Lag0033646 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033646
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionvinorine synthase-like
Locationchr3: 847452 .. 849473 (+)
RNA-Seq ExpressionLag0033646
SyntenyLag0033646
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACAAGGAGCTGATAAGGACAACTGCAAATAGGACTGGAGAGTGGACACCGAAGGCGAAACCGGTCAAAAGGTCGGGCCAAAGTCGGAGGGGTCGGGCCTTGGCCCGACCCACCATGTCCATCCCGATTCCACTCGGTGCAGCCGTGGATCGCCTCCGAACGTCTAGAAATCCTAGAGTAGGAACAAGTATTTAAATTCCTCTTCGTGAAGCGATCCTGAACTCTATTCTCTAGTCTCTCTTCTTGTTCTCTTGCTCTCCTCCTTCCATTTTCTGACTTAAGTATCGGAGGCGGTGTGACAAGCACCACACCATTTACGACACGTGGAGGTCAGATGAGTTCTCCTATCCAGATTTTGCCATCAACATATATATTTATATATAAAACTCAACATCAAGATATGAATACATATAACACAGCCAAAAGGGTAGATGCCTATAAAAAATAATTTCATCCACTAATATTAATATTTATCGGAGGTTGCACCTAACTTAATATTTCAAATAATGAAAGGTATTAAAAAAATAGGACCACACACCCTTTGGGAATACTCAAAACAATTTGAATCGAATTTTTTTTTATTAAAAGCATCAATATATATATAAACTTTACGATCATGGAAGCTCCTGCTTGCAAGAACAGAGAAGAAAAGTCTTCTGGTATGTAATTTCAGTTCTTTATTCTGCATCTGATTTCATACTCACAACATTCATGGAGAAAATCAGTGTCGAGATCATTTCAAGGGAGATAATCAAACCCTCATCTCCAACTCCATCATCTCTAATGTATCGAGAACTTTCATTGATCGATCAAATGGCGCCTCCTTCTTACACCCCCGCCATTTTCTTCTATCGCCCTCAGAAATCCGAAAATGTTGATGATCAATCTCTCAAAGTTCGTCGGATTAGGGCTTCTCTCGCACGAACTCTAACTCGCTTCAATCACTTGGCGGGAACCATGACGGAGAGATTCTTTGTCGATTGCAACGACGTTGGAGTCCAATTTGTTGAAGCGAAAGTCGATTGCGAGATTTCGATGCTCTTGGACAATCCAGAAATCGACGTTTTGCATCGATTGCTGCCGCTTGGTTTCACCTCCAATCGTGCGGAATCGAGAAATGGAAGCGAGTTTTTGCTCGCCGTTCAGTTAAGTAGCTTCGTCTGCGGTGGAGTAGCAGTGGCTGTTTGTCTTTCGCACAAGCTCGCCGATGGAGCTTCCGCCGCCACATTTGTTAAAGCGTGGGCAGCCGACGCAAGAGGAGGTAACGAGTCTGTTTTGGAGCCTAATTTTGATGCTGTGAAACTCTTCCCACCTAGAAAAATTCCAGGTTTCAAGCGAGGAATCGAGGCTTCAAAAGAGAAGATCTCGACGAAGAGACTCGTGTTTGCCAAATCCAACATAGCCGCCTTGAAAGAACTCGCTTCTGGTGCTGCTGGTGGCTCCGTCGTAAAACCTCCGTCACGTGTGGTGGCTGTTTCATCCTTCATTTGGCTACGGTTAATTGCACTGGCTCGAACGAGACCAATGAAAGCAAAGCTATTTGGAGCGCTTTATCCGGTGGACTTGCGGGGGAGGATGGATCCGCCGTTGCCGGAAAATTCATTCGGCAATGTTTCCTGGTTTACAATTGCAACATCCCCCGTGGAGATCAATGAAGATTTACCTCTTCTGGTGGCTAAAGTAAGAACCGCCATACAAGAAATCGACTCTGGTTTCGTGAAGAAACTAGAAGATTCTGAAAACTTATTGGATTTGATGAAGCAAGTGGACAAACAGCTCTCCACTGGAGATGTACATCTCTGGAGTTTTACAAGTTGGTGTAGATTTCCCGTTTATGAAGCTGATTTTGGATGGGGAAAGCCCACTTGGGTTTGCAGTCCAAGCAGGCCTTTTCAAAACGTAGTCGTTTTAATGGATACTTCTGATGGCGATGGTGTTGAAGCTTGGGTGAACTTGAAGGAAGAAGACATGGCCATCTTTGAAAAAGACAAAGAGCTTCTTTCCTTTTGCTCAGATTAG

mRNA sequence

ATGTACAAGGAGCTGATAAGGACAACTGCAAATAGGACTGGAGAGTGGACACCGAAGGCGAAACCGGTCAAAAGGTCGGGCCAAAGTCGGAGGGGTCGGGCCTTGGCCCGACCCACCATGTCCATCCCGATTCCACTCGGTGCAGCCGTGGATCGCCTCCGAACGTCTAGAAATCCTAGAGTAGGAACAATTCTTTATTCTGCATCTGATTTCATACTCACAACATTCATGGAGAAAATCAGTGTCGAGATCATTTCAAGGGAGATAATCAAACCCTCATCTCCAACTCCATCATCTCTAATGTATCGAGAACTTTCATTGATCGATCAAATGGCGCCTCCTTCTTACACCCCCGCCATTTTCTTCTATCGCCCTCAGAAATCCGAAAATGTTGATGATCAATCTCTCAAAGTTCGTCGGATTAGGGCTTCTCTCGCACGAACTCTAACTCGCTTCAATCACTTGGCGGGAACCATGACGGAGAGATTCTTTGTCGATTGCAACGACGTTGGAGTCCAATTTGTTGAAGCGAAAGTCGATTGCGAGATTTCGATGCTCTTGGACAATCCAGAAATCGACGTTTTGCATCGATTGCTGCCGCTTGGTTTCACCTCCAATCGTGCGGAATCGAGAAATGGAAGCGAGTTTTTGCTCGCCGTTCAGTTAAGTAGCTTCGTCTGCGGTGGAGTAGCAGTGGCTGTTTGTCTTTCGCACAAGCTCGCCGATGGAGCTTCCGCCGCCACATTTGTTAAAGCGTGGGCAGCCGACGCAAGAGGAGGTAACGAGTCTGTTTTGGAGCCTAATTTTGATGCTGTGAAACTCTTCCCACCTAGAAAAATTCCAGGTTTCAAGCGAGGAATCGAGGCTTCAAAAGAGAAGATCTCGACGAAGAGACTCGTGTTTGCCAAATCCAACATAGCCGCCTTGAAAGAACTCGCTTCTGGTGCTGCTGGTGGCTCCGTCGTAAAACCTCCGTCACGTGTGGTGGCTGTTTCATCCTTCATTTGGCTACGGTTAATTGCACTGGCTCGAACGAGACCAATGAAAGCAAAGCTATTTGGAGCGCTTTATCCGGTGGACTTGCGGGGGAGGATGGATCCGCCGTTGCCGGAAAATTCATTCGGCAATGTTTCCTGGTTTACAATTGCAACATCCCCCGTGGAGATCAATGAAGATTTACCTCTTCTGGTGGCTAAAGTAAGAACCGCCATACAAGAAATCGACTCTGGTTTCGTGAAGAAACTAGAAGATTCTGAAAACTTATTGGATTTGATGAAGCAAGTGGACAAACAGCTCTCCACTGGAGATGTACATCTCTGGAGTTTTACAAGTTGGTGTAGATTTCCCGTTTATGAAGCTGATTTTGGATGGGGAAAGCCCACTTGGGTTTGCAGTCCAAGCAGGCCTTTTCAAAACGTAGTCGTTTTAATGGATACTTCTGATGGCGATGGTGTTGAAGCTTGGGTGAACTTGAAGGAAGAAGACATGGCCATCTTTGAAAAAGACAAAGAGCTTCTTTCCTTTTGCTCAGATTAG

Coding sequence (CDS)

ATGTACAAGGAGCTGATAAGGACAACTGCAAATAGGACTGGAGAGTGGACACCGAAGGCGAAACCGGTCAAAAGGTCGGGCCAAAGTCGGAGGGGTCGGGCCTTGGCCCGACCCACCATGTCCATCCCGATTCCACTCGGTGCAGCCGTGGATCGCCTCCGAACGTCTAGAAATCCTAGAGTAGGAACAATTCTTTATTCTGCATCTGATTTCATACTCACAACATTCATGGAGAAAATCAGTGTCGAGATCATTTCAAGGGAGATAATCAAACCCTCATCTCCAACTCCATCATCTCTAATGTATCGAGAACTTTCATTGATCGATCAAATGGCGCCTCCTTCTTACACCCCCGCCATTTTCTTCTATCGCCCTCAGAAATCCGAAAATGTTGATGATCAATCTCTCAAAGTTCGTCGGATTAGGGCTTCTCTCGCACGAACTCTAACTCGCTTCAATCACTTGGCGGGAACCATGACGGAGAGATTCTTTGTCGATTGCAACGACGTTGGAGTCCAATTTGTTGAAGCGAAAGTCGATTGCGAGATTTCGATGCTCTTGGACAATCCAGAAATCGACGTTTTGCATCGATTGCTGCCGCTTGGTTTCACCTCCAATCGTGCGGAATCGAGAAATGGAAGCGAGTTTTTGCTCGCCGTTCAGTTAAGTAGCTTCGTCTGCGGTGGAGTAGCAGTGGCTGTTTGTCTTTCGCACAAGCTCGCCGATGGAGCTTCCGCCGCCACATTTGTTAAAGCGTGGGCAGCCGACGCAAGAGGAGGTAACGAGTCTGTTTTGGAGCCTAATTTTGATGCTGTGAAACTCTTCCCACCTAGAAAAATTCCAGGTTTCAAGCGAGGAATCGAGGCTTCAAAAGAGAAGATCTCGACGAAGAGACTCGTGTTTGCCAAATCCAACATAGCCGCCTTGAAAGAACTCGCTTCTGGTGCTGCTGGTGGCTCCGTCGTAAAACCTCCGTCACGTGTGGTGGCTGTTTCATCCTTCATTTGGCTACGGTTAATTGCACTGGCTCGAACGAGACCAATGAAAGCAAAGCTATTTGGAGCGCTTTATCCGGTGGACTTGCGGGGGAGGATGGATCCGCCGTTGCCGGAAAATTCATTCGGCAATGTTTCCTGGTTTACAATTGCAACATCCCCCGTGGAGATCAATGAAGATTTACCTCTTCTGGTGGCTAAAGTAAGAACCGCCATACAAGAAATCGACTCTGGTTTCGTGAAGAAACTAGAAGATTCTGAAAACTTATTGGATTTGATGAAGCAAGTGGACAAACAGCTCTCCACTGGAGATGTACATCTCTGGAGTTTTACAAGTTGGTGTAGATTTCCCGTTTATGAAGCTGATTTTGGATGGGGAAAGCCCACTTGGGTTTGCAGTCCAAGCAGGCCTTTTCAAAACGTAGTCGTTTTAATGGATACTTCTGATGGCGATGGTGTTGAAGCTTGGGTGAACTTGAAGGAAGAAGACATGGCCATCTTTGAAAAAGACAAAGAGCTTCTTTCCTTTTGCTCAGATTAG

Protein sequence

MYKELIRTTANRTGEWTPKAKPVKRSGQSRRGRALARPTMSIPIPLGAAVDRLRTSRNPRVGTILYSASDFILTTFMEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSLKVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLHRLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAADARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGNVSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDMAIFEKDKELLSFCSD
Homology
BLAST of Lag0033646 vs. NCBI nr
Match: XP_022135580.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 719.9 bits (1857), Expect = 1.5e-203
Identity = 360/434 (82.95%), Postives = 392/434 (90.32%), Query Frame = 0

Query: 77  MEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 136
           ME I V+IISR+IIKP+SPTP SL+YRELSLIDQMAPPSYTPAIFFYR QKSENV  QS+
Sbjct: 1   MENIGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSV 60

Query: 137 KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLH 196
           KV ++RASLARTLTRFNHLAGTMTERF+VDCNDVGV+F EA+VDCEIS++L+ PEI VLH
Sbjct: 61  KVAQVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLH 120

Query: 197 RLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAAD 256
           RLLPL FT     SRNGSEFLL+VQ SSF CGGVAVAVCLSHKLADGASAA FVK WAA 
Sbjct: 121 RLLPLVFT-----SRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAV 180

Query: 257 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 316
           ARG   SVLEPNFDAVKL+PPRKIPGFKRGIEASKEKI T+RLVF K  IAALKELA+GA
Sbjct: 181 ARGDKHSVLEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELAAGA 240

Query: 317 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 376
            GGSVVKPPSRVVAVS+FIWLRL+ LAR R +KAK+FGALYPVDLR RMDPPLPENSFGN
Sbjct: 241 GGGSVVKPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGN 300

Query: 377 VSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 436
           VSWFT+ATSPVE NEDLPLL  KVR+AIQEIDSGFVKKLEDSENLLDL+KQVDKQ+S+G+
Sbjct: 301 VSWFTMATSPVEANEDLPLLAGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGE 360

Query: 437 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 496
           + LWSFTSWCRFPVYEADFGWGKP+WVCSPSRPF+N VVLMD SDG GVEAWVNLKEEDM
Sbjct: 361 MQLWSFTSWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDM 420

Query: 497 AIFEKDKELLSFCS 511
           AIF  D++LLSFCS
Sbjct: 421 AIFHHDQQLLSFCS 429

BLAST of Lag0033646 vs. NCBI nr
Match: XP_022927112.1 (vinorine synthase-like [Cucurbita moschata])

HSP 1 Score: 711.1 bits (1834), Expect = 7.1e-201
Identity = 356/435 (81.84%), Postives = 388/435 (89.20%), Query Frame = 0

Query: 77  MEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 136
           ME ISVEI+S ++IKPSSPTP SL YRELSLIDQMA P+ TPAIFFYRP   +N+ D+SL
Sbjct: 1   MENISVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPTLTPAIFFYRPSNPQNLADRSL 60

Query: 137 KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLH 196
           KVR+IRASLARTLT F HLAGTM ERF+V CNDVGVQF+EAKVDC+IS LLDNPEI VL 
Sbjct: 61  KVRQIRASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLR 120

Query: 197 RLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAAD 256
           RL+P       AESRNGSEFLL VQ SSF+CGGVAVAVCLSHKLADGAS A FVKAWAA 
Sbjct: 121 RLMP------SAESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASVAAFVKAWAAY 180

Query: 257 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 316
           ARGGNESVLE NFDAVKLFPPRKIPGFKR I+ SKEKIS+KRLVF KS+IAALKELA+GA
Sbjct: 181 ARGGNESVLEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGA 240

Query: 317 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 376
            GGSVV PPSRVVAVS+FIWLRL+ALAR  P+KAKLFGALYPV+LR R DPPLPENSFGN
Sbjct: 241 GGGSVVNPPSRVVAVSAFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGN 300

Query: 377 VSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 436
           VSWFT+AT+PV +NEDLPLLVAKVRTAIQEIDSGFVK LED E LL+LMKQ+DKQ STG+
Sbjct: 301 VSWFTVATTPVAMNEDLPLLVAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGE 360

Query: 437 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 496
           +HLWSFTS CRFP+YEADFGWGKPTWVCSPSRPF+NVVVLMD SDGDGVEAWVNLKEEDM
Sbjct: 361 IHLWSFTSMCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDM 420

Query: 497 AIFEKDKELLSFCSD 512
            IFEKD+ELLSFCS+
Sbjct: 421 TIFEKDEELLSFCSE 429

BLAST of Lag0033646 vs. NCBI nr
Match: KAG7021414.1 (Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 709.1 bits (1829), Expect = 2.7e-200
Identity = 355/435 (81.61%), Postives = 387/435 (88.97%), Query Frame = 0

Query: 76  FMEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQS 135
           FME ISVEI+S ++IKPSSPTP SL YRELSLIDQMA P+ TPAIFFYRP   +N+ D+S
Sbjct: 345 FMENISVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPTLTPAIFFYRPSNPQNLADRS 404

Query: 136 LKVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVL 195
           LKVR+IRASLARTLT F HLAGTM ERF+V CNDVGVQF+EAKVDC+IS LLDNPEI VL
Sbjct: 405 LKVRQIRASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVL 464

Query: 196 HRLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAA 255
            RL+P       AESRNGSEFLL VQ SSF+CGGVAVAVCLSHKLADGAS A FVKAWAA
Sbjct: 465 RRLMP------SAESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASLADFVKAWAA 524

Query: 256 DARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASG 315
            ARGGNESVLE NFDAVKLFPPRKIPGFKR I+ SKEKIS+KRLVF KS+IAALKELA+G
Sbjct: 525 YARGGNESVLEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATG 584

Query: 316 AAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFG 375
           A GGS+V PPSRVVAVS+FIWLRL+ALAR  P+KAKLFGALYPV+LR R DPPLPENSFG
Sbjct: 585 AGGGSLVNPPSRVVAVSTFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFG 644

Query: 376 NVSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTG 435
           NVSWFT+AT+PV +NEDLPLL AKVRTAIQEIDSGFVK LED E LL+LMKQ+DKQ STG
Sbjct: 645 NVSWFTVATTPVAMNEDLPLLAAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTG 704

Query: 436 DVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEED 495
           ++HLWSFTS CRFP+YEADFGWGKPTWVCSPSRPF+NVVVLMD SDGDGVEAWVNLKEED
Sbjct: 705 EIHLWSFTSMCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEED 764

Query: 496 MAIFEKDKELLSFCS 511
           M IFEKD+ELLSFCS
Sbjct: 765 MTIFEKDEELLSFCS 773

BLAST of Lag0033646 vs. NCBI nr
Match: XP_023529997.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 703.0 bits (1813), Expect = 1.9e-198
Identity = 353/434 (81.34%), Postives = 383/434 (88.25%), Query Frame = 0

Query: 77  MEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 136
           ME  SVEI+S ++IKPSSPTP SL YRELSLIDQMA PS TPAIFFYRP   +N+  +SL
Sbjct: 1   MENTSVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPSLTPAIFFYRPSNPQNLAHRSL 60

Query: 137 KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLH 196
           K+R+IRASLARTLT F HLAGTM ERF+V CNDVGVQF+EAKVDC+IS LLDNPEI VL 
Sbjct: 61  KLRQIRASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLR 120

Query: 197 RLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAAD 256
           RL+P       AESRNGSEFLL VQ SSF+CGGVAVAVCLSHKLADGAS A FVKAWAA 
Sbjct: 121 RLMP------SAESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASLAAFVKAWAAY 180

Query: 257 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 316
           ARGGNESVLE NFDAVKLFPPRKIPGFKR I+ SKEKIS+KRLVF KS+IAALKELA+GA
Sbjct: 181 ARGGNESVLEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGA 240

Query: 317 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 376
            GGSV  PPSRVVAVS+FIWLRL+ALAR  P+KAKLFGALYPV+LR R DPPLPENSFGN
Sbjct: 241 GGGSVANPPSRVVAVSAFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGN 300

Query: 377 VSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 436
           VSWFT+AT+PV  NEDLPLL AKVRTAIQEIDSGFVK LED E LL+LMKQ+DKQ STG+
Sbjct: 301 VSWFTVATTPVATNEDLPLLAAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGE 360

Query: 437 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 496
           +HLWSFTS CRFP+YEADFGWGKPTWVCSPSRPF+NVVVLMD SDGDGVEAWVNLKEEDM
Sbjct: 361 IHLWSFTSMCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDM 420

Query: 497 AIFEKDKELLSFCS 511
           AIFEKD+ELLSFCS
Sbjct: 421 AIFEKDEELLSFCS 428

BLAST of Lag0033646 vs. NCBI nr
Match: KAG6587430.1 (Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 703.0 bits (1813), Expect = 1.9e-198
Identity = 352/434 (81.11%), Postives = 384/434 (88.48%), Query Frame = 0

Query: 77  MEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 136
           ME ISVEI+S ++IKPSSPTP SL YRELSLIDQMA P+ TPAIFFYRP   +N+ D+SL
Sbjct: 1   MENISVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPTLTPAIFFYRPSNPQNLADRSL 60

Query: 137 KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLH 196
           KVR+IR SLARTLT F HLAGTM ERF+V CNDVGVQF+EAKVDC+IS LLDNPEI VL 
Sbjct: 61  KVRQIRTSLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLR 120

Query: 197 RLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAAD 256
           RL+P       AESRNGSEFLL VQ SSF+CGGVAVAVCLSHKLADGAS A FVKAWAA 
Sbjct: 121 RLMP------SAESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASLADFVKAWAAY 180

Query: 257 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 316
           ARGGNESVLE NFDAVKLFPPRKIPGFKR I+ SKEKIS+KRLVF KS+IAALKELA+GA
Sbjct: 181 ARGGNESVLEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGA 240

Query: 317 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 376
            GGS+V PPSRVVAVS+FIWLRL+ALAR  P+KAKLFGALYPV+LR R DPPLPENSFGN
Sbjct: 241 GGGSLVNPPSRVVAVSTFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGN 300

Query: 377 VSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 436
           VSWFT+AT+PV +NEDLPLL AKVRTAIQEIDSGFVK LED E LL+LMKQ+DKQ STG+
Sbjct: 301 VSWFTVATTPVAMNEDLPLLAAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGE 360

Query: 437 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 496
           +HLWSFTS CRFP+YEADFGWGKPTWVCSPSRPF+NVVVLMD SDGDGVEAWVNLKEED 
Sbjct: 361 IHLWSFTSMCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDK 420

Query: 497 AIFEKDKELLSFCS 511
            IFEKD+ELLSFCS
Sbjct: 421 TIFEKDEELLSFCS 428

BLAST of Lag0033646 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.3e-69
Identity = 160/425 (37.65%), Postives = 243/425 (57.18%), Query Frame = 0

Query: 82  VEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSLKVRRI 141
           ++I+S E+I+PSSPTP +L   +LS +DQ+    + P I FY  Q   N+ D++ +   +
Sbjct: 5   MQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNL-DRAQRSENL 64

Query: 142 RASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLHRLLPL 201
           + SL+  LT+F  LAG +     VDCND GV F+EA+V  ++S  + N  ID L++ LP 
Sbjct: 65  KRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQYLPF 124

Query: 202 GFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAADARGGN 261
                  ES    +  LAV++S F CGG A+ VC+SHK+AD  S ATF+ +W A  +   
Sbjct: 125 QPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQEET 184

Query: 262 ESVLEPNFD-AVKLFPPRK---IPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGAA 321
           + +++PNFD     FPP +    P F        E I  KR VF K  + ALK  A  A+
Sbjct: 185 D-IVQPNFDLGSHHFPPMESIPAPEF-----LPDENIVMKRFVFDKEKLEALK--AQLAS 244

Query: 322 GGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGNV 381
             + VK  SRV  V + IW + I + R +        A   V+LR RM+PP P+++ GN+
Sbjct: 245 SATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAAQAVNLRSRMNPPFPQSAMGNI 304

Query: 382 SWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGDV 441
           +  T+A +  E ++D   LV  ++T++ +ID   VK+L+     LD   +  +       
Sbjct: 305 A--TMAYAVAEEDKDFSDLVGPLKTSLAKIDDEHVKELQKGVTYLDYEAEPQE------- 364

Query: 442 HLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDMA 501
            L+SF+SWCR   Y+ DFGWGKP  VC+ + P +N+V LMDT + DG+EAW+++ E++M+
Sbjct: 365 -LFSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWISMAEDEMS 410

Query: 502 IFEKD 503
           +   D
Sbjct: 425 MLSSD 410

BLAST of Lag0033646 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 256.5 bits (654), Expect = 6.2e-67
Identity = 168/429 (39.16%), Postives = 233/429 (54.31%), Query Frame = 0

Query: 82  VEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSLKVRRI 141
           +E +S E+I PSSPTP SL   ++S +DQ+    + P I FY      N+D      + +
Sbjct: 5   MEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTS-QHL 64

Query: 142 RASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDN-PEIDVLHRLLP 201
           + SL++ LT F  LAG +     VDCND GV FVEA+V  ++S  + N  E++ L + LP
Sbjct: 65  KQSLSKVLTHFYPLAGRINVNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLDQYLP 124

Query: 202 -LGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAADARG 261
              +   + E     +  LAV++S F CGG A+ V LSHK+AD  S ATF+ AW A  RG
Sbjct: 125 SAAYPGGKIEV--NEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATCRG 184

Query: 262 GNESVLEPNFD-AVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGAAG 321
             E VL PNFD A + FPP  +           E +  KR VF K  I AL+  AS A  
Sbjct: 185 ETEIVL-PNFDLAARHFPP--VDNTPSPELVPDENVVMKRFVFDKEKIGALRAQASSA-- 244

Query: 322 GSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGNVS 381
            S  K  SRV  V ++IW  +I + R +      F  +  V+LR RM+PPLP  + GN++
Sbjct: 245 -SEEKNFSRVQLVVAYIWKHVIDVTRAKYGAKNKFVVVQAVNLRSRMNPPLPHYAMGNIA 304

Query: 382 WFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGDVH 441
               A    E ++D P L+  +RT+++        K ED  N  +L+K +          
Sbjct: 305 TLLFAAVDAEWDKDFPDLIGPLRTSLE--------KTEDDHN-HELLKGMTCLYELEPQE 364

Query: 442 LWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDMAI 501
           L SFTSWCR   Y+ DFGWGKP   C+ + P +N  +LMDT  GDGVEAW+ + E++MA+
Sbjct: 365 LLSFTSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGDGVEAWLPMAEDEMAM 413

Query: 502 FEKDKELLS 508
                ELLS
Sbjct: 425 L--PVELLS 413

BLAST of Lag0033646 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.7e-54
Identity = 147/431 (34.11%), Postives = 225/431 (52.20%), Query Frame = 0

Query: 82  VEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSLKV-RR 141
           VEI+S+  IKPSSPTP SL    LS IDQ      T   FFY    + N   +S K+   
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFY----TYNPSHESSKIGDL 65

Query: 142 IRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLHRLLP 201
           ++ SL++TL  +   AG + E  ++DCND GV+FVE ++   ++ +L   +      +LP
Sbjct: 66  LKNSLSKTLVSYYQFAGRLIENDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDLVLP 125

Query: 202 LGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAADARGG 261
               +   +S      LL VQLS F CGG+A+    SHK++DG S   F+K WA+     
Sbjct: 126 TRIIALHEDS------LLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASSTSLS 185

Query: 262 NESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGAAGGS 321
                 P   A  +FPP            S ++   KR VF+   I  LK  A       
Sbjct: 186 TFHKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIEYG--- 245

Query: 322 VVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGNVSWF 381
            ++ PSRV  V++F+       A     + K +  +  V+LR  +   LP+NS GN+  F
Sbjct: 246 -IQKPSRVEVVTAFL---CQCAANCDLPRKKPYAIISAVNLRPYL--ALPQNSIGNIFSF 305

Query: 382 TIATSPVEINEDLPLLVAKVRTAIQEIDSGFV--KKLEDSENLLDLMKQVDKQLSTGDVH 441
               +   ++     L++K+R   Q++    +  +KL     + +L K +D QL+   + 
Sbjct: 306 YFCINDEGMDNQFSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCLD-QLNISSLD 365

Query: 442 LWSFTSWCRFPVYEADFGWGKPTWVCSPSRP-FQNVVVLMDTSDGDGVEAWVNLKEEDMA 501
            +  +SWCRFPVY+ DFGWGKP  V SP +P  +++++LMD+ +GDG+EA + ++E+ MA
Sbjct: 366 TYFCSSWCRFPVYDIDFGWGKPILV-SPFQPHVKDLILLMDSPEGDGIEALITMEEKKMA 415

Query: 502 IFEKDKELLSF 509
            FEK++EL SF
Sbjct: 426 AFEKNEELRSF 415

BLAST of Lag0033646 vs. ExPASy Swiss-Prot
Match: Q58VT1 (Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.1e-52
Identity = 145/447 (32.44%), Postives = 242/447 (54.14%), Query Frame = 0

Query: 82  VEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFY-----RPQKSENVDDQSL 141
           V I  +  IKPSS TPS+L + +LS IDQ     Y P  FFY     R + S+N D+ S 
Sbjct: 10  VSICDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 142 KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNP-----E 201
               ++ SL++TL  +   AG + +   VDCND+G +F+  ++ C +S +LD+P     E
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAE 129

Query: 202 IDVLHRLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVK 261
             VL + LP    +N  E  N    LL VQ+S F CGG+A++VC SHK+ DG S   F+ 
Sbjct: 130 SIVLPKDLP---WANNCEGGN----LLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLN 189

Query: 262 AWAADARGGNESVL--EPNFDAVKLFPPRKIPG-FKRGIEASKEKISTKRLVFAKSNIAA 321
            W++  R    + L   P F    +F  +K        I +   +   KRL+F    + A
Sbjct: 190 DWSSVTRDHTTTTLVPSPRFVGDSVFSTKKYGSLITPQILSDLNECVQKRLIFPTDKLDA 249

Query: 322 LKELASGAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPP 381
           L+   +  +G   VK P+R   VS+ ++ +    A +  + +KL   ++ +++R  + P 
Sbjct: 250 LRAKVAEESG---VKNPTRAEVVSALLF-KCATKASSSMLPSKL---VHFLNIRTMIKPR 309

Query: 382 LPENSFGNV-SWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVK-KLEDSENLLDLMK 441
           LP N+ GN+ S F+I  + ++ + +LP LV  +R   +E++  + K ++E +E +L++++
Sbjct: 310 LPRNAIGNLSSIFSIEATNMQ-DMELPTLVRNLR---KEVEVAYKKDQVEQNELILEVVE 369

Query: 442 QVDK-----QLSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSD 501
            + +     +   G  ++++ ++ C++P Y  DFGWG+P  VC  + P +N   L D   
Sbjct: 370 SMREGKLPFENMDGYENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKA 429

Query: 502 GDGVEAWVNLKEEDMAIFEKDKELLSF 509
           G GVEA V L ++ M+ FE+++ELL F
Sbjct: 430 GQGVEARVMLHKQQMSEFERNEELLEF 438

BLAST of Lag0033646 vs. ExPASy Swiss-Prot
Match: D2Y3X2 (Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.5e-52
Identity = 144/447 (32.21%), Postives = 243/447 (54.36%), Query Frame = 0

Query: 82  VEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFY-----RPQKSENVDDQSL 141
           V + ++  IKPSS TPS+L + +LS IDQ     Y P  FFY     R + S+N D+ S 
Sbjct: 10  VSVCNKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 142 KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNP-----E 201
               ++ SL++TL  +   AG + +   VDCND+G +F+  ++ C +S +LD+P     E
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAE 129

Query: 202 IDVLHRLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVK 261
             VL + LP    +N  E  N    LL VQ+S F CGG+A++VC SHK+ DG S   F+ 
Sbjct: 130 SIVLPKDLP---WANNCEGGN----LLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLN 189

Query: 262 AWAADARGGNESVL--EPNFDAVKLFPPRKIPG-FKRGIEASKEKISTKRLVFAKSNIAA 321
            W++  R    + L   P F    +F  +K        I +   +   KRL+F    + A
Sbjct: 190 DWSSVTRDRTTTTLVPSPRFVGDSVFSTQKYGSLITPQILSDLNQCVQKRLIFPTDKLDA 249

Query: 322 LKELASGAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPP 381
           L+   +  +G   VK P+R   VS+ ++ +    A +  + +KL   ++ +++R  + P 
Sbjct: 250 LRAKVAEESG---VKNPTRAEVVSALLF-KCATKASSSMLPSKL---VHFLNIRTMIKPR 309

Query: 382 LPENSFGNV-SWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVK-KLEDSENLLDLMK 441
           LP N+ GN+ S F+I  + ++ + +LP LV  +R   +E++  + K ++E +E +L++++
Sbjct: 310 LPRNAIGNLSSIFSIEATNMQ-DMELPTLVRNLR---KEVEVAYKKDQVEQNELILEVVE 369

Query: 442 QVDK-----QLSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSD 501
            + +     +   G  ++++ ++ C++P Y  DFGWG+P  VC  + P +N   L D   
Sbjct: 370 SMREGKLPFENMDGYKNVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKA 429

Query: 502 GDGVEAWVNLKEEDMAIFEKDKELLSF 509
           G GVEA V L ++ M+ FE+++ELL F
Sbjct: 430 GQGVEARVMLHKQQMSEFERNEELLEF 438

BLAST of Lag0033646 vs. ExPASy TrEMBL
Match: A0A6J1C1E9 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111007495 PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 7.4e-204
Identity = 360/434 (82.95%), Postives = 392/434 (90.32%), Query Frame = 0

Query: 77  MEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 136
           ME I V+IISR+IIKP+SPTP SL+YRELSLIDQMAPPSYTPAIFFYR QKSENV  QS+
Sbjct: 1   MENIGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSV 60

Query: 137 KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLH 196
           KV ++RASLARTLTRFNHLAGTMTERF+VDCNDVGV+F EA+VDCEIS++L+ PEI VLH
Sbjct: 61  KVAQVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLH 120

Query: 197 RLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAAD 256
           RLLPL FT     SRNGSEFLL+VQ SSF CGGVAVAVCLSHKLADGASAA FVK WAA 
Sbjct: 121 RLLPLVFT-----SRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAV 180

Query: 257 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 316
           ARG   SVLEPNFDAVKL+PPRKIPGFKRGIEASKEKI T+RLVF K  IAALKELA+GA
Sbjct: 181 ARGDKHSVLEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELAAGA 240

Query: 317 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 376
            GGSVVKPPSRVVAVS+FIWLRL+ LAR R +KAK+FGALYPVDLR RMDPPLPENSFGN
Sbjct: 241 GGGSVVKPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGN 300

Query: 377 VSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 436
           VSWFT+ATSPVE NEDLPLL  KVR+AIQEIDSGFVKKLEDSENLLDL+KQVDKQ+S+G+
Sbjct: 301 VSWFTMATSPVEANEDLPLLAGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGE 360

Query: 437 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 496
           + LWSFTSWCRFPVYEADFGWGKP+WVCSPSRPF+N VVLMD SDG GVEAWVNLKEEDM
Sbjct: 361 MQLWSFTSWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDM 420

Query: 497 AIFEKDKELLSFCS 511
           AIF  D++LLSFCS
Sbjct: 421 AIFHHDQQLLSFCS 429

BLAST of Lag0033646 vs. ExPASy TrEMBL
Match: A0A6J1EG83 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111434048 PE=3 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 3.4e-201
Identity = 356/435 (81.84%), Postives = 388/435 (89.20%), Query Frame = 0

Query: 77  MEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 136
           ME ISVEI+S ++IKPSSPTP SL YRELSLIDQMA P+ TPAIFFYRP   +N+ D+SL
Sbjct: 1   MENISVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPTLTPAIFFYRPSNPQNLADRSL 60

Query: 137 KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLH 196
           KVR+IRASLARTLT F HLAGTM ERF+V CNDVGVQF+EAKVDC+IS LLDNPEI VL 
Sbjct: 61  KVRQIRASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLR 120

Query: 197 RLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAAD 256
           RL+P       AESRNGSEFLL VQ SSF+CGGVAVAVCLSHKLADGAS A FVKAWAA 
Sbjct: 121 RLMP------SAESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASVAAFVKAWAAY 180

Query: 257 ARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 316
           ARGGNESVLE NFDAVKLFPPRKIPGFKR I+ SKEKIS+KRLVF KS+IAALKELA+GA
Sbjct: 181 ARGGNESVLEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGA 240

Query: 317 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 376
            GGSVV PPSRVVAVS+FIWLRL+ALAR  P+KAKLFGALYPV+LR R DPPLPENSFGN
Sbjct: 241 GGGSVVNPPSRVVAVSAFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGN 300

Query: 377 VSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGD 436
           VSWFT+AT+PV +NEDLPLLVAKVRTAIQEIDSGFVK LED E LL+LMKQ+DKQ STG+
Sbjct: 301 VSWFTVATTPVAMNEDLPLLVAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGE 360

Query: 437 VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDM 496
           +HLWSFTS CRFP+YEADFGWGKPTWVCSPSRPF+NVVVLMD SDGDGVEAWVNLKEEDM
Sbjct: 361 IHLWSFTSMCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDM 420

Query: 497 AIFEKDKELLSFCSD 512
            IFEKD+ELLSFCS+
Sbjct: 421 TIFEKDEELLSFCSE 429

BLAST of Lag0033646 vs. ExPASy TrEMBL
Match: A0A5D3BIG7 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00830 PE=3 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 3.1e-194
Identity = 353/436 (80.96%), Postives = 386/436 (88.53%), Query Frame = 0

Query: 77  MEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 136
           ME   VEII+ +IIKPSS TP+   Y +LSLIDQMAPPSY PAIFFY P  S+N+     
Sbjct: 1   MEDFGVEIIAMDIIKPSSSTPN---YFQLSLIDQMAPPSYMPAIFFYLPPNSQNI----- 60

Query: 137 KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLH 196
            V +IR SL+RTLTRF  LAGTM ERF+VDCND GV+F EAKVDCEIS+LL NPEI +LH
Sbjct: 61  -VHQIRDSLSRTLTRFYPLAGTMPERFYVDCNDSGVEFSEAKVDCEISVLLKNPEIRILH 120

Query: 197 RLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAAD 256
           RLLPL FTS+ A+S+    FLLAVQL+ F+CGGVAVAVCLSHKLADGASAA FVKAWAA+
Sbjct: 121 RLLPLVFTSHSADSK----FLLAVQLTRFICGGVAVAVCLSHKLADGASAAGFVKAWAAE 180

Query: 257 ARGG-NESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASG 316
           ARGG N S+LEPNFDAVKLFP RKIPGFKRGI ASKEKISTKR VFAKS+I  LK LA G
Sbjct: 181 ARGGSNNSILEPNFDAVKLFPCRKIPGFKRGIVASKEKISTKRFVFAKSDIDVLKSLAVG 240

Query: 317 AAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFG 376
             GGS+ KPPSRVVAVS+FIWLRL+ALARTRP+KAK+FGALYPVDLR RMDPPLP+NSFG
Sbjct: 241 TFGGSISKPPSRVVAVSAFIWLRLMALARTRPVKAKVFGALYPVDLRARMDPPLPKNSFG 300

Query: 377 NVSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTG 436
           NVSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSE+LL+LMKQVDKQLS+G
Sbjct: 301 NVSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSEHLLELMKQVDKQLSSG 360

Query: 437 DVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEED 496
           +VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPF+N VVLMD+SDGDGVEAWVNLKEED
Sbjct: 361 EVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFRNSVVLMDSSDGDGVEAWVNLKEED 420

Query: 497 MAIFEKDKELLSFCSD 512
           MAIFEKD+ELLSFCSD
Sbjct: 421 MAIFEKDEELLSFCSD 423

BLAST of Lag0033646 vs. ExPASy TrEMBL
Match: A0A5A7USW8 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G00800 PE=3 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 3.1e-194
Identity = 353/436 (80.96%), Postives = 386/436 (88.53%), Query Frame = 0

Query: 77  MEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 136
           ME   VEII+ +IIKPSS TP+   Y +LSLIDQMAPPSY PAIFFY P  S+N+     
Sbjct: 1   MEDFGVEIIAMDIIKPSSSTPN---YFQLSLIDQMAPPSYMPAIFFYLPPNSQNI----- 60

Query: 137 KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLH 196
            V +IR SL+RTLTRF  LAGTM ERF+VDCND GV+F EAKVDCEIS+LL NPEI +LH
Sbjct: 61  -VHQIRDSLSRTLTRFYPLAGTMPERFYVDCNDSGVEFSEAKVDCEISVLLKNPEIRILH 120

Query: 197 RLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAAD 256
           RLLPL FTS+ A+S+    FLLAVQL+ F+CGGVAVAVCLSHKLADGASAA FVKAWAA+
Sbjct: 121 RLLPLVFTSHSADSK----FLLAVQLTRFICGGVAVAVCLSHKLADGASAAGFVKAWAAE 180

Query: 257 ARGG-NESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASG 316
           ARGG N S+LEPNFDAVKLFP RKIPGFKRGI ASKEKISTKR VFAKS+I  LK LA G
Sbjct: 181 ARGGCNNSILEPNFDAVKLFPCRKIPGFKRGIVASKEKISTKRFVFAKSDIDVLKSLAVG 240

Query: 317 AAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFG 376
             GGS+ KPPSRVVAVS+FIWLRL+ALARTRP+KAK+FGALYPVDLR RMDPPLP+NSFG
Sbjct: 241 TFGGSISKPPSRVVAVSAFIWLRLMALARTRPVKAKVFGALYPVDLRARMDPPLPKNSFG 300

Query: 377 NVSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTG 436
           NVSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSE+LL+LMKQVDKQLS+G
Sbjct: 301 NVSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSEHLLELMKQVDKQLSSG 360

Query: 437 DVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEED 496
           +VHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPF+N VVLMD+SDGDGVEAWVNLKEED
Sbjct: 361 EVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFRNSVVLMDSSDGDGVEAWVNLKEED 420

Query: 497 MAIFEKDKELLSFCSD 512
           MAIFEKD+ELLSFCSD
Sbjct: 421 MAIFEKDEELLSFCSD 423

BLAST of Lag0033646 vs. ExPASy TrEMBL
Match: A0A1S3CLJ4 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103502350 PE=3 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 5.9e-169
Identity = 302/354 (85.31%), Postives = 327/354 (92.37%), Query Frame = 0

Query: 159 MTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLHRLLPLGFTSNRAESRNGSEFLL 218
           M ERF+VDCND GV+F EAKVDCEIS+LL NPEI +LHRLLPL FTS+ A+S+    FLL
Sbjct: 1   MPERFYVDCNDSGVEFSEAKVDCEISVLLKNPEIRILHRLLPLVFTSHSADSK----FLL 60

Query: 219 AVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAADARGG-NESVLEPNFDAVKLFPP 278
           AVQL+ F+CGGVAVAVCLSHKLADGASAA FVKAWAA+ARGG N S+LEPNFDAVKLFP 
Sbjct: 61  AVQLTRFICGGVAVAVCLSHKLADGASAAGFVKAWAAEARGGSNNSILEPNFDAVKLFPC 120

Query: 279 RKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELASGAAGGSVVKPPSRVVAVSSFIWL 338
           RKIPGFKRGI ASKEKISTKR VFAKS+I  LK LA G  GGS+ KPPSRVVAVS+FIWL
Sbjct: 121 RKIPGFKRGIVASKEKISTKRFVFAKSDIDVLKSLAVGTFGGSISKPPSRVVAVSAFIWL 180

Query: 339 RLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGNVSWFTIATSPVEINEDLPLLV 398
           RL+ALARTRP+KAK+FGALYPVDLR RMDPPLP+NSFGNVSWFTIATSPVEINEDLPLLV
Sbjct: 181 RLMALARTRPVKAKVFGALYPVDLRARMDPPLPKNSFGNVSWFTIATSPVEINEDLPLLV 240

Query: 399 AKVRTAIQEIDSGFVKKLEDSENLLDLMKQVDKQLSTGDVHLWSFTSWCRFPVYEADFGW 458
           AKVRTAIQEIDSGFVKKLEDSE+LL+LMKQVDKQLS+G+VHLWSFTSWCRFPVYEADFGW
Sbjct: 241 AKVRTAIQEIDSGFVKKLEDSEHLLELMKQVDKQLSSGEVHLWSFTSWCRFPVYEADFGW 300

Query: 459 GKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNLKEEDMAIFEKDKELLSFCSD 512
           GKPTWVCSPSRPF+N VVLMD+SDGDGVEAWVNLKEEDMAIFEKD+ELLSFCSD
Sbjct: 301 GKPTWVCSPSRPFRNSVVLMDSSDGDGVEAWVNLKEEDMAIFEKDEELLSFCSD 350

BLAST of Lag0033646 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.7e-83
Identity = 174/440 (39.55%), Postives = 260/440 (59.09%), Query Frame = 0

Query: 80  ISVEIISREIIKPSSPTPSSL----MYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQS 139
           + + + S+E++KPS   P +L     +  LS +DQ+APP + P +FFY      N+ D+ 
Sbjct: 3   LEITVTSQELVKPS---PRNLNHPPCHHHLSFLDQLAPPIFMPFLFFY--HNKTNLSDKE 62

Query: 140 LKVRRIRASLARTLTRFNHLAGTM-TERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDV 199
            +   I++SL+  L  +  LAG +      V CNDVGV FVEAK DC +S +L+NP  + 
Sbjct: 63  -RSDHIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNE 122

Query: 200 LHRLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWA 259
           L++L P  F          S+  L VQL+ F CGG+A+ + LSHKL D  S   FV +WA
Sbjct: 123 LNKLHPFEF-------HEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWA 182

Query: 260 ADARGGNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKISTKRLVFAKSNIAALKELAS 319
           A ARG  + ++ P+FD  K+FPP  I         +KE I T+R VF +S++ +L+E  S
Sbjct: 183 AFARGQTDEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFS 242

Query: 320 GAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSF 379
               G+     +RV  +S FIW R +A         K++  ++PV+LR + DP +P+N F
Sbjct: 243 ----GNKKIRATRVEVLSVFIWSRFMASTNHDDKTGKIYTLIHPVNLRRQADPDIPDNMF 302

Query: 380 GNVSWFTIATSPVEIN---EDLPLLVAKVRTAIQEIDSGFVKKL-EDSENLLDLMKQVDK 439
           GN+  F++    + IN   E+   LV ++R  I++ID+ +VKKL ED+   L+ + +   
Sbjct: 303 GNIMRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQAS 362

Query: 440 QLSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVN 499
               G++  +SFTS C+FPVYEADFGWGKP WV S    ++N+V  +DT +GDG+EAW+N
Sbjct: 363 GFVNGEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWIN 422

Query: 500 LKEEDMAIFEKDKELLSFCS 511
           L + DM+ FE D+ELL + S
Sbjct: 423 LDQNDMSRFEADEELLRYVS 425

BLAST of Lag0033646 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 290.0 bits (741), Expect = 3.6e-78
Identity = 172/439 (39.18%), Postives = 248/439 (56.49%), Query Frame = 0

Query: 82  VEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSLKVRRI 141
           V+++SR+IIKPSSPTP+ L   +LSL++Q+ P  + P +FFY    S    +Q   ++ +
Sbjct: 3   VDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQ---LQML 62

Query: 142 RASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEIDVLHRLLPL 201
           + SL+ TLT F  LAG +     +DCND G  F+EA+V+  +S LL  P  D L +L+P 
Sbjct: 63  KKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLIPT 122

Query: 202 GFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAA-DARGG 261
              S    +R     LL  Q S F CG +++ VC+SHKLAD  S   F+K+WAA  +RG 
Sbjct: 123 SVDSIETRTR-----LLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGS 182

Query: 262 NESVLEPNFDAVKLFPPRKI----PGFKRGIEASKEKISTKRLVFAKSNIAALKELASGA 321
            +++  P FD VK+FPP       P      E    +  +KR +F  S+I AL+  AS  
Sbjct: 183 IKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKASSF 242

Query: 322 AGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPENSFGN 381
                V  P+RV AVS+ IW   +   RT    +K       V LR R+ PP  +NS GN
Sbjct: 243 E----VNQPTRVEAVSALIWKSAMKATRTVSGTSKPSILANSVSLRSRVSPPFTKNSIGN 302

Query: 382 VSWFTIATSPVEINE-DLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLM----KQVDKQ 441
           +  +  A +   IN+  L  LV+K+R A Q      + KL  + N  +++    K+    
Sbjct: 303 LVSYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGDM 362

Query: 442 LSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRPFQNVVVLMDTSDGDGVEAWVNL 501
           +++GD   + F+S CRF +YE DFGWGKP WV  PS   +N+V L+DT +  G+EAWVNL
Sbjct: 363 IASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEAWVNL 422

Query: 502 KEEDMAIFEKDKELLSFCS 511
            E++M +FE+D+ELL F S
Sbjct: 423 NEQEMNLFEQDRELLQFAS 429

BLAST of Lag0033646 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 250.4 bits (638), Expect = 3.2e-66
Identity = 162/445 (36.40%), Postives = 254/445 (57.08%), Query Frame = 0

Query: 77  MEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYR--PQK--SENVD 136
           MEK +VEI+SREI+KPSSPTP       LSL+D ++ P YT A+ FY   PQ     + +
Sbjct: 1   MEK-NVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTE 60

Query: 137 DQSLKVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKVDCEISMLLDNPEI 196
           + SLK+++   SL++TL  F  LAG +    FV+CND G  F+EA+VD  +S  L  P  
Sbjct: 61  ETSLKLKK---SLSKTLPIFYPLAGRIIGS-FVECNDEGAVFIEARVDHLLSEFLKCPVP 120

Query: 197 DVLHRLLPLGFTSNRAESRNGSEF-LLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVK 256
           + L  L+P+      A+SR    + +L +Q + F CGG+ + +C+SHK+ D  S A F++
Sbjct: 121 ESLELLIPV-----EAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIR 180

Query: 257 AWAADARGGNESVLEPNFDAVKLFPP--RKIPG--FKRGIEASKEKISTKRLVFAKSNIA 316
            WA  +RG   +++ P+F A ++FP    ++P     R  E  +    TKR VF  S I 
Sbjct: 181 GWAESSRGLGITLI-PSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIK 240

Query: 317 ALKELASGAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDP 376
            L+  AS     ++VK P+RV AV++  W  +  ++R   +  +       V+LRG++D 
Sbjct: 241 KLRAKAS----RNLVKNPTRVEAVTALFWRCVTKVSRLSSLTPRTSVLQILVNLRGKVD- 300

Query: 377 PLPENSFGNVSWFTIATSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDLMKQ 436
            L EN+ GN+    I  +     E +  +V ++R A +       +  + S  + +L+++
Sbjct: 301 SLCENTIGNMLSLMILKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSSSRIFELLEE 360

Query: 437 VDKQLSTG-DVHLWSFTSWCRFPVYEADFGWGKPTWVCS-PSRPFQNVVVLMDTSDGDGV 496
           + K    G ++ LW   SWC+  +Y+ADFGWGKP WV    +  F+N+++L+DT DG+G+
Sbjct: 361 IGKVYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGI 420

Query: 497 EAWVNLKEEDMAIFEKDKELLSFCS 511
           EAW+ L EE M++FE D+ELL   S
Sbjct: 421 EAWITLTEEQMSLFECDQELLESAS 429

BLAST of Lag0033646 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 243.8 bits (621), Expect = 3.0e-64
Identity = 160/447 (35.79%), Postives = 245/447 (54.81%), Query Frame = 0

Query: 80  ISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSLKVR 139
           + VE IS+EIIKPSSPTP++L   +LS+ D + PP YT A  FY      ++  Q     
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFY---TKNDLISQEHTSH 65

Query: 140 RIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKV-DCEISMLLDNPEIDVLHRL 199
           +++ SL+ TLT+F  LAG +T    VDC D G  FV+A+V +C ++  L  P+ D L +L
Sbjct: 66  KLKTSLSETLTKFYPLAGRIT-GVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQL 125

Query: 200 LPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAADAR 259
           LPL    N   +   +  LL V+ + F CGG+A+ +C++HK+AD AS +TF+++WAA AR
Sbjct: 126 LPLDVVDNPYVAA-ATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATAR 185

Query: 260 GGNESVL--EPNFDAVKLFPPR----KIPGFKRGIEASKEKISTKRLVFAKSNIAALKEL 319
           G N++     P F     +PP     K+P  +   +A K    TKR VF  S +   ++L
Sbjct: 186 GENDAAAMESPVFAGANFYPPANEAFKLPADE---QAGKRSSITKRFVFEASKV---EDL 245

Query: 320 ASGAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPEN 379
            + AA    V  P+RV +V++ IW   +A ++T     K+   L   +LR ++   L E+
Sbjct: 246 RTKAASEETVDQPTRVESVTALIWKCFVASSKTTTCDHKVLVQL--ANLRSKIPSLLQES 305

Query: 380 SFGNVSWFTIATS-----PVEINE---DLPLLVAKVRTAIQEIDSGFVKKLEDSENLLDL 439
           S GN+ + ++  S      V+I E   DL     ++ T I +              L +L
Sbjct: 306 SIGNLMFSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLANL 365

Query: 440 MKQVDKQLSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRP-FQNVVVLMDTSDGD 499
           M     +LS      ++ +SWC+ P+YEA FGW  P WV     P   N+ +L+D+ DG 
Sbjct: 366 MLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAMLIDSKDGQ 425

Query: 500 GVEAWVNLKEEDMAIFEKDKELLSFCS 511
           G+EA+V L EE+M+ FE++ ELL+F +
Sbjct: 426 GIEAFVTLPEENMSSFEQNPELLAFAT 439

BLAST of Lag0033646 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 241.9 bits (616), Expect = 1.1e-63
Identity = 156/451 (34.59%), Postives = 243/451 (53.88%), Query Frame = 0

Query: 77  MEKISVEIISREIIKPSSPTPSSLMYRELSLIDQMAPPSYTPAIFFYRPQKSENVDDQSL 136
           ME + VE I++EIIKPSS TP+ L   +LS+ D + PP YT A  FY      + +  S 
Sbjct: 1   METMKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPEQSS- 60

Query: 137 KVRRIRASLARTLTRFNHLAGTMTERFFVDCNDVGVQFVEAKV-DCEISMLLDNPEIDVL 196
              +++ SLA TLT+F  LAG + +   +DCND G  FV+A+V +  +S  L +P+   L
Sbjct: 61  --HKLKTSLAETLTKFYPLAGRI-KGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTL 120

Query: 197 HRLLPLGFTSNRAESRNGSEFLLAVQLSSFVCGGVAVAVCLSHKLADGASAATFVKAWAA 256
            +LLPL    +  E+      LL V+ + F CGG+A+ +C++HK+AD  S +TF+++WAA
Sbjct: 121 QQLLPLDVIDDPYEAATTWPLLL-VKATYFPCGGMAIGLCITHKIADATSISTFIQSWAA 180

Query: 257 DARG-GNESVLEPNFDAVKLFPPRKIPGFKRGIEASKEKIS--TKRLVFAKSNIAALKEL 316
            ARG   + V +P F A   +PP     FK  ++ +  KIS  TKR VF+ S    L+EL
Sbjct: 181 MARGEAGDIVADPVFAAANFYPPAN-ELFKFPVDENANKISQITKRFVFSASK---LEEL 240

Query: 317 ASGAAGGSVVKPPSRVVAVSSFIWLRLIALARTRPMKAKLFGALYPVDLRGRMDPPLPEN 376
            + AA    V  P RV +V++ +W   +A A +      +   + P +LR ++   L E+
Sbjct: 241 RTKAASEDFVGRPKRVESVTALLWKAFVAAASSNTNACDMKVLIQPSNLRPKIPSLLAES 300

Query: 377 SFGNVSWFTIATSPVEINEDLPLLVAKVRTAIQEI------------DSGFVKKLEDSEN 436
             GN+ + ++     +  E       K+  A++++            + G          
Sbjct: 301 LIGNIMFSSVVLGISQQEE------IKIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGSK 360

Query: 437 LLDLMKQVDKQLSTGDVHLWSFTSWCRFPVYEADFGWGKPTWVCSPSRP-FQNVVVLMDT 496
           L +LM     +LS      ++ +SWC+ P+YEA FGWG P W+     P  +NV +L+D+
Sbjct: 361 LANLMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDS 420

Query: 497 SDGDGVEAWVNLKEEDMAIFEKDKELLSFCS 511
            DG G+EA+V L EE+M   E++ ELL+F S
Sbjct: 421 KDGQGIEAFVTLPEENMLSLEQNTELLAFAS 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135580.11.5e-20382.95vinorine synthase-like [Momordica charantia][more]
XP_022927112.17.1e-20181.84vinorine synthase-like [Cucurbita moschata][more]
KAG7021414.12.7e-20081.61Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023529997.11.9e-19881.34vinorine synthase-like [Cucurbita pepo subsp. pepo][more]
KAG6587430.11.9e-19881.11Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
A0A2P1GIW71.3e-6937.65Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Q70PR76.2e-6739.16Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2[more]
A0A2K8FQU52.7e-5434.11Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT... [more]
Q58VT11.1e-5232.44Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1[more]
D2Y3X21.5e-5232.21Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C1E97.4e-20482.95vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111007495 PE=3 SV=1[more]
A0A6J1EG833.4e-20181.84vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111434048 PE=3 SV=1[more]
A0A5D3BIG73.1e-19480.96Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... [more]
A0A5A7USW83.1e-19480.96Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2... [more]
A0A1S3CLJ45.9e-16985.31vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103502350 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G24430.11.7e-8339.55HXXXD-type acyl-transferase family protein [more]
AT3G26040.13.6e-7839.18HXXXD-type acyl-transferase family protein [more]
AT1G24420.13.2e-6636.40HXXXD-type acyl-transferase family protein [more]
AT4G15390.13.0e-6435.79HXXXD-type acyl-transferase family protein [more]
AT3G30280.11.1e-6334.59HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 291..507
e-value: 1.5E-63
score: 215.7
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 79..281
e-value: 6.0E-60
score: 204.2
IPR003480TransferasePFAMPF02458Transferasecoord: 81..505
e-value: 7.5E-86
score: 288.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..34
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 79..510
NoneNo IPR availablePANTHERPTHR31623:SF25VINORINE SYNTHASE-LIKEcoord: 79..510
NoneNo IPR availableSUPERFAMILY52777CoA-dependent acyltransferasescoord: 339..450

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033646.1Lag0033646.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups