Lag0033274 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0033274
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	proteinase inhibitor-like
Location	chr11: 42368220 .. 42371419 (+)
RNA-Seq Expression	Lag0033274
Synteny	Lag0033274

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGGATATGAGGGTGGGAATTCAGGTATCTTCATTTATGTATAATATATATTGCATTTTTATTGGTTAAAATATTATTTTGGTCTTAGTATTTCGAAATTTGTTCTATTTTAATTCTTGTATTTCAAAAGTCCAAATTTAGTTTTAAAATTTTCAATACATCTTAAATTAGTTTCTCTAGCTTATTCTTTACTTTTGCAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCAATTACCATCTTCCATCTATAAATTTTAAAAATATATTGACATTTTGTATTTTTTTGAATGAAAATTATTATTGTTATTTTACAACTTTGATAAAAAATTAACTTGAATTTATTTTAGTTTCTTTTTTAGTTCGAATTTATTTTAGTATTTATTGATATTACAAGAACTAAATTTGGACATTTTAAACAACATGAATTAAAATGAAATGAGACTCACAAGTACCTAGACAAAAATGGGTTTTTCAAACTATTATATATGTTTGTATGTAGTCTATAAAAATTATATTTTAGTCCTTTAATGTTTTAGTCTTTTTCTAACAACTCTACAAATTTTTTAATTTTATCCTTCATATATGGATCATTTTAGTCAAGTTCTTACCCTCACTCGGACGTCAGAAAAAGACAATATATGTTGATGTCAAAATTTGGCCAAACAATCCACCTGCCCTTCACGTGACACCAACAATGAATTTGTAGAAGCTGGGGAAGACGTGACCTGCAAGACCAGCAAGAATCGCTGGACACCGGTGTGGTGTAGGCCACACCACCTCCGATGCCTAAGTCAGGAAACTGAGCAGCTAGAGAATAGAGAAAGAAGTCAAGAATCGAGTTTTCGGCCTCCTTCTTCGATGGCGAAGAGGGGTATAAATACCTGCTCGGACTCCTAGGGTTTGGAGGCGTTCTGGGACGAAGCAGGCTGGACCGAGGCAGCTGGAAGCGGTAGGGGCCGAGCGGAGGCAAACGGCCCGAGTGGTCGGCCTCGGCCCAAGGCCGAGGCCGACCGTACGGGTCGGGCCACGTTGGCCCGGCCCATCGGTATGGTCTCCCTCTGGGTCCCTTTCCTGGTCCTATCTCCTCCCGGTTGTCCCATCAGCTCCTCGTACGCTATGGTGGTCCAAAATTACCTATAACAATATATAATTTTAAAAATGAAAACTTTTTTAACATTGTCCATTGTTAGTTTAAAGGGTCAATTTTTTTTTTTTTTTTTTTTATATAACATGGAGTAATTAATTTTAAGTCAAGTAAGTCACTACATTCTAAATCGTATAAGATGGAAATTTTTTTAAATTTTAAAGGTGAGAAGTATATATCTCTCCCATCAAATAACCATTTTGTTAGGTGATTTTAATGATCAAATCATTCGTGGCTAACATATTTATATTTAGCTTAGCATATATAATATATGTATTGGTTTAAAGAAAATCATTGTTTAATTTTTTAGATGGATTGTCAACAAGAGTATGACTCAATTGACATAAAATTTGTAATTTCACCAAGAGGTTAGAGGTTCAATCCCCTATCTCAATATGTTTAAAAAAAATAGATGAATTATTGGTGTAGGAAAGAGGTTTTGGCCTGAGCTGATAGGGATATTATTGTTAAAAAAAAGTTTATTCCATACTTTTATATATATATATATATAGATAAAAATAATAAAAAAATAAAATAATAAAGGTAAAATTATGGTGGATGAAATGTGTGTAGGGGTAAGTGAGAGTATTAATACTATTAATTTGCATTATAATAGTATTAATATTAATTTTCATTTAAAAAAAGAGAGGATAATGTGTAGATATTATCTAAAAACTCAAGCTTAACATCATTTTTCAATTTTAATTTATTGAACTAAAACAAAAGATTTCTTTATTTTATTCTTGGTAGTTTGGATTCTCATTTTAGAATATATTCTTTGACCAATAAAAGAAGGGTTAAATTAAGTTATCCCAAAATCACCCTCTATATATATATATATATTTATTTTGAAGTTAACCTAAACAATTAAGTTCACAAACATAGAAGTGTGAATTCACAAGGAAAAAAGAAGATGACAGGAGATGAGGGTGGGAATTCAGGTATGCTCATATATGTATAATATATACTCCACTTTTATTTGGTTAAAATATTATTTTGGTCCTTGTGCTTTGAAATTTTATTCTATTTTAATTCTTGTATTTTCAAATTTCTGTTAGTTTATTGTTTACTTTTGAAAAAAAAAACTCAATTAGCCTTCTATCTATAAATTTTGAAAATATATTGGTATTGTATCTTTCTAGAATGAAAACTATTATTATTATTTTACAACTTTGATAAAATTTAACTTGAATTCATTTTAATATTTATTAATAGTACAAAGATTAAATTTGGACATTTTAAAGAACCAGAACTAAAATGGAACGAGGCTCTTAAATACCAGAACCAAAATGGATTTTTTCAAACTATTATATATGTTTGTATGTAGTCTATAAAATATATAATTTAGTCCTTTAAGGTTTTAATCTTTTTTTTTAACAACTCCACGATTTTTTTTTTTTTTTAATTTCATCCCTTATATATGGATCCTTTTAGTCAAGTTCTTATGATCACCCGACATTAAAAAAGGACAACATATAAATTTAAAAATGAGATCTTTTTTAATGTTGTACATTGTGAGTTTAAAAGGTCTTTTTTTTTTTTATAACAAGGAGGGATTAATTTCAAGTCAAGTCACTACATTTCTGAATAGTATAAGATAGTTCAACTGGCATAAAGTTGTACTAAAAAAAATAGTATAAGTTGGGAAAGGTTTTATACTTTATAGGTGAGAAGTATATGTCTATCCCATCAAATAATTATTTTGAAATCAGATAATTAATCACTTTGTTAGATGATTTTAATGATCAAATCATTTGTGACTAACATATTTATATTTAGCTTAGCATATATAATATGATTGGTTTAAAGAAAATCATTTTGTTAATTTTTTATATGAATTATTGGATTAGGAAAGAGGTCTTGGCCTGAGACGGTAGGGAAAGATGGAGAGGAGGCAGTCAAGATAATACAACGAGATAATCCAAAGTTGGAACCAGTTATATTACCTCCAAGCGTCAAATCCCAAACTTTGGAGTACAATTTCAAACGAGTTCGAGTCTATGTTGACGATAACTTGAAGGTGACAAGAGTTCCTGTCATTGGTTGA

mRNA sequence

ATGACAGGATATGAGGGTGGGAATTCAGAAGCTGGGGAAGACGTGACCTGCAAGACCAGCAAGAATCGCTGGACACCGGGTTTGGAGGCGTTCTGGGACGAAGCAGGCTGGACCGAGGCAGCTGGAAGCGGTAGGGGCCGAGCGGAGGCAAACGGCCCGAGTGGTCGGCCTCGGCCCAAGGCCGAGGCCGACCGTACGGGTCGGGCCACGTTGGCCCGGCCCATCGGTATGGTCTCCCTCTGGGTCCCTTTCCTGGTCCTATCTCCTCCCGGTTGTCCCATCAGCTCCTCAAGTGTGAATTCACAAGGAAAAAAGAAGATGACAGGAGATGAGGGTGGGAATTCAGGAAAGAGGTCTTGGCCTGAGACGGTAGGGAAAGATGGAGAGGAGGCAGTCAAGATAATACAACGAGATAATCCAAAGTTGGAACCAGTTATATTACCTCCAAGCGTCAAATCCCAAACTTTGGAGTACAATTTCAAACGAGTTCGAGTCTATGTTGACGATAACTTGAAGGTGACAAGAGTTCCTGTCATTGGTTGA

Coding sequence (CDS)

ATGACAGGATATGAGGGTGGGAATTCAGAAGCTGGGGAAGACGTGACCTGCAAGACCAGCAAGAATCGCTGGACACCGGGTTTGGAGGCGTTCTGGGACGAAGCAGGCTGGACCGAGGCAGCTGGAAGCGGTAGGGGCCGAGCGGAGGCAAACGGCCCGAGTGGTCGGCCTCGGCCCAAGGCCGAGGCCGACCGTACGGGTCGGGCCACGTTGGCCCGGCCCATCGGTATGGTCTCCCTCTGGGTCCCTTTCCTGGTCCTATCTCCTCCCGGTTGTCCCATCAGCTCCTCAAGTGTGAATTCACAAGGAAAAAAGAAGATGACAGGAGATGAGGGTGGGAATTCAGGAAAGAGGTCTTGGCCTGAGACGGTAGGGAAAGATGGAGAGGAGGCAGTCAAGATAATACAACGAGATAATCCAAAGTTGGAACCAGTTATATTACCTCCAAGCGTCAAATCCCAAACTTTGGAGTACAATTTCAAACGAGTTCGAGTCTATGTTGACGATAACTTGAAGGTGACAAGAGTTCCTGTCATTGGTTGA

Protein sequence

MTGYEGGNSEAGEDVTCKTSKNRWTPGLEAFWDEAGWTEAAGSGRGRAEANGPSGRPRPKAEADRTGRATLARPIGMVSLWVPFLVLSPPGCPISSSSVNSQGKKKMTGDEGGNSGKRSWPETVGKDGEEAVKIIQRDNPKLEPVILPPSVKSQTLEYNFKRVRVYVDDNLKVTRVPVIG

Homology

[Close]

BLAST of Lag0033274 vs. NCBI nr
Match: AAT72726.1 (putative chymotrypsin protease inhibitor [Cucurbita maxima])

HSP 1 Score: 73.9 bits (180), Expect = 1.5e-09
Identity = 35/64 (54.69%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 117 KRSWPETVGKDGEEAVKIIQRDNPKLEPVILPPSVKSQTLEYNFKRVRVYVDDNLKVTRV 176
           K SWPE VGKDGEEAVKIIQ++NP L+ +++P      TL+Y   RVRV+ D++ KV  +
Sbjct: 4   KSSWPELVGKDGEEAVKIIQQENPSLDVILMPRGQNWATLDYRPNRVRVFNDESGKVNSI 63

Query: 177 PVIG 181
           P IG
Sbjct: 64  PRIG 67

[Close]

BLAST of Lag0033274 vs. NCBI nr
Match: PKI46302.1 (hypothetical protein CRG98_033311 [Punica granatum])

HSP 1 Score: 73.6 bits (179), Expect = 2.0e-09
Identity = 38/67 (56.72%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 114 NSGKRSWPETVGKDGEEAVKIIQRDNPKLEPVILPPSVKSQTLEYNFKRVRVYVDDNLKV 173
           N GK SWPE VGK+G  A   I R+NP +   I+PPSV   T +Y F RVRV+VDD   V
Sbjct: 6   NPGKSSWPELVGKNGGVAAATIIRENPNVFVEIIPPSVTMITSDYGFNRVRVFVDDCCVV 65

Query: 174 TRVPVIG 181
           TRVP IG
Sbjct: 66  TRVPTIG 72

[Close]

BLAST of Lag0033274 vs. NCBI nr
Match: CAA57307.1 (Pumpkin fruit trypsin inhibitor [Cucurbita maxima])

HSP 1 Score: 72.8 bits (177), Expect = 3.4e-09
Identity = 35/64 (54.69%), Postives = 45/64 (70.31%), Query Frame = 0


Query: 117 KRSWPETVGKDGEEAVKIIQRDNPKLEPVILPPSVKSQTLEYNFKRVRVYVDDNLKVTRV 176
           K SWPE VGKDGEEAVKIIQ++NP L+ +++P      T +Y   RVRV+ DD+ KV  +
Sbjct: 4   KLSWPELVGKDGEEAVKIIQQENPSLDVILMPRGQNWATKDYRPNRVRVFNDDSGKVNSI 63

Query: 177 PVIG 181
           P IG
Sbjct: 64  PRIG 67

[Close]

BLAST of Lag0033274 vs. NCBI nr
Match: KAG6571497.1 (hypothetical protein SDJN03_28225, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 71.2 bits (173), Expect = 1.0e-08
Identity = 36/66 (54.55%), Postives = 45/66 (68.18%), Query Frame = 0


Query: 115 SGKRSWPETVGKDGEEAVKIIQRDNPKLEPVILPPSVKSQTLEYNFKRVRVYVDDNLKVT 174
           S   SWPE VGKDGEEAVKIIQ++NP L+ +++P   K  T +Y   RVRV+ DD  KV 
Sbjct: 2   SENSSWPELVGKDGEEAVKIIQQENPSLDVILMPRGQKWATRDYGPYRVRVFNDDYGKVN 61

Query: 175 RVPVIG 181
            +P IG
Sbjct: 62  SIPRIG 67

[Close]

BLAST of Lag0033274 vs. NCBI nr
Match: KAG6427745.1 (hypothetical protein SASPL_111992 [Salvia splendens])

HSP 1 Score: 69.7 bits (169), Expect = 2.9e-08
Identity = 34/65 (52.31%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 116 GKRSWPETVGKDGEEAVKIIQRDNPKLEPVILPPSVKSQTLEYNFKRVRVYVDDNLKVTR 175
           GK SWPE VG  GEEAVK+I+++N  +  +I+PP+     L+Y   RV V+VDD+  VTR
Sbjct: 6   GKDSWPELVGVSGEEAVKVIEKENSFVNAIIVPPTQSFIPLDYRCDRVWVFVDDDGIVTR 65

Query: 176 VPVIG 181
           VP+IG
Sbjct: 66  VPLIG 70

[Close]

BLAST of Lag0033274 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 1.1e-07
Identity = 29/64 (45.31%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 117 KRSWPETVGKDGEEAVKIIQRDNPKLEPVILPPSVKSQTLEYNFKRVRVYVDDNLKVTRV 176
           K SWPE VG +G+ A  IIQ +N  ++ +++   +   T + NF RVRV+VD+N  VT+V
Sbjct: 8   KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPI-TQDLNFNRVRVFVDENRVVTQV 67

Query: 177 PVIG 181
           P IG
Sbjct: 68  PAIG 70

[Close]

BLAST of Lag0033274 vs. ExPASy Swiss-Prot
Match: P01053 (Subtilisin-chymotrypsin inhibitor-2A OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 1.3e-06
Identity = 32/84 (38.10%), Postives = 48/84 (57.14%), Query Frame = 0


Query: 97  SSVNSQGKKKMTGDEGGNSGKRSWPETVGKDGEEAVKIIQRDNPKLEPVILPPSVKSQTL 156
           SSV  + +   TG    ++ K  WPE VGK  EEA K+I +D P+ + ++LP      T+
Sbjct: 2   SSVEKKPEGVNTGAGDRHNLKTEWPELVGKSVEEAKKVILQDKPEAQIIVLPVGT-IVTM 61

Query: 157 EYNFKRVRVYVDDNLKVTRVPVIG 181
           EY   RVR++VD    + +VP +G
Sbjct: 62  EYRIDRVRLFVDKLDNIAQVPRVG 84

[Close]

BLAST of Lag0033274 vs. ExPASy Swiss-Prot
Match: P82977 (Subtilisin-chymotrypsin inhibitor WSCI OS=Triticum aestivum OX=4565 PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 1.7e-06
Identity = 31/73 (42.47%), Postives = 44/73 (60.27%), Query Frame = 0


Query: 108 TGDEGGNSGKRSWPETVGKDGEEAVKIIQRDNPKLEPVILPPSVKSQTLEYNFKRVRVYV 167
           TGD   ++ K  WPE VGK  EEA K+I +D  + + V+LP      T+EY   RVR++V
Sbjct: 15  TGDH--HNQKTEWPELVGKSVEEAKKVILQDKSEAQIVVLPVGT-IVTMEYRIDRVRLFV 74

Query: 168 DDNLKVTRVPVIG 181
           D   K+ +VP +G
Sbjct: 75  DSLDKIAQVPRVG 84

[Close]

BLAST of Lag0033274 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.8e-05
Identity = 27/65 (41.54%), Postives = 38/65 (58.46%), Query Frame = 0


Query: 116 GKRSWPETVGKDGEEAVKIIQRDNPKLEPVILPPSVKSQTLEYNFKRVRVYVDDNLKVTR 175
           GKRSWP+ VG  G  A  +I+R+NP++  VI+       T ++   RVRV+V +   V R
Sbjct: 5   GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVG-SPVTADFRCDRVRVWVTERGIVAR 64

Query: 176 VPVIG 181
            P IG
Sbjct: 65  PPAIG 68

[Close]

BLAST of Lag0033274 vs. ExPASy Swiss-Prot
Match: P81712 (Subtilisin inhibitor CLSI-I OS=Canavalia lineata OX=28957 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.8e-05
Identity = 30/64 (46.88%), Postives = 40/64 (62.50%), Query Frame = 0


Query: 117 KRSWPETVGKDGEEAVKIIQRDNPKLEPVILPPSVKSQTLEYNFKRVRVYVDDNLKVTRV 176
           K SWPE VG   EEA K I+ +   +E  ++PP     T +Y  +RVR+YVD++ KVTR 
Sbjct: 4   KTSWPELVGVTAEEAEK-IKEEMSGVEIQVVPPG-SFVTADYKPQRVRLYVDESNKVTRT 63

Query: 177 PVIG 181
           P IG
Sbjct: 64  PGIG 65

The following BLAST results are available for this feature:

BLAST of Lag0033274 vs. NCBI nr


Match Name	E-value	Identity	Description
AAT72726.1	1.5e-09	54.69	putative chymotrypsin protease inhibitor [Cucurbita maxima]	[more]
PKI46302.1	2.0e-09	56.72	hypothetical protein CRG98_033311 [Punica granatum]	[more]
CAA57307.1	3.4e-09	54.69	Pumpkin fruit trypsin inhibitor [Cucurbita maxima]	[more]
KAG6571497.1	1.0e-08	54.55	hypothetical protein SDJN03_28225, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6427745.1	2.9e-08	52.31	hypothetical protein SASPL_111992 [Salvia splendens]	[more]

BLAST of Lag0033274 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6XNP7	1.1e-07	45.31	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
P01053	1.3e-06	38.10	Subtilisin-chymotrypsin inhibitor-2A OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]
P82977	1.7e-06	42.47	Subtilisin-chymotrypsin inhibitor WSCI OS=Triticum aestivum OX=4565 PE=1 SV=2	[more]
P24076	1.8e-05	41.54	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P81712	1.8e-05	46.88	Subtilisin inhibitor CLSI-I OS=Canavalia lineata OX=28957 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 113..180 e-value: 3.6E-21 score: 77.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..67
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 95..126
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 117..180 e-value: 8.1E-19 score: 68.1
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 110..180
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 114..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0033274.1	Lag0033274.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity