Lag0033759 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033759
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAUGMIN subunit 8-like
Locationchr3: 1622293 .. 1626246 (-)
RNA-Seq ExpressionLag0033759
SyntenyLag0033759
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTATGCGAATCGGCACGGGCATTTAGAAAGCATACAGTAGCAGCGACGCTAAGACAACCGCTGGTTCCTGCAGAAAAGAACAATGAAGTTATCACACGGTCTTCCTCGCGTTCCAAGAACAAGTCACCTTCTCCTTCATCGTTGTCTGGTTCTCGGCGTTGCCCATCACCAAGCATCACGCGGACGGTTTCTACACCCTCCCAATTGGTTCTTAAAAGAGCTCAATCAGCAGAGAGGAAGCGACCCTCCACACCCCCTTCTCCTTCAAGCCCGGCGACACCGATTCATGGTTCACCAGCAGATGTACAGTTGCTATCGAAAAGAATGATTGGTGGTCGACCTGAGGGTTTATGGCCCTCTACGATGAGAAGTTTGAGCGTCTCCTTCCAATCTGATACAATTTCTATTCCTGTTAGTAAGAAGGAAAAACCCGTGTTATCTTCTCCTTCTGATCGAACTCTGAGGCCTTCTTCCAACTTTCCTTCTAAGCAGGCTGAAACCCAAACTGTTGCACGGAAGCCCACACCAGAAAGAAAGAAGAGTCCTCTTAGAAGTAAAAATGGGCATGACCAATCAGAAAATTTCAAGCCAGTTGATGGGTCACGTACCCAATTCGTAGATCAGCACAGATGGCCAAGTAGAGTTGGTGCGAAAGCATCCTCCAATTCATTGACTTGTAGTGTGGATCTCACTGATAAAAGAATCCCAAGCTTAAATAAGCCCCTCAGAGGAAACGAATTATCTTCTACGAGGGCAACAACGGTCGAGACTATTAACAAATCTTTACAGAGATCGACCAGTGGTGTTATGAGGCTAGCATATGTTGATGGAAGGAGTAGGGAAGAATTTGAGGCAAAATCAGCTGCTGACAATTCAGTGCAGGAGTCTGCAGCTAACAACGTTGTTTCTTCAAGTTTAGCAGGCATAAAAATAACCACAAACCGAGTTGTGAGATATGACTCACCTACTCTTGGCCCACGACCATCTTCACCTTCTAAGACACCAGTATTATCCTCTGTTACTAGAGGAGTTAGCCCATCTCGAATAAGACCATCAACTACACCTCCTCGAGGAATTAGCTCATCACGAATAAGACCTTCAAATTCAACTCAATCCAATGCTTCAACTTCTGTACTTAGCTTCATAGCAGATTTTAAGAAGGGAAAGAAGGCGGCAAGCTATATAGAAGGTGCTCATCAACTACGGCTTCTATACAATAGACATTTGCAGTGGAGATGTGCTAATGCACGGGCAGAGGCTGTTCTGCGCAATCAGGAGGTGAAAGCAGAGGTAGGTATCCATGTCAATCTTGCTTGATTATAGACCCTTTTTTTGGCGACAACTTGTTCATTTACTGTGTTGGCACCAGATGTGATTAATGTTACTTGCGATTGTGAACAGTCTGATATTTTGATGATTTAACCTTTGCAAAGATATTTTGTATAGTTGTTCACATATTTGCTTGTGCTCAGAAACTATGAGTTATATTCTGACAGTCCAACCTGGGGAAGATTTTATTCTGACTTGGCAGTAATTTTTAGGGACAGATTTTATTTTTTTTATCCAATTAGCAGCATTATTGCTTGTATACTTGGCAATTTAAAGATTAACAACCTTTGTGCTTTGGTGCAGAGAACTCTTCTTGGCGTGTGGAATACTACAGTAAATCTGTGGGATTCAGTAATCAGAAAAAGAATTAATCTGCAGCAGCTGAAGCTAGAGCTCAAATTCATCTCAATTATGAATGATCAAGTAATACGATTAATTCATTGAAAGCCATTGTATATCATTAGTGTACTTTATATAGCACGGTTCTTTTGTATTTTCATTGTAATCCAGAGTTTTGGGTATAAGTGGTGCTTAAATTATAACACTTAATAGATAACATCAATGCTGCCAATTATGGAATACTTATTATGGACTTGCCAACCATGGTAAGGATTTTGAATAAAAGTTGAGACTTATGCAATAGGTATTTTAGCACATGATCTGAACCTCAATGTTTCAGATTGCAATGTATAAAGTTTTAGCATAAATTGTCAACTTGGAGTAGCAGAGTATGGAACTTGCTTTGTTTCTCCAGCAATATAGAAGTACAACTAGAGTCGTATCACATGTTTTAACAGGGTTTTTTTAGGTGAATTATTTTATTTGAGAAACATATCCTTTTTTGGTTAGTCTGTTAAATCAGAACTTTTTCTAGTCCTTTAAGTTTGGAGTTATAATTTAATTCCCCTCCTGCTTGTACTTGAACAACCATTTTCTGTCATGTATGCTTCACCTAAATGATATGATTTTTTGTTACATGTGATTGATGCAGATGAGCTGCCTCAACGAATGGGCCGTGCTTGAAAGAAGTCATAATCGATCCTTATCAGGTGTCATAGATGATTTGGAGTCGAGCACTCTTCGTATTCCAGTAACTGGAGGAGCAAAAGTATGTTACATTGGCCAGTAATTTGTTTTAATAATGGGAAGCCAAATATTGGTAACTGACAGTAATTGCTTATTATTTACAGGCAGATGCTGGCTCTTTAAACGGTGCTATTTGTTCAGCCGTTGAGATCATGCAAGCAATGGGATCCTCTATTTGCTCCCTGCTCCCAAGGGTAGATTAATATAAAACTTTCTTGAATGATATCGATATTTATTAGCTTTCCTCCACATCTTCCTTGAAATCAAGAGCCTCTGTAATTGTTATGGACATTGACCAATCTTGTCATCTCATAATGCAATTGATGTTCATAGTGCTTGAGAATAAGAATAATTCTGATCATCATAACTAGAGGAGACAAACTTTTTTTTTTTTTTTTTGACGTTTGTAAACCGTCAAATTGCCTCTGATTTGGATCTCTCTGTAGTTGGCATTTTATCCCGTGAGATCTACATGTTGCGGAGTTCAAAATTCATTGTGTTTATCATTTGCTTGATAATTGTTATAAATATATACTAATAGCAAACTTCAAGTGATATACTTCAAGAAAGTTGGCTCAAGGAATCGATGACAGTTTATCATGCATCATAGTGAAAGGAACTTTAATTTGTTGTACTGTCTCATTTCGATGAAATATCAAATGAACCTTCATTTACTATTAAAGTATTGTTATTTGTGTGCTCGTGAAAGTTTCACTCCTCATTATATTGCCTGCCATATTCCTTTTTATATCTTTACAGTTTGCATCATGGAGTGACTTTGTCTTATAATTCTATGTCTTTTAGGCCATTGAAAATCTAATTTATTGAATTATATGCTGATGTTTGGATTCACAGTTATGTGACTTGTAACATTTGATTGGGAGCTTCAATGCTATCTTGTCTGCAGTTATAGTCCGTGTTATCTACTGTTCATCATGGTCTTATTTGTTTTTCAGGTGGAGGTAATACAGACGTTGGTTCTTGAACTTGCTATTGTCGCAGCCCAAGAAAAAGCAATGCTCGATGAATGTATAGCATTGTTGGCATCAACAGCGGCTTTGCAGGTGGGGATGATATTTCAATGTTGTATATAAATTGACAAATGGATTTACTTGTCTTGATCTTGATTTGGAACTTTTTTGTTGTCCCACAAATCTCATTGAAGTCAATAGAAAAAACTATTGCCCAAATTTTATGCCTTTTGTTCACTGAGTTTTGGTTCATTCTTCTTCATGAAGCTAAGCACTGTTGCTGATAAGAGCTGTTTATTTAGCGAGAGTAAAGTACATATTGCAATTATCAATCTATCAGTTTTTGCAATTGCTTTGAAAACAAGAATCCGTTTTTATCTGTGTGATAATAACCCTTCGCACATCAAGCACAGTTGAATTTATTGATTGCTTTACTCTACAAGAAAGCATATAAGTAACAGTATGCAATTTGAAAGCAGGCTCAGGAGCAGAGTTTGTGGACGCATCTCATACAAATGAAACAAGGACTGGAAACGGGCTGCCAGTTTTAGGCGGTGGTTAA

mRNA sequence

ATGGATGTATGCGAATCGGCACGGGCATTTAGAAAGCATACAGTAGCAGCGACGCTAAGACAACCGCTGGTTCCTGCAGAAAAGAACAATGAAGTTATCACACGGTCTTCCTCGCGTTCCAAGAACAAGTCACCTTCTCCTTCATCGTTGTCTGGTTCTCGGCGTTGCCCATCACCAAGCATCACGCGGACGGTTTCTACACCCTCCCAATTGGTTCTTAAAAGAGCTCAATCAGCAGAGAGGAAGCGACCCTCCACACCCCCTTCTCCTTCAAGCCCGGCGACACCGATTCATGGTTCACCAGCAGATGTACAGTTGCTATCGAAAAGAATGATTGGTGGTCGACCTGAGGGTTTATGGCCCTCTACGATGAGAAGTTTGAGCGTCTCCTTCCAATCTGATACAATTTCTATTCCTGTTAGTAAGAAGGAAAAACCCGTGTTATCTTCTCCTTCTGATCGAACTCTGAGGCCTTCTTCCAACTTTCCTTCTAAGCAGGCTGAAACCCAAACTGTTGCACGGAAGCCCACACCAGAAAGAAAGAAGAGTCCTCTTAGAAGTAAAAATGGGCATGACCAATCAGAAAATTTCAAGCCAGTTGATGGGTCACGTACCCAATTCGTAGATCAGCACAGATGGCCAAGTAGAGTTGGTGCGAAAGCATCCTCCAATTCATTGACTTGTAGTGTGGATCTCACTGATAAAAGAATCCCAAGCTTAAATAAGCCCCTCAGAGGAAACGAATTATCTTCTACGAGGGCAACAACGGTCGAGACTATTAACAAATCTTTACAGAGATCGACCAGTGGTGTTATGAGGCTAGCATATGTTGATGGAAGGAGTAGGGAAGAATTTGAGGCAAAATCAGCTGCTGACAATTCAGTGCAGGAGTCTGCAGCTAACAACGTTGTTTCTTCAAGTTTAGCAGGCATAAAAATAACCACAAACCGAGTTGTGAGATATGACTCACCTACTCTTGGCCCACGACCATCTTCACCTTCTAAGACACCAGTATTATCCTCTGTTACTAGAGGAGTTAGCCCATCTCGAATAAGACCATCAACTACACCTCCTCGAGGAATTAGCTCATCACGAATAAGACCTTCAAATTCAACTCAATCCAATGCTTCAACTTCTGTACTTAGCTTCATAGCAGATTTTAAGAAGGGAAAGAAGGCGGCAAGCTATATAGAAGGTGCTCATCAACTACGGCTTCTATACAATAGACATTTGCAGTGGAGATGTGCTAATGCACGGGCAGAGGCTGTTCTGCGCAATCAGGAGGTGAAAGCAGAGAGAACTCTTCTTGGCGTGTGGAATACTACAGTAAATCTGTGGGATTCAGTAATCAGAAAAAGAATTAATCTGCAGCAGCTGAAGCTAGAGCTCAAATTCATCTCAATTATGAATGATCAAATGAGCTGCCTCAACGAATGGGCCGTGCTTGAAAGAAGTCATAATCGATCCTTATCAGGTGTCATAGATGATTTGGAGTCGAGCACTCTTCGTATTCCAGTAACTGGAGGAGCAAAAGCAGATGCTGGCTCTTTAAACGGTGCTATTTGTTCAGCCGTTGAGATCATGCAAGCAATGGGATCCTCTATTTGCTCCCTGCTCCCAAGGGTGGAGGTAATACAGACGTTGGTTCTTGAACTTGCTATTGTCGCAGCCCAAGAAAAAGCAATGCTCGATGAATGTATAGCATTGTTGGCATCAACAGCGGCTTTGCAGCTAAGCACTGTTGCTGATAAGAGCTGTTTATTTAGCGAGAGTAAAGTACATATTGCAATTATCAATCTATCAGTTTTTGCAATTGCTTTGAAAACAAGAATCCGCTCAGGAGCAGAGTTTGTGGACGCATCTCATACAAATGAAACAAGGACTGGAAACGGGCTGCCAGTTTTAGGCGGTGGTTAA

Coding sequence (CDS)

ATGGATGTATGCGAATCGGCACGGGCATTTAGAAAGCATACAGTAGCAGCGACGCTAAGACAACCGCTGGTTCCTGCAGAAAAGAACAATGAAGTTATCACACGGTCTTCCTCGCGTTCCAAGAACAAGTCACCTTCTCCTTCATCGTTGTCTGGTTCTCGGCGTTGCCCATCACCAAGCATCACGCGGACGGTTTCTACACCCTCCCAATTGGTTCTTAAAAGAGCTCAATCAGCAGAGAGGAAGCGACCCTCCACACCCCCTTCTCCTTCAAGCCCGGCGACACCGATTCATGGTTCACCAGCAGATGTACAGTTGCTATCGAAAAGAATGATTGGTGGTCGACCTGAGGGTTTATGGCCCTCTACGATGAGAAGTTTGAGCGTCTCCTTCCAATCTGATACAATTTCTATTCCTGTTAGTAAGAAGGAAAAACCCGTGTTATCTTCTCCTTCTGATCGAACTCTGAGGCCTTCTTCCAACTTTCCTTCTAAGCAGGCTGAAACCCAAACTGTTGCACGGAAGCCCACACCAGAAAGAAAGAAGAGTCCTCTTAGAAGTAAAAATGGGCATGACCAATCAGAAAATTTCAAGCCAGTTGATGGGTCACGTACCCAATTCGTAGATCAGCACAGATGGCCAAGTAGAGTTGGTGCGAAAGCATCCTCCAATTCATTGACTTGTAGTGTGGATCTCACTGATAAAAGAATCCCAAGCTTAAATAAGCCCCTCAGAGGAAACGAATTATCTTCTACGAGGGCAACAACGGTCGAGACTATTAACAAATCTTTACAGAGATCGACCAGTGGTGTTATGAGGCTAGCATATGTTGATGGAAGGAGTAGGGAAGAATTTGAGGCAAAATCAGCTGCTGACAATTCAGTGCAGGAGTCTGCAGCTAACAACGTTGTTTCTTCAAGTTTAGCAGGCATAAAAATAACCACAAACCGAGTTGTGAGATATGACTCACCTACTCTTGGCCCACGACCATCTTCACCTTCTAAGACACCAGTATTATCCTCTGTTACTAGAGGAGTTAGCCCATCTCGAATAAGACCATCAACTACACCTCCTCGAGGAATTAGCTCATCACGAATAAGACCTTCAAATTCAACTCAATCCAATGCTTCAACTTCTGTACTTAGCTTCATAGCAGATTTTAAGAAGGGAAAGAAGGCGGCAAGCTATATAGAAGGTGCTCATCAACTACGGCTTCTATACAATAGACATTTGCAGTGGAGATGTGCTAATGCACGGGCAGAGGCTGTTCTGCGCAATCAGGAGGTGAAAGCAGAGAGAACTCTTCTTGGCGTGTGGAATACTACAGTAAATCTGTGGGATTCAGTAATCAGAAAAAGAATTAATCTGCAGCAGCTGAAGCTAGAGCTCAAATTCATCTCAATTATGAATGATCAAATGAGCTGCCTCAACGAATGGGCCGTGCTTGAAAGAAGTCATAATCGATCCTTATCAGGTGTCATAGATGATTTGGAGTCGAGCACTCTTCGTATTCCAGTAACTGGAGGAGCAAAAGCAGATGCTGGCTCTTTAAACGGTGCTATTTGTTCAGCCGTTGAGATCATGCAAGCAATGGGATCCTCTATTTGCTCCCTGCTCCCAAGGGTGGAGGTAATACAGACGTTGGTTCTTGAACTTGCTATTGTCGCAGCCCAAGAAAAAGCAATGCTCGATGAATGTATAGCATTGTTGGCATCAACAGCGGCTTTGCAGCTAAGCACTGTTGCTGATAAGAGCTGTTTATTTAGCGAGAGTAAAGTACATATTGCAATTATCAATCTATCAGTTTTTGCAATTGCTTTGAAAACAAGAATCCGCTCAGGAGCAGAGTTTGTGGACGCATCTCATACAAATGAAACAAGGACTGGAAACGGGCTGCCAGTTTTAGGCGGTGGTTAA

Protein sequence

MDVCESARAFRKHTVAATLRQPLVPAEKNNEVITRSSSRSKNKSPSPSSLSGSRRCPSPSITRTVSTPSQLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPEGLWPSTMRSLSVSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPTPERKKSPLRSKNGHDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRIPSLNKPLRGNELSSTRATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQESAANNVVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTTPPRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCANARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLNEWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICSLLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQLSTVADKSCLFSESKVHIAIINLSVFAIALKTRIRSGAEFVDASHTNETRTGNGLPVLGGG
Homology
BLAST of Lag0033759 vs. NCBI nr
Match: XP_038879190.1 (AUGMIN subunit 8-like [Benincasa hispida] >XP_038879191.1 AUGMIN subunit 8-like [Benincasa hispida] >XP_038879192.1 AUGMIN subunit 8-like [Benincasa hispida])

HSP 1 Score: 946.8 bits (2446), Expect = 9.5e-272
Identity = 517/582 (88.83%), Postives = 538/582 (92.44%), Query Frame = 0

Query: 1   MDVCESARAFRKHTVA----ATLRQPLVPAEKNNEVITRSSSRSKNKSPSPSSLSGSRRC 60
           MDVCESARAFRKHTVA    AT RQPLVPAEKNNEVITRS SRSK  S SPSSLSG RRC
Sbjct: 1   MDVCESARAFRKHTVAAAATATPRQPLVPAEKNNEVITRSPSRSKINSSSPSSLSGPRRC 60

Query: 61  PSPSITRTVSTPSQLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRP 120
           PSPSITRTVST SQLVLKRAQSAERKRPSTPPSP SP TP+HGSPADVQLLSKRMIGGR 
Sbjct: 61  PSPSITRTVSTSSQLVLKRAQSAERKRPSTPPSPPSPGTPVHGSPADVQLLSKRMIGGRA 120

Query: 121 EGLWPSTMRSLSVSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKP 180
           E LWPSTMRSLSVSFQSDTISIPV KKEKPVLSS SDRTLRPSSNFPSKQAETQTVARKP
Sbjct: 121 ESLWPSTMRSLSVSFQSDTISIPVCKKEKPVLSSLSDRTLRPSSNFPSKQAETQTVARKP 180

Query: 181 TPERKKSPLRSKNGHDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKR 240
            PERKKSPLR KNG+DQSEN KPVDGSRTQFVD  RWPSRVGAKASSNSL C+VDLTDKR
Sbjct: 181 MPERKKSPLRGKNGYDQSENSKPVDGSRTQFVDHQRWPSRVGAKASSNSLNCTVDLTDKR 240

Query: 241 IPSLNKPLRGNELSSTRATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQ 300
           +PSL+KPLRG+ LSSTRATT ET+NK LQ+STSGVMRL YVDGRSREEFEA SA DNS+Q
Sbjct: 241 VPSLHKPLRGSGLSSTRATTGETVNKPLQKSTSGVMRLPYVDGRSREEFEANSANDNSMQ 300

Query: 301 ESAANNVVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTT 360
           ESAAN VVSSSLAGIKIT NRV RYDSPTL PRPSSPSKTPVLSSV RGVSPSR+RPST 
Sbjct: 301 ESAANKVVSSSLAGIKITANRVGRYDSPTLCPRPSSPSKTPVLSSVARGVSPSRLRPSTP 360

Query: 361 PPRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCA 420
           PPRG SSSRIRPS+STQSNASTSVL+FIAD+KKGKK ASYIEGAH+LRLLYNRHLQWRCA
Sbjct: 361 PPRGTSSSRIRPSSSTQSNASTSVLTFIADYKKGKKTASYIEGAHKLRLLYNRHLQWRCA 420

Query: 421 NARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCL 480
           NARAEAVLRNQEV AE+TLLGVWNTT+NLWDSVIRKRINLQQLK ELK ISIMNDQMSCL
Sbjct: 421 NARAEAVLRNQEVTAEKTLLGVWNTTLNLWDSVIRKRINLQQLKQELKLISIMNDQMSCL 480

Query: 481 NEWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSIC 540
           NEWAVLERSHNRSLSGVIDDLESSTLR+PVTGGAKADAGSLNGAICSAVE+MQAMGSSIC
Sbjct: 481 NEWAVLERSHNRSLSGVIDDLESSTLRVPVTGGAKADAGSLNGAICSAVEVMQAMGSSIC 540

Query: 541 SLLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQL 579
           SLLPRV+V+QTLV EL IV AQEKAMLDECIALLAS AALQ+
Sbjct: 541 SLLPRVKVMQTLVFELTIVTAQEKAMLDECIALLASRAALQV 582

BLAST of Lag0033759 vs. NCBI nr
Match: KAA0035102.1 (AUGMIN subunit 8-like [Cucumis melo var. makuwa])

HSP 1 Score: 919.5 bits (2375), Expect = 1.6e-263
Identity = 503/584 (86.13%), Postives = 531/584 (90.92%), Query Frame = 0

Query: 1   MDVCESARAFRKHTV---AATLRQPLVPAEKNNEVITRSSSRSKNKSPSPSSLSGSRRCP 60
           MDVCESARAFRKHTV   AAT RQPL PA KNNEVITRS SRSKN S SPSSLSG RRCP
Sbjct: 1   MDVCESARAFRKHTVESAAATPRQPLGPAAKNNEVITRSPSRSKNNSSSPSSLSGPRRCP 60

Query: 61  SPSITRTVSTPSQLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPE 120
           SPSITR VS  S LVLKRAQSAERKRPSTPPSP SPATPIHG+PADVQLLSKRMIGGR E
Sbjct: 61  SPSITRPVSASSPLVLKRAQSAERKRPSTPPSPPSPATPIHGTPADVQLLSKRMIGGRTE 120

Query: 121 GLWPSTMRSLSVSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT 180
            LWPSTMRSLSVSFQSDTISIPV KKEKPVLSSPSDRTLRPSSNF SKQAETQTV+RKPT
Sbjct: 121 SLWPSTMRSLSVSFQSDTISIPVCKKEKPVLSSPSDRTLRPSSNFSSKQAETQTVSRKPT 180

Query: 181 PERKKSPLRSKNGHDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRI 240
           PERKKSPLR KNG+DQ EN KPVDGSRTQF D  RWPSRVGAKASSNSL+C+VDLTDK++
Sbjct: 181 PERKKSPLRGKNGYDQLENSKPVDGSRTQFADHQRWPSRVGAKASSNSLSCTVDLTDKKV 240

Query: 241 PSLNKPLRGNELSSTRATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQE 300
           PSL+KPLRG+ LSSTRATT E +NK  Q+STSGV+RL+YVD RSREEFEA SA DNS+QE
Sbjct: 241 PSLHKPLRGSGLSSTRATTGEIVNKPSQKSTSGVVRLSYVDRRSREEFEANSANDNSMQE 300

Query: 301 SAANNVVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTTP 360
           SAAN VVSSSLAGIKIT NRV RYDSPTL PRPSSPSKTPVLSSV RGVSPSR+RPST P
Sbjct: 301 SAANKVVSSSLAGIKITANRVGRYDSPTLCPRPSSPSKTPVLSSVGRGVSPSRLRPSTPP 360

Query: 361 PRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCAN 420
           P+GISSSR RPSNSTQSNASTSVLSFIAD+KKGKK ASYIEGAH+LRLLYNRHLQWRCAN
Sbjct: 361 PQGISSSRTRPSNSTQSNASTSVLSFIADYKKGKKTASYIEGAHKLRLLYNRHLQWRCAN 420

Query: 421 ARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLN 480
           ARAE VLRNQEV AE+TLLGVWNTT+NLWDSVIRKRINLQQLK ELK ISIMNDQM CLN
Sbjct: 421 ARAEVVLRNQEVIAEKTLLGVWNTTLNLWDSVIRKRINLQQLKQELKLISIMNDQMRCLN 480

Query: 481 EWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICS 540
           EWA+LERSHN+SLSGVIDDLESSTLR+PVTGGAKADAGSL GAICSAVE+MQAMGSSIC 
Sbjct: 481 EWALLERSHNQSLSGVIDDLESSTLRVPVTGGAKADAGSLKGAICSAVEVMQAMGSSICF 540

Query: 541 LLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQLSTV 582
           LLPRV+V+QTLV EL IVAAQEKAMLDECIALLAS AALQ+  +
Sbjct: 541 LLPRVKVMQTLVFELTIVAAQEKAMLDECIALLASRAALQVRMI 584

BLAST of Lag0033759 vs. NCBI nr
Match: TYJ95976.1 (AUGMIN subunit 8-like [Cucumis melo var. makuwa])

HSP 1 Score: 918.7 bits (2373), Expect = 2.8e-263
Identity = 503/584 (86.13%), Postives = 531/584 (90.92%), Query Frame = 0

Query: 1   MDVCESARAFRKHTV---AATLRQPLVPAEKNNEVITRSSSRSKNKSPSPSSLSGSRRCP 60
           MDVCESARAFRKHTV   AAT RQPL PA KNNEVITRS SRSKN S SPSSLSG RRCP
Sbjct: 1   MDVCESARAFRKHTVESAAATPRQPLGPAAKNNEVITRSPSRSKNNSSSPSSLSGPRRCP 60

Query: 61  SPSITRTVSTPSQLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPE 120
           SPSITR VS  SQLVLKRAQSAERKRPSTPPSP SPATPIHG+PADVQLLSKRMIGGR E
Sbjct: 61  SPSITRPVSASSQLVLKRAQSAERKRPSTPPSPPSPATPIHGTPADVQLLSKRMIGGRTE 120

Query: 121 GLWPSTMRSLSVSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT 180
            LWPSTMRSLSVSFQSDTISIPV KKEKPVLSSPSDRTLRPSSNF SKQAETQTV+RKPT
Sbjct: 121 SLWPSTMRSLSVSFQSDTISIPVCKKEKPVLSSPSDRTLRPSSNFSSKQAETQTVSRKPT 180

Query: 181 PERKKSPLRSKNGHDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRI 240
           PERKKSPLR KNG+DQ EN KPVDGSRTQF D  RWPSRVGAKASSNSL+C+VDLTDK++
Sbjct: 181 PERKKSPLRGKNGYDQLENSKPVDGSRTQFADHQRWPSRVGAKASSNSLSCTVDLTDKKV 240

Query: 241 PSLNKPLRGNELSSTRATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQE 300
           PSL+KPLRG+ LSSTRATT E +NK  Q+STSGV+RL+YVD RSREEFEA SA DNS+QE
Sbjct: 241 PSLHKPLRGSGLSSTRATTGEIVNKPSQKSTSGVVRLSYVDRRSREEFEANSANDNSMQE 300

Query: 301 SAANNVVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTTP 360
           SAAN VVSSSLAGIKIT NRV RYDSPTL PRPSSPSKT VLSSV RGVSPSR+RPST P
Sbjct: 301 SAANKVVSSSLAGIKITANRVGRYDSPTLCPRPSSPSKTTVLSSVGRGVSPSRLRPSTPP 360

Query: 361 PRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCAN 420
           P+GISSSR RPSNSTQSNASTSVLSFIAD+KKGKK ASYIEGAH+LRLLYNRHLQWRCAN
Sbjct: 361 PQGISSSRTRPSNSTQSNASTSVLSFIADYKKGKKTASYIEGAHKLRLLYNRHLQWRCAN 420

Query: 421 ARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLN 480
           ARAE VLRNQEV AE+TLLGVWNTT+NLWDSVIRKRINLQQLK ELK ISIMNDQM CLN
Sbjct: 421 ARAEVVLRNQEVIAEKTLLGVWNTTLNLWDSVIRKRINLQQLKQELKLISIMNDQMRCLN 480

Query: 481 EWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICS 540
           EWA+LERSHN+SLSGVIDDLESSTLR+PVTGGAKADAGSL GAICSAVE+MQAMGSSIC 
Sbjct: 481 EWALLERSHNQSLSGVIDDLESSTLRVPVTGGAKADAGSLKGAICSAVEVMQAMGSSICF 540

Query: 541 LLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQLSTV 582
           LLPRV+V+QTLV EL IVAAQEKAMLDECIALLAS AALQ+  +
Sbjct: 541 LLPRVKVMQTLVFELTIVAAQEKAMLDECIALLASRAALQVRMI 584

BLAST of Lag0033759 vs. NCBI nr
Match: XP_008443841.1 (PREDICTED: AUGMIN subunit 8-like [Cucumis melo] >XP_008443842.1 PREDICTED: AUGMIN subunit 8-like [Cucumis melo] >XP_008443843.1 PREDICTED: AUGMIN subunit 8-like [Cucumis melo])

HSP 1 Score: 918.7 bits (2373), Expect = 2.8e-263
Identity = 503/581 (86.57%), Postives = 530/581 (91.22%), Query Frame = 0

Query: 1   MDVCESARAFRKHTV---AATLRQPLVPAEKNNEVITRSSSRSKNKSPSPSSLSGSRRCP 60
           MDVCESARAFRKHTV   AAT RQPL PA KNNEVITRS SRSKN S SPSSLSG RRCP
Sbjct: 1   MDVCESARAFRKHTVESAAATPRQPLGPAAKNNEVITRSPSRSKNNSSSPSSLSGPRRCP 60

Query: 61  SPSITRTVSTPSQLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPE 120
           SPSITR VS  SQLVLKRAQSAERKRPSTPPSP SPATPIHG+PADVQLLSKRMIGGR E
Sbjct: 61  SPSITRPVSASSQLVLKRAQSAERKRPSTPPSPPSPATPIHGTPADVQLLSKRMIGGRTE 120

Query: 121 GLWPSTMRSLSVSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT 180
            LWPSTMRSLSVSFQSDTISIPV KKEKPVLSSPSDRTLRPSSNF SKQAETQTV+RKPT
Sbjct: 121 SLWPSTMRSLSVSFQSDTISIPVCKKEKPVLSSPSDRTLRPSSNFSSKQAETQTVSRKPT 180

Query: 181 PERKKSPLRSKNGHDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRI 240
           PERKKSPLR KNG+DQ EN KPVDGSRTQF D  RWPSRVGAKASSNSL+C+VDLTDK++
Sbjct: 181 PERKKSPLRGKNGYDQLENSKPVDGSRTQFADHQRWPSRVGAKASSNSLSCTVDLTDKKV 240

Query: 241 PSLNKPLRGNELSSTRATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQE 300
           PSL+KPLRG+ LSSTRATT E +NK  Q+STSGV+RL+YVD RSREEFEA SA DNS+QE
Sbjct: 241 PSLHKPLRGSGLSSTRATTGEIVNKPSQKSTSGVVRLSYVDRRSREEFEANSANDNSMQE 300

Query: 301 SAANNVVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTTP 360
           SAAN VVSSSLAGIKIT NRV RYDSPTL PRPSSPSKT VLSSV RGVSPSR+RPST P
Sbjct: 301 SAANKVVSSSLAGIKITANRVGRYDSPTLCPRPSSPSKTTVLSSVGRGVSPSRLRPSTPP 360

Query: 361 PRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCAN 420
           P+GISSSR RPSNSTQSNASTSVLSFIAD+KKGKK ASYIEGAH+LRLLYNRHLQWRCAN
Sbjct: 361 PQGISSSRTRPSNSTQSNASTSVLSFIADYKKGKKTASYIEGAHKLRLLYNRHLQWRCAN 420

Query: 421 ARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLN 480
           ARAE VLRNQEV AE+TLLGVWNTT+NLWDSVIRKRINLQQLK ELK ISIMNDQM CLN
Sbjct: 421 ARAEVVLRNQEVIAEKTLLGVWNTTLNLWDSVIRKRINLQQLKQELKLISIMNDQMRCLN 480

Query: 481 EWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICS 540
           EWA+LERSHN+SLSGVIDDLESSTLR+PVTGGAKADAGSL GAICSAVE+MQAMGSSIC 
Sbjct: 481 EWALLERSHNQSLSGVIDDLESSTLRVPVTGGAKADAGSLKGAICSAVEVMQAMGSSICF 540

Query: 541 LLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQL 579
           LLPRV+V+QTLV EL IVAAQEKAMLDECIALLAS AALQ+
Sbjct: 541 LLPRVKVMQTLVFELTIVAAQEKAMLDECIALLASRAALQV 581

BLAST of Lag0033759 vs. NCBI nr
Match: XP_023515712.1 (AUGMIN subunit 8-like [Cucurbita pepo subsp. pepo] >XP_023515713.1 AUGMIN subunit 8-like [Cucurbita pepo subsp. pepo] >XP_023515714.1 AUGMIN subunit 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 908.7 bits (2347), Expect = 2.9e-260
Identity = 498/581 (85.71%), Postives = 527/581 (90.71%), Query Frame = 0

Query: 1   MDVCESARAFRKHTV---AATLRQPLVPAEKNNEVITRSSSRSKNKSPSPSSLSGSRRCP 60
           MDVCES RAFRKHT+   AAT RQPLVPAEKNNEVITRS SRSKNKSPSPSS SG RRCP
Sbjct: 1   MDVCESVRAFRKHTIAAAAATPRQPLVPAEKNNEVITRSPSRSKNKSPSPSSSSGPRRCP 60

Query: 61  SPSITRTVSTPSQLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPE 120
           SPSITRTVST SQ+VLKRAQSAERKRPSTP SP  PATPIH SPADV+LLSK+ IGGR E
Sbjct: 61  SPSITRTVSTSSQMVLKRAQSAERKRPSTPASPPRPATPIHSSPADVELLSKKRIGGRAE 120

Query: 121 GLWPSTMRSLSVSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT 180
            LWPSTMRSLS SFQSDTIS PV KKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT
Sbjct: 121 SLWPSTMRSLSASFQSDTIS-PVCKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT 180

Query: 181 PERKKSPLRSKNGHDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRI 240
           PERKKSPLR  NGHDQSENFKPVDGSRTQFVD  RW SRVGAKASSNSL+CSVDLTDKR+
Sbjct: 181 PERKKSPLRGLNGHDQSENFKPVDGSRTQFVDHQRWRSRVGAKASSNSLSCSVDLTDKRV 240

Query: 241 PSLNKPLRGNELSSTRATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQE 300
           PSLNKPLRG+ LS TRA T +T+NK LQ+STSG +RL+YVDGR+REEFE  SA DNS+QE
Sbjct: 241 PSLNKPLRGSGLSPTRAITGKTMNKPLQKSTSGDVRLSYVDGRTREEFETTSANDNSMQE 300

Query: 301 SAANNVVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTTP 360
           SAAN +VSSSLAG KI TNRVVRYDSP+LGPRPSSPSK  VLSSV RGVSPSR+RPST P
Sbjct: 301 SAANKLVSSSLAGTKIITNRVVRYDSPSLGPRPSSPSKAQVLSSVARGVSPSRLRPSTPP 360

Query: 361 PRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCAN 420
            RGISSSR RPSNSTQSNASTSVLSFIADFKKGKKAAS IE AHQLRLLYNRHLQWRCAN
Sbjct: 361 SRGISSSRSRPSNSTQSNASTSVLSFIADFKKGKKAASCIEDAHQLRLLYNRHLQWRCAN 420

Query: 421 ARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLN 480
           ARAEAV+RNQEV AERTLLG+WNTT+NLWDSVIRKRI+LQQLKLELK ISIMNDQMS LN
Sbjct: 421 ARAEAVMRNQEVTAERTLLGIWNTTLNLWDSVIRKRIDLQQLKLELKLISIMNDQMSYLN 480

Query: 481 EWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICS 540
           EWAV ERSHNRSLSGVI+DLES TLR+PVTGGA+ADA SLNGAICSAV +MQAMGSSICS
Sbjct: 481 EWAVFERSHNRSLSGVIEDLESRTLRVPVTGGARADACSLNGAICSAVGVMQAMGSSICS 540

Query: 541 LLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQL 579
           LLPRVEV+QTLV EL IVA+QEKAMLDEC+ALLASTAA Q+
Sbjct: 541 LLPRVEVMQTLVFELTIVASQEKAMLDECVALLASTAAFQV 580

BLAST of Lag0033759 vs. ExPASy Swiss-Prot
Match: Q9SUH5 (AUGMIN subunit 8 OS=Arabidopsis thaliana OX=3702 GN=AUG8 PE=1 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 4.5e-123
Identity = 303/613 (49.43%), Postives = 387/613 (63.13%), Query Frame = 0

Query: 18  TLRQPLVPAEKNNEVITRSSSR-----SKNKSPSPSSLSGSRRCPSPSITR-TVSTPSQL 77
           T R+ L+P++KNN V+     R     S+ +SP+P+    + RCPSPS+TR TVS+ SQ 
Sbjct: 7   TTRRRLLPSDKNNAVVATRRPRTMEVSSRYRSPTPTK---NGRCPSPSVTRPTVSSSSQS 66

Query: 78  V-LKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGR-PEGLWPSTMRSLSV 137
           V  KRA SAERKRPSTPPSP+SP+TPI     D+   S+R+  GR PE LWPSTMRSLSV
Sbjct: 67  VAAKRAVSAERKRPSTPPSPTSPSTPIRDLSIDLPASSRRLSTGRLPESLWPSTMRSLSV 126

Query: 138 SFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSK-QAETQTVARKPTPERKKSPLRSK 197
           SFQSD++S+PVSKKE+PV SS  DRTLRPSSN   K +AET +V+RKPTPERK+SPL+ K
Sbjct: 127 SFQSDSVSVPVSKKERPVSSSSGDRTLRPSSNIAQKHKAETTSVSRKPTPERKRSPLKGK 186

Query: 198 NG-HDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRIPSLNKPLRGN 257
           N   D SEN KPVDG  ++ ++QHRWPSR+G K +SNSL  S+DL DK    +     G 
Sbjct: 187 NNVSDLSENSKPVDGPHSRLIEQHRWPSRIGGKITSNSLNRSLDLGDKASRGIPTSGPGM 246

Query: 258 ELSSTR-ATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQESAANNVVSS 317
             S  R +  + + ++ L +++S       +          KS  +N  + S A  ++S+
Sbjct: 247 GPSLRRMSLPLSSSSRPLHKTSSNTSSYGGLVS------PTKSEDNNIARTSGAQRLLSA 306

Query: 318 -SLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLS--------SVTRGVSPSR------- 377
            SL    + T     +  P  G RP+SPS+T  LS        S +RGVSPSR       
Sbjct: 307 GSLDRATLATAVARLHPLPAPGSRPASPSRTSFLSSSSISRGMSTSRGVSPSRGLSPSRG 366

Query: 378 ------------------------IRPSTTPPRGISSSRIR-PSNSTQSNASTSVLSFIA 437
                                    RPST P RGIS SRIR  + STQS+ +TSVLSFI 
Sbjct: 367 LSPTRGLSPSRGLSPSRGTNTSCFARPSTPPSRGISPSRIRQTTTSTQSSTTTSVLSFIT 426

Query: 438 DFKKGKKAASYIEGAHQLRLLYNRHLQWRCANARAEAVLRNQEVKAERTLLGVWNTTVNL 497
           D KKGKK ASYIE  HQLRLL+NR+LQWR A ARAE+V+  Q + +E TL  VW+    L
Sbjct: 427 DVKKGKK-ASYIEDVHQLRLLHNRYLQWRFAIARAESVMYIQRLTSEETLFNVWHAISEL 486

Query: 498 WDSVIRKRINLQQLKLELKFISIMNDQMSCLNEWAVLERSHNRSLSGVIDDLESSTLRIP 557
            D V R+RI LQQLKLE+K  S++NDQM  L +WA LER H  SL G I DLE++TLR+P
Sbjct: 487 QDHVTRQRIGLQQLKLEIKLNSLLNDQMVSLEDWATLERDHVSSLVGAISDLEANTLRLP 546

Query: 558 VTGGAKADAGSLNGAICSAVEIMQAMGSSICSLLPRVEVIQTLVLELAIVAAQEKAMLDE 579
            TGG KAD  SL  A+ SA+++MQAMGSSI SLL +VE +  +V ELA+V  +E +M  +
Sbjct: 547 ATGGTKADTESLKAAMSSALDVMQAMGSSIWSLLSKVEEMNIMVTELAVVVTKESSMQGK 606

BLAST of Lag0033759 vs. ExPASy Swiss-Prot
Match: F4INP9 (QWRF motif-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=QWRF4 PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.6e-104
Identity = 273/600 (45.50%), Postives = 369/600 (61.50%), Query Frame = 0

Query: 16  AATLRQPLVPAEKNN--EVITRSSS---RSKNKSPSPSSLSGSRRCPSPSITRTV-STPS 75
           A + R PL P+EKNN   V  R+ +    S+ +SP+P+    +RRCPSP +TRT  S+  
Sbjct: 20  ATSPRPPLAPSEKNNVGSVTRRARTMEVSSRYRSPTPTK---TRRCPSPIVTRTAPSSSP 79

Query: 76  QLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGR-PEGLWPSTMRSLS 135
           +  LKRA SAER R      PS+P TP+     D+ + S+R+  GR PE LWPSTMRSLS
Sbjct: 80  ESFLKRAVSAERNR-----GPSTPTTPVSDVLVDLPVSSRRLSTGRLPESLWPSTMRSLS 139

Query: 136 VSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSN--FPSKQAETQTVARKPTPERKKSPLR 195
           VSFQSD++S+PVSKKEKP+++S +DRTLRPSS+     +Q+ET +V RK TPERK+SPL+
Sbjct: 140 VSFQSDSVSVPVSKKEKPLVTSSTDRTLRPSSSNIAHKQQSETTSVTRKQTPERKRSPLK 199

Query: 196 SKN-GHDQSENFKPVDGSRTQFV-DQHRWPSRVGAKASSNSLTCSVDLTDKRIPSLNKPL 255
            KN    QSEN KP+DGS +  +  QHRW  R+           S DL DK +  ++ PL
Sbjct: 200 GKNVSPGQSENSKPMDGSHSMLIPPQHRWSGRIRGNR-------SFDLGDKAVRRVSLPL 259

Query: 256 RGNELSSTRATTVETINKSL--QRSTSGVMRLAYVDGRSREEFEAKSAADNSVQESAANN 315
                           NKS   ++S+S + RL       R E  + + +++S    +  +
Sbjct: 260 S---------------NKSSRHKKSSSDITRLFSCYDNGRLEVSSSTTSEDSSSTESLKH 319

Query: 316 VVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSR----------IR 375
             +SSL  +   +    R  SP+     SS S      S +RGVSP R          +R
Sbjct: 320 FSTSSLPRLHPMSAPGSRTASPSRSSFSSSSSSNSRGMSPSRGVSPMRGLSPVGNRSLVR 379

Query: 376 PSTTPPRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQ 435
            ST P RG+S SRIR   +  S+ +TSVLSFIAD KKGKK A+YIE  HQLRLLYNR+ Q
Sbjct: 380 SSTPPSRGVSPSRIR-QTAQSSSTNTSVLSFIADVKKGKK-ATYIEDVHQLRLLYNRYSQ 439

Query: 436 WRCANARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQ 495
           WR ANARAE V   Q + A+ TL  VW+   +L D V  +RI LQQLKLE+K  SI+NDQ
Sbjct: 440 WRFANARAEGVSYVQSLIAKETLYNVWHAISDLRDLVTTQRICLQQLKLEIKLRSILNDQ 499

Query: 496 MSCLNEWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMG 555
           M CL +WA++ER H  SL+G I DLE++TLR+P+ GG KAD GSL  A+ SA+++MQ+MG
Sbjct: 500 MVCLEDWAMVEREHISSLAGAIGDLEANTLRLPLAGGTKADLGSLKLAMSSALDVMQSMG 559

Query: 556 SSICSLLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQLSTVADKSCLFSESK 593
           SSI SL  ++E +  LV +LA++A  E  +LD+C  LLASTA +++   + K+ L  + +
Sbjct: 560 SSIWSLHSQMEEMNKLVSDLAVIAKTENFLLDKCENLLASTAVMEIEERSLKTHLIQKKQ 587

BLAST of Lag0033759 vs. ExPASy Swiss-Prot
Match: Q94AI1 (QWRF motif-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=QWRF2 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 6.2e-48
Identity = 210/660 (31.82%), Postives = 308/660 (46.67%), Query Frame = 0

Query: 13  HTVAATLRQPLVPAEKNNEVITRSSS---RSKNKSPSPSSLSGSRRCPSPSITRTVSTPS 72
           H+V++T           +   + SSS   R+  + PSPS L    R  + S + ++ TPS
Sbjct: 47  HSVSSTTTTTTTTTTTTSSSSSSSSSAILRTSKRYPSPSPLLS--RSTTNSASNSIKTPS 106

Query: 73  QLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPEGLWPSTMRSLSV 132
            L+ KR+QS +R+RPS                 ++   +K +I         ++ RSLSV
Sbjct: 107 -LLPKRSQSVDRRRPSAVSVTVG---------TEMSAATKMLI---------TSTRSLSV 166

Query: 133 SFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPTPERKKS-PLRSK 192
           SFQ +  S+P+SKK+        + T  P S+            RK TPER++S P+R  
Sbjct: 167 SFQGEAFSLPISKKK--------ETTSTPVSH------------RKSTPERRRSTPVR-- 226

Query: 193 NGHDQSENFKPVDGSRTQFVDQHRWP--SRVGAKAS------SNSLTCSVDLTDKRIPSL 252
              DQ EN KP        VDQ RWP  SR G   S      S SL C  D        +
Sbjct: 227 ---DQRENSKP--------VDQQRWPGASRRGNSESVVPNSLSRSLDCGSDRGKLGSGFV 286

Query: 253 NKPLRGNEL--SSTRATTVETINKSL--------------QRSTSGVMRLAYVDGRSREE 312
            + +  N +   S R +    ++  L              +R  +G+      D  + + 
Sbjct: 287 GRSMLHNSMIDESPRVSVNGRLSLDLGGRDEYLDIGDDIQRRPNNGLTSSVSCDFTASDT 346

Query: 313 FEAKSAADNSVQE--SAANNVVSSS-------LAGIKI---TTNRVVRYDSPTLGPRPSS 372
               S + N VQE  S  N  +S S       +A  +    T +R+ R   P   P  SS
Sbjct: 347 DSVSSGSTNGVQECGSGVNGEISKSKSLPRNIMASARFWQETNSRLRRLQDPG-SPLSSS 406

Query: 373 PS-----------------------------KTPVLSSVTRGVSPSRIRPSTT--PPRGI 432
           P                               +PV  S  R  SPS++  +TT  P R +
Sbjct: 407 PGLKTSSISSKFGLSKRFSSDAVPLSSPRGMASPVRGSAIRSASPSKLWATTTSSPARAL 466

Query: 433 SS-SRIRPSNSTQSNA-----STSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRC 492
           SS SR R   S Q NA     + S+LSF AD ++GK     +  AH LRLLYNR LQWR 
Sbjct: 467 SSPSRARNGVSDQMNAYNRNNTPSILSFSADIRRGKIGEDRVMDAHLLRLLYNRDLQWRF 526

Query: 493 ANARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSC 552
            NARA++ +  Q + AE+ L   W +   L  SV  KRI L  L+ +LK  SI+  QM  
Sbjct: 527 VNARADSTVMVQRLNAEKNLWNAWVSISELRHSVTLKRIKLLLLRQKLKLASILRGQMGF 586

Query: 553 LNEWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSI 596
           L EW++L+R H+ SLSG  + L++STLR+P+ G    D   L  A+ SAV++MQAM SSI
Sbjct: 587 LEEWSLLDRDHSSSLSGATESLKASTLRLPIVGKTVVDIQDLKHAVSSAVDVMQAMSSSI 646

BLAST of Lag0033759 vs. ExPASy Swiss-Prot
Match: Q8GXD9 (Protein SNOWY COTYLEDON 3 OS=Arabidopsis thaliana OX=3702 GN=SCO3 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 4.0e-47
Identity = 204/647 (31.53%), Postives = 306/647 (47.30%), Query Frame = 0

Query: 23  LVPAEKNNEVITRSSSRSKNKSPSPS-SLSGSRRCPSPSITRTVSTPSQLVL------KR 82
           L P+  ++   T +++ S + S S S  L  S+R PSP ++RT ++ S LV       KR
Sbjct: 52  LSPSPSHSTTTTTTTATSTSTSSSSSVILRSSKRYPSPLLSRTTNSASNLVYTPSSLPKR 111

Query: 83  AQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPEGLWPSTMRSLSVSFQSDT 142
           +QS +R+RPS           +  +  ++   +K +I         ++ RSLSVSFQ + 
Sbjct: 112 SQSVDRRRPSA----------VSDTRTEMSAATKMLI---------TSTRSLSVSFQGEA 171

Query: 143 ISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPTPERKK-SPLRSKNGHDQS 202
            S P+SKK                     K+  T    RK TPER++ +P+R     DQ 
Sbjct: 172 FSFPISKK---------------------KETATPVSHRKCTPERRRATPVR-----DQR 231

Query: 203 ENFKPVDGSRTQFVDQHRWP--SRVGAKAS--SNSLTCSVDLTDKRIPSLNKPLRGNEL- 262
           EN KP        VDQ  WP  SR G+  S   NSL+ SVD        L     G  + 
Sbjct: 232 ENSKP--------VDQQLWPGASRRGSSESVVPNSLSRSVDSDSDDGRKLGSGFVGRSML 291

Query: 263 ---SSTRAT----------------TVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSA 322
               S+R +                 +   NK+ Q +   +      D  + +     S 
Sbjct: 292 QHSQSSRVSGDGRLNLGFVGGDGMLEMRDENKARQSTHPRLASSVSCDFTASDTDSVSSG 351

Query: 323 ADNSVQE------SAANNVVSSSLAGIKI---TTNRVVRYDSPTLGPRPSSPSK------ 382
           + N   E      S   ++  + +A  K    T +R+ R   P   P+ SSPS       
Sbjct: 352 STNGAHECGSGEVSKTRSLPRNGMASTKFWQETNSRLRRMQDPG-SPQCSSPSSRISSIS 411

Query: 383 ------------TPVLSS----------VTRGVSPSRIRPSTT--PPRGISS-SRIRPSN 442
                       +P+ SS           TR  SPS++  + T  P R  SS SR+R   
Sbjct: 412 SKFSQSKRFSSDSPLTSSPRGMTSPIRGATRPASPSKLWATATSAPARTSSSPSRVRNGV 471

Query: 443 STQSNAST----SVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCANARAEAVLRN 502
           S Q NA      S+L F AD ++GK     +  AH LRLLYNR LQWR ANARA++ L  
Sbjct: 472 SEQMNAYNRTLPSILCFSADIRRGKIGEDRVMDAHLLRLLYNRDLQWRFANARADSTLMV 531

Query: 503 QEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLNEWAVLERSH 562
           Q + AE+ L   W +   L  SV  KRI L  ++ +LK  SI+ +QM  L EW++L+R+H
Sbjct: 532 QRLSAEKILWNAWVSISELRHSVTLKRIKLLLMRQKLKLASILKEQMCYLEEWSLLDRNH 591

Query: 563 NRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICSLLPRVEVIQ 594
           + SLSG  + L++STLR+PV+G A  D   L  A+ SAV++M AM SSI SL  +VE + 
Sbjct: 592 SNSLSGATEALKASTLRLPVSGKAVVDIQDLKHAVSSAVDVMHAMVSSIFSLTSKVEEMN 644

BLAST of Lag0033759 vs. ExPASy Swiss-Prot
Match: F4K4M0 (QWRF motif-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=QWRF9 PE=3 SV=2)

HSP 1 Score: 176.8 bits (447), Expect = 7.8e-43
Identity = 161/446 (36.10%), Postives = 232/446 (52.02%), Query Frame = 0

Query: 140 VSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPTPERKKSPLRSKNGHDQSENFKP 199
           VSK E+ +L+S         S F S QA++ T       +   S   SK+G  + E  K 
Sbjct: 89  VSKAERMLLTS-------GRSLFASFQADSFTPGTLERRKTTSSATISKSGGGKQEKLKL 148

Query: 200 VDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRIPSLNKPLRGNELSSTRATTVET 259
            D          +WP  +     S+    SVD TD R     K L G+     RA     
Sbjct: 149 SD----------QWPRSLQPSCLSSR---SVDFTDTR-----KKLIGSGNGVARALQDSM 208

Query: 260 INK---SLQRSTSGVMRLAYV-DGRSREEFEAKSAADNSVQESAANNVVSSSLAGIKITT 319
           ++    S +R TS  +    V  G S    +   A  N V+   + + +  S  G+    
Sbjct: 209 VSNRPVSRERITSVDLETESVSSGSSNGRGKMLPARGNVVKARVSQDRLEPSSHGL---- 268

Query: 320 NRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTTPPRGIS-SSRIRPSNSTQS 379
            R +  DS  L P+ ++   +P  +S+ RG+SPSR      PPRG+S S R+ P     S
Sbjct: 269 -RKISVDSSVLSPKEANSLSSPRGTSIARGLSPSR---EVVPPRGVSPSDRMSPLRVRSS 328

Query: 380 -NASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCANARAEAVLRNQEVKAER 439
            + +T ++   A   K K   + +  AH LRLL++R LQW+ ANARA AV+ +Q+++ ER
Sbjct: 329 LSKNTPLIPHFAVDGKEKIRDNGVADAHLLRLLHSRLLQWQFANARANAVISSQKMREER 388

Query: 440 TLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLNEWAVLERSHNRSLSGV 499
            L   W +  NL++SV  KRI +Q LK  LK ISI+N QM  L EW V++R++  SL G 
Sbjct: 389 RLYNAWRSISNLYNSVSMKRIEMQHLKQNLKLISILNMQMGHLEEWLVIDRNYMGSLVGA 448

Query: 500 IDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICSLLPRVEVIQTLVLELA 559
            + L+ STL +PV  GA  +  S+  AICSAV++MQAM SSIC LLP+V  I +L  EL 
Sbjct: 449 AEALKGSTLCLPVDCGAMVNVQSVKDAICSAVDVMQAMASSICLLLPKVGKISSLAAELG 501

Query: 560 IVAAQEKAMLDECIALLASTAALQLS 580
            V A+++ MLD C  LL + +ALQ++
Sbjct: 509 RVNAKDEGMLDVCRDLLNTISALQVT 501

BLAST of Lag0033759 vs. ExPASy TrEMBL
Match: A0A5A7SV33 (AUGMIN subunit 8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G001770 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 7.9e-264
Identity = 503/584 (86.13%), Postives = 531/584 (90.92%), Query Frame = 0

Query: 1   MDVCESARAFRKHTV---AATLRQPLVPAEKNNEVITRSSSRSKNKSPSPSSLSGSRRCP 60
           MDVCESARAFRKHTV   AAT RQPL PA KNNEVITRS SRSKN S SPSSLSG RRCP
Sbjct: 1   MDVCESARAFRKHTVESAAATPRQPLGPAAKNNEVITRSPSRSKNNSSSPSSLSGPRRCP 60

Query: 61  SPSITRTVSTPSQLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPE 120
           SPSITR VS  S LVLKRAQSAERKRPSTPPSP SPATPIHG+PADVQLLSKRMIGGR E
Sbjct: 61  SPSITRPVSASSPLVLKRAQSAERKRPSTPPSPPSPATPIHGTPADVQLLSKRMIGGRTE 120

Query: 121 GLWPSTMRSLSVSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT 180
            LWPSTMRSLSVSFQSDTISIPV KKEKPVLSSPSDRTLRPSSNF SKQAETQTV+RKPT
Sbjct: 121 SLWPSTMRSLSVSFQSDTISIPVCKKEKPVLSSPSDRTLRPSSNFSSKQAETQTVSRKPT 180

Query: 181 PERKKSPLRSKNGHDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRI 240
           PERKKSPLR KNG+DQ EN KPVDGSRTQF D  RWPSRVGAKASSNSL+C+VDLTDK++
Sbjct: 181 PERKKSPLRGKNGYDQLENSKPVDGSRTQFADHQRWPSRVGAKASSNSLSCTVDLTDKKV 240

Query: 241 PSLNKPLRGNELSSTRATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQE 300
           PSL+KPLRG+ LSSTRATT E +NK  Q+STSGV+RL+YVD RSREEFEA SA DNS+QE
Sbjct: 241 PSLHKPLRGSGLSSTRATTGEIVNKPSQKSTSGVVRLSYVDRRSREEFEANSANDNSMQE 300

Query: 301 SAANNVVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTTP 360
           SAAN VVSSSLAGIKIT NRV RYDSPTL PRPSSPSKTPVLSSV RGVSPSR+RPST P
Sbjct: 301 SAANKVVSSSLAGIKITANRVGRYDSPTLCPRPSSPSKTPVLSSVGRGVSPSRLRPSTPP 360

Query: 361 PRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCAN 420
           P+GISSSR RPSNSTQSNASTSVLSFIAD+KKGKK ASYIEGAH+LRLLYNRHLQWRCAN
Sbjct: 361 PQGISSSRTRPSNSTQSNASTSVLSFIADYKKGKKTASYIEGAHKLRLLYNRHLQWRCAN 420

Query: 421 ARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLN 480
           ARAE VLRNQEV AE+TLLGVWNTT+NLWDSVIRKRINLQQLK ELK ISIMNDQM CLN
Sbjct: 421 ARAEVVLRNQEVIAEKTLLGVWNTTLNLWDSVIRKRINLQQLKQELKLISIMNDQMRCLN 480

Query: 481 EWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICS 540
           EWA+LERSHN+SLSGVIDDLESSTLR+PVTGGAKADAGSL GAICSAVE+MQAMGSSIC 
Sbjct: 481 EWALLERSHNQSLSGVIDDLESSTLRVPVTGGAKADAGSLKGAICSAVEVMQAMGSSICF 540

Query: 541 LLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQLSTV 582
           LLPRV+V+QTLV EL IVAAQEKAMLDECIALLAS AALQ+  +
Sbjct: 541 LLPRVKVMQTLVFELTIVAAQEKAMLDECIALLASRAALQVRMI 584

BLAST of Lag0033759 vs. ExPASy TrEMBL
Match: A0A1S3B9S2 (AUGMIN subunit 8-like OS=Cucumis melo OX=3656 GN=LOC103487338 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.3e-263
Identity = 503/581 (86.57%), Postives = 530/581 (91.22%), Query Frame = 0

Query: 1   MDVCESARAFRKHTV---AATLRQPLVPAEKNNEVITRSSSRSKNKSPSPSSLSGSRRCP 60
           MDVCESARAFRKHTV   AAT RQPL PA KNNEVITRS SRSKN S SPSSLSG RRCP
Sbjct: 1   MDVCESARAFRKHTVESAAATPRQPLGPAAKNNEVITRSPSRSKNNSSSPSSLSGPRRCP 60

Query: 61  SPSITRTVSTPSQLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPE 120
           SPSITR VS  SQLVLKRAQSAERKRPSTPPSP SPATPIHG+PADVQLLSKRMIGGR E
Sbjct: 61  SPSITRPVSASSQLVLKRAQSAERKRPSTPPSPPSPATPIHGTPADVQLLSKRMIGGRTE 120

Query: 121 GLWPSTMRSLSVSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT 180
            LWPSTMRSLSVSFQSDTISIPV KKEKPVLSSPSDRTLRPSSNF SKQAETQTV+RKPT
Sbjct: 121 SLWPSTMRSLSVSFQSDTISIPVCKKEKPVLSSPSDRTLRPSSNFSSKQAETQTVSRKPT 180

Query: 181 PERKKSPLRSKNGHDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRI 240
           PERKKSPLR KNG+DQ EN KPVDGSRTQF D  RWPSRVGAKASSNSL+C+VDLTDK++
Sbjct: 181 PERKKSPLRGKNGYDQLENSKPVDGSRTQFADHQRWPSRVGAKASSNSLSCTVDLTDKKV 240

Query: 241 PSLNKPLRGNELSSTRATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQE 300
           PSL+KPLRG+ LSSTRATT E +NK  Q+STSGV+RL+YVD RSREEFEA SA DNS+QE
Sbjct: 241 PSLHKPLRGSGLSSTRATTGEIVNKPSQKSTSGVVRLSYVDRRSREEFEANSANDNSMQE 300

Query: 301 SAANNVVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTTP 360
           SAAN VVSSSLAGIKIT NRV RYDSPTL PRPSSPSKT VLSSV RGVSPSR+RPST P
Sbjct: 301 SAANKVVSSSLAGIKITANRVGRYDSPTLCPRPSSPSKTTVLSSVGRGVSPSRLRPSTPP 360

Query: 361 PRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCAN 420
           P+GISSSR RPSNSTQSNASTSVLSFIAD+KKGKK ASYIEGAH+LRLLYNRHLQWRCAN
Sbjct: 361 PQGISSSRTRPSNSTQSNASTSVLSFIADYKKGKKTASYIEGAHKLRLLYNRHLQWRCAN 420

Query: 421 ARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLN 480
           ARAE VLRNQEV AE+TLLGVWNTT+NLWDSVIRKRINLQQLK ELK ISIMNDQM CLN
Sbjct: 421 ARAEVVLRNQEVIAEKTLLGVWNTTLNLWDSVIRKRINLQQLKQELKLISIMNDQMRCLN 480

Query: 481 EWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICS 540
           EWA+LERSHN+SLSGVIDDLESSTLR+PVTGGAKADAGSL GAICSAVE+MQAMGSSIC 
Sbjct: 481 EWALLERSHNQSLSGVIDDLESSTLRVPVTGGAKADAGSLKGAICSAVEVMQAMGSSICF 540

Query: 541 LLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQL 579
           LLPRV+V+QTLV EL IVAAQEKAMLDECIALLAS AALQ+
Sbjct: 541 LLPRVKVMQTLVFELTIVAAQEKAMLDECIALLASRAALQV 581

BLAST of Lag0033759 vs. ExPASy TrEMBL
Match: A0A5D3BAQ4 (AUGMIN subunit 8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold271G00460 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.3e-263
Identity = 503/584 (86.13%), Postives = 531/584 (90.92%), Query Frame = 0

Query: 1   MDVCESARAFRKHTV---AATLRQPLVPAEKNNEVITRSSSRSKNKSPSPSSLSGSRRCP 60
           MDVCESARAFRKHTV   AAT RQPL PA KNNEVITRS SRSKN S SPSSLSG RRCP
Sbjct: 1   MDVCESARAFRKHTVESAAATPRQPLGPAAKNNEVITRSPSRSKNNSSSPSSLSGPRRCP 60

Query: 61  SPSITRTVSTPSQLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPE 120
           SPSITR VS  SQLVLKRAQSAERKRPSTPPSP SPATPIHG+PADVQLLSKRMIGGR E
Sbjct: 61  SPSITRPVSASSQLVLKRAQSAERKRPSTPPSPPSPATPIHGTPADVQLLSKRMIGGRTE 120

Query: 121 GLWPSTMRSLSVSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT 180
            LWPSTMRSLSVSFQSDTISIPV KKEKPVLSSPSDRTLRPSSNF SKQAETQTV+RKPT
Sbjct: 121 SLWPSTMRSLSVSFQSDTISIPVCKKEKPVLSSPSDRTLRPSSNFSSKQAETQTVSRKPT 180

Query: 181 PERKKSPLRSKNGHDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRI 240
           PERKKSPLR KNG+DQ EN KPVDGSRTQF D  RWPSRVGAKASSNSL+C+VDLTDK++
Sbjct: 181 PERKKSPLRGKNGYDQLENSKPVDGSRTQFADHQRWPSRVGAKASSNSLSCTVDLTDKKV 240

Query: 241 PSLNKPLRGNELSSTRATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQE 300
           PSL+KPLRG+ LSSTRATT E +NK  Q+STSGV+RL+YVD RSREEFEA SA DNS+QE
Sbjct: 241 PSLHKPLRGSGLSSTRATTGEIVNKPSQKSTSGVVRLSYVDRRSREEFEANSANDNSMQE 300

Query: 301 SAANNVVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTTP 360
           SAAN VVSSSLAGIKIT NRV RYDSPTL PRPSSPSKT VLSSV RGVSPSR+RPST P
Sbjct: 301 SAANKVVSSSLAGIKITANRVGRYDSPTLCPRPSSPSKTTVLSSVGRGVSPSRLRPSTPP 360

Query: 361 PRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCAN 420
           P+GISSSR RPSNSTQSNASTSVLSFIAD+KKGKK ASYIEGAH+LRLLYNRHLQWRCAN
Sbjct: 361 PQGISSSRTRPSNSTQSNASTSVLSFIADYKKGKKTASYIEGAHKLRLLYNRHLQWRCAN 420

Query: 421 ARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLN 480
           ARAE VLRNQEV AE+TLLGVWNTT+NLWDSVIRKRINLQQLK ELK ISIMNDQM CLN
Sbjct: 421 ARAEVVLRNQEVIAEKTLLGVWNTTLNLWDSVIRKRINLQQLKQELKLISIMNDQMRCLN 480

Query: 481 EWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICS 540
           EWA+LERSHN+SLSGVIDDLESSTLR+PVTGGAKADAGSL GAICSAVE+MQAMGSSIC 
Sbjct: 481 EWALLERSHNQSLSGVIDDLESSTLRVPVTGGAKADAGSLKGAICSAVEVMQAMGSSICF 540

Query: 541 LLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQLSTV 582
           LLPRV+V+QTLV EL IVAAQEKAMLDECIALLAS AALQ+  +
Sbjct: 541 LLPRVKVMQTLVFELTIVAAQEKAMLDECIALLASRAALQVRMI 584

BLAST of Lag0033759 vs. ExPASy TrEMBL
Match: A0A6J1JLX4 (AUGMIN subunit 8-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485765 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 3.1e-260
Identity = 497/579 (85.84%), Postives = 527/579 (91.02%), Query Frame = 0

Query: 1   MDVCESARAFRKHTV-AATLRQPLVPAEKNNEVITRSSSRSKNKSPSPSSLSGSRRCPSP 60
           MDVCES RAFRKHT+ AAT RQPLVPAEKNNEVITRS SRSKNKSPSPSS SG RRCPSP
Sbjct: 1   MDVCESVRAFRKHTIAAATPRQPLVPAEKNNEVITRSPSRSKNKSPSPSSSSGPRRCPSP 60

Query: 61  SITRTVSTPSQLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPEGL 120
           SITRTVST SQ+VLKRAQSAERKRPSTPPSP  PATPIH SPADV+LLSK+ IGGR E L
Sbjct: 61  SITRTVSTSSQMVLKRAQSAERKRPSTPPSPPRPATPIHSSPADVELLSKKRIGGRAESL 120

Query: 121 WPSTMRSLSVSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPTPE 180
           WPSTMRSL  SFQSDTIS PV KKEKPVLSS SDRTLRPSSNFPSKQAETQTVARKPTPE
Sbjct: 121 WPSTMRSLGASFQSDTIS-PVCKKEKPVLSSHSDRTLRPSSNFPSKQAETQTVARKPTPE 180

Query: 181 RKKSPLRSKNGHDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRIPS 240
           RKKSPLR  NGHDQSENFKPVDGSRTQFVD  RW SRVGAKASSNSL+CSVDL+DKR+PS
Sbjct: 181 RKKSPLRGLNGHDQSENFKPVDGSRTQFVDHQRWRSRVGAKASSNSLSCSVDLSDKRVPS 240

Query: 241 LNKPLRGNELSSTRATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQESA 300
           LNKPLRG+ LS TRA T +T+NK LQ+STSG +RL+YVDGR+REEFE  SA DNS+QESA
Sbjct: 241 LNKPLRGSGLSPTRAITGKTMNKPLQKSTSGDVRLSYVDGRTREEFETTSANDNSMQESA 300

Query: 301 ANNVVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTTPPR 360
           AN +VSSSLAG KI TNRVVRYDSP+LGPRPSSPSK  VLSSV RGVSPSR+RPST P R
Sbjct: 301 ANKMVSSSLAGTKIITNRVVRYDSPSLGPRPSSPSKAQVLSSVARGVSPSRLRPSTPPSR 360

Query: 361 GISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCANAR 420
           GISSSR RPSNSTQSNASTSVLSFIADFKKGKKAAS IE AHQLRLLYNRHLQWRCANAR
Sbjct: 361 GISSSRSRPSNSTQSNASTSVLSFIADFKKGKKAASCIEDAHQLRLLYNRHLQWRCANAR 420

Query: 421 AEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLNEW 480
           AEAV+RNQEV AERTLLG+WNTT+NLWDSVIRKRI+LQQLKLELK ISIMNDQMS LNEW
Sbjct: 421 AEAVMRNQEVTAERTLLGIWNTTLNLWDSVIRKRIDLQQLKLELKLISIMNDQMSYLNEW 480

Query: 481 AVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICSLL 540
           AVLERSHNRSLSGVI+DLES TLR+PVTGGA+ADA SLNGAICSAV +MQAMGSSICSLL
Sbjct: 481 AVLERSHNRSLSGVIEDLESRTLRVPVTGGARADACSLNGAICSAVGVMQAMGSSICSLL 540

Query: 541 PRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQL 579
           PRVEV+QTLV EL IVA+QEKAMLDEC+ALLASTAA Q+
Sbjct: 541 PRVEVMQTLVFELTIVASQEKAMLDECVALLASTAAFQV 578

BLAST of Lag0033759 vs. ExPASy TrEMBL
Match: A0A6J1E6M8 (AUGMIN subunit 8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429893 PE=3 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 6.9e-260
Identity = 496/581 (85.37%), Postives = 527/581 (90.71%), Query Frame = 0

Query: 1   MDVCESARAFRKHTV---AATLRQPLVPAEKNNEVITRSSSRSKNKSPSPSSLSGSRRCP 60
           MDVCES RAFRKHT+   AAT RQPLVPAEKNNEVITRS SRSKNKSPSPSS SG RRCP
Sbjct: 1   MDVCESVRAFRKHTIAAAAATPRQPLVPAEKNNEVITRSPSRSKNKSPSPSSSSGPRRCP 60

Query: 61  SPSITRTVSTPSQLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPE 120
           SPSITRTVST SQ+VLKRAQSAERKRPSTPPSP  PATPIH SPADV+LLSK+  GGR E
Sbjct: 61  SPSITRTVSTSSQMVLKRAQSAERKRPSTPPSPPRPATPIHSSPADVELLSKKRTGGRAE 120

Query: 121 GLWPSTMRSLSVSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT 180
            LWPSTMRSLS SFQSDTIS PV KKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT
Sbjct: 121 SLWPSTMRSLSASFQSDTIS-PVCKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPT 180

Query: 181 PERKKSPLRSKNGHDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRI 240
           PERKKSPLR  NGHDQSENFKPVDGSRTQFVD  RW +RVGAKASSNSL+CS+DLTDKR+
Sbjct: 181 PERKKSPLRGLNGHDQSENFKPVDGSRTQFVDHQRWRTRVGAKASSNSLSCSMDLTDKRV 240

Query: 241 PSLNKPLRGNELSSTRATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQE 300
           PSLNKPLRGN LS TRA T +T+NK LQ+STSG +RL+YVDGR+REEFE  SA D+S+QE
Sbjct: 241 PSLNKPLRGNGLSPTRAITGKTMNKPLQKSTSGDVRLSYVDGRTREEFETTSANDSSMQE 300

Query: 301 SAANNVVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSRIRPSTTP 360
           SAAN +VSSSLAG KI TNRVVRYDSP+LGPRPSSPSK  VLSSV RGVSPSR+RPST P
Sbjct: 301 SAANKLVSSSLAGTKIITNRVVRYDSPSLGPRPSSPSKAQVLSSVARGVSPSRLRPSTPP 360

Query: 361 PRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRCAN 420
            RGISSSR RPSNSTQSNASTSVLSFIADFKKGKKAAS IE AHQLRLLYNRHLQWRCAN
Sbjct: 361 SRGISSSRSRPSNSTQSNASTSVLSFIADFKKGKKAASCIEDAHQLRLLYNRHLQWRCAN 420

Query: 421 ARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSCLN 480
           ARAEAV+RNQEV AERTLLG+WNTT+NLWDSVIRKRI+LQQLKLELK ISIMNDQMS LN
Sbjct: 421 ARAEAVMRNQEVTAERTLLGIWNTTLNLWDSVIRKRIDLQQLKLELKLISIMNDQMSYLN 480

Query: 481 EWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSICS 540
           EWAVLERSHNRSLSGV +DLES TLR+PVTGGA+ADA SLNGAICSAV +MQAMGSSICS
Sbjct: 481 EWAVLERSHNRSLSGVKEDLESRTLRVPVTGGARADACSLNGAICSAVGVMQAMGSSICS 540

Query: 541 LLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQL 579
           LLPRVEV+QTLV EL IVA+QEKAMLDEC+ALLASTAA Q+
Sbjct: 541 LLPRVEVMQTLVFELTIVASQEKAMLDECVALLASTAAFQV 580

BLAST of Lag0033759 vs. TAIR 10
Match: AT4G30710.1 (Family of unknown function (DUF566) )

HSP 1 Score: 443.4 bits (1139), Expect = 3.2e-124
Identity = 303/613 (49.43%), Postives = 387/613 (63.13%), Query Frame = 0

Query: 18  TLRQPLVPAEKNNEVITRSSSR-----SKNKSPSPSSLSGSRRCPSPSITR-TVSTPSQL 77
           T R+ L+P++KNN V+     R     S+ +SP+P+    + RCPSPS+TR TVS+ SQ 
Sbjct: 7   TTRRRLLPSDKNNAVVATRRPRTMEVSSRYRSPTPTK---NGRCPSPSVTRPTVSSSSQS 66

Query: 78  V-LKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGR-PEGLWPSTMRSLSV 137
           V  KRA SAERKRPSTPPSP+SP+TPI     D+   S+R+  GR PE LWPSTMRSLSV
Sbjct: 67  VAAKRAVSAERKRPSTPPSPTSPSTPIRDLSIDLPASSRRLSTGRLPESLWPSTMRSLSV 126

Query: 138 SFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSK-QAETQTVARKPTPERKKSPLRSK 197
           SFQSD++S+PVSKKE+PV SS  DRTLRPSSN   K +AET +V+RKPTPERK+SPL+ K
Sbjct: 127 SFQSDSVSVPVSKKERPVSSSSGDRTLRPSSNIAQKHKAETTSVSRKPTPERKRSPLKGK 186

Query: 198 NG-HDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRIPSLNKPLRGN 257
           N   D SEN KPVDG  ++ ++QHRWPSR+G K +SNSL  S+DL DK    +     G 
Sbjct: 187 NNVSDLSENSKPVDGPHSRLIEQHRWPSRIGGKITSNSLNRSLDLGDKASRGIPTSGPGM 246

Query: 258 ELSSTR-ATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQESAANNVVSS 317
             S  R +  + + ++ L +++S       +          KS  +N  + S A  ++S+
Sbjct: 247 GPSLRRMSLPLSSSSRPLHKTSSNTSSYGGLVS------PTKSEDNNIARTSGAQRLLSA 306

Query: 318 -SLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLS--------SVTRGVSPSR------- 377
            SL    + T     +  P  G RP+SPS+T  LS        S +RGVSPSR       
Sbjct: 307 GSLDRATLATAVARLHPLPAPGSRPASPSRTSFLSSSSISRGMSTSRGVSPSRGLSPSRG 366

Query: 378 ------------------------IRPSTTPPRGISSSRIR-PSNSTQSNASTSVLSFIA 437
                                    RPST P RGIS SRIR  + STQS+ +TSVLSFI 
Sbjct: 367 LSPTRGLSPSRGLSPSRGTNTSCFARPSTPPSRGISPSRIRQTTTSTQSSTTTSVLSFIT 426

Query: 438 DFKKGKKAASYIEGAHQLRLLYNRHLQWRCANARAEAVLRNQEVKAERTLLGVWNTTVNL 497
           D KKGKK ASYIE  HQLRLL+NR+LQWR A ARAE+V+  Q + +E TL  VW+    L
Sbjct: 427 DVKKGKK-ASYIEDVHQLRLLHNRYLQWRFAIARAESVMYIQRLTSEETLFNVWHAISEL 486

Query: 498 WDSVIRKRINLQQLKLELKFISIMNDQMSCLNEWAVLERSHNRSLSGVIDDLESSTLRIP 557
            D V R+RI LQQLKLE+K  S++NDQM  L +WA LER H  SL G I DLE++TLR+P
Sbjct: 487 QDHVTRQRIGLQQLKLEIKLNSLLNDQMVSLEDWATLERDHVSSLVGAISDLEANTLRLP 546

Query: 558 VTGGAKADAGSLNGAICSAVEIMQAMGSSICSLLPRVEVIQTLVLELAIVAAQEKAMLDE 579
            TGG KAD  SL  A+ SA+++MQAMGSSI SLL +VE +  +V ELA+V  +E +M  +
Sbjct: 547 ATGGTKADTESLKAAMSSALDVMQAMGSSIWSLLSKVEEMNIMVTELAVVVTKESSMQGK 606

BLAST of Lag0033759 vs. TAIR 10
Match: AT4G30710.2 (Family of unknown function (DUF566) )

HSP 1 Score: 441.4 bits (1134), Expect = 1.2e-123
Identity = 302/613 (49.27%), Postives = 386/613 (62.97%), Query Frame = 0

Query: 18  TLRQPLVPAEKNNEVITRSSSR-----SKNKSPSPSSLSGSRRCPSPSITR-TVSTPSQL 77
           T R+ L+P++KNN V+     R     S+ +SP+P+    + RCPSPS+TR TVS+ SQ 
Sbjct: 7   TTRRRLLPSDKNNAVVATRRPRTMEVSSRYRSPTPTK---NGRCPSPSVTRPTVSSSSQS 66

Query: 78  V-LKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGR-PEGLWPSTMRSLSV 137
           V  KRA SAERKRPSTPPSP+SP+TPI     D+   S+R+  GR PE LWPSTMRSLSV
Sbjct: 67  VAAKRAVSAERKRPSTPPSPTSPSTPIRDLSIDLPASSRRLSTGRLPESLWPSTMRSLSV 126

Query: 138 SFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSK-QAETQTVARKPTPERKKSPLRSK 197
           SFQSD++S+PVSKKE+PV SS  DRTLRPSSN   K +AET +V+RKPTPERK+SPL+ K
Sbjct: 127 SFQSDSVSVPVSKKERPVSSSSGDRTLRPSSNIAQKHKAETTSVSRKPTPERKRSPLKGK 186

Query: 198 NG-HDQSENFKPVDGSRTQFVDQHRWPSRVGAKASSNSLTCSVDLTDKRIPSLNKPLRGN 257
           N   D SEN KPVDG  ++ ++QHRWPSR+G K +SNSL  S+DL DK    +     G 
Sbjct: 187 NNVSDLSENSKPVDGPHSRLIEQHRWPSRIGGKITSNSLNRSLDLGDKASRGIPTSGPGM 246

Query: 258 ELSSTR-ATTVETINKSLQRSTSGVMRLAYVDGRSREEFEAKSAADNSVQESAANNVVSS 317
             S  R +  + + ++ L +++S       +          KS  +N  + S A  ++S+
Sbjct: 247 GPSLRRMSLPLSSSSRPLHKTSSNTSSYGGLVS------PTKSEDNNIARTSGAQRLLSA 306

Query: 318 -SLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLS--------SVTRGVSPSR------- 377
            SL    + T     +  P  G RP+SPS+T  LS        S +RGVSPSR       
Sbjct: 307 GSLDRATLATAVARLHPLPAPGSRPASPSRTSFLSSSSISRGMSTSRGVSPSRGLSPSRG 366

Query: 378 ------------------------IRPSTTPPRGISSSRIR-PSNSTQSNASTSVLSFIA 437
                                    RPST P RGIS SRIR  + STQS+ +TSVLSFI 
Sbjct: 367 LSPTRGLSPSRGLSPSRGTNTSCFARPSTPPSRGISPSRIRQTTTSTQSSTTTSVLSFIT 426

Query: 438 DFKKGKKAASYIEGAHQLRLLYNRHLQWRCANARAEAVLRNQEVKAERTLLGVWNTTVNL 497
           D KKGKK ASYIE  HQLRLL+NR+LQWR A ARAE+V+  Q + +E TL  VW+    L
Sbjct: 427 DVKKGKK-ASYIEDVHQLRLLHNRYLQWRFAIARAESVMYIQRLTSEETLFNVWHAISEL 486

Query: 498 WDSVIRKRINLQQLKLELKFISIMNDQMSCLNEWAVLERSHNRSLSGVIDDLESSTLRIP 557
            D V R+RI LQQLKLE+K  S++NDQM  L +WA LER H  SL G I DLE++TLR+P
Sbjct: 487 QDHVTRQRIGLQQLKLEIKLNSLLNDQMVSLEDWATLERDHVSSLVGAISDLEANTLRLP 546

Query: 558 VTGGAKADAGSLNGAICSAVEIMQAMGSSICSLLPRVEVIQTLVLELAIVAAQEKAMLDE 579
            TGG KAD  SL  A+ SA+++MQAMGSSI SLL +V  +  +V ELA+V  +E +M  +
Sbjct: 547 ATGGTKADTESLKAAMSSALDVMQAMGSSIWSLLSKVRPMNIMVTELAVVVTKESSMQGK 606

BLAST of Lag0033759 vs. TAIR 10
Match: AT2G24070.1 (Family of unknown function (DUF566) )

HSP 1 Score: 381.7 bits (979), Expect = 1.1e-105
Identity = 273/600 (45.50%), Postives = 369/600 (61.50%), Query Frame = 0

Query: 16  AATLRQPLVPAEKNN--EVITRSSS---RSKNKSPSPSSLSGSRRCPSPSITRTV-STPS 75
           A + R PL P+EKNN   V  R+ +    S+ +SP+P+    +RRCPSP +TRT  S+  
Sbjct: 20  ATSPRPPLAPSEKNNVGSVTRRARTMEVSSRYRSPTPTK---TRRCPSPIVTRTAPSSSP 79

Query: 76  QLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGR-PEGLWPSTMRSLS 135
           +  LKRA SAER R      PS+P TP+     D+ + S+R+  GR PE LWPSTMRSLS
Sbjct: 80  ESFLKRAVSAERNR-----GPSTPTTPVSDVLVDLPVSSRRLSTGRLPESLWPSTMRSLS 139

Query: 136 VSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSN--FPSKQAETQTVARKPTPERKKSPLR 195
           VSFQSD++S+PVSKKEKP+++S +DRTLRPSS+     +Q+ET +V RK TPERK+SPL+
Sbjct: 140 VSFQSDSVSVPVSKKEKPLVTSSTDRTLRPSSSNIAHKQQSETTSVTRKQTPERKRSPLK 199

Query: 196 SKN-GHDQSENFKPVDGSRTQFV-DQHRWPSRVGAKASSNSLTCSVDLTDKRIPSLNKPL 255
            KN    QSEN KP+DGS +  +  QHRW  R+           S DL DK +  ++ PL
Sbjct: 200 GKNVSPGQSENSKPMDGSHSMLIPPQHRWSGRIRGNR-------SFDLGDKAVRRVSLPL 259

Query: 256 RGNELSSTRATTVETINKSL--QRSTSGVMRLAYVDGRSREEFEAKSAADNSVQESAANN 315
                           NKS   ++S+S + RL       R E  + + +++S    +  +
Sbjct: 260 S---------------NKSSRHKKSSSDITRLFSCYDNGRLEVSSSTTSEDSSSTESLKH 319

Query: 316 VVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSR----------IR 375
             +SSL  +   +    R  SP+     SS S      S +RGVSP R          +R
Sbjct: 320 FSTSSLPRLHPMSAPGSRTASPSRSSFSSSSSSNSRGMSPSRGVSPMRGLSPVGNRSLVR 379

Query: 376 PSTTPPRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQ 435
            ST P RG+S SRIR   +  S+ +TSVLSFIAD KKGKK A+YIE  HQLRLLYNR+ Q
Sbjct: 380 SSTPPSRGVSPSRIR-QTAQSSSTNTSVLSFIADVKKGKK-ATYIEDVHQLRLLYNRYSQ 439

Query: 436 WRCANARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQ 495
           WR ANARAE V   Q + A+ TL  VW+   +L D V  +RI LQQLKLE+K  SI+NDQ
Sbjct: 440 WRFANARAEGVSYVQSLIAKETLYNVWHAISDLRDLVTTQRICLQQLKLEIKLRSILNDQ 499

Query: 496 MSCLNEWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMG 555
           M CL +WA++ER H  SL+G I DLE++TLR+P+ GG KAD GSL  A+ SA+++MQ+MG
Sbjct: 500 MVCLEDWAMVEREHISSLAGAIGDLEANTLRLPLAGGTKADLGSLKLAMSSALDVMQSMG 559

Query: 556 SSICSLLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQLSTVADKSCLFSESK 593
           SSI SL  ++E +  LV +LA++A  E  +LD+C  LLASTA +++   + K+ L  + +
Sbjct: 560 SSIWSLHSQMEEMNKLVSDLAVIAKTENFLLDKCENLLASTAVMEIEERSLKTHLIQKKQ 587

BLAST of Lag0033759 vs. TAIR 10
Match: AT2G24070.2 (Family of unknown function (DUF566) )

HSP 1 Score: 381.7 bits (979), Expect = 1.1e-105
Identity = 273/600 (45.50%), Postives = 369/600 (61.50%), Query Frame = 0

Query: 16  AATLRQPLVPAEKNN--EVITRSSS---RSKNKSPSPSSLSGSRRCPSPSITRTV-STPS 75
           A + R PL P+EKNN   V  R+ +    S+ +SP+P+    +RRCPSP +TRT  S+  
Sbjct: 20  ATSPRPPLAPSEKNNVGSVTRRARTMEVSSRYRSPTPTK---TRRCPSPIVTRTAPSSSP 79

Query: 76  QLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGR-PEGLWPSTMRSLS 135
           +  LKRA SAER R      PS+P TP+     D+ + S+R+  GR PE LWPSTMRSLS
Sbjct: 80  ESFLKRAVSAERNR-----GPSTPTTPVSDVLVDLPVSSRRLSTGRLPESLWPSTMRSLS 139

Query: 136 VSFQSDTISIPVSKKEKPVLSSPSDRTLRPSSN--FPSKQAETQTVARKPTPERKKSPLR 195
           VSFQSD++S+PVSKKEKP+++S +DRTLRPSS+     +Q+ET +V RK TPERK+SPL+
Sbjct: 140 VSFQSDSVSVPVSKKEKPLVTSSTDRTLRPSSSNIAHKQQSETTSVTRKQTPERKRSPLK 199

Query: 196 SKN-GHDQSENFKPVDGSRTQFV-DQHRWPSRVGAKASSNSLTCSVDLTDKRIPSLNKPL 255
            KN    QSEN KP+DGS +  +  QHRW  R+           S DL DK +  ++ PL
Sbjct: 200 GKNVSPGQSENSKPMDGSHSMLIPPQHRWSGRIRGNR-------SFDLGDKAVRRVSLPL 259

Query: 256 RGNELSSTRATTVETINKSL--QRSTSGVMRLAYVDGRSREEFEAKSAADNSVQESAANN 315
                           NKS   ++S+S + RL       R E  + + +++S    +  +
Sbjct: 260 S---------------NKSSRHKKSSSDITRLFSCYDNGRLEVSSSTTSEDSSSTESLKH 319

Query: 316 VVSSSLAGIKITTNRVVRYDSPTLGPRPSSPSKTPVLSSVTRGVSPSR----------IR 375
             +SSL  +   +    R  SP+     SS S      S +RGVSP R          +R
Sbjct: 320 FSTSSLPRLHPMSAPGSRTASPSRSSFSSSSSSNSRGMSPSRGVSPMRGLSPVGNRSLVR 379

Query: 376 PSTTPPRGISSSRIRPSNSTQSNASTSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQ 435
            ST P RG+S SRIR   +  S+ +TSVLSFIAD KKGKK A+YIE  HQLRLLYNR+ Q
Sbjct: 380 SSTPPSRGVSPSRIR-QTAQSSSTNTSVLSFIADVKKGKK-ATYIEDVHQLRLLYNRYSQ 439

Query: 436 WRCANARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQ 495
           WR ANARAE V   Q + A+ TL  VW+   +L D V  +RI LQQLKLE+K  SI+NDQ
Sbjct: 440 WRFANARAEGVSYVQSLIAKETLYNVWHAISDLRDLVTTQRICLQQLKLEIKLRSILNDQ 499

Query: 496 MSCLNEWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMG 555
           M CL +WA++ER H  SL+G I DLE++TLR+P+ GG KAD GSL  A+ SA+++MQ+MG
Sbjct: 500 MVCLEDWAMVEREHISSLAGAIGDLEANTLRLPLAGGTKADLGSLKLAMSSALDVMQSMG 559

Query: 556 SSICSLLPRVEVIQTLVLELAIVAAQEKAMLDECIALLASTAALQLSTVADKSCLFSESK 593
           SSI SL  ++E +  LV +LA++A  E  +LD+C  LLASTA +++   + K+ L  + +
Sbjct: 560 SSIWSLHSQMEEMNKLVSDLAVIAKTENFLLDKCENLLASTAVMEIEERSLKTHLIQKKQ 587

BLAST of Lag0033759 vs. TAIR 10
Match: AT1G49890.1 (Family of unknown function (DUF566) )

HSP 1 Score: 193.7 bits (491), Expect = 4.4e-49
Identity = 210/660 (31.82%), Postives = 308/660 (46.67%), Query Frame = 0

Query: 13  HTVAATLRQPLVPAEKNNEVITRSSS---RSKNKSPSPSSLSGSRRCPSPSITRTVSTPS 72
           H+V++T           +   + SSS   R+  + PSPS L    R  + S + ++ TPS
Sbjct: 47  HSVSSTTTTTTTTTTTTSSSSSSSSSAILRTSKRYPSPSPLLS--RSTTNSASNSIKTPS 106

Query: 73  QLVLKRAQSAERKRPSTPPSPSSPATPIHGSPADVQLLSKRMIGGRPEGLWPSTMRSLSV 132
            L+ KR+QS +R+RPS                 ++   +K +I         ++ RSLSV
Sbjct: 107 -LLPKRSQSVDRRRPSAVSVTVG---------TEMSAATKMLI---------TSTRSLSV 166

Query: 133 SFQSDTISIPVSKKEKPVLSSPSDRTLRPSSNFPSKQAETQTVARKPTPERKKS-PLRSK 192
           SFQ +  S+P+SKK+        + T  P S+            RK TPER++S P+R  
Sbjct: 167 SFQGEAFSLPISKKK--------ETTSTPVSH------------RKSTPERRRSTPVR-- 226

Query: 193 NGHDQSENFKPVDGSRTQFVDQHRWP--SRVGAKAS------SNSLTCSVDLTDKRIPSL 252
              DQ EN KP        VDQ RWP  SR G   S      S SL C  D        +
Sbjct: 227 ---DQRENSKP--------VDQQRWPGASRRGNSESVVPNSLSRSLDCGSDRGKLGSGFV 286

Query: 253 NKPLRGNEL--SSTRATTVETINKSL--------------QRSTSGVMRLAYVDGRSREE 312
            + +  N +   S R +    ++  L              +R  +G+      D  + + 
Sbjct: 287 GRSMLHNSMIDESPRVSVNGRLSLDLGGRDEYLDIGDDIQRRPNNGLTSSVSCDFTASDT 346

Query: 313 FEAKSAADNSVQE--SAANNVVSSS-------LAGIKI---TTNRVVRYDSPTLGPRPSS 372
               S + N VQE  S  N  +S S       +A  +    T +R+ R   P   P  SS
Sbjct: 347 DSVSSGSTNGVQECGSGVNGEISKSKSLPRNIMASARFWQETNSRLRRLQDPG-SPLSSS 406

Query: 373 PS-----------------------------KTPVLSSVTRGVSPSRIRPSTT--PPRGI 432
           P                               +PV  S  R  SPS++  +TT  P R +
Sbjct: 407 PGLKTSSISSKFGLSKRFSSDAVPLSSPRGMASPVRGSAIRSASPSKLWATTTSSPARAL 466

Query: 433 SS-SRIRPSNSTQSNA-----STSVLSFIADFKKGKKAASYIEGAHQLRLLYNRHLQWRC 492
           SS SR R   S Q NA     + S+LSF AD ++GK     +  AH LRLLYNR LQWR 
Sbjct: 467 SSPSRARNGVSDQMNAYNRNNTPSILSFSADIRRGKIGEDRVMDAHLLRLLYNRDLQWRF 526

Query: 493 ANARAEAVLRNQEVKAERTLLGVWNTTVNLWDSVIRKRINLQQLKLELKFISIMNDQMSC 552
            NARA++ +  Q + AE+ L   W +   L  SV  KRI L  L+ +LK  SI+  QM  
Sbjct: 527 VNARADSTVMVQRLNAEKNLWNAWVSISELRHSVTLKRIKLLLLRQKLKLASILRGQMGF 586

Query: 553 LNEWAVLERSHNRSLSGVIDDLESSTLRIPVTGGAKADAGSLNGAICSAVEIMQAMGSSI 596
           L EW++L+R H+ SLSG  + L++STLR+P+ G    D   L  A+ SAV++MQAM SSI
Sbjct: 587 LEEWSLLDRDHSSSLSGATESLKASTLRLPIVGKTVVDIQDLKHAVSSAVDVMQAMSSSI 646

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879190.19.5e-27288.83AUGMIN subunit 8-like [Benincasa hispida] >XP_038879191.1 AUGMIN subunit 8-like ... [more]
KAA0035102.11.6e-26386.13AUGMIN subunit 8-like [Cucumis melo var. makuwa][more]
TYJ95976.12.8e-26386.13AUGMIN subunit 8-like [Cucumis melo var. makuwa][more]
XP_008443841.12.8e-26386.57PREDICTED: AUGMIN subunit 8-like [Cucumis melo] >XP_008443842.1 PREDICTED: AUGMI... [more]
XP_023515712.12.9e-26085.71AUGMIN subunit 8-like [Cucurbita pepo subsp. pepo] >XP_023515713.1 AUGMIN subuni... [more]
Match NameE-valueIdentityDescription
Q9SUH54.5e-12349.43AUGMIN subunit 8 OS=Arabidopsis thaliana OX=3702 GN=AUG8 PE=1 SV=1[more]
F4INP91.6e-10445.50QWRF motif-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=QWRF4 PE=3 SV... [more]
Q94AI16.2e-4831.82QWRF motif-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=QWRF2 PE=2 SV... [more]
Q8GXD94.0e-4731.53Protein SNOWY COTYLEDON 3 OS=Arabidopsis thaliana OX=3702 GN=SCO3 PE=1 SV=1[more]
F4K4M07.8e-4336.10QWRF motif-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=QWRF9 PE=3 SV... [more]
Match NameE-valueIdentityDescription
A0A5A7SV337.9e-26486.13AUGMIN subunit 8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57... [more]
A0A1S3B9S21.3e-26386.57AUGMIN subunit 8-like OS=Cucumis melo OX=3656 GN=LOC103487338 PE=3 SV=1[more]
A0A5D3BAQ41.3e-26386.13AUGMIN subunit 8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold27... [more]
A0A6J1JLX43.1e-26085.84AUGMIN subunit 8-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485765 PE=... [more]
A0A6J1E6M86.9e-26085.37AUGMIN subunit 8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429893 P... [more]
Match NameE-valueIdentityDescription
AT4G30710.13.2e-12449.43Family of unknown function (DUF566) [more]
AT4G30710.21.2e-12349.27Family of unknown function (DUF566) [more]
AT2G24070.11.1e-10545.50Family of unknown function (DUF566) [more]
AT2G24070.21.1e-10545.50Family of unknown function (DUF566) [more]
AT1G49890.14.4e-4931.82Family of unknown function (DUF566) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007573QWRF familyPFAMPF04484QWRFcoord: 260..564
e-value: 1.4E-84
score: 284.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 322..374
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 174..198
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 152..173
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..74
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..101
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 141..207
NoneNo IPR availablePANTHERPTHR31807AUGMIN FAMILY MEMBERcoord: 1..580
NoneNo IPR availablePANTHERPTHR31807:SF37AUGMIN SUBUNIT 8coord: 1..580

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033759.1Lag0033759.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051225 spindle assembly
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005880 nuclear microtubule
molecular_function GO:0008017 microtubule binding