Lag0033545 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033545
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr3: 198248 .. 203300 (+)
RNA-Seq ExpressionLag0033545
SyntenyLag0033545
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTGTTCATCATCGGACACAAATTGCTTTATTAGGAAACAGGTTCGTTCACCATTGAATATTTCTTTCATTTTTACCATTGAAGTTCTAAATTTCTGTGTATCTGTGCCATTTTTGTGAAAAATTGATTTTTTCAGCTGGTATAGTTTAGTTTTGGAATATAATTGAAGCCAACGTTTCATAAAATTTCTTATTTGCAAATGTTGACTCAATTTTGTGAGGCATGATGTTTTTATTCTGTCCATTTTATTTGTTTATATTTCCTGTAATATCGTTTAATTCATCATTCAAGAAGTTGATGATATGTAGTTGATTATATCTTTCCGTTGATCAACACAAAACTCTTGGTGCTCAGTTCAATGCCAAGCATTTGTTGATTCCTATTGTGGTTTGCATCTTTGAAATTTTATTATTACTGCTGATTACTTTAGGCATCTCGCTGATGTGCAAGTAAACTATTAGGGGATTTACCTTGTCTATCAATTTGATAAATTGTCTTTATTCTAATAAGAGTATGATTGTGTTGTTATCCAGACTCCATGTGGCCACAATTTCTGCTTAAAATGTTTCCAGAAGTGGATTGGACAAGGAAAAAACACTTGTGCAAAGTGCCGCTGTGTGATCCCTTCCAAAATGGCTAGCCAGCCCCGTATTAATTCCACCCTTGTAGTGGCCATTCGTATGGCTAAAATGTCAAAATCTATTGGTTCTGGAGGACCTCAGAAGGTTTATCATTTTGTCCACAATCAAAATAGACCAGATAAAGCCTATACCACTGACCGGGCACAGAAAAAAGGGAAAGCTAATGCTGCAAGTGGAAAAATATTTGTTACAATTCCATCCGATCATTTTGGTCCAATTCCTGCTGAAAATGACCCTGAGAGAAACCAGGGTGTTCTGGTTGGTGAATGCTGGGAGGATAGGCTAGAATGTCGGCAATGGGGTGCTCACTTTCCACATGTTGCAGGTATAGCAGGCCAGTCAAATAATGGTGCTCAATCAGTGGTTCTTTCTGGTGGCTACCAGGATGATGAAGATCATGGGGAGTGGTTCCTCTACACTGGAAGGTTATTTTTTTTTCTTTCTGTACTCTATGCAGTCTGTTCTTTCCCTGTCCTTGTTGGTTACGCAATACGTCCTTGTCTTTTTCCTGTTCTGCAAACTTTAAACGAGCCAGGCTTTCAGATGGCCTGCTCTTGTGTTAAATAAACTTATGATAAGATATTTTAGTTCATTTTTCATTGGTTAGTACCATTCCCCATCTTGTCTCTTCTATTTTCCTGTTTGTTTTTAGCTTCTTAAGATTTTGACCTGTTTGGTACAGAGTAAATTTGGTGGTATAATATTAACTTATTGCAATATTTTTTTATTCTCTTTTACATTTTTCGGTCACTATGCATGATAAACCATTGTGTTTGATGATTTTGCCTGGATTTTAATTGCAGTGGGGGTCGAGACCTTAGTGGGAACAAGCGTACGAGCAAGGATCAGTCTTTTGACCAGAAGTTTGAAAAATCAAACAAAGCGCTACAAGTTAGTTGCTTGAAAGGTTATCCTGTTAGGGTTGTTAGGTAGGTGCAGATTGATTTATTTGATTTTTGATTTATGTGGAACTGGAAAAGCACATGGTTTAAATTAGTCTCATTGATTTTATGTCCTGTGTAACGTGAGTAAACTTCTGATTTTAAAACTAAAGAAATGCCGTTAAAGTCGTTAAACTACAACTGTTTTGGTGGAGCATGTTCAACATGTTTTTCCATGCTGGGTAGAGGTATGGGAAAGCCAGAAAACTCACTCATTCATCTGTCGCTTGTCAAAATTGCGCCTGCTTTTTCCCAGACTTGAAGTTTCTTTGTGCCTTTTTCAGTCGTGATCGCATAGCAATTGGTGAAATAGCTTAGGAAAATTAGTGTTACTCTTGGCTTCTTGATGTTGTATGTTGGAGTAGATCTTTAATCCAAGTAAGGGTGTGAAGTTTATTAATGCGGTATGTTAATCTTCTTGATAACAGATCTCACAAAGAGAAGCGTTCCTCGTATGCTCCAGAGAAGGGTTTGCGGTATGATGGAATTTATAGGATTGAGAAGTGTTGGCGTAAAGTGGGAATTCAGGTATTCAAGCTATTCCTCCTTTCCTAAACTACCAGCTGCTGAACAAATTATTGATTAATATGTGTATCCTGAAATTAGGGCTTTAAGGTATGTCGGTATTTATTTGTTAGATGCGACAATGATCCTGCTCCATGGACAAGGTTTGTGTTTCCCAATAATGTAATTGCTTTTGTGTTATCAAATGCTAGTTGCAGTCTCTGGCTTTGCCTCCAAAGCTTTTTCCTTTCATTAAACAAGTTATTTAACTACAGCGATGAACATGGAGATCGACCTAGACCCTTACCTGCAATTCCAGAGTTAAAAAAGGCCACAGATATTACGGAGAGGAAGGAAGGTCCATCATGGGACTTTGATGTGAGTATACCCTAGTTATATTTCACTTGTGAGATCTCCTTCTCCTCCAATATTTCACTTTTATTAGATTATAATTATAATTAAATCGAATCAGTTCCTTGTCTGAATATTAGTTTTTCTAACGATACAAGTTCTTTGAAAAGATAGCGAGCTTTCTTTGAGTTTTGGTTGTTAGTAACCAAGGGCAGGTAGGCACTAGATAGCCTCTTTGTTGTAATACTATCTAATTTGAAGAACCCTTCAATTTTTATTTTTATTTTTTATTTTTTATTTTTTTTGAAAAACCCTTCAAAAACTTTATAATGTTTGGTTATACAATCAATGTTTTAAAAGGCTAAAACATTACCCTTGAGGCTTTTCCTCTTGAAGAGGCGAGGTGCAAGCCTTGAGGCGGTGTGATGCATAAGCCTCAATTTTAATTAAAAACATTACGTAAAAACCTTTATATTTTTAAAGCCTAAGTGGTAAAAATAGTAATATTTTAAATTGTAACAACTCAAACCTTTATATTTTTAAAGAATAGGGAAATTAATACTATTCTTCTAACCATTATATGAGGTGTAGAGACTTTAAGCCTCAGCGCCTTTTTATAAAGGCATAAGCCTCTCCTGTGAGAGGCGTAAGCCTCCTATGGTTCAAAGAAGGCGTAAGTCTCGAGGCTAAAAGTTAGAGCTTCACCTCGCGGTGAGCCTCAAGGCATAAGTCTCAATAATCTTTTAAAACATTGTATATAATCAAAAGTAATTCTTAAAATGTTTTCAGACTTGCGCTAAATGAATTAGCTGTTGCAAATTTGTGTGCATATCCTGTTTTAAAGACTTTTCATATACTCATATTTTCAGTCTGTTCGATAATTTCTTTTTCATTCTTTTTTCTGTTTCTGAAAAATAAGTTTTGAAATACTAGTCAAAAAAGCTAAGATAGTTTCTAAAAGTTGTTTATGTTCTAGCATTTAGCTTACATTTTGGTTATATTTTTAAATATCTAGAGCAATTACGAAATATTGTTTTACTAAAGGAACTTACCTGTATGGAAACAAAGGGAAAAGAAAAACAAAAAATCTAATAAACGTCACATACATTGTTAGAGTTTTATAAGAATTTATGAAATCTTGTGTATGTAATCTATTTCAAGGATTTTTCCCCTTAAACAAAATTGATTTTGACCTTAAGATTAAAACAATGATTCTAATAAGTGAAAGGCAGTGCCAACAAAATGAAGTTGTTCTATTCTGAACGAGTTTTAATTTTTGACTAGTCTAAAATATTCATAAATTTCCGTGAACCAGTTTTAGTATAAACTAAAGTTGTTTCTACTAGCATTCATCTACTTTCAAAGATGGAAGTTGTTTAGAAATTTTTTTTGCCACCAAAACATAATTTTTATTTTTAAGAGTAAAATAAAATTGTATTTAAAAGTAGATTCCCCTAATTGGCCTTCAATTTTTCTGACACTTCCAATTTTGTGAAATGCAGTTTTACTTGTATATTATTTCCTTGCGTTTGGGCAAATACTGTATTCAAGTGATACAATCTAAAGCTTGTGAAATTGAAAGGCGAAATTGTGTAGATTTGCCTGGTTTAGATTTGGTTGGTTCTAGTGTGGCTAGTCTCTTGCGGAATAAAATTAAAGATTGTAGATACCGTGGTGTTTGGTTCACTCAATTCTATTACAATTTATCTAAGCTAATAAATGATAAATGAAGCGTCTTGTGGTAAATTTATGAACCCTCTGCAAGTTCTTTTTCAGGAGAAAGATAGTCGGTGGAAGTGGAACAAAACCCCACCAACGAGCAAGAAACCAGTTGAAACCGAAGATTCTGCTTCTGGGAAGAGGTCAAGGAGAAGAATTAGGCAATCGCATAATATGAGTGTCCGAGAAAGGTTACTGAAAGGTTGTCTTATTATATTGAGTAAAGTCGCTAAAAAATGAAGGCGGTTTGGTGTTGAACATGACGAGGCATTACTAACGACACGTTTTTTTTAACATATTCCATTTGCTGCATGTAACTGTGTATACAGAGTTTAGCTGCCTTACTTGTCGGGAGGTAATGAGTCTGCCAATCACTACGCCTTGTGCCCATAACTTTTGCAAGACATGCTTGGAAGGTGCTTTTGTGGGCAAGACATTCTTGAGAGAGAGAAGCAGTGGTGGACGGGCCCTTAGATCCCAAAAGAATGTCATGAGTTGTCCTTGTTGCCCGACAGATATATCTGATTTTCTACAGAATCTCCAGGTGTGTGTCGCGAAAGATTCTTTTTGTTTTAACATAAAGAAAATTGTTCCTCTCTCACTTGGCTAGTTGCTACTATCAATAGGTAAATAGAGAGTTATTGGATGTGATCGAGTCGTTGAAAAGTAAGATTGAAGAAGGGGATGCATCATCTGAGAAGCTCTGTGAAGAAGAGATAGAAGGGGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGGAAAAGATGGAAGCAGCAGTCCTGTGGAAACAAGGAAGCGAGCAAAGGCGGTGGTTAATGGTGGGGAGGAAAATGCTGTGGGCGGGAATGGAGATGATTCACCCTCAAGTCCCCTCCACGTGGATGATTTGGAATGA

mRNA sequence

ATGTTGTGTTCATCATCGGACACAAATTGCTTTATTAGGAAACAGACTCCATGTGGCCACAATTTCTGCTTAAAATGTTTCCAGAAGTGGATTGGACAAGGAAAAAACACTTGTGCAAAGTGCCGCTGTGTGATCCCTTCCAAAATGGCTAGCCAGCCCCGTATTAATTCCACCCTTGTAGTGGCCATTCGTATGGCTAAAATGTCAAAATCTATTGGTTCTGGAGGACCTCAGAAGGTTTATCATTTTGTCCACAATCAAAATAGACCAGATAAAGCCTATACCACTGACCGGGCACAGAAAAAAGGGAAAGCTAATGCTGCAAGTGGAAAAATATTTGTTACAATTCCATCCGATCATTTTGGTCCAATTCCTGCTGAAAATGACCCTGAGAGAAACCAGGGTGTTCTGGTTGGTGAATGCTGGGAGGATAGGCTAGAATGTCGGCAATGGGGTGCTCACTTTCCACATGTTGCAGGTATAGCAGGCCAGTCAAATAATGGTGCTCAATCAGTGGTTCTTTCTGGTGGCTACCAGGATGATGAAGATCATGGGGAGTGGTTCCTCTACACTGGAAGTGGGGGTCGAGACCTTAGTGGGAACAAGCGTACGAGCAAGGATCAGTCTTTTGACCAGAAGTTTGAAAAATCAAACAAAGCGCTACAAGTTAGTTGCTTGAAAGGTTATCCTGTTAGGGTTGTTAGATCTCACAAAGAGAAGCGTTCCTCGTATGCTCCAGAGAAGGGTTTGCGGTATGATGGAATTTATAGGATTGAGAAGTGTTGGCGTAAAGTGGGAATTCAGGGCTTTAAGGTATGTCGGTATTTATTTGTTAGATGCGACAATGATCCTGCTCCATGGACAAGCGATGAACATGGAGATCGACCTAGACCCTTACCTGCAATTCCAGAGTTAAAAAAGGCCACAGATATTACGGAGAGGAAGGAAGGTCCATCATGGGACTTTGATGAGAAAGATAGTCGGTGGAAGTGGAACAAAACCCCACCAACGAGCAAGAAACCAGTTGAAACCGAAGATTCTGCTTCTGGGAAGAGGTCAAGGAGAAGAATTAGGCAATCGCATAATATGAGTGTCCGAGAAAGGTTACTGAAAGAGTTTAGCTGCCTTACTTGTCGGGAGGTAATGAGTCTGCCAATCACTACGCCTTGTGCCCATAACTTTTGCAAGACATGCTTGGAAGGTGCTTTTGTGGGCAAGACATTCTTGAGAGAGAGAAGCAGTGGTGGACGGGCCCTTAGATCCCAAAAGAATGTCATGAGTTGTCCTTGTTGCCCGACAGATATATCTGATTTTCTACAGAATCTCCAGGTAAATAGAGAGTTATTGGATGTGATCGAGTCGTTGAAAAGTAAGATTGAAGAAGGGGATGCATCATCTGAGAAGCTCTGTGAAGAAGAGATAGAAGGGGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGGAAAAGATGGAAGCAGCAGTCCTGTGGAAACAAGGAAGCGAGCAAAGGCGGTGGTTAATGGTGGGGAGGAAAATGCTGTGGGCGGGAATGGAGATGATTCACCCTCAAGTCCCCTCCACGTGGATGATTTGGAATGA

Coding sequence (CDS)

ATGTTGTGTTCATCATCGGACACAAATTGCTTTATTAGGAAACAGACTCCATGTGGCCACAATTTCTGCTTAAAATGTTTCCAGAAGTGGATTGGACAAGGAAAAAACACTTGTGCAAAGTGCCGCTGTGTGATCCCTTCCAAAATGGCTAGCCAGCCCCGTATTAATTCCACCCTTGTAGTGGCCATTCGTATGGCTAAAATGTCAAAATCTATTGGTTCTGGAGGACCTCAGAAGGTTTATCATTTTGTCCACAATCAAAATAGACCAGATAAAGCCTATACCACTGACCGGGCACAGAAAAAAGGGAAAGCTAATGCTGCAAGTGGAAAAATATTTGTTACAATTCCATCCGATCATTTTGGTCCAATTCCTGCTGAAAATGACCCTGAGAGAAACCAGGGTGTTCTGGTTGGTGAATGCTGGGAGGATAGGCTAGAATGTCGGCAATGGGGTGCTCACTTTCCACATGTTGCAGGTATAGCAGGCCAGTCAAATAATGGTGCTCAATCAGTGGTTCTTTCTGGTGGCTACCAGGATGATGAAGATCATGGGGAGTGGTTCCTCTACACTGGAAGTGGGGGTCGAGACCTTAGTGGGAACAAGCGTACGAGCAAGGATCAGTCTTTTGACCAGAAGTTTGAAAAATCAAACAAAGCGCTACAAGTTAGTTGCTTGAAAGGTTATCCTGTTAGGGTTGTTAGATCTCACAAAGAGAAGCGTTCCTCGTATGCTCCAGAGAAGGGTTTGCGGTATGATGGAATTTATAGGATTGAGAAGTGTTGGCGTAAAGTGGGAATTCAGGGCTTTAAGGTATGTCGGTATTTATTTGTTAGATGCGACAATGATCCTGCTCCATGGACAAGCGATGAACATGGAGATCGACCTAGACCCTTACCTGCAATTCCAGAGTTAAAAAAGGCCACAGATATTACGGAGAGGAAGGAAGGTCCATCATGGGACTTTGATGAGAAAGATAGTCGGTGGAAGTGGAACAAAACCCCACCAACGAGCAAGAAACCAGTTGAAACCGAAGATTCTGCTTCTGGGAAGAGGTCAAGGAGAAGAATTAGGCAATCGCATAATATGAGTGTCCGAGAAAGGTTACTGAAAGAGTTTAGCTGCCTTACTTGTCGGGAGGTAATGAGTCTGCCAATCACTACGCCTTGTGCCCATAACTTTTGCAAGACATGCTTGGAAGGTGCTTTTGTGGGCAAGACATTCTTGAGAGAGAGAAGCAGTGGTGGACGGGCCCTTAGATCCCAAAAGAATGTCATGAGTTGTCCTTGTTGCCCGACAGATATATCTGATTTTCTACAGAATCTCCAGGTAAATAGAGAGTTATTGGATGTGATCGAGTCGTTGAAAAGTAAGATTGAAGAAGGGGATGCATCATCTGAGAAGCTCTGTGAAGAAGAGATAGAAGGGGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGGAAAAGATGGAAGCAGCAGTCCTGTGGAAACAAGGAAGCGAGCAAAGGCGGTGGTTAATGGTGGGGAGGAAAATGCTGTGGGCGGGAATGGAGATGATTCACCCTCAAGTCCCCTCCACGTGGATGATTTGGAATGA

Protein sequence

MLCSSSDTNCFIRKQTPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGGPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGVLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGRDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIYRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERKEGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSCLTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGKDGSSSPVETRKRAKAVVNGGEENAVGGNGDDSPSSPLHVDDLE
Homology
BLAST of Lag0033545 vs. NCBI nr
Match: XP_022134816.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like isoform X1 [Momordica charantia])

HSP 1 Score: 975.7 bits (2521), Expect = 1.6e-280
Identity = 482/518 (93.05%), Postives = 495/518 (95.56%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSG 75
           TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSI SG
Sbjct: 152 TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIVSG 211

Query: 76  GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 135
           GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG
Sbjct: 212 GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 271

Query: 136 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 195
           VLVGECWEDRLECRQWGAHFPHVAGIAGQS+NGAQSVVLSGGYQDDEDHGEWFLYTGSGG
Sbjct: 272 VLVGECWEDRLECRQWGAHFPHVAGIAGQSSNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 331

Query: 196 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 255
           RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI
Sbjct: 332 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 391

Query: 256 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 315
           YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPR LPAIPELKKATDITERK
Sbjct: 392 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRALPAIPELKKATDITERK 451

Query: 316 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 375
           EGPSWDFDEKDSRWKW+K PPTSKKPVETEDSASGKRSRR++RQS NMSVRE+LLKEFSC
Sbjct: 452 EGPSWDFDEKDSRWKWSKPPPTSKKPVETEDSASGKRSRRKVRQSQNMSVREKLLKEFSC 511

Query: 376 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 435
           L CREVM+LPITTPCAHNFCK+CLEGAFVGKTFLRERSSGGRALRSQKNVM+CPCCPTDI
Sbjct: 512 LICREVMTLPITTPCAHNFCKSCLEGAFVGKTFLRERSSGGRALRSQKNVMTCPCCPTDI 571

Query: 436 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGKDGSSSP- 495
           SDFLQNLQVNRELLDVIESLKSKIEEGD +SEKLCEE  +GEEE +E         SSP 
Sbjct: 572 SDFLQNLQVNRELLDVIESLKSKIEEGD-TSEKLCEEGTDGEEEGKE--------CSSPV 631

Query: 496 -VETRKRAKAVVNGGEENAVGGNGDDSPSSPLHVDDLE 532
            VETRKRAK V +GGEEN VGG+GDDSPSSPLHVDDLE
Sbjct: 632 GVETRKRAKVVADGGEENGVGGDGDDSPSSPLHVDDLE 660

BLAST of Lag0033545 vs. NCBI nr
Match: XP_023531327.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 950.3 bits (2455), Expect = 7.2e-273
Identity = 474/522 (90.80%), Postives = 490/522 (93.87%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSG 75
           TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SG
Sbjct: 155 TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISG 214

Query: 76  GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 135
           G QKVYH VHN NRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQG
Sbjct: 215 GSQKVYHVVHNLNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQG 274

Query: 136 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 195
           VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG
Sbjct: 275 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 334

Query: 196 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 255
           RDLSGNKRTSKDQSFDQKFEKSNKALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGI
Sbjct: 335 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGI 394

Query: 256 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 315
           YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK
Sbjct: 395 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 454

Query: 316 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 375
           + PSWDFDEKDSRWKW+K PPTSKK VETEDS SGK+SRRR+RQSHNM+VRERLLKEFSC
Sbjct: 455 KDPSWDFDEKDSRWKWSKPPPTSKKSVETEDSTSGKKSRRRMRQSHNMNVRERLLKEFSC 514

Query: 376 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 435
           L CREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS+KNVM+CPCCPTDI
Sbjct: 515 LICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRSRKNVMACPCCPTDI 574

Query: 436 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE-EEEEEEEEEGKDG---- 495
           SDFLQNLQVNRELLDVIE+LKSKIEE DA SEK CE+EIEGE E E+E+EEEGKDG    
Sbjct: 575 SDFLQNLQVNRELLDVIETLKSKIEEADA-SEKPCEDEIEGEGEGEQEQEEEGKDGSNGN 634

Query: 496 -SSSPVETRKRAKAVVNGGEENAVGGNGDDSPSSPLHVDDLE 532
            SS PVETRKRAK VVNGGEEN       DSPSSP+HVDDLE
Sbjct: 635 ESSRPVETRKRAKVVVNGGEEN-------DSPSSPVHVDDLE 668

BLAST of Lag0033545 vs. NCBI nr
Match: XP_022965844.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita maxima])

HSP 1 Score: 949.9 bits (2454), Expect = 9.4e-273
Identity = 475/522 (91.00%), Postives = 489/522 (93.68%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSG 75
           TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SG
Sbjct: 155 TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISG 214

Query: 76  GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 135
           G QKVYH VHN NRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQG
Sbjct: 215 GSQKVYHVVHNLNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQG 274

Query: 136 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 195
           VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG
Sbjct: 275 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 334

Query: 196 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 255
           RDLSGNKRTSKDQSFDQKFEKSNKALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGI
Sbjct: 335 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGI 394

Query: 256 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 315
           YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK
Sbjct: 395 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 454

Query: 316 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 375
           + PSWDFDEKDSRWKW+K PPTSKK VETEDS SGK+SRRR+RQSHNM+VRERLLKEFSC
Sbjct: 455 KDPSWDFDEKDSRWKWSKPPPTSKKSVETEDSTSGKKSRRRMRQSHNMNVRERLLKEFSC 514

Query: 376 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 435
           L CREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS+KNVM+CPCCPTDI
Sbjct: 515 LICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRSRKNVMACPCCPTDI 574

Query: 436 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE-EEEEEEEEEGKDG---- 495
           SDFLQNLQVNRELLDVIESLKSKIEE DA SEK CE+EIEGE E E E+EEEGKDG    
Sbjct: 575 SDFLQNLQVNRELLDVIESLKSKIEEADA-SEKPCEDEIEGEGEGEGEQEEEGKDGSNGN 634

Query: 496 -SSSPVETRKRAKAVVNGGEENAVGGNGDDSPSSPLHVDDLE 532
            SS PVETRKRAK VVNGGEEN       DSPSSP+HVDDLE
Sbjct: 635 ESSRPVETRKRAKVVVNGGEEN-------DSPSSPVHVDDLE 668

BLAST of Lag0033545 vs. NCBI nr
Match: KAG6587385.1 (E3 ubiquitin-protein ligase ORTHRUS 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021368.1 E3 ubiquitin-protein ligase ORTHRUS 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 948.3 bits (2450), Expect = 2.7e-272
Identity = 473/522 (90.61%), Postives = 489/522 (93.68%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSG 75
           TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SG
Sbjct: 155 TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISG 214

Query: 76  GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 135
           G QKVYH VHN NRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQG
Sbjct: 215 GSQKVYHVVHNLNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQG 274

Query: 136 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 195
           VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG
Sbjct: 275 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 334

Query: 196 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 255
           RDLSGNKRTSKDQSFDQKFEKSNKALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGI
Sbjct: 335 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGI 394

Query: 256 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 315
           YRIEKCWRKVGIQGFKVCRYLFVRC+NDPAPWTSDEHGDRPRPLPAIPELKKATDITERK
Sbjct: 395 YRIEKCWRKVGIQGFKVCRYLFVRCENDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 454

Query: 316 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 375
           + PSWDFDE DSRWKW+K PPTSKK VE EDS SGK+SRRR+RQSHNM+VRERLLKEFSC
Sbjct: 455 KDPSWDFDENDSRWKWSKPPPTSKKSVEPEDSTSGKKSRRRMRQSHNMNVRERLLKEFSC 514

Query: 376 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 435
           L CREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS+KNVM+CPCCPTDI
Sbjct: 515 LICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRSRKNVMACPCCPTDI 574

Query: 436 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE-EEEEEEEEEGKDGS--- 495
           SDFLQNLQVNRELLDVIESLKSKIEE DA SEK CE+EIEGE EEE+E+EEEGKDGS   
Sbjct: 575 SDFLQNLQVNRELLDVIESLKSKIEEADA-SEKPCEDEIEGEGEEEQEQEEEGKDGSNGN 634

Query: 496 --SSPVETRKRAKAVVNGGEENAVGGNGDDSPSSPLHVDDLE 532
             S PVETRKRAK VVNGGEEN       DSPSSP+HVDDLE
Sbjct: 635 EISRPVETRKRAKVVVNGGEEN-------DSPSSPVHVDDLE 668

BLAST of Lag0033545 vs. NCBI nr
Match: XP_022933744.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita moschata])

HSP 1 Score: 947.2 bits (2447), Expect = 6.1e-272
Identity = 473/526 (89.92%), Postives = 490/526 (93.16%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSG 75
           TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SG
Sbjct: 155 TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISG 214

Query: 76  GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 135
           G QKVYH VHN NRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQG
Sbjct: 215 GSQKVYHVVHNLNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQG 274

Query: 136 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 195
           VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG
Sbjct: 275 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 334

Query: 196 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 255
           RDLSGNKRTSKDQSFDQKFEKSNKALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGI
Sbjct: 335 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGI 394

Query: 256 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 315
           YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK
Sbjct: 395 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 454

Query: 316 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 375
           + PSWDFDEKD+RWKW+K PPTSKK VETEDS SGK+SRRR+RQSHNM+VRERLLKEFSC
Sbjct: 455 KDPSWDFDEKDNRWKWSKPPPTSKKSVETEDSTSGKKSRRRMRQSHNMNVRERLLKEFSC 514

Query: 376 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 435
           L CREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS+KNVM+CPCCPTDI
Sbjct: 515 LICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRSRKNVMACPCCPTDI 574

Query: 436 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEG-----EEEEEEEEEEGKDG 495
           SDFLQNLQVNRELLDVIESLKSKIEE DA SEK CE+EIEG     EE+E+E+EEEGKDG
Sbjct: 575 SDFLQNLQVNRELLDVIESLKSKIEEADA-SEKPCEDEIEGEGEGEEEQEQEQEEEGKDG 634

Query: 496 -----SSSPVETRKRAKAVVNGGEENAVGGNGDDSPSSPLHVDDLE 532
                SS PVETRKRAK VVNGGEEN        SPSSP+HVDDLE
Sbjct: 635 SNGNESSRPVETRKRAKVVVNGGEENY-------SPSSPVHVDDLE 672

BLAST of Lag0033545 vs. ExPASy Swiss-Prot
Match: Q8VYZ0 (E3 ubiquitin-protein ligase ORTHRUS 2 OS=Arabidopsis thaliana OX=3702 GN=ORTH2 PE=1 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 2.6e-201
Identity = 340/473 (71.88%), Postives = 392/473 (82.88%), Query Frame = 0

Query: 17  PCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGG 76
           PCGHN CLKCF+KW+GQGK TC KCR +IP KMA  PRINS+LV AIR+AK+SKS  +  
Sbjct: 160 PCGHNACLKCFEKWMGQGKRTCGKCRSIIPEKMAKNPRINSSLVAAIRLAKVSKS-AAAT 219

Query: 77  PQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGV 136
             KV+HF+ NQ+RPDKA+TT+RA+K GKANAASGKI+VTIP DHFGPIPAENDP RNQG+
Sbjct: 220 TSKVFHFISNQDRPDKAFTTERAKKTGKANAASGKIYVTIPPDHFGPIPAENDPVRNQGL 279

Query: 137 LVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGR 196
           LVGE WEDRLECRQWGAHFPHVAGIAGQS  GAQSV LSGGY+DDEDHGEWFLYTGSGGR
Sbjct: 280 LVGESWEDRLECRQWGAHFPHVAGIAGQSTYGAQSVALSGGYKDDEDHGEWFLYTGSGGR 339

Query: 197 DLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIY 256
           DLSGNKRT+K+QSFDQKFEKSN AL++SC  GYPVRVVRSHKEKRS+YAPE+G+RYDG+Y
Sbjct: 340 DLSGNKRTNKEQSFDQKFEKSNAALKLSCKLGYPVRVVRSHKEKRSAYAPEEGVRYDGVY 399

Query: 257 RIEKCWRKVGIQG-FKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 316
           RIEKCWRKVG+QG FKVCRYLFVRCDN+PAPWTSDE+GDRPRP+P IPEL  ATD+ ERK
Sbjct: 400 RIEKCWRKVGVQGSFKVCRYLFVRCDNEPAPWTSDENGDRPRPIPNIPELNMATDLFERK 459

Query: 317 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 376
           E PSWDFDE +  WKW K PP SKK V        K  R+ I+ +H+ ++R RLLKEF C
Sbjct: 460 ETPSWDFDEGEGCWKWMKPPPASKKSVNVLAPEERKNLRKAIKAAHSNTMRARLLKEFKC 519

Query: 377 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 436
             C++V++LP+TTPCAHNFCK CLE  F GKT +RERS+GGR LRS+KNV++CPCCPTDI
Sbjct: 520 QICQQVLTLPVTTPCAHNFCKACLEAKFAGKTLVRERSTGGRTLRSRKNVLNCPCCPTDI 579

Query: 437 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGK 489
           SDFLQN QVNRE+ +VIE LK++ EE  A  E   E E  G   EE+ E+  K
Sbjct: 580 SDFLQNPQVNREVAEVIEKLKTQ-EEDTAELEDEDEGECSGTTPEEDSEQPKK 630

BLAST of Lag0033545 vs. ExPASy Swiss-Prot
Match: Q9FKA7 (E3 ubiquitin-protein ligase ORTHRUS 1 OS=Arabidopsis thaliana OX=3702 GN=ORTH1 PE=1 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 1.1e-183
Identity = 314/475 (66.11%), Postives = 367/475 (77.26%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKW-IGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGS 75
           TPCGHNFCLKCF+KW +GQGK TC  CR  IP  +A  PRIN  LV AIR+A ++K    
Sbjct: 142 TPCGHNFCLKCFEKWAVGQGKLTCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSVE 201

Query: 76  GGPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQ 135
               KV+H + NQ+RP+KA+TT+RA K GKANAASGK FVTIP DHFGPIPAEND  R Q
Sbjct: 202 ATAAKVHHIIRNQDRPEKAFTTERAVKTGKANAASGKFFVTIPRDHFGPIPAENDVTRKQ 261

Query: 136 GVLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSG 195
           GVLVGE WEDR ECRQWGAHFPH+AGIAGQS  GAQSV LSGGY DDEDHGEWFLYTGSG
Sbjct: 262 GVLVGESWEDRQECRQWGAHFPHIAGIAGQSAVGAQSVALSGGYDDDEDHGEWFLYTGSG 321

Query: 196 GRDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDG 255
           GRDLSGNKR +K QS DQ F+  N++L++SC  GYPVRVVRS KEKRS+YAP +G+RYDG
Sbjct: 322 GRDLSGNKRINKKQSSDQAFKNMNESLRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDG 381

Query: 256 IYRIEKCWRKVGIQG-FKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITE 315
           +YRIEKCW  VG+QG FKVCRYLFVRCDN+PAPWTSDEHGDRPRPLP +PEL+ A D+  
Sbjct: 382 VYRIEKCWSNVGVQGSFKVCRYLFVRCDNEPAPWTSDEHGDRPRPLPNVPELETAADLFV 441

Query: 316 RKEGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEF 375
           RKE PSWDFDE + RWKW K+PP S+  ++ E+    KR++         +++ RLLKEF
Sbjct: 442 RKESPSWDFDEAEGRWKWMKSPPVSRMALDPEERKKNKRAKN--------TMKARLLKEF 501

Query: 376 SCLTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPT 435
           SC  CREV+SLP+TTPCAHNFCK CLE  F G T LRERS+GGR LR++KN+M+CPCC T
Sbjct: 502 SCQICREVLSLPVTTPCAHNFCKACLEAKFAGITQLRERSNGGRKLRAKKNIMTCPCCTT 561

Query: 436 DISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGK 489
           D+S+FLQN QVNRE++++IE+ K   EE DAS           EEEEEE E   K
Sbjct: 562 DLSEFLQNPQVNREMMEIIENFKKSEEEADASI---------SEEEEEESEPPTK 599

BLAST of Lag0033545 vs. ExPASy Swiss-Prot
Match: Q680I0 (E3 ubiquitin-protein ligase ORTHRUS 5 OS=Arabidopsis thaliana OX=3702 GN=ORTH5 PE=2 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 7.9e-182
Identity = 312/480 (65.00%), Postives = 372/480 (77.50%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKW-IGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGS 75
           TPCGHNFCLKCF+KW +GQGK TC  CR  IP  +A  PRIN  LV AIR+A ++K  G 
Sbjct: 142 TPCGHNFCLKCFEKWAVGQGKLTCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSGE 201

Query: 76  GGPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQ 135
               KV+H + NQ+RPDKA+TT+RA K GKANAASGK FVTIP DHFGPIPA ND  RNQ
Sbjct: 202 ATAAKVHHIIRNQDRPDKAFTTERAVKTGKANAASGKFFVTIPRDHFGPIPAANDVTRNQ 261

Query: 136 GVLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSG 195
           GVLVGE WEDR ECRQWG HFPHVAGIAGQ+  GAQSV LSGGY DDEDHGEWFLYTGSG
Sbjct: 262 GVLVGESWEDRQECRQWGVHFPHVAGIAGQAAVGAQSVALSGGYDDDEDHGEWFLYTGSG 321

Query: 196 GRDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDG 255
           GRDLSGNKR +K QS DQ F+  N+AL++SC  GYPVRVVRS KEKRS+YAP +G+RYDG
Sbjct: 322 GRDLSGNKRVNKIQSSDQAFKNMNEALRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDG 381

Query: 256 IYRIEKCWRKVGIQGF-KVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITE 315
           +YRIEKCW  VG+QG  K+CRYLFVRCDN+PAPWTSDEHGDRPRPLP +PEL+ ATD+  
Sbjct: 382 VYRIEKCWSNVGVQGLHKMCRYLFVRCDNEPAPWTSDEHGDRPRPLPDVPELENATDLFV 441

Query: 316 RKEGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEF 375
           RKE PSW FDE + RWKW K+PP S+  ++TE+    KR+++      N +++ RLLKEF
Sbjct: 442 RKESPSWGFDEAEGRWKWMKSPPVSRMALDTEERKKNKRAKK-----GNNAMKARLLKEF 501

Query: 376 SCLTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPT 435
           SC  CR+V+SLP+TTPCAHNFCK CLE  F G T LR+RS+G R LR++KN+M+CPCC T
Sbjct: 502 SCQICRKVLSLPVTTPCAHNFCKACLEAKFAGITQLRDRSNGVRKLRAKKNIMTCPCCTT 561

Query: 436 DISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGKDGSSS 494
           D+S+FLQN QVNRE++++IE+ K   EE + +      EE EGEEE E   ++ K   +S
Sbjct: 562 DLSEFLQNPQVNREMMEIIENFKKSEEEAEVAESSNISEE-EGEEESEPPTKKIKMDKNS 615

BLAST of Lag0033545 vs. ExPASy Swiss-Prot
Match: Q9C8E1 (Putative E3 ubiquitin-protein ligase ORTHRUS 4 OS=Arabidopsis thaliana OX=3702 GN=ORTH4 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 1.8e-181
Identity = 314/485 (64.74%), Postives = 376/485 (77.53%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKW-IGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGS 75
           TPCGHNFCLKCF+KW +GQGK TC  CR  IP  +A  PRIN  LV AIR+A ++K  G 
Sbjct: 142 TPCGHNFCLKCFEKWAVGQGKLTCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSGE 201

Query: 76  GGPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQ 135
               KV+H + NQ+RPDKA+TT+RA K GKANAASGK FVTIP DHFGPIPA ND  RNQ
Sbjct: 202 ATAAKVHHIIRNQDRPDKAFTTERAVKTGKANAASGKFFVTIPRDHFGPIPAANDVTRNQ 261

Query: 136 GVLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSG 195
           GVLVGE WEDR ECRQWG HFPHVAGIAGQ+  GAQSV LSGGY DDEDHGEWFLYTGSG
Sbjct: 262 GVLVGESWEDRQECRQWGVHFPHVAGIAGQAAVGAQSVALSGGYDDDEDHGEWFLYTGSG 321

Query: 196 GRDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDG 255
           GRDLSGNKR +K QS DQ F+  N+AL++SC  GYPVRVVRS KEKRS+YAP +G+RYDG
Sbjct: 322 GRDLSGNKRVNKIQSSDQAFKNMNEALRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDG 381

Query: 256 IYRIEKCWRKVGIQGF-KVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITE 315
           +YRIEKCW  VG+QG  K+CRYLFVRCDN+PAPWTSDEHGDRPRPLP +PEL+ ATD+  
Sbjct: 382 VYRIEKCWSNVGVQGLHKMCRYLFVRCDNEPAPWTSDEHGDRPRPLPDVPELENATDLFV 441

Query: 316 RKEGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEF 375
           RKE PSW FDE + RWKW K+PP S+  ++TE+    KR+++      N +++ RLLKEF
Sbjct: 442 RKESPSWGFDEAEGRWKWMKSPPVSRMALDTEERKKNKRAKK-----GNNAMKARLLKEF 501

Query: 376 SCLTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPT 435
           SC  CR+V+SLP+TTPCAHNFCK CLE  F G T LR+RS+G R LR++KN+M+CPCC T
Sbjct: 502 SCQICRKVLSLPVTTPCAHNFCKACLEAKFAGITQLRDRSNGVRKLRAKKNIMTCPCCTT 561

Query: 436 DISDFLQNLQVNRELLDVIESLKSKIEEGD-ASSEKLCEEEIEGEEEEEEEEEEGKDGSS 495
           D+S+FLQN QVNRE++++IE+ K   EE + A S  + EEE   EEE E   ++ K  ++
Sbjct: 562 DLSEFLQNPQVNREMMEIIENFKKSEEEAEVAESSNISEEE---EEESEPPTKKIKMDNN 618

Query: 496 SPVET 498
           S  +T
Sbjct: 622 SVGDT 618

BLAST of Lag0033545 vs. ExPASy Swiss-Prot
Match: Q9FVS2 (E3 ubiquitin-protein ligase ORTHRUS 3 OS=Arabidopsis thaliana OX=3702 GN=ORTH3 PE=3 SV=2)

HSP 1 Score: 624.4 bits (1609), Expect = 1.2e-177
Identity = 308/477 (64.57%), Postives = 369/477 (77.36%), Query Frame = 0

Query: 14  KQTPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIG 73
           + TPCGHN CLKCF KW+GQG  +C  CR VIP  M + PRIN ++V AIR+A++S+   
Sbjct: 169 ESTPCGHNACLKCFLKWMGQGHRSCGTCRSVIPESMVTNPRINLSIVSAIRLARVSEK-A 228

Query: 74  SGGPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERN 133
                KV H+V N++RPDKA+TT+RA+K G ANA+SGKIFVTIP DHFGPIPAENDP RN
Sbjct: 229 DARTSKVVHYVDNEDRPDKAFTTERAKKTGNANASSGKIFVTIPRDHFGPIPAENDPVRN 288

Query: 134 QGVLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGS 193
           QG+LVGE W+ RL CRQWGAHFPHV+GIAGQ++ GAQSVVL+GGY DDEDHGEWFLYTGS
Sbjct: 289 QGLLVGESWKGRLACRQWGAHFPHVSGIAGQASYGAQSVVLAGGYDDDEDHGEWFLYTGS 348

Query: 194 GGRDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKG-LRY 253
           GGR L GNKRT+  Q+FDQ F   N+AL++SC  GYPVRVVRS K+KRS YAP+ G LRY
Sbjct: 349 GGRILKGNKRTNTVQAFDQVFLNFNEALRLSCKLGYPVRVVRSTKDKRSPYAPQGGLLRY 408

Query: 254 DGIYRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDIT 313
           DG+YRIEKCWR VGIQ   +CR+LFVRCDN+PAPWTSDEHGDRPRPLP +PEL  ATD+ 
Sbjct: 409 DGVYRIEKCWRIVGIQ---MCRFLFVRCDNEPAPWTSDEHGDRPRPLPNVPELNMATDLF 468

Query: 314 ERKEGPSWDFDEKDSRWKWNKTPPTSKKPVETE-DSASGKRSRRRIRQSHNMSVRERLLK 373
           ERKE PSWDFDE + RW+W K PP SKK V+   D    K  R  I+ ++  ++R RLLK
Sbjct: 469 ERKESPSWDFDEGEDRWRWMKPPPASKKAVKNVLDPEERKLLREAIKSANPNTMRARLLK 528

Query: 374 EFSCLTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCC 433
           EF C  C++VM+ P+TTPCAHNFCK CLE  F G   +RER SGGR LRSQK+VM CPCC
Sbjct: 529 EFKCQICQKVMTNPVTTPCAHNFCKACLESKFAGTALVRERGSGGRKLRSQKSVMKCPCC 588

Query: 434 PTDISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGK 489
           PTDI++F+QN QVNRE+ +VIE LK + EE +A S  L E +  G   EEE++E+ K
Sbjct: 589 PTDIAEFVQNPQVNREVAEVIEKLKKQEEEENAKS--LDEGQCSGTSHEEEDDEQPK 639

BLAST of Lag0033545 vs. ExPASy TrEMBL
Match: A0A6J1BYU6 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111006998 PE=4 SV=1)

HSP 1 Score: 975.7 bits (2521), Expect = 7.7e-281
Identity = 482/518 (93.05%), Postives = 495/518 (95.56%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSG 75
           TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSI SG
Sbjct: 152 TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIVSG 211

Query: 76  GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 135
           GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG
Sbjct: 212 GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 271

Query: 136 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 195
           VLVGECWEDRLECRQWGAHFPHVAGIAGQS+NGAQSVVLSGGYQDDEDHGEWFLYTGSGG
Sbjct: 272 VLVGECWEDRLECRQWGAHFPHVAGIAGQSSNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 331

Query: 196 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 255
           RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI
Sbjct: 332 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 391

Query: 256 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 315
           YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPR LPAIPELKKATDITERK
Sbjct: 392 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRALPAIPELKKATDITERK 451

Query: 316 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 375
           EGPSWDFDEKDSRWKW+K PPTSKKPVETEDSASGKRSRR++RQS NMSVRE+LLKEFSC
Sbjct: 452 EGPSWDFDEKDSRWKWSKPPPTSKKPVETEDSASGKRSRRKVRQSQNMSVREKLLKEFSC 511

Query: 376 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 435
           L CREVM+LPITTPCAHNFCK+CLEGAFVGKTFLRERSSGGRALRSQKNVM+CPCCPTDI
Sbjct: 512 LICREVMTLPITTPCAHNFCKSCLEGAFVGKTFLRERSSGGRALRSQKNVMTCPCCPTDI 571

Query: 436 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGKDGSSSP- 495
           SDFLQNLQVNRELLDVIESLKSKIEEGD +SEKLCEE  +GEEE +E         SSP 
Sbjct: 572 SDFLQNLQVNRELLDVIESLKSKIEEGD-TSEKLCEEGTDGEEEGKE--------CSSPV 631

Query: 496 -VETRKRAKAVVNGGEENAVGGNGDDSPSSPLHVDDLE 532
            VETRKRAK V +GGEEN VGG+GDDSPSSPLHVDDLE
Sbjct: 632 GVETRKRAKVVADGGEENGVGGDGDDSPSSPLHVDDLE 660

BLAST of Lag0033545 vs. ExPASy TrEMBL
Match: A0A6J1HS20 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465604 PE=4 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 4.5e-273
Identity = 475/522 (91.00%), Postives = 489/522 (93.68%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSG 75
           TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SG
Sbjct: 155 TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISG 214

Query: 76  GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 135
           G QKVYH VHN NRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQG
Sbjct: 215 GSQKVYHVVHNLNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQG 274

Query: 136 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 195
           VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG
Sbjct: 275 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 334

Query: 196 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 255
           RDLSGNKRTSKDQSFDQKFEKSNKALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGI
Sbjct: 335 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGI 394

Query: 256 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 315
           YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK
Sbjct: 395 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 454

Query: 316 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 375
           + PSWDFDEKDSRWKW+K PPTSKK VETEDS SGK+SRRR+RQSHNM+VRERLLKEFSC
Sbjct: 455 KDPSWDFDEKDSRWKWSKPPPTSKKSVETEDSTSGKKSRRRMRQSHNMNVRERLLKEFSC 514

Query: 376 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 435
           L CREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS+KNVM+CPCCPTDI
Sbjct: 515 LICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRSRKNVMACPCCPTDI 574

Query: 436 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGE-EEEEEEEEEGKDG---- 495
           SDFLQNLQVNRELLDVIESLKSKIEE DA SEK CE+EIEGE E E E+EEEGKDG    
Sbjct: 575 SDFLQNLQVNRELLDVIESLKSKIEEADA-SEKPCEDEIEGEGEGEGEQEEEGKDGSNGN 634

Query: 496 -SSSPVETRKRAKAVVNGGEENAVGGNGDDSPSSPLHVDDLE 532
            SS PVETRKRAK VVNGGEEN       DSPSSP+HVDDLE
Sbjct: 635 ESSRPVETRKRAKVVVNGGEEN-------DSPSSPVHVDDLE 668

BLAST of Lag0033545 vs. ExPASy TrEMBL
Match: A0A6J1F0L5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441068 PE=4 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 2.9e-272
Identity = 473/526 (89.92%), Postives = 490/526 (93.16%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSG 75
           TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMAS PRINSTLVVAIRMAKMSKSI SG
Sbjct: 155 TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASHPRINSTLVVAIRMAKMSKSIISG 214

Query: 76  GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 135
           G QKVYH VHN NRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPI AENDPERNQG
Sbjct: 215 GSQKVYHVVHNLNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPILAENDPERNQG 274

Query: 136 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 195
           VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG
Sbjct: 275 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 334

Query: 196 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 255
           RDLSGNKRTSKDQSFDQKFEKSNKALQVSCL GYPVRVVRSHKEKRSSYAPEKGLRYDGI
Sbjct: 335 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLNGYPVRVVRSHKEKRSSYAPEKGLRYDGI 394

Query: 256 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 315
           YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK
Sbjct: 395 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 454

Query: 316 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 375
           + PSWDFDEKD+RWKW+K PPTSKK VETEDS SGK+SRRR+RQSHNM+VRERLLKEFSC
Sbjct: 455 KDPSWDFDEKDNRWKWSKPPPTSKKSVETEDSTSGKKSRRRMRQSHNMNVRERLLKEFSC 514

Query: 376 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 435
           L CREVM+LPITTPCAHNFCK+CLEGAF GK+FLRER+SGGRALRS+KNVM+CPCCPTDI
Sbjct: 515 LICREVMNLPITTPCAHNFCKSCLEGAFAGKSFLRERNSGGRALRSRKNVMACPCCPTDI 574

Query: 436 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEG-----EEEEEEEEEEGKDG 495
           SDFLQNLQVNRELLDVIESLKSKIEE DA SEK CE+EIEG     EE+E+E+EEEGKDG
Sbjct: 575 SDFLQNLQVNRELLDVIESLKSKIEEADA-SEKPCEDEIEGEGEGEEEQEQEQEEEGKDG 634

Query: 496 -----SSSPVETRKRAKAVVNGGEENAVGGNGDDSPSSPLHVDDLE 532
                SS PVETRKRAK VVNGGEEN        SPSSP+HVDDLE
Sbjct: 635 SNGNESSRPVETRKRAKVVVNGGEENY-------SPSSPVHVDDLE 672

BLAST of Lag0033545 vs. ExPASy TrEMBL
Match: A0A1S3B4H1 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486058 PE=4 SV=1)

HSP 1 Score: 922.9 bits (2384), Expect = 5.9e-265
Identity = 448/498 (89.96%), Postives = 470/498 (94.38%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSG 75
           TPCGHNFCLKCFQKWIG GKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAK+SKS  SG
Sbjct: 155 TPCGHNFCLKCFQKWIGLGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKLSKSSVSG 214

Query: 76  GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 135
           GPQK YHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG
Sbjct: 215 GPQKAYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 274

Query: 136 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 195
           VLVGECWEDRLECRQWGAHFPH+AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG
Sbjct: 275 VLVGECWEDRLECRQWGAHFPHIAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 334

Query: 196 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 255
           RDLSGNKRTSKDQSFDQKFEK NKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI
Sbjct: 335 RDLSGNKRTSKDQSFDQKFEKYNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 394

Query: 256 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 315
           YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSD+HGDRPRPLP+IPELKKAT+ITERK
Sbjct: 395 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDDHGDRPRPLPSIPELKKATNITERK 454

Query: 316 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 375
           EGPSWDFDEKDS+WKW+K PP S +PVETED A+GKRSRR+ RQ+HNM+VRERLLKEFSC
Sbjct: 455 EGPSWDFDEKDSQWKWSKPPPISTRPVETEDPATGKRSRRKFRQAHNMNVRERLLKEFSC 514

Query: 376 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 435
           L CREVMSLPITTPCAHNFCK+CLEGAF GKTFLRERSSGGR+LRS+KNVM+CPCCPTDI
Sbjct: 515 LICREVMSLPITTPCAHNFCKSCLEGAFAGKTFLRERSSGGRSLRSRKNVMACPCCPTDI 574

Query: 436 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGKDGSSSPV 495
           SDFLQNLQVNRELLDVIESLK K+EE   +SEKLCEEEI+GEEEEEEEE E +      V
Sbjct: 575 SDFLQNLQVNRELLDVIESLKGKLEEEGDASEKLCEEEIDGEEEEEEEEGEDE------V 634

Query: 496 ETRKRAKAVVNGGEENAV 514
           E RK+AK  +NGGEEN V
Sbjct: 635 ENRKQAKMAINGGEENPV 646

BLAST of Lag0033545 vs. ExPASy TrEMBL
Match: A0A0A0L3V5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G507950 PE=4 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 5.0e-264
Identity = 447/503 (88.87%), Postives = 472/503 (93.84%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSG 75
           TPCGHNFCLKCFQKWIG GK TCAKCRCVIP+KMASQPRINSTLVVAIRMAK+SKS  SG
Sbjct: 155 TPCGHNFCLKCFQKWIGLGKKTCAKCRCVIPTKMASQPRINSTLVVAIRMAKLSKSSVSG 214

Query: 76  GPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 135
           GPQK YHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG
Sbjct: 215 GPQKAYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQG 274

Query: 136 VLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 195
           VLVGECWEDRLECRQWGAHFPH+AGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG
Sbjct: 275 VLVGECWEDRLECRQWGAHFPHIAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGG 334

Query: 196 RDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 255
           RDLSGNKRTSKDQSFDQKFEK NKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI
Sbjct: 335 RDLSGNKRTSKDQSFDQKFEKYNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGI 394

Query: 256 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 315
           YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSD+HGDRPRPLP+IPELKKAT+ITERK
Sbjct: 395 YRIEKCWRKVGIQGFKVCRYLFVRCDNDPAPWTSDDHGDRPRPLPSIPELKKATNITERK 454

Query: 316 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 375
           EGPSWDFDEKDS+WKW+K PP S +PVETED A+GKRSRR+IRQSHNM+VRERLLKEFSC
Sbjct: 455 EGPSWDFDEKDSQWKWSKPPPMSTRPVETEDPATGKRSRRKIRQSHNMNVRERLLKEFSC 514

Query: 376 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 435
           L CREVMS+PITTPCAHNFCK+CLEGAF GKTFLRERSSGGR+LRS+KNVM+CPCCPTDI
Sbjct: 515 LICREVMSMPITTPCAHNFCKSCLEGAFAGKTFLRERSSGGRSLRSRKNVMACPCCPTDI 574

Query: 436 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEE-----EEGKDG 495
           SDFLQNLQVNRELLDVIESLK K+EE   +SEKLCEEE+E EEEEEEEE     EE ++ 
Sbjct: 575 SDFLQNLQVNRELLDVIESLKGKLEEEGDASEKLCEEEMEEEEEEEEEEKEEEKEEEEEE 634

Query: 496 SSSPVETRKRAKAVVNGGEENAV 514
               VE+RK+AK  VNGGEEN +
Sbjct: 635 GKGEVESRKQAKVEVNGGEENPI 657

BLAST of Lag0033545 vs. TAIR 10
Match: AT1G57820.1 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 703.0 bits (1813), Expect = 1.9e-202
Identity = 340/473 (71.88%), Postives = 392/473 (82.88%), Query Frame = 0

Query: 17  PCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGG 76
           PCGHN CLKCF+KW+GQGK TC KCR +IP KMA  PRINS+LV AIR+AK+SKS  +  
Sbjct: 160 PCGHNACLKCFEKWMGQGKRTCGKCRSIIPEKMAKNPRINSSLVAAIRLAKVSKS-AAAT 219

Query: 77  PQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGV 136
             KV+HF+ NQ+RPDKA+TT+RA+K GKANAASGKI+VTIP DHFGPIPAENDP RNQG+
Sbjct: 220 TSKVFHFISNQDRPDKAFTTERAKKTGKANAASGKIYVTIPPDHFGPIPAENDPVRNQGL 279

Query: 137 LVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGR 196
           LVGE WEDRLECRQWGAHFPHVAGIAGQS  GAQSV LSGGY+DDEDHGEWFLYTGSGGR
Sbjct: 280 LVGESWEDRLECRQWGAHFPHVAGIAGQSTYGAQSVALSGGYKDDEDHGEWFLYTGSGGR 339

Query: 197 DLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIY 256
           DLSGNKRT+K+QSFDQKFEKSN AL++SC  GYPVRVVRSHKEKRS+YAPE+G+RYDG+Y
Sbjct: 340 DLSGNKRTNKEQSFDQKFEKSNAALKLSCKLGYPVRVVRSHKEKRSAYAPEEGVRYDGVY 399

Query: 257 RIEKCWRKVGIQG-FKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 316
           RIEKCWRKVG+QG FKVCRYLFVRCDN+PAPWTSDE+GDRPRP+P IPEL  ATD+ ERK
Sbjct: 400 RIEKCWRKVGVQGSFKVCRYLFVRCDNEPAPWTSDENGDRPRPIPNIPELNMATDLFERK 459

Query: 317 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 376
           E PSWDFDE +  WKW K PP SKK V        K  R+ I+ +H+ ++R RLLKEF C
Sbjct: 460 ETPSWDFDEGEGCWKWMKPPPASKKSVNVLAPEERKNLRKAIKAAHSNTMRARLLKEFKC 519

Query: 377 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 436
             C++V++LP+TTPCAHNFCK CLE  F GKT +RERS+GGR LRS+KNV++CPCCPTDI
Sbjct: 520 QICQQVLTLPVTTPCAHNFCKACLEAKFAGKTLVRERSTGGRTLRSRKNVLNCPCCPTDI 579

Query: 437 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGK 489
           SDFLQN QVNRE+ +VIE LK++ EE  A  E   E E  G   EE+ E+  K
Sbjct: 580 SDFLQNPQVNREVAEVIEKLKTQ-EEDTAELEDEDEGECSGTTPEEDSEQPKK 630

BLAST of Lag0033545 vs. TAIR 10
Match: AT1G57820.2 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 692.2 bits (1785), Expect = 3.3e-199
Identity = 337/473 (71.25%), Postives = 389/473 (82.24%), Query Frame = 0

Query: 17  PCGHNFCLKCFQKWIGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGSGG 76
           PCGHN CLKCF+KW+GQGK TC KCR +IP KMA  PRINS+LV AIR+AK+SKS  +  
Sbjct: 160 PCGHNACLKCFEKWMGQGKRTCGKCRSIIPEKMAKNPRINSSLVAAIRLAKVSKS-AAAT 219

Query: 77  PQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQGV 136
             KV+HF+ NQ+RPDKA+TT+RA+K GKANAASGKI+VTIP DHFGPIPAENDP RNQG+
Sbjct: 220 TSKVFHFISNQDRPDKAFTTERAKKTGKANAASGKIYVTIPPDHFGPIPAENDPVRNQGL 279

Query: 137 LVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSGGR 196
           LVGE WEDRLECRQWGAHFPHVAGIAGQS  GAQSV LSGGY+DDEDHGEWFLYT   GR
Sbjct: 280 LVGESWEDRLECRQWGAHFPHVAGIAGQSTYGAQSVALSGGYKDDEDHGEWFLYT---GR 339

Query: 197 DLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDGIY 256
           DLSGNKRT+K+QSFDQKFEKSN AL++SC  GYPVRVVRSHKEKRS+YAPE+G+RYDG+Y
Sbjct: 340 DLSGNKRTNKEQSFDQKFEKSNAALKLSCKLGYPVRVVRSHKEKRSAYAPEEGVRYDGVY 399

Query: 257 RIEKCWRKVGIQG-FKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITERK 316
           RIEKCWRKVG+QG FKVCRYLFVRCDN+PAPWTSDE+GDRPRP+P IPEL  ATD+ ERK
Sbjct: 400 RIEKCWRKVGVQGSFKVCRYLFVRCDNEPAPWTSDENGDRPRPIPNIPELNMATDLFERK 459

Query: 317 EGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEFSC 376
           E PSWDFDE +  WKW K PP SKK V        K  R+ I+ +H+ ++R RLLKEF C
Sbjct: 460 ETPSWDFDEGEGCWKWMKPPPASKKSVNVLAPEERKNLRKAIKAAHSNTMRARLLKEFKC 519

Query: 377 LTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPTDI 436
             C++V++LP+TTPCAHNFCK CLE  F GKT +RERS+GGR LRS+KNV++CPCCPTDI
Sbjct: 520 QICQQVLTLPVTTPCAHNFCKACLEAKFAGKTLVRERSTGGRTLRSRKNVLNCPCCPTDI 579

Query: 437 SDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGK 489
           SDFLQN QVNRE+ +VIE LK++ EE  A  E   E E  G   EE+ E+  K
Sbjct: 580 SDFLQNPQVNREVAEVIEKLKTQ-EEDTAELEDEDEGECSGTTPEEDSEQPKK 627

BLAST of Lag0033545 vs. TAIR 10
Match: AT5G39550.1 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 644.4 bits (1661), Expect = 7.8e-185
Identity = 314/475 (66.11%), Postives = 367/475 (77.26%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKW-IGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGS 75
           TPCGHNFCLKCF+KW +GQGK TC  CR  IP  +A  PRIN  LV AIR+A ++K    
Sbjct: 142 TPCGHNFCLKCFEKWAVGQGKLTCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSVE 201

Query: 76  GGPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQ 135
               KV+H + NQ+RP+KA+TT+RA K GKANAASGK FVTIP DHFGPIPAEND  R Q
Sbjct: 202 ATAAKVHHIIRNQDRPEKAFTTERAVKTGKANAASGKFFVTIPRDHFGPIPAENDVTRKQ 261

Query: 136 GVLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSG 195
           GVLVGE WEDR ECRQWGAHFPH+AGIAGQS  GAQSV LSGGY DDEDHGEWFLYTGSG
Sbjct: 262 GVLVGESWEDRQECRQWGAHFPHIAGIAGQSAVGAQSVALSGGYDDDEDHGEWFLYTGSG 321

Query: 196 GRDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDG 255
           GRDLSGNKR +K QS DQ F+  N++L++SC  GYPVRVVRS KEKRS+YAP +G+RYDG
Sbjct: 322 GRDLSGNKRINKKQSSDQAFKNMNESLRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDG 381

Query: 256 IYRIEKCWRKVGIQG-FKVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITE 315
           +YRIEKCW  VG+QG FKVCRYLFVRCDN+PAPWTSDEHGDRPRPLP +PEL+ A D+  
Sbjct: 382 VYRIEKCWSNVGVQGSFKVCRYLFVRCDNEPAPWTSDEHGDRPRPLPNVPELETAADLFV 441

Query: 316 RKEGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEF 375
           RKE PSWDFDE + RWKW K+PP S+  ++ E+    KR++         +++ RLLKEF
Sbjct: 442 RKESPSWDFDEAEGRWKWMKSPPVSRMALDPEERKKNKRAKN--------TMKARLLKEF 501

Query: 376 SCLTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPT 435
           SC  CREV+SLP+TTPCAHNFCK CLE  F G T LRERS+GGR LR++KN+M+CPCC T
Sbjct: 502 SCQICREVLSLPVTTPCAHNFCKACLEAKFAGITQLRERSNGGRKLRAKKNIMTCPCCTT 561

Query: 436 DISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGK 489
           D+S+FLQN QVNRE++++IE+ K   EE DAS           EEEEEE E   K
Sbjct: 562 DLSEFLQNPQVNREMMEIIENFKKSEEEADASI---------SEEEEEESEPPTK 599

BLAST of Lag0033545 vs. TAIR 10
Match: AT1G66050.1 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 638.3 bits (1645), Expect = 5.6e-183
Identity = 312/480 (65.00%), Postives = 372/480 (77.50%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKW-IGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGS 75
           TPCGHNFCLKCF+KW +GQGK TC  CR  IP  +A  PRIN  LV AIR+A ++K  G 
Sbjct: 142 TPCGHNFCLKCFEKWAVGQGKLTCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSGE 201

Query: 76  GGPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQ 135
               KV+H + NQ+RPDKA+TT+RA K GKANAASGK FVTIP DHFGPIPA ND  RNQ
Sbjct: 202 ATAAKVHHIIRNQDRPDKAFTTERAVKTGKANAASGKFFVTIPRDHFGPIPAANDVTRNQ 261

Query: 136 GVLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSG 195
           GVLVGE WEDR ECRQWG HFPHVAGIAGQ+  GAQSV LSGGY DDEDHGEWFLYTGSG
Sbjct: 262 GVLVGESWEDRQECRQWGVHFPHVAGIAGQAAVGAQSVALSGGYDDDEDHGEWFLYTGSG 321

Query: 196 GRDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDG 255
           GRDLSGNKR +K QS DQ F+  N+AL++SC  GYPVRVVRS KEKRS+YAP +G+RYDG
Sbjct: 322 GRDLSGNKRVNKIQSSDQAFKNMNEALRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDG 381

Query: 256 IYRIEKCWRKVGIQGF-KVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITE 315
           +YRIEKCW  VG+QG  K+CRYLFVRCDN+PAPWTSDEHGDRPRPLP +PEL+ ATD+  
Sbjct: 382 VYRIEKCWSNVGVQGLHKMCRYLFVRCDNEPAPWTSDEHGDRPRPLPDVPELENATDLFV 441

Query: 316 RKEGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEF 375
           RKE PSW FDE + RWKW K+PP S+  ++TE+    KR+++      N +++ RLLKEF
Sbjct: 442 RKESPSWGFDEAEGRWKWMKSPPVSRMALDTEERKKNKRAKK-----GNNAMKARLLKEF 501

Query: 376 SCLTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPT 435
           SC  CR+V+SLP+TTPCAHNFCK CLE  F G T LR+RS+G R LR++KN+M+CPCC T
Sbjct: 502 SCQICRKVLSLPVTTPCAHNFCKACLEAKFAGITQLRDRSNGVRKLRAKKNIMTCPCCTT 561

Query: 436 DISDFLQNLQVNRELLDVIESLKSKIEEGDASSEKLCEEEIEGEEEEEEEEEEGKDGSSS 494
           D+S+FLQN QVNRE++++IE+ K   EE + +      EE EGEEE E   ++ K   +S
Sbjct: 562 DLSEFLQNPQVNREMMEIIENFKKSEEEAEVAESSNISEE-EGEEESEPPTKKIKMDKNS 615

BLAST of Lag0033545 vs. TAIR 10
Match: AT1G66040.1 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 637.1 bits (1642), Expect = 1.3e-182
Identity = 314/485 (64.74%), Postives = 376/485 (77.53%), Query Frame = 0

Query: 16  TPCGHNFCLKCFQKW-IGQGKNTCAKCRCVIPSKMASQPRINSTLVVAIRMAKMSKSIGS 75
           TPCGHNFCLKCF+KW +GQGK TC  CR  IP  +A  PRIN  LV AIR+A ++K  G 
Sbjct: 142 TPCGHNFCLKCFEKWAVGQGKLTCMICRSKIPRHVAKNPRINLALVSAIRLANVTKCSGE 201

Query: 76  GGPQKVYHFVHNQNRPDKAYTTDRAQKKGKANAASGKIFVTIPSDHFGPIPAENDPERNQ 135
               KV+H + NQ+RPDKA+TT+RA K GKANAASGK FVTIP DHFGPIPA ND  RNQ
Sbjct: 202 ATAAKVHHIIRNQDRPDKAFTTERAVKTGKANAASGKFFVTIPRDHFGPIPAANDVTRNQ 261

Query: 136 GVLVGECWEDRLECRQWGAHFPHVAGIAGQSNNGAQSVVLSGGYQDDEDHGEWFLYTGSG 195
           GVLVGE WEDR ECRQWG HFPHVAGIAGQ+  GAQSV LSGGY DDEDHGEWFLYTGSG
Sbjct: 262 GVLVGESWEDRQECRQWGVHFPHVAGIAGQAAVGAQSVALSGGYDDDEDHGEWFLYTGSG 321

Query: 196 GRDLSGNKRTSKDQSFDQKFEKSNKALQVSCLKGYPVRVVRSHKEKRSSYAPEKGLRYDG 255
           GRDLSGNKR +K QS DQ F+  N+AL++SC  GYPVRVVRS KEKRS+YAP +G+RYDG
Sbjct: 322 GRDLSGNKRVNKIQSSDQAFKNMNEALRLSCKMGYPVRVVRSWKEKRSAYAPAEGVRYDG 381

Query: 256 IYRIEKCWRKVGIQGF-KVCRYLFVRCDNDPAPWTSDEHGDRPRPLPAIPELKKATDITE 315
           +YRIEKCW  VG+QG  K+CRYLFVRCDN+PAPWTSDEHGDRPRPLP +PEL+ ATD+  
Sbjct: 382 VYRIEKCWSNVGVQGLHKMCRYLFVRCDNEPAPWTSDEHGDRPRPLPDVPELENATDLFV 441

Query: 316 RKEGPSWDFDEKDSRWKWNKTPPTSKKPVETEDSASGKRSRRRIRQSHNMSVRERLLKEF 375
           RKE PSW FDE + RWKW K+PP S+  ++TE+    KR+++      N +++ RLLKEF
Sbjct: 442 RKESPSWGFDEAEGRWKWMKSPPVSRMALDTEERKKNKRAKK-----GNNAMKARLLKEF 501

Query: 376 SCLTCREVMSLPITTPCAHNFCKTCLEGAFVGKTFLRERSSGGRALRSQKNVMSCPCCPT 435
           SC  CR+V+SLP+TTPCAHNFCK CLE  F G T LR+RS+G R LR++KN+M+CPCC T
Sbjct: 502 SCQICRKVLSLPVTTPCAHNFCKACLEAKFAGITQLRDRSNGVRKLRAKKNIMTCPCCTT 561

Query: 436 DISDFLQNLQVNRELLDVIESLKSKIEEGD-ASSEKLCEEEIEGEEEEEEEEEEGKDGSS 495
           D+S+FLQN QVNRE++++IE+ K   EE + A S  + EEE   EEE E   ++ K  ++
Sbjct: 562 DLSEFLQNPQVNREMMEIIENFKKSEEEAEVAESSNISEEE---EEESEPPTKKIKMDNN 618

Query: 496 SPVET 498
           S  +T
Sbjct: 622 SVGDT 618

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134816.11.6e-28093.05E3 ubiquitin-protein ligase ORTHRUS 2-like isoform X1 [Momordica charantia][more]
XP_023531327.17.2e-27390.80E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita pepo subsp. pepo][more]
XP_022965844.19.4e-27391.00E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita maxima][more]
KAG6587385.12.7e-27290.61E3 ubiquitin-protein ligase ORTHRUS 2, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022933744.16.1e-27289.92E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8VYZ02.6e-20171.88E3 ubiquitin-protein ligase ORTHRUS 2 OS=Arabidopsis thaliana OX=3702 GN=ORTH2 P... [more]
Q9FKA71.1e-18366.11E3 ubiquitin-protein ligase ORTHRUS 1 OS=Arabidopsis thaliana OX=3702 GN=ORTH1 P... [more]
Q680I07.9e-18265.00E3 ubiquitin-protein ligase ORTHRUS 5 OS=Arabidopsis thaliana OX=3702 GN=ORTH5 P... [more]
Q9C8E11.8e-18164.74Putative E3 ubiquitin-protein ligase ORTHRUS 4 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9FVS21.2e-17764.57E3 ubiquitin-protein ligase ORTHRUS 3 OS=Arabidopsis thaliana OX=3702 GN=ORTH3 P... [more]
Match NameE-valueIdentityDescription
A0A6J1BYU67.7e-28193.05RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11100699... [more]
A0A6J1HS204.5e-27391.00RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465604 P... [more]
A0A6J1F0L52.9e-27289.92RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441068... [more]
A0A1S3B4H15.9e-26589.96RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486058 PE=4 ... [more]
A0A0A0L3V55.0e-26488.87RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G507950 PE... [more]
Match NameE-valueIdentityDescription
AT1G57820.11.9e-20271.88Zinc finger (C3HC4-type RING finger) family protein [more]
AT1G57820.23.3e-19971.25Zinc finger (C3HC4-type RING finger) family protein [more]
AT5G39550.17.8e-18566.11Zinc finger (C3HC4-type RING finger) family protein [more]
AT1G66050.15.6e-18365.00Zinc finger (C3HC4-type RING finger) family protein [more]
AT1G66040.11.3e-18264.74Zinc finger (C3HC4-type RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003105SRA-YDGSMARTSM00466G9a_1coord: 126..283
e-value: 1.8E-75
score: 266.7
IPR003105SRA-YDGPFAMPF02182SAD_SRAcoord: 134..282
e-value: 6.7E-54
score: 181.8
IPR003105SRA-YDGPROSITEPS51015YDGcoord: 131..279
score: 52.585644
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 375..431
e-value: 9.6E-5
score: 31.8
coord: 3..41
e-value: 0.27
score: 16.1
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 375..431
score: 9.937009
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 2..42
score: 9.435407
IPR018957Zinc finger, C3HC4 RING-typePFAMPF00097zf-C3HC4coord: 14..41
e-value: 1.4E-4
score: 21.7
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 330..463
e-value: 1.6E-15
score: 58.7
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 2..80
e-value: 8.9E-10
score: 40.6
IPR027370RING-type zinc-finger, LisH dimerisation motifPFAMPF13445zf-RING_UBOXcoord: 375..429
e-value: 1.5E-6
score: 28.1
IPR036987SRA-YDG superfamilyGENE3D2.30.280.10coord: 102..298
e-value: 1.1E-78
score: 265.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 462..531
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 466..489
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 287..333
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 287..360
NoneNo IPR availablePANTHERPTHR14140:SF42FINGER PROTEIN, PUTATIVE-RELATEDcoord: 15..499
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 368..456
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 13..53
IPR045134UHRF1/2-likePANTHERPTHR14140E3 UBIQUITIN-PROTEIN LIGASE UHRF-RELATEDcoord: 15..499
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 390..399
IPR015947PUA-like superfamilySUPERFAMILY88697PUA domain-likecoord: 105..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033545.1Lag0033545.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006325 chromatin organization
biological_process GO:0010216 maintenance of DNA methylation
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0061630 ubiquitin protein ligase activity