Lag0033868 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0033868 vs. NCBI nr
Match: KAG6589696.1 (Cadmium/zinc-transporting ATPase HMA2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1930.6 bits (5000), Expect = 0.0e+00
Identity = 1016/1277 (79.56%), Postives = 1098/1277 (85.98%), Query Frame = 0

Query: 10   AAVAGKSGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHDNL 69
            AA A  + GG LQ+SYFDVLGICC+SEIPLIENILKE+EG+KEI+VIVATRT+IV+HDNL
Sbjct: 3    AAAAAGNNGGALQKSYFDVLGICCSSEIPLIENILKEIEGIKEIKVIVATRTLIVLHDNL 62

Query: 70   LLSQAQIVKALNQARLEANVRAYGD-QKNHRKRWPSPYAVASGLLLLLSFLKYVNPIFRW 129
            L+SQAQIVKALNQARLEANVRAYGD QKNHRK+WPSPYAVASGLLLL+SFLKYVNP+F+W
Sbjct: 63   LVSQAQIVKALNQARLEANVRAYGDQQKNHRKKWPSPYAVASGLLLLVSFLKYVNPVFKW 122

Query: 130  VALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIAEW 189
            VALAAVAAGIWPI LKSF AVR+LRIDINILALIAVIGTIV+ DYLEAATIVFLFTIAEW
Sbjct: 123  VALAAVAAGIWPIALKSFTAVRHLRIDINILALIAVIGTIVLNDYLEAATIVFLFTIAEW 182

Query: 190  LESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIVVE 249
            LESRA HKANAVMSSLLSIAPQKAVLADTGEVVGADEVK+GTLLAVKAGEDIPIDGIVVE
Sbjct: 183  LESRAGHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKMGTLLAVKAGEDIPIDGIVVE 242

Query: 250  GKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQ 309
            GKCEVDEKTLTGESFPV KQK+STVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQ
Sbjct: 243  GKCEVDEKTLTGESFPVAKQKDSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQ 302

Query: 310  NNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCALI 369
            N+KSRTQRFID CAKFYTPAVIIIST IAVIP ALRL NRSHWFHLALVVLVSACPCALI
Sbjct: 303  NSKSRTQRFIDKCAKFYTPAVIIISTGIAVIPFALRLHNRSHWFHLALVVLVSACPCALI 362

Query: 370  LSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALDKD 429
            LSTPVASFCALTKAATSGLLIKGGD LETL KIKIMAFDKTGTITRGEFMVTEFQAL+K+
Sbjct: 363  LSTPVASFCALTKAATSGLLIKGGDCLETLGKIKIMAFDKTGTITRGEFMVTEFQALNKE 422

Query: 430  NISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRIDGK 489
            NIS DTLLYWVSSIESKSSHPMAAALVDHGRSLSI PKPENVDDFQNFPGEGVHGRIDGK
Sbjct: 423  NISLDTLLYWVSSIESKSSHPMAAALVDHGRSLSINPKPENVDDFQNFPGEGVHGRIDGK 482

Query: 490  DIYIGNRKIATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAMDE 549
            DIYIGNRKIATRANC TVP++ DEA+DGRTVGY+FC T+A G F+LSDSCRTGAKEAM E
Sbjct: 483  DIYIGNRKIATRANCETVPEIKDEAKDGRTVGYVFCGTTAAGIFTLSDSCRTGAKEAMAE 542

Query: 550  LRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPTAMIGD 609
            +RSLGIKT MLTGDSSAAALQAQKELGKALE VHAELLPEDKTRLINDFKREGPTAMIGD
Sbjct: 543  IRSLGIKTTMLTGDSSAAALQAQKELGKALETVHAELLPEDKTRLINDFKREGPTAMIGD 602

Query: 610  GLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIENVI 669
            GLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAI+LARRANRKVIENVI
Sbjct: 603  GLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIRLARRANRKVIENVI 662

Query: 670  LSVVPRTAILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSATH 729
            LSV PRTAILGLAFGGHPLVWAAVL DVGACVLVILNSMLLLRGT+GHKGKKAG FSA+H
Sbjct: 663  LSVAPRTAILGLAFGGHPLVWAAVLADVGACVLVILNSMLLLRGTEGHKGKKAGMFSASH 722

Query: 730  CSSKHKCCHVGSHSDEHGSHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSLASGHHHHHHE 789
            CSSKHKCCHVGSHS+EHG HTHDHGC+N SSHSS+HH HH             HHHHHHE
Sbjct: 723  CSSKHKCCHVGSHSEEHGGHTHDHGCSNESSHSSSHHQHHH------------HHHHHHE 782

Query: 790  HKDCGSLKKTHDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAGNVKPCEHGHC 849
            H+DCGSLKKTH+GC  Q C+S CDSGMKNSSSCKKSKLVDS S  DD AG+VKP EH HC
Sbjct: 783  HEDCGSLKKTHNGCSTQKCASTCDSGMKNSSSCKKSKLVDSCSGADDPAGSVKPFEHEHC 842

Query: 850  VHNNQPDEHDH-------DAHLSCA--NHHVEDKHCSPKNTQESCSSQKCASNSCEKIKC 909
            VHNNQPDEH+H       D H  C   NH  E +HC   N  +    + C  N+      
Sbjct: 843  VHNNQPDEHEHCIHNNQPDEHEHCVHNNHPDEHEHCVHNNPPD--EHEHCVHNNRPD--- 902

Query: 910  TSSLASLDGSVDSVELHESECCIHNSQSAQHDH--------EHDHHAHFSCADHHVEEKH 969
                   +  V +    E E C+HN+ + +H+H        EH+HH HFSC DHHVE++H
Sbjct: 903  -----EHEHCVHNNHADEHEHCVHNNHADEHEHCIHNNQPDEHEHHTHFSCDDHHVEDEH 962

Query: 970  CAPKNTQECCSSQKFASSSCEQI--------------SVELHGSGCCTHNSQSAQHDHDI 1029
            C+ KNT E CS  + AS+SCE+I              S ELH SGCCTHN+QSAQHDH+I
Sbjct: 963  CSLKNTLEFCSFPRCASNSCEKIQCTSSPASLDGSAGSDELHESGCCTHNTQSAQHDHEI 1022

Query: 1030 HTLKCDLDDSHLSSPDHHIGHGCCSEKNAQKVSLSPQTCHSETCNLSPCGETKCVDSTAR 1089
             TLKCDLDDSH SSPDHH G+GCCS+KNAQKVSLS   CHSETCN SPCG+TKCVDST +
Sbjct: 1023 QTLKCDLDDSHSSSPDHHNGNGCCSQKNAQKVSLSHPMCHSETCNSSPCGKTKCVDSTEK 1082

Query: 1090 QHAPEGSLELLQDHKHCHQGSFDTSNFVLKSQEKHRKNCTGPCKSRSISVCTEDECRKRL 1149
            QH PEGSLELLQDH HCHQGSFDTSNFVL+SQE HRK+C+GPCKSR +S CTEDEC +R 
Sbjct: 1083 QHTPEGSLELLQDHNHCHQGSFDTSNFVLESQENHRKSCSGPCKSRPLSRCTEDECTERA 1142

Query: 1150 EMIDDCAEAKGHHKMKQHHCDTHLSLRNEGTHPHCKTSNGDEGGAINTAISIKLD-SDHS 1209
            EMI DCAEA  HHKMKQ+HC +H SL NEG HPHCK S GD  GAIN   +I+L+ +DHS
Sbjct: 1143 EMIVDCAEANEHHKMKQYHCHSHSSLENEGVHPHCKASKGDNDGAINKTTNIELEAADHS 1202

Query: 1210 NPKPASACKALENRESNNSCKSCKRGSSQLKIGKSCAALEK-REYGGCCKSYMKECCRKH 1253
             PK  + CKALENRE+NN+CK+C+RGSSQLKIGK+CA L+K RE  GCCKSYM+ECCRKH
Sbjct: 1203 IPKHGNTCKALENRETNNNCKTCRRGSSQLKIGKTCAGLKKNREIRGCCKSYMRECCRKH 1257

BLAST of Lag0033868 vs. NCBI nr
Match: XP_022921515.1 (cadmium/zinc-transporting ATPase HMA3-like [Cucurbita moschata] >XP_022921517.1 cadmium/zinc-transporting ATPase HMA3-like [Cucurbita moschata])

HSP 1 Score: 1923.7 bits (4982), Expect = 0.0e+00
Identity = 1011/1268 (79.73%), Postives = 1091/1268 (86.04%), Query Frame = 0

Query: 10   AAVAGKSGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHDNL 69
            AA A  + GG LQ+SYFDVLGICC+SEIPLIENILKE+EG+KEI+VIVATRT+IV+HDNL
Sbjct: 2    AAAAAGNNGGALQKSYFDVLGICCSSEIPLIENILKEIEGIKEIKVIVATRTLIVLHDNL 61

Query: 70   LLSQAQIVKALNQARLEANVRAYGD-QKNHRKRWPSPYAVASGLLLLLSFLKYVNPIFRW 129
            L+SQAQIVKALNQARLEANVRAYGD QKNHRK+WPSPYAVASGLLLL+SFLKYVNP+F+W
Sbjct: 62   LVSQAQIVKALNQARLEANVRAYGDQQKNHRKKWPSPYAVASGLLLLVSFLKYVNPVFKW 121

Query: 130  VALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIAEW 189
            +ALAAVAAGIWPI LKSF AVR+LRIDINILALIAVIGTIV+ DYLEAATIVFLFTIAEW
Sbjct: 122  MALAAVAAGIWPIALKSFTAVRHLRIDINILALIAVIGTIVLNDYLEAATIVFLFTIAEW 181

Query: 190  LESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIVVE 249
            LESRA HKANAVMSSLLSIAPQKAVLADTG VVGADEVKLGTLLAVKAGEDIPIDGIVVE
Sbjct: 182  LESRAGHKANAVMSSLLSIAPQKAVLADTGVVVGADEVKLGTLLAVKAGEDIPIDGIVVE 241

Query: 250  GKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQ 309
            GKCEVDEKTLTGESFPV KQK+STVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQ
Sbjct: 242  GKCEVDEKTLTGESFPVAKQKDSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQ 301

Query: 310  NNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCALI 369
            N+KSRTQRFID CAKFYTPAVIIIST IAVIP ALRL NRSHWFHLALVVLVSACPCALI
Sbjct: 302  NSKSRTQRFIDKCAKFYTPAVIIISTGIAVIPFALRLHNRSHWFHLALVVLVSACPCALI 361

Query: 370  LSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALDKD 429
            LSTPVASFCALTKAATSGLLIKGGD LETL KIKIMAFDKTGTITRGEFMVTEFQALDK+
Sbjct: 362  LSTPVASFCALTKAATSGLLIKGGDCLETLGKIKIMAFDKTGTITRGEFMVTEFQALDKE 421

Query: 430  NISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRIDGK 489
            NIS DTLLYWVSSIESKSSHPMAAALVDHGRSLSI PKPENVDDFQNFPGEGVHGRIDGK
Sbjct: 422  NISLDTLLYWVSSIESKSSHPMAAALVDHGRSLSINPKPENVDDFQNFPGEGVHGRIDGK 481

Query: 490  DIYIGNRKIATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAMDE 549
            DIYIGNRKIATRANC TVP++ DEA+DGRTVGY+FC T+A G F+LSDSCRTGAKEAM E
Sbjct: 482  DIYIGNRKIATRANCETVPEIKDEAKDGRTVGYVFCGTTAAGIFTLSDSCRTGAKEAMAE 541

Query: 550  LRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPTAMIGD 609
            +RSLGIKT MLTGDSSAAALQAQKELGK LE VHAELLPEDKTRLINDFKREGPTAMIGD
Sbjct: 542  IRSLGIKTTMLTGDSSAAALQAQKELGKDLETVHAELLPEDKTRLINDFKREGPTAMIGD 601

Query: 610  GLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIENVI 669
            GLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAI+LARRANRKVIENVI
Sbjct: 602  GLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIRLARRANRKVIENVI 661

Query: 670  LSVVPRTAILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSATH 729
            LSV PRTAILGLAFGGHPLVWAAVL DVGACVLVILNSMLLLRGT+GHKGKKAG FSA+H
Sbjct: 662  LSVAPRTAILGLAFGGHPLVWAAVLADVGACVLVILNSMLLLRGTEGHKGKKAGMFSASH 721

Query: 730  CSSKHKCCHVGSHSDEHGSHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSLASGHHHHHHE 789
            CSSKHKCCHVGSHS+EHG HTHDHGC+N SSHSS+HH HH             HHHHHHE
Sbjct: 722  CSSKHKCCHVGSHSEEHGGHTHDHGCSNESSHSSSHHQHHH------------HHHHHHE 781

Query: 790  HKDCGSLKKTHDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAGNVKPCEHGHC 849
            H+DCGSL+KTH+GC  Q C+S CDSGMKN SSCKKSKLVDS S+ DD AG+VKP EH HC
Sbjct: 782  HEDCGSLRKTHNGCSTQKCASTCDSGMKNLSSCKKSKLVDSCSRADDPAGSVKPFEHEHC 841

Query: 850  VHNNQPDEHDHDAHLSCANHHVEDKHCSPKNTQESCSSQKCASNSCEKIKCTSSLASLDG 909
            VHNNQPDEH+H  H    N   E +HC   N  +    + C  N+             + 
Sbjct: 842  VHNNQPDEHEHCVH---NNQPDEHEHCVHNNQPD--EHEHCVHNNHPD--------EHEH 901

Query: 910  SVDSVELHESECCIHNSQSAQHDH--------EHDHHAHFSCADHHVEEKHCAPKNTQEC 969
             V +    E E C+HN+   +H+H        EH+HH HFSC DHHVE++HC+ KNT E 
Sbjct: 902  CVHNNRPDEHEHCVHNNHPDEHEHCVHDNQPDEHEHHTHFSCDDHHVEDEHCSLKNTLEF 961

Query: 970  CSSQKFASSSCEQI--------------SVELHGSGCCTHNSQSAQHDHDIHTLKCDLDD 1029
            CS  + AS+SCE+I              S ELH SGCCTHN+QSAQHDH+I TLKCDLDD
Sbjct: 962  CSFPRCASNSCEKIQCTSSPASLDGSAGSDELHESGCCTHNTQSAQHDHEIQTLKCDLDD 1021

Query: 1030 SHLSSPDHHIGHGCCSEKNAQKVSLSPQTCHSETCNLSPCGETKCVDSTARQHAPEGSLE 1089
            SH SSPDHH G+GCCS+KN+QKVSLS   CHSETCN SPCG+TKCVDST +QH PE SLE
Sbjct: 1022 SHSSSPDHHNGNGCCSQKNSQKVSLSHPMCHSETCNSSPCGKTKCVDSTEKQHTPEASLE 1081

Query: 1090 LLQDHKHCHQGSFDTSNFVLKSQEKHRKNCTGPCKSRSISVCTEDECRKRLEMIDDCAEA 1149
            LLQDH HCHQGSFDTSNFVL+SQE H K+C+GPCKSR +S CTEDEC +R EMI DC EA
Sbjct: 1082 LLQDHNHCHQGSFDTSNFVLESQENHGKSCSGPCKSRPLSRCTEDECTERAEMIVDCDEA 1141

Query: 1150 KGHHKMKQHHCDTHLSLRNEGTHPHCKTSNGDEGGAINTAISIKLD-SDHSNPKPASACK 1209
              HHKMKQHHC THLSL NEG HPHCK S GD  GAIN   +IKL+ +DHSNPK  + CK
Sbjct: 1142 NEHHKMKQHHCHTHLSLENEGVHPHCKASKGDNDGAINKTTNIKLEAADHSNPKHGNTCK 1201

Query: 1210 ALENRESNNSCKSCKRGSSQLKIGKSCAALEK-REYGGCCKSYMKECCRKHGDIRTAVRG 1253
            ALENRE+NN+CK+C+RGSSQLKIGK+CA L+K RE  GCCKSYM+ECCRKHGDIR AVRG
Sbjct: 1202 ALENRETNNNCKTCRRGSSQLKIGKTCAGLKKNREIRGCCKSYMRECCRKHGDIRMAVRG 1244

BLAST of Lag0033868 vs. NCBI nr
Match: XP_022987177.1 (cadmium/zinc-transporting ATPase HMA3-like isoform X1 [Cucurbita maxima] >XP_022987178.1 cadmium/zinc-transporting ATPase HMA3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1922.1 bits (4978), Expect = 0.0e+00
Identity = 1008/1270 (79.37%), Postives = 1087/1270 (85.59%), Query Frame = 0

Query: 8    AEAAVAGKSGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHD 67
            A AA A  + GG LQ+SYFDVLGICC+SEIPLIENILKE+EG+KEI+VIVATRT+IV+H 
Sbjct: 4    AAAAEAPGNNGGALQKSYFDVLGICCSSEIPLIENILKEIEGIKEIKVIVATRTLIVLHH 63

Query: 68   NLLLSQAQIVKALNQARLEANVRAYGD-QKNHRKRWPSPYAVASGLLLLLSFLKYVNPIF 127
            NLL+SQAQIVKALNQARLEANVRAYGD QKNHRK+WPSPYAVASGLLLLLSFLKYVNP+F
Sbjct: 64   NLLVSQAQIVKALNQARLEANVRAYGDQQKNHRKKWPSPYAVASGLLLLLSFLKYVNPVF 123

Query: 128  RWVALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIA 187
            +WVALAAVAAGIWPI LKSF AVR+LRIDINILALIAVIGTIV+ DYLEAATIVFLFTIA
Sbjct: 124  KWVALAAVAAGIWPIALKSFTAVRHLRIDINILALIAVIGTIVLNDYLEAATIVFLFTIA 183

Query: 188  EWLESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIV 247
            EWLESRA HKANAVMSSLLSIAPQKAVLADTGEVVGADEVK+GTLLAVKAGEDIPIDGIV
Sbjct: 184  EWLESRAGHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKMGTLLAVKAGEDIPIDGIV 243

Query: 248  VEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE 307
            VEGKCEVDEKTLTGESFPV KQK+STVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE
Sbjct: 244  VEGKCEVDEKTLTGESFPVAKQKDSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE 303

Query: 308  AQNNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCA 367
            AQN+KSRTQRFID CAKFYTPAVIIIST IAVIP ALRL NRSHWFHLALVVLVSACPCA
Sbjct: 304  AQNSKSRTQRFIDKCAKFYTPAVIIISTGIAVIPFALRLHNRSHWFHLALVVLVSACPCA 363

Query: 368  LILSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALD 427
            LILSTPVASFCALTKAATSGLLIKGGD LETL KIKIMAFDKTGTITRGEFMVTEFQALD
Sbjct: 364  LILSTPVASFCALTKAATSGLLIKGGDCLETLGKIKIMAFDKTGTITRGEFMVTEFQALD 423

Query: 428  KDNISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRID 487
            K+NIS DTLLYWVSSIESKSSHPMAAALVDHGRSLSI PKPENVDDFQNFPGEGVHGRID
Sbjct: 424  KENISLDTLLYWVSSIESKSSHPMAAALVDHGRSLSINPKPENVDDFQNFPGEGVHGRID 483

Query: 488  GKDIYIGNRKIATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAM 547
            GKDIYIGNRKIATRANC TVP++ DEA+DGRT+GY+FC T+A G F+LSDSCRTGAKEAM
Sbjct: 484  GKDIYIGNRKIATRANCATVPEIKDEAKDGRTLGYVFCGTTAAGIFTLSDSCRTGAKEAM 543

Query: 548  DELRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPTAMI 607
             E+RSLGIKT MLTGDSSAAALQAQKELGKALE VHAELLPEDKTRLINDFKREGPTAMI
Sbjct: 544  AEIRSLGIKTTMLTGDSSAAALQAQKELGKALETVHAELLPEDKTRLINDFKREGPTAMI 603

Query: 608  GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIEN 667
            GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAI+LARRANRKVIEN
Sbjct: 604  GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIRLARRANRKVIEN 663

Query: 668  VILSVVPRTAILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSA 727
            VILSV PRTAILGLAFGGHPLVWAAVL DVGACVLVILNSMLLLRGT+GHKGKKAG FSA
Sbjct: 664  VILSVAPRTAILGLAFGGHPLVWAAVLADVGACVLVILNSMLLLRGTEGHKGKKAGMFSA 723

Query: 728  THCSSKHKCCHVGSHSDEHGSHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSLASGHHHHH 787
            +HCSSKHKCCHVGSHS+EHG HTHDHGC+N SSHSS+HH HH             HHHHH
Sbjct: 724  SHCSSKHKCCHVGSHSEEHGGHTHDHGCSNESSHSSSHHQHHH------------HHHHH 783

Query: 788  HEHKDCGSLKKTHDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAGNVKPCEHG 847
            HEH+DCGSLKKTH+GC  Q C+S CDSGMK SSSCKKSKLVDS S+ DD AG+VKP EH 
Sbjct: 784  HEHEDCGSLKKTHNGCSTQKCASTCDSGMKKSSSCKKSKLVDSCSRADDPAGSVKPFEHE 843

Query: 848  HCVHNNQPDEHDHDAHLSCANHHVEDKHCSPKNTQESCSSQKCASNSCEKIKCTSSLASL 907
            HCVHNNQP+EH+H  H +  + H +  H +  +  E C                      
Sbjct: 844  HCVHNNQPNEHEHCVHNNHPDEHEQCVHNNHPDEHEHC---------------------- 903

Query: 908  DGSVDSVELHESECCIHNSQSAQHDH--------EHDHHAHFSCADHHVEEKHCAPKNTQ 967
               V + +  E E C+HN+   +H H        EH+HH HFSC DHHVE++HC+ KNT 
Sbjct: 904  ---VHNNQPDEHEHCVHNNHPDEHGHCIHNNQPDEHEHHTHFSCDDHHVEDEHCSLKNTL 963

Query: 968  ECCSSQKFASSSCEQI--------------SVELHGSGCCTHNSQSAQHDHDIHTLKCDL 1027
            E CS  + AS+SCE+I              S ELH  GCCTHN+QSAQHDH+I TLKCDL
Sbjct: 964  EFCSFPRCASNSCEKIQCTSSPANLDGSAGSDELHERGCCTHNTQSAQHDHEIQTLKCDL 1023

Query: 1028 DDSHLSSPDHHIGHGCCSEKNAQKVSLSPQTCHSETCNLSPCGETKCVDSTARQHAPEGS 1087
            DDSH SSPDHH G+GCCS+KNAQKVSLS   CHSETCN SPCG+TKCVDST +QH P+GS
Sbjct: 1024 DDSHSSSPDHHNGNGCCSQKNAQKVSLSHSMCHSETCNSSPCGKTKCVDSTEKQHTPKGS 1083

Query: 1088 LELLQDHKHCHQGSFDTSNFVLKSQEKHRKNCTGPCKSRSISVCTEDECRKRLEMIDDCA 1147
            LELLQDH HCHQGS DTSNFV +SQE HRKNC+GPCKSR IS CTEDEC +R EMI DCA
Sbjct: 1084 LELLQDHNHCHQGSCDTSNFVSESQENHRKNCSGPCKSRPISRCTEDECTERAEMIVDCA 1143

Query: 1148 EAKGHHKMKQHHCDTHLSLRNEGTHPHCKTSNGDEGGAINTAISIKLD-SDHSNPKPASA 1207
            E   HHKMKQHHC THLSL NEG HPHCK S GD  GAIN    I+L+ +DHSNPK  + 
Sbjct: 1144 EGNEHHKMKQHHCHTHLSLENEGVHPHCKASKGDNDGAINKTTKIELEAADHSNPKHGNT 1203

Query: 1208 CKALENRESNNSCKSCKRGSSQLKIGKSCAALEK-REYGGCCKSYMKECCRKHGDIRTAV 1253
            CKALENRE+NN+CK+C+RGSSQLKIG++C  L+K RE  GCCKSYM+ECCRKHGDIR AV
Sbjct: 1204 CKALENRETNNNCKTCRRGSSQLKIGETCVGLKKNREIRGCCKSYMRECCRKHGDIRMAV 1236

BLAST of Lag0033868 vs. NCBI nr
Match: KAG7023376.1 (Cadmium/zinc-transporting ATPase HMA2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1921.7 bits (4977), Expect = 0.0e+00
Identity = 1012/1270 (79.69%), Postives = 1091/1270 (85.91%), Query Frame = 0

Query: 8    AEAAVAGKSGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHD 67
            A AA A  + GG LQ+SYFDVLGICC+SEIPLIENILKE+EG+KEI+VIVATRT+IV+HD
Sbjct: 2    AAAAAAAGNNGGALQKSYFDVLGICCSSEIPLIENILKEIEGIKEIKVIVATRTLIVLHD 61

Query: 68   NLLLSQAQIVKALNQARLEANVRAYGD-QKNHRKRWPSPYAVASGLLLLLSFLKYVNPIF 127
            NLL+SQAQIVKALNQARLEANVRAYGD QKNHRK+WPSPYAVASGLLLL+SFLKYVNP+F
Sbjct: 62   NLLVSQAQIVKALNQARLEANVRAYGDQQKNHRKKWPSPYAVASGLLLLVSFLKYVNPVF 121

Query: 128  RWVALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIA 187
            +WVALAAVAAGIWPI LKSF AVR+LRIDINILALIAVIGTIV+ DYLEAATIVFLFTIA
Sbjct: 122  KWVALAAVAAGIWPIALKSFTAVRHLRIDINILALIAVIGTIVLNDYLEAATIVFLFTIA 181

Query: 188  EWLESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIV 247
            EWLESRA HKANAVMSSLLSIAPQKAVLADTGEVVGADEVK+GTLLAVKAGEDIPIDGIV
Sbjct: 182  EWLESRAGHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKMGTLLAVKAGEDIPIDGIV 241

Query: 248  VEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE 307
            VEGKCEVDEKTLTGESFPV KQK+STVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE
Sbjct: 242  VEGKCEVDEKTLTGESFPVAKQKDSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE 301

Query: 308  AQNNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCA 367
            AQN+KSRTQRFID CAKFYTPAVIIIST IAVIP ALRL NRSHWFHLALVVLVSACPCA
Sbjct: 302  AQNSKSRTQRFIDKCAKFYTPAVIIISTGIAVIPFALRLHNRSHWFHLALVVLVSACPCA 361

Query: 368  LILSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALD 427
            LILSTPVASFCALTKAATSGLLIKGGD LETL KIKIMAFDKTGTITRGEFMVTEFQALD
Sbjct: 362  LILSTPVASFCALTKAATSGLLIKGGDCLETLGKIKIMAFDKTGTITRGEFMVTEFQALD 421

Query: 428  KDNISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRID 487
            K+NIS DTLLYWVSSIESKSSHPMAAALVDHGRSLSI PKPENVDDFQNFPGEGVHGRID
Sbjct: 422  KENISLDTLLYWVSSIESKSSHPMAAALVDHGRSLSINPKPENVDDFQNFPGEGVHGRID 481

Query: 488  GKDIYIGNRKIATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAM 547
            GKDIYIGNRKIATRAN  TVP++ DEA+DGRTVGY+FC T+A G F+LSDSCRTGAKEAM
Sbjct: 482  GKDIYIGNRKIATRANRETVPEIKDEAKDGRTVGYVFCGTTAAGIFTLSDSCRTGAKEAM 541

Query: 548  DELRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPTAMI 607
             E+RSLGIKT MLTGDSSAAALQAQKELGKALE VHAELLPEDKTRLINDFKREGPTAMI
Sbjct: 542  AEIRSLGIKTTMLTGDSSAAALQAQKELGKALETVHAELLPEDKTRLINDFKREGPTAMI 601

Query: 608  GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIEN 667
            GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAI+LARRANRKVIEN
Sbjct: 602  GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIRLARRANRKVIEN 661

Query: 668  VILSVVPRTAILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSA 727
            VILSV PRTAILGLAFGGHPLVWAAVL DVGACVLVILNSMLLLRGT+GHKGKKAG FSA
Sbjct: 662  VILSVAPRTAILGLAFGGHPLVWAAVLADVGACVLVILNSMLLLRGTEGHKGKKAGMFSA 721

Query: 728  THCSSKHKCCHVGSHSDEHGSHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSLASGHHHHH 787
            +HCSSKHKCCHVGSHS+EHG HTHDHGC+N SSHSS+HH HH             HHHHH
Sbjct: 722  SHCSSKHKCCHVGSHSEEHGGHTHDHGCSNESSHSSSHHQHHH------------HHHHH 781

Query: 788  HEHKDCGSLKKTHDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAGNVKPCEHG 847
            HEH+DCGSLKKTH+GC  Q C+S CDSGMKNSSSCKKSKLVDS S  DD  G+VKP EH 
Sbjct: 782  HEHEDCGSLKKTHNGCSTQKCASTCDSGMKNSSSCKKSKLVDSCSGADDPEGSVKPDEHE 841

Query: 848  HCVHNNQPDEHDHDAHLSCANHHVEDKHCSPKNTQESCSSQKCASNSCEKIKCTSSLASL 907
            HCVHNNQPDEH+H  H    N   E +HC   N  +    + C  N+             
Sbjct: 842  HCVHNNQPDEHEHCIH---NNQPDEHEHCVHNNHPD--EHEHCVHNN------------- 901

Query: 908  DGSVDSVELHESECCIHNSQSAQHDH--------EHDHHAHFSCADHHVEEKHCAPKNTQ 967
                      E E C+HN+ + +H+H        EH+HH HFSC DHHVE++HC+ KNT 
Sbjct: 902  -------RPDEHEHCVHNNHADEHEHCIHNNQPDEHEHHTHFSCDDHHVEDEHCSLKNTL 961

Query: 968  ECCSSQKFASSSCEQI--------------SVELHGSGCCTHNSQSAQHDHDIHTLKCDL 1027
            E CS  + AS+SCE+I              S ELH SGCCTHN+QSAQHDH+I TLKCDL
Sbjct: 962  EFCSFPRCASTSCEKIQCTSSPASLDGSAGSDELHESGCCTHNTQSAQHDHEIQTLKCDL 1021

Query: 1028 DDSHLSSPDHHIGHGCCSEKNAQKVSLSPQTCHSETCNLSPCGETKCVDSTARQHAPEGS 1087
            DDSH SSPDHH G+GCCS+KNAQKVSLS   CHSETCN SPCG+TKCVDST +QH PEGS
Sbjct: 1022 DDSHSSSPDHHNGNGCCSQKNAQKVSLSHPMCHSETCNSSPCGKTKCVDSTEKQHTPEGS 1081

Query: 1088 LELLQDHKHCHQGSFDTSNFVLKSQEKHRKNCTGPCKSRSISVCTEDECRKRLEMIDDCA 1147
            LELLQDH HCHQGSFDTSNFVL+SQE HRK+C+GPCKSR +S CTEDEC +R EMI DCA
Sbjct: 1082 LELLQDHNHCHQGSFDTSNFVLESQENHRKSCSGPCKSRPLSRCTEDECTERAEMIVDCA 1141

Query: 1148 EAKGHHKMKQHHCDTHLSLRNEGTHPHCKTSNGDEGGAINTAISIKLD-SDHSNPKPASA 1207
            EA  HHKMKQ+HC +H SL NEG HPHCK S GD  GAIN   +I+L+ +DHS PK  + 
Sbjct: 1142 EANEHHKMKQYHCHSHSSLENEGVHPHCKASKGDNDGAINKTTNIELEAADHSIPKHGNT 1201

Query: 1208 CKALENRESNNSCKSCKRGSSQLKIGKSCAALEK-REYGGCCKSYMKECCRKHGDIRTAV 1253
            CKALENRE+NN+CK+C+RGSSQLKIGK+CA L+K RE  GCCKSYM+ECCRKHGDIR AV
Sbjct: 1202 CKALENRETNNNCKTCRRGSSQLKIGKTCAGLKKNREIRGCCKSYMRECCRKHGDIRMAV 1234

BLAST of Lag0033868 vs. NCBI nr
Match: XP_022987180.1 (cadmium/zinc-transporting ATPase HMA3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1917.9 bits (4967), Expect = 0.0e+00
Identity = 1008/1270 (79.37%), Postives = 1081/1270 (85.12%), Query Frame = 0

Query: 8    AEAAVAGKSGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHD 67
            A AA A  + GG LQ+SYFDVLGICC+SEIPLIENILKE+EG+KEI+VIVATRT+IV+H 
Sbjct: 4    AAAAEAPGNNGGALQKSYFDVLGICCSSEIPLIENILKEIEGIKEIKVIVATRTLIVLHH 63

Query: 68   NLLLSQAQIVKALNQARLEANVRAYGD-QKNHRKRWPSPYAVASGLLLLLSFLKYVNPIF 127
            NLL+SQAQIVKALNQARLEANVRAYGD QKNHRK+WPSPYAVASGLLLLLSFLKYVNP+F
Sbjct: 64   NLLVSQAQIVKALNQARLEANVRAYGDQQKNHRKKWPSPYAVASGLLLLLSFLKYVNPVF 123

Query: 128  RWVALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIA 187
            +WVALAAVAAGIWPI LKSF AVR+LRIDINILALIAVIGTIV+ DYLEAATIVFLFTIA
Sbjct: 124  KWVALAAVAAGIWPIALKSFTAVRHLRIDINILALIAVIGTIVLNDYLEAATIVFLFTIA 183

Query: 188  EWLESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIV 247
            EWLESRA HKANAVMSSLLSIAPQKAVLADTGEVVGADEVK+GTLLAVKAGEDIPIDGIV
Sbjct: 184  EWLESRAGHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKMGTLLAVKAGEDIPIDGIV 243

Query: 248  VEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE 307
            VEGKCEVDEKTLTGESFPV KQK+STVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE
Sbjct: 244  VEGKCEVDEKTLTGESFPVAKQKDSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE 303

Query: 308  AQNNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCA 367
            AQN+KSRTQRFID CAKFYTPAVIIIST IAVIP ALRL NRSHWFHLALVVLVSACPCA
Sbjct: 304  AQNSKSRTQRFIDKCAKFYTPAVIIISTGIAVIPFALRLHNRSHWFHLALVVLVSACPCA 363

Query: 368  LILSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALD 427
            LILSTPVASFCALTKAATSGLLIKGGD LETL KIKIMAFDKTGTITRGEFMVTEFQALD
Sbjct: 364  LILSTPVASFCALTKAATSGLLIKGGDCLETLGKIKIMAFDKTGTITRGEFMVTEFQALD 423

Query: 428  KDNISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRID 487
            K+NIS DTLLYWVSSIESKSSHPMAAALVDHGRSLSI PKPENVDDFQNFPGEGVHGRID
Sbjct: 424  KENISLDTLLYWVSSIESKSSHPMAAALVDHGRSLSINPKPENVDDFQNFPGEGVHGRID 483

Query: 488  GKDIYIGNRKIATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAM 547
            GKDIYIGNRKIATRANC TVP++ DEA+DGRT+GY+FC T+A G F+LSDSCRTGAKEAM
Sbjct: 484  GKDIYIGNRKIATRANCATVPEIKDEAKDGRTLGYVFCGTTAAGIFTLSDSCRTGAKEAM 543

Query: 548  DELRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPTAMI 607
             E+RSLGIKT MLTGDSSAAALQAQKELGKALE VHAELLPEDKTRLINDFKREGPTAMI
Sbjct: 544  AEIRSLGIKTTMLTGDSSAAALQAQKELGKALETVHAELLPEDKTRLINDFKREGPTAMI 603

Query: 608  GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIEN 667
            GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAI+LARRANRKVIEN
Sbjct: 604  GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIRLARRANRKVIEN 663

Query: 668  VILSVVPRTAILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSA 727
            VILSV PRTAILGLAFGGHPLVWAAVL DVGACVLVILNSMLLLRGT+GHKGKKAG FSA
Sbjct: 664  VILSVAPRTAILGLAFGGHPLVWAAVLADVGACVLVILNSMLLLRGTEGHKGKKAGMFSA 723

Query: 728  THCSSKHKCCHVGSHSDEHGSHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSLASGHHHHH 787
            +HCSSKHKCCHVGSHS+EHG HTHDHGC+N SSHSS+HH HH             HHHHH
Sbjct: 724  SHCSSKHKCCHVGSHSEEHGGHTHDHGCSNESSHSSSHHQHHH------------HHHHH 783

Query: 788  HEHKDCGSLKKTHDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAGNVKPCEHG 847
            HEH+DCGSLKKTH+GC  Q C+S CDSGMK SSSCKKSKLVDS S+ DD AG+VKP EH 
Sbjct: 784  HEHEDCGSLKKTHNGCSTQKCASTCDSGMKKSSSCKKSKLVDSCSRADDPAGSVKPFEHE 843

Query: 848  HCVHNNQPDEHDHDAHLSCANHHVEDKHCSPKNTQESCSSQKCASNSCEKIKCTSSLASL 907
            HCVHNN PDEH+   H    NH  E +HC   N  +                        
Sbjct: 844  HCVHNNHPDEHEQCVH---NNHPDEHEHCVHNNQPD------------------------ 903

Query: 908  DGSVDSVELHESECCIHNSQSAQHDH--------EHDHHAHFSCADHHVEEKHCAPKNTQ 967
                      E E C+HN+   +H H        EH+HH HFSC DHHVE++HC+ KNT 
Sbjct: 904  ----------EHEHCVHNNHPDEHGHCIHNNQPDEHEHHTHFSCDDHHVEDEHCSLKNTL 963

Query: 968  ECCSSQKFASSSCEQI--------------SVELHGSGCCTHNSQSAQHDHDIHTLKCDL 1027
            E CS  + AS+SCE+I              S ELH  GCCTHN+QSAQHDH+I TLKCDL
Sbjct: 964  EFCSFPRCASNSCEKIQCTSSPANLDGSAGSDELHERGCCTHNTQSAQHDHEIQTLKCDL 1023

Query: 1028 DDSHLSSPDHHIGHGCCSEKNAQKVSLSPQTCHSETCNLSPCGETKCVDSTARQHAPEGS 1087
            DDSH SSPDHH G+GCCS+KNAQKVSLS   CHSETCN SPCG+TKCVDST +QH P+GS
Sbjct: 1024 DDSHSSSPDHHNGNGCCSQKNAQKVSLSHSMCHSETCNSSPCGKTKCVDSTEKQHTPKGS 1083

Query: 1088 LELLQDHKHCHQGSFDTSNFVLKSQEKHRKNCTGPCKSRSISVCTEDECRKRLEMIDDCA 1147
            LELLQDH HCHQGS DTSNFV +SQE HRKNC+GPCKSR IS CTEDEC +R EMI DCA
Sbjct: 1084 LELLQDHNHCHQGSCDTSNFVSESQENHRKNCSGPCKSRPISRCTEDECTERAEMIVDCA 1143

Query: 1148 EAKGHHKMKQHHCDTHLSLRNEGTHPHCKTSNGDEGGAINTAISIKLD-SDHSNPKPASA 1207
            E   HHKMKQHHC THLSL NEG HPHCK S GD  GAIN    I+L+ +DHSNPK  + 
Sbjct: 1144 EGNEHHKMKQHHCHTHLSLENEGVHPHCKASKGDNDGAINKTTKIELEAADHSNPKHGNT 1203

Query: 1208 CKALENRESNNSCKSCKRGSSQLKIGKSCAALEK-REYGGCCKSYMKECCRKHGDIRTAV 1253
            CKALENRE+NN+CK+C+RGSSQLKIG++C  L+K RE  GCCKSYM+ECCRKHGDIR AV
Sbjct: 1204 CKALENRETNNNCKTCRRGSSQLKIGETCVGLKKNREIRGCCKSYMRECCRKHGDIRMAV 1224

BLAST of Lag0033868 vs. ExPASy Swiss-Prot
Match: Q9SZW4 (Cadmium/zinc-transporting ATPase HMA2 OS=Arabidopsis thaliana OX=3702 GN=HMA2 PE=2 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 5.9e-268
Identity = 548/979 (55.98%), Postives = 681/979 (69.56%), Query Frame = 0

Query: 21  LQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHDNLLLSQAQIVKAL 80
           + +SYFDVLGICC SE+PLIENIL  M+GVKE  VIV +RTVIVVHD L+LSQ QIVKAL
Sbjct: 6   MTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQIVKAL 65

Query: 81  NQARLEANVRAYGDQKNHRKRWPSPYAVASGLLLLLSFLKYVNPIFRWVALAAVAAGIWP 140
           NQA+LEANVR  G + N + +WPSP+AV SG+LLLLSF KY+   FRW+A+AAV AGI+P
Sbjct: 66  NQAQLEANVRVTG-ETNFKNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVVAGIYP 125

Query: 141 IVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIAEWLESRASHKANAV 200
           I+ K+ A++   RIDINIL ++ V  TI M+DY EAA +VFLFTIAEWL+SRAS+KA+AV
Sbjct: 126 ILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASYKASAV 185

Query: 201 MSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIVVEGKCEVDEKTLTG 260
           M SL+S+APQKAV+A+TGE V  DE+K  T++AVKAGE IPIDG+VV+G CEVDEKTLTG
Sbjct: 186 MQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDEKTLTG 245

Query: 261 ESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQRFIDI 320
           E+FPVPK K+STVWAGTINLNGY+TV TTALAEDCVVAKMAKLVEEAQN+K+ TQRFID 
Sbjct: 246 EAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQRFIDK 305

Query: 321 CAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCALILSTPVASFCALT 380
           C+K+YTPA+I+IS C   IP AL++ N  HW HLALVVLVSACPC LILSTPVA+FCALT
Sbjct: 306 CSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVATFCALT 365

Query: 381 KAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALDKDNISFDTLLYWVS 440
           KAATSGLLIKG D+LETLAKIKI+AFDKTGTITRGEF+V +FQ+L +D IS  +LLYWVS
Sbjct: 366 KAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSLSED-ISLQSLLYWVS 425

Query: 441 SIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRIDGKDIYIGNRKIATR 500
           S ESKSSHPMAAA+VD+ RS+S+ PKPE V+D+QNFPGEG++G+IDGK++YIGN++IA+R
Sbjct: 426 STESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRIASR 485

Query: 501 ANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAMDELRSLGIKTAMLT 560
           A C +VPD++ + + G+T+GY++   +  G F+LSD+CR+G  +AM EL+SLGIK AMLT
Sbjct: 486 AGCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLGIKIAMLT 545

Query: 561 GDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKR-EGPTAMIGDGLNDAPALATA 620
           GD+ AAA+ AQ++LG A++IV AELLPEDK+ +I   KR EGPTAM+GDGLNDAPALATA
Sbjct: 546 GDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMVGDGLNDAPALATA 605

Query: 621 DIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIENVILSVVPRTAILG 680
           DIGISMG+SGSALA ETG++ILM+NDIR+IP+AIKLA+RA RKV+ENV++S+  + AIL 
Sbjct: 606 DIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKGAILA 665

Query: 681 LAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHK---------------------G 740
           LAF GHPL+WAAVL DVG C+LVILNSMLLL  +D HK                     G
Sbjct: 666 LAFAGHPLIWAAVLADVGTCLLVILNSMLLL--SDKHKTGNKCYRESSSSSVLIAEKLEG 725

Query: 741 KKAG--------KFSATHCSSKHKCCHVGSHSDE------HGSHTHDH-GC--------- 800
             AG        K S  HC  K  CC  G+ + E        S  H H GC         
Sbjct: 726 DAAGDMEAGLLPKISDKHC--KPGCC--GTKTQEKAMKPAKASSDHSHSGCCETKQKDNV 785

Query: 801 ----NNGSSHSSNHHHHHDHGCNNDSSLASGHHHHHHEHKDC-------------GSLKK 860
                +  +   +  H HD GC  D S    H H     + C             G  ++
Sbjct: 786 TVVKKSCCAEPVDLGHGHDSGCCGDKS-QQPHQHEVQVQQSCHNKPSGLDSGCCGGKSQQ 845

Query: 861 THDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAGNVKPCEHGHCVHNNQPDEH 920
            H   L Q+C  K  SG+   +  K        +   D+   +K   +G C         
Sbjct: 846 PHQHELQQSCHDK-PSGLDIGTGPKHEGSSTLVNLEGDAKEELKVLVNGFC--------- 905

Query: 921 DHDAHLSCANHHVE-DKHCSPKNTQESCSSQKCASNSCEKIKCTSSLASLDGSVDSVELH 935
              A L+  +  V+ D HC     + +CSS++         +C                H
Sbjct: 906 SSPADLAITSLKVKSDSHC-----KSNCSSRE---------RCH---------------H 936

BLAST of Lag0033868 vs. ExPASy Swiss-Prot
Match: O64474 (Putative cadmium/zinc-transporting ATPase HMA4 OS=Arabidopsis thaliana OX=3702 GN=HMA4 PE=1 SV=2)

HSP 1 Score: 924.1 bits (2387), Expect = 1.7e-267
Identity = 565/1173 (48.17%), Postives = 765/1173 (65.22%), Query Frame = 0

Query: 21   LQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHDNLLLSQAQIVKAL 80
            LQ+SYFDVLGICC SE+P+IENILK ++GVKE  VIV +RTVIVVHD+LL+S  QI KAL
Sbjct: 16   LQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSLLISPFQIAKAL 75

Query: 81   NQARLEANVRAYGDQKNHRKRWPSPYAVASGLLLLLSFLKYVNPIFRWVALAAVAAGIWP 140
            N+ARLEANVR  G + + + +WPSP+AV SGLLLLLSFLK+V    RW+A+AAVAAGI+P
Sbjct: 76   NEARLEANVRVNG-ETSFKNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLAVAAVAAGIYP 135

Query: 141  IVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIAEWLESRASHKANAV 200
            I+ K+FA+++  RIDINIL +I VI T+ M+D++EAA +VFLFTI++WLE+RAS+KA +V
Sbjct: 136  ILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLETRASYKATSV 195

Query: 201  MSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIVVEGKCEVDEKTLTG 260
            M SL+S+APQKA++A+TGE V  DEVK+ T++AVKAGE IPIDGIVV+G CEVDEKTLTG
Sbjct: 196  MQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGNCEVDEKTLTG 255

Query: 261  ESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQRFIDI 320
            E+FPVPKQ++STVWAGTINLNGY+ VKTT+LA DCVVAKMAKLVEEAQ++K+++QR ID 
Sbjct: 256  EAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSSKTKSQRLIDK 315

Query: 321  CAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCALILSTPVASFCALT 380
            C+++YTPA+I++S C+A++PV +++ N  HWFHLALVVLVS CPC LILSTPVA+FCALT
Sbjct: 316  CSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILSTPVATFCALT 375

Query: 381  KAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALDKDNISFDTLLYWVS 440
            KAATSGLLIK  D+L+TL+KIKI+AFDKTGTITRGEF+V +F++L +D I+  +LLYWVS
Sbjct: 376  KAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSLSRD-INLRSLLYWVS 435

Query: 441  SIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRIDGKDIYIGNRKIATR 500
            S+ESKSSHPMAA +VD+ +S+S+ P+PE V+D+QNFPGEG++G+IDG DI+IGN+KIA+R
Sbjct: 436  SVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFIGNKKIASR 495

Query: 501  ANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAMDELRSLGIKTAMLT 560
            A C+TVP++  + + G+TVGY++      G F+LSD+CR+G  +AM EL+SLGIKTAMLT
Sbjct: 496  AGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKSLGIKTAMLT 555

Query: 561  GDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPTAMIGDGLNDAPALATAD 620
            GD+ AAA+ AQ++LG  L++VH +LLPEDK+R+I +FK+EGPTAM+GDG+NDAPALATAD
Sbjct: 556  GDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKEGPTAMVGDGVNDAPALATAD 615

Query: 621  IGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIENVILSVVPRTAILGL 680
            IGISMGISGSALA +TG++ILM+NDIR+IP+A+KLARRA RKV+ENV LS++ +  IL L
Sbjct: 616  IGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLSIILKAGILAL 675

Query: 681  AFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSATHCSSKHKCCHVGS 740
            AF GHPL+WAAVLVDVG C+LVI NSMLLLR       KK  + S +  + +        
Sbjct: 676  AFAGHPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKKCYRASTSKLNGRKLEGDDDY 735

Query: 741  HSD-EHGSHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSLASGHHHHHHEHKDCGSLKK-- 800
              D E G  T        SS   +  +  +      SS  S  H H      CG  K+  
Sbjct: 736  VVDLEAGLLTKSGNGQCKSSCCGDKKNQENVVMMKPSSKTSSDHSH---PGCCGDKKEEK 795

Query: 801  ----THDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAG--------------- 860
                  DGC  +  + K +  M + SSCKKS  V    K+   +G               
Sbjct: 796  VKPLVKDGCCSEK-TRKSEGDMVSLSSCKKSSHVKHDLKMKGGSGCCASKNEKGKEVVAK 855

Query: 861  -----------NVKPCEHGHCVHNNQPDEHDHDAH-LSCANHHVEDKHCSPKNTQESCSS 920
                       +V  C+ GHC    Q ++       +  A  HVE +      T+E+C +
Sbjct: 856  SCCEKPKQQVESVGDCKSGHCEKKKQAEDIVVPVQIIGHALTHVEIE----LQTKETCKT 915

Query: 921  QKCASNSCEKIKCTSSLASLDGS---VDSVELHESECCIHNSQSAQHDHEHDHHAHFS-- 980
              C  +S EK+K T  L S + +      V + +   C   S++     +  H    S  
Sbjct: 916  SCC--DSKEKVKETGLLLSSENTPYLEKGVLIKDEGNCKSGSENMGTVKQSCHEKGCSDE 975

Query: 981  --------CADHHVEEKHCAP-----KNTQECCSSQKFASSSCEQISVELHGSGCCTHNS 1040
                     ++   +++ C+      + T +    +K  S   E+  +++    CC    
Sbjct: 976  KQTGEITLASEEETDDQDCSSGCCVNEGTVKQSFDEKKHSVLVEKEGLDMETGFCCDAKL 1035

Query: 1041 QSAQHDHDIHTLKCDLDDSHLSSPDHHIGHGCCSE--KNAQKVSLSPQTCHSETCNLSPC 1100
                +       +C L+       + H   GCC E  +  +   +S +   S  C+   C
Sbjct: 1036 VCCGNTEGEVKEQCRLE----IKKEEHCKSGCCGEEIQTGEITLVSEEETESTNCSTGCC 1095

Query: 1101 GETKCVDSTARQHAPE---GSLELLQDHKHCHQGSFDTSNFVLKSQEKHRKNCTGPCKSR 1137
             + + V  T  +         LE+ +D +HC + S          + K  + C   C+ R
Sbjct: 1096 VDKEEVTQTCHEKPASLVVSGLEVKKD-EHC-ESSHRAVKVETCCKVKIPEACASKCRDR 1155

BLAST of Lag0033868 vs. ExPASy Swiss-Prot
Match: P0CW78 (Cadmium/zinc-transporting ATPase HMA3 OS=Arabidopsis thaliana OX=3702 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 5.1e-256
Identity = 458/713 (64.24%), Postives = 578/713 (81.07%), Query Frame = 0

Query: 1   MAAGEEKAEAAVAGKSGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATR 60
           MA GEE  +           LQ SYFDV+GICC+SE+ ++ N+L++++GVKE  VIV +R
Sbjct: 1   MAEGEESKKM---------NLQTSYFDVVGICCSSEVSIVGNVLRQVDGVKEFSVIVPSR 60

Query: 61  TVIVVHDNLLLSQAQIVKALNQARLEANVRAYGDQKNHRKRWPSPYAVASGLLLLLSFLK 120
           TVIVVHD  L+S  QIVKALNQARLEA+VR YG + + + +WPSP+A+ SG+LL+LSF K
Sbjct: 61  TVIVVHDTFLISPLQIVKALNQARLEASVRPYG-ETSLKSQWPSPFAIVSGVLLVLSFFK 120

Query: 121 YVNPIFRWVALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIV 180
           Y      W+A+ AV AG++PI+ K+ A+V   R+DIN L LIAVI T+ M+D+ EAATIV
Sbjct: 121 YFYSPLEWLAIVAVVAGVFPILAKAVASVTRFRLDINALTLIAVIATLCMQDFTEAATIV 180

Query: 181 FLFTIAEWLESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDI 240
           FLF++A+WLES A+HKA+ VMSSL+S+AP+KAV+ADTG  V  DEV + T+++VKAGE I
Sbjct: 181 FLFSVADWLESSAAHKASIVMSSLMSLAPRKAVIADTGLEVDVDEVGINTVVSVKAGESI 240

Query: 241 PIDGIVVEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKM 300
           PIDG+VV+G C+VDEKTLTGESFPV KQ+ STV A TINLNGY+ VKTTALA DCVVAKM
Sbjct: 241 PIDGVVVDGSCDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTTALARDCVVAKM 300

Query: 301 AKLVEEAQNNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLV 360
            KLVEEAQ ++++TQRFID C+++YTPAV++ + C AVIPV L++ + SHWFHLALVVLV
Sbjct: 301 TKLVEEAQKSQTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVLV 360

Query: 361 SACPCALILSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVT 420
           S CPC LILSTPVA+FCALTKAATSG LIK GD LETLAKIKI+AFDKTGTIT+ EFMV+
Sbjct: 361 SGCPCGLILSTPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMVS 420

Query: 421 EFQALDKDNISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEG 480
           +F++L   +I+   LL WVSSIE KSSHPMAAAL+D+  S+S+ PKP+ V++FQNFPGEG
Sbjct: 421 DFRSL-SPSINLHKLLNWVSSIECKSSHPMAAALIDYAISVSVEPKPDIVENFQNFPGEG 480

Query: 481 VHGRIDGKDIYIGNRKIATRANCTT--VPDMNDEARDGRTVGYIFCETSAVGTFSLSDSC 540
           V+GRIDG+DIYIGN++IA RA C T  VPD+    + G+T+GYI+      G+F+L D C
Sbjct: 481 VYGRIDGQDIYIGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLLDGC 540

Query: 541 RTGAKEAMDELRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFK 600
           R G  +A+ EL+SLGI+TAMLTGD+  AA+  Q++L  AL+IVH+ELLP+DK R+I+DFK
Sbjct: 541 RYGVAQALKELKSLGIQTAMLTGDNQDAAMSTQEQLENALDIVHSELLPQDKARIIDDFK 600

Query: 601 REGPTAMIGDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARR 660
            +GPT M+GDGLNDAPALA ADIGISMGISGSALA ETGD+ILM+NDIRKIPK ++LA+R
Sbjct: 601 IQGPTMMVGDGLNDAPALAKADIGISMGISGSALATETGDIILMSNDIRKIPKGMRLAKR 660

Query: 661 ANRKVIENVILSVVPRTAILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLR 712
           +++KVIENV+LSV  + AI+ L F G+PLVWAAVL D G C+LVILNSM+LLR
Sbjct: 661 SHKKVIENVVLSVSIKGAIMVLGFVGYPLVWAAVLADAGTCLLVILNSMILLR 702

BLAST of Lag0033868 vs. ExPASy Swiss-Prot
Match: A3BF39 (Cadmium/zinc-transporting ATPase HMA2 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA2 PE=1 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.2e-252
Identity = 533/1102 (48.37%), Postives = 691/1102 (62.70%), Query Frame = 0

Query: 18   GGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHDNLLLSQAQIV 77
            GG  Q+SYFDVLGICC SE+PL+E +L+ +EGV+++ VIV +RTVIVVHD   +SQ+QIV
Sbjct: 5    GGRCQKSYFDVLGICCPSEVPLVEKLLQPLEGVQKVTVIVPSRTVIVVHDVDAISQSQIV 64

Query: 78   KALNQARLEANVRAYGD-QKNHRKRWPSPYAVASGLLLLLSFLKYVNPIFRWVALAAVAA 137
            KALNQARLEA+VRAYG+  +    +WPSPY +  GLLL++S  ++     +W AL A AA
Sbjct: 65   KALNQARLEASVRAYGNGSEKITNKWPSPYVLLCGLLLVVSLFEHFWHPLKWFALVAAAA 124

Query: 138  GIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIAEWLESRASHK 197
            G+ PIVL+S AA+R L +D+NIL LIAV G I +KDY EA  IVFLFT AEWLE+RASHK
Sbjct: 125  GLPPIVLRSIAAIRRLTLDVNILMLIAVAGAIALKDYSEAGFIVFLFTTAEWLETRASHK 184

Query: 198  ANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIVVEGKCEVDEK 257
            A A MS+L+S+APQKA+LA+TGEVV A +VK+ T++AVKAGE IPIDG+VV+G+ EVDE 
Sbjct: 185  ATAGMSALMSMAPQKAILAETGEVVAARDVKVNTVIAVKAGEVIPIDGVVVDGRSEVDES 244

Query: 258  TLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQR 317
            TLTGESFPV KQ +S VWAGT+N++GY+ V+TTA+A++  VAKMA+LVEEAQN++S TQR
Sbjct: 245  TLTGESFPVSKQPDSQVWAGTLNIDGYIAVRTTAMADNSAVAKMARLVEEAQNSRSSTQR 304

Query: 318  FIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCALILSTPVASF 377
             ID CAK+YTPAV++++  +A IP   +  N  HWF LALV+LVSACPCAL+LSTP+A+F
Sbjct: 305  LIDTCAKYYTPAVVVMAGSVAAIPAIAKAHNLKHWFQLALVLLVSACPCALVLSTPIATF 364

Query: 378  CALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALDKDNISFDTLL 437
            CAL +AA +GLLIKGGD LE+LA IK+ AFDKTGTITRGEF V EFQ +  + +S   LL
Sbjct: 365  CALLRAARTGLLIKGGDVLESLASIKVAAFDKTGTITRGEFSVEEFQPVG-ERVSLQQLL 424

Query: 438  YWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRIDGKDIYIGNRK 497
            YWVSS+ES+SSHPMA+ LVD+ +S S+ PK ENV +FQ +PGEG++G IDG  IYIGN++
Sbjct: 425  YWVSSVESRSSHPMASVLVDYAQSKSVEPKSENVSEFQIYPGEGIYGEIDGAGIYIGNKR 484

Query: 498  IATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAMDELRSLGIKT 557
            I +RA+C TVPDM D    G T+GY+ C    +G F+LSD+CRTG+ EA+ ELRSLGIK+
Sbjct: 485  ILSRASCETVPDMKD--MKGVTIGYVACNNELIGVFTLSDACRTGSAEAIKELRSLGIKS 544

Query: 558  AMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFK-REGPTAMIGDGLNDAPA 617
             MLTGDSSAAA  AQ +LG  L  VHAELLPEDK R++ + K ++GPT M+GDG+NDAPA
Sbjct: 545  VMLTGDSSAAATYAQNQLGNILAEVHAELLPEDKVRIVGELKEKDGPTLMVGDGMNDAPA 604

Query: 618  LATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIENVILSVVPRT 677
            LA AD+G+SMG+SGSA+A+ET  V LM+NDIR+IPKA++LARR +R +I N+I SV+ + 
Sbjct: 605  LAKADVGVSMGVSGSAVAMETSHVALMSNDIRRIPKAVRLARRTHRTIIVNIIFSVITKL 664

Query: 678  AILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSATHCSSKHKC 737
            AI+GLAF GHPL+WAAVL DVG C+LVI+ SMLLLR  D  K KK    +A+H  S  KC
Sbjct: 665  AIVGLAFAGHPLIWAAVLADVGTCLLVIMYSMLLLREKDSRKAKKC---AASHHGSPKKC 724

Query: 738  C---HVGSHSDEHGSHTHDHGCNNG----------SSHSSN--HHHHHDHGCNNDSSLAS 797
            C   H GSH+ +  +H   H C++G          SS + N  H HHH+H          
Sbjct: 725  CSSSHHGSHAKK--NHGVSHHCSDGPCKSMVSCKESSVAKNACHDHHHEH---------- 784

Query: 798  GHHHHHHEHKDCGSLKKTHDGCL-PQNCSSKCDSGMKNSSSCK--KSKLVDSSSKVDDSA 857
             +HH    HK   +    HD      NC    +  + N  +C    +   D+S+  D   
Sbjct: 785  -NHHEEPAHKHSSNQHGCHDHSHGHSNCKEPSNQLITNKHACHDGHNHCADTSNLHDTKK 844

Query: 858  GNVKPCEHGHCVH--NNQPDEHDHDAHLSCANHHVEDKHCSPKNTQESCSSQKCASNSCE 917
             +    EH  C    N  P  +DH    +C  H  E  HC       S     C  +  E
Sbjct: 845  HDCHGHEHSTCKEELNALPPTNDH----ACHGH--EHSHCEEPVALHSTGEHACHEHEHE 904

Query: 918  KIKCT----SSLASLDGSVDSVELHESECCIHNSQSAQ------HDHEHDHHAHFSCADH 977
             I C     S  A      D  ++HE  CC              HDH+HD+       D 
Sbjct: 905  HIHCDEPIGSHCADKHACHDHEQVHEHHCCDEQQTPHTADLHPCHDHDHDNLEVEEVKDC 964

Query: 978  HVEEKHCAPKNTQECCSSQKFASSSCEQISVELHGSGCCTHNSQSAQHD-HDIHTLKCDL 1037
            H E  H                           H + CC       ++D H +      +
Sbjct: 965  HAEPPH---------------------------HHNHCCHEPHDQVKNDTHPVQEHSISI 1024

Query: 1038 DDSHLSSPDHHIGH--------GCCSEKNAQKVSLSPQTCHSETCNLSPCGETKCVDSTA 1079
            ++    S DHH  H         C      +  +  P  C S  C  +         S  
Sbjct: 1025 EE----SSDHHEHHHNEEHKAEDCGHHPKPKDCAPPPTDCISRNCCSNTSKGKDICSSLH 1050

BLAST of Lag0033868 vs. ExPASy Swiss-Prot
Match: Q8H384 (Cadmium/zinc-transporting ATPase HMA3 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 2.8e-217
Identity = 403/729 (55.28%), Postives = 527/729 (72.29%), Query Frame = 0

Query: 2   AAGEEKAEAAVAGKSGGGGLQR--SYFDVLGICCASEIPLIENILKEMEGVKEIRVIVAT 61
           AA EE       G  GGGG +R  +Y DVLG+CC++E+ L+E +L  ++GV+ + V+VA+
Sbjct: 21  AAAEEPTRCG-GGDGGGGGRKRKKTYLDVLGVCCSAEVALVERLLAPLDGVRVVSVVVAS 80

Query: 62  RTVIVVHDNLLLSQAQIVKALNQARLEANVRAYGDQKNHRKRWPSPYAVASGLLLLLSFL 121
           RTV+V HD     ++ IVKALN+A LEA+VRAYG       RWPSPY VASG+LL  SF 
Sbjct: 81  RTVVVEHDPAAAPESAIVKALNKAGLEASVRAYG-SSGVVSRWPSPYIVASGVLLTASFF 140

Query: 122 KYVNPIFRWVALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATI 181
           +++ P  + +A+AAV AG  P+V + FAA   L +DIN+L LIAV G + + DY EA  I
Sbjct: 141 EWLFPPLQCLAVAAVVAGAPPMVRRGFAAASRLSLDINVLMLIAVAGALCLGDYTEAGAI 200

Query: 182 VFLFTIAEWLESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGED 241
           VFLFT AEWLE+ A  KA+A MSSL+ + P KAV+A TGEVV   +V++G ++AV+AGE 
Sbjct: 201 VFLFTTAEWLETLACTKASAGMSSLMGMLPVKAVIATTGEVVSVRDVRVGDVVAVRAGEI 260

Query: 242 IPIDGIVVEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAK 301
           +P+DG+VV+G+ EVDE++LTGESFPVPKQ +S VWAGT+N +GY+ V+TTALAE+  VAK
Sbjct: 261 VPVDGVVVDGQSEVDERSLTGESFPVPKQPHSEVWAGTMNFDGYIAVRTTALAENSTVAK 320

Query: 302 MAKLVEEAQNNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVL 361
           M +LVE AQN++S+TQR ID CAK+YTPAV++++  +A+IP  L       W+ LALV+L
Sbjct: 321 MERLVEAAQNSRSKTQRLIDSCAKYYTPAVVVVAAGVALIPALLGADGLEQWWKLALVML 380

Query: 362 VSACPCALILSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMV 421
           VSACPCAL+LSTPVASFCA+ +AA  G+ IKGGD LE+L +I+ +AFDKTGTITRGEF +
Sbjct: 381 VSACPCALVLSTPVASFCAMLRAARMGIFIKGGDVLESLGEIRAVAFDKTGTITRGEFSI 440

Query: 422 TEFQALDKDNISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGE 481
             F  +    +  D LLYW++SIESKSSHPMAAALV++ +S SI P PENV DF+ +PGE
Sbjct: 441 DSFHLVGDHKVEMDHLLYWIASIESKSSHPMAAALVEYAQSKSIQPNPENVGDFRIYPGE 500

Query: 482 GVHGRIDGKDIYIGNRKIATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCR 541
           G++G I GK IYIGNR+   RA+         E   G ++GY+ C+    G FSLSD CR
Sbjct: 501 GIYGEIHGKHIYIGNRRTLARASSPQSTQEMGEMIKGVSIGYVICDGELAGVFSLSDDCR 560

Query: 542 TGAKEAMDELRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFK- 601
           TGA EA+ EL SLGIK+ MLTGDSSAAA  AQ +LG  +E +H+ELLPEDK RL++  K 
Sbjct: 561 TGAAEAIRELGSLGIKSVMLTGDSSAAATHAQGQLGGVMEELHSELLPEDKVRLVSGLKA 620

Query: 602 REGPTAMIGDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARR 661
           R GPT M+GDG+NDA ALA AD+G+SMGISGSA A+ET    LM++D+ ++P+A++L R 
Sbjct: 621 RFGPTMMVGDGMNDAAALAAADVGVSMGISGSAAAMETSHATLMSSDVLRVPEAVRLGRC 680

Query: 662 ANRKVIENVILSVVPRTAILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKG 721
           A R +  NV  SV  + A+L LA    P++WAAVL DVG C+LV+LNSM LLR       
Sbjct: 681 ARRTIAVNVAGSVAVKAAVLALAAAWRPVLWAAVLADVGTCLLVVLNSMTLLREEWKGGA 740

Query: 722 KKAGKFSAT 728
           K+ G   AT
Sbjct: 741 KEDGACRAT 747

BLAST of Lag0033868 vs. ExPASy TrEMBL
Match: A0A6J1E601 (cadmium/zinc-transporting ATPase HMA3-like OS=Cucurbita moschata OX=3662 GN=LOC111429757 PE=3 SV=1)

HSP 1 Score: 1923.7 bits (4982), Expect = 0.0e+00
Identity = 1011/1268 (79.73%), Postives = 1091/1268 (86.04%), Query Frame = 0

Query: 10   AAVAGKSGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHDNL 69
            AA A  + GG LQ+SYFDVLGICC+SEIPLIENILKE+EG+KEI+VIVATRT+IV+HDNL
Sbjct: 2    AAAAAGNNGGALQKSYFDVLGICCSSEIPLIENILKEIEGIKEIKVIVATRTLIVLHDNL 61

Query: 70   LLSQAQIVKALNQARLEANVRAYGD-QKNHRKRWPSPYAVASGLLLLLSFLKYVNPIFRW 129
            L+SQAQIVKALNQARLEANVRAYGD QKNHRK+WPSPYAVASGLLLL+SFLKYVNP+F+W
Sbjct: 62   LVSQAQIVKALNQARLEANVRAYGDQQKNHRKKWPSPYAVASGLLLLVSFLKYVNPVFKW 121

Query: 130  VALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIAEW 189
            +ALAAVAAGIWPI LKSF AVR+LRIDINILALIAVIGTIV+ DYLEAATIVFLFTIAEW
Sbjct: 122  MALAAVAAGIWPIALKSFTAVRHLRIDINILALIAVIGTIVLNDYLEAATIVFLFTIAEW 181

Query: 190  LESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIVVE 249
            LESRA HKANAVMSSLLSIAPQKAVLADTG VVGADEVKLGTLLAVKAGEDIPIDGIVVE
Sbjct: 182  LESRAGHKANAVMSSLLSIAPQKAVLADTGVVVGADEVKLGTLLAVKAGEDIPIDGIVVE 241

Query: 250  GKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQ 309
            GKCEVDEKTLTGESFPV KQK+STVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQ
Sbjct: 242  GKCEVDEKTLTGESFPVAKQKDSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQ 301

Query: 310  NNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCALI 369
            N+KSRTQRFID CAKFYTPAVIIIST IAVIP ALRL NRSHWFHLALVVLVSACPCALI
Sbjct: 302  NSKSRTQRFIDKCAKFYTPAVIIISTGIAVIPFALRLHNRSHWFHLALVVLVSACPCALI 361

Query: 370  LSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALDKD 429
            LSTPVASFCALTKAATSGLLIKGGD LETL KIKIMAFDKTGTITRGEFMVTEFQALDK+
Sbjct: 362  LSTPVASFCALTKAATSGLLIKGGDCLETLGKIKIMAFDKTGTITRGEFMVTEFQALDKE 421

Query: 430  NISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRIDGK 489
            NIS DTLLYWVSSIESKSSHPMAAALVDHGRSLSI PKPENVDDFQNFPGEGVHGRIDGK
Sbjct: 422  NISLDTLLYWVSSIESKSSHPMAAALVDHGRSLSINPKPENVDDFQNFPGEGVHGRIDGK 481

Query: 490  DIYIGNRKIATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAMDE 549
            DIYIGNRKIATRANC TVP++ DEA+DGRTVGY+FC T+A G F+LSDSCRTGAKEAM E
Sbjct: 482  DIYIGNRKIATRANCETVPEIKDEAKDGRTVGYVFCGTTAAGIFTLSDSCRTGAKEAMAE 541

Query: 550  LRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPTAMIGD 609
            +RSLGIKT MLTGDSSAAALQAQKELGK LE VHAELLPEDKTRLINDFKREGPTAMIGD
Sbjct: 542  IRSLGIKTTMLTGDSSAAALQAQKELGKDLETVHAELLPEDKTRLINDFKREGPTAMIGD 601

Query: 610  GLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIENVI 669
            GLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAI+LARRANRKVIENVI
Sbjct: 602  GLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIRLARRANRKVIENVI 661

Query: 670  LSVVPRTAILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSATH 729
            LSV PRTAILGLAFGGHPLVWAAVL DVGACVLVILNSMLLLRGT+GHKGKKAG FSA+H
Sbjct: 662  LSVAPRTAILGLAFGGHPLVWAAVLADVGACVLVILNSMLLLRGTEGHKGKKAGMFSASH 721

Query: 730  CSSKHKCCHVGSHSDEHGSHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSLASGHHHHHHE 789
            CSSKHKCCHVGSHS+EHG HTHDHGC+N SSHSS+HH HH             HHHHHHE
Sbjct: 722  CSSKHKCCHVGSHSEEHGGHTHDHGCSNESSHSSSHHQHHH------------HHHHHHE 781

Query: 790  HKDCGSLKKTHDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAGNVKPCEHGHC 849
            H+DCGSL+KTH+GC  Q C+S CDSGMKN SSCKKSKLVDS S+ DD AG+VKP EH HC
Sbjct: 782  HEDCGSLRKTHNGCSTQKCASTCDSGMKNLSSCKKSKLVDSCSRADDPAGSVKPFEHEHC 841

Query: 850  VHNNQPDEHDHDAHLSCANHHVEDKHCSPKNTQESCSSQKCASNSCEKIKCTSSLASLDG 909
            VHNNQPDEH+H  H    N   E +HC   N  +    + C  N+             + 
Sbjct: 842  VHNNQPDEHEHCVH---NNQPDEHEHCVHNNQPD--EHEHCVHNNHPD--------EHEH 901

Query: 910  SVDSVELHESECCIHNSQSAQHDH--------EHDHHAHFSCADHHVEEKHCAPKNTQEC 969
             V +    E E C+HN+   +H+H        EH+HH HFSC DHHVE++HC+ KNT E 
Sbjct: 902  CVHNNRPDEHEHCVHNNHPDEHEHCVHDNQPDEHEHHTHFSCDDHHVEDEHCSLKNTLEF 961

Query: 970  CSSQKFASSSCEQI--------------SVELHGSGCCTHNSQSAQHDHDIHTLKCDLDD 1029
            CS  + AS+SCE+I              S ELH SGCCTHN+QSAQHDH+I TLKCDLDD
Sbjct: 962  CSFPRCASNSCEKIQCTSSPASLDGSAGSDELHESGCCTHNTQSAQHDHEIQTLKCDLDD 1021

Query: 1030 SHLSSPDHHIGHGCCSEKNAQKVSLSPQTCHSETCNLSPCGETKCVDSTARQHAPEGSLE 1089
            SH SSPDHH G+GCCS+KN+QKVSLS   CHSETCN SPCG+TKCVDST +QH PE SLE
Sbjct: 1022 SHSSSPDHHNGNGCCSQKNSQKVSLSHPMCHSETCNSSPCGKTKCVDSTEKQHTPEASLE 1081

Query: 1090 LLQDHKHCHQGSFDTSNFVLKSQEKHRKNCTGPCKSRSISVCTEDECRKRLEMIDDCAEA 1149
            LLQDH HCHQGSFDTSNFVL+SQE H K+C+GPCKSR +S CTEDEC +R EMI DC EA
Sbjct: 1082 LLQDHNHCHQGSFDTSNFVLESQENHGKSCSGPCKSRPLSRCTEDECTERAEMIVDCDEA 1141

Query: 1150 KGHHKMKQHHCDTHLSLRNEGTHPHCKTSNGDEGGAINTAISIKLD-SDHSNPKPASACK 1209
              HHKMKQHHC THLSL NEG HPHCK S GD  GAIN   +IKL+ +DHSNPK  + CK
Sbjct: 1142 NEHHKMKQHHCHTHLSLENEGVHPHCKASKGDNDGAINKTTNIKLEAADHSNPKHGNTCK 1201

Query: 1210 ALENRESNNSCKSCKRGSSQLKIGKSCAALEK-REYGGCCKSYMKECCRKHGDIRTAVRG 1253
            ALENRE+NN+CK+C+RGSSQLKIGK+CA L+K RE  GCCKSYM+ECCRKHGDIR AVRG
Sbjct: 1202 ALENRETNNNCKTCRRGSSQLKIGKTCAGLKKNREIRGCCKSYMRECCRKHGDIRMAVRG 1244

BLAST of Lag0033868 vs. ExPASy TrEMBL
Match: A0A6J1JIQ2 (cadmium/zinc-transporting ATPase HMA3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484805 PE=3 SV=1)

HSP 1 Score: 1922.1 bits (4978), Expect = 0.0e+00
Identity = 1008/1270 (79.37%), Postives = 1087/1270 (85.59%), Query Frame = 0

Query: 8    AEAAVAGKSGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHD 67
            A AA A  + GG LQ+SYFDVLGICC+SEIPLIENILKE+EG+KEI+VIVATRT+IV+H 
Sbjct: 4    AAAAEAPGNNGGALQKSYFDVLGICCSSEIPLIENILKEIEGIKEIKVIVATRTLIVLHH 63

Query: 68   NLLLSQAQIVKALNQARLEANVRAYGD-QKNHRKRWPSPYAVASGLLLLLSFLKYVNPIF 127
            NLL+SQAQIVKALNQARLEANVRAYGD QKNHRK+WPSPYAVASGLLLLLSFLKYVNP+F
Sbjct: 64   NLLVSQAQIVKALNQARLEANVRAYGDQQKNHRKKWPSPYAVASGLLLLLSFLKYVNPVF 123

Query: 128  RWVALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIA 187
            +WVALAAVAAGIWPI LKSF AVR+LRIDINILALIAVIGTIV+ DYLEAATIVFLFTIA
Sbjct: 124  KWVALAAVAAGIWPIALKSFTAVRHLRIDINILALIAVIGTIVLNDYLEAATIVFLFTIA 183

Query: 188  EWLESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIV 247
            EWLESRA HKANAVMSSLLSIAPQKAVLADTGEVVGADEVK+GTLLAVKAGEDIPIDGIV
Sbjct: 184  EWLESRAGHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKMGTLLAVKAGEDIPIDGIV 243

Query: 248  VEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE 307
            VEGKCEVDEKTLTGESFPV KQK+STVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE
Sbjct: 244  VEGKCEVDEKTLTGESFPVAKQKDSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE 303

Query: 308  AQNNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCA 367
            AQN+KSRTQRFID CAKFYTPAVIIIST IAVIP ALRL NRSHWFHLALVVLVSACPCA
Sbjct: 304  AQNSKSRTQRFIDKCAKFYTPAVIIISTGIAVIPFALRLHNRSHWFHLALVVLVSACPCA 363

Query: 368  LILSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALD 427
            LILSTPVASFCALTKAATSGLLIKGGD LETL KIKIMAFDKTGTITRGEFMVTEFQALD
Sbjct: 364  LILSTPVASFCALTKAATSGLLIKGGDCLETLGKIKIMAFDKTGTITRGEFMVTEFQALD 423

Query: 428  KDNISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRID 487
            K+NIS DTLLYWVSSIESKSSHPMAAALVDHGRSLSI PKPENVDDFQNFPGEGVHGRID
Sbjct: 424  KENISLDTLLYWVSSIESKSSHPMAAALVDHGRSLSINPKPENVDDFQNFPGEGVHGRID 483

Query: 488  GKDIYIGNRKIATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAM 547
            GKDIYIGNRKIATRANC TVP++ DEA+DGRT+GY+FC T+A G F+LSDSCRTGAKEAM
Sbjct: 484  GKDIYIGNRKIATRANCATVPEIKDEAKDGRTLGYVFCGTTAAGIFTLSDSCRTGAKEAM 543

Query: 548  DELRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPTAMI 607
             E+RSLGIKT MLTGDSSAAALQAQKELGKALE VHAELLPEDKTRLINDFKREGPTAMI
Sbjct: 544  AEIRSLGIKTTMLTGDSSAAALQAQKELGKALETVHAELLPEDKTRLINDFKREGPTAMI 603

Query: 608  GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIEN 667
            GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAI+LARRANRKVIEN
Sbjct: 604  GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIRLARRANRKVIEN 663

Query: 668  VILSVVPRTAILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSA 727
            VILSV PRTAILGLAFGGHPLVWAAVL DVGACVLVILNSMLLLRGT+GHKGKKAG FSA
Sbjct: 664  VILSVAPRTAILGLAFGGHPLVWAAVLADVGACVLVILNSMLLLRGTEGHKGKKAGMFSA 723

Query: 728  THCSSKHKCCHVGSHSDEHGSHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSLASGHHHHH 787
            +HCSSKHKCCHVGSHS+EHG HTHDHGC+N SSHSS+HH HH             HHHHH
Sbjct: 724  SHCSSKHKCCHVGSHSEEHGGHTHDHGCSNESSHSSSHHQHHH------------HHHHH 783

Query: 788  HEHKDCGSLKKTHDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAGNVKPCEHG 847
            HEH+DCGSLKKTH+GC  Q C+S CDSGMK SSSCKKSKLVDS S+ DD AG+VKP EH 
Sbjct: 784  HEHEDCGSLKKTHNGCSTQKCASTCDSGMKKSSSCKKSKLVDSCSRADDPAGSVKPFEHE 843

Query: 848  HCVHNNQPDEHDHDAHLSCANHHVEDKHCSPKNTQESCSSQKCASNSCEKIKCTSSLASL 907
            HCVHNNQP+EH+H  H +  + H +  H +  +  E C                      
Sbjct: 844  HCVHNNQPNEHEHCVHNNHPDEHEQCVHNNHPDEHEHC---------------------- 903

Query: 908  DGSVDSVELHESECCIHNSQSAQHDH--------EHDHHAHFSCADHHVEEKHCAPKNTQ 967
               V + +  E E C+HN+   +H H        EH+HH HFSC DHHVE++HC+ KNT 
Sbjct: 904  ---VHNNQPDEHEHCVHNNHPDEHGHCIHNNQPDEHEHHTHFSCDDHHVEDEHCSLKNTL 963

Query: 968  ECCSSQKFASSSCEQI--------------SVELHGSGCCTHNSQSAQHDHDIHTLKCDL 1027
            E CS  + AS+SCE+I              S ELH  GCCTHN+QSAQHDH+I TLKCDL
Sbjct: 964  EFCSFPRCASNSCEKIQCTSSPANLDGSAGSDELHERGCCTHNTQSAQHDHEIQTLKCDL 1023

Query: 1028 DDSHLSSPDHHIGHGCCSEKNAQKVSLSPQTCHSETCNLSPCGETKCVDSTARQHAPEGS 1087
            DDSH SSPDHH G+GCCS+KNAQKVSLS   CHSETCN SPCG+TKCVDST +QH P+GS
Sbjct: 1024 DDSHSSSPDHHNGNGCCSQKNAQKVSLSHSMCHSETCNSSPCGKTKCVDSTEKQHTPKGS 1083

Query: 1088 LELLQDHKHCHQGSFDTSNFVLKSQEKHRKNCTGPCKSRSISVCTEDECRKRLEMIDDCA 1147
            LELLQDH HCHQGS DTSNFV +SQE HRKNC+GPCKSR IS CTEDEC +R EMI DCA
Sbjct: 1084 LELLQDHNHCHQGSCDTSNFVSESQENHRKNCSGPCKSRPISRCTEDECTERAEMIVDCA 1143

Query: 1148 EAKGHHKMKQHHCDTHLSLRNEGTHPHCKTSNGDEGGAINTAISIKLD-SDHSNPKPASA 1207
            E   HHKMKQHHC THLSL NEG HPHCK S GD  GAIN    I+L+ +DHSNPK  + 
Sbjct: 1144 EGNEHHKMKQHHCHTHLSLENEGVHPHCKASKGDNDGAINKTTKIELEAADHSNPKHGNT 1203

Query: 1208 CKALENRESNNSCKSCKRGSSQLKIGKSCAALEK-REYGGCCKSYMKECCRKHGDIRTAV 1253
            CKALENRE+NN+CK+C+RGSSQLKIG++C  L+K RE  GCCKSYM+ECCRKHGDIR AV
Sbjct: 1204 CKALENRETNNNCKTCRRGSSQLKIGETCVGLKKNREIRGCCKSYMRECCRKHGDIRMAV 1236

BLAST of Lag0033868 vs. ExPASy TrEMBL
Match: A0A6J1JG42 (cadmium/zinc-transporting ATPase HMA3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484805 PE=3 SV=1)

HSP 1 Score: 1917.9 bits (4967), Expect = 0.0e+00
Identity = 1008/1270 (79.37%), Postives = 1081/1270 (85.12%), Query Frame = 0

Query: 8    AEAAVAGKSGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHD 67
            A AA A  + GG LQ+SYFDVLGICC+SEIPLIENILKE+EG+KEI+VIVATRT+IV+H 
Sbjct: 4    AAAAEAPGNNGGALQKSYFDVLGICCSSEIPLIENILKEIEGIKEIKVIVATRTLIVLHH 63

Query: 68   NLLLSQAQIVKALNQARLEANVRAYGD-QKNHRKRWPSPYAVASGLLLLLSFLKYVNPIF 127
            NLL+SQAQIVKALNQARLEANVRAYGD QKNHRK+WPSPYAVASGLLLLLSFLKYVNP+F
Sbjct: 64   NLLVSQAQIVKALNQARLEANVRAYGDQQKNHRKKWPSPYAVASGLLLLLSFLKYVNPVF 123

Query: 128  RWVALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIA 187
            +WVALAAVAAGIWPI LKSF AVR+LRIDINILALIAVIGTIV+ DYLEAATIVFLFTIA
Sbjct: 124  KWVALAAVAAGIWPIALKSFTAVRHLRIDINILALIAVIGTIVLNDYLEAATIVFLFTIA 183

Query: 188  EWLESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIV 247
            EWLESRA HKANAVMSSLLSIAPQKAVLADTGEVVGADEVK+GTLLAVKAGEDIPIDGIV
Sbjct: 184  EWLESRAGHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKMGTLLAVKAGEDIPIDGIV 243

Query: 248  VEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE 307
            VEGKCEVDEKTLTGESFPV KQK+STVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE
Sbjct: 244  VEGKCEVDEKTLTGESFPVAKQKDSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEE 303

Query: 308  AQNNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCA 367
            AQN+KSRTQRFID CAKFYTPAVIIIST IAVIP ALRL NRSHWFHLALVVLVSACPCA
Sbjct: 304  AQNSKSRTQRFIDKCAKFYTPAVIIISTGIAVIPFALRLHNRSHWFHLALVVLVSACPCA 363

Query: 368  LILSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALD 427
            LILSTPVASFCALTKAATSGLLIKGGD LETL KIKIMAFDKTGTITRGEFMVTEFQALD
Sbjct: 364  LILSTPVASFCALTKAATSGLLIKGGDCLETLGKIKIMAFDKTGTITRGEFMVTEFQALD 423

Query: 428  KDNISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRID 487
            K+NIS DTLLYWVSSIESKSSHPMAAALVDHGRSLSI PKPENVDDFQNFPGEGVHGRID
Sbjct: 424  KENISLDTLLYWVSSIESKSSHPMAAALVDHGRSLSINPKPENVDDFQNFPGEGVHGRID 483

Query: 488  GKDIYIGNRKIATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAM 547
            GKDIYIGNRKIATRANC TVP++ DEA+DGRT+GY+FC T+A G F+LSDSCRTGAKEAM
Sbjct: 484  GKDIYIGNRKIATRANCATVPEIKDEAKDGRTLGYVFCGTTAAGIFTLSDSCRTGAKEAM 543

Query: 548  DELRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPTAMI 607
             E+RSLGIKT MLTGDSSAAALQAQKELGKALE VHAELLPEDKTRLINDFKREGPTAMI
Sbjct: 544  AEIRSLGIKTTMLTGDSSAAALQAQKELGKALETVHAELLPEDKTRLINDFKREGPTAMI 603

Query: 608  GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIEN 667
            GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAI+LARRANRKVIEN
Sbjct: 604  GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIRLARRANRKVIEN 663

Query: 668  VILSVVPRTAILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSA 727
            VILSV PRTAILGLAFGGHPLVWAAVL DVGACVLVILNSMLLLRGT+GHKGKKAG FSA
Sbjct: 664  VILSVAPRTAILGLAFGGHPLVWAAVLADVGACVLVILNSMLLLRGTEGHKGKKAGMFSA 723

Query: 728  THCSSKHKCCHVGSHSDEHGSHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSLASGHHHHH 787
            +HCSSKHKCCHVGSHS+EHG HTHDHGC+N SSHSS+HH HH             HHHHH
Sbjct: 724  SHCSSKHKCCHVGSHSEEHGGHTHDHGCSNESSHSSSHHQHHH------------HHHHH 783

Query: 788  HEHKDCGSLKKTHDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAGNVKPCEHG 847
            HEH+DCGSLKKTH+GC  Q C+S CDSGMK SSSCKKSKLVDS S+ DD AG+VKP EH 
Sbjct: 784  HEHEDCGSLKKTHNGCSTQKCASTCDSGMKKSSSCKKSKLVDSCSRADDPAGSVKPFEHE 843

Query: 848  HCVHNNQPDEHDHDAHLSCANHHVEDKHCSPKNTQESCSSQKCASNSCEKIKCTSSLASL 907
            HCVHNN PDEH+   H    NH  E +HC   N  +                        
Sbjct: 844  HCVHNNHPDEHEQCVH---NNHPDEHEHCVHNNQPD------------------------ 903

Query: 908  DGSVDSVELHESECCIHNSQSAQHDH--------EHDHHAHFSCADHHVEEKHCAPKNTQ 967
                      E E C+HN+   +H H        EH+HH HFSC DHHVE++HC+ KNT 
Sbjct: 904  ----------EHEHCVHNNHPDEHGHCIHNNQPDEHEHHTHFSCDDHHVEDEHCSLKNTL 963

Query: 968  ECCSSQKFASSSCEQI--------------SVELHGSGCCTHNSQSAQHDHDIHTLKCDL 1027
            E CS  + AS+SCE+I              S ELH  GCCTHN+QSAQHDH+I TLKCDL
Sbjct: 964  EFCSFPRCASNSCEKIQCTSSPANLDGSAGSDELHERGCCTHNTQSAQHDHEIQTLKCDL 1023

Query: 1028 DDSHLSSPDHHIGHGCCSEKNAQKVSLSPQTCHSETCNLSPCGETKCVDSTARQHAPEGS 1087
            DDSH SSPDHH G+GCCS+KNAQKVSLS   CHSETCN SPCG+TKCVDST +QH P+GS
Sbjct: 1024 DDSHSSSPDHHNGNGCCSQKNAQKVSLSHSMCHSETCNSSPCGKTKCVDSTEKQHTPKGS 1083

Query: 1088 LELLQDHKHCHQGSFDTSNFVLKSQEKHRKNCTGPCKSRSISVCTEDECRKRLEMIDDCA 1147
            LELLQDH HCHQGS DTSNFV +SQE HRKNC+GPCKSR IS CTEDEC +R EMI DCA
Sbjct: 1084 LELLQDHNHCHQGSCDTSNFVSESQENHRKNCSGPCKSRPISRCTEDECTERAEMIVDCA 1143

Query: 1148 EAKGHHKMKQHHCDTHLSLRNEGTHPHCKTSNGDEGGAINTAISIKLD-SDHSNPKPASA 1207
            E   HHKMKQHHC THLSL NEG HPHCK S GD  GAIN    I+L+ +DHSNPK  + 
Sbjct: 1144 EGNEHHKMKQHHCHTHLSLENEGVHPHCKASKGDNDGAINKTTKIELEAADHSNPKHGNT 1203

Query: 1208 CKALENRESNNSCKSCKRGSSQLKIGKSCAALEK-REYGGCCKSYMKECCRKHGDIRTAV 1253
            CKALENRE+NN+CK+C+RGSSQLKIG++C  L+K RE  GCCKSYM+ECCRKHGDIR AV
Sbjct: 1204 CKALENRETNNNCKTCRRGSSQLKIGETCVGLKKNREIRGCCKSYMRECCRKHGDIRMAV 1224

BLAST of Lag0033868 vs. ExPASy TrEMBL
Match: A0A6J1C1P2 (cadmium/zinc-transporting ATPase HMA3-like OS=Momordica charantia OX=3673 GN=LOC111007462 PE=3 SV=1)

HSP 1 Score: 1698.3 bits (4397), Expect = 0.0e+00
Identity = 916/1253 (73.10%), Postives = 1005/1253 (80.21%), Query Frame = 0

Query: 16   SGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHDNLLLSQAQ 75
            +   GLQRSYFDVLGICC+SEIP++ENILK +EGV+E+ VIVATRTVIVVHDN L+SQ Q
Sbjct: 2    AAAAGLQRSYFDVLGICCSSEIPVVENILKAIEGVREVTVIVATRTVIVVHDN-LVSQTQ 61

Query: 76   IVKALNQARLEANVRAYGDQKNHRKRWPSPYAVASGLLLLLSFLKYVNPIFRWVALAAVA 135
            IVKALNQARLEANVRAYGDQK+HRK+WPSPYAVASGLLLLLSFLKYVNPIF+W+ALAAVA
Sbjct: 62   IVKALNQARLEANVRAYGDQKDHRKKWPSPYAVASGLLLLLSFLKYVNPIFQWLALAAVA 121

Query: 136  AGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIAEWLESRASH 195
            AGIWPI LKSFAAVRNLR+DINILALIAVIGTIVMKDYLEAAT+VFLFTIAEWLESRASH
Sbjct: 122  AGIWPIALKSFAAVRNLRLDINILALIAVIGTIVMKDYLEAATVVFLFTIAEWLESRASH 181

Query: 196  KANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIVVEGKCEVDE 255
            KANAVMSSLLSIAPQKAVLAD+GEVV ADEVKLGTLLAVK GEDIPIDGIV EG CEVDE
Sbjct: 182  KANAVMSSLLSIAPQKAVLADSGEVVAADEVKLGTLLAVKPGEDIPIDGIVEEGNCEVDE 241

Query: 256  KTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQ 315
            KTLTGESFPVPKQK+STVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQ
Sbjct: 242  KTLTGESFPVPKQKSSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQ 301

Query: 316  RFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCALILSTPVAS 375
            RFID CAKFYTP VIIISTCIAVIP ALRLPNRSHWFHLALVVLVSACPCALILSTPVAS
Sbjct: 302  RFIDKCAKFYTPGVIIISTCIAVIPAALRLPNRSHWFHLALVVLVSACPCALILSTPVAS 361

Query: 376  FCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALDKDNISFDTL 435
            FCALTKAATSGLLIKGGD+LETLAKIK+MAFDKTGTITRGEF+VTEFQALD+D+IS +TL
Sbjct: 362  FCALTKAATSGLLIKGGDYLETLAKIKVMAFDKTGTITRGEFVVTEFQALDEDDISLNTL 421

Query: 436  LYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRIDGKDIYIGNR 495
            LYWVSSIESKSSHPMAAALVDHGRSLSI PKPENVDDFQNFPGEGVHGRIDGKDIYIGNR
Sbjct: 422  LYWVSSIESKSSHPMAAALVDHGRSLSIDPKPENVDDFQNFPGEGVHGRIDGKDIYIGNR 481

Query: 496  KIATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAMDELRSLGIK 555
            KIA RANC TVP++ D+A+DGRTVGYIFC T+A G FSLSDSCRTGAKEAM+ELRSLGIK
Sbjct: 482  KIAIRANCPTVPEIKDDAKDGRTVGYIFCGTTAAGMFSLSDSCRTGAKEAMEELRSLGIK 541

Query: 556  TAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPTAMIGDGLNDAPA 615
            TAMLTGDSSAAALQAQKELGKALE VHAELLPEDKTRLINDFKREGPTAMIGDGLNDAPA
Sbjct: 542  TAMLTGDSSAAALQAQKELGKALETVHAELLPEDKTRLINDFKREGPTAMIGDGLNDAPA 601

Query: 616  LATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIENVILSVVPRT 675
            LATADIGISMGISGSALAIETGDVILM+NDIRKIPKAI+LAR+AN KVIENVILSV+PRT
Sbjct: 602  LATADIGISMGISGSALAIETGDVILMSNDIRKIPKAIRLARKANMKVIENVILSVIPRT 661

Query: 676  AILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSATHCSSKHKC 735
            AILGLAF GHPLVWAAVL DVGACVLVILNSMLLLRGT   KGKKAGKFSATHCSSKH+C
Sbjct: 662  AILGLAFAGHPLVWAAVLADVGACVLVILNSMLLLRGTSERKGKKAGKFSATHCSSKHEC 721

Query: 736  CHVGSHSDEHGSHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSLASGHHHHHHEHKDCGSL 795
            CHV SHSD+HG+H+ D GCN+ +SHS                    HHHHHH H+D  S 
Sbjct: 722  CHVSSHSDQHGNHSRDLGCNHENSHS--------------------HHHHHHVHEDHNSH 781

Query: 796  KKTHDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAGNVKPCEHGHCVHNNQPD 855
            KKTHDGCLPQNC+S CDSG  NSSSCKKSKLVDSSSK+DDSAG+V+P EH HCVH NQ  
Sbjct: 782  KKTHDGCLPQNCASTCDSGKTNSSSCKKSKLVDSSSKLDDSAGSVRPYEHEHCVH-NQSA 841

Query: 856  EHDHDAHLSCANHHVEDKHCSPKNTQESCSSQKCASN----SCEKIKCTSSLASLDGSVD 915
            +HDH  H SC +HH+ED HCSP+NTQE CSS  CASN     CEK +CT+S +SLDGS  
Sbjct: 842  QHDHHTHSSCTDHHIEDTHCSPENTQEFCSSWDCASNCQSSKCEKTECTNSPSSLDGSAG 901

Query: 916  SVELHESECCIHNSQSAQHDHEHDHHAHFSCADHHVEEKHCAPKNTQECCSSQKFASSSC 975
            S+E HE                                                      
Sbjct: 902  SIENHE------------------------------------------------------ 961

Query: 976  EQISVELHGSGCCTHNSQSAQHDHDIHTLKCDLDDSHLSSPDHHIGHGCCSEKNAQKVSL 1035
                     SGCCTHNS++AQH+H+I   KC+ ++SH+S+ DHHI  GCCS KN QKVSL
Sbjct: 962  ---------SGCCTHNSRAAQHNHEIQIPKCNSENSHMSNLDHHIDDGCCSHKNTQKVSL 1021

Query: 1036 SPQTCHSETCNLSPCGETKCVDSTARQHAPEGSLELLQDHKHCHQGSFDTSNFVLKSQEK 1095
                CHS+T   +PCG+TKC DSTARQ    GSLELLQD                   +K
Sbjct: 1022 PHSKCHSKTYISNPCGKTKCADSTARQDGSSGSLELLQD-------------------QK 1081

Query: 1096 HRKNCTGPCKSRSISVCTEDECRKRLEMIDDCAEAKGHHKMKQHHCDTHLSLRN------ 1155
             +KN            CT+DEC KR+ MID CA+AKGH ++K HHC+TH   +N      
Sbjct: 1082 IKKN------------CTKDECNKRVAMIDGCAKAKGHLEIK-HHCNTHFFEKNGTSNRD 1136

Query: 1156 --EGTHPHCKTSNGDEGGAINTAISIKLDSDHSNPKPASACKALENRESNNSCKSCKRGS 1215
              EG HP  +  NGD  G+INT I ++L++DH + KPA+ CK LE RE+ +SCKSCK   
Sbjct: 1142 GHEGAHPDSEAWNGDSSGSINTTI-VELEADHLHSKPANTCKPLEKRETGDSCKSCKVEC 1136

Query: 1216 SQLKIGKSCAALEKREYGGCCKSYMKEC----CRKHGDIRTAVRGGLNEIVIE 1253
            S+LK+ + C +LEKR  GGCCKSY KEC    CR+H DI T +RGGL EI+IE
Sbjct: 1202 SELKLKQCCPSLEKRGMGGCCKSYTKECCRRRCRRHSDIGTTLRGGLKEIIIE 1136

BLAST of Lag0033868 vs. ExPASy TrEMBL
Match: A0A5A7USU1 (Cadmium/zinc-transporting ATPase HMA3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005520 PE=3 SV=1)

HSP 1 Score: 1646.7 bits (4263), Expect = 0.0e+00
Identity = 928/1364 (68.04%), Postives = 1001/1364 (73.39%), Query Frame = 0

Query: 1    MAAGEEKAEAAVAGKSGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATR 60
            MAA      A  AG   GGGL++SYFDVLGICC+SEIP+IENILKE+EG+KEIRVIVATR
Sbjct: 1    MAATAASTAATEAGGKKGGGLKKSYFDVLGICCSSEIPVIENILKEIEGIKEIRVIVATR 60

Query: 61   TVIVVHDNLLLSQAQIVKALNQARLEANVRAYGDQKNHRKRWPSPYAVASGLLLLLSFLK 120
            TVIV+HD+LL+SQAQIVKALNQAR EANVRAYGDQK+HRK+WPSPYAVASGLLLLLS LK
Sbjct: 61   TVIVLHDDLLVSQAQIVKALNQARFEANVRAYGDQKDHRKKWPSPYAVASGLLLLLSLLK 120

Query: 121  YVNPIFRWVALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIV 180
            YVNPIFRWVALAAVAAGI PIVLKSFAAVR+LRIDINILALIAVIGTIV+KDYLEAATIV
Sbjct: 121  YVNPIFRWVALAAVAAGILPIVLKSFAAVRHLRIDINILALIAVIGTIVLKDYLEAATIV 180

Query: 181  FLFTIAEWLESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDI 240
            FLFTIAEWLESRA+HKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDI
Sbjct: 181  FLFTIAEWLESRAAHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDI 240

Query: 241  PIDGIVVEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKM 300
            PIDGIVVEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKM
Sbjct: 241  PIDGIVVEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKM 300

Query: 301  AKLVEEAQNNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLV 360
            AKLVEEAQNNKSRTQRFID CAKFYTPAVIIISTCI VIPVALRLPNRSHWFHLALVVLV
Sbjct: 301  AKLVEEAQNNKSRTQRFIDKCAKFYTPAVIIISTCIVVIPVALRLPNRSHWFHLALVVLV 360

Query: 361  SACPCALILSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVT 420
            SACPCALILSTPVASFCALTKAATSGLLIKGGD+LETL KIKIMAFDKTGTITRGEFMVT
Sbjct: 361  SACPCALILSTPVASFCALTKAATSGLLIKGGDYLETLGKIKIMAFDKTGTITRGEFMVT 420

Query: 421  EFQALDKDNISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEG 480
            EFQ LDKDNIS DTLLYWVSSIESKSSHPMAAALVDHGRSLSI PKPENVDDFQNFPGEG
Sbjct: 421  EFQVLDKDNISLDTLLYWVSSIESKSSHPMAAALVDHGRSLSIDPKPENVDDFQNFPGEG 480

Query: 481  VHGRIDGKDIYIGNRKIATRANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRT 540
            VHGRIDGKDIYIGNRKIATRANC TVP++ DEA+DGRTVGYIFC T A G FSLSDSCRT
Sbjct: 481  VHGRIDGKDIYIGNRKIATRANCATVPEIKDEAKDGRTVGYIFCGTIAAGVFSLSDSCRT 540

Query: 541  GAKEAMDELRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKRE 600
            GAKEAMDELRSLGIKTAMLTGDSSAAALQAQKELGKAL+ VHAELLP+DKTRLINDFK+E
Sbjct: 541  GAKEAMDELRSLGIKTAMLTGDSSAAALQAQKELGKALQTVHAELLPQDKTRLINDFKKE 600

Query: 601  GPTAMIGDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRAN 660
            GPTAMIGDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRK+PKAI+LARRAN
Sbjct: 601  GPTAMIGDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKVPKAIRLARRAN 660

Query: 661  RKVIENVILSVVPRTAILGLAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKK 720
             KVIENVILSV PR AILGLAFGGHPLVWAAVL DVGACVLVILNSMLLLRGTDGHKGKK
Sbjct: 661  NKVIENVILSVAPRIAILGLAFGGHPLVWAAVLADVGACVLVILNSMLLLRGTDGHKGKK 720

Query: 721  AGKFSATHCSSKHKCCHVGSHSDEHG--SHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSL 780
            AGKFSATHCSSKHKCCHV SHSDEH   +H HDHGCN+ SSHSS HHHHH          
Sbjct: 721  AGKFSATHCSSKHKCCHVSSHSDEHSGHAHAHDHGCNDHSSHSSRHHHHHH--------- 780

Query: 781  ASGHHHHHHEHKDCGSLKKTHDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAG 840
               HHHHH E +DCGSLKKTHDGCL QN +S CDS +KNSSSCKKSKL++ SSK+D S G
Sbjct: 781  ---HHHHHDEQEDCGSLKKTHDGCLQQNHASMCDSKLKNSSSCKKSKLMNPSSKIDGSTG 840

Query: 841  NVKPCEHGHC------VHNNQPDEHDHDAHLSCANHHVEDKH--------CSPKNTQESC 900
             VK  EH H          +    H H  H S  +H  ED H        C P+N    C
Sbjct: 841  CVKLREHDHTHDHGCNSDGSDSSSHSHHHHHSHHHHEHEDCHSLKKTHEVCVPQNHASKC 900

Query: 901  SSQKCASNSCEKIKCTSSLASLDGSVDSVELHESE------CCIHNSQSAQHDH------ 960
             S+   S+ C K K   S + ++GS  SV+L+E +      C I ++ S+ H H      
Sbjct: 901  DSRSKYSSLCNKSKLVDSCSKVNGSTGSVQLYEHDHTHDHGCNIDSTDSSSHSHHNHHHH 960

Query: 961  ------------------------------------------------------------ 1020
                                                                        
Sbjct: 961  HHEHEDCGSLKKTNDSCLLQNCASKCDSGLKSSSSCKKSELVDSSSKVDDSAGSLKPCEH 1020

Query: 1021 ---------EHDHHAHFSCADHHVEEKHCAPKNTQECCSSQKFASSSCEQIS-------- 1080
                     EHDHHA+FSCADHH ++  C+P+NTQE CS QK AS+SCE I         
Sbjct: 1021 GHVHNDQPAEHDHHAYFSCADHHAKDVLCSPENTQEFCSFQKCASNSCETIKCTSSPASH 1080

Query: 1081 ------VELHGSGCCTHNSQSAQHDHDIHTLKCDLDDSHLSSPDHHIGHGCCSEKNAQKV 1140
                  VEL   GCCTHN+QSAQHDHDI + KCD DDSH  S +HHI + CCS+KN QKV
Sbjct: 1081 DESAVIVELEECGCCTHNTQSAQHDHDIQSPKCDFDDSHSPSLEHHISNDCCSQKNTQKV 1140

Query: 1141 SLSPQTCHSETCNLSPCGETKCVDSTARQHAPEGSLELLQDHKHCHQGSFDTSNFVLKSQ 1200
            S+S     SETC                                                
Sbjct: 1141 SISHPMRDSETC------------------------------------------------ 1200

Query: 1201 EKHRKNCTGPCKSRSISVCTEDECRKRLEMIDDCAEAKGHHKMKQHHCDTHLSLRNEGTH 1253
                                                                    EG H
Sbjct: 1201 -------------------------------------------------------KEGVH 1248

BLAST of Lag0033868 vs. TAIR 10
Match: AT4G30110.1 (heavy metal atpase 2 )

HSP 1 Score: 925.6 bits (2391), Expect = 4.2e-269
Identity = 548/979 (55.98%), Postives = 681/979 (69.56%), Query Frame = 0

Query: 21  LQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHDNLLLSQAQIVKAL 80
           + +SYFDVLGICC SE+PLIENIL  M+GVKE  VIV +RTVIVVHD L+LSQ QIVKAL
Sbjct: 6   MTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQIVKAL 65

Query: 81  NQARLEANVRAYGDQKNHRKRWPSPYAVASGLLLLLSFLKYVNPIFRWVALAAVAAGIWP 140
           NQA+LEANVR  G + N + +WPSP+AV SG+LLLLSF KY+   FRW+A+AAV AGI+P
Sbjct: 66  NQAQLEANVRVTG-ETNFKNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVVAGIYP 125

Query: 141 IVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIAEWLESRASHKANAV 200
           I+ K+ A++   RIDINIL ++ V  TI M+DY EAA +VFLFTIAEWL+SRAS+KA+AV
Sbjct: 126 ILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASYKASAV 185

Query: 201 MSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIVVEGKCEVDEKTLTG 260
           M SL+S+APQKAV+A+TGE V  DE+K  T++AVKAGE IPIDG+VV+G CEVDEKTLTG
Sbjct: 186 MQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDEKTLTG 245

Query: 261 ESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQRFIDI 320
           E+FPVPK K+STVWAGTINLNGY+TV TTALAEDCVVAKMAKLVEEAQN+K+ TQRFID 
Sbjct: 246 EAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQRFIDK 305

Query: 321 CAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCALILSTPVASFCALT 380
           C+K+YTPA+I+IS C   IP AL++ N  HW HLALVVLVSACPC LILSTPVA+FCALT
Sbjct: 306 CSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVATFCALT 365

Query: 381 KAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALDKDNISFDTLLYWVS 440
           KAATSGLLIKG D+LETLAKIKI+AFDKTGTITRGEF+V +FQ+L +D IS  +LLYWVS
Sbjct: 366 KAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSLSED-ISLQSLLYWVS 425

Query: 441 SIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRIDGKDIYIGNRKIATR 500
           S ESKSSHPMAAA+VD+ RS+S+ PKPE V+D+QNFPGEG++G+IDGK++YIGN++IA+R
Sbjct: 426 STESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRIASR 485

Query: 501 ANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAMDELRSLGIKTAMLT 560
           A C +VPD++ + + G+T+GY++   +  G F+LSD+CR+G  +AM EL+SLGIK AMLT
Sbjct: 486 AGCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLGIKIAMLT 545

Query: 561 GDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKR-EGPTAMIGDGLNDAPALATA 620
           GD+ AAA+ AQ++LG A++IV AELLPEDK+ +I   KR EGPTAM+GDGLNDAPALATA
Sbjct: 546 GDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMVGDGLNDAPALATA 605

Query: 621 DIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIENVILSVVPRTAILG 680
           DIGISMG+SGSALA ETG++ILM+NDIR+IP+AIKLA+RA RKV+ENV++S+  + AIL 
Sbjct: 606 DIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKGAILA 665

Query: 681 LAFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHK---------------------G 740
           LAF GHPL+WAAVL DVG C+LVILNSMLLL  +D HK                     G
Sbjct: 666 LAFAGHPLIWAAVLADVGTCLLVILNSMLLL--SDKHKTGNKCYRESSSSSVLIAEKLEG 725

Query: 741 KKAG--------KFSATHCSSKHKCCHVGSHSDE------HGSHTHDH-GC--------- 800
             AG        K S  HC  K  CC  G+ + E        S  H H GC         
Sbjct: 726 DAAGDMEAGLLPKISDKHC--KPGCC--GTKTQEKAMKPAKASSDHSHSGCCETKQKDNV 785

Query: 801 ----NNGSSHSSNHHHHHDHGCNNDSSLASGHHHHHHEHKDC-------------GSLKK 860
                +  +   +  H HD GC  D S    H H     + C             G  ++
Sbjct: 786 TVVKKSCCAEPVDLGHGHDSGCCGDKS-QQPHQHEVQVQQSCHNKPSGLDSGCCGGKSQQ 845

Query: 861 THDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAGNVKPCEHGHCVHNNQPDEH 920
            H   L Q+C  K  SG+   +  K        +   D+   +K   +G C         
Sbjct: 846 PHQHELQQSCHDK-PSGLDIGTGPKHEGSSTLVNLEGDAKEELKVLVNGFC--------- 905

Query: 921 DHDAHLSCANHHVE-DKHCSPKNTQESCSSQKCASNSCEKIKCTSSLASLDGSVDSVELH 935
              A L+  +  V+ D HC     + +CSS++         +C                H
Sbjct: 906 SSPADLAITSLKVKSDSHC-----KSNCSSRE---------RCH---------------H 936

BLAST of Lag0033868 vs. TAIR 10
Match: AT2G19110.1 (heavy metal atpase 4 )

HSP 1 Score: 924.1 bits (2387), Expect = 1.2e-268
Identity = 565/1173 (48.17%), Postives = 765/1173 (65.22%), Query Frame = 0

Query: 21   LQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATRTVIVVHDNLLLSQAQIVKAL 80
            LQ+SYFDVLGICC SE+P+IENILK ++GVKE  VIV +RTVIVVHD+LL+S  QI KAL
Sbjct: 16   LQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSLLISPFQIAKAL 75

Query: 81   NQARLEANVRAYGDQKNHRKRWPSPYAVASGLLLLLSFLKYVNPIFRWVALAAVAAGIWP 140
            N+ARLEANVR  G + + + +WPSP+AV SGLLLLLSFLK+V    RW+A+AAVAAGI+P
Sbjct: 76   NEARLEANVRVNG-ETSFKNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLAVAAVAAGIYP 135

Query: 141  IVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIVFLFTIAEWLESRASHKANAV 200
            I+ K+FA+++  RIDINIL +I VI T+ M+D++EAA +VFLFTI++WLE+RAS+KA +V
Sbjct: 136  ILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLETRASYKATSV 195

Query: 201  MSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDIPIDGIVVEGKCEVDEKTLTG 260
            M SL+S+APQKA++A+TGE V  DEVK+ T++AVKAGE IPIDGIVV+G CEVDEKTLTG
Sbjct: 196  MQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGNCEVDEKTLTG 255

Query: 261  ESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQRFIDI 320
            E+FPVPKQ++STVWAGTINLNGY+ VKTT+LA DCVVAKMAKLVEEAQ++K+++QR ID 
Sbjct: 256  EAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSSKTKSQRLIDK 315

Query: 321  CAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLVSACPCALILSTPVASFCALT 380
            C+++YTPA+I++S C+A++PV +++ N  HWFHLALVVLVS CPC LILSTPVA+FCALT
Sbjct: 316  CSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILSTPVATFCALT 375

Query: 381  KAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALDKDNISFDTLLYWVS 440
            KAATSGLLIK  D+L+TL+KIKI+AFDKTGTITRGEF+V +F++L +D I+  +LLYWVS
Sbjct: 376  KAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSLSRD-INLRSLLYWVS 435

Query: 441  SIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEGVHGRIDGKDIYIGNRKIATR 500
            S+ESKSSHPMAA +VD+ +S+S+ P+PE V+D+QNFPGEG++G+IDG DI+IGN+KIA+R
Sbjct: 436  SVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFIGNKKIASR 495

Query: 501  ANCTTVPDMNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAMDELRSLGIKTAMLT 560
            A C+TVP++  + + G+TVGY++      G F+LSD+CR+G  +AM EL+SLGIKTAMLT
Sbjct: 496  AGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKSLGIKTAMLT 555

Query: 561  GDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPTAMIGDGLNDAPALATAD 620
            GD+ AAA+ AQ++LG  L++VH +LLPEDK+R+I +FK+EGPTAM+GDG+NDAPALATAD
Sbjct: 556  GDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKEGPTAMVGDGVNDAPALATAD 615

Query: 621  IGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIENVILSVVPRTAILGL 680
            IGISMGISGSALA +TG++ILM+NDIR+IP+A+KLARRA RKV+ENV LS++ +  IL L
Sbjct: 616  IGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLSIILKAGILAL 675

Query: 681  AFGGHPLVWAAVLVDVGACVLVILNSMLLLRGTDGHKGKKAGKFSATHCSSKHKCCHVGS 740
            AF GHPL+WAAVLVDVG C+LVI NSMLLLR       KK  + S +  + +        
Sbjct: 676  AFAGHPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKKCYRASTSKLNGRKLEGDDDY 735

Query: 741  HSD-EHGSHTHDHGCNNGSSHSSNHHHHHDHGCNNDSSLASGHHHHHHEHKDCGSLKK-- 800
              D E G  T        SS   +  +  +      SS  S  H H      CG  K+  
Sbjct: 736  VVDLEAGLLTKSGNGQCKSSCCGDKKNQENVVMMKPSSKTSSDHSH---PGCCGDKKEEK 795

Query: 801  ----THDGCLPQNCSSKCDSGMKNSSSCKKSKLVDSSSKVDDSAG--------------- 860
                  DGC  +  + K +  M + SSCKKS  V    K+   +G               
Sbjct: 796  VKPLVKDGCCSEK-TRKSEGDMVSLSSCKKSSHVKHDLKMKGGSGCCASKNEKGKEVVAK 855

Query: 861  -----------NVKPCEHGHCVHNNQPDEHDHDAH-LSCANHHVEDKHCSPKNTQESCSS 920
                       +V  C+ GHC    Q ++       +  A  HVE +      T+E+C +
Sbjct: 856  SCCEKPKQQVESVGDCKSGHCEKKKQAEDIVVPVQIIGHALTHVEIE----LQTKETCKT 915

Query: 921  QKCASNSCEKIKCTSSLASLDGS---VDSVELHESECCIHNSQSAQHDHEHDHHAHFS-- 980
              C  +S EK+K T  L S + +      V + +   C   S++     +  H    S  
Sbjct: 916  SCC--DSKEKVKETGLLLSSENTPYLEKGVLIKDEGNCKSGSENMGTVKQSCHEKGCSDE 975

Query: 981  --------CADHHVEEKHCAP-----KNTQECCSSQKFASSSCEQISVELHGSGCCTHNS 1040
                     ++   +++ C+      + T +    +K  S   E+  +++    CC    
Sbjct: 976  KQTGEITLASEEETDDQDCSSGCCVNEGTVKQSFDEKKHSVLVEKEGLDMETGFCCDAKL 1035

Query: 1041 QSAQHDHDIHTLKCDLDDSHLSSPDHHIGHGCCSE--KNAQKVSLSPQTCHSETCNLSPC 1100
                +       +C L+       + H   GCC E  +  +   +S +   S  C+   C
Sbjct: 1036 VCCGNTEGEVKEQCRLE----IKKEEHCKSGCCGEEIQTGEITLVSEEETESTNCSTGCC 1095

Query: 1101 GETKCVDSTARQHAPE---GSLELLQDHKHCHQGSFDTSNFVLKSQEKHRKNCTGPCKSR 1137
             + + V  T  +         LE+ +D +HC + S          + K  + C   C+ R
Sbjct: 1096 VDKEEVTQTCHEKPASLVVSGLEVKKD-EHC-ESSHRAVKVETCCKVKIPEACASKCRDR 1155

BLAST of Lag0033868 vs. TAIR 10
Match: AT4G30120.1 (heavy metal atpase 3 )

HSP 1 Score: 676.0 bits (1743), Expect = 5.7e-194
Identity = 347/553 (62.75%), Postives = 441/553 (79.75%), Query Frame = 0

Query: 1   MAAGEEKAEAAVAGKSGGGGLQRSYFDVLGICCASEIPLIENILKEMEGVKEIRVIVATR 60
           MA GEE  +           LQ SYFDV+GICC+SE+ ++ N+L++++GVKE  VIV +R
Sbjct: 1   MAEGEESKKM---------NLQTSYFDVVGICCSSEVSIVGNVLRQVDGVKEFSVIVPSR 60

Query: 61  TVIVVHDNLLLSQAQIVKALNQARLEANVRAYGDQKNHRKRWPSPYAVASGLLLLLSFLK 120
           TVIVVHD  L+S  QIVKALNQARLEA+VR YG + + + +WPSP+A+ SG+LL+LSF K
Sbjct: 61  TVIVVHDTFLISPLQIVKALNQARLEASVRPYG-ETSLKSQWPSPFAIVSGVLLVLSFFK 120

Query: 121 YVNPIFRWVALAAVAAGIWPIVLKSFAAVRNLRIDINILALIAVIGTIVMKDYLEAATIV 180
           Y      W+A+ AV AG++PI+ K+ A+V   R+DIN L LIAVI T+ M+D+ EAATIV
Sbjct: 121 YFYSPLEWLAIVAVVAGVFPILAKAVASVTRFRLDINALTLIAVIATLCMQDFTEAATIV 180

Query: 181 FLFTIAEWLESRASHKANAVMSSLLSIAPQKAVLADTGEVVGADEVKLGTLLAVKAGEDI 240
           FLF++A+WLES A+HKA+ VMSSL+S+AP+KAV+ADTG  V  DEV + T+++VKAGE I
Sbjct: 181 FLFSVADWLESSAAHKASIVMSSLMSLAPRKAVIADTGLEVDVDEVGINTVVSVKAGESI 240

Query: 241 PIDGIVVEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGYVTVKTTALAEDCVVAKM 300
           PIDG+VV+G C+VDEKTLTGESFPV KQ+ STV A TINLNGY+ VKTTALA DCVVAKM
Sbjct: 241 PIDGVVVDGSCDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTTALARDCVVAKM 300

Query: 301 AKLVEEAQNNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVALRLPNRSHWFHLALVVLV 360
            KLVEEAQ ++++TQRFID C+++YTPAV++ + C AVIPV L++ + SHWFHLALVVLV
Sbjct: 301 TKLVEEAQKSQTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVLV 360

Query: 361 SACPCALILSTPVASFCALTKAATSGLLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVT 420
           S CPC LILSTPVA+FCALTKAATSG LIK GD LETLAKIKI+AFDKTGTIT+ EFMV+
Sbjct: 361 SGCPCGLILSTPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMVS 420

Query: 421 EFQALDKDNISFDTLLYWVSSIESKSSHPMAAALVDHGRSLSIFPKPENVDDFQNFPGEG 480
           +F++L   +I+   LLYWVSSIE KSSHPMAAAL+D+ RS+S+ PKP+ V++FQNFPGEG
Sbjct: 421 DFRSL-SPSINLHKLLYWVSSIECKSSHPMAAALIDYARSVSVEPKPDIVENFQNFPGEG 480

Query: 481 VHGRIDGKDIYIGNRKIATRANCTT--VPDMNDEARDGRTVGYIFCETSAVGTFSLSDSC 540
           V+GRIDG+DIYIGN++IA RA C T  VPD+    + G+T+GYI+      G+F+L D C
Sbjct: 481 VYGRIDGQDIYIGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLLDGC 540

Query: 541 RTGAKEAMDELRS 552
           R G  +A+ EL+S
Sbjct: 541 RYGVAQALKELKS 542

BLAST of Lag0033868 vs. TAIR 10
Match: AT5G44790.1 (copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPase (RAN1) )

HSP 1 Score: 268.9 bits (686), Expect = 2.1e-71
Identity = 196/592 (33.11%), Postives = 310/592 (52.36%), Query Frame = 0

Query: 173 YLEAATIVFLFT-IAEWLESRASHKANAVMSSLLSIAPQKAVL---ADTGEVVGADE--- 232
           Y +A+ ++  F  + ++LES A  K +  M  L+ + P  A+L      G++VG  E   
Sbjct: 402 YFDASAMLITFVLLGKYLESLAKGKTSDAMKKLVQLTPATAILLTEGKGGKLVGEREIDA 461

Query: 233 --VKLGTLLAVKAGEDIPIDGIVVEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNGY 292
             ++ G  L V  G  IP DG+VV G   V+E  +TGES PV K+ +S V  GTIN++G 
Sbjct: 462 LLIQPGDTLKVHPGAKIPADGVVVWGSSYVNESMVTGESVPVSKEVDSPVIGGTINMHGA 521

Query: 293 VTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQRFIDICAKFYTPAVII----------IS 352
           + +K T +  D V++++  LVE AQ +K+  Q+F D  A  + P VI           I 
Sbjct: 522 LHMKATKVGSDAVLSQIISLVETAQMSKAPIQKFADYVASIFVPVVITLALFTLVGWSIG 581

Query: 353 TCIAVIPVALRLPNRSHW---FHLALVVLVSACPCALILSTPVASFCALTKAATSGLLIK 412
             +   P      N +H+      ++ V+V ACPCAL L+TP A   A    AT+G+LIK
Sbjct: 582 GAVGAYPDEWLPENGTHFVFSLMFSISVVVIACPCALGLATPTAVMVATGVGATNGVLIK 641

Query: 413 GGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQAL-DKDNISFDTLLYWVSSIESKSSHP 472
           GGD LE   K+K + FDKTGT+T+G+  VT  +   + D   F TL   V+S E+ S HP
Sbjct: 642 GGDALEKAHKVKYVIFDKTGTLTQGKATVTTTKVFSEMDRGEFLTL---VASAEASSEHP 701

Query: 473 MAAALVDHGRSLSIFPKPE-----------------NVDDFQNFPGEGVHGRIDGKDIYI 532
           +A A+V + R    F +                   +  DF   PG+G+   ++ K I +
Sbjct: 702 LAKAIVAYARHFHFFDESTEDGETNNKDLQNSGWLLDTSDFSALPGKGIQCLVNEKMILV 761

Query: 533 GNRKIATRANCTTVPD-----MNDEARDGRTVGYIFCETSAVGTFSLSDSCRTGAKEAMD 592
           GNRK+ +  N   +PD     + D    G+T   +      VG   ++D  +  A   ++
Sbjct: 762 GNRKLMSE-NAINIPDHVEKFVEDLEESGKTGVIVAYNGKLVGVMGIADPLKREAALVVE 821

Query: 593 ELRSLGIKTAMLTGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREGPT-AMI 652
            L  +G++  M+TGD+   A    KE+G  +E V AE++P  K  +I   +++G T AM+
Sbjct: 822 GLLRMGVRPIMVTGDNWRTARAVAKEVG--IEDVRAEVMPAGKADVIRSLQKDGSTVAMV 881

Query: 653 GDGLNDAPALATADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIEN 712
           GDG+ND+PALA AD+G+++G +G+ +AIE  D +LM N++  +  AI L+R+   ++  N
Sbjct: 882 GDGINDSPALAAADVGMAIG-AGTDVAIEAADYVLMRNNLEDVITAIDLSRKTLTRIRLN 941

BLAST of Lag0033868 vs. TAIR 10
Match: AT1G63440.1 (heavy metal atpase 5 )

HSP 1 Score: 246.1 bits (627), Expect = 1.5e-64
Identity = 181/580 (31.21%), Postives = 296/580 (51.03%), Query Frame = 0

Query: 172 DYLEAATIVFLFTI-AEWLESRASHKANAVMSSLLSIAPQKAVLAD---TGEVVGADE-- 231
           D+ E + ++  F I  ++LE  A  K +  ++ L+++AP  A+L      G V G +E  
Sbjct: 404 DFFETSAMLISFIILGKYLEVMAKGKTSQAIAKLMNLAPDTAILLSLDKEGNVTGEEEID 463

Query: 232 ---VKLGTLLAVKAGEDIPIDGIVVEGKCEVDEKTLTGESFPVPKQKNSTVWAGTINLNG 291
              ++   ++ +  G  +  DG V+ G+  V+E  +TGE+ PV K+K  TV  GT+N NG
Sbjct: 464 GRLIQKNDVIKIVPGAKVASDGYVIWGQSHVNESMITGEARPVAKRKGDTVIGGTLNENG 523

Query: 292 YVTVKTTALAEDCVVAKMAKLVEEAQNNKSRTQRFIDICAKFYTPAVIIISTCIAVIPVA 351
            + VK T +  +  +A++ +LVE AQ  K+  Q+  D  +KF+ P VI +S       +A
Sbjct: 524 VLHVKVTRVGSESALAQIVRLVESAQLAKAPVQKLADRISKFFVPLVIFLSFSTW---LA 583

Query: 352 LRLPNRSHW------------FHLAL----VVLVSACPCALILSTPVASFCALTKAATSG 411
             L  + HW            F LAL     V+V ACPCAL L+TP A        A+ G
Sbjct: 584 WFLAGKLHWYPESWIPSSMDSFELALQFGISVMVIACPCALGLATPTAVMVGTGVGASQG 643

Query: 412 LLIKGGDFLETLAKIKIMAFDKTGTITRGEFMVTEFQALDKDNISFDTLLYWVSSIESKS 471
           +LIKGG  LE   K+  + FDKTGT+T G+ +V + + L   N+        V++ E  S
Sbjct: 644 VLIKGGQALERAHKVNCIVFDKTGTLTMGKPVVVKTKLL--KNMVLREFYELVAATEVNS 703

Query: 472 SHPMAAALVDHGRSL---SIFPKPENVDDFQNFPGEGVHGRIDGKDIYIGNRKIATRANC 531
            HP+A A+V++ +        P      DF +  G+GV   + G++I +GN+ +      
Sbjct: 704 EHPLAKAIVEYAKKFRDDEENPAWPEACDFVSITGKGVKATVKGREIMVGNKNLMNDHKV 763

Query: 532 TTVPDMNDEARDGRTVG----YIFCETSAVGTFSLSDSCRTGAKEAMDELRSLGIKTAML 591
               D  +   D   +      +   +  +G  S+SD  +  A+EA+  L+S+ IK+ M+
Sbjct: 764 IIPDDAEELLADSEDMAQTGILVSINSELIGVLSVSDPLKPSAREAISILKSMNIKSIMV 823

Query: 592 TGDSSAAALQAQKELGKALEIVHAELLPEDKTRLINDFKREG-PTAMIGDGLNDAPALAT 651
           TGD+   A    +E+G  ++ V AE  PE K   + + +  G   AM+GDG+ND+PAL  
Sbjct: 824 TGDNWGTANSIAREVG--IDSVIAEAKPEQKAEKVKELQAAGHVVAMVGDGINDSPALVA 883

Query: 652 ADIGISMGISGSALAIETGDVILMTNDIRKIPKAIKLARRANRKVIENVI------LSVV 710
           AD+G+++G +G+ +AIE  D++LM +++  +  AI L+R+   ++  N +      L  +
Sbjct: 884 ADVGMAIG-AGTDIAIEAADIVLMKSNLEDVITAIDLSRKTFSRIRLNYVWALGYNLMGI 943