTGCACGATTCGACATGGATTCCGACGGAAGCCTCACGATTCTGGAACTAGCGGCGTTGCTCCGATCGCTCGGTCTCAAGCCCTCCGGCGACCAAATCCACGTTCTTCTCGCTAATATGGACTCGAACGGTAATGGCTCTGTGGAGTTCGACGAATTGGTGACCGCGATTATGCCAGATTTCAACGAGGAGGTAATGGTAAATCAGACGCATCTTCTGGAGGTTTTCCGTTCGTTCGATCGAGACGGAAATGGATACATTACCGCGGCGGAGCTGGCTGGATCGATGGCGAAAATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGACACGGATGGCGATGGCGTAATTAGCTTCAATGAATTTGCATCTGTAATGGCTAAATCGGCCGCTGAATTTCTAGGGCTTGCAATCTCTTGA

Coding sequence (CDS)

ATGGCTGCACTCGCAGTTGAGCAGCTTAATCAATTACGCGACATTTTTGCACGATTCGACATGGATTCCGACGGAAGCCTCACGATTCTGGAACTAGCGGCGTTGCTCCGATCGCTCGGTCTCAAGCCCTCCGGCGACCAAATCCACGTTCTTCTCGCTAATATGGACTCGAACGGTAATGGCTCTGTGGAGTTCGACGAATTGGTGACCGCGATTATGCCAGATTTCAACGAGGAGGTAATGGTAAATCAGACGCATCTTCTGGAGGTTTTCCGTTCGTTCGATCGAGACGGAAATGGATACATTACCGCGGCGGAGCTGGCTGGATCGATGGCGAAAATGGGACAGCCATTGACGTACAGAGAGCTAACGGAGATGATGAAGGAAGCCGACACGGATGGCGATGGCGTAATTAGCTTCAATGAATTTGCATCTGTAATGGCTAAATCGGCCGCTGAATTTCTAGGGCTTGCAATCTCTTGA

Protein sequence

MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDELVTAIMPDFNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEMMKEADTDGDGVISFNEFASVMAKSAAEFLGLAIS
Homology
[Close]
BLAST of Lag0034036 vs. NCBI nr
Match: XP_004138337.2 (probable calcium-binding protein CML15 [Cucumis sativus] >XP_031741380.1 probable calcium-binding protein CML15 [Cucumis sativus])

HSP 1 Score: 303.5 bits (776), Expect = 1.1e-78
Identity = 158/160 (98.75%), Postives = 159/160 (99.38%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAAEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSAA+FLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 160

[Close]
BLAST of Lag0034036 vs. NCBI nr
Match: XP_008453344.1 (PREDICTED: probable calcium-binding protein CML15 [Cucumis melo] >KAA0058039.1 putative calcium-binding protein CML15 [Cucumis melo var. makuwa] >TYK28384.1 putative calcium-binding protein CML15 [Cucumis melo var. makuwa])

HSP 1 Score: 303.5 bits (776), Expect = 1.1e-78
Identity = 158/160 (98.75%), Postives = 159/160 (99.38%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTRLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAAEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSAA+FLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 160

[Close]
BLAST of Lag0034036 vs. NCBI nr
Match: KAE8652452.1 (hypothetical protein Csa_013816 [Cucumis sativus])

HSP 1 Score: 303.5 bits (776), Expect = 1.1e-78
Identity = 158/160 (98.75%), Postives = 159/160 (99.38%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN
Sbjct: 97  MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 156

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 157 GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 216

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAAEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSAA+FLGLAIS
Sbjct: 217 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 256

[Close]
BLAST of Lag0034036 vs. NCBI nr
Match: XP_038880166.1 (probable calcium-binding protein CML15 [Benincasa hispida])

HSP 1 Score: 301.6 bits (771), Expect = 4.0e-78
Identity = 157/160 (98.12%), Postives = 158/160 (98.75%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           MAAL VEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN
Sbjct: 1   MAALEVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAAEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSAA+FLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 160

[Close]
BLAST of Lag0034036 vs. NCBI nr
Match: XP_022135250.1 (probable calcium-binding protein CML15 [Momordica charantia])

HSP 1 Score: 301.2 bits (770), Expect = 5.3e-78
Identity = 156/160 (97.50%), Postives = 159/160 (99.38%), Query Frame = 0 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVL+ANMD+NGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLVANMDANGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQT LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAAEFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSA+EFLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSASEFLGLAIS 160

[Close]
BLAST of Lag0034036 vs. ExPASy Swiss-Prot
Match: Q9FZ75 (Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 1.5e-64
Identity = 121/154 (78.57%), Postives = 141/154 (91.56%), Query Frame = 0 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFD 66
           +Q+ QL+DIF RFDMD+DGSLTILELAALLRSLGLKPSGDQIHVLLA+MDSNGNG VEFD
Sbjct: 3   DQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFD 62

Query: 67  ELVTAIMPDFNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 126
           ELV  I+PD NEEV++N   LLE+F+SFDRDGNG+I+AAELAG+MAKMGQPLTY+ELTEM
Sbjct: 63  ELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEM 122

Query: 127 MKEADTDGDGVISFNEFASVMAKSAAEFLGLAIS 161
           +KEADT+GDGVISF EFAS+MAKSA ++ GL I+
Sbjct: 123 IKEADTNGDGVISFGEFASIMAKSAVDYFGLKIN 156

[Close]
BLAST of Lag0034036 vs. ExPASy Swiss-Prot
Match: Q9LI84 (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 PE=2 SV=2)

HSP 1 Score: 240.7 bits (613), Expect = 1.1e-62
Identity = 121/154 (78.57%), Postives = 136/154 (88.31%), Query Frame = 0 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFD 66
           +Q+ QL+DIFARFDMD DGSLT LELAALLRSLG+KP GDQI +LL  +D NGNGSVEFD
Sbjct: 8   DQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFD 67

Query: 67  ELVTAIMPDFNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 126
           ELV AI+PD NEEV++NQ  L+EVFRSFDRDGNG ITAAELAGSMAKMG PLTYRELTEM
Sbjct: 68  ELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEM 127

Query: 127 MKEADTDGDGVISFNEFASVMAKSAAEFLGLAIS 161
           M EAD++GDGVISFNEF+ +MAKSAA+FLGL  S
Sbjct: 128 MTEADSNGDGVISFNEFSHIMAKSAADFLGLTAS 161

[Close]
BLAST of Lag0034036 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 5.0e-55
Identity = 108/152 (71.05%), Postives = 130/152 (85.53%), Query Frame = 0 

Query: 8   QLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDE 67
           QL QLR++F RFDM+ DGSLT LELAALLRSLGL+P+GD++H LLA MD+NGNGSVEFDE
Sbjct: 22  QLKQLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGSVEFDE 81

Query: 68  LVTAIMPDFNEEV-MVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 127
           L  AI P    +  +V+Q  LLEVFR+FDRDGNG+I+AAELA SMA++GQPLT+ ELT M
Sbjct: 82  LAAAIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLTFEELTRM 141

Query: 128 MKEADTDGDGVISFNEFASVMAKSAAEFLGLA 159
           M++ADTDGDGVISF EFA+VMAKSA +FLG+A
Sbjct: 142 MRDADTDGDGVISFKEFAAVMAKSALDFLGVA 173

[Close]
BLAST of Lag0034036 vs. ExPASy Swiss-Prot
Match: Q5ZD81 (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 GN=CML12 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 1.8e-49
Identity = 103/162 (63.58%), Postives = 128/162 (79.01%), Query Frame = 0 

Query: 2   AALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKP-SGDQIHVLLANMDSNGN 61
           A L  EQL QL +IF RFD+D DGSLT LELAALLRSLGL+P +GD+IH L+A +D++GN
Sbjct: 86  ARLRGEQLRQLHEIFLRFDLDGDGSLTKLELAALLRSLGLRPAAGDEIHALIAAIDADGN 145

Query: 62  GSVEFDELVTA----IMPDFNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQ 121
           G+VEFDEL ++    I+      V V+Q  L E FR+FDRDGNG+I+AAELA SMA+MG 
Sbjct: 146 GTVEFDELASSLADLILGPCRPSVAVDQAELAEAFRAFDRDGNGFISAAELARSMARMGH 205

Query: 122 PLTYRELTEMMKEADTDGDGVISFNEFASVMAKSAAEFLGLA 159
           P+ Y ELT+MM+EADTDGDG+ISF EF ++MAKSA +FLGLA
Sbjct: 206 PICYAELTDMMREADTDGDGLISFEEFTAIMAKSALDFLGLA 247

[Close]
BLAST of Lag0034036 vs. ExPASy Swiss-Prot
Match: Q9M8U1 (Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 2.7e-37
Identity = 85/150 (56.67%), Postives = 98/150 (65.33%), Query Frame = 0 

Query: 2   AALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNG 61
           A L  EQL +LR+IF  FD + DGSLT LEL +LLRSLGLKPS DQ+  L+   D N NG
Sbjct: 11  AKLGDEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNG 70

Query: 62  SVEFDELVTAIMPDFNEEVMVNQTHLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYR 121
            VEF E V  + PD   +       L  +FR FDRDGNGYITAAELA SMAK+G  LT  
Sbjct: 71  LVEFSEFVALVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAE 130

Query: 122 ELTEMMKEADTDGDGVISFNEFASVMAKSA 152
           ELT M+KEAD DGDG I F EF   +  +A
Sbjct: 131 ELTGMIKEADRDGDGCIDFQEFVQAITSAA 159

The following BLAST results are available for this feature:
BLAST of Lag0034036 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004138337.21.1e-7898.75probable calcium-binding protein CML15 [Cucumis sativus] >XP_031741380.1 probabl... [more]
XP_008453344.11.1e-7898.75PREDICTED: probable calcium-binding protein CML15 [Cucumis melo] >KAA0058039.1 p... [more]
KAE8652452.11.1e-7898.75hypothetical protein Csa_013816 [Cucumis sativus][more]
XP_038880166.14.0e-7898.13probable calcium-binding protein CML15 [Benincasa hispida][more]
XP_022135250.15.3e-7897.50probable calcium-binding protein CML15 [Momordica charantia][more]
BLAST of Lag0034036 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FZ751.5e-6478.57Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 ... [more]
Q9LI841.1e-6278.57Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 ... [more]
Q6L5F45.0e-5571.05Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q5ZD811.8e-4963.58Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9M8U12.7e-3756.67Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 11..39
e-value: 0.046
score: 22.9
coord: 122..150
e-value: 8.4E-8
score: 41.9
coord: 86..114
e-value: 0.001
score: 28.4
coord: 47..75
e-value: 1.1
score: 17.6
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 86..147
e-value: 7.7E-15
score: 55.1
coord: 10..72
e-value: 1.9E-11
score: 44.3
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 82..117
score: 13.927603
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 43..78
score: 9.129593
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 7..42
score: 13.481276
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 118..153
score: 14.039183
IPR002048EF-hand domainCDDcd00051EFhcoord: 86..148
e-value: 8.6336E-19
score: 73.3509
IPR002048EF-hand domainCDDcd00051EFhcoord: 11..73
e-value: 4.23868E-13
score: 58.7133
NoneNo IPR availableGENE3D1.10.238.10coord: 79..156
e-value: 1.5E-23
score: 84.8
NoneNo IPR availableGENE3D1.10.238.10coord: 2..78
e-value: 1.1E-17
score: 66.6
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 3..159
NoneNo IPR availablePANTHERPTHR23050:SF351CALCIUM-BINDING PROTEIN CML15-RELATEDcoord: 3..159
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 95..107
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 56..68
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 131..143
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 20..32
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 4..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034036.1Lag0034036.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030234 enzyme regulator activity