Lag0033175 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033175
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPectate lyase
Locationchr11: 41447143 .. 41452011 (+)
RNA-Seq ExpressionLag0033175
SyntenyLag0033175
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTTTCTTCTCTTAGAGTATTACGGTTTTCATCGGCTTCTTCGTTGCTCTTCCTTCTTCTCAACGTCCTCTTCGTTCATTCCAGGTAAATCGCCTCGATCCTCTGCGATTTCTGAGATCAATGCCGTCGCCGACGGTCTAAATTAGTATAGTTTGTGATTTTGAGAATGTTGATTTAGATCGTTCTCATTTTTTGTTTCTATTTTCACATTGTTTGATAGTTTTGCTGGAGATGAGAGATTGCAGAGCTTCAATTCTTCATCAATGGCGGACGGGTCAGTATCGGAACTGAAGCTTTTGTTTGTTTGTTTTTCTTTTTTTTTTTTTTTTGGGAAGATCTTTTCTGATGGCCGAGCGCGTAATTTAGACCGTCAGATGTATCAAACGGTTCAGAAAAAAGGCAATTTTGAGATCGGATCTGGAAAATCTGGGCCGGTTTTTCATTTTACAGTCCTTATTAATTACTTTCTTTGACTCTTCCTTTGACCATTTTGCCCTTCAACATTCCATTTCAACTCTCACCGAATTTTGGACCTTTTGGTCAATGGCTAATTTCACTTCCACGTGTACTGTTTTTTTTTTTTTTTGTTTCACCTCTGAATTAAATTCAATAATTTTTTTTTTGAAGTACAACAAATTAATTTTTTTTTACAAACATGTGAGATAGAGAATTCGAACCACAAATCTCTCGGTCATAAGTACAAGCTTTATGCCAACAAGATCATAACTCAACTGGCATAAAATTTGTACGGATAACCACGAGGTTTGTGGTTCAAATTCTCGCACCCCATATTGTCAAAAAAAAAAAAAAAAGTACAAGTTTTATGCCAATGCTCATTGCTCAAAGTGAGCCTAGCTTAGCAGTAATTGACATGTATTCTAGGTCAGATGTTCCAATTTTCACCTCAAATGTTGATGTATTAAAAAAAAGAAATAGAAATCTTTATTTATGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAAGATCTTTATTTATGAGCTACAATTAAAATATATGAAACAATACTCCACCGACCAAAAGTTTAATATTATTATTAATTTTCTTTTTTGTTTCGATAAGTAAATTAAGATATTTTGATTATGTAATTTAAATGTTCTAATTTATTTGAATCTTTGTTTAATTGAAATTTTCAGGTATGTTAAGAACGAGCATGCTGTTGATAATCCAGAGGAAATTGCATCAATGGTTGATTTGTAAGATTCCCCTCTCTCTCTCTGATATTTATTTCTTAGGCTAAATGAAGAATTAAAAAAAAGAAAGAATAATAGTTAATAAAAAAAAAAATTCTTTTTGAGTAGCATGTGCATTTTTTTCCTCCAAATATGTTGAGTTTCGATGGAGGGCTTTAATTTGAAGTTTTAAATGAATGTTAGAATGAGGAAATTCTCATTATTTTAAGTATTGAAAAAGACTTCTAATTGACGTTTCTTTTTCTCGTAATATATGGACTCGAAGAGAGAGATTTCTAATACTATGGTGGTATAAATTTTTTTTTCATACTATTTTCGTTTATTCTTTGTCATAAAGAAGTATTTAAGAATCATATCAGACTTATATAATAAAATATTAAATAATTAAATGTTCATTAAATGACGTGTTAAATGCTAAATTAAGAGTATATACTAATGTAGTATTAGAAAATTTTCCATGTAACACAGTGGGTAAGATTTTATTTTATTAATGTTGAAATTTATATTATATAATATGATTCGGAGGGGCCCAACTACCACGTTCCTTGCTACTGCAATTTCAATCTCTTTCACACATGGCCATGTGGCGAGATCCACCTGTCACGCTCATAATTTATTTCTTGTCTCTTCCCTTATCCAAAATTTGGAAATAGATTTATAAATATTGTTTAAATAATAAAAAATTATATATTTACATATAGCCAGATTTTGGATTACCCCTTATCTCTTGTAAAATGTATCCTTTCATATTTGCATTGCCCAAAAGCATCTCATTATTTCAATCTTCAAAAAAGGTTCCTTGTTGCCATTCCCTCCTCTCACCCTAGGAGAATTATTGAACGAAGCTATCATAACCGTTGAATGTAGATTTGAACATCTGCGTGTCTTTGACGATAGGATACAAATTCAAAATATTTTTTACAATTTCTCTCTTATAGTTCCAGTGGTGTGAAATTACCCGCCATAATATAACAACCTCTTCCAATAATTTATCCTAATTTTTCATCCTTTTCAGCCAAGTAACTTTTTAGAATAGACCATGCACTTCAATCTCTCACAAGCAATGTCTCCATTAGTATGAGATATTTTGAGTGAAAAAGTAAAATCCACGAAGGCTTAGTTTAAAGTGGACAATAACATATCACTGTAGAAATGTGTGGAAGGTCGGTTTATCCTAATAAGATTTGAATTAGTGTACTTTCTCTCTCACTCTAAAGTACTAATCCACATGAATGTCATTATCATAGATCATTGTTTTGGAATCAAACGCATTTATATTAGCAATGCAATGCCATTTCATTGCGTGAGTCCCACTTCACATTTACTCAAAAAACAATCACTATAGTTAAGAGAGGAGTATGGCATATAAATGTTTATGTCTTTTTTGCCTTCGAAAATCACCGGTCACTAGCTAACTATATTCTCAGATCTGATTTCTCACCAGAAATCTCGTTTTTCTCTTTCTTTTTTCGGTTGAATTTATTGCAGCAAATCATGCAGGCGGCAGGCAGCCAGGCAGCTGCCTAGCATGCAGAACTATACTAATCAATAAATGAGGCCACCTTTGCATTTATAACTCCTACACCGACACATCAAATTTTAGTCAAATAAAGGAAAAAAGGAATGAATTGGCTCTCTCTTTCATCTGTAGCATTAAATTTTCCCAGCAAATTTTACAAGTTCTTTTTTATAGTTAACGCCAGTTTCCCGTATGATCCGTATCCACCCAACCCATTGAATGTGAGACTCCAAAATTAATCCTTTTTTAGTTTAACGTTGGAAGAATTCTAACTGTGGAATGAGCTGATTCAAATTTCTGACCTTCTGACCTGTTGATCGAGAATATATACTTTTGAACAGTTGAACTATACTCAAGTTAGTCAAGTTATAGATCTCATCTGGGTTGAGAGATGAGATTTAGATTTAAATTCATAGGCATAAGTAATGCAGTTACTGATTTACGTGTGTTTGGAAATTTCCTAGGAGGATCCGCAATAGCACAGAGAGGAGGAATTTGGGGTTCTTCTCCTGCGGGACAGGAAATCCAATTGATGATTGTTGGCGCTGTGACCCGCGTTGGCAACTTCGCCGAAAGCACTTGGCAGATTGCGGCATTGGGTTTGGTCGTAATGCTGTCGGTGGCCGTGATGGGCGATACTACGTCGTCTCTGATCCCGGCGACGATGACCCTGTCAATCCCAGACCAGGCACTCTTCGTCATGCTGTGATTCAAGACAGGCCACTGTGGATTGTGTTCAAAAGAGACATGGTCATCACTCTGAAGCAAGAACTGATCATGAACAGCTTCAAGACAATCGATGCCCGTGGTGCCAATGTCCACATTGCTTATGGGGCTTGCATTACAATCCAATTCATTACCAATGTCATTATTCATGGCCTGCACATTCATGACTGCAAGCCAACTGGGAACGCCATGGTTCGAAGCTCCCCTAGCCACTATGGATGGAGGACAATGGCTGATGGCGATGGCATTTCCATTTTTGGGTCGAGTCATATTTGGATTGACCATAATTCTCTTTCTAGCTGTGCTGATGGGCTCATTGATGCTGTGATGGGATCTACTGCCATCACCATTTCTAACAACTACTTCACCCACCACAATGAGGTAAAGCTACTTTCATCCAATGATCCAAAGAATGTATGTATTGCAAATTGGAGTTTGGTCTGAAATTGGCTGAGAATGTTACACAACAGGTGATGCTTTTGGGACATAGTGACTCTTATGTAAGAGACAAGCAAATGCAAGTGACCATTGCTTATAACCATTTTGGTGAAGGTCTCATCCAAAGAATGCCAAGGTAGGTGCTCTTCTCTCTCTTTCTTTTTCTTTGGGAAAACTTTCGTTATTACTTTAGTTTGGATACTCCTTTCATTCATTGTCTGTCACGACAGCATCATTTTAAAAATGATGTCATGTCATGTTCACATACTCCTTTCATTAATTATACTCCTTTCATTAATTATCTGTCACGTCAACATCATTTTAAAATGAAATAGGGTCCACCAACATTAAAAGAAAAGACAAAATTATAGTGCAAACAAAGGGAGTAAATAATCTCGTAGTACTAAAAATTTTCCCGATGTACCCTAACCTGAATAAAAATTAACTGTTGGGACCCGATCATTTAATGTTGCAGGTGTAGACATGGGTATTTCCATGTAGTAAACAATGACTACACTCATTGGGTAATGTATGCCATCGGTGGAAGTGCTGATCCTACCATTAATAGCCAAGGCAACAGATATCTTGCCCCTGCCAACCCTTTTGCCAAAGAGGTATCTTTCCTCTCTCTCTAGATCTCAATCCTGTTTGAAACAGTACAGAAATACAGATCAAGATGATGTTCTTATAACTTATAAGCAATGGCTGATTAGTTCTAATGGTTAATGTGTGCAGGTCACAAAGAGAGTCGACACGTGGAATGGCGTATGGAAGCACTGGAATTGGAGATCAGAGGGAGATCTTCTGCTAAATGGAGCCTATTTCACTCCATCAGGTGCTGGTGCAGCAGCCAGTTATGCAAGAGCCTCAAGCTTGGGAGCCAAATCCTCTTCCTTGGTGGGCTCCATTACTTCAAGTGCAGGTGCCCTTTCTTGTCGTAGGGGATACCGTTGTTAA

mRNA sequence

ATGGCGGTTTCTTCTCTTAGAGTATTACGGTTTTCATCGGCTTCTTCGTTGCTCTTCCTTCTTCTCAACGTCCTCTTCGTTCATTCCAGTTTTGCTGGAGATGAGAGATTGCAGAGCTTCAATTCTTCATCAATGGCGGACGGGTATGTTAAGAACGAGCATGCTGTTGATAATCCAGAGGAAATTGCATCAATGGTTGATTTGAGGATCCGCAATAGCACAGAGAGGAGGAATTTGGGGTTCTTCTCCTGCGGGACAGGAAATCCAATTGATGATTGTTGGCGCTGTGACCCGCGTTGGCAACTTCGCCGAAAGCACTTGGCAGATTGCGGCATTGGGTTTGGTCGTAATGCTGTCGGTGGCCGTGATGGGCGATACTACGTCGTCTCTGATCCCGGCGACGATGACCCTGTCAATCCCAGACCAGGCACTCTTCGTCATGCTGTGATTCAAGACAGGCCACTGTGGATTGTGTTCAAAAGAGACATGGTCATCACTCTGAAGCAAGAACTGATCATGAACAGCTTCAAGACAATCGATGCCCGTGGTGCCAATGTCCACATTGCTTATGGGGCTTGCATTACAATCCAATTCATTACCAATGTCATTATTCATGGCCTGCACATTCATGACTGCAAGCCAACTGGGAACGCCATGGTTCGAAGCTCCCCTAGCCACTATGGATGGAGGACAATGGCTGATGGCGATGGCATTTCCATTTTTGGGTCGAGTCATATTTGGATTGACCATAATTCTCTTTCTAGCTGTGCTGATGGGCTCATTGATGCTGTGATGGGATCTACTGCCATCACCATTTCTAACAACTACTTCACCCACCACAATGAGGTGATGCTTTTGGGACATAGTGACTCTTATGTAAGAGACAAGCAAATGCAAGTGACCATTGCTTATAACCATTTTGGTGAAGGTCTCATCCAAAGAATGCCAAGGTGTAGACATGGGTATTTCCATGTAGTAAACAATGACTACACTCATTGGGTAATGTATGCCATCGGTGGAAGTGCTGATCCTACCATTAATAGCCAAGGCAACAGATATCTTGCCCCTGCCAACCCTTTTGCCAAAGAGGTCACAAAGAGAGTCGACACGTGGAATGGCGTATGGAAGCACTGGAATTGGAGATCAGAGGGAGATCTTCTGCTAAATGGAGCCTATTTCACTCCATCAGGTGCTGGTGCAGCAGCCAGTTATGCAAGAGCCTCAAGCTTGGGAGCCAAATCCTCTTCCTTGGTGGGCTCCATTACTTCAAGTGCAGGTGCCCTTTCTTGTCGTAGGGGATACCGTTGTTAA

Coding sequence (CDS)

ATGGCGGTTTCTTCTCTTAGAGTATTACGGTTTTCATCGGCTTCTTCGTTGCTCTTCCTTCTTCTCAACGTCCTCTTCGTTCATTCCAGTTTTGCTGGAGATGAGAGATTGCAGAGCTTCAATTCTTCATCAATGGCGGACGGGTATGTTAAGAACGAGCATGCTGTTGATAATCCAGAGGAAATTGCATCAATGGTTGATTTGAGGATCCGCAATAGCACAGAGAGGAGGAATTTGGGGTTCTTCTCCTGCGGGACAGGAAATCCAATTGATGATTGTTGGCGCTGTGACCCGCGTTGGCAACTTCGCCGAAAGCACTTGGCAGATTGCGGCATTGGGTTTGGTCGTAATGCTGTCGGTGGCCGTGATGGGCGATACTACGTCGTCTCTGATCCCGGCGACGATGACCCTGTCAATCCCAGACCAGGCACTCTTCGTCATGCTGTGATTCAAGACAGGCCACTGTGGATTGTGTTCAAAAGAGACATGGTCATCACTCTGAAGCAAGAACTGATCATGAACAGCTTCAAGACAATCGATGCCCGTGGTGCCAATGTCCACATTGCTTATGGGGCTTGCATTACAATCCAATTCATTACCAATGTCATTATTCATGGCCTGCACATTCATGACTGCAAGCCAACTGGGAACGCCATGGTTCGAAGCTCCCCTAGCCACTATGGATGGAGGACAATGGCTGATGGCGATGGCATTTCCATTTTTGGGTCGAGTCATATTTGGATTGACCATAATTCTCTTTCTAGCTGTGCTGATGGGCTCATTGATGCTGTGATGGGATCTACTGCCATCACCATTTCTAACAACTACTTCACCCACCACAATGAGGTGATGCTTTTGGGACATAGTGACTCTTATGTAAGAGACAAGCAAATGCAAGTGACCATTGCTTATAACCATTTTGGTGAAGGTCTCATCCAAAGAATGCCAAGGTGTAGACATGGGTATTTCCATGTAGTAAACAATGACTACACTCATTGGGTAATGTATGCCATCGGTGGAAGTGCTGATCCTACCATTAATAGCCAAGGCAACAGATATCTTGCCCCTGCCAACCCTTTTGCCAAAGAGGTCACAAAGAGAGTCGACACGTGGAATGGCGTATGGAAGCACTGGAATTGGAGATCAGAGGGAGATCTTCTGCTAAATGGAGCCTATTTCACTCCATCAGGTGCTGGTGCAGCAGCCAGTTATGCAAGAGCCTCAAGCTTGGGAGCCAAATCCTCTTCCTTGGTGGGCTCCATTACTTCAAGTGCAGGTGCCCTTTCTTGTCGTAGGGGATACCGTTGTTAA

Protein sequence

MAVSSLRVLRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSSSMADGYVKNEHAVDNPEEIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNAVGGRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGSITSSAGALSCRRGYRC
Homology
BLAST of Lag0033175 vs. NCBI nr
Match: KAG6606330.1 (putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 860.1 bits (2221), Expect = 8.0e-246
Identity = 419/436 (96.10%), Postives = 427/436 (97.94%), Query Frame = 0

Query: 1   MAVSSLRVLRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSSSMADGYVKNEHAVDNPE 60
           MAV SLR L FS+ASSLLFLLL++LFVHSSFAGDERLQSFNSSSMAD  VKNEHAVDNPE
Sbjct: 1   MAV-SLR-LHFSTASSLLFLLLHILFVHSSFAGDERLQSFNSSSMADWNVKNEHAVDNPE 60

Query: 61  EIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNAVG 120
           EIASMVD  IRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLA+CGIGFGRNAVG
Sbjct: 61  EIASMVDSSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLANCGIGFGRNAVG 120

Query: 121 GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID 180
           GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID
Sbjct: 121 GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID 180

Query: 181 ARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISI 240
            RGANVHIAYGACITIQF+TNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDG+SI
Sbjct: 181 GRGANVHIAYGACITIQFVTNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGVSI 240

Query: 241 FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQV 300
           FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEV+LLGHSDSYVRDKQMQV
Sbjct: 241 FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVILLGHSDSYVRDKQMQV 300

Query: 301 TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF 360
           TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF
Sbjct: 301 TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF 360

Query: 361 AKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGS 420
           AKEVTKRVDTWNG WKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVG+
Sbjct: 361 AKEVTKRVDTWNGEWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGT 420

Query: 421 ITSSAGALSCRRGYRC 437
           ITSSAGALSCRRGYRC
Sbjct: 421 ITSSAGALSCRRGYRC 434

BLAST of Lag0033175 vs. NCBI nr
Match: XP_022996350.1 (probable pectate lyase 8 [Cucurbita maxima])

HSP 1 Score: 859.4 bits (2219), Expect = 1.4e-245
Identity = 419/436 (96.10%), Postives = 426/436 (97.71%), Query Frame = 0

Query: 1   MAVSSLRVLRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSSSMADGYVKNEHAVDNPE 60
           MAV SLR L FS ASSLLFLLL++LFVHSSFAGDERLQSFNSSSMAD  VKNEHAVDNPE
Sbjct: 1   MAV-SLR-LHFSMASSLLFLLLHILFVHSSFAGDERLQSFNSSSMADWNVKNEHAVDNPE 60

Query: 61  EIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNAVG 120
           EIASMVD  IRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLA+CGIGFGRNAVG
Sbjct: 61  EIASMVDSSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLANCGIGFGRNAVG 120

Query: 121 GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID 180
           GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID
Sbjct: 121 GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID 180

Query: 181 ARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISI 240
            RGANVHIAYGACITIQF+TNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDG+SI
Sbjct: 181 GRGANVHIAYGACITIQFVTNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGVSI 240

Query: 241 FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQV 300
           FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEV+LLGHSDSYVRDKQMQV
Sbjct: 241 FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVILLGHSDSYVRDKQMQV 300

Query: 301 TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF 360
           TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF
Sbjct: 301 TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF 360

Query: 361 AKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGS 420
           AKEVTKRVDTWNG WKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVG+
Sbjct: 361 AKEVTKRVDTWNGEWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGT 420

Query: 421 ITSSAGALSCRRGYRC 437
           ITSSAGALSCRRGYRC
Sbjct: 421 ITSSAGALSCRRGYRC 434

BLAST of Lag0033175 vs. NCBI nr
Match: XP_023532769.1 (probable pectate lyase 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 857.8 bits (2215), Expect = 4.0e-245
Identity = 417/436 (95.64%), Postives = 426/436 (97.71%), Query Frame = 0

Query: 1   MAVSSLRVLRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSSSMADGYVKNEHAVDNPE 60
           MAV SLR L FS ASSLLFLLL++LFVHSSFAGDERLQSFNSSSMAD  VKNEHAVDNPE
Sbjct: 1   MAV-SLR-LHFSMASSLLFLLLHILFVHSSFAGDERLQSFNSSSMADWNVKNEHAVDNPE 60

Query: 61  EIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNAVG 120
           E+ASMVD  IRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLA+CGIGFGRNAVG
Sbjct: 61  EVASMVDSSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLANCGIGFGRNAVG 120

Query: 121 GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID 180
           GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID
Sbjct: 121 GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID 180

Query: 181 ARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISI 240
            RGANVHIAYGACITIQF+TNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDG+SI
Sbjct: 181 GRGANVHIAYGACITIQFVTNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGVSI 240

Query: 241 FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQV 300
           FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEV+LLGHSDSYVRDKQMQV
Sbjct: 241 FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVILLGHSDSYVRDKQMQV 300

Query: 301 TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF 360
           TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF
Sbjct: 301 TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF 360

Query: 361 AKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGS 420
           AKEVTKRVDTWNG WKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVG+
Sbjct: 361 AKEVTKRVDTWNGEWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGT 420

Query: 421 ITSSAGALSCRRGYRC 437
           ITS+AGALSCRRGYRC
Sbjct: 421 ITSTAGALSCRRGYRC 434

BLAST of Lag0033175 vs. NCBI nr
Match: XP_022931133.1 (probable pectate lyase 8 [Cucurbita moschata])

HSP 1 Score: 856.7 bits (2212), Expect = 8.9e-245
Identity = 414/432 (95.83%), Postives = 422/432 (97.69%), Query Frame = 0

Query: 5   SLRVLRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSSSMADGYVKNEHAVDNPEEIAS 64
           SLR L FS ASSLLFLLL++LFVHSSFAGDERLQ FNSSSMAD  VKNEHAVDNPEEIAS
Sbjct: 4   SLR-LHFSMASSLLFLLLHILFVHSSFAGDERLQRFNSSSMADWNVKNEHAVDNPEEIAS 63

Query: 65  MVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNAVGGRDG 124
           +VD  IRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLA+CGIGFGRNAVGGRDG
Sbjct: 64  LVDSSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLANCGIGFGRNAVGGRDG 123

Query: 125 RYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDARGA 184
           RYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID RGA
Sbjct: 124 RYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGA 183

Query: 185 NVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSS 244
           NVHIAYGACITIQF+TNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDG+SIFGSS
Sbjct: 184 NVHIAYGACITIQFVTNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGVSIFGSS 243

Query: 245 HIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAY 304
           HIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEV+LLGHSDSYVRDKQMQVTIAY
Sbjct: 244 HIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVILLGHSDSYVRDKQMQVTIAY 303

Query: 305 NHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEV 364
           NHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEV
Sbjct: 304 NHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEV 363

Query: 365 TKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGSITSS 424
           TKRVDTWNG WKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVG+ITSS
Sbjct: 364 TKRVDTWNGEWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGTITSS 423

Query: 425 AGALSCRRGYRC 437
           AGALSCRRGYRC
Sbjct: 424 AGALSCRRGYRC 434

BLAST of Lag0033175 vs. NCBI nr
Match: XP_038887964.1 (probable pectate lyase 8 [Benincasa hispida])

HSP 1 Score: 849.7 bits (2194), Expect = 1.1e-242
Identity = 409/426 (96.01%), Postives = 420/426 (98.59%), Query Frame = 0

Query: 12  SSASSLLFLLLNVL-FVHSSFAGDERLQSFNSSSMADGYVKNEHAVDNPEEIASMVDLRI 71
           S+ASS+LFLLL ++ FV SSFAGDERLQSFNSSSMAD Y+KNEHAVDNPEEIASMVD RI
Sbjct: 4   STASSILFLLLLIIFFVQSSFAGDERLQSFNSSSMADQYIKNEHAVDNPEEIASMVDSRI 63

Query: 72  RNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNAVGGRDGRYYVVS 131
           RNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLA+CGIGFGRNAVGGRDGRYYVVS
Sbjct: 64  RNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLANCGIGFGRNAVGGRDGRYYVVS 123

Query: 132 DPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDARGANVHIAY 191
           DPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID RGANVHIAY
Sbjct: 124 DPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGANVHIAY 183

Query: 192 GACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDH 251
           GACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDH
Sbjct: 184 GACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDH 243

Query: 252 NSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEG 311
           NSLS+CADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEG
Sbjct: 244 NSLSTCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEG 303

Query: 312 LIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEVTKRVDT 371
           LIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEVTKRVDT
Sbjct: 304 LIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEVTKRVDT 363

Query: 372 WNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGSITSSAGALSC 431
           +NGVWKHWNWRSEGDL+LNGAYFTPSGAGAAASYARASSLGAKSSSLVGSITS+AGALSC
Sbjct: 364 YNGVWKHWNWRSEGDLMLNGAYFTPSGAGAAASYARASSLGAKSSSLVGSITSNAGALSC 423

Query: 432 RRGYRC 437
           RRG+RC
Sbjct: 424 RRGFRC 429

BLAST of Lag0033175 vs. ExPASy Swiss-Prot
Match: Q9M8Z8 (Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 5.3e-200
Identity = 322/385 (83.64%), Postives = 354/385 (91.95%), Query Frame = 0

Query: 52  NEHAVDNPEEIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCG 111
           +EHAV+NP+E+A+MVD+ IRNSTERR LG+FSC TGNPIDDCWRCD +WQLRRK LADC 
Sbjct: 32  HEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKRLADCS 91

Query: 112 IGFGRNAVGGRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQEL 171
           IGFGRNA+GGRDGR+YVV+DPGDDDPVNP PGTLRHAVIQD PLWI+FKRDMVITLKQEL
Sbjct: 92  IGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQEL 151

Query: 172 IMNSFKTIDARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRT 231
           IMNSFKTID RG NVHIA GAC+TIQ++TN+I+HG+H+HDCKPTGNAMVRSSPSHYG+R+
Sbjct: 152 IMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSHYGFRS 211

Query: 232 MADGDGISIFGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDS 291
           MADGD ISIFGSSHIWIDHNSLS+CADGL+DAVM STAIT+SNN+FTHHNEVMLLGHSDS
Sbjct: 212 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNEVMLLGHSDS 271

Query: 292 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGN 351
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA PTINSQGN
Sbjct: 272 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAGPTINSQGN 331

Query: 352 RYLAPANPFAKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLG 411
           R+LAP NPFAKEVTKR  T    WKHWNWRSEGDL LNGA+FT SGAGA A+YARASSL 
Sbjct: 332 RFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGANYARASSLS 391

Query: 412 AKSSSLVGSITSSAGALSCRRGYRC 437
           AKSSSLVG++TS +GAL+CR G RC
Sbjct: 392 AKSSSLVGTMTSYSGALNCRAGRRC 416

BLAST of Lag0033175 vs. ExPASy Swiss-Prot
Match: Q93WF1 (Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 1.2e-199
Identity = 327/385 (84.94%), Postives = 350/385 (90.91%), Query Frame = 0

Query: 52  NEHAVDNPEEIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCG 111
           +EHAV+NPEE+A+MVD+ IRNST RR LG+FSC TGNPIDDCWRCD RWQ RRKHLA+C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 112 IGFGRNAVGGRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQEL 171
           IGFGRNA+GGRDGRYYVVSDP DD+PVNP+PGTLRHAVIQ+ PLWIVFKRDMVITLK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 172 IMNSFKTIDARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRT 231
           IMNSFKTID RG NVHIA GACITIQF+TN+IIHG+HIHDC+PTGNAMVRSSPSHYGWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 232 MADGDGISIFGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDS 291
           MADGDGISIFGSSHIWIDHNSLS+CADGLIDAVM STAITISNNYFTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 292 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGN 351
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 352 RYLAPANPFAKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLG 411
           RYLAP N FAKEVTKR       W+HWNWRSEGDL LNGA+FT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 412 AKSSSLVGSITSSAGALSCRRGYRC 437
           AKSSSLVG IT +AGAL+CR G RC
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417

BLAST of Lag0033175 vs. ExPASy Swiss-Prot
Match: Q9LJ42 (Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2)

HSP 1 Score: 691.0 bits (1782), Expect = 8.5e-198
Identity = 337/444 (75.90%), Postives = 373/444 (84.01%), Query Frame = 0

Query: 1   MAVSSLRVLRFSSASSLLFLLLNVLFVHSSFAGD-ERLQSFNSSSMA-------DGYVKN 60
           M + S   L  S+   +L L +N     S+ A + E L S +SS+ +       D    N
Sbjct: 1   MVIFSRSFLALSTTLIILALCIN----SSTMAQETEDLNSHSSSNSSTANKLPNDDGAWN 60

Query: 61  EHAVDNPEEIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGI 120
           EHAV NPEE+A+MVD++I+NSTERR LGFFSC TGNPIDDCWRCD  W LRRK LA+C I
Sbjct: 61  EHAVKNPEEVAAMVDMKIKNSTERRRLGFFSCATGNPIDDCWRCDRNWHLRRKRLANCAI 120

Query: 121 GFGRNAVGGRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELI 180
           GFGRNA+GGRDGRYYVV+DP D D VNPRPGTLRHAVIQDRPLWIVFKRDMVITL QELI
Sbjct: 121 GFGRNAIGGRDGRYYVVTDPSDHDAVNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQELI 180

Query: 181 MNSFKTIDARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTM 240
           MNSFKTID RG NV IA GACITIQ++TN+IIHG+++HDC+ TGNAMVRSSPSHYGWRTM
Sbjct: 181 MNSFKTIDGRGVNVAIAGGACITIQYVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTM 240

Query: 241 ADGDGISIFGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSY 300
           ADGD ISIFGSSHIWIDHNSLS+CADGLIDA+MGSTAITISNNY THHNEVML+GHSDSY
Sbjct: 241 ADGDAISIFGSSHIWIDHNSLSNCADGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSY 300

Query: 301 VRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNR 360
            RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSA+PTINSQGNR
Sbjct: 301 TRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNR 360

Query: 361 YLAPANPFAKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGA 420
           +LAP NPFAKEVTKRV +W G WK WNWRS+GDL+LNGAYFT SGA A ASYARASSLGA
Sbjct: 361 FLAPGNPFAKEVTKRVGSWQGEWKQWNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGA 420

Query: 421 KSSSLVGSITSSAGALSCRRGYRC 437
           K +S+V  +T S+GAL CR G RC
Sbjct: 421 KPASVVSMLTYSSGALKCRIGMRC 440

BLAST of Lag0033175 vs. ExPASy Swiss-Prot
Match: Q940Q1 (Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 683.3 bits (1762), Expect = 1.8e-195
Identity = 321/389 (82.52%), Postives = 347/389 (89.20%), Query Frame = 0

Query: 52  NEHAVDNPEEIA----SMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHL 111
           N+HAV NP+E+A    ++ ++ +RN TERR LG+F+CGTGNPIDDCWRCDP W   RK L
Sbjct: 41  NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDDCWRCDPNWHKNRKRL 100

Query: 112 ADCGIGFGRNAVGGRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITL 171
           ADCGIGFGRNA+GGRDGR+YVV+DP DD+PVNPRPGTLRHAVIQDRPLWIVFKRDMVI L
Sbjct: 101 ADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDMVIQL 160

Query: 172 KQELIMNSFKTIDARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHY 231
           KQELI+NSFKTID RGANVHIA G CITIQF+TNVI+HGLHIHDCKPTGNAMVRSS +H+
Sbjct: 161 KQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSSETHF 220

Query: 232 GWRTMADGDGISIFGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLG 291
           GWRTMADGD ISIFGSSH+WIDHNSLS CADGL+DAVMGSTAITISNN+ THHNEVMLLG
Sbjct: 221 GWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEVMLLG 280

Query: 292 HSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTIN 351
           HSDSY+RDK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA+PTIN
Sbjct: 281 HSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 340

Query: 352 SQGNRYLAPANPFAKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARA 411
           SQGNRY AP NPFAKEVTKRVDT    WK WNWRSEGDLL NGAYFT SGA A+ SYARA
Sbjct: 341 SQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGSYARA 400

Query: 412 SSLGAKSSSLVGSITSSAGALSCRRGYRC 437
           SSL AKSSSLVG ITS AGAL CRRG +C
Sbjct: 401 SSLSAKSSSLVGHITSDAGALPCRRGRQC 429

BLAST of Lag0033175 vs. ExPASy Swiss-Prot
Match: Q9SVQ6 (Putative pectate lyase 14 OS=Arabidopsis thaliana OX=3702 GN=At4g13210 PE=3 SV=2)

HSP 1 Score: 674.9 bits (1740), Expect = 6.3e-193
Identity = 331/438 (75.57%), Postives = 368/438 (84.02%), Query Frame = 0

Query: 7   RVLRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSS-------SMADGYVKNEHAVDNP 66
           R L   SA+ ++FL    LF+H +   + R     SS       +++DG   +EHAV +P
Sbjct: 5   RTLFSISATLIIFL---ALFLHVNAVQETREPKHESSRNTSTVDNLSDG-EWHEHAVKDP 64

Query: 67  EEIASMVDLRIRNSTERRNLGFF-SCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNA 126
           EEIA+MVD+ IRNST RR LGFF SC TGNPIDDCWRCD +W  RRK LADC IGFGRNA
Sbjct: 65  EEIAAMVDMSIRNSTYRRKLGFFSSCSTGNPIDDCWRCDKKWHRRRKRLADCAIGFGRNA 124

Query: 127 VGGRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKT 186
           VGGRDGRYY+V+DP D DPV P+PGTLR+AVIQD PLWIVFKRDMVITL QELIMNSFKT
Sbjct: 125 VGGRDGRYYIVTDPSDHDPVTPKPGTLRYAVIQDEPLWIVFKRDMVITLSQELIMNSFKT 184

Query: 187 IDARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGI 246
           ID RG NVHIA GAC+T+Q++TN+IIHG++IHDCK TGNAMVRSS SHYGWRTMADGDGI
Sbjct: 185 IDGRGVNVHIAGGACLTVQYVTNIIIHGINIHDCKRTGNAMVRSSESHYGWRTMADGDGI 244

Query: 247 SIFGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQM 306
           SIFGSSHIWIDHNSLSSCADGLIDA+MGSTAITISNNY THHNE +LLGH+DSY RDK M
Sbjct: 245 SIFGSSHIWIDHNSLSSCADGLIDAIMGSTAITISNNYLTHHNEAILLGHTDSYTRDKMM 304

Query: 307 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPAN 366
           QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA+PTINSQGNR+LAP N
Sbjct: 305 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPGN 364

Query: 367 PFAKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLV 426
            FAKEVTKRV    G W +WNWRS+GDL+LNGAYFT SGAGA+A+YARASSL AKSSSLV
Sbjct: 365 RFAKEVTKRVGAGKGEWNNWNWRSQGDLMLNGAYFTSSGAGASANYARASSLAAKSSSLV 424

Query: 427 GSITSSAGALSCRRGYRC 437
           G +TSS+GAL CR G  C
Sbjct: 425 GMLTSSSGALKCRIGTLC 438

BLAST of Lag0033175 vs. ExPASy TrEMBL
Match: A0A6J1K6I7 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111491608 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 6.6e-246
Identity = 419/436 (96.10%), Postives = 426/436 (97.71%), Query Frame = 0

Query: 1   MAVSSLRVLRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSSSMADGYVKNEHAVDNPE 60
           MAV SLR L FS ASSLLFLLL++LFVHSSFAGDERLQSFNSSSMAD  VKNEHAVDNPE
Sbjct: 1   MAV-SLR-LHFSMASSLLFLLLHILFVHSSFAGDERLQSFNSSSMADWNVKNEHAVDNPE 60

Query: 61  EIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNAVG 120
           EIASMVD  IRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLA+CGIGFGRNAVG
Sbjct: 61  EIASMVDSSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLANCGIGFGRNAVG 120

Query: 121 GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID 180
           GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID
Sbjct: 121 GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID 180

Query: 181 ARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISI 240
            RGANVHIAYGACITIQF+TNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDG+SI
Sbjct: 181 GRGANVHIAYGACITIQFVTNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGVSI 240

Query: 241 FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQV 300
           FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEV+LLGHSDSYVRDKQMQV
Sbjct: 241 FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVILLGHSDSYVRDKQMQV 300

Query: 301 TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF 360
           TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF
Sbjct: 301 TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF 360

Query: 361 AKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGS 420
           AKEVTKRVDTWNG WKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVG+
Sbjct: 361 AKEVTKRVDTWNGEWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGT 420

Query: 421 ITSSAGALSCRRGYRC 437
           ITSSAGALSCRRGYRC
Sbjct: 421 ITSSAGALSCRRGYRC 434

BLAST of Lag0033175 vs. ExPASy TrEMBL
Match: A0A6J1EXJ0 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111437403 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.3e-245
Identity = 414/432 (95.83%), Postives = 422/432 (97.69%), Query Frame = 0

Query: 5   SLRVLRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSSSMADGYVKNEHAVDNPEEIAS 64
           SLR L FS ASSLLFLLL++LFVHSSFAGDERLQ FNSSSMAD  VKNEHAVDNPEEIAS
Sbjct: 4   SLR-LHFSMASSLLFLLLHILFVHSSFAGDERLQRFNSSSMADWNVKNEHAVDNPEEIAS 63

Query: 65  MVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNAVGGRDG 124
           +VD  IRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLA+CGIGFGRNAVGGRDG
Sbjct: 64  LVDSSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLANCGIGFGRNAVGGRDG 123

Query: 125 RYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDARGA 184
           RYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID RGA
Sbjct: 124 RYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGA 183

Query: 185 NVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSS 244
           NVHIAYGACITIQF+TNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDG+SIFGSS
Sbjct: 184 NVHIAYGACITIQFVTNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGVSIFGSS 243

Query: 245 HIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAY 304
           HIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEV+LLGHSDSYVRDKQMQVTIAY
Sbjct: 244 HIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVILLGHSDSYVRDKQMQVTIAY 303

Query: 305 NHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEV 364
           NHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEV
Sbjct: 304 NHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEV 363

Query: 365 TKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGSITSS 424
           TKRVDTWNG WKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVG+ITSS
Sbjct: 364 TKRVDTWNGEWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGTITSS 423

Query: 425 AGALSCRRGYRC 437
           AGALSCRRGYRC
Sbjct: 424 AGALSCRRGYRC 434

BLAST of Lag0033175 vs. ExPASy TrEMBL
Match: A0A6J1HRI0 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111466837 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 7.1e-240
Identity = 403/428 (94.16%), Postives = 414/428 (96.73%), Query Frame = 0

Query: 9   LRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSSSMADGYVKNEHAVDNPEEIASMVDL 68
           L+FS+AS + FLLLN+LFV SSFAGD+RLQSFNSSSMAD Y KNEHAVDNPEEIASMVDL
Sbjct: 7   LQFSTASLMFFLLLNILFVQSSFAGDQRLQSFNSSSMADRYSKNEHAVDNPEEIASMVDL 66

Query: 69  RIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNAVGGRDGRYYV 128
            IRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRK LADCGIGFGRNAVGGRDGRYYV
Sbjct: 67  SIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKRLADCGIGFGRNAVGGRDGRYYV 126

Query: 129 VSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDARGANVHI 188
           VSDP DDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID RG NVHI
Sbjct: 127 VSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHI 186

Query: 189 AYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWI 248
           AYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWI
Sbjct: 187 AYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWI 246

Query: 249 DHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFG 308
           DHNSLSSCADGLIDA+MGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFG
Sbjct: 247 DHNSLSSCADGLIDAIMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFG 306

Query: 309 EGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEVTKRV 368
           EGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSA+PTINSQGNRYLAP NPFAKEVTKRV
Sbjct: 307 EGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRV 366

Query: 369 DTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGSITSSAGAL 428
            T NGVW+HWNWRSEGDL+LNGAYFTPSGAGA ASYARASSLGAKSSSLVGS+TS+AGAL
Sbjct: 367 VTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNAGAL 426

Query: 429 SCRRGYRC 437
           SCRRGYRC
Sbjct: 427 SCRRGYRC 434

BLAST of Lag0033175 vs. ExPASy TrEMBL
Match: A0A6J1HFR5 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.2e-239
Identity = 402/428 (93.93%), Postives = 416/428 (97.20%), Query Frame = 0

Query: 9   LRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSSSMADGYVKNEHAVDNPEEIASMVDL 68
           L+FS+AS +LFLLLN+LFV SSFAGD+RLQSFNSSSMAD YVKNEHAVDNPEEIASMVDL
Sbjct: 7   LQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDL 66

Query: 69  RIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNAVGGRDGRYYV 128
            IRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRK LA+CGIGFGRNAVGGRDGRYYV
Sbjct: 67  SIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYV 126

Query: 129 VSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDARGANVHI 188
           VSDP DDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID RG NVHI
Sbjct: 127 VSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHI 186

Query: 189 AYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWI 248
           AYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWI
Sbjct: 187 AYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWI 246

Query: 249 DHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFG 308
           DHNSLSSC+DGLIDA+MGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFG
Sbjct: 247 DHNSLSSCSDGLIDAIMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFG 306

Query: 309 EGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPFAKEVTKRV 368
           EGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSA+PTINSQGNRYLAP NPFAKEVTKRV
Sbjct: 307 EGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRV 366

Query: 369 DTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGSITSSAGAL 428
            T NGVW+HWNWRSEGDL+LNGAYFTPSGAGA ASYARASSLGAKSSSLVGS+TS+AGAL
Sbjct: 367 VTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNAGAL 426

Query: 429 SCRRGYRC 437
           SCRRG+RC
Sbjct: 427 SCRRGFRC 434

BLAST of Lag0033175 vs. ExPASy TrEMBL
Match: A0A1S3BH56 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489803 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.1e-239
Identity = 406/436 (93.12%), Postives = 418/436 (95.87%), Query Frame = 0

Query: 1   MAVSSLRVLRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSSSMADGYVKNEHAVDNPE 60
           MAVS    +   +++SLL LLL + F+HSSFA DERLQSFNSSSM   Y+KNEHAVDNPE
Sbjct: 1   MAVS----VTLRTSTSLLLLLLLIFFLHSSFAEDERLQSFNSSSMPYRYIKNEHAVDNPE 60

Query: 61  EIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNAVG 120
           EIASMVD  IRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLA+CGIGFGRNAVG
Sbjct: 61  EIASMVDSSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLANCGIGFGRNAVG 120

Query: 121 GRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID 180
           GRDGRYYVVSDPGDDDP+NPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID
Sbjct: 121 GRDGRYYVVSDPGDDDPINPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTID 180

Query: 181 ARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISI 240
            RGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISI
Sbjct: 181 GRGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISI 240

Query: 241 FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQV 300
           FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQV
Sbjct: 241 FGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQV 300

Query: 301 TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF 360
           TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF
Sbjct: 301 TIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPANPF 360

Query: 361 AKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLVGS 420
           AKEVTKRVDT NGVWKHWNWRSEGDL+LNGAYFTPSGAGAAASYARASSLGAKSSSLVGS
Sbjct: 361 AKEVTKRVDTHNGVWKHWNWRSEGDLMLNGAYFTPSGAGAAASYARASSLGAKSSSLVGS 420

Query: 421 ITSSAGALSCRRGYRC 437
           ITS+AGAL+CRRGYRC
Sbjct: 421 ITSNAGALACRRGYRC 432

BLAST of Lag0033175 vs. TAIR 10
Match: AT3G07010.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 698.4 bits (1801), Expect = 3.8e-201
Identity = 322/385 (83.64%), Postives = 354/385 (91.95%), Query Frame = 0

Query: 52  NEHAVDNPEEIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCG 111
           +EHAV+NP+E+A+MVD+ IRNSTERR LG+FSC TGNPIDDCWRCD +WQLRRK LADC 
Sbjct: 32  HEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKRLADCS 91

Query: 112 IGFGRNAVGGRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQEL 171
           IGFGRNA+GGRDGR+YVV+DPGDDDPVNP PGTLRHAVIQD PLWI+FKRDMVITLKQEL
Sbjct: 92  IGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQEL 151

Query: 172 IMNSFKTIDARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRT 231
           IMNSFKTID RG NVHIA GAC+TIQ++TN+I+HG+H+HDCKPTGNAMVRSSPSHYG+R+
Sbjct: 152 IMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSHYGFRS 211

Query: 232 MADGDGISIFGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDS 291
           MADGD ISIFGSSHIWIDHNSLS+CADGL+DAVM STAIT+SNN+FTHHNEVMLLGHSDS
Sbjct: 212 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNEVMLLGHSDS 271

Query: 292 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGN 351
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA PTINSQGN
Sbjct: 272 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAGPTINSQGN 331

Query: 352 RYLAPANPFAKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLG 411
           R+LAP NPFAKEVTKR  T    WKHWNWRSEGDL LNGA+FT SGAGA A+YARASSL 
Sbjct: 332 RFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGANYARASSLS 391

Query: 412 AKSSSLVGSITSSAGALSCRRGYRC 437
           AKSSSLVG++TS +GAL+CR G RC
Sbjct: 392 AKSSSLVGTMTSYSGALNCRAGRRC 416

BLAST of Lag0033175 vs. TAIR 10
Match: AT5G48900.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 697.2 bits (1798), Expect = 8.4e-201
Identity = 327/385 (84.94%), Postives = 350/385 (90.91%), Query Frame = 0

Query: 52  NEHAVDNPEEIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCG 111
           +EHAV+NPEE+A+MVD+ IRNST RR LG+FSC TGNPIDDCWRCD RWQ RRKHLA+C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 112 IGFGRNAVGGRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQEL 171
           IGFGRNA+GGRDGRYYVVSDP DD+PVNP+PGTLRHAVIQ+ PLWIVFKRDMVITLK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 172 IMNSFKTIDARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRT 231
           IMNSFKTID RG NVHIA GACITIQF+TN+IIHG+HIHDC+PTGNAMVRSSPSHYGWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 232 MADGDGISIFGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDS 291
           MADGDGISIFGSSHIWIDHNSLS+CADGLIDAVM STAITISNNYFTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 292 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGN 351
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 352 RYLAPANPFAKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLG 411
           RYLAP N FAKEVTKR       W+HWNWRSEGDL LNGA+FT SG+G  ASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 412 AKSSSLVGSITSSAGALSCRRGYRC 437
           AKSSSLVG IT +AGAL+CR G RC
Sbjct: 393 AKSSSLVGVITYNAGALNCRGGRRC 417

BLAST of Lag0033175 vs. TAIR 10
Match: AT3G24670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 691.0 bits (1782), Expect = 6.0e-199
Identity = 337/444 (75.90%), Postives = 373/444 (84.01%), Query Frame = 0

Query: 1   MAVSSLRVLRFSSASSLLFLLLNVLFVHSSFAGD-ERLQSFNSSSMA-------DGYVKN 60
           M + S   L  S+   +L L +N     S+ A + E L S +SS+ +       D    N
Sbjct: 1   MVIFSRSFLALSTTLIILALCIN----SSTMAQETEDLNSHSSSNSSTANKLPNDDGAWN 60

Query: 61  EHAVDNPEEIASMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHLADCGI 120
           EHAV NPEE+A+MVD++I+NSTERR LGFFSC TGNPIDDCWRCD  W LRRK LA+C I
Sbjct: 61  EHAVKNPEEVAAMVDMKIKNSTERRRLGFFSCATGNPIDDCWRCDRNWHLRRKRLANCAI 120

Query: 121 GFGRNAVGGRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELI 180
           GFGRNA+GGRDGRYYVV+DP D D VNPRPGTLRHAVIQDRPLWIVFKRDMVITL QELI
Sbjct: 121 GFGRNAIGGRDGRYYVVTDPSDHDAVNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQELI 180

Query: 181 MNSFKTIDARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTM 240
           MNSFKTID RG NV IA GACITIQ++TN+IIHG+++HDC+ TGNAMVRSSPSHYGWRTM
Sbjct: 181 MNSFKTIDGRGVNVAIAGGACITIQYVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTM 240

Query: 241 ADGDGISIFGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSY 300
           ADGD ISIFGSSHIWIDHNSLS+CADGLIDA+MGSTAITISNNY THHNEVML+GHSDSY
Sbjct: 241 ADGDAISIFGSSHIWIDHNSLSNCADGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSY 300

Query: 301 VRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNR 360
            RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSA+PTINSQGNR
Sbjct: 301 TRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNR 360

Query: 361 YLAPANPFAKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGA 420
           +LAP NPFAKEVTKRV +W G WK WNWRS+GDL+LNGAYFT SGA A ASYARASSLGA
Sbjct: 361 FLAPGNPFAKEVTKRVGSWQGEWKQWNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGA 420

Query: 421 KSSSLVGSITSSAGALSCRRGYRC 437
           K +S+V  +T S+GAL CR G RC
Sbjct: 421 KPASVVSMLTYSSGALKCRIGMRC 440

BLAST of Lag0033175 vs. TAIR 10
Match: AT1G04680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 683.3 bits (1762), Expect = 1.3e-196
Identity = 321/389 (82.52%), Postives = 347/389 (89.20%), Query Frame = 0

Query: 52  NEHAVDNPEEIA----SMVDLRIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKHL 111
           N+HAV NP+E+A    ++ ++ +RN TERR LG+F+CGTGNPIDDCWRCDP W   RK L
Sbjct: 41  NQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDDCWRCDPNWHKNRKRL 100

Query: 112 ADCGIGFGRNAVGGRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITL 171
           ADCGIGFGRNA+GGRDGR+YVV+DP DD+PVNPRPGTLRHAVIQDRPLWIVFKRDMVI L
Sbjct: 101 ADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDMVIQL 160

Query: 172 KQELIMNSFKTIDARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHY 231
           KQELI+NSFKTID RGANVHIA G CITIQF+TNVI+HGLHIHDCKPTGNAMVRSS +H+
Sbjct: 161 KQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSSETHF 220

Query: 232 GWRTMADGDGISIFGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLG 291
           GWRTMADGD ISIFGSSH+WIDHNSLS CADGL+DAVMGSTAITISNN+ THHNEVMLLG
Sbjct: 221 GWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEVMLLG 280

Query: 292 HSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTIN 351
           HSDSY+RDK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA+PTIN
Sbjct: 281 HSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTIN 340

Query: 352 SQGNRYLAPANPFAKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARA 411
           SQGNRY AP NPFAKEVTKRVDT    WK WNWRSEGDLL NGAYFT SGA A+ SYARA
Sbjct: 341 SQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGSYARA 400

Query: 412 SSLGAKSSSLVGSITSSAGALSCRRGYRC 437
           SSL AKSSSLVG ITS AGAL CRRG +C
Sbjct: 401 SSLSAKSSSLVGHITSDAGALPCRRGRQC 429

BLAST of Lag0033175 vs. TAIR 10
Match: AT4G13210.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 674.9 bits (1740), Expect = 4.5e-194
Identity = 331/438 (75.57%), Postives = 368/438 (84.02%), Query Frame = 0

Query: 7   RVLRFSSASSLLFLLLNVLFVHSSFAGDERLQSFNSS-------SMADGYVKNEHAVDNP 66
           R L   SA+ ++FL    LF+H +   + R     SS       +++DG   +EHAV +P
Sbjct: 5   RTLFSISATLIIFL---ALFLHVNAVQETREPKHESSRNTSTVDNLSDG-EWHEHAVKDP 64

Query: 67  EEIASMVDLRIRNSTERRNLGFF-SCGTGNPIDDCWRCDPRWQLRRKHLADCGIGFGRNA 126
           EEIA+MVD+ IRNST RR LGFF SC TGNPIDDCWRCD +W  RRK LADC IGFGRNA
Sbjct: 65  EEIAAMVDMSIRNSTYRRKLGFFSSCSTGNPIDDCWRCDKKWHRRRKRLADCAIGFGRNA 124

Query: 127 VGGRDGRYYVVSDPGDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKT 186
           VGGRDGRYY+V+DP D DPV P+PGTLR+AVIQD PLWIVFKRDMVITL QELIMNSFKT
Sbjct: 125 VGGRDGRYYIVTDPSDHDPVTPKPGTLRYAVIQDEPLWIVFKRDMVITLSQELIMNSFKT 184

Query: 187 IDARGANVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGI 246
           ID RG NVHIA GAC+T+Q++TN+IIHG++IHDCK TGNAMVRSS SHYGWRTMADGDGI
Sbjct: 185 IDGRGVNVHIAGGACLTVQYVTNIIIHGINIHDCKRTGNAMVRSSESHYGWRTMADGDGI 244

Query: 247 SIFGSSHIWIDHNSLSSCADGLIDAVMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQM 306
           SIFGSSHIWIDHNSLSSCADGLIDA+MGSTAITISNNY THHNE +LLGH+DSY RDK M
Sbjct: 245 SIFGSSHIWIDHNSLSSCADGLIDAIMGSTAITISNNYLTHHNEAILLGHTDSYTRDKMM 304

Query: 307 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSADPTINSQGNRYLAPAN 366
           QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA+PTINSQGNR+LAP N
Sbjct: 305 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPGN 364

Query: 367 PFAKEVTKRVDTWNGVWKHWNWRSEGDLLLNGAYFTPSGAGAAASYARASSLGAKSSSLV 426
            FAKEVTKRV    G W +WNWRS+GDL+LNGAYFT SGAGA+A+YARASSL AKSSSLV
Sbjct: 365 RFAKEVTKRVGAGKGEWNNWNWRSQGDLMLNGAYFTSSGAGASANYARASSLAAKSSSLV 424

Query: 427 GSITSSAGALSCRRGYRC 437
           G +TSS+GAL CR G  C
Sbjct: 425 GMLTSSSGALKCRIGTLC 438

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6606330.18.0e-24696.10putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022996350.11.4e-24596.10probable pectate lyase 8 [Cucurbita maxima][more]
XP_023532769.14.0e-24595.64probable pectate lyase 8 [Cucurbita pepo subsp. pepo][more]
XP_022931133.18.9e-24595.83probable pectate lyase 8 [Cucurbita moschata][more]
XP_038887964.11.1e-24296.01probable pectate lyase 8 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9M8Z85.3e-20083.64Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1[more]
Q93WF11.2e-19984.94Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1[more]
Q9LJ428.5e-19875.90Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2[more]
Q940Q11.8e-19582.52Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2[more]
Q9SVQ66.3e-19375.57Putative pectate lyase 14 OS=Arabidopsis thaliana OX=3702 GN=At4g13210 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1K6I76.6e-24696.10Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111491608 PE=3 SV=1[more]
A0A6J1EXJ04.3e-24595.83Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111437403 PE=3 SV=1[more]
A0A6J1HRI07.1e-24094.16Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111466837 PE=3 SV=1[more]
A0A6J1HFR51.2e-23993.93Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1[more]
A0A1S3BH562.1e-23993.12Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489803 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G07010.13.8e-20183.64Pectin lyase-like superfamily protein [more]
AT5G48900.18.4e-20184.94Pectin lyase-like superfamily protein [more]
AT3G24670.16.0e-19975.90Pectin lyase-like superfamily protein [more]
AT1G04680.11.3e-19682.52Pectin lyase-like superfamily protein [more]
AT4G13210.24.5e-19475.57Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 134..159
score: 69.96
coord: 314..333
score: 78.1
coord: 234..255
score: 68.61
coord: 336..355
score: 74.76
coord: 378..402
score: 55.24
coord: 110..127
score: 53.7
coord: 407..430
score: 40.48
coord: 170..186
score: 68.07
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 162..359
e-value: 2.0E-100
score: 349.6
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 182..351
e-value: 9.3E-19
score: 67.8
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 87..429
e-value: 1.2E-114
score: 384.9
NoneNo IPR availablePANTHERPTHR31683:SF81PECTATE LYASEcoord: 41..433
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 41..433
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 65..431

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033175.1Lag0033175.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity