Lag0033973 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033973
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein DETOXIFICATION
Locationchr3: 3394924 .. 3398219 (-)
RNA-Seq ExpressionLag0033973
SyntenyLag0033973
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGAGAAAGATCAGAGCTCATCAATGGAGTCACCCCTAATTCACATATCTGAAGAAGAAGTGGATTTGAATGTCAAGAGACATGTAAATGGTAACAACTTCAGAAGGAAACAAATAGCCGAGGAAGTCAAGAAGCAGCTATGGCTATCAGGGCCTCTTTTATCGGTCAGTCTTCTACAATACTGCTTGCAGATGATTTCAGTCATGTTTGTAGGTCATCTCGGCGAGTTGCCCCTCTCTGGTGCTTCAATGGCGACTTCTTTTGCATCAGTGACTGGTTTCAGCCTATTGGTGAGTTGGGTTTCTGTTTGTGTCTTGGGAAAAACTCTCATTCTTGCTAGATGTTCCAATAATGGCTTTCTTGAGAACGGTAGACCTTGTTTTTAGTCATTTTACTTACAATGCTCTCGATTTGAATGAATGTGTAGATGGGGATGGCTAGTGCTTTGGATACATTTTGTGGCCAATCTTATGGGGCAAAGCAGTATAACATGTTGGGTATTCATATGCAAAGAGCAATGTTTGTTCTGTTTCTTGTGAGCATTCCTCTTGCTGTTATTTGGGCTAACACAGGAGGAATTCTGAAATTACTTGGCCAAGATGCTGAAATTTCAGAAGAAGCTGGGAAATATGCTCTTTGTATCATACCAAGCCTTTTTGCTTATGGTCTTCTTCAATGCCTTAACAGATTCTTACAGACCCAAAACGTTGTTTTCCCAATGGTGATGAGTTCTGGAATAGTAGCTTTGTTTCACATCCTGATCTGTTGGGTTCTGATATTCAAAATCGGACTCGGAATTCGAGGAGCCGCCGTGGCGAACGCCATCTCTTATTGGCTCAATGTGTTGATACTGATGCTTTATGTTAAGTTTTCTTCTTCATGTTCAAAGTCTTGGACTGGCTTTTCAGTGCAGGCTTTTCACAACATCCCATATTTCCTTAAACTTGCAATTCCTTCAGCTTTCATGGTTTGGTAATTCTCTATTTACTATTACTACTTTTATCATAATCACAAAGATGCAGTTCTCTCACAATGTTCAAAAAATTGTTCAACAGCTTGGAAATGTGGTCTTTTGAGATGATGGTTCTTCTATCTGGGCGTCTACCAAATCCAAAATTAGAGACATCTGTGCTTTCTATTAGGTAAACTAGAGATATCAATTCTTCTGGAGGAAGTCTTATCTAGCCACCTCTAGCTCTACTAACTCATTTCTTTACCAGTCTTAATACTTCTGCAACATGTTGGATGATCCTAATTGGTTTGAGTAATGCAGGAAGGTAAATTTGCTACAACTTCTCATAAGAATCTTCATTTTTCTTTTTACCAAGAACTTACAAATGACCATTTTTACATGATGTTCTCTCTTGCCTGAATTTGGGAACCAAAAACTCTATATCTGTACAAATACAACTGAGTTCTTTTTTTTTCAATTTCATCTTGTTAGCACAAGAGTTTCAAATGAGCTAGGAGCTGGCGATCCCGCAGCAGCAAAGCTAGCAGGGTGTGTAGTTATGGCTATGGCCATTATCGGTGGGCTGCTGCTTGGAACTGTGTTGATTCTTATACGTAATGTTTGGGGCTATGCATTTAGCAATGAACAAGAAGTGGTTCAATATGTAGCAGATATGCTTCCTATGATTGCAGTCTCTGCTTCTCTCGATGGACTTCAATGTGTTCTTTCAGGTTCTTATTTTCTAAATACTTTACAATTGTGCACATTTCTTCTCCATATACAATTCATTTCATATGACATGTGGACTCTAGCTAGCTTTGTTTTCTTTCCATTTTTTTTTCAATGAAAGTCGGTTTCTTGGAAAAAACAATAATTGTGTAATAGACTGCCTTTTGCAAGTATACTATCATTGATAGACAAAGTTCTGTTTCAAAGATTGGACTCTTCTTTAATAACCATGAATGATTAAACCAACTAACATGCATTCACACTGATGTTAAGATAATATGCATTTACACTAATGTTTAGTAACGTTCTTGTTTCTTGTTTCTTGTTTTCATTTTTCATGAAACAGAAGCACTTAGTAACTATTTTTGTTTCTCTTCTCGTTTTTCAAAAAAATTTCTAAAATTGTGTAGAAATTTTGGAAATAACAAAACCAAGTTTCTTGTTTCTTGTTCCTGTTTTCCGTTTTTCCGAAACATAAATAAAAGCATTATTTTATCATTCTTTTCTTATAAAATGGACAATAATTATTTAAAAATAAATATATAACATAAAAAATTTGGATATTTAATTTTAAAATCTATAGTTTTTTCAAATTTTTAATCTAAAATTTCAGATAACAAGAAACAAAAAATGATTACCAAACATGTTTTTATTTCCTTTTCCAATAAAAAAGAAAGAAGAAGAAACAAGAAACCATTAGCAAACTCAATTTTGTTTTCTTTTATTAAACAAGCAGAAACAAAAAACATGAATGAGAAACGAGAAACAGGAATGTTATCAAACATTGGAGTTCAAAATTTTTGAGACAACCTCTACCTCTAGTGATTGTGATTAGCCCCTATCATTAATATCTACTCCCAAGCCAAGTTGTTTAGATGGCATTGTCAAGCAGAAAGACATGAATGGAAATGATTGAGACATTTTATGCCTTCTTCCATAAAAAAATAGGCAAAAAGTACCACTTGGGTCAAAAGAAATCAAATATCACCTTTAAACTCTATCTTATGCCAAAAGAAAAAACATATATATACAACAAAGTACTTACAGAATATAAAAAATTTCAAGGTAAATGTTGATAAATAGATATGCAATCAAAAGCTGGTGCATTGAAGAATCTGTTTGAATAAAATGCAGGCATTGCTAGAGGATGTGGTTGGCAGAAAATTGGTGCTTATGTTAATCTTGGATCATATTATCTTGTGGGAATCCCATCTGCAGTTTTGCTTGCCTTTGTGTTGCACCTTGGTGGAAAGGTCAAATTTACTGATTAACTGCATAATCACCATTTTACTTATGTTCTGTAATTAGTACTTGCTGATGGTAACTTTTTAACCTTTTTCCTGTTGAAAATTACCCTTAACAGGGGCTGTGGTCAGGCATCATCTGTGCACTTGCTGTCCAAACACTTTCTCTTGCTATCATTACCATTCGCACTAATTGGGAACGAGAAGTACAACTCTTAACTCAATTCTCTACTTTTCTTCATCCCCTCTGTTTTTCTTTTTGGAAGAAATTTGATATTCCTACTGCTTCCAGGCAGAGATAGCTGCAGAGCGAGTGTATGACACTATGGATTATTGGATGTCATCTCGTGATACTGGAGCTTCTTAG

mRNA sequence

ATGATGGAGAAAGATCAGAGCTCATCAATGGAGTCACCCCTAATTCACATATCTGAAGAAGAAGTGGATTTGAATGTCAAGAGACATGTAAATGGTAACAACTTCAGAAGGAAACAAATAGCCGAGGAAGTCAAGAAGCAGCTATGGCTATCAGGGCCTCTTTTATCGGTCAGTCTTCTACAATACTGCTTGCAGATGATTTCAGTCATGTTTGTAGGTCATCTCGGCGAGTTGCCCCTCTCTGGTGCTTCAATGGCGACTTCTTTTGCATCAGTGACTGGTTTCAGCCTATTGATGGGGATGGCTAGTGCTTTGGATACATTTTGTGGCCAATCTTATGGGGCAAAGCAGTATAACATGTTGGGTATTCATATGCAAAGAGCAATGTTTGTTCTGTTTCTTGTGAGCATTCCTCTTGCTGTTATTTGGGCTAACACAGGAGGAATTCTGAAATTACTTGGCCAAGATGCTGAAATTTCAGAAGAAGCTGGGAAATATGCTCTTTGTATCATACCAAGCCTTTTTGCTTATGGTCTTCTTCAATGCCTTAACAGATTCTTACAGACCCAAAACGTTGTTTTCCCAATGGTGATGAGTTCTGGAATAGTAGCTTTGTTTCACATCCTGATCTGTTGGGTTCTGATATTCAAAATCGGACTCGGAATTCGAGGAGCCGCCGTGGCGAACGCCATCTCTTATTGGCTCAATGTGTTGATACTGATGCTTTATGTTAAGTTTTCTTCTTCATGTTCAAAGTCTTGGACTGGCTTTTCAGTGCAGGCTTTTCACAACATCCCATATTTCCTTAAACTTGCAATTCCTTCAGCTTTCATGGTTTGCTTGGAAATGTGGTCTTTTGAGATGATGGTTCTTCTATCTGGGCGTCTACCAAATCCAAAATTAGAGACATCTGTGCTTTCTATTAGTCTTAATACTTCTGCAACATGTTGGATGATCCTAATTGGTTTGAGTAATGCAGGAAGCACAAGAGTTTCAAATGAGCTAGGAGCTGGCGATCCCGCAGCAGCAAAGCTAGCAGGGTGTGTAGTTATGGCTATGGCCATTATCGGTGGGCTGCTGCTTGGAACTGTGTTGATTCTTATACGTAATGTTTGGGGCTATGCATTTAGCAATGAACAAGAAGTGGTTCAATATGTAGCAGATATGCTTCCTATGATTGCAGTCTCTGCTTCTCTCGATGGACTTCAATGTGTTCTTTCAGGCATTGCTAGAGGATGTGGTTGGCAGAAAATTGGTGCTTATGTTAATCTTGGATCATATTATCTTGTGGGAATCCCATCTGCAGTTTTGCTTGCCTTTGTGTTGCACCTTGGTGGAAAGGGGCTGTGGTCAGGCATCATCTGTGCACTTGCTGTCCAAACACTTTCTCTTGCTATCATTACCATTCGCACTAATTGGGAACGAGAAGCAGAGATAGCTGCAGAGCGAGTGTATGACACTATGGATTATTGGATGTCATCTCGTGATACTGGAGCTTCTTAG

Coding sequence (CDS)

ATGATGGAGAAAGATCAGAGCTCATCAATGGAGTCACCCCTAATTCACATATCTGAAGAAGAAGTGGATTTGAATGTCAAGAGACATGTAAATGGTAACAACTTCAGAAGGAAACAAATAGCCGAGGAAGTCAAGAAGCAGCTATGGCTATCAGGGCCTCTTTTATCGGTCAGTCTTCTACAATACTGCTTGCAGATGATTTCAGTCATGTTTGTAGGTCATCTCGGCGAGTTGCCCCTCTCTGGTGCTTCAATGGCGACTTCTTTTGCATCAGTGACTGGTTTCAGCCTATTGATGGGGATGGCTAGTGCTTTGGATACATTTTGTGGCCAATCTTATGGGGCAAAGCAGTATAACATGTTGGGTATTCATATGCAAAGAGCAATGTTTGTTCTGTTTCTTGTGAGCATTCCTCTTGCTGTTATTTGGGCTAACACAGGAGGAATTCTGAAATTACTTGGCCAAGATGCTGAAATTTCAGAAGAAGCTGGGAAATATGCTCTTTGTATCATACCAAGCCTTTTTGCTTATGGTCTTCTTCAATGCCTTAACAGATTCTTACAGACCCAAAACGTTGTTTTCCCAATGGTGATGAGTTCTGGAATAGTAGCTTTGTTTCACATCCTGATCTGTTGGGTTCTGATATTCAAAATCGGACTCGGAATTCGAGGAGCCGCCGTGGCGAACGCCATCTCTTATTGGCTCAATGTGTTGATACTGATGCTTTATGTTAAGTTTTCTTCTTCATGTTCAAAGTCTTGGACTGGCTTTTCAGTGCAGGCTTTTCACAACATCCCATATTTCCTTAAACTTGCAATTCCTTCAGCTTTCATGGTTTGCTTGGAAATGTGGTCTTTTGAGATGATGGTTCTTCTATCTGGGCGTCTACCAAATCCAAAATTAGAGACATCTGTGCTTTCTATTAGTCTTAATACTTCTGCAACATGTTGGATGATCCTAATTGGTTTGAGTAATGCAGGAAGCACAAGAGTTTCAAATGAGCTAGGAGCTGGCGATCCCGCAGCAGCAAAGCTAGCAGGGTGTGTAGTTATGGCTATGGCCATTATCGGTGGGCTGCTGCTTGGAACTGTGTTGATTCTTATACGTAATGTTTGGGGCTATGCATTTAGCAATGAACAAGAAGTGGTTCAATATGTAGCAGATATGCTTCCTATGATTGCAGTCTCTGCTTCTCTCGATGGACTTCAATGTGTTCTTTCAGGCATTGCTAGAGGATGTGGTTGGCAGAAAATTGGTGCTTATGTTAATCTTGGATCATATTATCTTGTGGGAATCCCATCTGCAGTTTTGCTTGCCTTTGTGTTGCACCTTGGTGGAAAGGGGCTGTGGTCAGGCATCATCTGTGCACTTGCTGTCCAAACACTTTCTCTTGCTATCATTACCATTCGCACTAATTGGGAACGAGAAGCAGAGATAGCTGCAGAGCGAGTGTATGACACTATGGATTATTGGATGTCATCTCGTGATACTGGAGCTTCTTAG

Protein sequence

MMEKDQSSSMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQYCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNMLGIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQCLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILMLYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKLETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLLGTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAAERVYDTMDYWMSSRDTGAS
Homology
BLAST of Lag0033973 vs. NCBI nr
Match: XP_022135494.1 (protein DETOXIFICATION 16-like isoform X1 [Momordica charantia])

HSP 1 Score: 796.2 bits (2055), Expect = 1.6e-226
Identity = 409/487 (83.98%), Postives = 450/487 (92.40%), Query Frame = 0

Query: 2   MEKDQSSSMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQ 61
           ME DQSSS+ SPLIHIS E V  N    +N NNF RKQIAEEVKKQLWL+GPL+SVS+LQ
Sbjct: 1   MESDQSSSLSSPLIHISAEGVSSNGDTRLNDNNFTRKQIAEEVKKQLWLAGPLISVSILQ 60

Query: 62  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNML 121
           YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQY+ML
Sbjct: 61  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYHML 120

Query: 122 GIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQ 181
           GIH+QRAMFVL LVS+PLAVIWANTG ILK LGQDA+IS EAGKYALC+IPSLFA+GLLQ
Sbjct: 121 GIHLQRAMFVLLLVSVPLAVIWANTGEILKFLGQDAQISAEAGKYALCMIPSLFAFGLLQ 180

Query: 182 CLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILM 241
           CLNRFLQTQN+VFPM++SSGI AL HILICW+LIFK GL IRGAA+ANAISYWLNVL+L+
Sbjct: 181 CLNRFLQTQNIVFPMMVSSGIAALLHILICWILIFKTGLEIRGAALANAISYWLNVLMLI 240

Query: 242 LYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKL 301
           LYVKFSSSCSKSWTGFS+QAFHNIPYFLKLAIPSA MVCLEMWSFE++VLLSG LPNP+L
Sbjct: 241 LYVKFSSSCSKSWTGFSMQAFHNIPYFLKLAIPSALMVCLEMWSFELIVLLSGLLPNPQL 300

Query: 302 ETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLL 361
           ETSVLSISLNT+AT WMI  GLS AGSTR+SNELGAG PAAAKLAG VVM MAII GLLL
Sbjct: 301 ETSVLSISLNTAATVWMIPFGLSGAGSTRISNELGAGHPAAAKLAGRVVMTMAIIEGLLL 360

Query: 362 GTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAY 421
           GT+LILIRNVWGYA+S+EQEVV+YVA MLP+IAVS  +DGLQCVLSGIARGCGWQKIGA+
Sbjct: 361 GTILILIRNVWGYAYSDEQEVVEYVASMLPIIAVSNFIDGLQCVLSGIARGCGWQKIGAF 420

Query: 422 VNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAA 481
           VNLGSYYLVGIPSAVLLAFVLH+GG+GLW G ICAL VQTLSLAIITIR+NW++EA++A+
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIGGRGLWFGNICALVVQTLSLAIITIRSNWDQEAKLAS 480

Query: 482 ERVYDTM 489
           ERV+D +
Sbjct: 481 ERVHDAI 487

BLAST of Lag0033973 vs. NCBI nr
Match: KAG6587668.1 (Protein DETOXIFICATION 16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 778.1 bits (2008), Expect = 4.6e-221
Identity = 410/487 (84.19%), Postives = 440/487 (90.35%), Query Frame = 0

Query: 2   MEKDQSSSMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQ 61
           MEKD +SS++S L+HISE+ +D         N  RRK IAEEVKKQLWL+GPL+ VSLLQ
Sbjct: 1   MEKDSNSSLKSALLHISEDGLD--------SNGERRKLIAEEVKKQLWLAGPLILVSLLQ 60

Query: 62  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNML 121
           YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLL+GMASALDTFCGQSYGAKQY+ML
Sbjct: 61  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLVGMASALDTFCGQSYGAKQYHML 120

Query: 122 GIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQ 181
           GIHMQRAM VL LVSIPLAVIWANTG ILKLLGQD EIS EAGKYA+ +IPSLFAYGLLQ
Sbjct: 121 GIHMQRAMVVLLLVSIPLAVIWANTGEILKLLGQDPEISAEAGKYAISMIPSLFAYGLLQ 180

Query: 182 CLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILM 241
           CLNRFLQTQNVVFPMVMSSGI AL HI ICWVLIFK+GL IRGAAVAN+ISYWLNVLIL 
Sbjct: 181 CLNRFLQTQNVVFPMVMSSGIAALLHIPICWVLIFKVGLEIRGAAVANSISYWLNVLILT 240

Query: 242 LYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKL 301
           LYVKFSSSCS SWTGFSVQAFHNIPYFLKLAIPSA MVCLEMWSFEMMVLLSG LPNPKL
Sbjct: 241 LYVKFSSSCSSSWTGFSVQAFHNIPYFLKLAIPSASMVCLEMWSFEMMVLLSGLLPNPKL 300

Query: 302 ETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLL 361
           ETSVLSISLNT+AT WMI  GLS AGSTRVSNELGAG PAAAKLAG VVM +AII GLLL
Sbjct: 301 ETSVLSISLNTAATVWMIPFGLSGAGSTRVSNELGAGHPAAAKLAGKVVMTIAIIEGLLL 360

Query: 362 GTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAY 421
           G VLILIR VWGYA+SNE+EVV+YVA+MLP++A S  +DGLQCVLSGIARGCGWQKIGAY
Sbjct: 361 GIVLILIRTVWGYAYSNEREVVEYVANMLPLVAASNFVDGLQCVLSGIARGCGWQKIGAY 420

Query: 422 VNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAA 481
           VNLGSYY+VGIPSAVLLAFVLH+GG+GLW GIICAL VQ LSLAIITIRTNW++EA+IA 
Sbjct: 421 VNLGSYYIVGIPSAVLLAFVLHIGGRGLWLGIICALIVQALSLAIITIRTNWDQEAKIAT 479

Query: 482 ERVYDTM 489
           ERVYDT+
Sbjct: 481 ERVYDTV 479

BLAST of Lag0033973 vs. NCBI nr
Match: XP_023530635.1 (protein DETOXIFICATION 16-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 776.9 bits (2005), Expect = 1.0e-220
Identity = 409/487 (83.98%), Postives = 440/487 (90.35%), Query Frame = 0

Query: 2   MEKDQSSSMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQ 61
           MEKD +SS++S L+HISE+ +D         N  RRK IAEEVKKQLWL+GPL+ VSLLQ
Sbjct: 1   MEKDSNSSLKSALLHISEDGLD--------SNGERRKLIAEEVKKQLWLAGPLILVSLLQ 60

Query: 62  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNML 121
           YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLL+GMASALDTFCGQSYGAKQY+ML
Sbjct: 61  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLVGMASALDTFCGQSYGAKQYHML 120

Query: 122 GIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQ 181
           GIHMQRAM VL LVSIPLAVIWANTG IL+LLGQD EIS EAGKYA+ +IPSLFAYGLLQ
Sbjct: 121 GIHMQRAMVVLLLVSIPLAVIWANTGEILRLLGQDPEISAEAGKYAISMIPSLFAYGLLQ 180

Query: 182 CLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILM 241
           CLNRFLQTQNVVFPMVMSSGI AL HI ICWVLIFK+GL IRGAAVAN+ISYWLNVLIL 
Sbjct: 181 CLNRFLQTQNVVFPMVMSSGIAALLHIPICWVLIFKVGLEIRGAAVANSISYWLNVLILT 240

Query: 242 LYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKL 301
           LYVKFSSSCS SWTGFSVQAFHNIPYFLKLAIPSA MVCLEMWSFEMMVLLSG LPNPKL
Sbjct: 241 LYVKFSSSCSSSWTGFSVQAFHNIPYFLKLAIPSACMVCLEMWSFEMMVLLSGLLPNPKL 300

Query: 302 ETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLL 361
           ETSVLSISLNT+AT WMI  GLS AGSTRVSNELGAG PAAAKLAG VVM +AII GLLL
Sbjct: 301 ETSVLSISLNTAATVWMIPFGLSGAGSTRVSNELGAGHPAAAKLAGKVVMTIAIIEGLLL 360

Query: 362 GTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAY 421
           G VLILIR VWGYA+SNE+EVV+YVA+MLP++A S  +DGLQCVLSGIARGCGWQKIGAY
Sbjct: 361 GIVLILIRTVWGYAYSNEREVVEYVANMLPLVAASNFVDGLQCVLSGIARGCGWQKIGAY 420

Query: 422 VNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAA 481
           VNLGSYY+VGIPSAVLLAFVLH+GG+GLW GIICAL VQ LSLAIITIRTNW++EA+IA 
Sbjct: 421 VNLGSYYIVGIPSAVLLAFVLHIGGRGLWLGIICALIVQALSLAIITIRTNWDQEAKIAT 479

Query: 482 ERVYDTM 489
           ERVYDT+
Sbjct: 481 ERVYDTV 479

BLAST of Lag0033973 vs. NCBI nr
Match: XP_023005105.1 (protein DETOXIFICATION 16-like [Cucurbita maxima])

HSP 1 Score: 776.5 bits (2004), Expect = 1.3e-220
Identity = 409/487 (83.98%), Postives = 440/487 (90.35%), Query Frame = 0

Query: 2   MEKDQSSSMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQ 61
           MEKD +SS++S L+HISE+ +D         N  RRK IAEEVKKQLWL+GPL+ VSLLQ
Sbjct: 1   MEKDSNSSLKSALLHISEDGLD--------SNGERRKLIAEEVKKQLWLAGPLILVSLLQ 60

Query: 62  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNML 121
           YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLL+GMASALDTFCGQSYGAKQY+ML
Sbjct: 61  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLVGMASALDTFCGQSYGAKQYHML 120

Query: 122 GIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQ 181
           GIHMQRAM VL LVSIPLAVIWANTG ILKLLGQD EIS EAGKYA+ +IPSLFAYGLLQ
Sbjct: 121 GIHMQRAMVVLLLVSIPLAVIWANTGEILKLLGQDPEISAEAGKYAISMIPSLFAYGLLQ 180

Query: 182 CLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILM 241
           CLNRFLQTQNVVFPMVMSSGI AL HI ICWVLIFK+GL IRGAAVAN+ISYWLNVLIL 
Sbjct: 181 CLNRFLQTQNVVFPMVMSSGIAALLHIPICWVLIFKVGLEIRGAAVANSISYWLNVLILT 240

Query: 242 LYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKL 301
           LYVKFSSSCS SW+GFSVQAFHNIPYFLKLAIPSA MVCLEMWSFEMMVLLSG LPNPKL
Sbjct: 241 LYVKFSSSCSNSWSGFSVQAFHNIPYFLKLAIPSACMVCLEMWSFEMMVLLSGLLPNPKL 300

Query: 302 ETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLL 361
           ETSVLSISLNT+AT WMI  GLS AGSTRVSNELGAG PAAAKLAG VVM +AII GLLL
Sbjct: 301 ETSVLSISLNTAATVWMIPFGLSGAGSTRVSNELGAGHPAAAKLAGKVVMTIAIIEGLLL 360

Query: 362 GTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAY 421
           G VLILIR VWGYA+SNE+EVV+YVA+MLP++A S  +DGLQCVLSGIARGCGWQKIGAY
Sbjct: 361 GIVLILIRTVWGYAYSNEREVVEYVANMLPLVAASNFVDGLQCVLSGIARGCGWQKIGAY 420

Query: 422 VNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAA 481
           VNLGSYY+VGIPSAVLLAFVLH+GG+GLW GIICAL VQ LSLAIITIRTNW++EA+IA 
Sbjct: 421 VNLGSYYIVGIPSAVLLAFVLHIGGRGLWLGIICALIVQALSLAIITIRTNWDQEAKIAT 479

Query: 482 ERVYDTM 489
           ERVYDT+
Sbjct: 481 ERVYDTV 479

BLAST of Lag0033973 vs. NCBI nr
Match: XP_022925914.1 (protein DETOXIFICATION 16-like [Cucurbita moschata])

HSP 1 Score: 772.3 bits (1993), Expect = 2.5e-219
Identity = 406/487 (83.37%), Postives = 439/487 (90.14%), Query Frame = 0

Query: 2   MEKDQSSSMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQ 61
           MEKD +SS++S L+HISE+ +D         N  RRK IAEEVKKQLWL+GPL+ VSLLQ
Sbjct: 1   MEKDSNSSLKSALLHISEDGLD--------SNGERRKLIAEEVKKQLWLAGPLILVSLLQ 60

Query: 62  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNML 121
           YCLQM+SVMFVGHLGELPLSGASMAT+FASVTGFSLL+GMASALDTFCGQSYGAKQY+ML
Sbjct: 61  YCLQMLSVMFVGHLGELPLSGASMATAFASVTGFSLLVGMASALDTFCGQSYGAKQYHML 120

Query: 122 GIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQ 181
           GIHMQRAM VL LVSIPLAVIWANTG ILKLLGQD EIS EAGKYA+ +IPSLFAYGLLQ
Sbjct: 121 GIHMQRAMVVLLLVSIPLAVIWANTGEILKLLGQDPEISAEAGKYAISMIPSLFAYGLLQ 180

Query: 182 CLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILM 241
           CLNRFLQTQNVVFPMVMSSGI AL HI ICWVLIF++GL IRGAAVAN+ISYWLNVLIL 
Sbjct: 181 CLNRFLQTQNVVFPMVMSSGIAALLHIPICWVLIFEVGLEIRGAAVANSISYWLNVLILT 240

Query: 242 LYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKL 301
           LYVKFSSSCS SWTGFSVQAFHNIPYFLKLAIPSA MVCLEMWSFEMMVLLSG LPNPKL
Sbjct: 241 LYVKFSSSCSSSWTGFSVQAFHNIPYFLKLAIPSACMVCLEMWSFEMMVLLSGLLPNPKL 300

Query: 302 ETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLL 361
           ETSVLSI LNT+AT WMI  GLS AGSTRVSNELGAG PAAAKLAG VVM +AII GLLL
Sbjct: 301 ETSVLSIILNTAATVWMIPFGLSGAGSTRVSNELGAGHPAAAKLAGKVVMTIAIIEGLLL 360

Query: 362 GTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAY 421
           G VLILIR VWGYA+SNE+EVV+YVA+MLP++A S  +DGLQCVLSGIARGCGWQKIGAY
Sbjct: 361 GIVLILIRTVWGYAYSNEREVVEYVANMLPLVAASNFVDGLQCVLSGIARGCGWQKIGAY 420

Query: 422 VNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAA 481
           VNLGSYY+VGIPSAVLLAFVLH+GG+GLW GIICAL VQ LSLAIITIRTNW++EA+IA 
Sbjct: 421 VNLGSYYIVGIPSAVLLAFVLHIGGRGLWLGIICALIVQALSLAIITIRTNWDQEAKIAT 479

Query: 482 ERVYDTM 489
           ERVYDT+
Sbjct: 481 ERVYDTV 479

BLAST of Lag0033973 vs. ExPASy Swiss-Prot
Match: Q9FHB6 (Protein DETOXIFICATION 16 OS=Arabidopsis thaliana OX=3702 GN=DTX16 PE=2 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 4.5e-171
Identity = 303/448 (67.63%), Postives = 363/448 (81.03%), Query Frame = 0

Query: 37  RKQIAEEVKKQLWLSGPLLSVSLLQYCLQMISVMFVGHLGELPLSGASMATSFASVTGFS 96
           +  + EEVKKQLWLSGPL++VSLLQ+CLQ+ISVMFVGHLG LPLS AS+ATSFASVTGFS
Sbjct: 20  KSSVKEEVKKQLWLSGPLIAVSLLQFCLQVISVMFVGHLGSLPLSAASIATSFASVTGFS 79

Query: 97  LLMGMASALDTFCGQSYGAKQYNMLGIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQD 156
            LMG ASALDT CGQ+YGAK+Y MLGI MQRAMFVL L SIPL++IWANT  +L   GQ+
Sbjct: 80  FLMGTASALDTLCGQAYGAKKYGMLGIQMQRAMFVLTLASIPLSIIWANTEHLLVFFGQN 139

Query: 157 AEISEEAGKYALCIIPSLFAYGLLQCLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIF 216
             I+  AG YA  +IPS+FAYGLLQC NRFLQ QN VFP+V  SG+    H+L+CWVL+F
Sbjct: 140 KSIATLAGSYAKFMIPSIFAYGLLQCFNRFLQAQNNVFPVVFCSGVTTSLHVLLCWVLVF 199

Query: 217 KIGLGIRGAAVANAISYWLNVLILMLYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSA 276
           K GLG +GAA+AN+ISYWLNV++L  YVKFS SCS +WTGFS +A  +I  FL+LA+PSA
Sbjct: 200 KSGLGFQGAALANSISYWLNVVLLFCYVKFSPSCSLTWTGFSKEALRDILPFLRLAVPSA 259

Query: 277 FMVCLEMWSFEMMVLLSGRLPNPKLETSVLSISLNTSATCWMILIGLSNAGSTRVSNELG 336
            MVCLEMWSFE++VLLSG LPNP LETSVLSI LNTS T WMI  GLS A STR+SNELG
Sbjct: 260 LMVCLEMWSFELLVLLSGLLPNPVLETSVLSICLNTSGTMWMIPFGLSGAASTRISNELG 319

Query: 337 AGDPAAAKLAGCVVMAMAIIGGLLLGTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVS 396
           AG+P  AKLA  VV+ +A+   +++G+VLILIRN+WG A+S+E EVV YVA M+P++A+ 
Sbjct: 320 AGNPKVAKLAVRVVICIAVAESIVIGSVLILIRNIWGLAYSSELEVVSYVASMMPILALG 379

Query: 397 ASLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICA 456
             LD LQCVLSG+ARGCGWQKIGA +NLGSYYLVG+PS +LLAF  H+GG+GLW GIICA
Sbjct: 380 NFLDSLQCVLSGVARGCGWQKIGAIINLGSYYLVGVPSGLLLAFHFHVGGRGLWLGIICA 439

Query: 457 LAVQTLSLAIITIRTNWEREAEIAAERV 485
           L VQ   L ++TI TNW+ EA+ A  R+
Sbjct: 440 LVVQVFGLGLVTIFTNWDEEAKKATNRI 467

BLAST of Lag0033973 vs. ExPASy Swiss-Prot
Match: Q9C9U1 (Protein DETOXIFICATION 17 OS=Arabidopsis thaliana OX=3702 GN=DTX17 PE=2 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 2.6e-158
Identity = 283/451 (62.75%), Postives = 352/451 (78.05%), Query Frame = 0

Query: 39  QIAEEVKKQLWLSGPLLSVSLLQYCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLL 98
           ++ EEVKKQLWLS PL+ VSLLQY LQ+ISVMFVGHLG LPLS AS+ATSFASVTGF+ L
Sbjct: 20  RVKEEVKKQLWLSAPLIGVSLLQYSLQVISVMFVGHLGSLPLSAASIATSFASVTGFTFL 79

Query: 99  MGMASALDTFCGQSYGAKQYNMLGIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAE 158
           +G ASAL+T CGQ+YGAK Y  LGI MQRAMFVL ++S+PL++IWANT  IL L+ QD  
Sbjct: 80  LGTASALETLCGQAYGAKLYGKLGIQMQRAMFVLLILSVPLSIIWANTEQILVLVHQDKS 139

Query: 159 ISEEAGKYALCIIPSLFAYGLLQCLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKI 218
           I+  AG YA  +IPSLFAYGLLQC+NRFLQ QN VFP+ + SGI    H+L+CW+ + K 
Sbjct: 140 IASVAGSYAKYMIPSLFAYGLLQCINRFLQAQNNVFPVFVCSGITTCLHLLLCWLFVLKT 199

Query: 219 GLGIRGAAVANAISYWLNVLILMLYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFM 278
           GLG RGAA+A ++SYW NV++L  YVKFS SCS SWTGFS +AF  +  F K+A PSA M
Sbjct: 200 GLGYRGAALAISVSYWFNVILLSCYVKFSPSCSHSWTGFSKEAFQELYDFSKIAFPSAVM 259

Query: 279 VCLEMWSFEMMVLLSGRLPNPKLETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAG 338
           VCLE+WSFE++VL SG LPNP LETSVLSI LNTS T W I +GL  A S RVSNELGAG
Sbjct: 260 VCLELWSFELLVLASGLLPNPVLETSVLSICLNTSLTIWQISVGLGGAASIRVSNELGAG 319

Query: 339 DPAAAKLAGCVVMAMAIIGGLLLGTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSAS 398
           +P  AKLA  V++ +A+  G+++ TVL+ IR + G+AFS++ +++ Y A M+P++A    
Sbjct: 320 NPQVAKLAVYVIVGIAVAEGIVVVTVLLSIRKILGHAFSSDPKIIAYAASMIPIVACGNF 379

Query: 399 LDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALA 458
           LDGLQCVLSG+ARGCGWQKIGA VNLGSYYLVG+P  +LL F  H+GG+GLW GI+ AL+
Sbjct: 380 LDGLQCVLSGVARGCGWQKIGACVNLGSYYLVGVPLGLLLGFHFHIGGRGLWLGIVTALS 439

Query: 459 VQTLSLAIITIRTNWEREAEIAAERVYDTMD 490
           VQ L L+++TI TNW++EA+ A  RV  + D
Sbjct: 440 VQVLCLSLVTIFTNWDKEAKKATNRVGSSDD 470

BLAST of Lag0033973 vs. ExPASy Swiss-Prot
Match: F4IHU9 (Protein DETOXIFICATION 15 OS=Arabidopsis thaliana OX=3702 GN=DTX15 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 2.2e-157
Identity = 294/482 (61.00%), Postives = 361/482 (74.90%), Query Frame = 0

Query: 19  EEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQYCLQMISVMFVGHLGEL 78
           EE  D+     +     R + + EEV+KQL LSGPL++VSLLQ+CLQ+ISVMFVGHLG L
Sbjct: 3   EEREDMLSWPLIGEKEKRSRFVKEEVEKQLLLSGPLIAVSLLQFCLQIISVMFVGHLGSL 62

Query: 79  PLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNMLGIHMQRAMFVLFLVSIP 138
           PLS AS+ATSFASVTGF+ LMG ASA+DT CGQSYGAK Y MLGI MQRAM VL L+S+P
Sbjct: 63  PLSAASIATSFASVTGFTFLMGTASAMDTVCGQSYGAKMYGMLGIQMQRAMLVLTLLSVP 122

Query: 139 LAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQCLNRFLQTQNVVFPMVM 198
           L+++WANT   L   GQD  I+  +G YA  +IPS+FAYGLLQCLNRFLQ QN V P+V+
Sbjct: 123 LSIVWANTEHFLVFFGQDKSIAHLSGSYARFMIPSIFAYGLLQCLNRFLQAQNNVIPVVI 182

Query: 199 SSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILMLYVKFSSSCSKSWTGFS 258
            SG+    H++ICWVL+ K GLG RGAAVANAISYWLNV++L  YVKFS SCS +WTGFS
Sbjct: 183 CSGVTTSLHVIICWVLVLKSGLGFRGAAVANAISYWLNVILLSCYVKFSPSCSLTWTGFS 242

Query: 259 VQAFHNIPYFLKLAIPSAFMVC-LEMWSFEMMVLLSGRLPNPKLETSVLSISLNTSATCW 318
            +A  +I  F+KL IPSAFMVC LEMWSFE++VL SG LPNP LETS          T W
Sbjct: 243 KEARRDIIPFMKLVIPSAFMVCSLEMWSFELLVLSSGLLPNPVLETS-------CPRTVW 302

Query: 319 MILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLLGTVLILIRNVWGYAFS 378
           MI  GLS A STRVSNELG+G+P  AKLA  VV++ +I+  +L+GTVLILIR +WG+A+S
Sbjct: 303 MIPFGLSGAASTRVSNELGSGNPKGAKLAVRVVLSFSIVESILVGTVLILIRKIWGFAYS 362

Query: 379 NEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVL 438
           ++ EVV +VA MLP++A+  SLD  Q VLSG+ARGCGWQKIGA+VNLGSYYLVG+P  +L
Sbjct: 363 SDPEVVSHVASMLPILALGHSLDSFQTVLSGVARGCGWQKIGAFVNLGSYYLVGVPFGLL 422

Query: 439 LAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAAERVYDTMDYWMSSRDT 498
           L F  H+GG+GLW GIICAL VQ + L++IT  TNW+ E + A  R   + +    + D 
Sbjct: 423 LGFHFHVGGRGLWLGIICALIVQGVCLSLITFFTNWDEEVKKATSRAKSSSEVKEFAVDN 477

Query: 499 GA 500
           G+
Sbjct: 483 GS 477

BLAST of Lag0033973 vs. ExPASy Swiss-Prot
Match: Q9C994 (Protein DETOXIFICATION 14 OS=Arabidopsis thaliana OX=3702 GN=DTX14 PE=1 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.5e-121
Identity = 239/476 (50.21%), Postives = 322/476 (67.65%), Query Frame = 0

Query: 9   SMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQYCLQMIS 68
           S E  L+ +S+ E ++N K     + F R     E KK  +++GP+++V+   Y LQ+IS
Sbjct: 3   SAEKGLLVVSDRE-EVNKK-----DGFLR-----ETKKLSYIAGPMIAVNSSMYVLQVIS 62

Query: 69  VMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNMLGIHMQRA 128
           +M VGHLGEL LS  ++A SF SVTGFS++ G+ASAL+T CGQ+ GAKQY  LG+H    
Sbjct: 63  IMMVGHLGELFLSSTAIAVSFCSVTGFSVVFGLASALETLCGQANGAKQYEKLGVHTYTG 122

Query: 129 MFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQCLNRFLQ 188
           +  LFLV IPL+++W   G IL L+GQDA +++EAGK+A  +IP+LF Y  LQ L RF Q
Sbjct: 123 IVSLFLVCIPLSLLWTYIGDILSLIGQDAMVAQEAGKFATWLIPALFGYATLQPLVRFFQ 182

Query: 189 TQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILMLYVKFSS 248
            Q+++ P+VMSS      HI++CW L+FK GLG  GAA+A  +SYWLNV +L LY+ FSS
Sbjct: 183 AQSLILPLVMSSVSSLCIHIVLCWSLVFKFGLGSLGAAIAIGVSYWLNVTVLGLYMTFSS 242

Query: 249 SCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKLETSVLSI 308
           SCSKS    S+  F  +  F +  IPSA M+CLE WSFE +VLLSG LPNPKLE SVLS+
Sbjct: 243 SCSKSRATISMSLFEGMGEFFRFGIPSASMICLEWWSFEFLVLLSGILPNPKLEASVLSV 302

Query: 309 SLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLLGTVLILI 368
            L+T ++ + I   L  A STRV+NELGAG+P  A++A    M +  +  +++G ++   
Sbjct: 303 CLSTQSSLYQIPESLGAAASTRVANELGAGNPKQARMAVYTAMVITGVESIMVGAIVFGA 362

Query: 369 RNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAYVNLGSYY 428
           RNV+GY FS+E EVV YV  M P++++S   D L   LSG+ARG G Q IGAYVNL +YY
Sbjct: 363 RNVFGYLFSSETEVVDYVKSMAPLLSLSVIFDALHAALSGVARGSGRQDIGAYVNLAAYY 422

Query: 429 LVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAAERV 485
           L GIP+A+LLAF   + G+GLW GI     VQ + L +I I TNW+++A  A ERV
Sbjct: 423 LFGIPTAILLAFGFKMRGRGLWIGITVGSCVQAVLLGLIVILTNWKKQARKARERV 467

BLAST of Lag0033973 vs. ExPASy Swiss-Prot
Match: Q8L731 (Protein DETOXIFICATION 12 OS=Arabidopsis thaliana OX=3702 GN=DTX12 PE=2 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 2.4e-116
Identity = 216/456 (47.37%), Postives = 318/456 (69.74%), Query Frame = 0

Query: 29  HVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQYCLQMISVMFVGHLGELPLSGASMATS 88
           +V  ++ R      E+K+ ++ + P+ +V + Q+ LQ++S+M VGHLG L L+ AS+A+S
Sbjct: 20  NVTWSDLRDGSFTVELKRLIFFAAPMAAVVIAQFMLQIVSMMMVGHLGNLSLASASLASS 79

Query: 89  FASVTGFSLLMGMASALDTFCGQSYGAKQYNMLGIHMQRAMFVLFLVSIPLAVIWANTGG 148
           F +VTGFS ++G++ ALDT  GQ+YGAK Y  LG+    AMF L LV +PL++IW N   
Sbjct: 80  FCNVTGFSFIIGLSCALDTLSGQAYGAKLYRKLGVQTYTAMFCLALVCLPLSLIWFNMEK 139

Query: 149 ILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQCLNRFLQTQNVVFPMVMSSGIVALFHI 208
           +L +LGQD  I+ EAGKYA  +IP LFAY +LQ L R+ Q Q+++ P++++S +V   H+
Sbjct: 140 LLLILGQDPSIAHEAGKYATWLIPGLFAYAVLQPLTRYFQNQSLITPLLITSYVVFCIHV 199

Query: 209 LICWVLIFKIGLGIRGAAVANAISYWLNVLILMLYVKFSSSCSKSWTGFSVQAFHNIPYF 268
            +CW L++  GLG  G A+A ++S WL  + L  ++ +SS+CS++    S++ F  I  F
Sbjct: 200 PLCWFLVYNSGLGNLGGALAISLSNWLYAIFLGSFMYYSSACSETRAPLSMEIFDGIGEF 259

Query: 269 LKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKLETSVLSISLNTSATCWMILIGLSNAGS 328
            K A+PSA M+CLE WS+E+++LLSG LPNP+LETSVLS+ L T +T + I + ++ A S
Sbjct: 260 FKYALPSAAMICLEWWSYELIILLSGLLPNPQLETSVLSVCLQTISTMYSIPLAIAAAAS 319

Query: 329 TRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLLGTVLILIRNVWGYAFSNEQEVVQYVAD 388
           TR+SNELGAG+  AA +     M++A+I  L++   L++ RN++G+ FS+++E + YVA 
Sbjct: 320 TRISNELGAGNSRAAHIVVYAAMSLAVIDALIVSMSLLIGRNLFGHIFSSDKETIDYVAK 379

Query: 389 MLPMIAVSASLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHLGGKG 448
           M P++++S  LD LQ VLSGIARGCGWQ IGAY+NLG++YL GIP A  LAF +HL G G
Sbjct: 380 MAPLVSISLMLDALQGVLSGIARGCGWQHIGAYINLGAFYLWGIPIAASLAFWIHLKGVG 439

Query: 449 LWSGIICALAVQTLSLAIITIRTNWEREAEIAAERV 485
           LW GI     +QTL LA++T  TNWE +A+ A  R+
Sbjct: 440 LWIGIQAGAVLQTLLLALVTGCTNWESQADKARNRM 475

BLAST of Lag0033973 vs. ExPASy TrEMBL
Match: A0A6J1C1K8 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111007436 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 7.9e-227
Identity = 409/487 (83.98%), Postives = 450/487 (92.40%), Query Frame = 0

Query: 2   MEKDQSSSMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQ 61
           ME DQSSS+ SPLIHIS E V  N    +N NNF RKQIAEEVKKQLWL+GPL+SVS+LQ
Sbjct: 1   MESDQSSSLSSPLIHISAEGVSSNGDTRLNDNNFTRKQIAEEVKKQLWLAGPLISVSILQ 60

Query: 62  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNML 121
           YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQY+ML
Sbjct: 61  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYHML 120

Query: 122 GIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQ 181
           GIH+QRAMFVL LVS+PLAVIWANTG ILK LGQDA+IS EAGKYALC+IPSLFA+GLLQ
Sbjct: 121 GIHLQRAMFVLLLVSVPLAVIWANTGEILKFLGQDAQISAEAGKYALCMIPSLFAFGLLQ 180

Query: 182 CLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILM 241
           CLNRFLQTQN+VFPM++SSGI AL HILICW+LIFK GL IRGAA+ANAISYWLNVL+L+
Sbjct: 181 CLNRFLQTQNIVFPMMVSSGIAALLHILICWILIFKTGLEIRGAALANAISYWLNVLMLI 240

Query: 242 LYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKL 301
           LYVKFSSSCSKSWTGFS+QAFHNIPYFLKLAIPSA MVCLEMWSFE++VLLSG LPNP+L
Sbjct: 241 LYVKFSSSCSKSWTGFSMQAFHNIPYFLKLAIPSALMVCLEMWSFELIVLLSGLLPNPQL 300

Query: 302 ETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLL 361
           ETSVLSISLNT+AT WMI  GLS AGSTR+SNELGAG PAAAKLAG VVM MAII GLLL
Sbjct: 301 ETSVLSISLNTAATVWMIPFGLSGAGSTRISNELGAGHPAAAKLAGRVVMTMAIIEGLLL 360

Query: 362 GTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAY 421
           GT+LILIRNVWGYA+S+EQEVV+YVA MLP+IAVS  +DGLQCVLSGIARGCGWQKIGA+
Sbjct: 361 GTILILIRNVWGYAYSDEQEVVEYVASMLPIIAVSNFIDGLQCVLSGIARGCGWQKIGAF 420

Query: 422 VNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAA 481
           VNLGSYYLVGIPSAVLLAFVLH+GG+GLW G ICAL VQTLSLAIITIR+NW++EA++A+
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIGGRGLWFGNICALVVQTLSLAIITIRSNWDQEAKLAS 480

Query: 482 ERVYDTM 489
           ERV+D +
Sbjct: 481 ERVHDAI 487

BLAST of Lag0033973 vs. ExPASy TrEMBL
Match: A0A6J1KWI8 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498191 PE=3 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 6.5e-221
Identity = 409/487 (83.98%), Postives = 440/487 (90.35%), Query Frame = 0

Query: 2   MEKDQSSSMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQ 61
           MEKD +SS++S L+HISE+ +D         N  RRK IAEEVKKQLWL+GPL+ VSLLQ
Sbjct: 1   MEKDSNSSLKSALLHISEDGLD--------SNGERRKLIAEEVKKQLWLAGPLILVSLLQ 60

Query: 62  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNML 121
           YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLL+GMASALDTFCGQSYGAKQY+ML
Sbjct: 61  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLVGMASALDTFCGQSYGAKQYHML 120

Query: 122 GIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQ 181
           GIHMQRAM VL LVSIPLAVIWANTG ILKLLGQD EIS EAGKYA+ +IPSLFAYGLLQ
Sbjct: 121 GIHMQRAMVVLLLVSIPLAVIWANTGEILKLLGQDPEISAEAGKYAISMIPSLFAYGLLQ 180

Query: 182 CLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILM 241
           CLNRFLQTQNVVFPMVMSSGI AL HI ICWVLIFK+GL IRGAAVAN+ISYWLNVLIL 
Sbjct: 181 CLNRFLQTQNVVFPMVMSSGIAALLHIPICWVLIFKVGLEIRGAAVANSISYWLNVLILT 240

Query: 242 LYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKL 301
           LYVKFSSSCS SW+GFSVQAFHNIPYFLKLAIPSA MVCLEMWSFEMMVLLSG LPNPKL
Sbjct: 241 LYVKFSSSCSNSWSGFSVQAFHNIPYFLKLAIPSACMVCLEMWSFEMMVLLSGLLPNPKL 300

Query: 302 ETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLL 361
           ETSVLSISLNT+AT WMI  GLS AGSTRVSNELGAG PAAAKLAG VVM +AII GLLL
Sbjct: 301 ETSVLSISLNTAATVWMIPFGLSGAGSTRVSNELGAGHPAAAKLAGKVVMTIAIIEGLLL 360

Query: 362 GTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAY 421
           G VLILIR VWGYA+SNE+EVV+YVA+MLP++A S  +DGLQCVLSGIARGCGWQKIGAY
Sbjct: 361 GIVLILIRTVWGYAYSNEREVVEYVANMLPLVAASNFVDGLQCVLSGIARGCGWQKIGAY 420

Query: 422 VNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAA 481
           VNLGSYY+VGIPSAVLLAFVLH+GG+GLW GIICAL VQ LSLAIITIRTNW++EA+IA 
Sbjct: 421 VNLGSYYIVGIPSAVLLAFVLHIGGRGLWLGIICALIVQALSLAIITIRTNWDQEAKIAT 479

Query: 482 ERVYDTM 489
           ERVYDT+
Sbjct: 481 ERVYDTV 479

BLAST of Lag0033973 vs. ExPASy TrEMBL
Match: A0A6J1EDI7 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111433185 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.2e-219
Identity = 406/487 (83.37%), Postives = 439/487 (90.14%), Query Frame = 0

Query: 2   MEKDQSSSMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQ 61
           MEKD +SS++S L+HISE+ +D         N  RRK IAEEVKKQLWL+GPL+ VSLLQ
Sbjct: 1   MEKDSNSSLKSALLHISEDGLD--------SNGERRKLIAEEVKKQLWLAGPLILVSLLQ 60

Query: 62  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNML 121
           YCLQM+SVMFVGHLGELPLSGASMAT+FASVTGFSLL+GMASALDTFCGQSYGAKQY+ML
Sbjct: 61  YCLQMLSVMFVGHLGELPLSGASMATAFASVTGFSLLVGMASALDTFCGQSYGAKQYHML 120

Query: 122 GIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQ 181
           GIHMQRAM VL LVSIPLAVIWANTG ILKLLGQD EIS EAGKYA+ +IPSLFAYGLLQ
Sbjct: 121 GIHMQRAMVVLLLVSIPLAVIWANTGEILKLLGQDPEISAEAGKYAISMIPSLFAYGLLQ 180

Query: 182 CLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILM 241
           CLNRFLQTQNVVFPMVMSSGI AL HI ICWVLIF++GL IRGAAVAN+ISYWLNVLIL 
Sbjct: 181 CLNRFLQTQNVVFPMVMSSGIAALLHIPICWVLIFEVGLEIRGAAVANSISYWLNVLILT 240

Query: 242 LYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKL 301
           LYVKFSSSCS SWTGFSVQAFHNIPYFLKLAIPSA MVCLEMWSFEMMVLLSG LPNPKL
Sbjct: 241 LYVKFSSSCSSSWTGFSVQAFHNIPYFLKLAIPSACMVCLEMWSFEMMVLLSGLLPNPKL 300

Query: 302 ETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLL 361
           ETSVLSI LNT+AT WMI  GLS AGSTRVSNELGAG PAAAKLAG VVM +AII GLLL
Sbjct: 301 ETSVLSIILNTAATVWMIPFGLSGAGSTRVSNELGAGHPAAAKLAGKVVMTIAIIEGLLL 360

Query: 362 GTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAY 421
           G VLILIR VWGYA+SNE+EVV+YVA+MLP++A S  +DGLQCVLSGIARGCGWQKIGAY
Sbjct: 361 GIVLILIRTVWGYAYSNEREVVEYVANMLPLVAASNFVDGLQCVLSGIARGCGWQKIGAY 420

Query: 422 VNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAA 481
           VNLGSYY+VGIPSAVLLAFVLH+GG+GLW GIICAL VQ LSLAIITIRTNW++EA+IA 
Sbjct: 421 VNLGSYYIVGIPSAVLLAFVLHIGGRGLWLGIICALIVQALSLAIITIRTNWDQEAKIAT 479

Query: 482 ERVYDTM 489
           ERVYDT+
Sbjct: 481 ERVYDTV 479

BLAST of Lag0033973 vs. ExPASy TrEMBL
Match: A0A6J1BYX8 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111007022 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.7e-219
Identity = 401/488 (82.17%), Postives = 439/488 (89.96%), Query Frame = 0

Query: 1   MMEKDQSSSMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLL 60
           MM KDQ+SSM SPLIHISEE+            N RR QIAEE+KKQLWL+GPL+SV+LL
Sbjct: 1   MMGKDQNSSMSSPLIHISEEQ-----------ENRRRNQIAEELKKQLWLAGPLISVNLL 60

Query: 61  QYCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNM 120
           QYCLQMIS+MFVGHLGELPLSGASMA SFASVTGFS+++GMASALDTFCGQSYGAKQY+M
Sbjct: 61  QYCLQMISIMFVGHLGELPLSGASMAASFASVTGFSVMIGMASALDTFCGQSYGAKQYHM 120

Query: 121 LGIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLL 180
           LGIHMQRAMFVL LVSIPLAVIWANTG ILKLLGQDA+IS EAGKYAL +IPSLFA+ L+
Sbjct: 121 LGIHMQRAMFVLLLVSIPLAVIWANTGEILKLLGQDAKISAEAGKYALFLIPSLFAHSLV 180

Query: 181 QCLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLIL 240
           QCLNRFLQTQN+VFPM+MSSGI ALFHIL CWVLIFK GL  RGAAVAN+ISYW+N LIL
Sbjct: 181 QCLNRFLQTQNIVFPMMMSSGIAALFHILFCWVLIFKAGLETRGAAVANSISYWINALIL 240

Query: 241 MLYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPK 300
           +LYVKFSSSCSKSWTGFS QAFHNIPYFLKLAIPSA MVCLE+WSFEM+VLLSG L NPK
Sbjct: 241 ILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSAVMVCLELWSFEMVVLLSGLLSNPK 300

Query: 301 LETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLL 360
           LETSVLSISLNT  T WMI +GLS A STRVSNELGAG P  AKLA C+VM +A+I GLL
Sbjct: 301 LETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPTTAKLAACLVMTIAVIEGLL 360

Query: 361 LGTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGA 420
           LGTVLIL+RNVWGYA+SNEQEVV+YVADMLP+IA+S+ LDGLQCVLSGIARGCGWQKIGA
Sbjct: 361 LGTVLILVRNVWGYAYSNEQEVVRYVADMLPIIALSSFLDGLQCVLSGIARGCGWQKIGA 420

Query: 421 YVNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIA 480
           YVNLGSYYLVGIPSAVLLAFVLH+GGKGLW GIICAL VQTLSLAIITIRTNW++EAEIA
Sbjct: 421 YVNLGSYYLVGIPSAVLLAFVLHVGGKGLWFGIICALFVQTLSLAIITIRTNWDQEAEIA 477

Query: 481 AERVYDTM 489
            +RV+D +
Sbjct: 481 TKRVHDAI 477

BLAST of Lag0033973 vs. ExPASy TrEMBL
Match: A0A6J1KWH9 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498176 PE=3 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 1.0e-218
Identity = 396/487 (81.31%), Postives = 439/487 (90.14%), Query Frame = 0

Query: 2   MEKDQSSSMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQ 61
           ME+DQ S ++SPLIHISE+EV+L     V+ NNF RK IAEEVKKQLWL+GPL+SVS LQ
Sbjct: 1   MERDQESWLKSPLIHISEDEVNLKDGGLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 62  YCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNML 121
           YCLQMIS+MFVGHLGELPLSGASMA SF SVTGF++LMGMASALDTFCGQSYGAKQY+ML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 122 GIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQ 181
           GI+MQRAMFVL LVSIPLAVIWANTG ILKLLGQDA+IS EAGKYA C+IP LFA+GL+Q
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 182 CLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILM 241
           CLNRFLQTQN+VFPM+MSSGI AL HI +CWV+IFK GL  RGAA+AN+ISYW+N LIL+
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILI 240

Query: 242 LYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKL 301
           LYVKFSSSCS+SWTGFS QAFHNI YFLKLAIPS  MVCLE+WSFEM+VLLSG LPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNISYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 302 ETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLL 361
           ETSVLSISLNT  T WMI +GLS A STRVSNELGAG PA AKLA C+VM +A+I GLLL
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVIEGLLL 360

Query: 362 GTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAY 421
           GTVLILIRNVWGYA+SNEQEVV+YVAD++P+IAVS   DGLQCVLSGI RGCGWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIVPIIAVSTFFDGLQCVLSGIVRGCGWQKIGAY 420

Query: 422 VNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAA 481
           VNLGSYYLVGIPSAVLLAFVLH+ GKGLW GIICALAVQTLSLAIITIRTNW+ EA+IAA
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIDGKGLWLGIICALAVQTLSLAIITIRTNWDEEAKIAA 480

Query: 482 ERVYDTM 489
           ERVY+++
Sbjct: 481 ERVYESV 487

BLAST of Lag0033973 vs. TAIR 10
Match: AT5G52450.1 (MATE efflux family protein )

HSP 1 Score: 602.4 bits (1552), Expect = 3.2e-172
Identity = 303/448 (67.63%), Postives = 363/448 (81.03%), Query Frame = 0

Query: 37  RKQIAEEVKKQLWLSGPLLSVSLLQYCLQMISVMFVGHLGELPLSGASMATSFASVTGFS 96
           +  + EEVKKQLWLSGPL++VSLLQ+CLQ+ISVMFVGHLG LPLS AS+ATSFASVTGFS
Sbjct: 20  KSSVKEEVKKQLWLSGPLIAVSLLQFCLQVISVMFVGHLGSLPLSAASIATSFASVTGFS 79

Query: 97  LLMGMASALDTFCGQSYGAKQYNMLGIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQD 156
            LMG ASALDT CGQ+YGAK+Y MLGI MQRAMFVL L SIPL++IWANT  +L   GQ+
Sbjct: 80  FLMGTASALDTLCGQAYGAKKYGMLGIQMQRAMFVLTLASIPLSIIWANTEHLLVFFGQN 139

Query: 157 AEISEEAGKYALCIIPSLFAYGLLQCLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIF 216
             I+  AG YA  +IPS+FAYGLLQC NRFLQ QN VFP+V  SG+    H+L+CWVL+F
Sbjct: 140 KSIATLAGSYAKFMIPSIFAYGLLQCFNRFLQAQNNVFPVVFCSGVTTSLHVLLCWVLVF 199

Query: 217 KIGLGIRGAAVANAISYWLNVLILMLYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSA 276
           K GLG +GAA+AN+ISYWLNV++L  YVKFS SCS +WTGFS +A  +I  FL+LA+PSA
Sbjct: 200 KSGLGFQGAALANSISYWLNVVLLFCYVKFSPSCSLTWTGFSKEALRDILPFLRLAVPSA 259

Query: 277 FMVCLEMWSFEMMVLLSGRLPNPKLETSVLSISLNTSATCWMILIGLSNAGSTRVSNELG 336
            MVCLEMWSFE++VLLSG LPNP LETSVLSI LNTS T WMI  GLS A STR+SNELG
Sbjct: 260 LMVCLEMWSFELLVLLSGLLPNPVLETSVLSICLNTSGTMWMIPFGLSGAASTRISNELG 319

Query: 337 AGDPAAAKLAGCVVMAMAIIGGLLLGTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVS 396
           AG+P  AKLA  VV+ +A+   +++G+VLILIRN+WG A+S+E EVV YVA M+P++A+ 
Sbjct: 320 AGNPKVAKLAVRVVICIAVAESIVIGSVLILIRNIWGLAYSSELEVVSYVASMMPILALG 379

Query: 397 ASLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICA 456
             LD LQCVLSG+ARGCGWQKIGA +NLGSYYLVG+PS +LLAF  H+GG+GLW GIICA
Sbjct: 380 NFLDSLQCVLSGVARGCGWQKIGAIINLGSYYLVGVPSGLLLAFHFHVGGRGLWLGIICA 439

Query: 457 LAVQTLSLAIITIRTNWEREAEIAAERV 485
           L VQ   L ++TI TNW+ EA+ A  R+
Sbjct: 440 LVVQVFGLGLVTIFTNWDEEAKKATNRI 467

BLAST of Lag0033973 vs. TAIR 10
Match: AT1G73700.1 (MATE efflux family protein )

HSP 1 Score: 560.1 bits (1442), Expect = 1.8e-159
Identity = 283/451 (62.75%), Postives = 352/451 (78.05%), Query Frame = 0

Query: 39  QIAEEVKKQLWLSGPLLSVSLLQYCLQMISVMFVGHLGELPLSGASMATSFASVTGFSLL 98
           ++ EEVKKQLWLS PL+ VSLLQY LQ+ISVMFVGHLG LPLS AS+ATSFASVTGF+ L
Sbjct: 20  RVKEEVKKQLWLSAPLIGVSLLQYSLQVISVMFVGHLGSLPLSAASIATSFASVTGFTFL 79

Query: 99  MGMASALDTFCGQSYGAKQYNMLGIHMQRAMFVLFLVSIPLAVIWANTGGILKLLGQDAE 158
           +G ASAL+T CGQ+YGAK Y  LGI MQRAMFVL ++S+PL++IWANT  IL L+ QD  
Sbjct: 80  LGTASALETLCGQAYGAKLYGKLGIQMQRAMFVLLILSVPLSIIWANTEQILVLVHQDKS 139

Query: 159 ISEEAGKYALCIIPSLFAYGLLQCLNRFLQTQNVVFPMVMSSGIVALFHILICWVLIFKI 218
           I+  AG YA  +IPSLFAYGLLQC+NRFLQ QN VFP+ + SGI    H+L+CW+ + K 
Sbjct: 140 IASVAGSYAKYMIPSLFAYGLLQCINRFLQAQNNVFPVFVCSGITTCLHLLLCWLFVLKT 199

Query: 219 GLGIRGAAVANAISYWLNVLILMLYVKFSSSCSKSWTGFSVQAFHNIPYFLKLAIPSAFM 278
           GLG RGAA+A ++SYW NV++L  YVKFS SCS SWTGFS +AF  +  F K+A PSA M
Sbjct: 200 GLGYRGAALAISVSYWFNVILLSCYVKFSPSCSHSWTGFSKEAFQELYDFSKIAFPSAVM 259

Query: 279 VCLEMWSFEMMVLLSGRLPNPKLETSVLSISLNTSATCWMILIGLSNAGSTRVSNELGAG 338
           VCLE+WSFE++VL SG LPNP LETSVLSI LNTS T W I +GL  A S RVSNELGAG
Sbjct: 260 VCLELWSFELLVLASGLLPNPVLETSVLSICLNTSLTIWQISVGLGGAASIRVSNELGAG 319

Query: 339 DPAAAKLAGCVVMAMAIIGGLLLGTVLILIRNVWGYAFSNEQEVVQYVADMLPMIAVSAS 398
           +P  AKLA  V++ +A+  G+++ TVL+ IR + G+AFS++ +++ Y A M+P++A    
Sbjct: 320 NPQVAKLAVYVIVGIAVAEGIVVVTVLLSIRKILGHAFSSDPKIIAYAASMIPIVACGNF 379

Query: 399 LDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHLGGKGLWSGIICALA 458
           LDGLQCVLSG+ARGCGWQKIGA VNLGSYYLVG+P  +LL F  H+GG+GLW GI+ AL+
Sbjct: 380 LDGLQCVLSGVARGCGWQKIGACVNLGSYYLVGVPLGLLLGFHFHIGGRGLWLGIVTALS 439

Query: 459 VQTLSLAIITIRTNWEREAEIAAERVYDTMD 490
           VQ L L+++TI TNW++EA+ A  RV  + D
Sbjct: 440 VQVLCLSLVTIFTNWDKEAKKATNRVGSSDD 470

BLAST of Lag0033973 vs. TAIR 10
Match: AT2G34360.1 (MATE efflux family protein )

HSP 1 Score: 557.0 bits (1434), Expect = 1.6e-158
Identity = 294/482 (61.00%), Postives = 361/482 (74.90%), Query Frame = 0

Query: 19  EEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQYCLQMISVMFVGHLGEL 78
           EE  D+     +     R + + EEV+KQL LSGPL++VSLLQ+CLQ+ISVMFVGHLG L
Sbjct: 3   EEREDMLSWPLIGEKEKRSRFVKEEVEKQLLLSGPLIAVSLLQFCLQIISVMFVGHLGSL 62

Query: 79  PLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNMLGIHMQRAMFVLFLVSIP 138
           PLS AS+ATSFASVTGF+ LMG ASA+DT CGQSYGAK Y MLGI MQRAM VL L+S+P
Sbjct: 63  PLSAASIATSFASVTGFTFLMGTASAMDTVCGQSYGAKMYGMLGIQMQRAMLVLTLLSVP 122

Query: 139 LAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQCLNRFLQTQNVVFPMVM 198
           L+++WANT   L   GQD  I+  +G YA  +IPS+FAYGLLQCLNRFLQ QN V P+V+
Sbjct: 123 LSIVWANTEHFLVFFGQDKSIAHLSGSYARFMIPSIFAYGLLQCLNRFLQAQNNVIPVVI 182

Query: 199 SSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILMLYVKFSSSCSKSWTGFS 258
            SG+    H++ICWVL+ K GLG RGAAVANAISYWLNV++L  YVKFS SCS +WTGFS
Sbjct: 183 CSGVTTSLHVIICWVLVLKSGLGFRGAAVANAISYWLNVILLSCYVKFSPSCSLTWTGFS 242

Query: 259 VQAFHNIPYFLKLAIPSAFMVC-LEMWSFEMMVLLSGRLPNPKLETSVLSISLNTSATCW 318
            +A  +I  F+KL IPSAFMVC LEMWSFE++VL SG LPNP LETS          T W
Sbjct: 243 KEARRDIIPFMKLVIPSAFMVCSLEMWSFELLVLSSGLLPNPVLETS-------CPRTVW 302

Query: 319 MILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLLGTVLILIRNVWGYAFS 378
           MI  GLS A STRVSNELG+G+P  AKLA  VV++ +I+  +L+GTVLILIR +WG+A+S
Sbjct: 303 MIPFGLSGAASTRVSNELGSGNPKGAKLAVRVVLSFSIVESILVGTVLILIRKIWGFAYS 362

Query: 379 NEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVL 438
           ++ EVV +VA MLP++A+  SLD  Q VLSG+ARGCGWQKIGA+VNLGSYYLVG+P  +L
Sbjct: 363 SDPEVVSHVASMLPILALGHSLDSFQTVLSGVARGCGWQKIGAFVNLGSYYLVGVPFGLL 422

Query: 439 LAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAAERVYDTMDYWMSSRDT 498
           L F  H+GG+GLW GIICAL VQ + L++IT  TNW+ E + A  R   + +    + D 
Sbjct: 423 LGFHFHVGGRGLWLGIICALIVQGVCLSLITFFTNWDEEVKKATSRAKSSSEVKEFAVDN 477

Query: 499 GA 500
           G+
Sbjct: 483 GS 477

BLAST of Lag0033973 vs. TAIR 10
Match: AT1G71140.1 (MATE efflux family protein )

HSP 1 Score: 438.0 bits (1125), Expect = 1.1e-122
Identity = 239/476 (50.21%), Postives = 322/476 (67.65%), Query Frame = 0

Query: 9   SMESPLIHISEEEVDLNVKRHVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQYCLQMIS 68
           S E  L+ +S+ E ++N K     + F R     E KK  +++GP+++V+   Y LQ+IS
Sbjct: 3   SAEKGLLVVSDRE-EVNKK-----DGFLR-----ETKKLSYIAGPMIAVNSSMYVLQVIS 62

Query: 69  VMFVGHLGELPLSGASMATSFASVTGFSLLMGMASALDTFCGQSYGAKQYNMLGIHMQRA 128
           +M VGHLGEL LS  ++A SF SVTGFS++ G+ASAL+T CGQ+ GAKQY  LG+H    
Sbjct: 63  IMMVGHLGELFLSSTAIAVSFCSVTGFSVVFGLASALETLCGQANGAKQYEKLGVHTYTG 122

Query: 129 MFVLFLVSIPLAVIWANTGGILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQCLNRFLQ 188
           +  LFLV IPL+++W   G IL L+GQDA +++EAGK+A  +IP+LF Y  LQ L RF Q
Sbjct: 123 IVSLFLVCIPLSLLWTYIGDILSLIGQDAMVAQEAGKFATWLIPALFGYATLQPLVRFFQ 182

Query: 189 TQNVVFPMVMSSGIVALFHILICWVLIFKIGLGIRGAAVANAISYWLNVLILMLYVKFSS 248
            Q+++ P+VMSS      HI++CW L+FK GLG  GAA+A  +SYWLNV +L LY+ FSS
Sbjct: 183 AQSLILPLVMSSVSSLCIHIVLCWSLVFKFGLGSLGAAIAIGVSYWLNVTVLGLYMTFSS 242

Query: 249 SCSKSWTGFSVQAFHNIPYFLKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKLETSVLSI 308
           SCSKS    S+  F  +  F +  IPSA M+CLE WSFE +VLLSG LPNPKLE SVLS+
Sbjct: 243 SCSKSRATISMSLFEGMGEFFRFGIPSASMICLEWWSFEFLVLLSGILPNPKLEASVLSV 302

Query: 309 SLNTSATCWMILIGLSNAGSTRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLLGTVLILI 368
            L+T ++ + I   L  A STRV+NELGAG+P  A++A    M +  +  +++G ++   
Sbjct: 303 CLSTQSSLYQIPESLGAAASTRVANELGAGNPKQARMAVYTAMVITGVESIMVGAIVFGA 362

Query: 369 RNVWGYAFSNEQEVVQYVADMLPMIAVSASLDGLQCVLSGIARGCGWQKIGAYVNLGSYY 428
           RNV+GY FS+E EVV YV  M P++++S   D L   LSG+ARG G Q IGAYVNL +YY
Sbjct: 363 RNVFGYLFSSETEVVDYVKSMAPLLSLSVIFDALHAALSGVARGSGRQDIGAYVNLAAYY 422

Query: 429 LVGIPSAVLLAFVLHLGGKGLWSGIICALAVQTLSLAIITIRTNWEREAEIAAERV 485
           L GIP+A+LLAF   + G+GLW GI     VQ + L +I I TNW+++A  A ERV
Sbjct: 423 LFGIPTAILLAFGFKMRGRGLWIGITVGSCVQAVLLGLIVILTNWKKQARKARERV 467

BLAST of Lag0033973 vs. TAIR 10
Match: AT1G15170.1 (MATE efflux family protein )

HSP 1 Score: 420.6 bits (1080), Expect = 1.7e-117
Identity = 216/456 (47.37%), Postives = 318/456 (69.74%), Query Frame = 0

Query: 29  HVNGNNFRRKQIAEEVKKQLWLSGPLLSVSLLQYCLQMISVMFVGHLGELPLSGASMATS 88
           +V  ++ R      E+K+ ++ + P+ +V + Q+ LQ++S+M VGHLG L L+ AS+A+S
Sbjct: 20  NVTWSDLRDGSFTVELKRLIFFAAPMAAVVIAQFMLQIVSMMMVGHLGNLSLASASLASS 79

Query: 89  FASVTGFSLLMGMASALDTFCGQSYGAKQYNMLGIHMQRAMFVLFLVSIPLAVIWANTGG 148
           F +VTGFS ++G++ ALDT  GQ+YGAK Y  LG+    AMF L LV +PL++IW N   
Sbjct: 80  FCNVTGFSFIIGLSCALDTLSGQAYGAKLYRKLGVQTYTAMFCLALVCLPLSLIWFNMEK 139

Query: 149 ILKLLGQDAEISEEAGKYALCIIPSLFAYGLLQCLNRFLQTQNVVFPMVMSSGIVALFHI 208
           +L +LGQD  I+ EAGKYA  +IP LFAY +LQ L R+ Q Q+++ P++++S +V   H+
Sbjct: 140 LLLILGQDPSIAHEAGKYATWLIPGLFAYAVLQPLTRYFQNQSLITPLLITSYVVFCIHV 199

Query: 209 LICWVLIFKIGLGIRGAAVANAISYWLNVLILMLYVKFSSSCSKSWTGFSVQAFHNIPYF 268
            +CW L++  GLG  G A+A ++S WL  + L  ++ +SS+CS++    S++ F  I  F
Sbjct: 200 PLCWFLVYNSGLGNLGGALAISLSNWLYAIFLGSFMYYSSACSETRAPLSMEIFDGIGEF 259

Query: 269 LKLAIPSAFMVCLEMWSFEMMVLLSGRLPNPKLETSVLSISLNTSATCWMILIGLSNAGS 328
            K A+PSA M+CLE WS+E+++LLSG LPNP+LETSVLS+ L T +T + I + ++ A S
Sbjct: 260 FKYALPSAAMICLEWWSYELIILLSGLLPNPQLETSVLSVCLQTISTMYSIPLAIAAAAS 319

Query: 329 TRVSNELGAGDPAAAKLAGCVVMAMAIIGGLLLGTVLILIRNVWGYAFSNEQEVVQYVAD 388
           TR+SNELGAG+  AA +     M++A+I  L++   L++ RN++G+ FS+++E + YVA 
Sbjct: 320 TRISNELGAGNSRAAHIVVYAAMSLAVIDALIVSMSLLIGRNLFGHIFSSDKETIDYVAK 379

Query: 389 MLPMIAVSASLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHLGGKG 448
           M P++++S  LD LQ VLSGIARGCGWQ IGAY+NLG++YL GIP A  LAF +HL G G
Sbjct: 380 MAPLVSISLMLDALQGVLSGIARGCGWQHIGAYINLGAFYLWGIPIAASLAFWIHLKGVG 439

Query: 449 LWSGIICALAVQTLSLAIITIRTNWEREAEIAAERV 485
           LW GI     +QTL LA++T  TNWE +A+ A  R+
Sbjct: 440 LWIGIQAGAVLQTLLLALVTGCTNWESQADKARNRM 475

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135494.11.6e-22683.98protein DETOXIFICATION 16-like isoform X1 [Momordica charantia][more]
KAG6587668.14.6e-22184.19Protein DETOXIFICATION 16, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023530635.11.0e-22083.98protein DETOXIFICATION 16-like [Cucurbita pepo subsp. pepo][more]
XP_023005105.11.3e-22083.98protein DETOXIFICATION 16-like [Cucurbita maxima][more]
XP_022925914.12.5e-21983.37protein DETOXIFICATION 16-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FHB64.5e-17167.63Protein DETOXIFICATION 16 OS=Arabidopsis thaliana OX=3702 GN=DTX16 PE=2 SV=1[more]
Q9C9U12.6e-15862.75Protein DETOXIFICATION 17 OS=Arabidopsis thaliana OX=3702 GN=DTX17 PE=2 SV=1[more]
F4IHU92.2e-15761.00Protein DETOXIFICATION 15 OS=Arabidopsis thaliana OX=3702 GN=DTX15 PE=3 SV=1[more]
Q9C9941.5e-12150.21Protein DETOXIFICATION 14 OS=Arabidopsis thaliana OX=3702 GN=DTX14 PE=1 SV=1[more]
Q8L7312.4e-11647.37Protein DETOXIFICATION 12 OS=Arabidopsis thaliana OX=3702 GN=DTX12 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C1K87.9e-22783.98Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111007436 PE=3 SV=1[more]
A0A6J1KWI86.5e-22183.98Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498191 PE=3 SV=1[more]
A0A6J1EDI71.2e-21983.37Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111433185 PE=3 SV=1[more]
A0A6J1BYX82.7e-21982.17Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111007022 PE=3 SV=1[more]
A0A6J1KWH91.0e-21881.31Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498176 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G52450.13.2e-17267.63MATE efflux family protein [more]
AT1G73700.11.8e-15962.75MATE efflux family protein [more]
AT2G34360.11.6e-15861.00MATE efflux family protein [more]
AT1G71140.11.1e-12250.21MATE efflux family protein [more]
AT1G15170.11.7e-11747.37MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 53..213
e-value: 5.5E-37
score: 126.9
coord: 274..436
e-value: 8.1E-29
score: 100.4
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 53..450
e-value: 1.2E-95
score: 318.6
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 28..483
NoneNo IPR availablePANTHERPTHR11206:SF196PROTEIN DETOXIFICATIONcoord: 28..483
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 43..478
e-value: 0.0
score: 538.312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033973.1Lag0033973.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity