Lag0033906 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0033906 vs. NCBI nr
Match: XP_008453533.1 (PREDICTED: phospholipase A2-alpha [Cucumis melo])

HSP 1 Score: 249.2 bits (635), Expect = 2.3e-62
Identity = 119/156 (76.28%), Postives = 134/156 (85.90%), Query Frame = 0

Query: 1   MTQFQALKLALLLWLALMISVVIPSARALDIGVQALDASISMSHDCSRKCESEFCSVPPF 60
           M QF  L L LLL L L+ S+    + AL+IGVQALDASI+MS DCSRKCESEFCSVPP 
Sbjct: 1   MPQFLLLLLLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPL 60

Query: 61  LRYGKYCGLLYSGCPGESPCDGLDACCMKHDACVVAKNNDYLSHECSQSFLMCIENFKRA 120
           LRYGKYCGLLYSGCPGE PCDGLDACCMKHDACVVAKN+DYLS ECSQSFL C+ENFKR+
Sbjct: 61  LRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 120

Query: 121 KSVPFEGSKCQIHEVVDVITFVMEAALIAGRTFHNP 157
           +S PF+G+KCQ+ EV+DVI+FVMEAALIAG+ FH P
Sbjct: 121 RSRPFKGNKCQVDEVIDVISFVMEAALIAGKVFHKP 156

BLAST of Lag0033906 vs. NCBI nr
Match: XP_038878664.1 (phospholipase A2-alpha [Benincasa hispida])

HSP 1 Score: 245.0 bits (624), Expect = 4.4e-61
Identity = 111/142 (78.17%), Postives = 127/142 (89.44%), Query Frame = 0

Query: 15  LALMISVVIPSARALDIGVQALDASISMSHDCSRKCESEFCSVPPFLRYGKYCGLLYSGC 74
           L L++ +    +RAL+IGVQALDASI+MS DCSRKCESEFCSVPP LRYGKYCGLLYSGC
Sbjct: 6   LLLLLFIAFLPSRALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGC 65

Query: 75  PGESPCDGLDACCMKHDACVVAKNNDYLSHECSQSFLMCIENFKRAKSVPFEGSKCQIHE 134
           PGE PCDGLDACCMKHDACVVAKN+DYLS ECSQSF+ C+ENFKR+KS PF+G+KC++ E
Sbjct: 66  PGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFINCMENFKRSKSAPFKGNKCEVDE 125

Query: 135 VVDVITFVMEAALIAGRTFHNP 157
           V+DVI+FVMEAALIAGR FH P
Sbjct: 126 VIDVISFVMEAALIAGRVFHKP 147

BLAST of Lag0033906 vs. NCBI nr
Match: TYK28548.1 (phospholipase A2-alpha [Cucumis melo var. makuwa])

HSP 1 Score: 244.2 bits (622), Expect = 7.5e-61
Identity = 114/146 (78.08%), Postives = 129/146 (88.36%), Query Frame = 0

Query: 11  LLLWLALMISVVIPSARALDIGVQALDASISMSHDCSRKCESEFCSVPPFLRYGKYCGLL 70
           LLL L L+ S+    + AL+IGVQALDASI+MS DCSRKCESEFCSVPP LRYGKYCGLL
Sbjct: 6   LLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGKYCGLL 65

Query: 71  YSGCPGESPCDGLDACCMKHDACVVAKNNDYLSHECSQSFLMCIENFKRAKSVPFEGSKC 130
           YSGCPGE PCDGLDACCMKHDACVVAKN+DYLS ECSQSFL C+ENFKR++S PF+G+KC
Sbjct: 66  YSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSRPFKGNKC 125

Query: 131 QIHEVVDVITFVMEAALIAGRTFHNP 157
           Q+ EV+DVI+FVMEAALIAG+ FH P
Sbjct: 126 QVDEVIDVISFVMEAALIAGKVFHKP 151

BLAST of Lag0033906 vs. NCBI nr
Match: XP_022135104.1 (phospholipase A2-alpha [Momordica charantia])

HSP 1 Score: 243.8 bits (621), Expect = 9.7e-61
Identity = 117/156 (75.00%), Postives = 134/156 (85.90%), Query Frame = 0

Query: 1   MTQFQALKLALLLWLALMISVVIPSARALDIGVQALDASISMSHDCSRKCESEFCSVPPF 60
           M++FQAL   LLL  + ++ + +P ARAL+IGVQA+DASIS+S DCSRKCESEFCSVPPF
Sbjct: 1   MSRFQALLKLLLLLSSSLLCLSVP-ARALNIGVQAIDASISLSKDCSRKCESEFCSVPPF 60

Query: 61  LRYGKYCGLLYSGCPGESPCDGLDACCMKHDACVVAKNNDYLSHECSQSFLMCIENFKRA 120
           LRYGKYCGLLYSGCPGE PCDGLDACCMKHD CV AKNNDYLS ECSQSFL C+ENFKR+
Sbjct: 61  LRYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRS 120

Query: 121 KSVPFEGSKCQIHEVVDVITFVMEAALIAGRTFHNP 157
           K   F+G+KCQI EV+DVI+ VMEAALIAG+ FH P
Sbjct: 121 KQATFKGNKCQIDEVIDVISLVMEAALIAGKVFHKP 155

BLAST of Lag0033906 vs. NCBI nr
Match: XP_004150744.1 (phospholipase A2-alpha [Cucumis sativus] >KGN65513.1 hypothetical protein Csa_019839 [Cucumis sativus])

HSP 1 Score: 241.1 bits (614), Expect = 6.3e-60
Identity = 111/144 (77.08%), Postives = 127/144 (88.19%), Query Frame = 0

Query: 13  LWLALMISVVIPSARALDIGVQALDASISMSHDCSRKCESEFCSVPPFLRYGKYCGLLYS 72
           L L L+ S+V   + AL+IGVQALDAS++MS DCSRKCESEFCSVPP LRYGKYCGLLYS
Sbjct: 5   LLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYS 64

Query: 73  GCPGESPCDGLDACCMKHDACVVAKNNDYLSHECSQSFLMCIENFKRAKSVPFEGSKCQI 132
           GCPGE PCDGLDACCMKHDACVVAKN+DYLS ECSQSFL C+ENFKR++S PF+G+KCQ+
Sbjct: 65  GCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQV 124

Query: 133 HEVVDVITFVMEAALIAGRTFHNP 157
            EV++VI+ VMEAALIAGR FH P
Sbjct: 125 DEVIEVISLVMEAALIAGRVFHKP 148

BLAST of Lag0033906 vs. ExPASy Swiss-Prot
Match: Q8S8N6 (Phospholipase A2-alpha OS=Arabidopsis thaliana OX=3702 GN=PLA2-ALPHA PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 1.3e-52
Identity = 91/132 (68.94%), Postives = 107/132 (81.06%), Query Frame = 0

Query: 25  SARALDIGVQALDASISMSHDCSRKCESEFCSVPPFLRYGKYCGLLYSGCPGESPCDGLD 84
           S  AL++GVQ +  SIS++ +CSRKCESEFCSVPPFLRYGKYCGLLYSGCPGE PCDGLD
Sbjct: 17  SVSALNVGVQLIHPSISLTKECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLD 76

Query: 85  ACCMKHDACVVAKNNDYLSHECSQSFLMCIENFKRAKSVPFEGSKCQIHEVVDVITFVME 144
           +CCMKHDACV +KNNDYLS ECSQ F+ C+ NF + K   F+G+KC   EV+DVI+ VME
Sbjct: 77  SCCMKHDACVQSKNNDYLSQECSQKFINCMNNFSQKKQPTFKGNKCDADEVIDVISIVME 136

Query: 145 AALIAGRTFHNP 157
           AALIAG+    P
Sbjct: 137 AALIAGKVLKKP 148

BLAST of Lag0033906 vs. ExPASy Swiss-Prot
Match: Q10E50 (Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 3.1e-41
Identity = 79/140 (56.43%), Postives = 99/140 (70.71%), Query Frame = 0

Query: 17  LMISVVIPSARALDIGVQALDASISMSHDCSRKCESEFCSVPPFLRYGKYCGLLYSGCPG 76
           L+ +VV P A AL+IG+Q+     S +  CSR CES+ C+ PP LRYGKYCG+LYSGCPG
Sbjct: 26  LLFAVVAPPAAALNIGLQSAGDGASKAGLCSRTCESDHCTTPPLLRYGKYCGILYSGCPG 85

Query: 77  ESPCDGLDACCMKHDACVVAKNNDYLSHECSQSFLMCIENFKRAKSVPFEGSKCQIHEVV 136
           E PCD LDACCM HD CV AK NDYLS  C++  L C+   +   S  F+G+KC I EV+
Sbjct: 86  EQPCDELDACCMHHDNCVQAK-NDYLSTACNEELLECLARLREGSST-FQGNKCMIDEVI 145

Query: 137 DVITFVMEAALIAGRTFHNP 157
           DVI+ V+EAA++AGR  H P
Sbjct: 146 DVISLVIEAAVVAGRLLHKP 163

BLAST of Lag0033906 vs. ExPASy Swiss-Prot
Match: Q9XG81 (Probable phospholipase A2 homolog 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-II PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 4.7e-34
Identity = 65/138 (47.10%), Postives = 92/138 (66.67%), Query Frame = 0

Query: 17  LMISVVIPSARALDIGVQALDASISMSHDCSRKCESEFCSVPPFLRYGKYCGLLYSGCPG 76
           L++ +++ ++R L+IG   L ++ +    CSR CES+FC++ P LRYGKYCG+LYSGCPG
Sbjct: 14  LLLLLLVTASRGLNIG-DLLGSTPAKDQGCSRTCESQFCTIAPLLRYGKYCGILYSGCPG 73

Query: 77  ESPCDGLDACCMKHDACVVAKNNDYLSHECSQSFLMCIENFKRAKSVPFEGSKCQIHEVV 136
           E PCD LDACCM HD CV   N+DYL+  C+++ L CI+   R     F G+KC + +  
Sbjct: 74  ERPCDALDACCMVHDHCVDTHNDDYLNTMCNENLLSCID---RVSGATFPGNKCNVGQTA 133

Query: 137 DVITFVMEAALIAGRTFH 155
            VI  V+E A+ AG+  H
Sbjct: 134 SVIRGVIETAVFAGKILH 147

BLAST of Lag0033906 vs. ExPASy Swiss-Prot
Match: Q8GV50 (Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 7.6e-16
Identity = 41/93 (44.09%), Postives = 55/93 (59.14%), Query Frame = 0

Query: 28  ALDIGVQALDASISMSHDCSRKCESEFCSVPPFLRYGKYCGLLYSGCPGESPCDGLDACC 87
           AL + V  L A +     CS+ C ++ C+V   +RYGKYCG+ Y GCPGE PCD LD CC
Sbjct: 11  ALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLG-IRYGKYCGIGYFGCPGEPPCDDLDDCC 70

Query: 88  MKHDACVVAKNNDYLSHECSQSFLMCIENFKRA 121
           M HD CV  K   Y+  +C + F  C+   K++
Sbjct: 71  MTHDNCVDLKGMTYV--DCHKQFQRCVNELKQS 100

BLAST of Lag0033906 vs. ExPASy Swiss-Prot
Match: Q8GZB4 (Phospholipase A2-beta OS=Arabidopsis thaliana OX=3702 GN=PLA2-BETA PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 3.8e-15
Identity = 42/115 (36.52%), Postives = 62/115 (53.91%), Query Frame = 0

Query: 40  ISMSHDCSRKCESEFCSVPPFLRYGKYCGLLYSGCPGESPCDGLDACCMKHDACVVAKNN 99
           ++ S +C+R C ++ C     +RYGKYCG+ +SGCPGE PCD LDACC  HD CV  + N
Sbjct: 25  VARSEECTRTCIAQNCDTLS-IRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCV--ELN 84

Query: 100 DYLSHECSQSFLMCIENFKRA------KSVPFEGSKCQIHEVVDVITFVMEAALI 149
              +  C + F  C+    +A      K V F  +KC    V+  +   M+  ++
Sbjct: 85  GMTNISCHKKFQRCVNRLSKAIKQSKNKKVGF-STKCPYSVVIPTVNQGMDIGIL 135