Lag0033213 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0033213 vs. NCBI nr
Match: XP_038887438.1 (subtilisin-like protease SBT1.2 [Benincasa hispida])

HSP 1 Score: 1432.9 bits (3708), Expect = 0.0e+00
Identity = 713/771 (92.48%), Postives = 739/771 (95.85%), Query Frame = 0

Query: 1   MDFNTQMAFLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQT 60
           MDFNTQ+ FL  LFLCFLSL VQ+N + LQTYIIQLHP GLT+SL+DSKLQWHLSFLE+T
Sbjct: 1   MDFNTQLGFL--LFLCFLSLHVQSNTTALQTYIIQLHPHGLTTSLYDSKLQWHLSFLERT 60

Query: 61  LSAEEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFL 120
           LS EEDSSSRLLYSYS AMEGFAAQLSETELEYLKKLPDVVAVRADRK++IQTTYSHKFL
Sbjct: 61  LSVEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYQIQTTYSHKFL 120

Query: 121 GLSSGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSS 180
           GLS G QGVW KSSMGQGAIVGVLDTGVWPESPSFSD KMPPIP KWRGACQEGQDFNSS
Sbjct: 121 GLSVGRQGVWQKSSMGQGAIVGVLDTGVWPESPSFSDYKMPPIPPKWRGACQEGQDFNSS 180

Query: 181 ICNRKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGN 240
            CNRKLIGAKFFIKGHHVASS PSDV QEYVSPRDSHGHGTHTSSTAAGA+V EASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSPPSDVVQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI+DGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIKDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAI 360
           IAIGSFRAMQHGISVVCAAGNNGP QSSVANVAPWITTIGA TLDRRFPAI++LSNGE I
Sbjct: 301 IAIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVQLSNGETI 360

Query: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEK 420
           YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMV+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVVCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
           GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLK+YINTTTNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKSYINTTTNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLP+DSR S
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPDDSRSS 540

Query: 541 NFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDG-NKPA 600
           NFTVMSGTSMACPHVSGI+ALIHS HP WTPAAIKSAIMTTADVTDHFGKPILDG NKPA
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSVHPNWTPAAIKSAIMTTADVTDHFGKPILDGNNKPA 600

Query: 601 GVFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMN 660
           GVFAMGAGHVNPTKAIDPGL+YDIKPYEY+IHLCALGYTHSEIFI+THMNVSCHKV+QMN
Sbjct: 601 GVFAMGAGHVNPTKAIDPGLIYDIKPYEYIIHLCALGYTHSEIFIVTHMNVSCHKVVQMN 660

Query: 661 KGFSLNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKR 720
           KGF+LNYPSMSV+FK GTTSKMVSRRLTNVG+PNSIY VEV APEGVRVRVKPR LVFK 
Sbjct: 661 KGFTLNYPSMSVIFKHGTTSKMVSRRLTNVGTPNSIYKVEVAAPEGVRVRVKPRRLVFKH 720

Query: 721 VNQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTWKN 771
           VNQSLNYKVWF SEKG+EGRKVSSTEGHLTW+HSEN KY+VRSPIVVTWKN
Sbjct: 721 VNQSLNYKVWFTSEKGKEGRKVSSTEGHLTWLHSENSKYKVRSPIVVTWKN 769

BLAST of Lag0033213 vs. NCBI nr
Match: XP_023532819.1 (subtilisin-like protease SBT1.2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1431.0 bits (3703), Expect = 0.0e+00
Identity = 713/770 (92.60%), Postives = 743/770 (96.49%), Query Frame = 0

Query: 1   MDFNTQMAFLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQT 60
           MDFN+QM  LSTLFLCFLSLQVQA+ STLQTY+IQLHP GLTSS F SK QWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQADTSTLQTYLIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFL 120
           LSAEEDSSSRLLYSYS AMEGFAAQLSETELEYLKKLPDVVAVRADRK+EIQTTYS KFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120

Query: 121 GLSSGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSS 180
           GLS GTQGVW+ SSMG+GAIVGVLDTGVWPESPSFSDS+MPPIP+KWRGACQEGQDFNSS
Sbjct: 121 GLSLGTQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180

Query: 181 ICNRKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGN 240
            CN+KLIGAKFFIKGHHVASS PSDV QEYVSPRDSHGHGTHTSSTAAGA+V EASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAP AHIAVYKVCWFSGCYSSDIVAAMD+AIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAI 360
           IAIGSFRAMQHGISV CAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAI+RL NGE I
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLRNGEFI 360

Query: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEK 420
           YGESMYPGNKFK ATKELEVVYLTGGQMGGELCLKGSLPREQV+GK+VLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
           GQIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDGNKPAG 600
           NFTVMSGTSMACPHVSGI+ALIHSAHPKWT AAIKSAIMTTA+VTDH+GKPILDG+KPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTAAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGH+NPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRV 720
           GFSLNYPSMSVVFK  TTSK +SRRLTNVGSPNSIYSVEV AP+GV+VRVKPR LVFK V
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAISRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTWKN 771
           NQSLNYKVWFMSEKG+EGRKVSS+EGHLTWVHSENGKY+VRSP+VVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVSSSEGHLTWVHSENGKYKVRSPMVVTWKN 770

BLAST of Lag0033213 vs. NCBI nr
Match: KAG6606345.1 (Subtilisin-like protease 1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1431.0 bits (3703), Expect = 0.0e+00
Identity = 714/770 (92.73%), Postives = 742/770 (96.36%), Query Frame = 0

Query: 1   MDFNTQMAFLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQT 60
           MDFN+QM  LSTLFLCFLSLQVQAN  TLQTYIIQLHP GLTSS F SK QWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFL 120
           LSAEEDSSSRLLYSYS AMEGFAAQLSETE+EYLKKLPDVVAVRADRK+EIQTTYS KFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120

Query: 121 GLSSGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSS 180
           GLS G+QGVW+ SSMG+GAIVGVLDTGVWPESPSFSDS+MPPIP+KWRGACQEGQDFNSS
Sbjct: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180

Query: 181 ICNRKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGN 240
            CNRKLIGAKFFIKGHHVASS PSDV QEYVSPRDSHGHGTHTSSTAAGA+V EASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAP AHIAVYKVCWFSGCYSSDIVAAMD+AIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAI 360
           IAIGSFRAMQHGISV CAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAI+RLSNGE I
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360

Query: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEK 420
           YGESMYPGNKFK ATKELEVVYLTGGQMGGELCLKGSLPREQV+GK+VLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
           GQIVKESGGAAMILAN+ INLEEDLVDVHVLPATLIGFAEANRLKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDGNKPAG 600
           NFTVMSGTSMACPHVSGI+ALIHSAHPKWTPAAIKSAIMTTA+VTDH+GKPILDG+KPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGH+NPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRV 720
           GFSLNYPSMSVVFK  TTSK VSRRLTNVGSPNSIYSVEV AP+GV+VRVKPR LVFK V
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTWKN 771
           NQSLNYKVWFMSEKG+EG+ VSS+EGHLTWVHSENGKY+VRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770

BLAST of Lag0033213 vs. NCBI nr
Match: XP_022995265.1 (subtilisin-like protease SBT1.2 [Cucurbita maxima])

HSP 1 Score: 1431.0 bits (3703), Expect = 0.0e+00
Identity = 713/770 (92.60%), Postives = 744/770 (96.62%), Query Frame = 0

Query: 1   MDFNTQMAFLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQT 60
           MDFN+QM  LSTLFLCFLSLQVQAN STLQTYIIQLHP GLTSS F SK QWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQANTSTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFL 120
           LSAEEDSSSRLLYSYS AMEGFAAQLSETE+EYLKKLPDVVAVRAD+K+EIQTTYS KFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADKKYEIQTTYSPKFL 120

Query: 121 GLSSGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSS 180
           GLS G+QGVW+ S+MG+GAIVGVLDTGVWPESPSFSDS+MP IP+KWRGACQEGQDFNSS
Sbjct: 121 GLSLGSQGVWFNSAMGEGAIVGVLDTGVWPESPSFSDSRMPSIPQKWRGACQEGQDFNSS 180

Query: 181 ICNRKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGN 240
            CN+KLIGAKFFIKGHHVASS PSDV QEYVSPRDSHGHGTHTSSTAAGA+V EASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSPPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAP AHIAVYKVCWFSGCYSSDIVAAMD+AIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAI 360
           IAIGSFRAMQHGISV CAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAI+RLSNGE I
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360

Query: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEK 420
           YGESMYPGNKFK ATKELEVVYLTGGQMGGELCLKGSLPREQV+GK+VLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
           GQIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGF+EANRLKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFSEANRLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDGNKPAG 600
           NFTVMSGTSMACPHVSGI+ALIHSAHPKWTPAAIKSAIMTTA+VTDH+GKPILDG+KPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGH+NPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRV 720
           GFSLNYPSMSVVFK  TTSK VSRRLTNVGSPNSIYSVEV AP+GV+VRVKPR LVFK V
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTWKN 771
           NQSLNYKVWFMSEKG+EGRKVSS+EGHLTWVHSENGKY+VRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770

BLAST of Lag0033213 vs. NCBI nr
Match: XP_008447369.1 (PREDICTED: subtilisin-like protease SBT1.2 [Cucumis melo] >KAA0037948.1 subtilisin-like protease SBT1.2 [Cucumis melo var. makuwa] >TYK19024.1 subtilisin-like protease SBT1.2 [Cucumis melo var. makuwa])

HSP 1 Score: 1430.2 bits (3701), Expect = 0.0e+00
Identity = 716/770 (92.99%), Postives = 737/770 (95.71%), Query Frame = 0

Query: 1   MDFNTQMAFLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQT 60
           MDFNT+M FL  LFL FLSL VQ N STLQTYIIQLHP GL +S+FDSKLQWHLSFLEQT
Sbjct: 1   MDFNTRMGFL--LFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFL 120
           LSAEEDSSSRLLYSYS AMEGFAAQL+ETELEYLKKLPDVVAVR DRK++IQTTYSHKFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFL 120

Query: 121 GLSSGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSS 180
           GLS GTQGVW KSSMGQGAIVG+LDTGVWPESPSFSDSKMPPIP+KWRGACQEGQDFNSS
Sbjct: 121 GLSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSS 180

Query: 181 ICNRKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGN 240
            CNRKLIGAKFFIKGHHVASSLPSDV QEYVSPRDSHGHGTHTSSTAAGA+V +ASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAI 360
           IAIGSFRAMQHGISVVCAAGNNGP QSSVANVAPWITTIGA TLDRRFPAI+RLSNGEAI
Sbjct: 301 IAIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAI 360

Query: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEK 420
           YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPRE+VQGKMV+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
           GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPT LPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDGNKPAG 600
           NFTVMSGTSMACPHVSGI+ALIHSAHPKWTPAAIKSAIMTTADVTDHFGK ILDGNKPA 
Sbjct: 541 NFTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPAD 600

Query: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGHVNPTKAIDPGLVYDIKPYEY+IHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRV 720
           GF+LNYPSMSV+FK GTTSKMVSRRLTNVGS NSIY V+V APEGVRVRVKPR LVFK V
Sbjct: 661 GFTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHV 720

Query: 721 NQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTWKN 771
           NQSLNYKVWFMSEKG+EGRKV  TEG LTW+H EN KY+VRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of Lag0033213 vs. ExPASy Swiss-Prot
Match: O64495 (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana OX=3702 GN=SBT1.2 PE=2 SV=1)

HSP 1 Score: 1059.3 bits (2738), Expect = 2.1e-308
Identity = 519/767 (67.67%), Postives = 634/767 (82.66%), Query Frame = 0

Query: 14  FLCFLSLQVQANASTL---QTYIIQLHPLGLTSSLFDSKLQWHLSFLEQTL----SAEED 73
           FLC + L   +++S +   QTYI+QLHP   T+  F SK  WHLSFL++ +      EE+
Sbjct: 7   FLCIIFLLFCSSSSEILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 66

Query: 74  SSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFLGLSS-G 133
            SSRLLYSY  A+EGFAAQL+E+E E L+  P+VVAVR D   ++QTTYS+KFLGL   G
Sbjct: 67  PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 126

Query: 134 TQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSSICNRK 193
             GVW KS  GQG I+GVLDTGVWPESPSF D+ MP IP+KW+G CQEG+ F+SS CNRK
Sbjct: 127 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 186

Query: 194 LIGAKFFIKGHHVASSLPS--DVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGNGAG 253
           LIGA+FFI+GH VA+S     ++P+EY+S RDS GHGTHT+ST  G++V  A+V GNGAG
Sbjct: 187 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 246

Query: 254 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAI 313
           VA+GMAPGAHIAVYKVCWF+GCYSSDI+AA+D AI+D VD+LSLSLGGFP+P +DD+IAI
Sbjct: 247 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 306

Query: 314 GSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAIYGE 373
           G+FRAM+ GISV+CAAGNNGPI+SSVAN APW++TIGA TLDRRFPA++RL+NG+ +YGE
Sbjct: 307 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 366

Query: 374 SMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEKGQI 433
           S+YPG   K A +E+EV+Y+TGG  G E CL+GSLPRE+++GKMV+CDRGVNGRSEKG+ 
Sbjct: 367 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 426

Query: 434 VKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGT 493
           VKE+GG AMILAN+EIN EED +DVH+LPATLIG+ E+  LKAY+N T  PKARI FGGT
Sbjct: 427 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 486

Query: 494 VIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNFT 553
           VIGRSRAP VAQFS+RGPSL+NPS LKPD+IAPGVNIIAAWPQNLGPTGLP DSRR NFT
Sbjct: 487 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 546

Query: 554 VMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDGNKPAGVFA 613
           VMSGTSM+CPHVSGI+ALI SA+P W+PAAIKSA+MTTAD+ D  GK I DGNKPAGVFA
Sbjct: 547 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 606

Query: 614 MGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGFS 673
           +GAGHVNP KAI+PGLVY+I+P +Y+ +LC LG+T S+I  ITH NVSC+ +L+ N GFS
Sbjct: 607 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 666

Query: 674 LNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRVNQS 733
           LNYPS++V+FKRG T++M++RR+TNVGSPNSIYSV V APEG++V V P+ LVFK V+Q+
Sbjct: 667 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 726

Query: 734 LNYKVWFMSEKGREGRKVSS-TEGHLTWVHSENGKYRVRSPIVVTWK 770
           L+Y+VWF+ +K   G KV+S  +G LTWV+S N   RVRSPI VT K
Sbjct: 727 LSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPISVTLK 773

BLAST of Lag0033213 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 1.7e-185
Identity = 362/765 (47.32%), Postives = 484/765 (63.27%), Query Frame = 0

Query: 12  TLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQTLSAEEDSSSRL 71
           T+   FL L +   A   +TYII+++      S F +   W+ S L        +S S L
Sbjct: 12  TIITTFLFLLLHTTAK--KTYIIRVNHSDKPES-FLTHHDWYTSQL--------NSESSL 71

Query: 72  LYSYSKAMEGFAAQLSETELE-YLKKLPDVVAVRADRKHEIQTTYSHKFLGLSSGTQGVW 131
           LY+Y+ +  GF+A L  TE +  L     ++ +  D  + + TT + +FLGL+S   GV 
Sbjct: 72  LYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNS-EFGVH 131

Query: 132 YKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSSICNRKLIGAK 191
              S   G I+GVLDTGVWPES SF D+ MP IP KW+G C+ G DF+S +CN+KLIGA+
Sbjct: 132 DLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGAR 191

Query: 192 FFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGNGAGVAQGMAP 251
            F KG  +AS       +E VSPRD  GHGTHTS+TAAG+AV  AS  G  AG A+GMA 
Sbjct: 192 SFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMAT 251

Query: 252 GAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQ 311
            A +A YKVCW +GC+ SDI+AAMD AI DGVD+LSLSLGG   P++ D+IAIG+F AM+
Sbjct: 252 RARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAME 311

Query: 312 HGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAIYGESMYPGNK 371
            G+ V C+AGN+GP ++SVANVAPW+ T+GA TLDR FPA   L NG+ + G S+Y G  
Sbjct: 312 RGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG-- 371

Query: 372 FKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEKGQIVKESGGA 431
               TK LE+VY  G      LCL GSL    V+GK+V+CDRGVN R EKG +V+++GG 
Sbjct: 372 VGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGL 431

Query: 432 AMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVIGRSRA 491
            MI+AN+  + EE + D H+LPA  +G    + L+ Y+ + + P A + F GTV+    +
Sbjct: 432 GMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS 491

Query: 492 PSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNFTVMSGTSM 551
           P VA FSSRGP+   P  LKPDVI PGVNI+A W   +GPTGL +DSRR+ F +MSGTSM
Sbjct: 492 PVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSM 551

Query: 552 ACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDG--NKPAGVFAMGAGH 611
           +CPH+SG++ L+ +AHP+W+P+AIKSA+MTTA V D+   P+ D   N  +  +A G+GH
Sbjct: 552 SCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGH 611

Query: 612 VNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIF-IITHMNVSCHKVLQMNKGFSLNYP 671
           V+P KA+ PGLVYDI   EY+  LC+L YT   I  I+   +V+C K  + +    LNYP
Sbjct: 612 VDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFSDPGQLNYP 671

Query: 672 SMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRVNQSLNYK 731
           S SV+F  G      +R +TNVG+ +S+Y V V     V + VKP  L FK V +   Y 
Sbjct: 672 SFSVLF-GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYT 731

Query: 732 VWFMSEKGREGRKVSSTE----GHLTWVHSENGKYRVRSPIVVTW 769
           V F+S+KG     VS T     G +TW    N ++ VRSP+  +W
Sbjct: 732 VTFVSKKG-----VSMTNKAEFGSITW---SNPQHEVRSPVAFSW 751

BLAST of Lag0033213 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 2.2e-180
Identity = 352/773 (45.54%), Postives = 489/773 (63.26%), Query Frame = 0

Query: 8   AFLST----LFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQTLSA 67
           +FLS+    L LC     V +++S   TYI+ +    + SS FD    W+ S L      
Sbjct: 4   SFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSS-FDLHSNWYDSSLRSI--- 63

Query: 68  EEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFLGLS 127
               S+ LLY+Y  A+ GF+ +L++ E + L   P V++V  + ++E+ TT +  FLGL 
Sbjct: 64  --SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD 123

Query: 128 SGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSSICN 187
             T  ++ ++      +VGVLDTGVWPES S+SD    PIP  W+G C+ G +F +S+CN
Sbjct: 124 EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCN 183

Query: 188 RKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGNGAG 247
           RKLIGA+FF +G+  ++  P D  +E  SPRD  GHGTHTSSTAAG+ V  AS+ G  +G
Sbjct: 184 RKLIGARFFARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 243

Query: 248 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAI 307
            A+GMAP A +AVYKVCW  GC+SSDI+AA+D AI D V++LS+SLGG    ++ D +AI
Sbjct: 244 TARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAI 303

Query: 308 GSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAIYGE 367
           G+F AM+ GI V C+AGN GP  SS++NVAPWITT+GA TLDR FPA+  L NG+   G 
Sbjct: 304 GAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGV 363

Query: 368 SMYPGNKFKQATKELEVVYL--TGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEKG 427
           S++ G       K L  +Y         G LC+ G+L  E+V+GK+V+CDRG+N R +KG
Sbjct: 364 SLFKGEALPD--KLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKG 423

Query: 428 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 487
            +VK +GG  MILAN+  N EE + D H+LPAT +G    + ++ Y+ T  NP A I   
Sbjct: 424 DVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISIL 483

Query: 488 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSN 547
           GTV+G   +P VA FSSRGP+   P+ LKPD+IAPGVNI+AAW    GPTGL  DSRR  
Sbjct: 484 GTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVE 543

Query: 548 FTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILD--GNKPA 607
           F ++SGTSM+CPHVSG++AL+ S HP+W+PAAI+SA+MTTA  T   GKP+LD    KP+
Sbjct: 544 FNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPS 603

Query: 608 GVFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMN 667
             F  GAGHV+PT A +PGL+YD+   +Y+  LCAL YT  +I  ++  N +C      +
Sbjct: 604 TPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTC----DPS 663

Query: 668 KGFS---LNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMA-PEGVRVRVKPRHL 727
           K +S   LNYPS +V    G  +   +R +T+VG   + YSV+V +   GV++ V+P  L
Sbjct: 664 KSYSVADLNYPSFAVNVD-GVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 723

Query: 728 VFKRVNQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTW 769
            FK  N+  +Y V F  +  +     S++ G + W    +GK+ V SP+ ++W
Sbjct: 724 NFKEANEKKSYTVTFTVDSSKPSG--SNSFGSIEW---SDGKHVVGSPVAISW 756

BLAST of Lag0033213 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 5.3e-179
Identity = 346/781 (44.30%), Postives = 490/781 (62.74%), Query Frame = 0

Query: 7   MAFLSTLF--LCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQTLSAE 66
           MAF    F  L   S    A++S   TYI+ +       S+F +   W+ S    +L++ 
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDH-EAKPSIFPTHFHWYTS----SLASL 60

Query: 67  EDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFLGL-S 126
             S   ++++Y     GF+A+L+  +   L   P V++V  ++   + TT S +FLGL S
Sbjct: 61  TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRS 120

Query: 127 SGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSSICN 186
           +   G+  +S  G   ++GV+DTGVWPE PSF D  + P+P KW+G C   QDF  S CN
Sbjct: 121 TDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACN 180

Query: 187 RKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGNGAG 246
           RKL+GA+FF  G+   +   ++   E+ SPRDS GHGTHT+S +AG  V  AS  G   G
Sbjct: 181 RKLVGARFFCGGYEATNGKMNET-TEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHG 240

Query: 247 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAI 306
           VA GMAP A +A YKVCW SGCY SDI+AA D+A+ DGVD++SLS+GG  +P++ D+IAI
Sbjct: 241 VAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAI 300

Query: 307 GSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAIYGE 366
           G+F A+  GI V  +AGN GP   +V NVAPW+TT+GA T+DR FPA ++L NG+ I G 
Sbjct: 301 GAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGV 360

Query: 367 SMYPGNKFKQATKELEVVYLTGGQMGGE-----LCLKGSLPREQVQGKMVLCDRGVNGRS 426
           S+Y G       +   +VY  G  +GG+     LCL+GSL    V+GK+VLCDRG+N R+
Sbjct: 361 SVYGGPGLDPG-RMYPLVY-GGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRA 420

Query: 427 EKGQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYIN------TTT 486
            KG+IV+++GG  MI+AN   + E  + D HVLPAT +G +  + ++ YI+      ++ 
Sbjct: 421 TKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSK 480

Query: 487 NPKARIQFGGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTG 546
           +P A I F GT +G   AP VA FS+RGP+   P  LKPDVIAPG+NI+AAWP  +GP+G
Sbjct: 481 HPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 540

Query: 547 LPEDSRRSNFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPI 606
           +  D+RR+ F ++SGTSMACPHVSG++AL+ +AHP W+PAAI+SA++TTA   D+ G+P+
Sbjct: 541 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 600

Query: 607 LDGN--KPAGVFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNV 666
           +D +    + V   G+GHV+PTKA+DPGLVYDI  Y+Y+  LC   YT + I  IT    
Sbjct: 601 MDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 660

Query: 667 SCHKVLQMNKGFSLNYPSMSVVFKRGTTSKMVS---RRLTNVGSPNSIYSVEVMAPEGVR 726
            C    +     +LNYPS SVVF++   SKM +   R +TNVG  +S+Y +++  P G  
Sbjct: 661 DCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTT 720

Query: 727 VRVKPRHLVFKRVNQSLNYKVWFMSEKGREGRKVSSTE-GHLTWVHSENGKYRVRSPIVV 768
           V V+P  L F+RV Q L++ V   + + +     ++ E GH+ W    +GK  V SP+VV
Sbjct: 721 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVW---SDGKRNVTSPLVV 770

BLAST of Lag0033213 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 9.4e-176
Identity = 354/774 (45.74%), Postives = 478/774 (61.76%), Query Frame = 0

Query: 9   FLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQTLSAEEDSS 68
           F+  L LCF S    +++  L++YI+ +       SLF S   WH+S L    S+ + ++
Sbjct: 11  FVFPLLLCFFS-PSSSSSDGLESYIVHVQ-RSHKPSLFSSHNNWHVSLLRSLPSSPQPAT 70

Query: 69  SRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFLGLSSGTQG 128
             LLYSYS+A+ GF+A+LS  +   L++ P V++V  D+  EI TT++  FLG S  + G
Sbjct: 71  --LLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNS-G 130

Query: 129 VWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSSICNRKLIG 188
           +W  S+ G+  IVGVLDTG+WPE PSFSDS + PIP  W+G C+ G DF +S CNRKLIG
Sbjct: 131 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 190

Query: 189 AKFFIKGHHV-ASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGNGAGVAQG 248
           A+ F +G+    +       +E  SPRD+ GHGTHT+STAAG+ V  AS++    G A G
Sbjct: 191 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 250

Query: 249 MAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLG--GFPLPFFDDSIAIGS 308
           MA  A IA YK+CW  GCY SDI+AAMD A+ DGV ++SLS+G  G    +  DSIAIG+
Sbjct: 251 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 310

Query: 309 FRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAIYGESM 368
           F A +HGI V C+AGN+GP   +  N+APWI T+GAST+DR F A     +G+   G S+
Sbjct: 311 FGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSL 370

Query: 369 YPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEKGQIVK 428
           Y G     +  +L +VY   G  G  LC  G L    V+GK+VLCDRG N R EKG  VK
Sbjct: 371 YAGESLPDS--QLSLVY--SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVK 430

Query: 429 ESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVI 488
            +GGA MILAN+  + EE   D H++PAT++G    ++++ YI T+ +P A+I F GT+I
Sbjct: 431 LAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLI 490

Query: 489 GRS-RAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNFTV 548
           G S  +P VA FSSRGP+   P  LKPDVIAPGVNI+A W   +GPT L  D RR  F +
Sbjct: 491 GPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNI 550

Query: 549 MSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILD--GNKPAGVF 608
           +SGTSM+CPHVSG++AL+  AHP W+PAAIKSA++TTA   ++ G+PI D    K +  F
Sbjct: 551 ISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSF 610

Query: 609 AMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITH---MNVSCHKVLQMN 668
             GAGHV+P KA++PGLVYDI+  EYV  LCA+GY    I +      +  +C +  ++ 
Sbjct: 611 IHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDAC-ETSKLR 670

Query: 669 KGFSLNYPSMSVVFKRGTTSKMVSRRLTNVGS-PNSIYSVEVMAPEGVRVRVKPRHLVFK 728
               LNYPS SVVF          R + NVGS  +++Y V V +P  V + V P  L F 
Sbjct: 671 TAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFS 730

Query: 729 RVNQSLNYKVWFMSEKGREGRKVSSTEGH----LTWVHSENGKYRVRSPIVVTW 769
           +    L Y+V F S     G  V S  GH    + W    +G++ V+SP+ V W
Sbjct: 731 KEKSVLEYEVTFKSVV--LGGGVGSVPGHEFGSIEWT---DGEHVVKSPVAVQW 769

BLAST of Lag0033213 vs. ExPASy TrEMBL
Match: A0A6J1K3M4 (subtilisin-like protease SBT1.2 OS=Cucurbita maxima OX=3661 GN=LOC111490865 PE=3 SV=1)

HSP 1 Score: 1431.0 bits (3703), Expect = 0.0e+00
Identity = 713/770 (92.60%), Postives = 744/770 (96.62%), Query Frame = 0

Query: 1   MDFNTQMAFLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQT 60
           MDFN+QM  LSTLFLCFLSLQVQAN STLQTYIIQLHP GLTSS F SK QWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQANTSTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFL 120
           LSAEEDSSSRLLYSYS AMEGFAAQLSETE+EYLKKLPDVVAVRAD+K+EIQTTYS KFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADKKYEIQTTYSPKFL 120

Query: 121 GLSSGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSS 180
           GLS G+QGVW+ S+MG+GAIVGVLDTGVWPESPSFSDS+MP IP+KWRGACQEGQDFNSS
Sbjct: 121 GLSLGSQGVWFNSAMGEGAIVGVLDTGVWPESPSFSDSRMPSIPQKWRGACQEGQDFNSS 180

Query: 181 ICNRKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGN 240
            CN+KLIGAKFFIKGHHVASS PSDV QEYVSPRDSHGHGTHTSSTAAGA+V EASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSPPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAP AHIAVYKVCWFSGCYSSDIVAAMD+AIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAI 360
           IAIGSFRAMQHGISV CAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAI+RLSNGE I
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360

Query: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEK 420
           YGESMYPGNKFK ATKELEVVYLTGGQMGGELCLKGSLPREQV+GK+VLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
           GQIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGF+EANRLKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFSEANRLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDGNKPAG 600
           NFTVMSGTSMACPHVSGI+ALIHSAHPKWTPAAIKSAIMTTA+VTDH+GKPILDG+KPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGH+NPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRV 720
           GFSLNYPSMSVVFK  TTSK VSRRLTNVGSPNSIYSVEV AP+GV+VRVKPR LVFK V
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTWKN 771
           NQSLNYKVWFMSEKG+EGRKVSS+EGHLTWVHSENGKY+VRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770

BLAST of Lag0033213 vs. ExPASy TrEMBL
Match: A0A5A7T534 (Subtilisin-like protease SBT1.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold154G00500 PE=3 SV=1)

HSP 1 Score: 1430.2 bits (3701), Expect = 0.0e+00
Identity = 716/770 (92.99%), Postives = 737/770 (95.71%), Query Frame = 0

Query: 1   MDFNTQMAFLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQT 60
           MDFNT+M FL  LFL FLSL VQ N STLQTYIIQLHP GL +S+FDSKLQWHLSFLEQT
Sbjct: 1   MDFNTRMGFL--LFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFL 120
           LSAEEDSSSRLLYSYS AMEGFAAQL+ETELEYLKKLPDVVAVR DRK++IQTTYSHKFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFL 120

Query: 121 GLSSGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSS 180
           GLS GTQGVW KSSMGQGAIVG+LDTGVWPESPSFSDSKMPPIP+KWRGACQEGQDFNSS
Sbjct: 121 GLSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSS 180

Query: 181 ICNRKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGN 240
            CNRKLIGAKFFIKGHHVASSLPSDV QEYVSPRDSHGHGTHTSSTAAGA+V +ASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAI 360
           IAIGSFRAMQHGISVVCAAGNNGP QSSVANVAPWITTIGA TLDRRFPAI+RLSNGEAI
Sbjct: 301 IAIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAI 360

Query: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEK 420
           YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPRE+VQGKMV+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
           GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPT LPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDGNKPAG 600
           NFTVMSGTSMACPHVSGI+ALIHSAHPKWTPAAIKSAIMTTADVTDHFGK ILDGNKPA 
Sbjct: 541 NFTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPAD 600

Query: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGHVNPTKAIDPGLVYDIKPYEY+IHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRV 720
           GF+LNYPSMSV+FK GTTSKMVSRRLTNVGS NSIY V+V APEGVRVRVKPR LVFK V
Sbjct: 661 GFTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHV 720

Query: 721 NQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTWKN 771
           NQSLNYKVWFMSEKG+EGRKV  TEG LTW+H EN KY+VRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of Lag0033213 vs. ExPASy TrEMBL
Match: A0A1S3BHA2 (subtilisin-like protease SBT1.2 OS=Cucumis melo OX=3656 GN=LOC103489836 PE=3 SV=1)

HSP 1 Score: 1430.2 bits (3701), Expect = 0.0e+00
Identity = 716/770 (92.99%), Postives = 737/770 (95.71%), Query Frame = 0

Query: 1   MDFNTQMAFLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQT 60
           MDFNT+M FL  LFL FLSL VQ N STLQTYIIQLHP GL +S+FDSKLQWHLSFLEQT
Sbjct: 1   MDFNTRMGFL--LFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFL 120
           LSAEEDSSSRLLYSYS AMEGFAAQL+ETELEYLKKLPDVVAVR DRK++IQTTYSHKFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFL 120

Query: 121 GLSSGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSS 180
           GLS GTQGVW KSSMGQGAIVG+LDTGVWPESPSFSDSKMPPIP+KWRGACQEGQDFNSS
Sbjct: 121 GLSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSS 180

Query: 181 ICNRKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGN 240
            CNRKLIGAKFFIKGHHVASSLPSDV QEYVSPRDSHGHGTHTSSTAAGA+V +ASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAI 360
           IAIGSFRAMQHGISVVCAAGNNGP QSSVANVAPWITTIGA TLDRRFPAI+RLSNGEAI
Sbjct: 301 IAIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAI 360

Query: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEK 420
           YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPRE+VQGKMV+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
           GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF
Sbjct: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPT LPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDGNKPAG 600
           NFTVMSGTSMACPHVSGI+ALIHSAHPKWTPAAIKSAIMTTADVTDHFGK ILDGNKPA 
Sbjct: 541 NFTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPAD 600

Query: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGHVNPTKAIDPGLVYDIKPYEY+IHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRV 720
           GF+LNYPSMSV+FK GTTSKMVSRRLTNVGS NSIY V+V APEGVRVRVKPR LVFK V
Sbjct: 661 GFTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHV 720

Query: 721 NQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTWKN 771
           NQSLNYKVWFMSEKG+EGRKV  TEG LTW+H EN KY+VRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of Lag0033213 vs. ExPASy TrEMBL
Match: A0A0A0LDY7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G610820 PE=3 SV=1)

HSP 1 Score: 1429.1 bits (3698), Expect = 0.0e+00
Identity = 713/770 (92.60%), Postives = 739/770 (95.97%), Query Frame = 0

Query: 1   MDFNTQMAFLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQT 60
           MDFN +M FL  LFLCFLSL VQ N STLQTYIIQLHP GL +S+FDSKLQWHLSFLEQ+
Sbjct: 1   MDFNARMGFL--LFLCFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQS 60

Query: 61  LSAEEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFL 120
           LSAEEDSSSRLLYSYS AMEGFAAQLSETELEYLK+LPDVVAVR DRK++IQTTYSHKFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKRLPDVVAVREDRKYQIQTTYSHKFL 120

Query: 121 GLSSGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSS 180
           GLS GTQG+  KSSMGQGAIVGVLDTGVWPESPSFSDSKMPP+P+KWRGACQEGQDFNSS
Sbjct: 121 GLSVGTQGLRQKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPVPQKWRGACQEGQDFNSS 180

Query: 181 ICNRKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGN 240
            CNRKLIGAKFFIKGHHVASSLPSDV QEYVSPRDSHGHGTHTSSTAAGA+V +ASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSLPSDVAQEYVSPRDSHGHGTHTSSTAAGASVADASVFGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAI 360
           IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGA TLDRRFPAI+RLSNGEAI
Sbjct: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGAGTLDRRFPAIIRLSNGEAI 360

Query: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEK 420
           YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPRE+VQGKMV+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
           GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDGNKPAG 600
           NFTVMSGTSMACPHVSGI+ALIHSAHPKWTPAAIKSAIMTTADVTDHFGK ILDGNKPA 
Sbjct: 541 NFTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPAD 600

Query: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGHVNPTKAIDPGLVYDIKPYEY+IHLCALGYTHSEIFIITHMNVSCHK+LQMNK
Sbjct: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKILQMNK 660

Query: 661 GFSLNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRV 720
           GF+LNYPS+SV+FK GTTSKMVSRRLTNVGS NSIY V+V APEGVRVRVKPR LVFK V
Sbjct: 661 GFTLNYPSISVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHV 720

Query: 721 NQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTWKN 771
           NQSLNYKVWFMSEKG+EGRKV  TEG LTW+H EN KY+VRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of Lag0033213 vs. ExPASy TrEMBL
Match: A0A6J1ES95 (subtilisin-like protease SBT1.2 OS=Cucurbita moschata OX=3662 GN=LOC111437268 PE=3 SV=1)

HSP 1 Score: 1426.4 bits (3691), Expect = 0.0e+00
Identity = 712/770 (92.47%), Postives = 741/770 (96.23%), Query Frame = 0

Query: 1   MDFNTQMAFLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQT 60
           MDFN+QM  LSTLFLCFLSLQVQAN  TLQTYIIQLHP GLTSS F SK QWHLSFLEQT
Sbjct: 1   MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60

Query: 61  LSAEEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFL 120
           LSAEEDSSSRLLYSYS AMEGFAAQLSETE+EYLKKLPDVVAVRADRK+EIQTTYS KFL
Sbjct: 61  LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120

Query: 121 GLSSGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSS 180
           GLS GTQGVW+ SSMG+GAIVGVLDTGVWPESPSFSDS+MP IP+KWRGACQEGQDFNSS
Sbjct: 121 GLSLGTQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPSIPQKWRGACQEGQDFNSS 180

Query: 181 ICNRKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGN 240
            CN+KLIGAKFFIKGHHVASS PSDV QEYVSPRDSHGHGTHTSSTAAGA+V EASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSPPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAP AHIAVYKVCWFSGCYSSDIVAAMD+AIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300

Query: 301 IAIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAI 360
           IAIGSFRAMQHGISV CAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAI+RLSNGE I
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360

Query: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEK 420
           YGESMYPGNKFK ATKELEVVYLTGGQMGGELCLKGSLPREQV+GK+VLCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420

Query: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
           GQIVKESGGAAMILAN+ INLEEDLVDVHVLPATLIGFAEAN+LKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANQLKAYMNTTSNPKARIQF 480

Query: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
           GGTVIGRSRAPSVAQFSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540

Query: 541 NFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDGNKPAG 600
           NFTVMSGTSMACPHVSGI+ALIHSAHPKWTPAAIKSAIMTTA+VTDH+GKPILDG+KPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600

Query: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAGH+NPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660

Query: 661 GFSLNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRV 720
           GFSLNYPSMSVVFK  TTSK VSRRLTNVGSPNSIYSVEV AP+GV+VRVKPR LVFK V
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720

Query: 721 NQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTWKN 771
           NQSLNYKVWFMSEKG+EG+ VSS+EGHLTWVHSENGKY+VRSPIVVTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770

BLAST of Lag0033213 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 1059.3 bits (2738), Expect = 1.5e-309
Identity = 519/767 (67.67%), Postives = 634/767 (82.66%), Query Frame = 0

Query: 14  FLCFLSLQVQANASTL---QTYIIQLHPLGLTSSLFDSKLQWHLSFLEQTL----SAEED 73
           FLC + L   +++S +   QTYI+QLHP   T+  F SK  WHLSFL++ +      EE+
Sbjct: 7   FLCIIFLLFCSSSSEILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 66

Query: 74  SSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFLGLSS-G 133
            SSRLLYSY  A+EGFAAQL+E+E E L+  P+VVAVR D   ++QTTYS+KFLGL   G
Sbjct: 67  PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 126

Query: 134 TQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSSICNRK 193
             GVW KS  GQG I+GVLDTGVWPESPSF D+ MP IP+KW+G CQEG+ F+SS CNRK
Sbjct: 127 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 186

Query: 194 LIGAKFFIKGHHVASSLPS--DVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGNGAG 253
           LIGA+FFI+GH VA+S     ++P+EY+S RDS GHGTHT+ST  G++V  A+V GNGAG
Sbjct: 187 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 246

Query: 254 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAI 313
           VA+GMAPGAHIAVYKVCWF+GCYSSDI+AA+D AI+D VD+LSLSLGGFP+P +DD+IAI
Sbjct: 247 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 306

Query: 314 GSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAIYGE 373
           G+FRAM+ GISV+CAAGNNGPI+SSVAN APW++TIGA TLDRRFPA++RL+NG+ +YGE
Sbjct: 307 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 366

Query: 374 SMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEKGQI 433
           S+YPG   K A +E+EV+Y+TGG  G E CL+GSLPRE+++GKMV+CDRGVNGRSEKG+ 
Sbjct: 367 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 426

Query: 434 VKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGT 493
           VKE+GG AMILAN+EIN EED +DVH+LPATLIG+ E+  LKAY+N T  PKARI FGGT
Sbjct: 427 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 486

Query: 494 VIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNFT 553
           VIGRSRAP VAQFS+RGPSL+NPS LKPD+IAPGVNIIAAWPQNLGPTGLP DSRR NFT
Sbjct: 487 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 546

Query: 554 VMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDGNKPAGVFA 613
           VMSGTSM+CPHVSGI+ALI SA+P W+PAAIKSA+MTTAD+ D  GK I DGNKPAGVFA
Sbjct: 547 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 606

Query: 614 MGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNKGFS 673
           +GAGHVNP KAI+PGLVY+I+P +Y+ +LC LG+T S+I  ITH NVSC+ +L+ N GFS
Sbjct: 607 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 666

Query: 674 LNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRVNQS 733
           LNYPS++V+FKRG T++M++RR+TNVGSPNSIYSV V APEG++V V P+ LVFK V+Q+
Sbjct: 667 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 726

Query: 734 LNYKVWFMSEKGREGRKVSS-TEGHLTWVHSENGKYRVRSPIVVTWK 770
           L+Y+VWF+ +K   G KV+S  +G LTWV+S N   RVRSPI VT K
Sbjct: 727 LSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPISVTLK 773

BLAST of Lag0033213 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 651.0 bits (1678), Expect = 1.2e-186
Identity = 362/765 (47.32%), Postives = 484/765 (63.27%), Query Frame = 0

Query: 12  TLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQTLSAEEDSSSRL 71
           T+   FL L +   A   +TYII+++      S F +   W+ S L        +S S L
Sbjct: 12  TIITTFLFLLLHTTAK--KTYIIRVNHSDKPES-FLTHHDWYTSQL--------NSESSL 71

Query: 72  LYSYSKAMEGFAAQLSETELE-YLKKLPDVVAVRADRKHEIQTTYSHKFLGLSSGTQGVW 131
           LY+Y+ +  GF+A L  TE +  L     ++ +  D  + + TT + +FLGL+S   GV 
Sbjct: 72  LYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNS-EFGVH 131

Query: 132 YKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSSICNRKLIGAK 191
              S   G I+GVLDTGVWPES SF D+ MP IP KW+G C+ G DF+S +CN+KLIGA+
Sbjct: 132 DLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGAR 191

Query: 192 FFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGNGAGVAQGMAP 251
            F KG  +AS       +E VSPRD  GHGTHTS+TAAG+AV  AS  G  AG A+GMA 
Sbjct: 192 SFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMAT 251

Query: 252 GAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQ 311
            A +A YKVCW +GC+ SDI+AAMD AI DGVD+LSLSLGG   P++ D+IAIG+F AM+
Sbjct: 252 RARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAME 311

Query: 312 HGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAIYGESMYPGNK 371
            G+ V C+AGN+GP ++SVANVAPW+ T+GA TLDR FPA   L NG+ + G S+Y G  
Sbjct: 312 RGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG-- 371

Query: 372 FKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEKGQIVKESGGA 431
               TK LE+VY  G      LCL GSL    V+GK+V+CDRGVN R EKG +V+++GG 
Sbjct: 372 VGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGL 431

Query: 432 AMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVIGRSRA 491
            MI+AN+  + EE + D H+LPA  +G    + L+ Y+ + + P A + F GTV+    +
Sbjct: 432 GMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS 491

Query: 492 PSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNFTVMSGTSM 551
           P VA FSSRGP+   P  LKPDVI PGVNI+A W   +GPTGL +DSRR+ F +MSGTSM
Sbjct: 492 PVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSM 551

Query: 552 ACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILDG--NKPAGVFAMGAGH 611
           +CPH+SG++ L+ +AHP+W+P+AIKSA+MTTA V D+   P+ D   N  +  +A G+GH
Sbjct: 552 SCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGH 611

Query: 612 VNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIF-IITHMNVSCHKVLQMNKGFSLNYP 671
           V+P KA+ PGLVYDI   EY+  LC+L YT   I  I+   +V+C K  + +    LNYP
Sbjct: 612 VDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFSDPGQLNYP 671

Query: 672 SMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMAPEGVRVRVKPRHLVFKRVNQSLNYK 731
           S SV+F  G      +R +TNVG+ +S+Y V V     V + VKP  L FK V +   Y 
Sbjct: 672 SFSVLF-GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYT 731

Query: 732 VWFMSEKGREGRKVSSTE----GHLTWVHSENGKYRVRSPIVVTW 769
           V F+S+KG     VS T     G +TW    N ++ VRSP+  +W
Sbjct: 732 VTFVSKKG-----VSMTNKAEFGSITW---SNPQHEVRSPVAFSW 751

BLAST of Lag0033213 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 634.0 bits (1634), Expect = 1.5e-181
Identity = 352/773 (45.54%), Postives = 489/773 (63.26%), Query Frame = 0

Query: 8   AFLST----LFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQTLSA 67
           +FLS+    L LC     V +++S   TYI+ +    + SS FD    W+ S L      
Sbjct: 4   SFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSS-FDLHSNWYDSSLRSI--- 63

Query: 68  EEDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFLGLS 127
               S+ LLY+Y  A+ GF+ +L++ E + L   P V++V  + ++E+ TT +  FLGL 
Sbjct: 64  --SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD 123

Query: 128 SGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSSICN 187
             T  ++ ++      +VGVLDTGVWPES S+SD    PIP  W+G C+ G +F +S+CN
Sbjct: 124 EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCN 183

Query: 188 RKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGNGAG 247
           RKLIGA+FF +G+  ++  P D  +E  SPRD  GHGTHTSSTAAG+ V  AS+ G  +G
Sbjct: 184 RKLIGARFFARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 243

Query: 248 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAI 307
            A+GMAP A +AVYKVCW  GC+SSDI+AA+D AI D V++LS+SLGG    ++ D +AI
Sbjct: 244 TARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAI 303

Query: 308 GSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAIYGE 367
           G+F AM+ GI V C+AGN GP  SS++NVAPWITT+GA TLDR FPA+  L NG+   G 
Sbjct: 304 GAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGV 363

Query: 368 SMYPGNKFKQATKELEVVYL--TGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEKG 427
           S++ G       K L  +Y         G LC+ G+L  E+V+GK+V+CDRG+N R +KG
Sbjct: 364 SLFKGEALPD--KLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKG 423

Query: 428 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 487
            +VK +GG  MILAN+  N EE + D H+LPAT +G    + ++ Y+ T  NP A I   
Sbjct: 424 DVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISIL 483

Query: 488 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSN 547
           GTV+G   +P VA FSSRGP+   P+ LKPD+IAPGVNI+AAW    GPTGL  DSRR  
Sbjct: 484 GTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVE 543

Query: 548 FTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILD--GNKPA 607
           F ++SGTSM+CPHVSG++AL+ S HP+W+PAAI+SA+MTTA  T   GKP+LD    KP+
Sbjct: 544 FNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPS 603

Query: 608 GVFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMN 667
             F  GAGHV+PT A +PGL+YD+   +Y+  LCAL YT  +I  ++  N +C      +
Sbjct: 604 TPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTC----DPS 663

Query: 668 KGFS---LNYPSMSVVFKRGTTSKMVSRRLTNVGSPNSIYSVEVMA-PEGVRVRVKPRHL 727
           K +S   LNYPS +V    G  +   +R +T+VG   + YSV+V +   GV++ V+P  L
Sbjct: 664 KSYSVADLNYPSFAVNVD-GVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 723

Query: 728 VFKRVNQSLNYKVWFMSEKGREGRKVSSTEGHLTWVHSENGKYRVRSPIVVTW 769
            FK  N+  +Y V F  +  +     S++ G + W    +GK+ V SP+ ++W
Sbjct: 724 NFKEANEKKSYTVTFTVDSSKPSG--SNSFGSIEW---SDGKHVVGSPVAISW 756

BLAST of Lag0033213 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 629.4 bits (1622), Expect = 3.8e-180
Identity = 346/781 (44.30%), Postives = 490/781 (62.74%), Query Frame = 0

Query: 7   MAFLSTLF--LCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQTLSAE 66
           MAF    F  L   S    A++S   TYI+ +       S+F +   W+ S    +L++ 
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDH-EAKPSIFPTHFHWYTS----SLASL 60

Query: 67  EDSSSRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFLGL-S 126
             S   ++++Y     GF+A+L+  +   L   P V++V  ++   + TT S +FLGL S
Sbjct: 61  TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRS 120

Query: 127 SGTQGVWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSSICN 186
           +   G+  +S  G   ++GV+DTGVWPE PSF D  + P+P KW+G C   QDF  S CN
Sbjct: 121 TDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACN 180

Query: 187 RKLIGAKFFIKGHHVASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGNGAG 246
           RKL+GA+FF  G+   +   ++   E+ SPRDS GHGTHT+S +AG  V  AS  G   G
Sbjct: 181 RKLVGARFFCGGYEATNGKMNET-TEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHG 240

Query: 247 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAI 306
           VA GMAP A +A YKVCW SGCY SDI+AA D+A+ DGVD++SLS+GG  +P++ D+IAI
Sbjct: 241 VAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAI 300

Query: 307 GSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAIYGE 366
           G+F A+  GI V  +AGN GP   +V NVAPW+TT+GA T+DR FPA ++L NG+ I G 
Sbjct: 301 GAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGV 360

Query: 367 SMYPGNKFKQATKELEVVYLTGGQMGGE-----LCLKGSLPREQVQGKMVLCDRGVNGRS 426
           S+Y G       +   +VY  G  +GG+     LCL+GSL    V+GK+VLCDRG+N R+
Sbjct: 361 SVYGGPGLDPG-RMYPLVY-GGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRA 420

Query: 427 EKGQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYIN------TTT 486
            KG+IV+++GG  MI+AN   + E  + D HVLPAT +G +  + ++ YI+      ++ 
Sbjct: 421 TKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSK 480

Query: 487 NPKARIQFGGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTG 546
           +P A I F GT +G   AP VA FS+RGP+   P  LKPDVIAPG+NI+AAWP  +GP+G
Sbjct: 481 HPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 540

Query: 547 LPEDSRRSNFTVMSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPI 606
           +  D+RR+ F ++SGTSMACPHVSG++AL+ +AHP W+PAAI+SA++TTA   D+ G+P+
Sbjct: 541 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 600

Query: 607 LDGN--KPAGVFAMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNV 666
           +D +    + V   G+GHV+PTKA+DPGLVYDI  Y+Y+  LC   YT + I  IT    
Sbjct: 601 MDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 660

Query: 667 SCHKVLQMNKGFSLNYPSMSVVFKRGTTSKMVS---RRLTNVGSPNSIYSVEVMAPEGVR 726
            C    +     +LNYPS SVVF++   SKM +   R +TNVG  +S+Y +++  P G  
Sbjct: 661 DCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTT 720

Query: 727 VRVKPRHLVFKRVNQSLNYKVWFMSEKGREGRKVSSTE-GHLTWVHSENGKYRVRSPIVV 768
           V V+P  L F+RV Q L++ V   + + +     ++ E GH+ W    +GK  V SP+VV
Sbjct: 721 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVW---SDGKRNVTSPLVV 770

BLAST of Lag0033213 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 618.6 bits (1594), Expect = 6.7e-177
Identity = 354/774 (45.74%), Postives = 478/774 (61.76%), Query Frame = 0

Query: 9   FLSTLFLCFLSLQVQANASTLQTYIIQLHPLGLTSSLFDSKLQWHLSFLEQTLSAEEDSS 68
           F+  L LCF S    +++  L++YI+ +       SLF S   WH+S L    S+ + ++
Sbjct: 11  FVFPLLLCFFS-PSSSSSDGLESYIVHVQ-RSHKPSLFSSHNNWHVSLLRSLPSSPQPAT 70

Query: 69  SRLLYSYSKAMEGFAAQLSETELEYLKKLPDVVAVRADRKHEIQTTYSHKFLGLSSGTQG 128
             LLYSYS+A+ GF+A+LS  +   L++ P V++V  D+  EI TT++  FLG S  + G
Sbjct: 71  --LLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNS-G 130

Query: 129 VWYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPKKWRGACQEGQDFNSSICNRKLIG 188
           +W  S+ G+  IVGVLDTG+WPE PSFSDS + PIP  W+G C+ G DF +S CNRKLIG
Sbjct: 131 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 190

Query: 189 AKFFIKGHHV-ASSLPSDVPQEYVSPRDSHGHGTHTSSTAAGAAVVEASVFGNGAGVAQG 248
           A+ F +G+    +       +E  SPRD+ GHGTHT+STAAG+ V  AS++    G A G
Sbjct: 191 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 250

Query: 249 MAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLG--GFPLPFFDDSIAIGS 308
           MA  A IA YK+CW  GCY SDI+AAMD A+ DGV ++SLS+G  G    +  DSIAIG+
Sbjct: 251 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 310

Query: 309 FRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAILRLSNGEAIYGESM 368
           F A +HGI V C+AGN+GP   +  N+APWI T+GAST+DR F A     +G+   G S+
Sbjct: 311 FGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSL 370

Query: 369 YPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVLCDRGVNGRSEKGQIVK 428
           Y G     +  +L +VY   G  G  LC  G L    V+GK+VLCDRG N R EKG  VK
Sbjct: 371 YAGESLPDS--QLSLVY--SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVK 430

Query: 429 ESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVI 488
            +GGA MILAN+  + EE   D H++PAT++G    ++++ YI T+ +P A+I F GT+I
Sbjct: 431 LAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLI 490

Query: 489 GRS-RAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSNFTV 548
           G S  +P VA FSSRGP+   P  LKPDVIAPGVNI+A W   +GPT L  D RR  F +
Sbjct: 491 GPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNI 550

Query: 549 MSGTSMACPHVSGISALIHSAHPKWTPAAIKSAIMTTADVTDHFGKPILD--GNKPAGVF 608
           +SGTSM+CPHVSG++AL+  AHP W+PAAIKSA++TTA   ++ G+PI D    K +  F
Sbjct: 551 ISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSF 610

Query: 609 AMGAGHVNPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITH---MNVSCHKVLQMN 668
             GAGHV+P KA++PGLVYDI+  EYV  LCA+GY    I +      +  +C +  ++ 
Sbjct: 611 IHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDAC-ETSKLR 670

Query: 669 KGFSLNYPSMSVVFKRGTTSKMVSRRLTNVGS-PNSIYSVEVMAPEGVRVRVKPRHLVFK 728
               LNYPS SVVF          R + NVGS  +++Y V V +P  V + V P  L F 
Sbjct: 671 TAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFS 730

Query: 729 RVNQSLNYKVWFMSEKGREGRKVSSTEGH----LTWVHSENGKYRVRSPIVVTW 769
           +    L Y+V F S     G  V S  GH    + W    +G++ V+SP+ V W
Sbjct: 731 KEKSVLEYEVTFKSVV--LGGGVGSVPGHEFGSIEWT---DGEHVVKSPVAVQW 769