Lag0033232 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033232
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionthioredoxin-like fold domain-containing protein MRL7, chloroplastic
Locationchr11: 41939967 .. 41941788 (+)
RNA-Seq ExpressionLag0033232
SyntenyLag0033232
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGTTTCTCCAAGCCTCTTCCATTTCAATGTCGGTTTCACCTCAAAATACAGTTAATAATGCTTGTACTTCGCATCGATTCTTAACCGTTCCTATTGAATATCAGGCTTTTAAGCCTTCTTGTTATTCTAGATATTGTTCGGTTAGTACTTTGCCGAACCTTGTACACTATAGCCGCAGCCAAAAACTTCCTTGCCTTGCTGTTTCATCTAAATCTAATTCAGAACATGATCCCGAACCTAGTGCTGATTCTAAAGTGAAACCAAAATCGAGAAGTCGCAGTAAGAAAGGCAAAGATCCTGAAAAGGAGGAAGAAAATGATGCTGGGATTTTCCCAACAACAATACCGAGGAAGCCTCGACGTGGTCGTAGAAGTGAAGCTGCAGCTCTTGAAGATTACGTTCGCAACACACTTGAACAAACATTTGCATCAATTCAACAGCAAAATGCAGAAGTATTGGAAAATAAAGAAAACGTACTCAAGGAAAGGGTTGTTGACAATGCTGATTCGGAAAGCAGTGACAACGAGGATGAGGATGAGGATGAGGACGAGGAGGTAGAAAGAAGTGATGGAACTGGAAGGAAGAAGAAAAAGAAGATGGTGGTAGATGAAGAAGATCCAAATTGGCCCCTGGATGCTGAAGTGGGATGGGGCGTCCGAGCATCGGAGTATTTTGAACAGCACCCTATCAAGAATGTAGTTGGGGATGATGGTGTTGAAATTGACTGGGAAGGTGAAATTGATGACAACTGGGTGCAGGAAATCAACTGTTTGGAGTGGGAGAGCTTTGCTTTTCATCCTAGTCCACTTGTTGTTCTCGTCTTCGAAAGATATAACAGGTGCTCGCTCTTCTTCTACCTTTTAAAATTTGTGATTCTTCTGTCAATAATGCATCATCAATATATTGAAACCGGTGTAGTGATACAACGGGCTTCAATGTTCTTTCGATTATCGTGTACCTCTACTATAAGTTTTAGTTGTTCTATCTTCATGAACATGATGCAACATACATCCTTGTAGTACTTAAAATTGTGGCAATACTGTGAGATGTATGTGGGAAATTTTGAAAGTTCTTGCAGAAGATATATTCCATGTTAAATTACTCATAGATTGACTATTCTGTTCCCAGAGCAAGTGATAATTGGAGGACTTTGAAGGAACTAGAGAAAGCACTACAAGTATATTGGAAAGCAAAAGATCGATTACCACCCCGGGTATCGGCTAAGTTACATGTTTTAAACTGTTTGACTCCTCAAGATTTGTCTGTCCTGATTAAGCCGAATCCTCGGAACAAATCATTTGATCCATATTTAGTCTACCTCTTCCCCCAAACAAACCTTTTGTCATATGTTTAAATAAGTTCTGTTATATCAGTTAAATTTCTCCACTTTTTGCATTGTTGATATTCTCAGCATGAATCTTATACCTTATCTATATATTCAACGATTCGCCGTTTGTTTTACCTTGTATCTGCTGACTCTAGCCTGTTAAATTTGCAATGCAGTCGATTAAGATCGACATCAACATCGAGAGAGATCTAGCCTACGCTTTGAAAGTTAGAGAATGCCCTCAAATTTTATTTTTAAGAGGCAACAGGGTCTTATACAGGGAAAAAGGTCTACATTTATTTCCTGCTACATTCCATTTCAGTTTATGTCACTGTTTCTTCATAACTTACAACCGATGCGCGTGTCTCTCGTTACATTCTTTTGCAGACTTTCGAACGGCGGATGAACTGGTTCAGATGATTGCATTTTTCTATTACAAAGCAAAGAAACCTTTATGGATAGATGATAAAGCTTTGTCTCGACCATTTTGA

mRNA sequence

ATGATGTTTCTCCAAGCCTCTTCCATTTCAATGTCGGTTTCACCTCAAAATACAGTTAATAATGCTTGTACTTCGCATCGATTCTTAACCGTTCCTATTGAATATCAGGCTTTTAAGCCTTCTTGTTATTCTAGATATTGTTCGGTTAGTACTTTGCCGAACCTTGTACACTATAGCCGCAGCCAAAAACTTCCTTGCCTTGCTGTTTCATCTAAATCTAATTCAGAACATGATCCCGAACCTAGTGCTGATTCTAAAGTGAAACCAAAATCGAGAAGTCGCAGTAAGAAAGGCAAAGATCCTGAAAAGGAGGAAGAAAATGATGCTGGGATTTTCCCAACAACAATACCGAGGAAGCCTCGACGTGGTCGTAGAAGTGAAGCTGCAGCTCTTGAAGATTACGTTCGCAACACACTTGAACAAACATTTGCATCAATTCAACAGCAAAATGCAGAAGTATTGGAAAATAAAGAAAACGTACTCAAGGAAAGGGTTGTTGACAATGCTGATTCGGAAAGCAGTGACAACGAGGATGAGGATGAGGATGAGGACGAGGAGGTAGAAAGAAGTGATGGAACTGGAAGGAAGAAGAAAAAGAAGATGGTGGTAGATGAAGAAGATCCAAATTGGCCCCTGGATGCTGAAGTGGGATGGGGCGTCCGAGCATCGGAGTATTTTGAACAGCACCCTATCAAGAATGTAGTTGGGGATGATGGTGTTGAAATTGACTGGGAAGGTGAAATTGATGACAACTGGGTGCAGGAAATCAACTGTTTGGAGTGGGAGAGCTTTGCTTTTCATCCTAGTCCACTTGTTGTTCTCGTCTTCGAAAGATATAACAGAGCAAGTGATAATTGGAGGACTTTGAAGGAACTAGAGAAAGCACTACAAGTATATTGGAAAGCAAAAGATCGATTACCACCCCGGTCGATTAAGATCGACATCAACATCGAGAGAGATCTAGCCTACGCTTTGAAAGTTAGAGAATGCCCTCAAATTTTATTTTTAAGAGGCAACAGGGTCTTATACAGGGAAAAAGACTTTCGAACGGCGGATGAACTGGTTCAGATGATTGCATTTTTCTATTACAAAGCAAAGAAACCTTTATGGATAGATGATAAAGCTTTGTCTCGACCATTTTGA

Coding sequence (CDS)

ATGATGTTTCTCCAAGCCTCTTCCATTTCAATGTCGGTTTCACCTCAAAATACAGTTAATAATGCTTGTACTTCGCATCGATTCTTAACCGTTCCTATTGAATATCAGGCTTTTAAGCCTTCTTGTTATTCTAGATATTGTTCGGTTAGTACTTTGCCGAACCTTGTACACTATAGCCGCAGCCAAAAACTTCCTTGCCTTGCTGTTTCATCTAAATCTAATTCAGAACATGATCCCGAACCTAGTGCTGATTCTAAAGTGAAACCAAAATCGAGAAGTCGCAGTAAGAAAGGCAAAGATCCTGAAAAGGAGGAAGAAAATGATGCTGGGATTTTCCCAACAACAATACCGAGGAAGCCTCGACGTGGTCGTAGAAGTGAAGCTGCAGCTCTTGAAGATTACGTTCGCAACACACTTGAACAAACATTTGCATCAATTCAACAGCAAAATGCAGAAGTATTGGAAAATAAAGAAAACGTACTCAAGGAAAGGGTTGTTGACAATGCTGATTCGGAAAGCAGTGACAACGAGGATGAGGATGAGGATGAGGACGAGGAGGTAGAAAGAAGTGATGGAACTGGAAGGAAGAAGAAAAAGAAGATGGTGGTAGATGAAGAAGATCCAAATTGGCCCCTGGATGCTGAAGTGGGATGGGGCGTCCGAGCATCGGAGTATTTTGAACAGCACCCTATCAAGAATGTAGTTGGGGATGATGGTGTTGAAATTGACTGGGAAGGTGAAATTGATGACAACTGGGTGCAGGAAATCAACTGTTTGGAGTGGGAGAGCTTTGCTTTTCATCCTAGTCCACTTGTTGTTCTCGTCTTCGAAAGATATAACAGAGCAAGTGATAATTGGAGGACTTTGAAGGAACTAGAGAAAGCACTACAAGTATATTGGAAAGCAAAAGATCGATTACCACCCCGGTCGATTAAGATCGACATCAACATCGAGAGAGATCTAGCCTACGCTTTGAAAGTTAGAGAATGCCCTCAAATTTTATTTTTAAGAGGCAACAGGGTCTTATACAGGGAAAAAGACTTTCGAACGGCGGATGAACTGGTTCAGATGATTGCATTTTTCTATTACAAAGCAAAGAAACCTTTATGGATAGATGATAAAGCTTTGTCTCGACCATTTTGA

Protein sequence

MMFLQASSISMSVSPQNTVNNACTSHRFLTVPIEYQAFKPSCYSRYCSVSTLPNLVHYSRSQKLPCLAVSSKSNSEHDPEPSADSKVKPKSRSRSKKGKDPEKEEENDAGIFPTTIPRKPRRGRRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDNEDEDEDEDEEVERSDGTGRKKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDGVEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVYWKAKDRLPPRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIAFFYYKAKKPLWIDDKALSRPF
Homology
BLAST of Lag0033232 vs. NCBI nr
Match: XP_022967425.1 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita maxima] >XP_022967426.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita maxima])

HSP 1 Score: 652.9 bits (1683), Expect = 1.7e-183
Identity = 335/383 (87.47%), Postives = 357/383 (93.21%), Query Frame = 0

Query: 1   MMFLQASSISMSVSPQNTVNNACTSHRFLTVPIEYQAFKPSCYSRYCSVSTLPNLVHYSR 60
           MMFLQA SISM VSPQ TV NA T HRFLTVPIEYQ FKPSCYSR+ SVS LPNLVH+S 
Sbjct: 1   MMFLQAPSISMPVSPQYTVYNASTLHRFLTVPIEYQTFKPSCYSRFHSVSILPNLVHFSH 60

Query: 61  SQKLPCLAVSSKSNSEHDPEPSADSKVKPKSRSRSKKGKDPEKEEENDAGIFPTTIPRKP 120
           S+KLPCL VSSKS+SEHD +PSADSKVKPKSRSRS+K K  EKEEEND  IFPTTIPRKP
Sbjct: 61  SRKLPCLVVSSKSSSEHDSQPSADSKVKPKSRSRSRKSKASEKEEENDDRIFPTTIPRKP 120

Query: 121 RRGRRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDN-EDE 180
           RRGRRSEAAALEDYVRNTLEQTFASI+Q+NAEV+ENKENV KE+V D  D ESSD+ EDE
Sbjct: 121 RRGRRSEAAALEDYVRNTLEQTFASIRQKNAEVMENKENVFKEKVDDEVDPESSDDEEDE 180

Query: 181 DEDEDEEVERSDGTGR--KKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGD 240
           DEDE+EEVE+SDGTGR  KKKKK+VV+EEDPNWPLDA+VGWGVRASEYFEQHPIKNVVGD
Sbjct: 181 DEDEEEEVEKSDGTGRNKKKKKKIVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGD 240

Query: 241 DGVEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQ 300
           DGVEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRA+DNW+TLKELEKALQ
Sbjct: 241 DGVEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQ 300

Query: 301 VYWKAKDRLPPRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQM 360
           VYW AKDRLPPRS+KIDINIERDLAYALKVRECPQILFLRGN+VLYREKDFRTADELVQM
Sbjct: 301 VYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQM 360

Query: 361 IAFFYYKAKKPLWIDDKALSRPF 381
           IAFFYYKAKKPLWI+DKAL+RPF
Sbjct: 361 IAFFYYKAKKPLWINDKALARPF 383

BLAST of Lag0033232 vs. NCBI nr
Match: XP_038888099.1 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida] >XP_038888100.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida] >XP_038888101.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida] >XP_038888102.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida] >XP_038888103.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida] >XP_038888104.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa hispida])

HSP 1 Score: 642.9 bits (1657), Expect = 1.8e-180
Identity = 326/380 (85.79%), Postives = 352/380 (92.63%), Query Frame = 0

Query: 1   MMFLQASSISMSVSPQNTVNNACTSHRFLTVPIEYQAFKPSCYSRYCSVSTLPNLVHYSR 60
           M+ LQASS+SMSVSPQ  V+NA T  RFLTVPIE Q FKPSCYSRY S S LPNLVHYS 
Sbjct: 1   MLILQASSLSMSVSPQYAVHNASTLQRFLTVPIECQTFKPSCYSRYHSHSILPNLVHYSY 60

Query: 61  SQKLPCLAVSSKSNSEHDPEPSADSKVKPKSRSRSKKGKDPEKEEENDAGIFPTTIPRKP 120
           S+KLPCLAV SKSNSEHDPEP+ADSKVK K RSRSK+ K  EKEE+NDAGIFPTTIPRKP
Sbjct: 61  SRKLPCLAVWSKSNSEHDPEPNADSKVKQKPRSRSKRSKALEKEEDNDAGIFPTTIPRKP 120

Query: 121 RRGRRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDNEDED 180
           RRGRRSEAAALEDYVRNTLEQTFASI+QQNAEV+ENKENV+KE+V D  + ESSD  DE+
Sbjct: 121 RRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVIKEKVDDKVEFESSD--DEE 180

Query: 181 EDEDEEVERSDGTGRKKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDGV 240
           EDE+EE+E+SDG GR KKKKMVV+EEDPNWPLDA+VGWGVRASEYFEQHPIKN+VGDDGV
Sbjct: 181 EDEEEEIEQSDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNIVGDDGV 240

Query: 241 EIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVYW 300
           EIDWEGEI+DNWVQEINCLEWESFAFHPSPL+VLVFERYNRA+DNW+TLKELEKALQVYW
Sbjct: 241 EIDWEGEIEDNWVQEINCLEWESFAFHPSPLIVLVFERYNRATDNWKTLKELEKALQVYW 300

Query: 301 KAKDRLPPRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIAF 360
            AKDRLPPRS+KIDINIERDLAYALKVRECPQILFLRGN+VLYREKDFRTADELVQMIAF
Sbjct: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIAF 360

Query: 361 FYYKAKKPLWIDDKALSRPF 381
           FYYKAKKP WI+DKALSRPF
Sbjct: 361 FYYKAKKPSWINDKALSRPF 378

BLAST of Lag0033232 vs. NCBI nr
Match: XP_023511559.1 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023511560.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 640.2 bits (1650), Expect = 1.1e-179
Identity = 329/381 (86.35%), Postives = 354/381 (92.91%), Query Frame = 0

Query: 1   MMFLQASSISMSVSPQNTVNNACTSHRFLTVPIEYQAFKPSCYSRYCSVSTLPNLVHYSR 60
           MMFLQA S+SMSVSPQ TV NA T HR LTVPIEYQ FKPS YSR+ SVS LPNLVH+S 
Sbjct: 1   MMFLQAPSVSMSVSPQYTVYNASTLHRSLTVPIEYQTFKPSRYSRFHSVSILPNLVHFSH 60

Query: 61  SQKLPCLAVSSKSNSEHDPEPSADSKVKPKSRSRSKKGKDPEKEEENDAGIFPTTIPRKP 120
           S+KLPCL VSSKS+SE D +PSADSKVKPKSRSRS+K K  EKEEEND  IFPTTIPRKP
Sbjct: 61  SRKLPCLVVSSKSSSEQDSQPSADSKVKPKSRSRSRKSKASEKEEENDDRIFPTTIPRKP 120

Query: 121 RRGRRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDNEDED 180
           RRGRRSEAAALEDYVRNTLEQTFASI+QQNAEV+ENKEN+ KE+V D  +SESSD+E ED
Sbjct: 121 RRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENMFKEKVDDEVESESSDDE-ED 180

Query: 181 EDEDEEVERSDGTG-RKKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDG 240
           EDE+EE+E+SDGTG  KKKKK+VV+EEDPNWPLDA+VGWGVRASEYFEQHPIKNVVGDDG
Sbjct: 181 EDEEEELEKSDGTGWNKKKKKIVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDG 240

Query: 241 VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVY 300
           VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRA+DNW+TLKELEKALQVY
Sbjct: 241 VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVY 300

Query: 301 WKAKDRLPPRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIA 360
           W AKDRLPPRS+KIDINIERDLAYALKVRECPQILFLRGN+VLYREKDFRTADELVQMIA
Sbjct: 301 WNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIA 360

Query: 361 FFYYKAKKPLWIDDKALSRPF 381
           FFYYKAKKPLWI+DKAL+RPF
Sbjct: 361 FFYYKAKKPLWINDKALARPF 380

BLAST of Lag0033232 vs. NCBI nr
Match: XP_022963814.1 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita moschata] >XP_022963815.1 thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita moschata])

HSP 1 Score: 637.1 bits (1642), Expect = 9.6e-179
Identity = 329/381 (86.35%), Postives = 355/381 (93.18%), Query Frame = 0

Query: 1   MMFLQASSISMSVSPQNTVNNACTSHRFLTVPIEYQAFKPSCYSRYCSVSTLPNLVHYSR 60
           MMFLQA S+SM VSPQ TV++A T HRFLTVPIEYQ FKPS YSR+ SVS LPNLVH+S 
Sbjct: 1   MMFLQAPSVSMPVSPQYTVHSA-TLHRFLTVPIEYQTFKPSRYSRFHSVSILPNLVHFSH 60

Query: 61  SQKLPCLAVSSKSNSEHDPEPSADSKVKPKSRSRSKKGKDPEKEEENDAGIFPTTIPRKP 120
           S+KLPCL +SSKS+SE D +PSADSKVKPKSRSRS+K K  EKEEEND  IFPTTIPRKP
Sbjct: 61  SRKLPCLVLSSKSSSEQDSQPSADSKVKPKSRSRSRKSKASEKEEENDDRIFPTTIPRKP 120

Query: 121 RRGRRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDNEDED 180
           RRGRRSEAAALEDYVRNTLEQTFASI+QQNAEV+ENKENV KE+V D  +SESSD+E ED
Sbjct: 121 RRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDDEVESESSDDE-ED 180

Query: 181 EDEDEEVERSDGTGR-KKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDG 240
           EDE+EEVE+SDGTGR KKKKK+VV+EEDPNWPLDA+VGWGVRASEYFEQHPIKNVVGDDG
Sbjct: 181 EDEEEEVEKSDGTGRNKKKKKIVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDG 240

Query: 241 VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVY 300
           VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRA+DNW+TLKELEKALQVY
Sbjct: 241 VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVY 300

Query: 301 WKAKDRLPPRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIA 360
           W AKDRLPPRS+KIDINIERDLAYALKVRECPQILFLRGN+VLYREKDFR ADELVQMIA
Sbjct: 301 WNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRMADELVQMIA 360

Query: 361 FFYYKAKKPLWIDDKALSRPF 381
           FFYYKAKKPLWI+DKAL+RPF
Sbjct: 361 FFYYKAKKPLWINDKALARPF 379

BLAST of Lag0033232 vs. NCBI nr
Match: KAG7011280.1 (Thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 636.0 bits (1639), Expect = 2.1e-178
Identity = 328/381 (86.09%), Postives = 354/381 (92.91%), Query Frame = 0

Query: 1   MMFLQASSISMSVSPQNTVNNACTSHRFLTVPIEYQAFKPSCYSRYCSVSTLPNLVHYSR 60
           MMFLQA S+SM VSPQ TV++A T HRFLTVPIEYQ FKPS YSR+ SVS LPNLVH+S 
Sbjct: 1   MMFLQAPSVSMPVSPQYTVHSA-TFHRFLTVPIEYQTFKPSRYSRFHSVSILPNLVHFSH 60

Query: 61  SQKLPCLAVSSKSNSEHDPEPSADSKVKPKSRSRSKKGKDPEKEEENDAGIFPTTIPRKP 120
           S+KLPCL +SSKS+SE D +PSADSKVKPKSRSRS+K K  EKEEEND  IFPTTIPRKP
Sbjct: 61  SRKLPCLVLSSKSSSEQDSQPSADSKVKPKSRSRSRKSKASEKEEENDDRIFPTTIPRKP 120

Query: 121 RRGRRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDNEDED 180
           RRGRRSEAAALEDYVRNTLEQTFASI+QQNAEV+ENKENV KE+V D  +SESSD+E   
Sbjct: 121 RRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDDEVESESSDDE--- 180

Query: 181 EDEDEEVERSDGTGR-KKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDG 240
           EDE+EEVE+SDG GR KKKKK+VV+EEDPNWPLDA+VGWGVRASEYFEQHPIKNVVGDDG
Sbjct: 181 EDEEEEVEKSDGAGRDKKKKKIVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDG 240

Query: 241 VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVY 300
           VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRA+DNW+TLKELEKALQVY
Sbjct: 241 VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVY 300

Query: 301 WKAKDRLPPRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIA 360
           WKAKDRLPPRS+KIDINIERDLAYALKVRECPQILFLRGN+VLYREKDFRTADELVQMIA
Sbjct: 301 WKAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIA 360

Query: 361 FFYYKAKKPLWIDDKALSRPF 381
           FFYYKAKKPLWI+DKAL+RPF
Sbjct: 361 FFYYKAKKPLWINDKALARPF 377

BLAST of Lag0033232 vs. ExPASy Swiss-Prot
Match: F4JLC1 (Thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MRL7 PE=1 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.6e-104
Identity = 201/310 (64.84%), Postives = 243/310 (78.39%), Query Frame = 0

Query: 73  SNSEHDPEPSADSKVKPKSRSRSKKGKDPEKEEENDAGIFPTTIPRKPRRGRRSEAAALE 132
           S+S  DPE    SK + +SR ++K+   P  E EN+   FPT +PRK +RGRRSEA A+E
Sbjct: 52  SDSVLDPE----SKNQTRSRRKNKEAVTPIAETENNEK-FPTKVPRKSKRGRRSEADAVE 111

Query: 133 DYVRNTLEQTFASIQQQNAEVLENKE--NVLKERVVDNADSESSDNEDEDEDEDEEVERS 192
           DYVR++LE+TF++I++QN EV ENKE  N +K+R V           DE+E+E+EE    
Sbjct: 112 DYVRSSLERTFSTIKEQNPEVFENKEKANFIKDRGV-----------DEEEEEEEE---- 171

Query: 193 DGTGRKKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDGVEIDWEGEIDD 252
                     MVV+EEDP+WP+D +VGWG++ASEYF+ HPIKNVVGDDG EIDWEGEIDD
Sbjct: 172 ----------MVVEEEDPDWPVDTDVGWGIKASEYFDTHPIKNVVGDDGSEIDWEGEIDD 231

Query: 253 NWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVYWKAKDRLPPRS 312
           +WV+EINCLEWESFAFHPSPLVVLVFERY RASDNW+TLKELEKA++VYW AKDRLPPR+
Sbjct: 232 SWVKEINCLEWESFAFHPSPLVVLVFERYKRASDNWKTLKELEKAIKVYWDAKDRLPPRA 291

Query: 313 IKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIAFFYYKAKKPLW 372
           +KID+NIE DLAYALK +ECPQILFLRGNR+LYREKDFRTADELV MIA FYYKAK+P  
Sbjct: 292 VKIDLNIETDLAYALKAKECPQILFLRGNRILYREKDFRTADELVHMIAHFYYKAKRPSC 330

Query: 373 IDDKALSRPF 381
           + DKA   P+
Sbjct: 352 V-DKANVTPY 330

BLAST of Lag0033232 vs. ExPASy Swiss-Prot
Match: Q5QLS7 (Thioredoxin-like fold domain-containing protein MRL7 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MRL7 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.4e-94
Identity = 182/306 (59.48%), Postives = 229/306 (74.84%), Query Frame = 0

Query: 77  HDPEPSADSKVKPKSRSRSKKGKDPEKEEEN---DAGIFPTTIPRKPRRGRRSEAAALED 136
           H P     S   P    R  K  +P    EN   D   FPTT PRKPRRGRRSEAAA+ED
Sbjct: 29  HHPSRLLASSAPPPPPPRPPK-PEPRTSHENLGDDTPDFPTTKPRKPRRGRRSEAAAVED 88

Query: 137 YVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDNEDEDEDEDEEVERSDGT 196
           +VR  LEQ FASI++++ EVLE K ++LK +       E SD E ++   +EE E     
Sbjct: 89  FVRGRLEQVFASIRERDPEVLEGKGDILKRK------EELSDEEGKEGTGEEEGE----- 148

Query: 197 GRKKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDGVEIDWEGEIDDNWV 256
                 K VV+EEDP+WPLDA++GWG+RASEYF++H IKNV   DGVEIDWE E+++ WV
Sbjct: 149 -----LKAVVEEEDPSWPLDADIGWGIRASEYFDKHSIKNVT-VDGVEIDWEREVEEGWV 208

Query: 257 QEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVYWKAKDRLPPRSIKI 316
           +EINCLEWESFAFHPSPL+VLVFERYNRA+DNW+ L+ELEKA +VYW +KDRLPPR++K+
Sbjct: 209 KEINCLEWESFAFHPSPLIVLVFERYNRAADNWKFLQELEKAAKVYWNSKDRLPPRTVKV 268

Query: 317 DINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIAFFYYKAKKPLWIDD 376
           D+NIERDLA+AL+V+ECPQ+LFLRGN++LYREK+ RTADELVQMIA FYY AK+P  ++ 
Sbjct: 269 DMNIERDLAFALQVKECPQLLFLRGNKILYREKELRTADELVQMIAHFYYNAKRPSCVNP 316

Query: 377 KALSRP 380
           +A++ P
Sbjct: 329 EAIAPP 316

BLAST of Lag0033232 vs. ExPASy Swiss-Prot
Match: Q9SKB6 (Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MRL7L PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 4.1e-31
Identity = 104/270 (38.52%), Postives = 149/270 (55.19%), Query Frame = 0

Query: 124 RRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKE--NVLKERVVDNADSESSDNEDEDE 183
           ++ E +  ED      E+TF   +    + +E +E    ++E V+D         +  D 
Sbjct: 78  KKEEDSDSEDEEDEVKEETFGGKEASLDDPVERREWRKTIRE-VID---------KHPDI 137

Query: 184 DEDEEVERSDGTGRKKKKK------MVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKN-- 243
           +EDEE++  +   R+K +K      +VV+EEDPNWP DA+ GWG   +++F +  IKN  
Sbjct: 138 EEDEEIDMVE--KRRKMQKLLADYPLVVNEEDPNWPEDAD-GWGFSFNQFFNKITIKNEK 197

Query: 244 ---------VVGDDG-VEIDWEGEIDDNWVQEINCL---EWESFAFHP-SPLVVLVFERY 303
                      GDD   EI W+   DDN+++ I  L   EWE   F   SPL+VLV  RY
Sbjct: 198 KEEEDDDEDSEGDDSEKEIVWQ---DDNYIRPIKDLTTAEWEEAVFKDISPLMVLVHNRY 257

Query: 304 NRASDNWRTLKELEKALQVYWKAKDRLP-PRSIKIDINIERDLAYALKVRECPQILFLRG 363
            R  +N +  +ELEKA+QV W     LP PR + +D  +E DL  ALKV   P+I+F + 
Sbjct: 258 KRPKENEKFREELEKAIQVIWNC--GLPSPRCVAVDAVVETDLVSALKVSVFPEIIFTKA 317

Query: 364 NRVLYREKDFRTADELVQMIAFFYYKAKKP 369
            ++LYREK  RTADEL +++AFFYY A KP
Sbjct: 318 GKILYREKGIRTADELSKIMAFFYYGAAKP 329

BLAST of Lag0033232 vs. ExPASy Swiss-Prot
Match: Q0DI48 (Thioredoxin-like fold domain-containing protein MRL7L homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MRL7L PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 8.8e-26
Identity = 88/244 (36.07%), Postives = 130/244 (53.28%), Query Frame = 0

Query: 167 DNADSESSDNEDEDE-----DED----------------EEVERSDGTGRKKKK------ 226
           D ++SESSD+ED+DE     DE                 E  E +D   RK K       
Sbjct: 70  DGSESESSDDEDDDEPMQMTDEQRRTLRRKIREMMDRVPETAEITDPAERKAKMLELLTK 129

Query: 227 -KMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIK-NVVGDDGVEIDWEG-----EI---D 286
            ++VV+EEDPNWP D E G G    ++F++  IK     DD  E D +G     EI   D
Sbjct: 130 YQLVVEEEDPNWPEDDEDGHGFSLGQFFDKITIKAEKKNDDDEEDDAKGNQSDKEIVWED 189

Query: 287 DNW---VQEINCLEWESFAFHP-SPLVVLVFERYNRASDNWRTLKELEKALQVYWKAKDR 346
           DN+   ++++  ++W+   F    PL+VLV  RY R  DN     +L KA++++W+    
Sbjct: 190 DNYIKPIRDVKTMDWDDTVFTDFGPLIVLVHNRYKRPQDNENARDQLVKAIEMFWEY--N 249

Query: 347 LP-PRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIAFFYYK 369
           LP PR + +D   E DL  AL V   P++LF    ++++R+K  R+A+E  +M+AFFYYK
Sbjct: 250 LPSPRCVAVDACAEPDLVKALNVSGFPEVLFTNAGKIVHRDKVVRSAEEWTRMMAFFYYK 309

BLAST of Lag0033232 vs. ExPASy TrEMBL
Match: A0A6J1HUF9 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466968 PE=4 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 8.2e-184
Identity = 335/383 (87.47%), Postives = 357/383 (93.21%), Query Frame = 0

Query: 1   MMFLQASSISMSVSPQNTVNNACTSHRFLTVPIEYQAFKPSCYSRYCSVSTLPNLVHYSR 60
           MMFLQA SISM VSPQ TV NA T HRFLTVPIEYQ FKPSCYSR+ SVS LPNLVH+S 
Sbjct: 1   MMFLQAPSISMPVSPQYTVYNASTLHRFLTVPIEYQTFKPSCYSRFHSVSILPNLVHFSH 60

Query: 61  SQKLPCLAVSSKSNSEHDPEPSADSKVKPKSRSRSKKGKDPEKEEENDAGIFPTTIPRKP 120
           S+KLPCL VSSKS+SEHD +PSADSKVKPKSRSRS+K K  EKEEEND  IFPTTIPRKP
Sbjct: 61  SRKLPCLVVSSKSSSEHDSQPSADSKVKPKSRSRSRKSKASEKEEENDDRIFPTTIPRKP 120

Query: 121 RRGRRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDN-EDE 180
           RRGRRSEAAALEDYVRNTLEQTFASI+Q+NAEV+ENKENV KE+V D  D ESSD+ EDE
Sbjct: 121 RRGRRSEAAALEDYVRNTLEQTFASIRQKNAEVMENKENVFKEKVDDEVDPESSDDEEDE 180

Query: 181 DEDEDEEVERSDGTGR--KKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGD 240
           DEDE+EEVE+SDGTGR  KKKKK+VV+EEDPNWPLDA+VGWGVRASEYFEQHPIKNVVGD
Sbjct: 181 DEDEEEEVEKSDGTGRNKKKKKKIVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGD 240

Query: 241 DGVEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQ 300
           DGVEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRA+DNW+TLKELEKALQ
Sbjct: 241 DGVEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQ 300

Query: 301 VYWKAKDRLPPRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQM 360
           VYW AKDRLPPRS+KIDINIERDLAYALKVRECPQILFLRGN+VLYREKDFRTADELVQM
Sbjct: 301 VYWNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQM 360

Query: 361 IAFFYYKAKKPLWIDDKALSRPF 381
           IAFFYYKAKKPLWI+DKAL+RPF
Sbjct: 361 IAFFYYKAKKPLWINDKALARPF 383

BLAST of Lag0033232 vs. ExPASy TrEMBL
Match: A0A6J1HJ24 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464001 PE=4 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.7e-179
Identity = 329/381 (86.35%), Postives = 355/381 (93.18%), Query Frame = 0

Query: 1   MMFLQASSISMSVSPQNTVNNACTSHRFLTVPIEYQAFKPSCYSRYCSVSTLPNLVHYSR 60
           MMFLQA S+SM VSPQ TV++A T HRFLTVPIEYQ FKPS YSR+ SVS LPNLVH+S 
Sbjct: 1   MMFLQAPSVSMPVSPQYTVHSA-TLHRFLTVPIEYQTFKPSRYSRFHSVSILPNLVHFSH 60

Query: 61  SQKLPCLAVSSKSNSEHDPEPSADSKVKPKSRSRSKKGKDPEKEEENDAGIFPTTIPRKP 120
           S+KLPCL +SSKS+SE D +PSADSKVKPKSRSRS+K K  EKEEEND  IFPTTIPRKP
Sbjct: 61  SRKLPCLVLSSKSSSEQDSQPSADSKVKPKSRSRSRKSKASEKEEENDDRIFPTTIPRKP 120

Query: 121 RRGRRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDNEDED 180
           RRGRRSEAAALEDYVRNTLEQTFASI+QQNAEV+ENKENV KE+V D  +SESSD+E ED
Sbjct: 121 RRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKENVFKEKVDDEVESESSDDE-ED 180

Query: 181 EDEDEEVERSDGTGR-KKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDG 240
           EDE+EEVE+SDGTGR KKKKK+VV+EEDPNWPLDA+VGWGVRASEYFEQHPIKNVVGDDG
Sbjct: 181 EDEEEEVEKSDGTGRNKKKKKIVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDG 240

Query: 241 VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVY 300
           VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRA+DNW+TLKELEKALQVY
Sbjct: 241 VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVY 300

Query: 301 WKAKDRLPPRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIA 360
           W AKDRLPPRS+KIDINIERDLAYALKVRECPQILFLRGN+VLYREKDFR ADELVQMIA
Sbjct: 301 WNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRMADELVQMIA 360

Query: 361 FFYYKAKKPLWIDDKALSRPF 381
           FFYYKAKKPLWI+DKAL+RPF
Sbjct: 361 FFYYKAKKPLWINDKALARPF 379

BLAST of Lag0033232 vs. ExPASy TrEMBL
Match: A0A5A7T3E0 (Polyadenylate-binding protein 2-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001700 PE=4 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.6e-177
Identity = 323/381 (84.78%), Postives = 349/381 (91.60%), Query Frame = 0

Query: 1   MMFLQASSISMSVSPQNTVNNACTSHRFLTVPIEYQAFKPSCYSRYCSVSTLPNLVHYSR 60
           M+ LQAS ISM+VSPQ  ++NA T  RFLTVP EYQ FKPSCY RY S S LPNLVH+  
Sbjct: 67  MLILQASFISMTVSPQYVLHNASTLRRFLTVPTEYQTFKPSCYFRYHSASILPNLVHHRN 126

Query: 61  SQKLPCLAVSSKSNSEHDPEPSADSKVKPKSRSRSKKGK-DPEKEEENDAGIFPTTIPRK 120
           S+KLPC AVSSKSNSEHDPEPSADSKVKPKSRSR K+ K    KEEEN AGIFPTTIPRK
Sbjct: 127 SRKLPCFAVSSKSNSEHDPEPSADSKVKPKSRSRIKRSKASSGKEEENGAGIFPTTIPRK 186

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDNEDE 180
           PRRGRRSEAAALEDYVRNTLEQTFASI+QQNAEV+ENK NV KE+V +  +SESSD+E E
Sbjct: 187 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKGNVFKEKVDNEVESESSDDE-E 246

Query: 181 DEDEDEEVERSDGTGRKKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDG 240
           DEDE+E+VE++DG GR KKKKMVV+EEDPNWPLDA+VGWGVRASEYFEQ+PIKNVVGDDG
Sbjct: 247 DEDEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQYPIKNVVGDDG 306

Query: 241 VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVY 300
           V+IDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRA+DNW+TLKELEKALQVY
Sbjct: 307 VKIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVY 366

Query: 301 WKAKDRLPPRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIA 360
           W AKDRLPPRS+KIDINIERDLAYALKVRECPQILFLRGN+VLYREKDFRTADELVQMIA
Sbjct: 367 WNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIA 426

Query: 361 FFYYKAKKPLWIDDKALSRPF 381
           FFYYKAKKPLWI+DKALSRPF
Sbjct: 427 FFYYKAKKPLWINDKALSRPF 446

BLAST of Lag0033232 vs. ExPASy TrEMBL
Match: A0A1S4DWT5 (uncharacterized protein LOC103489851 OS=Cucumis melo OX=3656 GN=LOC103489851 PE=4 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.6e-177
Identity = 323/381 (84.78%), Postives = 349/381 (91.60%), Query Frame = 0

Query: 1   MMFLQASSISMSVSPQNTVNNACTSHRFLTVPIEYQAFKPSCYSRYCSVSTLPNLVHYSR 60
           M+ LQAS ISM+VSPQ  ++NA T  RFLTVP EYQ FKPSCY RY S S LPNLVH+  
Sbjct: 13  MLILQASFISMTVSPQYVLHNASTLRRFLTVPTEYQTFKPSCYFRYHSASILPNLVHHRN 72

Query: 61  SQKLPCLAVSSKSNSEHDPEPSADSKVKPKSRSRSKKGK-DPEKEEENDAGIFPTTIPRK 120
           S+KLPC AVSSKSNSEHDPEPSADSKVKPKSRSR K+ K    KEEEN AGIFPTTIPRK
Sbjct: 73  SRKLPCFAVSSKSNSEHDPEPSADSKVKPKSRSRIKRSKASSGKEEENGAGIFPTTIPRK 132

Query: 121 PRRGRRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDNEDE 180
           PRRGRRSEAAALEDYVRNTLEQTFASI+QQNAEV+ENK NV KE+V +  +SESSD+E E
Sbjct: 133 PRRGRRSEAAALEDYVRNTLEQTFASIRQQNAEVMENKGNVFKEKVDNEVESESSDDE-E 192

Query: 181 DEDEDEEVERSDGTGRKKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDG 240
           DEDE+E+VE++DG GR KKKKMVV+EEDPNWPLDA+VGWGVRASEYFEQ+PIKNVVGDDG
Sbjct: 193 DEDEEEDVEQNDGAGRNKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQYPIKNVVGDDG 252

Query: 241 VEIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVY 300
           V+IDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRA+DNW+TLKELEKALQVY
Sbjct: 253 VKIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVY 312

Query: 301 WKAKDRLPPRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIA 360
           W AKDRLPPRS+KIDINIERDLAYALKVRECPQILFLRGN+VLYREKDFRTADELVQMIA
Sbjct: 313 WNAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTADELVQMIA 372

Query: 361 FFYYKAKKPLWIDDKALSRPF 381
           FFYYKAKKPLWI+DKALSRPF
Sbjct: 373 FFYYKAKKPLWINDKALSRPF 392

BLAST of Lag0033232 vs. ExPASy TrEMBL
Match: A0A6J1DPZ4 (thioredoxin-like fold domain-containing protein MRL7, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022750 PE=4 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 3.3e-177
Identity = 322/380 (84.74%), Postives = 348/380 (91.58%), Query Frame = 0

Query: 1   MMFLQASSISMSVSPQNTVNNACTSHRFLTVPIEYQAFKPSCYSRYCSVSTLPNLVHYSR 60
           MMFLQASSISMSVSPQ  V NA + HRFLTVP EY+ FKPSCYS+Y S   L NLV YS 
Sbjct: 1   MMFLQASSISMSVSPQYAVRNAFSLHRFLTVPTEYRTFKPSCYSKYHSDGILLNLVPYSH 60

Query: 61  SQKLPCLAVSSKSNSEHDPEPSADSKVKPKSRSRSKKGKDPEKEEENDAGIFPTTIPRKP 120
           S+KLP LAVSSKSNSEHDP+PSADSKVKPKSRS SKK K  EKEEEN A +FP+TIPRKP
Sbjct: 61  SRKLPYLAVSSKSNSEHDPQPSADSKVKPKSRSHSKKAKASEKEEENGARMFPSTIPRKP 120

Query: 121 RRGRRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKENVLKERVVDNADSESSDNEDED 180
           RRGRRSEAAALED+VRNTLEQTFASI+QQNAEV+ENKENV KER  D  + +SS + D+D
Sbjct: 121 RRGRRSEAAALEDFVRNTLEQTFASIRQQNAEVMENKENVFKERADDKVEFDSSSDNDDD 180

Query: 181 EDEDEEVERSDGTGRKKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDGV 240
           E E+EE++ SDGTGRKKKKKMVV+EEDPNWPLDA+VGWGVRASEYFEQHPIKNVVGDDGV
Sbjct: 181 E-EEEEIDESDGTGRKKKKKMVVEEEDPNWPLDADVGWGVRASEYFEQHPIKNVVGDDGV 240

Query: 241 EIDWEGEIDDNWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVYW 300
           EI+WEGEIDDNWV+EINCLEWESFAFHPSPLVVLVFERYNRA+DNW+TLKELEKALQVYW
Sbjct: 241 EINWEGEIDDNWVKEINCLEWESFAFHPSPLVVLVFERYNRATDNWKTLKELEKALQVYW 300

Query: 301 KAKDRLPPRSIKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIAF 360
            AKDRLPPRS+KIDINIERDLAYALKVRECPQILFLRGN+VLYREKDFRT+DELVQMIAF
Sbjct: 301 NAKDRLPPRSVKIDINIERDLAYALKVRECPQILFLRGNKVLYREKDFRTSDELVQMIAF 360

Query: 361 FYYKAKKPLWIDDKALSRPF 381
           FYYKAKKPLWIDDKALSRPF
Sbjct: 361 FYYKAKKPLWIDDKALSRPF 379

BLAST of Lag0033232 vs. TAIR 10
Match: AT4G28590.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 10 growth stages; BEST Arabidopsis thaliana protein match is: Thioredoxin superfamily protein (TAIR:AT2G31840.1); Has 114 Blast hits to 112 proteins in 39 species: Archae - 2; Bacteria - 0; Metazoa - 17; Fungi - 6; Plants - 67; Viruses - 0; Other Eukaryotes - 22 (source: NCBI BLink). )

HSP 1 Score: 380.9 bits (977), Expect = 1.2e-105
Identity = 201/310 (64.84%), Postives = 243/310 (78.39%), Query Frame = 0

Query: 73  SNSEHDPEPSADSKVKPKSRSRSKKGKDPEKEEENDAGIFPTTIPRKPRRGRRSEAAALE 132
           S+S  DPE    SK + +SR ++K+   P  E EN+   FPT +PRK +RGRRSEA A+E
Sbjct: 52  SDSVLDPE----SKNQTRSRRKNKEAVTPIAETENNEK-FPTKVPRKSKRGRRSEADAVE 111

Query: 133 DYVRNTLEQTFASIQQQNAEVLENKE--NVLKERVVDNADSESSDNEDEDEDEDEEVERS 192
           DYVR++LE+TF++I++QN EV ENKE  N +K+R V           DE+E+E+EE    
Sbjct: 112 DYVRSSLERTFSTIKEQNPEVFENKEKANFIKDRGV-----------DEEEEEEEE---- 171

Query: 193 DGTGRKKKKKMVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKNVVGDDGVEIDWEGEIDD 252
                     MVV+EEDP+WP+D +VGWG++ASEYF+ HPIKNVVGDDG EIDWEGEIDD
Sbjct: 172 ----------MVVEEEDPDWPVDTDVGWGIKASEYFDTHPIKNVVGDDGSEIDWEGEIDD 231

Query: 253 NWVQEINCLEWESFAFHPSPLVVLVFERYNRASDNWRTLKELEKALQVYWKAKDRLPPRS 312
           +WV+EINCLEWESFAFHPSPLVVLVFERY RASDNW+TLKELEKA++VYW AKDRLPPR+
Sbjct: 232 SWVKEINCLEWESFAFHPSPLVVLVFERYKRASDNWKTLKELEKAIKVYWDAKDRLPPRA 291

Query: 313 IKIDINIERDLAYALKVRECPQILFLRGNRVLYREKDFRTADELVQMIAFFYYKAKKPLW 372
           +KID+NIE DLAYALK +ECPQILFLRGNR+LYREKDFRTADELV MIA FYYKAK+P  
Sbjct: 292 VKIDLNIETDLAYALKAKECPQILFLRGNRILYREKDFRTADELVHMIAHFYYKAKRPSC 330

Query: 373 IDDKALSRPF 381
           + DKA   P+
Sbjct: 352 V-DKANVTPY 330

BLAST of Lag0033232 vs. TAIR 10
Match: AT2G31840.1 (Thioredoxin superfamily protein )

HSP 1 Score: 137.1 bits (344), Expect = 2.9e-32
Identity = 104/270 (38.52%), Postives = 149/270 (55.19%), Query Frame = 0

Query: 124 RRSEAAALEDYVRNTLEQTFASIQQQNAEVLENKE--NVLKERVVDNADSESSDNEDEDE 183
           ++ E +  ED      E+TF   +    + +E +E    ++E V+D         +  D 
Sbjct: 78  KKEEDSDSEDEEDEVKEETFGGKEASLDDPVERREWRKTIRE-VID---------KHPDI 137

Query: 184 DEDEEVERSDGTGRKKKKK------MVVDEEDPNWPLDAEVGWGVRASEYFEQHPIKN-- 243
           +EDEE++  +   R+K +K      +VV+EEDPNWP DA+ GWG   +++F +  IKN  
Sbjct: 138 EEDEEIDMVE--KRRKMQKLLADYPLVVNEEDPNWPEDAD-GWGFSFNQFFNKITIKNEK 197

Query: 244 ---------VVGDDG-VEIDWEGEIDDNWVQEINCL---EWESFAFHP-SPLVVLVFERY 303
                      GDD   EI W+   DDN+++ I  L   EWE   F   SPL+VLV  RY
Sbjct: 198 KEEEDDDEDSEGDDSEKEIVWQ---DDNYIRPIKDLTTAEWEEAVFKDISPLMVLVHNRY 257

Query: 304 NRASDNWRTLKELEKALQVYWKAKDRLP-PRSIKIDINIERDLAYALKVRECPQILFLRG 363
            R  +N +  +ELEKA+QV W     LP PR + +D  +E DL  ALKV   P+I+F + 
Sbjct: 258 KRPKENEKFREELEKAIQVIWNC--GLPSPRCVAVDAVVETDLVSALKVSVFPEIIFTKA 317

Query: 364 NRVLYREKDFRTADELVQMIAFFYYKAKKP 369
            ++LYREK  RTADEL +++AFFYY A KP
Sbjct: 318 GKILYREKGIRTADELSKIMAFFYYGAAKP 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022967425.11.7e-18387.47thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita m... [more]
XP_038888099.11.8e-18085.79thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Benincasa h... [more]
XP_023511559.11.1e-17986.35thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita p... [more]
XP_022963814.19.6e-17986.35thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita m... [more]
KAG7011280.12.1e-17886.09Thioredoxin-like fold domain-containing protein MRL7, chloroplastic [Cucurbita a... [more]
Match NameE-valueIdentityDescription
F4JLC11.6e-10464.84Thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Arabidops... [more]
Q5QLS73.4e-9459.48Thioredoxin-like fold domain-containing protein MRL7 homolog, chloroplastic OS=O... [more]
Q9SKB64.1e-3138.52Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Arabidop... [more]
Q0DI488.8e-2636.07Thioredoxin-like fold domain-containing protein MRL7L homolog, chloroplastic OS=... [more]
Match NameE-valueIdentityDescription
A0A6J1HUF98.2e-18487.47thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Cucurbita... [more]
A0A6J1HJ244.7e-17986.35thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Cucurbita... [more]
A0A5A7T3E02.6e-17784.78Polyadenylate-binding protein 2-binding protein 2 OS=Cucumis melo var. makuwa OX... [more]
A0A1S4DWT52.6e-17784.78uncharacterized protein LOC103489851 OS=Cucumis melo OX=3656 GN=LOC103489851 PE=... [more]
A0A6J1DPZ43.3e-17784.74thioredoxin-like fold domain-containing protein MRL7, chloroplastic isoform X1 O... [more]
Match NameE-valueIdentityDescription
AT4G28590.11.2e-10564.84FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT2G31840.12.9e-3238.52Thioredoxin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 136..164
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 244..368
e-value: 1.1E-6
score: 30.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 162..205
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 189..205
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..127
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..109
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 172..188
NoneNo IPR availablePANTHERPTHR34669:SF2THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7, CHLOROPLASTICcoord: 34..379
IPR044701Thioredoxin-like fold domain-containing protein MRL7/MRL7LPANTHERPTHR34669THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7L, CHLOROPLASTICcoord: 34..379
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 253..359

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033232.1Lag0033232.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0009570 chloroplast stroma