Lag0033326 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033326
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionF-box/kelch-repeat protein SKIP30
Locationchr11: 42775955 .. 42776992 (+)
RNA-Seq ExpressionLag0033326
SyntenyLag0033326
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGAATTGATTGACGGTCTCCCAGACGCTGTTGCCCTCAGGTGCCTTGCACGTGTTCCCTTCCACCTCTATCCAAAGTTGGAGCTTGTTTGTCGCTCCTGGAAAGCTGCCATTCGTAGTGCTGAACTATTCAGAGTTCGACAGGAGATTGGATCATCAGAAGATCTATTATGCGTGTGTGCATTTGAACCTGAGAATCTATGGCAGCTTTATGACCCCCTCAGAGACCTTTGGTTAACAATTCCAGTACTTCCATCGAGAATTAGGCATCTTGCACACTTCGGTGCTGTCTCAACTGCTGGAAGGTTGTTTGTGCTTGGTGGTGGCAGTGATGCTGTTGATCCATTAACTGGTGATCAAGATAGGAACTTTGCAACCAATGAGGTGTGGTCATATGATCCTGTAATACGTGGGTGGTCTCAACGTGCACCGATGCTTGTACCACGTGCCATGTTTGCTTGCTGTGTTTTGGGTGGAAAGATTGTTGTTGCGGGTGGTTTTACTAGCTGCAGAAAATCAATTTCCCAGGCAGAAATGTACGATCCTGATGAAAATGTTTGGAGTCCACTGCCTGATCTACATCATACTCACAATTCAGCTTGCACAGGGGTAGTGATTGGAGGGAGGTTGCACGTCTTGCACAAGGGATTATCAACAGTGCAAATTTTCGACAATGTGCGGCCTGGATGGAGAGTTGAGGATTATGGTTGGCTCCAAGGTCCAATGGCCGTTGTCCAAGGTTCACTTTATGTGATGAGTCATGGACTCATTTTCAGGCACGATGGAGAAGTGAGGAAGGTTGTTATTTCAGCATCCGAGTTTCGGCAACGAATTGGATATGCAATGATCAGTTTGAGAGATGAAATTTATGTGATTGGAGGAGATATTGGTCCCGACCGTTTGAATTGGGACGTCAAGCCAACGTCTGATGTTGACATATTGACTACAGGAGGTGAGAGGCCAACATGGCGACATGCAGCTTCAATGACAAGATGCCGTGGGGCAATTCGAGGATGTGCTCAGTTGAGAATTTAG

mRNA sequence

ATGTCTGAATTGATTGACGGTCTCCCAGACGCTGTTGCCCTCAGGTGCCTTGCACGTGTTCCCTTCCACCTCTATCCAAAGTTGGAGCTTGTTTGTCGCTCCTGGAAAGCTGCCATTCGTAGTGCTGAACTATTCAGAGTTCGACAGGAGATTGGATCATCAGAAGATCTATTATGCGTGTGTGCATTTGAACCTGAGAATCTATGGCAGCTTTATGACCCCCTCAGAGACCTTTGGTTAACAATTCCAGTACTTCCATCGAGAATTAGGCATCTTGCACACTTCGGTGCTGTCTCAACTGCTGGAAGGTTGTTTGTGCTTGGTGGTGGCAGTGATGCTGTTGATCCATTAACTGGTGATCAAGATAGGAACTTTGCAACCAATGAGGTGTGGTCATATGATCCTGTAATACGTGGGTGGTCTCAACGTGCACCGATGCTTGTACCACGTGCCATGTTTGCTTGCTGTGTTTTGGGTGGAAAGATTGTTGTTGCGGGTGGTTTTACTAGCTGCAGAAAATCAATTTCCCAGGCAGAAATGTACGATCCTGATGAAAATGTTTGGAGTCCACTGCCTGATCTACATCATACTCACAATTCAGCTTGCACAGGGGTAGTGATTGGAGGGAGGTTGCACGTCTTGCACAAGGGATTATCAACAGTGCAAATTTTCGACAATGTGCGGCCTGGATGGAGAGTTGAGGATTATGGTTGGCTCCAAGGTCCAATGGCCGTTGTCCAAGGTTCACTTTATGTGATGAGTCATGGACTCATTTTCAGGCACGATGGAGAAGTGAGGAAGGTTGTTATTTCAGCATCCGAGTTTCGGCAACGAATTGGATATGCAATGATCAGTTTGAGAGATGAAATTTATGTGATTGGAGGAGATATTGGTCCCGACCGTTTGAATTGGGACGTCAAGCCAACGTCTGATGTTGACATATTGACTACAGGAGGTGAGAGGCCAACATGGCGACATGCAGCTTCAATGACAAGATGCCGTGGGGCAATTCGAGGATGTGCTCAGTTGAGAATTTAG

Coding sequence (CDS)

ATGTCTGAATTGATTGACGGTCTCCCAGACGCTGTTGCCCTCAGGTGCCTTGCACGTGTTCCCTTCCACCTCTATCCAAAGTTGGAGCTTGTTTGTCGCTCCTGGAAAGCTGCCATTCGTAGTGCTGAACTATTCAGAGTTCGACAGGAGATTGGATCATCAGAAGATCTATTATGCGTGTGTGCATTTGAACCTGAGAATCTATGGCAGCTTTATGACCCCCTCAGAGACCTTTGGTTAACAATTCCAGTACTTCCATCGAGAATTAGGCATCTTGCACACTTCGGTGCTGTCTCAACTGCTGGAAGGTTGTTTGTGCTTGGTGGTGGCAGTGATGCTGTTGATCCATTAACTGGTGATCAAGATAGGAACTTTGCAACCAATGAGGTGTGGTCATATGATCCTGTAATACGTGGGTGGTCTCAACGTGCACCGATGCTTGTACCACGTGCCATGTTTGCTTGCTGTGTTTTGGGTGGAAAGATTGTTGTTGCGGGTGGTTTTACTAGCTGCAGAAAATCAATTTCCCAGGCAGAAATGTACGATCCTGATGAAAATGTTTGGAGTCCACTGCCTGATCTACATCATACTCACAATTCAGCTTGCACAGGGGTAGTGATTGGAGGGAGGTTGCACGTCTTGCACAAGGGATTATCAACAGTGCAAATTTTCGACAATGTGCGGCCTGGATGGAGAGTTGAGGATTATGGTTGGCTCCAAGGTCCAATGGCCGTTGTCCAAGGTTCACTTTATGTGATGAGTCATGGACTCATTTTCAGGCACGATGGAGAAGTGAGGAAGGTTGTTATTTCAGCATCCGAGTTTCGGCAACGAATTGGATATGCAATGATCAGTTTGAGAGATGAAATTTATGTGATTGGAGGAGATATTGGTCCCGACCGTTTGAATTGGGACGTCAAGCCAACGTCTGATGTTGACATATTGACTACAGGAGGTGAGAGGCCAACATGGCGACATGCAGCTTCAATGACAAGATGCCGTGGGGCAATTCGAGGATGTGCTCAGTTGAGAATTTAG

Protein sequence

MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCVCAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGDQDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEMYDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQGPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPDRLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI
Homology
BLAST of Lag0033326 vs. NCBI nr
Match: XP_023511586.1 (F-box/kelch-repeat protein SKIP30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 671.8 bits (1732), Expect = 3.2e-189
Identity = 316/345 (91.59%), Postives = 332/345 (96.23%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MSELIDGLPDAVALRCLARVPFHLYPKLELV +SW AAIRS+ELFRVRQEIGSSEDLLCV
Sbjct: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVSKSWLAAIRSSELFRVRQEIGSSEDLLCV 60

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAFEPENLWQLYDPLRDLW+TIPVLPSRIRHLAHFGAVSTAG+LFVLGGGSD VDPLTGD
Sbjct: 61  CAFEPENLWQLYDPLRDLWITIPVLPSRIRHLAHFGAVSTAGKLFVLGGGSDDVDPLTGD 120

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
           QDRNFATNEVWSYDP+I  W QRA MLVPRAMFACCVLGG+IVVAGGFTSCRKSISQAEM
Sbjct: 121 QDRNFATNEVWSYDPIIHRWFQRASMLVPRAMFACCVLGGRIVVAGGFTSCRKSISQAEM 180

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDPD++VW PLPDLHHTHNSACTGVVIGG+LHVLHKGLSTVQIFD+V+  WRVEDYGWLQ
Sbjct: 181 YDPDKDVWIPLPDLHHTHNSACTGVVIGGKLHVLHKGLSTVQIFDDVQLEWRVEDYGWLQ 240

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPMAVVQGSLYVMSHG IF+HDGEVRKVVISASEFRQRIG+AMI LRDEIYVIGGDIGPD
Sbjct: 241 GPMAVVQGSLYVMSHGHIFKHDGEVRKVVISASEFRQRIGFAMIGLRDEIYVIGGDIGPD 300

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWD+KPTSDVDILTTGGERPTWRHAASMT+C GAIRGCA+LRI
Sbjct: 301 RLNWDIKPTSDVDILTTGGERPTWRHAASMTKCCGAIRGCARLRI 345

BLAST of Lag0033326 vs. NCBI nr
Match: XP_022146023.1 (F-box/kelch-repeat protein SKIP30 [Momordica charantia] >XP_022146024.1 F-box/kelch-repeat protein SKIP30 [Momordica charantia] >XP_022146025.1 F-box/kelch-repeat protein SKIP30 [Momordica charantia])

HSP 1 Score: 670.6 bits (1729), Expect = 7.1e-189
Identity = 314/345 (91.01%), Postives = 329/345 (95.36%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MSELIDGLPDAVALRCLARVPFHLY  LELV  SWKAAIRSAELFRVR+EIGSSE+LLCV
Sbjct: 1   MSELIDGLPDAVALRCLARVPFHLYQTLELVSHSWKAAIRSAELFRVRREIGSSENLLCV 60

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAFEPENLWQLYDPLRDLW+T+PV PS+IRHLAHFGAVSTAG+LFV+GGGSDAVDPLTGD
Sbjct: 61  CAFEPENLWQLYDPLRDLWITLPVFPSKIRHLAHFGAVSTAGKLFVVGGGSDAVDPLTGD 120

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
           QDRNFATNEVWSYDPVI  WSQRAPMLV RAMFACCVLGGKIVVAGGFTSCRKSISQAE+
Sbjct: 121 QDRNFATNEVWSYDPVIHKWSQRAPMLVARAMFACCVLGGKIVVAGGFTSCRKSISQAEV 180

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDPD++VW PLPDLHHTHNSACTG VIGG+LHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ
Sbjct: 181 YDPDKDVWIPLPDLHHTHNSACTGAVIGGKLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPM VVQ SLYVMSHGLIF+HD EVRKVVISASEFRQRIG+AMI LRDEIYVIGGDIGPD
Sbjct: 241 GPMVVVQDSLYVMSHGLIFKHDKEVRKVVISASEFRQRIGFAMIGLRDEIYVIGGDIGPD 300

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWD+KPTSDVDILTT GERPTWRHAA MTRCRGAIRGCA+LRI
Sbjct: 301 RLNWDIKPTSDVDILTTAGERPTWRHAAPMTRCRGAIRGCAELRI 345

BLAST of Lag0033326 vs. NCBI nr
Match: XP_022963882.1 (F-box/kelch-repeat protein SKIP30 [Cucurbita moschata] >XP_022967369.1 F-box/kelch-repeat protein SKIP30 [Cucurbita maxima] >KAG6571462.1 F-box/kelch-repeat protein SKIP30, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011226.1 F-box/kelch-repeat protein SKIP30, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 669.1 bits (1725), Expect = 2.1e-188
Identity = 315/345 (91.30%), Postives = 330/345 (95.65%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MSELIDGLPDAVALRCLARVPFHLYPKLELV +SW AAIRS+ELFRVRQEIGSSEDLLCV
Sbjct: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVSKSWLAAIRSSELFRVRQEIGSSEDLLCV 60

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAFEPENLWQLYDPLRDLW+TIPVLPSRIRHLAHFGAVSTAG+LFVLGGGSD VDPLTGD
Sbjct: 61  CAFEPENLWQLYDPLRDLWITIPVLPSRIRHLAHFGAVSTAGKLFVLGGGSDDVDPLTGD 120

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
           QDRNFATNEVWSYDP+I  W QRA MLVPRAMFACCVLGG+IVVAGGFTSCRKSISQAEM
Sbjct: 121 QDRNFATNEVWSYDPIIHRWFQRASMLVPRAMFACCVLGGRIVVAGGFTSCRKSISQAEM 180

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDPD +VW PLPDLHHTHNSACTGVVIGG+LHVLHKGLSTVQI D+V+  WRVEDYGWLQ
Sbjct: 181 YDPDRDVWIPLPDLHHTHNSACTGVVIGGKLHVLHKGLSTVQILDDVQLEWRVEDYGWLQ 240

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPMAVVQGSLYVMSHG IF+HDGEVRKVVISASEFRQRIG+AMI LRDEIYVIGGDIGPD
Sbjct: 241 GPMAVVQGSLYVMSHGHIFKHDGEVRKVVISASEFRQRIGFAMIGLRDEIYVIGGDIGPD 300

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWD+KPTSDVDILTTGGERPTWRHAASMT+C GAIRGCA+LRI
Sbjct: 301 RLNWDIKPTSDVDILTTGGERPTWRHAASMTKCCGAIRGCARLRI 345

BLAST of Lag0033326 vs. NCBI nr
Match: XP_022931065.1 (F-box/kelch-repeat protein SKIP30-like [Cucurbita moschata] >XP_022931066.1 F-box/kelch-repeat protein SKIP30-like [Cucurbita moschata] >XP_022931067.1 F-box/kelch-repeat protein SKIP30-like [Cucurbita moschata])

HSP 1 Score: 663.7 bits (1711), Expect = 8.7e-187
Identity = 312/345 (90.43%), Postives = 327/345 (94.78%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MSELID LPDAVALRCLARVPFHLYPKLELV  SW+AAI SAEL+RVRQEIGSSEDLLCV
Sbjct: 1   MSELIDRLPDAVALRCLARVPFHLYPKLELVSHSWQAAIHSAELYRVRQEIGSSEDLLCV 60

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAFEPENLWQLYDPLRDLW+TIPVLPS+IRHLA FGAVSTAG+LFVLGGGSD+VDPLTGD
Sbjct: 61  CAFEPENLWQLYDPLRDLWMTIPVLPSKIRHLARFGAVSTAGKLFVLGGGSDSVDPLTGD 120

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
           QDRNFATNEVWSYDPVIR WSQ A MLVPRAMFACCVL GKIVVAGGFTSCRKSISQAEM
Sbjct: 121 QDRNFATNEVWSYDPVIRRWSQCASMLVPRAMFACCVLDGKIVVAGGFTSCRKSISQAEM 180

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDPD++ W PLPDLHHTH+SACTGVVIGG+LHVLHKGLSTVQIF+N RPGWRVEDYGWLQ
Sbjct: 181 YDPDKDAWIPLPDLHHTHHSACTGVVIGGKLHVLHKGLSTVQIFENTRPGWRVEDYGWLQ 240

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPM VVQGSLYVMSHG IF+HDGE +KVVISASEFRQRIG+AM+SLRDEIYVIGGDIGPD
Sbjct: 241 GPMTVVQGSLYVMSHGHIFKHDGEAKKVVISASEFRQRIGFAMMSLRDEIYVIGGDIGPD 300

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWDVKPTSDVDILTTGGERPTWR A SMTRC GAIRGCAQLRI
Sbjct: 301 RLNWDVKPTSDVDILTTGGERPTWRQATSMTRCHGAIRGCAQLRI 345

BLAST of Lag0033326 vs. NCBI nr
Match: XP_022996118.1 (F-box/kelch-repeat protein SKIP30-like [Cucurbita maxima] >XP_022996119.1 F-box/kelch-repeat protein SKIP30-like [Cucurbita maxima] >XP_022996120.1 F-box/kelch-repeat protein SKIP30-like [Cucurbita maxima] >XP_022996121.1 F-box/kelch-repeat protein SKIP30-like [Cucurbita maxima])

HSP 1 Score: 661.8 bits (1706), Expect = 3.3e-186
Identity = 310/345 (89.86%), Postives = 327/345 (94.78%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MSELID LPDAVALRCLARVPFHLYP+LELV  SW+AAI SAEL+RVRQEIGSSEDLLCV
Sbjct: 1   MSELIDRLPDAVALRCLARVPFHLYPQLELVSHSWQAAIHSAELYRVRQEIGSSEDLLCV 60

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAFEPENLWQLYDPLRDLW+TIPVLPS+IRHLA FGAVSTAG+LFVLGGGSD+VDPLTGD
Sbjct: 61  CAFEPENLWQLYDPLRDLWMTIPVLPSKIRHLARFGAVSTAGKLFVLGGGSDSVDPLTGD 120

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
           QDRNFATNEVWSYDPVIR WSQ A MLVPRAMFACCVL GKIVVAGGFTSCRKSISQAEM
Sbjct: 121 QDRNFATNEVWSYDPVIRRWSQCASMLVPRAMFACCVLDGKIVVAGGFTSCRKSISQAEM 180

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDPD++ W PLPDLHHTH+SACTGVVIGG+LHVLHKGLSTVQIF+N RPGWRVEDYGWLQ
Sbjct: 181 YDPDKDAWIPLPDLHHTHHSACTGVVIGGKLHVLHKGLSTVQIFENTRPGWRVEDYGWLQ 240

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPM VVQGSLYVMSHG IF+HDGE +KV+ISASEFRQRIG+AM+SLRDEIYVIGGDIGPD
Sbjct: 241 GPMTVVQGSLYVMSHGHIFKHDGEAKKVIISASEFRQRIGFAMMSLRDEIYVIGGDIGPD 300

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWDVKPTSDVDILTTGGERPTWR A SMTRC GAIRGCAQLRI
Sbjct: 301 RLNWDVKPTSDVDILTTGGERPTWRRATSMTRCHGAIRGCAQLRI 345

BLAST of Lag0033326 vs. ExPASy Swiss-Prot
Match: Q9M1W7 (F-box/kelch-repeat protein SKIP30 OS=Arabidopsis thaliana OX=3702 GN=SKIP30 PE=1 SV=2)

HSP 1 Score: 535.4 bits (1378), Expect = 4.7e-151
Identity = 244/345 (70.72%), Postives = 289/345 (83.77%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MS L+DG+P+AVALRCLA VP HL+P LELV RSW+AAIRS ELFRVR+E+ SSE LLCV
Sbjct: 8   MSGLLDGIPEAVALRCLAHVPLHLHPNLELVSRSWRAAIRSHELFRVRKELRSSEHLLCV 67

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAF+PEN+WQ+Y P  D WLT+P+LPSRIRHLAHFGAV+TAG LFVLGGGSDAV P+TGD
Sbjct: 68  CAFDPENIWQVYSPNCDRWLTLPLLPSRIRHLAHFGAVTTAGMLFVLGGGSDAVSPVTGD 127

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
            D  FAT++VWSYD V R W+ RA MLVPRAMFACCVL GKIVVAGGFT+CRKSIS AEM
Sbjct: 128 HDGTFATDQVWSYDFVQRQWTPRASMLVPRAMFACCVLQGKIVVAGGFTTCRKSISGAEM 187

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDP+ +VW+ +PDLH THNSAC+G+V+ G++HVLHKGLSTVQ+ ++V+ GW V+DYGW Q
Sbjct: 188 YDPENDVWTSIPDLHQTHNSACSGLVVNGKVHVLHKGLSTVQVLESVKLGWDVKDYGWPQ 247

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPM VV+  LYVMSHGL+F+ +G+  K+V SASEF++RIG AM SL DE+ ++GG IGPD
Sbjct: 248 GPMVVVEDVLYVMSHGLVFKQEGDTWKMVASASEFKRRIGMAMTSLSDEVLIVGGVIGPD 307

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWD+KP SDVD LT G +RP WR  A MTRCRG I GC QL I
Sbjct: 308 RLNWDIKPLSDVDALTVGNDRPAWRSVAPMTRCRGTILGCTQLTI 352

BLAST of Lag0033326 vs. ExPASy Swiss-Prot
Match: Q0WW40 (F-box/kelch-repeat protein At1g16250 OS=Arabidopsis thaliana OX=3702 GN=At1g16250 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.3e-28
Identity = 108/380 (28.42%), Postives = 161/380 (42.37%), Query Frame = 0

Query: 4   LIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCVCAF 63
           +I GLPD +ALRC+A++    +  LE V R W+  +R A+    +   G S   L V   
Sbjct: 8   IIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLFVLTE 67

Query: 64  EPENLWQLYDPLRDLWLTIPVLPSRIR------HLAHFGAVSTAGRLFVLGGGSDAVDPL 123
             +N W  YDP  D W  +P    R R      H + F  V  +  L V+ GG  A    
Sbjct: 68  RSKNQWVAYDPEADRWHPLP----RTRAVQDGWHHSGFACVCVSNCLLVI-GGCYAPSVS 127

Query: 124 TGDQDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFT-SCRKSIS 183
           +    +   T +V  +DP  + W   A M  PR  FAC  + GK+ VAGG   +  + I 
Sbjct: 128 SFPHQKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYVAGGRNLTHSRGIP 187

Query: 184 QAEMYDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGL-----STVQIFDNVRPGW 243
            AE+YDP  + W  LP +       C+G+   G  HVL   +     ++ ++F+     W
Sbjct: 188 SAEVYDPVADRWEELPAMPRPQMD-CSGLSYRGCFHVLSDQVGFAEQNSSEVFNPRDMTW 247

Query: 244 RVEDYGW-----LQGPMAVVQG----SLYVMSHGLIFRHD---------GEVRKVVI-SA 303
              +  W     +Q  + V++     ++      LI   D         G V  VV+ + 
Sbjct: 248 STVEDVWPFSRAMQFAVQVMKNDRVYTIVDWGESLIKTRDTDEGEWYNVGSVPSVVLPNH 307

Query: 304 SEFRQRIGYAMISLRDEIYVIGGDIGPDRLNWDVKPTSDVDI-------LTTGGERP-TW 344
               +  GY   +LR+E+YVIGG +    L W+       DI       +    +RP  W
Sbjct: 308 PRELEAFGYGFAALRNELYVIGGKV----LKWEESGAGRFDIVRLPVVRVCNPLDRPLNW 367

BLAST of Lag0033326 vs. ExPASy Swiss-Prot
Match: Q93W93 (F-box/kelch-repeat protein At1g55270 OS=Arabidopsis thaliana OX=3702 GN=At1g55270 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.2e-23
Identity = 79/259 (30.50%), Postives = 116/259 (44.79%), Query Frame = 0

Query: 4   LIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCVCAF 63
           L+ GLPD +A+ CL RVP   + KL LVC+ W         +  R+ +G SE+ + V   
Sbjct: 78  LLPGLPDDLAVACLIRVPRAEHRKLRLVCKRWYRLASGNFFYSQRKLLGMSEEWVYVFKR 137

Query: 64  EPEN--LWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGDQ 123
           + +    W  +DP+  LW  +P +P        FG    +G    L GG    DPL G  
Sbjct: 138 DRDGKISWNTFDPISQLWQPLPPVPREYSEAVGFGCAVLSGCHLYLFGGK---DPLRGSM 197

Query: 124 DRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGG-FTSCRKSISQAEM 183
            R      V  Y+     W +   ML  R  F CCV+   + VAGG     ++++  AE+
Sbjct: 198 RR------VIFYNARTNKWHRAPDMLRKRHFFGCCVINNCLYVAGGECEGIQRTLRSAEV 257

Query: 184 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQI-----FDNVRPGWRVED 243
           YDP++N WS + D+  T      GVV   +  +  KGL + Q+     +D     W    
Sbjct: 258 YDPNKNRWSFIADM-STAMVPLIGVVYDKKWFL--KGLGSHQLVMSEAYDPEVNSWSPVS 317

Query: 244 YGWLQG---PMAVVQGSLY 252
            G + G   P   + G LY
Sbjct: 318 DGMVAGWRNPCTSLNGRLY 324

BLAST of Lag0033326 vs. ExPASy Swiss-Prot
Match: Q9CAG8 (F-box/kelch-repeat protein At1g67480 OS=Arabidopsis thaliana OX=3702 GN=At1g67480 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 8.3e-23
Identity = 67/214 (31.31%), Postives = 102/214 (47.66%), Query Frame = 0

Query: 4   LIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCVCAF 63
           LI GLPD VA +CLA VP   +P +  VC+ W+  ++S E   VR+  G  E+ L V   
Sbjct: 39  LIPGLPDDVAKQCLALVPRARFPSMGSVCKKWRFVVQSKEFITVRRLAGMLEEWLYVLTM 98

Query: 64  E---PENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 123
                +N W++ D L     ++P +P   +    F  V   G+L V+ G       L   
Sbjct: 99  NAGGKDNRWEVMDCLGQKLSSLPPMPGPAK--TGFKVVVVDGKLLVIAGCCMINGSLV-- 158

Query: 124 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 183
                A+ +V+ YD  +  WS+ A + V R  FAC  + G + V GG     +S+S AE+
Sbjct: 159 -----ASADVYQYDTCLNSWSRLADLEVARYDFACAEVNGHVYVVGGHGVDGESLSSAEV 218

Query: 184 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVL 215
           YDP+   W+ +  L       C      G+L+V+
Sbjct: 219 YDPETCTWTFIESLRRP-RWGCFASAFNGKLYVM 242

BLAST of Lag0033326 vs. ExPASy Swiss-Prot
Match: Q9LM55 (F-box/kelch-repeat protein At1g22040 OS=Arabidopsis thaliana OX=3702 GN=At1g22040 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 4.6e-21
Identity = 65/223 (29.15%), Postives = 108/223 (48.43%), Query Frame = 0

Query: 4   LIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCVC-- 63
           LI  LPD ++++ LAR+P   Y  + LV R W++A+ ++E++ +R+E+G +E+ L V   
Sbjct: 43  LIPSLPDELSIQILARLPRICYSSVRLVSRRWRSAVSTSEVYSLRKELGRTEEWLYVLTK 102

Query: 64  AFEPENLWQLYDPLRDLWLTIPVLP----------------SRIRHLAHFGAV------- 123
             E + LW   DP+   W  +P +P                + I    + GA+       
Sbjct: 103 GHEDKLLWYALDPVSTKWQRLPPMPVVVYEEESRKSLSGLWNMITPSFNVGAIVRSFLGR 162

Query: 124 -STAGRLFVLGGGSDAVDP---LTGDQDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFA 183
             ++ ++   G    AVD    + G   R+   + VW +DP++  WS+ + ML  RA   
Sbjct: 163 RDSSEQMPFCGCAIGAVDGGLYVIGGLSRSKTVSCVWRFDPILNSWSEVSSMLASRAYSK 222

Query: 184 CCVLGGKIVVAGGFTSCRKSIS---QAEMYDPDENVWSPLPDL 195
             VL  K+ V GG    R  +S    AE+YDP  + WS +P +
Sbjct: 223 TGVLNKKLYVVGGVDRGRGGLSPLQSAEVYDPSTDAWSEVPSM 265

BLAST of Lag0033326 vs. ExPASy TrEMBL
Match: A0A6J1CW43 (F-box/kelch-repeat protein SKIP30 OS=Momordica charantia OX=3673 GN=LOC111015334 PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 3.5e-189
Identity = 314/345 (91.01%), Postives = 329/345 (95.36%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MSELIDGLPDAVALRCLARVPFHLY  LELV  SWKAAIRSAELFRVR+EIGSSE+LLCV
Sbjct: 1   MSELIDGLPDAVALRCLARVPFHLYQTLELVSHSWKAAIRSAELFRVRREIGSSENLLCV 60

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAFEPENLWQLYDPLRDLW+T+PV PS+IRHLAHFGAVSTAG+LFV+GGGSDAVDPLTGD
Sbjct: 61  CAFEPENLWQLYDPLRDLWITLPVFPSKIRHLAHFGAVSTAGKLFVVGGGSDAVDPLTGD 120

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
           QDRNFATNEVWSYDPVI  WSQRAPMLV RAMFACCVLGGKIVVAGGFTSCRKSISQAE+
Sbjct: 121 QDRNFATNEVWSYDPVIHKWSQRAPMLVARAMFACCVLGGKIVVAGGFTSCRKSISQAEV 180

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDPD++VW PLPDLHHTHNSACTG VIGG+LHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ
Sbjct: 181 YDPDKDVWIPLPDLHHTHNSACTGAVIGGKLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPM VVQ SLYVMSHGLIF+HD EVRKVVISASEFRQRIG+AMI LRDEIYVIGGDIGPD
Sbjct: 241 GPMVVVQDSLYVMSHGLIFKHDKEVRKVVISASEFRQRIGFAMIGLRDEIYVIGGDIGPD 300

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWD+KPTSDVDILTT GERPTWRHAA MTRCRGAIRGCA+LRI
Sbjct: 301 RLNWDIKPTSDVDILTTAGERPTWRHAAPMTRCRGAIRGCAELRI 345

BLAST of Lag0033326 vs. ExPASy TrEMBL
Match: A0A6J1HWH7 (F-box/kelch-repeat protein SKIP30 OS=Cucurbita maxima OX=3661 GN=LOC111466917 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.0e-188
Identity = 315/345 (91.30%), Postives = 330/345 (95.65%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MSELIDGLPDAVALRCLARVPFHLYPKLELV +SW AAIRS+ELFRVRQEIGSSEDLLCV
Sbjct: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVSKSWLAAIRSSELFRVRQEIGSSEDLLCV 60

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAFEPENLWQLYDPLRDLW+TIPVLPSRIRHLAHFGAVSTAG+LFVLGGGSD VDPLTGD
Sbjct: 61  CAFEPENLWQLYDPLRDLWITIPVLPSRIRHLAHFGAVSTAGKLFVLGGGSDDVDPLTGD 120

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
           QDRNFATNEVWSYDP+I  W QRA MLVPRAMFACCVLGG+IVVAGGFTSCRKSISQAEM
Sbjct: 121 QDRNFATNEVWSYDPIIHRWFQRASMLVPRAMFACCVLGGRIVVAGGFTSCRKSISQAEM 180

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDPD +VW PLPDLHHTHNSACTGVVIGG+LHVLHKGLSTVQI D+V+  WRVEDYGWLQ
Sbjct: 181 YDPDRDVWIPLPDLHHTHNSACTGVVIGGKLHVLHKGLSTVQILDDVQLEWRVEDYGWLQ 240

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPMAVVQGSLYVMSHG IF+HDGEVRKVVISASEFRQRIG+AMI LRDEIYVIGGDIGPD
Sbjct: 241 GPMAVVQGSLYVMSHGHIFKHDGEVRKVVISASEFRQRIGFAMIGLRDEIYVIGGDIGPD 300

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWD+KPTSDVDILTTGGERPTWRHAASMT+C GAIRGCA+LRI
Sbjct: 301 RLNWDIKPTSDVDILTTGGERPTWRHAASMTKCCGAIRGCARLRI 345

BLAST of Lag0033326 vs. ExPASy TrEMBL
Match: A0A6J1HGE3 (F-box/kelch-repeat protein SKIP30 OS=Cucurbita moschata OX=3662 GN=LOC111464058 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.0e-188
Identity = 315/345 (91.30%), Postives = 330/345 (95.65%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MSELIDGLPDAVALRCLARVPFHLYPKLELV +SW AAIRS+ELFRVRQEIGSSEDLLCV
Sbjct: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVSKSWLAAIRSSELFRVRQEIGSSEDLLCV 60

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAFEPENLWQLYDPLRDLW+TIPVLPSRIRHLAHFGAVSTAG+LFVLGGGSD VDPLTGD
Sbjct: 61  CAFEPENLWQLYDPLRDLWITIPVLPSRIRHLAHFGAVSTAGKLFVLGGGSDDVDPLTGD 120

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
           QDRNFATNEVWSYDP+I  W QRA MLVPRAMFACCVLGG+IVVAGGFTSCRKSISQAEM
Sbjct: 121 QDRNFATNEVWSYDPIIHRWFQRASMLVPRAMFACCVLGGRIVVAGGFTSCRKSISQAEM 180

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDPD +VW PLPDLHHTHNSACTGVVIGG+LHVLHKGLSTVQI D+V+  WRVEDYGWLQ
Sbjct: 181 YDPDRDVWIPLPDLHHTHNSACTGVVIGGKLHVLHKGLSTVQILDDVQLEWRVEDYGWLQ 240

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPMAVVQGSLYVMSHG IF+HDGEVRKVVISASEFRQRIG+AMI LRDEIYVIGGDIGPD
Sbjct: 241 GPMAVVQGSLYVMSHGHIFKHDGEVRKVVISASEFRQRIGFAMIGLRDEIYVIGGDIGPD 300

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWD+KPTSDVDILTTGGERPTWRHAASMT+C GAIRGCA+LRI
Sbjct: 301 RLNWDIKPTSDVDILTTGGERPTWRHAASMTKCCGAIRGCARLRI 345

BLAST of Lag0033326 vs. ExPASy TrEMBL
Match: A0A6J1ESG5 (F-box/kelch-repeat protein SKIP30-like OS=Cucurbita moschata OX=3662 GN=LOC111437365 PE=4 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 4.2e-187
Identity = 312/345 (90.43%), Postives = 327/345 (94.78%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MSELID LPDAVALRCLARVPFHLYPKLELV  SW+AAI SAEL+RVRQEIGSSEDLLCV
Sbjct: 1   MSELIDRLPDAVALRCLARVPFHLYPKLELVSHSWQAAIHSAELYRVRQEIGSSEDLLCV 60

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAFEPENLWQLYDPLRDLW+TIPVLPS+IRHLA FGAVSTAG+LFVLGGGSD+VDPLTGD
Sbjct: 61  CAFEPENLWQLYDPLRDLWMTIPVLPSKIRHLARFGAVSTAGKLFVLGGGSDSVDPLTGD 120

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
           QDRNFATNEVWSYDPVIR WSQ A MLVPRAMFACCVL GKIVVAGGFTSCRKSISQAEM
Sbjct: 121 QDRNFATNEVWSYDPVIRRWSQCASMLVPRAMFACCVLDGKIVVAGGFTSCRKSISQAEM 180

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDPD++ W PLPDLHHTH+SACTGVVIGG+LHVLHKGLSTVQIF+N RPGWRVEDYGWLQ
Sbjct: 181 YDPDKDAWIPLPDLHHTHHSACTGVVIGGKLHVLHKGLSTVQIFENTRPGWRVEDYGWLQ 240

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPM VVQGSLYVMSHG IF+HDGE +KVVISASEFRQRIG+AM+SLRDEIYVIGGDIGPD
Sbjct: 241 GPMTVVQGSLYVMSHGHIFKHDGEAKKVVISASEFRQRIGFAMMSLRDEIYVIGGDIGPD 300

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWDVKPTSDVDILTTGGERPTWR A SMTRC GAIRGCAQLRI
Sbjct: 301 RLNWDVKPTSDVDILTTGGERPTWRQATSMTRCHGAIRGCAQLRI 345

BLAST of Lag0033326 vs. ExPASy TrEMBL
Match: A0A6J1K7U2 (F-box/kelch-repeat protein SKIP30-like OS=Cucurbita maxima OX=3661 GN=LOC111491436 PE=4 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 1.6e-186
Identity = 310/345 (89.86%), Postives = 327/345 (94.78%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MSELID LPDAVALRCLARVPFHLYP+LELV  SW+AAI SAEL+RVRQEIGSSEDLLCV
Sbjct: 1   MSELIDRLPDAVALRCLARVPFHLYPQLELVSHSWQAAIHSAELYRVRQEIGSSEDLLCV 60

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAFEPENLWQLYDPLRDLW+TIPVLPS+IRHLA FGAVSTAG+LFVLGGGSD+VDPLTGD
Sbjct: 61  CAFEPENLWQLYDPLRDLWMTIPVLPSKIRHLARFGAVSTAGKLFVLGGGSDSVDPLTGD 120

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
           QDRNFATNEVWSYDPVIR WSQ A MLVPRAMFACCVL GKIVVAGGFTSCRKSISQAEM
Sbjct: 121 QDRNFATNEVWSYDPVIRRWSQCASMLVPRAMFACCVLDGKIVVAGGFTSCRKSISQAEM 180

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDPD++ W PLPDLHHTH+SACTGVVIGG+LHVLHKGLSTVQIF+N RPGWRVEDYGWLQ
Sbjct: 181 YDPDKDAWIPLPDLHHTHHSACTGVVIGGKLHVLHKGLSTVQIFENTRPGWRVEDYGWLQ 240

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPM VVQGSLYVMSHG IF+HDGE +KV+ISASEFRQRIG+AM+SLRDEIYVIGGDIGPD
Sbjct: 241 GPMTVVQGSLYVMSHGHIFKHDGEAKKVIISASEFRQRIGFAMMSLRDEIYVIGGDIGPD 300

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWDVKPTSDVDILTTGGERPTWR A SMTRC GAIRGCAQLRI
Sbjct: 301 RLNWDVKPTSDVDILTTGGERPTWRRATSMTRCHGAIRGCAQLRI 345

BLAST of Lag0033326 vs. TAIR 10
Match: AT3G63220.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 535.4 bits (1378), Expect = 3.3e-152
Identity = 244/345 (70.72%), Postives = 289/345 (83.77%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MS L+DG+P+AVALRCLA VP HL+P LELV RSW+AAIRS ELFRVR+E+ SSE LLCV
Sbjct: 1   MSGLLDGIPEAVALRCLAHVPLHLHPNLELVSRSWRAAIRSHELFRVRKELRSSEHLLCV 60

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAF+PEN+WQ+Y P  D WLT+P+LPSRIRHLAHFGAV+TAG LFVLGGGSDAV P+TGD
Sbjct: 61  CAFDPENIWQVYSPNCDRWLTLPLLPSRIRHLAHFGAVTTAGMLFVLGGGSDAVSPVTGD 120

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
            D  FAT++VWSYD V R W+ RA MLVPRAMFACCVL GKIVVAGGFT+CRKSIS AEM
Sbjct: 121 HDGTFATDQVWSYDFVQRQWTPRASMLVPRAMFACCVLQGKIVVAGGFTTCRKSISGAEM 180

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDP+ +VW+ +PDLH THNSAC+G+V+ G++HVLHKGLSTVQ+ ++V+ GW V+DYGW Q
Sbjct: 181 YDPENDVWTSIPDLHQTHNSACSGLVVNGKVHVLHKGLSTVQVLESVKLGWDVKDYGWPQ 240

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPM VV+  LYVMSHGL+F+ +G+  K+V SASEF++RIG AM SL DE+ ++GG IGPD
Sbjct: 241 GPMVVVEDVLYVMSHGLVFKQEGDTWKMVASASEFKRRIGMAMTSLSDEVLIVGGVIGPD 300

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWD+KP SDVD LT G +RP WR  A MTRCRG I GC QL I
Sbjct: 301 RLNWDIKPLSDVDALTVGNDRPAWRSVAPMTRCRGTILGCTQLTI 345

BLAST of Lag0033326 vs. TAIR 10
Match: AT3G63220.2 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 535.4 bits (1378), Expect = 3.3e-152
Identity = 244/345 (70.72%), Postives = 289/345 (83.77%), Query Frame = 0

Query: 1   MSELIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCV 60
           MS L+DG+P+AVALRCLA VP HL+P LELV RSW+AAIRS ELFRVR+E+ SSE LLCV
Sbjct: 8   MSGLLDGIPEAVALRCLAHVPLHLHPNLELVSRSWRAAIRSHELFRVRKELRSSEHLLCV 67

Query: 61  CAFEPENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 120
           CAF+PEN+WQ+Y P  D WLT+P+LPSRIRHLAHFGAV+TAG LFVLGGGSDAV P+TGD
Sbjct: 68  CAFDPENIWQVYSPNCDRWLTLPLLPSRIRHLAHFGAVTTAGMLFVLGGGSDAVSPVTGD 127

Query: 121 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 180
            D  FAT++VWSYD V R W+ RA MLVPRAMFACCVL GKIVVAGGFT+CRKSIS AEM
Sbjct: 128 HDGTFATDQVWSYDFVQRQWTPRASMLVPRAMFACCVLQGKIVVAGGFTTCRKSISGAEM 187

Query: 181 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQIFDNVRPGWRVEDYGWLQ 240
           YDP+ +VW+ +PDLH THNSAC+G+V+ G++HVLHKGLSTVQ+ ++V+ GW V+DYGW Q
Sbjct: 188 YDPENDVWTSIPDLHQTHNSACSGLVVNGKVHVLHKGLSTVQVLESVKLGWDVKDYGWPQ 247

Query: 241 GPMAVVQGSLYVMSHGLIFRHDGEVRKVVISASEFRQRIGYAMISLRDEIYVIGGDIGPD 300
           GPM VV+  LYVMSHGL+F+ +G+  K+V SASEF++RIG AM SL DE+ ++GG IGPD
Sbjct: 248 GPMVVVEDVLYVMSHGLVFKQEGDTWKMVASASEFKRRIGMAMTSLSDEVLIVGGVIGPD 307

Query: 301 RLNWDVKPTSDVDILTTGGERPTWRHAASMTRCRGAIRGCAQLRI 346
           RLNWD+KP SDVD LT G +RP WR  A MTRCRG I GC QL I
Sbjct: 308 RLNWDIKPLSDVDALTVGNDRPAWRSVAPMTRCRGTILGCTQLTI 352

BLAST of Lag0033326 vs. TAIR 10
Match: AT1G16250.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 128.6 bits (322), Expect = 9.4e-30
Identity = 108/380 (28.42%), Postives = 161/380 (42.37%), Query Frame = 0

Query: 4   LIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCVCAF 63
           +I GLPD +ALRC+A++    +  LE V R W+  +R A+    +   G S   L V   
Sbjct: 8   IIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLFVLTE 67

Query: 64  EPENLWQLYDPLRDLWLTIPVLPSRIR------HLAHFGAVSTAGRLFVLGGGSDAVDPL 123
             +N W  YDP  D W  +P    R R      H + F  V  +  L V+ GG  A    
Sbjct: 68  RSKNQWVAYDPEADRWHPLP----RTRAVQDGWHHSGFACVCVSNCLLVI-GGCYAPSVS 127

Query: 124 TGDQDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFT-SCRKSIS 183
           +    +   T +V  +DP  + W   A M  PR  FAC  + GK+ VAGG   +  + I 
Sbjct: 128 SFPHQKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYVAGGRNLTHSRGIP 187

Query: 184 QAEMYDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGL-----STVQIFDNVRPGW 243
            AE+YDP  + W  LP +       C+G+   G  HVL   +     ++ ++F+     W
Sbjct: 188 SAEVYDPVADRWEELPAMPRPQMD-CSGLSYRGCFHVLSDQVGFAEQNSSEVFNPRDMTW 247

Query: 244 RVEDYGW-----LQGPMAVVQG----SLYVMSHGLIFRHD---------GEVRKVVI-SA 303
              +  W     +Q  + V++     ++      LI   D         G V  VV+ + 
Sbjct: 248 STVEDVWPFSRAMQFAVQVMKNDRVYTIVDWGESLIKTRDTDEGEWYNVGSVPSVVLPNH 307

Query: 304 SEFRQRIGYAMISLRDEIYVIGGDIGPDRLNWDVKPTSDVDI-------LTTGGERP-TW 344
               +  GY   +LR+E+YVIGG +    L W+       DI       +    +RP  W
Sbjct: 308 PRELEAFGYGFAALRNELYVIGGKV----LKWEESGAGRFDIVRLPVVRVCNPLDRPLNW 367

BLAST of Lag0033326 vs. TAIR 10
Match: AT1G55270.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 111.3 bits (277), Expect = 1.6e-24
Identity = 79/259 (30.50%), Postives = 116/259 (44.79%), Query Frame = 0

Query: 4   LIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCVCAF 63
           L+ GLPD +A+ CL RVP   + KL LVC+ W         +  R+ +G SE+ + V   
Sbjct: 78  LLPGLPDDLAVACLIRVPRAEHRKLRLVCKRWYRLASGNFFYSQRKLLGMSEEWVYVFKR 137

Query: 64  EPEN--LWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGDQ 123
           + +    W  +DP+  LW  +P +P        FG    +G    L GG    DPL G  
Sbjct: 138 DRDGKISWNTFDPISQLWQPLPPVPREYSEAVGFGCAVLSGCHLYLFGGK---DPLRGSM 197

Query: 124 DRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGG-FTSCRKSISQAEM 183
            R      V  Y+     W +   ML  R  F CCV+   + VAGG     ++++  AE+
Sbjct: 198 RR------VIFYNARTNKWHRAPDMLRKRHFFGCCVINNCLYVAGGECEGIQRTLRSAEV 257

Query: 184 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVLHKGLSTVQI-----FDNVRPGWRVED 243
           YDP++N WS + D+  T      GVV   +  +  KGL + Q+     +D     W    
Sbjct: 258 YDPNKNRWSFIADM-STAMVPLIGVVYDKKWFL--KGLGSHQLVMSEAYDPEVNSWSPVS 317

Query: 244 YGWLQG---PMAVVQGSLY 252
            G + G   P   + G LY
Sbjct: 318 DGMVAGWRNPCTSLNGRLY 324

BLAST of Lag0033326 vs. TAIR 10
Match: AT1G67480.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 109.4 bits (272), Expect = 5.9e-24
Identity = 67/214 (31.31%), Postives = 102/214 (47.66%), Query Frame = 0

Query: 4   LIDGLPDAVALRCLARVPFHLYPKLELVCRSWKAAIRSAELFRVRQEIGSSEDLLCVCAF 63
           LI GLPD VA +CLA VP   +P +  VC+ W+  ++S E   VR+  G  E+ L V   
Sbjct: 39  LIPGLPDDVAKQCLALVPRARFPSMGSVCKKWRFVVQSKEFITVRRLAGMLEEWLYVLTM 98

Query: 64  E---PENLWQLYDPLRDLWLTIPVLPSRIRHLAHFGAVSTAGRLFVLGGGSDAVDPLTGD 123
                +N W++ D L     ++P +P   +    F  V   G+L V+ G       L   
Sbjct: 99  NAGGKDNRWEVMDCLGQKLSSLPPMPGPAK--TGFKVVVVDGKLLVIAGCCMINGSLV-- 158

Query: 124 QDRNFATNEVWSYDPVIRGWSQRAPMLVPRAMFACCVLGGKIVVAGGFTSCRKSISQAEM 183
                A+ +V+ YD  +  WS+ A + V R  FAC  + G + V GG     +S+S AE+
Sbjct: 159 -----ASADVYQYDTCLNSWSRLADLEVARYDFACAEVNGHVYVVGGHGVDGESLSSAEV 218

Query: 184 YDPDENVWSPLPDLHHTHNSACTGVVIGGRLHVL 215
           YDP+   W+ +  L       C      G+L+V+
Sbjct: 219 YDPETCTWTFIESLRRP-RWGCFASAFNGKLYVM 242

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023511586.13.2e-18991.59F-box/kelch-repeat protein SKIP30 [Cucurbita pepo subsp. pepo][more]
XP_022146023.17.1e-18991.01F-box/kelch-repeat protein SKIP30 [Momordica charantia] >XP_022146024.1 F-box/ke... [more]
XP_022963882.12.1e-18891.30F-box/kelch-repeat protein SKIP30 [Cucurbita moschata] >XP_022967369.1 F-box/kel... [more]
XP_022931065.18.7e-18790.43F-box/kelch-repeat protein SKIP30-like [Cucurbita moschata] >XP_022931066.1 F-bo... [more]
XP_022996118.13.3e-18689.86F-box/kelch-repeat protein SKIP30-like [Cucurbita maxima] >XP_022996119.1 F-box/... [more]
Match NameE-valueIdentityDescription
Q9M1W74.7e-15170.72F-box/kelch-repeat protein SKIP30 OS=Arabidopsis thaliana OX=3702 GN=SKIP30 PE=1... [more]
Q0WW401.3e-2828.42F-box/kelch-repeat protein At1g16250 OS=Arabidopsis thaliana OX=3702 GN=At1g1625... [more]
Q93W932.2e-2330.50F-box/kelch-repeat protein At1g55270 OS=Arabidopsis thaliana OX=3702 GN=At1g5527... [more]
Q9CAG88.3e-2331.31F-box/kelch-repeat protein At1g67480 OS=Arabidopsis thaliana OX=3702 GN=At1g6748... [more]
Q9LM554.6e-2129.15F-box/kelch-repeat protein At1g22040 OS=Arabidopsis thaliana OX=3702 GN=At1g2204... [more]
Match NameE-valueIdentityDescription
A0A6J1CW433.5e-18991.01F-box/kelch-repeat protein SKIP30 OS=Momordica charantia OX=3673 GN=LOC111015334... [more]
A0A6J1HWH71.0e-18891.30F-box/kelch-repeat protein SKIP30 OS=Cucurbita maxima OX=3661 GN=LOC111466917 PE... [more]
A0A6J1HGE31.0e-18891.30F-box/kelch-repeat protein SKIP30 OS=Cucurbita moschata OX=3662 GN=LOC111464058 ... [more]
A0A6J1ESG54.2e-18790.43F-box/kelch-repeat protein SKIP30-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1K7U21.6e-18689.86F-box/kelch-repeat protein SKIP30-like OS=Cucurbita maxima OX=3661 GN=LOC1114914... [more]
Match NameE-valueIdentityDescription
AT3G63220.13.3e-15270.72Galactose oxidase/kelch repeat superfamily protein [more]
AT3G63220.23.3e-15270.72Galactose oxidase/kelch repeat superfamily protein [more]
AT1G16250.19.4e-3028.42Galactose oxidase/kelch repeat superfamily protein [more]
AT1G55270.11.6e-2430.50Galactose oxidase/kelch repeat superfamily protein [more]
AT1G67480.15.9e-2431.31Galactose oxidase/kelch repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006652Kelch repeat type 1SMARTSM00612kelc_smartcoord: 103..160
e-value: 0.015
score: 24.5
coord: 161..208
e-value: 1.2E-6
score: 38.0
IPR006652Kelch repeat type 1PFAMPF01344Kelch_1coord: 278..330
e-value: 2.6E-4
score: 20.5
coord: 92..146
e-value: 5.2E-6
score: 25.9
coord: 149..194
e-value: 5.3E-13
score: 48.3
IPR001810F-box domainSMARTSM00256fbox_2coord: 8..47
e-value: 0.0088
score: 25.3
IPR001810F-box domainPFAMPF00646F-boxcoord: 5..44
e-value: 6.7E-4
score: 19.4
NoneNo IPR availableGENE3D1.20.1280.50coord: 2..57
e-value: 1.6E-5
score: 26.5
NoneNo IPR availablePANTHERPTHR24414F-BOX/KELCH-REPEAT PROTEIN SKIP4coord: 2..345
NoneNo IPR availablePANTHERPTHR24414:SF40F-BOX/KELCH-REPEAT PROTEIN SKIP30coord: 2..345
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 60..342
e-value: 1.4E-35
score: 124.7
IPR015915Kelch-type beta propellerSUPERFAMILY117281Kelch motifcoord: 61..329

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033326.1Lag0033326.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding