Lag0033544 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0033544
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr3: 195680 .. 196405 (+)
RNA-Seq Expression	Lag0033544
Synteny	Lag0033544

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCAGGTTTCTCAGCTGCCTTGCGACGGCGATGGCATTTGCATGCTCTGCCAGGCCAAGCCTTCCGACTTGGAGACGATCACTTGCAAAACTTGCGTCACCCCGTGGCACGTCGGTTGTCTCTCAAATCCCCCTGAAACTCTGGCATCTACCCTTCAATGGGACTGTCCCGACTGTTCCCCCCCTCCCGAAGATGCTCCCCTTCCTCCACCCGGCAACTGTCCACTCCCCACTGCGCCTTCCAGTGACCTGGTTGCTGCGATTCGAGCCATTGAGGCCGATGCCTCCCTTACTGACCGCGAGAAGGCAAAGAAGCGCCAGGAGCTTCTTAGCGGAAAGTCTCAGTCCGAGAAGGATGGTCATGATGCCAACAAGGAAAAGAGAAAAAGAGGGGATGATGTTTTGGATTTGTTCGACGAGAGATTGAACTGCTCCTTCTGCATGCAGTTACCAGAAAGGCCAGTCACTGTAAGTTCCCTATTGCATCGTTCTTTATCTTCTTTGACATGTCAGACCACCTGCTCCTTTCTTCTTGCCCTTGCATTGCTTGCAAGACTCTTTAACCGTGTCTTTAGCTGTCTGTGTCATCTCACAAATGACATTTCATCTTCCATCTCATCCATTTTTATTTTCGATTCACTTGTTTTAACGAAGAGTTATTTTGACTGTAATCTGTAAAATTCATTTTTACAGTATCACTTCAATTCGTCTTCTGTTCAATAG

mRNA sequence

ATGGCGCAGGTTTCTCAGCTGCCTTGCGACGGCGATGGCATTTGCATGCTCTGCCAGGCCAAGCCTTCCGACTTGGAGACGATCACTTGCAAAACTTGCGTCACCCCGTGGCACGTCGGTTGTCTCTCAAATCCCCCTGAAACTCTGGCATCTACCCTTCAATGGGACTGTCCCGACTGTTCCCCCCCTCCCGAAGATGCTCCCCTTCCTCCACCCGGCAACTGTCCACTCCCCACTGCGCCTTCCAGTGACCTGGTTGCTGCGATTCGAGCCATTGAGGCCGATGCCTCCCTTACTGACCGCGAGAAGGCAAAGAAGCGCCAGGAGCTTCTTAGCGGAAAGTCTCAGTCCGAGAAGGATGGTCATGATGCCAACAAGGAAAAGAGAAAAAGAGGGGATGATGTTTTGGATTTGTTCGACGAGAGATTGAACTGCTCCTTCTGCATGCAGTTACCAGAAAGGCCAGTCACTTATCACTTCAATTCGTCTTCTGTTCAATAG

Coding sequence (CDS)

ATGGCGCAGGTTTCTCAGCTGCCTTGCGACGGCGATGGCATTTGCATGCTCTGCCAGGCCAAGCCTTCCGACTTGGAGACGATCACTTGCAAAACTTGCGTCACCCCGTGGCACGTCGGTTGTCTCTCAAATCCCCCTGAAACTCTGGCATCTACCCTTCAATGGGACTGTCCCGACTGTTCCCCCCCTCCCGAAGATGCTCCCCTTCCTCCACCCGGCAACTGTCCACTCCCCACTGCGCCTTCCAGTGACCTGGTTGCTGCGATTCGAGCCATTGAGGCCGATGCCTCCCTTACTGACCGCGAGAAGGCAAAGAAGCGCCAGGAGCTTCTTAGCGGAAAGTCTCAGTCCGAGAAGGATGGTCATGATGCCAACAAGGAAAAGAGAAAAAGAGGGGATGATGTTTTGGATTTGTTCGACGAGAGATTGAACTGCTCCTTCTGCATGCAGTTACCAGAAAGGCCAGTCACTTATCACTTCAATTCGTCTTCTGTTCAATAG

Protein sequence

MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDCSPPPEDAPLPPPGNCPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKDGHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVTYHFNSSSVQ

Homology

[Close]

BLAST of Lag0033544 vs. NCBI nr
Match: XP_022933744.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita moschata])

HSP 1 Score: 285.8 bits (730), Expect = 2.4e-73
Identity = 141/157 (89.81%), Postives = 145/157 (92.36%), Query Frame = 0


Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MAQVSQLPCDGDGICM CQAKPSDLETITCKTCV PWHVGCLSNPP+ LASTLQWDCPDC
Sbjct: 1   MAQVSQLPCDGDGICMRCQAKPSDLETITCKTCVAPWHVGCLSNPPQILASTLQWDCPDC 60

Query: 61  SPPPEDAPLPPPGNCPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           SPPPEDA LPPP +CPLP  PS+DLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD
Sbjct: 61  SPPPEDASLPPPASCPLPVTPSNDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120

Query: 121 GHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVT 158
            HDAN+   KRGDDVLDLFDERL CSFCMQLPERPVT
Sbjct: 121 DHDANE---KRGDDVLDLFDERLKCSFCMQLPERPVT 154

[Close]

BLAST of Lag0033544 vs. NCBI nr
Match: XP_023531327.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 285.8 bits (730), Expect = 2.4e-73
Identity = 141/157 (89.81%), Postives = 145/157 (92.36%), Query Frame = 0


Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MAQVSQLPCDGDGICM CQAKPSDLETITCKTCV PWHVGCLSNPP+ LASTLQWDCPDC
Sbjct: 1   MAQVSQLPCDGDGICMRCQAKPSDLETITCKTCVAPWHVGCLSNPPQILASTLQWDCPDC 60

Query: 61  SPPPEDAPLPPPGNCPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           SPPPEDA LPPP +CPLP  PS+DLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD
Sbjct: 61  SPPPEDASLPPPASCPLPVTPSNDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120

Query: 121 GHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVT 158
            HDAN+   KRGDDVLDLFDERL CSFCMQLPERPVT
Sbjct: 121 DHDANE---KRGDDVLDLFDERLKCSFCMQLPERPVT 154

[Close]

BLAST of Lag0033544 vs. NCBI nr
Match: KAG6587385.1 (E3 ubiquitin-protein ligase ORTHRUS 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021368.1 E3 ubiquitin-protein ligase ORTHRUS 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 285.8 bits (730), Expect = 2.4e-73
Identity = 141/157 (89.81%), Postives = 145/157 (92.36%), Query Frame = 0


Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MAQVSQLPCDGDGICM CQAKPSDLETITCKTCV PWHVGCLSNPP+ LASTLQWDCPDC
Sbjct: 1   MAQVSQLPCDGDGICMRCQAKPSDLETITCKTCVAPWHVGCLSNPPQILASTLQWDCPDC 60

Query: 61  SPPPEDAPLPPPGNCPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           SPPPEDA LPPP +CPLP  PS+DLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD
Sbjct: 61  SPPPEDASLPPPASCPLPVTPSNDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120

Query: 121 GHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVT 158
            HDAN+   KRGDDVLDLFDERL CSFCMQLPERPVT
Sbjct: 121 DHDANE---KRGDDVLDLFDERLKCSFCMQLPERPVT 154

[Close]

BLAST of Lag0033544 vs. NCBI nr
Match: XP_022994127.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 284.6 bits (727), Expect = 5.3e-73
Identity = 139/157 (88.54%), Postives = 145/157 (92.36%), Query Frame = 0


Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MA  +QLPCDGDGICMLC+AKPSDLETITCKTCVTPWHV CLS+PPETLASTLQWDCPDC
Sbjct: 16  MASKTQLPCDGDGICMLCKAKPSDLETITCKTCVTPWHVDCLSSPPETLASTLQWDCPDC 75

Query: 61  SPPPEDAPLPPPGNCPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S PPED PLPP GN PLPT PSSDLVAAIRAIEAD SLTDREKAKKRQELLSGK QS+KD
Sbjct: 76  SSPPEDVPLPPIGNNPLPTTPSSDLVAAIRAIEADVSLTDREKAKKRQELLSGKMQSDKD 135

Query: 121 GHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVT 158
            HDANKEK+K+GDDVLDL DERLNCSFCMQLPERPVT
Sbjct: 136 DHDANKEKKKKGDDVLDLLDERLNCSFCMQLPERPVT 172

[Close]

BLAST of Lag0033544 vs. NCBI nr
Match: XP_022994113.1 (E3 ubiquitin-protein ligase ORTHRUS 2-like isoform X1 [Cucurbita maxima] >XP_022994120.1 E3 ubiquitin-protein ligase ORTHRUS 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 284.6 bits (727), Expect = 5.3e-73
Identity = 139/157 (88.54%), Postives = 145/157 (92.36%), Query Frame = 0


Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MA  +QLPCDGDGICMLC+AKPSDLETITCKTCVTPWHV CLS+PPETLASTLQWDCPDC
Sbjct: 16  MASKTQLPCDGDGICMLCKAKPSDLETITCKTCVTPWHVDCLSSPPETLASTLQWDCPDC 75

Query: 61  SPPPEDAPLPPPGNCPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S PPED PLPP GN PLPT PSSDLVAAIRAIEAD SLTDREKAKKRQELLSGK QS+KD
Sbjct: 76  SSPPEDVPLPPIGNNPLPTTPSSDLVAAIRAIEADVSLTDREKAKKRQELLSGKMQSDKD 135

Query: 121 GHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVT 158
            HDANKEK+K+GDDVLDL DERLNCSFCMQLPERPVT
Sbjct: 136 DHDANKEKKKKGDDVLDLLDERLNCSFCMQLPERPVT 172

[Close]

BLAST of Lag0033544 vs. ExPASy Swiss-Prot
Match: Q8VYZ0 (E3 ubiquitin-protein ligase ORTHRUS 2 OS=Arabidopsis thaliana OX=3702 GN=ORTH2 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.3e-42
Identity = 96/160 (60.00%), Postives = 110/160 (68.75%), Query Frame = 0


Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           MA+  QLPCDGDG+CM C++ P   E++TC TCVTPWHV CLS+PP+TLASTLQW CPDC
Sbjct: 1   MARDIQLPCDGDGVCMRCKSNPPPEESLTCGTCVTPWHVSCLSSPPKTLASTLQWHCPDC 60

Query: 61  SPPPEDAPLPPPGNCPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S   E  PLP  G      +  SDLVAAIRAIEAD SL+  EKAK RQ LLSGK   E D
Sbjct: 61  S--GEIDPLPVSGGATGFESAGSDLVAAIRAIEADESLSTEEKAKMRQRLLSGKGVEEDD 120

Query: 121 GHDANKEKRKRGD---DVLDLFDERLNCSFCMQLPERPVT 158
             +  K+K K  +   DVL    + L CSFCMQLPERPVT
Sbjct: 121 EEEKRKKKGKGKNPNLDVLSALGDNLMCSFCMQLPERPVT 158

[Close]

BLAST of Lag0033544 vs. ExPASy Swiss-Prot
Match: Q9FVS2 (E3 ubiquitin-protein ligase ORTHRUS 3 OS=Arabidopsis thaliana OX=3702 GN=ORTH3 PE=3 SV=2)

HSP 1 Score: 144.8 bits (364), Expect = 8.6e-34
Identity = 81/159 (50.94%), Postives = 106/159 (66.67%), Query Frame = 0


Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLASTLQWDCPDC 60
           M   +Q PCD +G+CM C++ P   E++TC TCVTPWHV CL +PPETL++TLQW CPDC
Sbjct: 1   MTPATQYPCDPEGVCMRCKSMPPPEESLTCGTCVTPWHVSCLLSPPETLSATLQWLCPDC 60

Query: 61  SPPPEDAPLPPPGNCPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEKD 120
           S   E  PLP  G      +  SDLVAAI +IEAD +L+  EKAKK+Q+LLSGK   ++D
Sbjct: 61  S--GETNPLPVSGVAAGYGSVGSDLVAAIHSIEADETLSAEEKAKKKQQLLSGKGVVDED 120

Query: 121 GHDANKE--KRKRGDDVLDLFDERLNCSFCMQLPERPVT 158
             +  K+  K K+  DVL  F+    CSFCMQ  ++PV+
Sbjct: 121 DEEEKKKTSKGKKPIDVLSHFE----CSFCMQSLQKPVS 153

[Close]

BLAST of Lag0033544 vs. ExPASy Swiss-Prot
Match: Q9FKA7 (E3 ubiquitin-protein ligase ORTHRUS 1 OS=Arabidopsis thaliana OX=3702 GN=ORTH1 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.1e-32
Identity = 84/158 (53.16%), Postives = 104/158 (65.82%), Query Frame = 0


Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLA-STLQWDCPD 60
           MA  +QLPCDGDG+CM CQ  P   ET+TC TCVTPWHV CL   PE+LA ST +W+CPD
Sbjct: 1   MAIETQLPCDGDGVCMRCQVNPPSEETLTCGTCVTPWHVPCLL--PESLASSTGEWECPD 60

Query: 61  CSPPPEDAPLPPPGNCPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEK 120
           CS     +  P  GN   P +  S LVAAIRAI+AD +LT+ EKAKKRQ+L+SG      
Sbjct: 61  CSGVVVPSAAPGTGNA-RPESSGSVLVAAIRAIQADETLTEAEKAKKRQKLMSGGGD--- 120

Query: 121 DGHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVT 158
           DG D  ++K+      L++F     CS C+QLPERP+T
Sbjct: 121 DGVDEEEKKK------LEIF-----CSICIQLPERPIT 141

[Close]

BLAST of Lag0033544 vs. ExPASy Swiss-Prot
Match: Q9C8E1 (Putative E3 ubiquitin-protein ligase ORTHRUS 4 OS=Arabidopsis thaliana OX=3702 GN=ORTH4 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 8.9e-31
Identity = 86/158 (54.43%), Postives = 103/158 (65.19%), Query Frame = 0


Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLA-STLQWDCPD 60
           MA  +QLPCDGDG+CM CQ  P   ET+TC TCVTPWHV CL   PE+LA ST  W+CPD
Sbjct: 1   MAIQTQLPCDGDGVCMRCQVTPPSEETLTCGTCVTPWHVSCLL--PESLASSTGDWECPD 60

Query: 61  CSPPPEDAPLPPPGNCPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEK 120
           CS     +  P  G    P +  S LVAAIRAI+AD +LT+ EKAKKRQ L+SG      
Sbjct: 61  CSGVVVPSAAPGTG-ISGPESSGSVLVAAIRAIQADVTLTEAEKAKKRQRLMSGGGD--- 120

Query: 121 DGHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVT 158
           DG D ++EK+K     L++F     CS C+QLPERPVT
Sbjct: 121 DGVD-DEEKKK-----LEIF-----CSICIQLPERPVT 141

[Close]

BLAST of Lag0033544 vs. ExPASy Swiss-Prot
Match: Q680I0 (E3 ubiquitin-protein ligase ORTHRUS 5 OS=Arabidopsis thaliana OX=3702 GN=ORTH5 PE=2 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.0e-30
Identity = 85/158 (53.80%), Postives = 102/158 (64.56%), Query Frame = 0


Query: 1   MAQVSQLPCDGDGICMLCQAKPSDLETITCKTCVTPWHVGCLSNPPETLA-STLQWDCPD 60
           MA  +QLPCDGDG+CM CQ  P   ET+TC TCVTPWHV CL   PE+LA ST  W+CPD
Sbjct: 1   MAIQTQLPCDGDGVCMRCQVNPPSEETLTCGTCVTPWHVSCLL--PESLASSTGDWECPD 60

Query: 61  CSPPPEDAPLPPPGNCPLPTAPSSDLVAAIRAIEADASLTDREKAKKRQELLSGKSQSEK 120
           CS     +  P  G    P +  S LV AIRAI+AD +LT+ EKAKKRQ L+SG      
Sbjct: 61  CSGVVVPSAAPGTG-ISGPESSGSVLVTAIRAIQADVTLTEAEKAKKRQRLMSGGGD--- 120

Query: 121 DGHDANKEKRKRGDDVLDLFDERLNCSFCMQLPERPVT 158
           DG D ++EK+K     L++F     CS C+QLPERPVT
Sbjct: 121 DGVD-DEEKKK-----LEIF-----CSICIQLPERPVT 141

The following BLAST results are available for this feature:

BLAST of Lag0033544 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022933744.1	2.4e-73	89.81	E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita moschata]	[more]
XP_023531327.1	2.4e-73	89.81	E3 ubiquitin-protein ligase ORTHRUS 2-like [Cucurbita pepo subsp. pepo]	[more]
KAG6587385.1	2.4e-73	89.81	E3 ubiquitin-protein ligase ORTHRUS 2, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022994127.1	5.3e-73	88.54	E3 ubiquitin-protein ligase ORTHRUS 2-like isoform X2 [Cucurbita maxima]	[more]
XP_022994113.1	5.3e-73	88.54	E3 ubiquitin-protein ligase ORTHRUS 2-like isoform X1 [Cucurbita maxima] >XP_022...	[more]

BLAST of Lag0033544 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYZ0	1.3e-42	60.00	E3 ubiquitin-protein ligase ORTHRUS 2 OS=Arabidopsis thaliana OX=3702 GN=ORTH2 P...	[more]
Q9FVS2	8.6e-34	50.94	E3 ubiquitin-protein ligase ORTHRUS 3 OS=Arabidopsis thaliana OX=3702 GN=ORTH3 P...	[more]
Q9FKA7	2.1e-32	53.16	E3 ubiquitin-protein ligase ORTHRUS 1 OS=Arabidopsis thaliana OX=3702 GN=ORTH1 P...	[more]
Q9C8E1	8.9e-31	54.43	Putative E3 ubiquitin-protein ligase ORTHRUS 4 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q680I0	2.0e-30	53.80	E3 ubiquitin-protein ligase ORTHRUS 5 OS=Arabidopsis thaliana OX=3702 GN=ORTH5 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001965	Zinc finger, PHD-type	SMART	SM00249	PHD_3	coord: 14..61 e-value: 6.0E-5 score: 32.5
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 2..69 e-value: 3.2E-7 score: 31.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 61..78
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 60..80
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 98..133
None	No IPR available	PANTHER	PTHR14140:SF42	FINGER PROTEIN, PUTATIVE-RELATED	coord: 1..157
IPR045134	UHRF1/2-like	PANTHER	PTHR14140	E3 UBIQUITIN-PROTEIN LIGASE UHRF-RELATED	coord: 1..157
IPR019786	Zinc finger, PHD-type, conserved site	PROSITE	PS01359	ZF_PHD_1	coord: 15..60
IPR019787	Zinc finger, PHD-finger	PROSITE	PS50016	ZF_PHD_2	coord: 12..63 score: 8.5644
IPR011011	Zinc finger, FYVE/PHD-type	SUPERFAMILY	57903	FYVE/PHD zinc finger	coord: 7..67

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0033544.1	Lag0033544.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006325	chromatin organization
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity