Lag0033785 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0033785
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	E3 ubiquitin-protein ligase XBAT32
Location	chr3: 1823257 .. 1824748 (-)
RNA-Seq Expression	Lag0033785
Synteny	Lag0033785

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGAAACATATGGTGGAGGTTTATTCTCTGAAATGTCCATTCGAGAATTCCTTTGGGTTTTGTCATTTTGCTTGTCCTTTACATGTTTGCAGATAGTTTATCTTTTGCTTCAGTCAGGAGTTGACATTAATTTAAGGAAGTACAGGGGTCAGGTATTACAATACTTTCAAATTCTTTGCCCTTTTCCTTTCAGGGATATGCACATGCTGCGCACACATGTATTTCCTTCTCTTTTCACCACTTCATTTGCTCCATCGTGTGATTATCTATGCTATTAATCACCCGTTAAGTTGTAATTCTTCCGTTTCAAGATTAAATTCATAATGTATCTTGAGTTCATCATTGTGTTCCTTGCTTTGGTCAACAGACTGCTTTGATGCAAGCTTGTCAACATGGTCACTGGGAGGTTGTTTTGATTTTAGTACTTTTTGGAGCCAACGTGAGTAGTTTAGCTTTCATGTTATCTCTCTGGTTTGTGGCGCGAGCAACTTATTATTAGTCATTCATAAATTTGTTGCACATTTTAGGTCCATGAAGCAGATTTTTTGAATGGAGGGACTGCACTTCATCTTGCTGCTTCAAATGGTCATTCCCGGTGCATCAGGCTTCTTCTTGCTGACTATATTCCAAGCATACCAAAGTTCAGGGATAATGAGTAAAATAACAGACAATGAAGAATCAACCTCAGAGTTTGATCTTAGGTACATGTTGAATTCCTAATTGATGACCAAAAGTTTTCCCAGGTTGAAATGCTTATGATGGTTGATTGACAGCATAATTTTGTGTTTTAGGGCCTTGTCTCAGATTATTAACCAACCGGCAGATGGAGGCATTACCGACCGCTCTACACATGGCAGCCCTCAATGGGCAAGTTGAAAGCGTGCAGTTACTTCTGGAATTTGGGGCATCTGTTTCTGAGGTGACTGTGGGCGATGGAACCGTAATTGATCTAATAGGTTTCTCCCTTTGAATCTCTGCCTTCTCTTGCACTAAATCAACTTGAGGCCTTCTTTTTCATGTTAGCCTCTATTTTCCTTCCTAATCCACCATTTAACATGTCTGTCACTCTTTCTTTTGACAATAGCAAAACATGATTCTAAAATAAAGTTGTGGCGTTATATTTTAGATATGTTAAATATGGTACCTTATCCTTCTATTTTGTGGGCAAAGTGATAAGAACCGAAAGAAGGGATTGTTGAACAGTTTGATTTCTTATCTCTTATGATGATATAAATAGTTGTCTGTTGCAACTCTGGTAGGTGCTGGGAGCACAGCTCTTCATTATGTTGCATGCGGTGGTAATGCGAAATGTTGTCAAGTATGTGCTTCATTTAAAGGAAAATAATCTGAAATGGGTTCAATGAATGCCTTATTTTTTTCCTCTTTGAGGCTATTACTTATAGTTTTCTTTTCGTATAGCTTTTGATTGCTAGAGGTGCCAGTCTGACCATCGAAAATGAAAATGGGTACGTACTCTACTCATATTAG

mRNA sequence

ATGGAGGAAACATATGGTGGAGGTTTATTCTCTGAAATGTCCATTCGAGAATTCCTTTGGGTTTTGTCATTTTGCTTGTCCTTTACATGTTTGCAGATAGTTTATCTTTTGCTTCAGTCAGGAGTTGACATTAATTTAAGGAAGTACAGGGGTCAGACTGCTTTGATGCAAGCTTGTCAACATGGTCACTGGGAGGTTGTTTTGATTTTAGTACTTTTTGGAGCCAACGTCCATGAAGCAGATTTTTTGAATGGAGGGACTGCACTTCATCTTGCTGCTTCAAATGGTCATTCCCGGTGCATCAGGCTTCTTCTTGCTGACTATATTCCAAGCATACCAAAGTTCAGGGATAATGAATTATTAACCAACCGGCAGATGGAGGCATTACCGACCGCTCTACACATGGCAGCCCTCAATGGGCAAGTTGAAAGCGTGCAGTTACTTCTGGAATTTGGGGCATCTGTTTCTGAGGTGACTGTGGGCGATGGAACCGTAATTGATCTAATAGGTGCTGGGAGCACAGCTCTTCATTATGTTGCATGCGGTGGTAATGCGAAATGTTGTCAACTTTTGATTGCTAGAGGTGCCAGTCTGACCATCGAAAATGAAAATGGGTACGTACTCTACTCATATTAG

Coding sequence (CDS)

ATGGAGGAAACATATGGTGGAGGTTTATTCTCTGAAATGTCCATTCGAGAATTCCTTTGGGTTTTGTCATTTTGCTTGTCCTTTACATGTTTGCAGATAGTTTATCTTTTGCTTCAGTCAGGAGTTGACATTAATTTAAGGAAGTACAGGGGTCAGACTGCTTTGATGCAAGCTTGTCAACATGGTCACTGGGAGGTTGTTTTGATTTTAGTACTTTTTGGAGCCAACGTCCATGAAGCAGATTTTTTGAATGGAGGGACTGCACTTCATCTTGCTGCTTCAAATGGTCATTCCCGGTGCATCAGGCTTCTTCTTGCTGACTATATTCCAAGCATACCAAAGTTCAGGGATAATGAATTATTAACCAACCGGCAGATGGAGGCATTACCGACCGCTCTACACATGGCAGCCCTCAATGGGCAAGTTGAAAGCGTGCAGTTACTTCTGGAATTTGGGGCATCTGTTTCTGAGGTGACTGTGGGCGATGGAACCGTAATTGATCTAATAGGTGCTGGGAGCACAGCTCTTCATTATGTTGCATGCGGTGGTAATGCGAAATGTTGTCAACTTTTGATTGCTAGAGGTGCCAGTCTGACCATCGAAAATGAAAATGGGTACGTACTCTACTCATATTAG

Protein sequence

MEETYGGGLFSEMSIREFLWVLSFCLSFTCLQIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHLAASNGHSRCIRLLLADYIPSIPKFRDNELLTNRQMEALPTALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLLIARGASLTIENENGYVLYSY

Homology

[Close]

BLAST of Lag0033785 vs. NCBI nr
Match: XP_004149881.1 (E3 ubiquitin-protein ligase XBAT32 [Cucumis sativus] >KGN65279.1 hypothetical protein Csa_019889 [Cucumis sativus])

HSP 1 Score: 293.9 bits (751), Expect = 1.1e-75
Identity = 154/195 (78.97%), Postives = 163/195 (83.59%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IVYLLL SGVDINLR YRGQTALMQACQHGHWEVVLILVLFGANVH+AD+LNG TALHL
Sbjct: 65  EIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKFR--------DNE------------LLTNRQMEALPT 151
           AA+NGHSRCIRLLLADYIPSIP FR        DNE             + N++ +   T
Sbjct: 125 AATNGHSRCIRLLLADYIPSIPNFREIMSRQLTDNEESISEFDHRALSQIINQKADGGIT 184

Query: 152 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLL 207
           ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHY ACGGNAKCCQLL
Sbjct: 185 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQLL 244

[Close]

BLAST of Lag0033785 vs. NCBI nr
Match: XP_038879942.1 (probable E3 ubiquitin-protein ligase XBOS32 isoform X1 [Benincasa hispida])

HSP 1 Score: 293.1 bits (749), Expect = 1.9e-75
Identity = 151/194 (77.84%), Postives = 163/194 (84.02%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IVYLLLQSGVDIN+R YRGQTALMQACQHGHWEVVLILVLFGANVH+AD+LNGGTALHL
Sbjct: 65  EIVYLLLQSGVDINIRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGGTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKFR-------DNE------------LLTNRQMEALPTA 151
           AA+NGHSRCIRLLLADYIPS+P FR       DNE             + N+  +   TA
Sbjct: 125 AATNGHSRCIRLLLADYIPSMPNFREIMSQLTDNEESILEFDHRALSKIINQTADGGITA 184

Query: 152 LHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLLI 207
           LHMAALNGQVESVQLLLEFGASVSEVT+GDGT+IDLIGAGSTALHY ACGGNAKCCQLLI
Sbjct: 185 LHMAALNGQVESVQLLLEFGASVSEVTLGDGTIIDLIGAGSTALHYAACGGNAKCCQLLI 244

[Close]

BLAST of Lag0033785 vs. NCBI nr
Match: XP_008443799.1 (PREDICTED: E3 ubiquitin-protein ligase XBAT32 [Cucumis melo] >KAA0035132.1 E3 ubiquitin-protein ligase XBAT32 [Cucumis melo var. makuwa])

HSP 1 Score: 292.0 bits (746), Expect = 4.2e-75
Identity = 153/195 (78.46%), Postives = 163/195 (83.59%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IVYLLL SGVDINLR YRGQTALMQACQHGHWEVVLILVLFGANVH+AD+LNG TALHL
Sbjct: 65  EIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKFR--------DNE------------LLTNRQMEALPT 151
           AA+NGHSRCIRLLLADYIPSIP FR        D+E             + N++ +   T
Sbjct: 125 AATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKADGGIT 184

Query: 152 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLL 207
           ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHY ACGGNAKCCQLL
Sbjct: 185 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQLL 244

[Close]

BLAST of Lag0033785 vs. NCBI nr
Match: TYJ95947.1 (E3 ubiquitin-protein ligase XBAT32 [Cucumis melo var. makuwa])

HSP 1 Score: 292.0 bits (746), Expect = 4.2e-75
Identity = 153/195 (78.46%), Postives = 163/195 (83.59%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IVYLLL SGVDINLR YRGQTALMQACQHGHWEVVLILVLFGANVH+AD+LNG TALHL
Sbjct: 65  EIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKFR--------DNE------------LLTNRQMEALPT 151
           AA+NGHSRCIRLLLADYIPSIP FR        D+E             + N++ +   T
Sbjct: 125 AATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKADGGIT 184

Query: 152 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLL 207
           ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHY ACGGNAKCCQLL
Sbjct: 185 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQLL 244

[Close]

BLAST of Lag0033785 vs. NCBI nr
Match: XP_022928988.1 (probable E3 ubiquitin-protein ligase XBOS32 [Cucurbita moschata])

HSP 1 Score: 290.4 bits (742), Expect = 1.2e-74
Identity = 153/194 (78.87%), Postives = 161/194 (82.99%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IV LLLQSGVDINLR YRGQTALMQACQ GHWEVVLILVLFGANVH+AD+LNGGTALHL
Sbjct: 65  EIVELLLQSGVDINLRNYRGQTALMQACQQGHWEVVLILVLFGANVHKADYLNGGTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKFR--------DNELLTNRQMEALP-----------TA 151
           AASNGHSRCIRLLLADYIPS+P FR        D E ++     AL            TA
Sbjct: 125 AASNGHSRCIRLLLADYIPSMPNFRDIMSKVTGDEESISEFDHRALSQIINQPADGGITA 184

Query: 152 LHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLLI 207
           LHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHY ACGGNAKCCQ+LI
Sbjct: 185 LHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQILI 244

[Close]

BLAST of Lag0033785 vs. ExPASy Swiss-Prot
Match: Q6NLQ8 (E3 ubiquitin-protein ligase XBAT32 OS=Arabidopsis thaliana OX=3702 GN=XBAT32 PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 3.5e-64
Identity = 123/191 (64.40%), Postives = 148/191 (77.49%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IV LL++SGVDINLR YRGQTALMQACQHGHWEVVLIL+LFGAN+H +D+LNGGTALHL
Sbjct: 65  EIVSLLVESGVDINLRNYRGQTALMQACQHGHWEVVLILILFGANIHRSDYLNGGTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPK--------------FRDNEL--LTNRQMEALPTALHM 151
           AA NGH RCIR+LL++YIPS+P               F  + L  + NR  +   T LH+
Sbjct: 125 AALNGHPRCIRILLSEYIPSVPNCWSLLKNKKTSVAGFDSSVLHEVINRAADGGITPLHV 184

Query: 152 AALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLLIARG 207
           AALNG +E+VQLLL+ GASV++VTV DGT IDLIGAGSTALHY +CGGN +CCQLLI++G
Sbjct: 185 AALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCCQLLISKG 244

[Close]

BLAST of Lag0033785 vs. ExPASy Swiss-Prot
Match: Q6KAE5 (Probable E3 ubiquitin-protein ligase XBOS32 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS32 PE=2 SV=2)

HSP 1 Score: 235.7 bits (600), Expect = 4.7e-61
Identity = 119/194 (61.34%), Postives = 144/194 (74.23%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IV LLL+SGV+INLR YRGQTALMQACQ+GHWEVV  L+LF ANVH  D+LNGG+ALH 
Sbjct: 65  EIVSLLLESGVEINLRNYRGQTALMQACQYGHWEVVQTLMLFNANVHRTDYLNGGSALHF 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKFRDNE-------------------LLTNRQMEALPTA 151
           AA +GH+RC+RL+LADY+PS+P F ++                     + N++ +   T 
Sbjct: 125 AALHGHARCLRLVLADYVPSMPNFWNSMKDSLSEEGPSADLDEDGLFKMVNQKADGGLTP 184

Query: 152 LHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLLI 207
           LHMAALNG VE VQLLL+ GASV E T+ DGT IDLIGAGST LHY ACGGNA CCQLLI
Sbjct: 185 LHMAALNGHVECVQLLLDLGASVIEATIEDGTTIDLIGAGSTPLHYAACGGNAVCCQLLI 244

[Close]

BLAST of Lag0033785 vs. ExPASy Swiss-Prot
Match: Q7EZ44 (Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS35 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 6.6e-55
Identity = 109/193 (56.48%), Postives = 138/193 (71.50%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IV LL++SGVDINLR  RGQTALMQAC +GHW+VV ILVLF AN+H+ D  +G TA+H 
Sbjct: 65  EIVSLLIESGVDINLRNCRGQTALMQACLYGHWKVVQILVLFKANIHKKDCFSGATAIHF 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKF----------RDNE---------LLTNRQMEALPTA 151
           AA  GH+RC+RLL+ADY+PS+P+F            N+          L N + +   T 
Sbjct: 125 AALKGHTRCLRLLVADYVPSLPEFWSVMHAKCTDETNKEAFDAVALRRLINNKSDGGVTP 184

Query: 152 LHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLLI 206
           LH+AAL+G  E VQLLL+ GASVSEVT+ DG+ IDLIG+GST LHY ACGG+A CCQLL+
Sbjct: 185 LHLAALHGHAECVQLLLDLGASVSEVTINDGSTIDLIGSGSTPLHYAACGGSAVCCQLLV 244

[Close]

BLAST of Lag0033785 vs. ExPASy Swiss-Prot
Match: Q4FE45 (E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.0e-48
Identity = 106/192 (55.21%), Postives = 131/192 (68.23%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IV LLL++G D+N R Y GQTALMQAC++GHWEVV  L+LF  NV  AD+L G TALH 
Sbjct: 59  EIVGLLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHF 118

Query: 92  AASNGHSRCIRLLLADYIP-----SIP---------KFRDNEL--LTNRQMEALPTALHM 151
           AA NGH+RCIRL+LAD++P     S+P         K   + L    N+  +   TALHM
Sbjct: 119 AAVNGHARCIRLVLADFLPSDKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALHM 178

Query: 152 AALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLLIARG 208
           AALNG  + VQLLL+  A+VS VT   GT +D+IGAGST LHY ACGGN KCCQ+L+ARG
Sbjct: 179 AALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARG 238

[Close]

BLAST of Lag0033785 vs. ExPASy Swiss-Prot
Match: Q337A0 (Probable E3 ubiquitin-protein ligase XBOS33 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS33 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 5.0e-47
Identity = 106/216 (49.07%), Postives = 135/216 (62.50%), Query Frame = 0


Query: 22  LSFCLSFTCLQIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEAD 81
           L F  +   L IV LLL+ G D+N+R Y GQTALM AC+HGHWEVV +L+LF  NV  AD
Sbjct: 49  LHFAAAKGHLDIVTLLLEKGADVNVRNYCGQTALMHACRHGHWEVVQMLLLFRCNVTRAD 108

Query: 82  FLNGGTALHLAASNGHSRCIRLLLADYIPSIP---------------------------- 141
           +L+G TALH AA +G  RC+RLLLAD++PS P                            
Sbjct: 109 YLSGRTALHFAAHDGLVRCVRLLLADFVPSAPLEDGASSTVDGGECQTNSGSSPCSSLGL 168

Query: 142 KFRDNELL--TNRQMEALPTALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGA 201
           KF ++  L   N+  +   TALHMAALNG  + +QLL++ GA+VS VT   GT  +LIGA
Sbjct: 169 KFNESARLRYINKPADGGVTALHMAALNGHFDCMQLLIDLGANVSAVTFPYGTTANLIGA 228

Query: 202 GSTALHYVACGGNAKCCQLLIARGASLTIENENGYV 208
           GST LHY A GGNA+CCQLL+++GAS    N NG++
Sbjct: 229 GSTPLHYAAGGGNAECCQLLLSKGASKLTLNCNGWL 264

[Close]

BLAST of Lag0033785 vs. ExPASy TrEMBL
Match: A0A0A0LWB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G294100 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 5.4e-76
Identity = 154/195 (78.97%), Postives = 163/195 (83.59%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IVYLLL SGVDINLR YRGQTALMQACQHGHWEVVLILVLFGANVH+AD+LNG TALHL
Sbjct: 65  EIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKFR--------DNE------------LLTNRQMEALPT 151
           AA+NGHSRCIRLLLADYIPSIP FR        DNE             + N++ +   T
Sbjct: 125 AATNGHSRCIRLLLADYIPSIPNFREIMSRQLTDNEESISEFDHRALSQIINQKADGGIT 184

Query: 152 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLL 207
           ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHY ACGGNAKCCQLL
Sbjct: 185 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQLL 244

[Close]

BLAST of Lag0033785 vs. ExPASy TrEMBL
Match: A0A5A7SV55 (E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G002150 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 2.0e-75
Identity = 153/195 (78.46%), Postives = 163/195 (83.59%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IVYLLL SGVDINLR YRGQTALMQACQHGHWEVVLILVLFGANVH+AD+LNG TALHL
Sbjct: 65  EIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKFR--------DNE------------LLTNRQMEALPT 151
           AA+NGHSRCIRLLLADYIPSIP FR        D+E             + N++ +   T
Sbjct: 125 AATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKADGGIT 184

Query: 152 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLL 207
           ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHY ACGGNAKCCQLL
Sbjct: 185 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQLL 244

[Close]

BLAST of Lag0033785 vs. ExPASy TrEMBL
Match: A0A5D3B9Z5 (E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold271G00090 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 2.0e-75
Identity = 153/195 (78.46%), Postives = 163/195 (83.59%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IVYLLL SGVDINLR YRGQTALMQACQHGHWEVVLILVLFGANVH+AD+LNG TALHL
Sbjct: 65  EIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKFR--------DNE------------LLTNRQMEALPT 151
           AA+NGHSRCIRLLLADYIPSIP FR        D+E             + N++ +   T
Sbjct: 125 AATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKADGGIT 184

Query: 152 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLL 207
           ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHY ACGGNAKCCQLL
Sbjct: 185 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQLL 244

[Close]

BLAST of Lag0033785 vs. ExPASy TrEMBL
Match: A0A1S3B8E1 (E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo OX=3656 GN=LOC103487302 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 2.0e-75
Identity = 153/195 (78.46%), Postives = 163/195 (83.59%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IVYLLL SGVDINLR YRGQTALMQACQHGHWEVVLILVLFGANVH+AD+LNG TALHL
Sbjct: 65  EIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKFR--------DNE------------LLTNRQMEALPT 151
           AA+NGHSRCIRLLLADYIPSIP FR        D+E             + N++ +   T
Sbjct: 125 AATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKADGGIT 184

Query: 152 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLL 207
           ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHY ACGGNAKCCQLL
Sbjct: 185 ALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQLL 244

[Close]

BLAST of Lag0033785 vs. ExPASy TrEMBL
Match: A0A6J1EQQ1 (probable E3 ubiquitin-protein ligase XBOS32 OS=Cucurbita moschata OX=3662 GN=LOC111435719 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 5.9e-75
Identity = 153/194 (78.87%), Postives = 161/194 (82.99%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IV LLLQSGVDINLR YRGQTALMQACQ GHWEVVLILVLFGANVH+AD+LNGGTALHL
Sbjct: 65  EIVELLLQSGVDINLRNYRGQTALMQACQQGHWEVVLILVLFGANVHKADYLNGGTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPKFR--------DNELLTNRQMEALP-----------TA 151
           AASNGHSRCIRLLLADYIPS+P FR        D E ++     AL            TA
Sbjct: 125 AASNGHSRCIRLLLADYIPSMPNFRDIMSKVTGDEESISEFDHRALSQIINQPADGGITA 184

Query: 152 LHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLLI 207
           LHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHY ACGGNAKCCQ+LI
Sbjct: 185 LHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQILI 244

[Close]

BLAST of Lag0033785 vs. TAIR 10
Match: AT5G57740.1 (XB3 ortholog 2 in Arabidopsis thaliana )

HSP 1 Score: 246.1 bits (627), Expect = 2.5e-65
Identity = 123/191 (64.40%), Postives = 148/191 (77.49%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IV LL++SGVDINLR YRGQTALMQACQHGHWEVVLIL+LFGAN+H +D+LNGGTALHL
Sbjct: 65  EIVSLLVESGVDINLRNYRGQTALMQACQHGHWEVVLILILFGANIHRSDYLNGGTALHL 124

Query: 92  AASNGHSRCIRLLLADYIPSIPK--------------FRDNEL--LTNRQMEALPTALHM 151
           AA NGH RCIR+LL++YIPS+P               F  + L  + NR  +   T LH+
Sbjct: 125 AALNGHPRCIRILLSEYIPSVPNCWSLLKNKKTSVAGFDSSVLHEVINRAADGGITPLHV 184

Query: 152 AALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLLIARG 207
           AALNG +E+VQLLL+ GASV++VTV DGT IDLIGAGSTALHY +CGGN +CCQLLI++G
Sbjct: 185 AALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCCQLLISKG 244

[Close]

BLAST of Lag0033785 vs. TAIR 10
Match: AT5G07270.1 (XB3 ortholog 3 in Arabidopsis thaliana )

HSP 1 Score: 193.7 bits (491), Expect = 1.5e-49
Identity = 106/192 (55.21%), Postives = 131/192 (68.23%), Query Frame = 0


Query: 32  QIVYLLLQSGVDINLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHL 91
           +IV LLL++G D+N R Y GQTALMQAC++GHWEVV  L+LF  NV  AD+L G TALH 
Sbjct: 59  EIVGLLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHF 118

Query: 92  AASNGHSRCIRLLLADYIP-----SIP---------KFRDNEL--LTNRQMEALPTALHM 151
           AA NGH+RCIRL+LAD++P     S+P         K   + L    N+  +   TALHM
Sbjct: 119 AAVNGHARCIRLVLADFLPSDKLNSLPETGVVTAKNKSEQSALSKFVNKAADGGITALHM 178

Query: 152 AALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLLIARG 208
           AALNG  + VQLLL+  A+VS VT   GT +D+IGAGST LHY ACGGN KCCQ+L+ARG
Sbjct: 179 AALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARG 238

[Close]

BLAST of Lag0033785 vs. TAIR 10
Match: AT1G07710.1 (Ankyrin repeat family protein )

HSP 1 Score: 61.2 bits (147), Expect = 1.1e-09
Identity = 50/147 (34.01%), Postives = 70/147 (47.62%), Query Frame = 0


Query: 53  TALMQACQHGHWEVVLILVLFGANVHEADFLNGGTALHLAASNGHSRCIRLLLADYIPSI 112
           TAL  A   GH EVV  L+  G+++      NG TALH A+ NGH + I+ LLA      
Sbjct: 137 TALHTAATQGHTEVVNFLLELGSSLAGIAKSNGKTALHSASRNGHVKVIKALLA------ 196

Query: 113 PKFRDNELLTNRQMEALPTALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAG 172
                   +  R  +   TALHMA     VE V+ L++  A  S + + D         G
Sbjct: 197 ----SEPAIAIRMDKKGQTALHMAVKGTNVEVVEELIK--ADRSSINIAD-------TKG 256

Query: 173 STALHYVACGGNAKCCQLLIARGASLT 200
           +TALH  A  G ++  +LL+A   + T
Sbjct: 257 NTALHIAARKGRSQIVKLLLANNMTDT 264

[Close]

BLAST of Lag0033785 vs. TAIR 10
Match: AT2G03430.1 (Ankyrin repeat family protein )

HSP 1 Score: 55.8 bits (133), Expect = 4.7e-08
Identity = 49/169 (28.99%), Postives = 74/169 (43.79%), Query Frame = 0


Query: 39  QSGVDINLRKYRGQTALMQACQHGHWEVVLILVLF--GANVHEADFLNGGTALHLAASNG 98
           Q    +N R   G++ L  A   GH ++V +L        V  +    G   LH AAS G
Sbjct: 35  QLSKSLNFRNEDGRSLLHVAASFGHSQIVKLLSSSDEAKTVINSKDDEGWAPLHSAASIG 94

Query: 99  HSRCIRLLLADYIPSIPKFRDNELLTNRQMEALPTALHMAALNGQVESVQLLLEFGASVS 158
           ++  + +LL          R  ++  N +     TALH AA  G++E  QLLL  GA ++
Sbjct: 95  NAELVEVLLT---------RGADV--NAKNNGGRTALHYAASKGRLEIAQLLLTHGAKIN 154

Query: 159 EVTVGDGTVIDLIGAGSTALHYVACGGNAKCCQLLIARGASLTIENENG 206
                   + D +G   T LH  A  G  + C+ LI  GA +   ++ G
Sbjct: 155 --------ITDKVGC--TPLHRAASVGKLEVCEFLIEEGAEIDATDKMG 182

[Close]

BLAST of Lag0033785 vs. TAIR 10
Match: AT2G31820.1 (Ankyrin repeat family protein )

HSP 1 Score: 52.4 bits (124), Expect = 5.2e-07
Identity = 42/157 (26.75%), Postives = 75/157 (47.77%), Query Frame = 0


Query: 44  INLRKYRGQTALMQACQHGHWEVVLILVLFGANVHEADFL--NGGTALHLAASNGHSRCI 103
           ++ +   G+T L  A ++GH  +V+  +L   ++  A     NG    H+AA  GH   +
Sbjct: 182 LSKQNLEGETPLYTAAENGH-SIVVEEMLKHMDLETASIAARNGFDPFHVAAKQGHLEVL 241

Query: 104 RLLLADYIPSIPKFRDNELLTNRQMEALPTALHMAALNGQVESVQLLLEFGASVSEVTVG 163
           ++LL  + P++    D          +  TALH AA  G ++ V LLLE  ++++++   
Sbjct: 242 KILLETF-PNLAMTTD---------LSCTTALHTAATQGHIDVVNLLLETDSNLAKIAKN 301

Query: 164 DGTVIDLIGAGSTALHYVACGGNAKCCQLLIARGASL 199
           +         G TALH  A  G+ +  + LI +  S+
Sbjct: 302 N---------GKTALHSAARMGHVEVVKSLIGKDPSI 318

The following BLAST results are available for this feature:

BLAST of Lag0033785 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149881.1	1.1e-75	78.97	E3 ubiquitin-protein ligase XBAT32 [Cucumis sativus] >KGN65279.1 hypothetical pr...	[more]
XP_038879942.1	1.9e-75	77.84	probable E3 ubiquitin-protein ligase XBOS32 isoform X1 [Benincasa hispida]	[more]
XP_008443799.1	4.2e-75	78.46	PREDICTED: E3 ubiquitin-protein ligase XBAT32 [Cucumis melo] >KAA0035132.1 E3 ub...	[more]
TYJ95947.1	4.2e-75	78.46	E3 ubiquitin-protein ligase XBAT32 [Cucumis melo var. makuwa]	[more]
XP_022928988.1	1.2e-74	78.87	probable E3 ubiquitin-protein ligase XBOS32 [Cucurbita moschata]	[more]

BLAST of Lag0033785 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NLQ8	3.5e-64	64.40	E3 ubiquitin-protein ligase XBAT32 OS=Arabidopsis thaliana OX=3702 GN=XBAT32 PE=...	[more]
Q6KAE5	4.7e-61	61.34	Probable E3 ubiquitin-protein ligase XBOS32 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q7EZ44	6.6e-55	56.48	Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q4FE45	2.0e-48	55.21	E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=...	[more]
Q337A0	5.0e-47	49.07	Probable E3 ubiquitin-protein ligase XBOS33 OS=Oryza sativa subsp. japonica OX=3...	[more]

BLAST of Lag0033785 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LWB1	5.4e-76	78.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G294100 PE=4 SV=1	[more]
A0A5A7SV55	2.0e-75	78.46	E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A5D3B9Z5	2.0e-75	78.46	E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B8E1	2.0e-75	78.46	E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo OX=3656 GN=LOC103487302 PE=4 ...	[more]
A0A6J1EQQ1	5.9e-75	78.87	probable E3 ubiquitin-protein ligase XBOS32 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of Lag0033785 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G57740.1	2.5e-65	64.40	XB3 ortholog 2 in Arabidopsis thaliana	[more]
AT5G07270.1	1.5e-49	55.21	XB3 ortholog 3 in Arabidopsis thaliana	[more]
AT1G07710.1	1.1e-09	34.01	Ankyrin repeat family protein	[more]
AT2G03430.1	4.7e-08	28.99	Ankyrin repeat family protein	[more]
AT2G31820.1	5.2e-07	26.75	Ankyrin repeat family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002110	Ankyrin repeat	PRINTS	PR01415	ANKYRIN	coord: 85..100 score: 44.07 coord: 144..158 score: 36.31
IPR002110	Ankyrin repeat	SMART	SM00248	ANK_2a	coord: 84..114 e-value: 0.059 score: 22.5 coord: 171..200 e-value: 0.0013 score: 28.0 coord: 50..79 e-value: 0.0044 score: 26.2 coord: 128..157 e-value: 0.013 score: 24.7 coord: 17..46 e-value: 1200.0 score: 4.4
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 50..82 score: 10.579329
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 131..160 score: 10.926559
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 171..203 score: 10.81972
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 84..105 score: 9.99171
IPR036770	Ankyrin repeat-containing domain superfamily	GENE3D	1.25.40.20		coord: 25..111 e-value: 5.5E-22 score: 79.8
IPR036770	Ankyrin repeat-containing domain superfamily	GENE3D	1.25.40.20		coord: 112..209 e-value: 8.0E-18 score: 66.6
IPR036770	Ankyrin repeat-containing domain superfamily	SUPERFAMILY	48403	Ankyrin repeat	coord: 22..209
IPR020683	Ankyrin repeat-containing domain	PFAM	PF12796	Ank_2	coord: 130..201 e-value: 1.8E-9 score: 38.0 coord: 28..107 e-value: 1.4E-14 score: 54.5
None	No IPR available	PANTHER	PTHR24128:SF63	E3 UBIQUITIN-PROTEIN LIGASE XBOS32 ISOFORM X1-RELATED	coord: 121..207
None	No IPR available	PANTHER	PTHR24128:SF63	E3 UBIQUITIN-PROTEIN LIGASE XBOS32 ISOFORM X1-RELATED	coord: 24..116
None	No IPR available	PANTHER	PTHR24128	HOMEOBOX PROTEIN WARIAI	coord: 121..207
None	No IPR available	PANTHER	PTHR24128	HOMEOBOX PROTEIN WARIAI	coord: 24..116
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 171..203 score: 9.462976
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 50..81 score: 9.728291
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 84..105 score: 9.967073
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 131..156 score: 10.418106

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0033785.1	Lag0033785.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0140096	catalytic activity, acting on a protein
molecular_function	GO:0005515	protein binding
molecular_function	GO:0016740	transferase activity