Lag0033147 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0033147 vs. NCBI nr
Match: XP_038889041.1 (homeobox protein 9 [Benincasa hispida])

HSP 1 Score: 587.0 bits (1512), Expect = 1.2e-163
Identity = 319/389 (82.01%), Postives = 350/389 (89.97%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPAAARAAEIRGRTLLDIIRDEEPGVAKGLFGKDK 60
           MSIVEDGRSLAGYTLDDVLANNRLGPPA ARAAEIRGRTLLDIIRDEEP   KGLFGKDK
Sbjct: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPATARAAEIRGRTLLDIIRDEEPSAGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAGDYSN 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSD+PVH KRSLIG+R QVRF+T SSRN+ D   
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHTKRSLIGRRTQVRFNTHSSRNSTDNQT 120

Query: 121 RNQSDESSKHFRRQISRQT-TLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSRHNS 180
           RN S+ESSK  RRQ+SRQT T+NSSSDSSG HYDES+DYSS+PD+AP RIMRPQMSRHNS
Sbjct: 121 RNPSEESSKSTRRQMSRQTSTMNSSSDSSGSHYDESSDYSSSPDAAPTRIMRPQMSRHNS 180

Query: 181 IRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAAAA- 240
           +R+SNPME+YT+PTEPDRDNR+R+  S+ S++RM+SAREAVAAQEAADAASAAAAAAAA 
Sbjct: 181 VRVSNPMESYTEPTEPDRDNRIRY--SNFSDHRMMSAREAVAAQEAADAASAAAAAAAAA 240

Query: 241 -AAEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHETD 300
            AAEE+KEQ+EN      NEN NENENEESTTGSE EE  + P++EPVRMSLMDLLHETD
Sbjct: 241 VAAEEEKEQNEN------NENGNENENEESTTGSENEE--SSPTTEPVRMSLMDLLHETD 300

Query: 301 REMGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGGEFACCVCMVKHKNAPMAPCGHTF 360
           REMGFE +SYGMGF EDFFDDDEDD+YEEDEDDGTGGEF CCVCMVKHKN P+A CGHTF
Sbjct: 301 REMGFERSSYGMGFEEDFFDDDEDDEYEEDEDDGTGGEFTCCVCMVKHKNPPLAACGHTF 360

Query: 361 CRLCSKELMVSRGNCPTCNDFILEILDAF 387
           CRLCSKE+MVSRGNCPTC++FI EILDAF
Sbjct: 361 CRLCSKEMMVSRGNCPTCSNFISEILDAF 379

BLAST of Lag0033147 vs. NCBI nr
Match: KAA0053347.1 (E3 ubiquitin-protein ligase cblA-like [Cucumis melo var. makuwa] >TYK29191.1 E3 ubiquitin-protein ligase cblA-like [Cucumis melo var. makuwa])

HSP 1 Score: 583.2 bits (1502), Expect = 1.7e-162
Identity = 315/387 (81.40%), Postives = 352/387 (90.96%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPAAARAAEIRGRTLLDIIRDEEPGVAKGLFGKDK 60
           MS+VEDGRSLAGYTLDDVLANNRLGPPA ARA EIRGRTLLDIIRDEEP   KGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSAGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAGDYSN 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSD+PV  KRSLIG+RNQ+RF+T SS+N+ D  +
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVQTKRSLIGRRNQIRFNTGSSKNSTDNHS 120

Query: 121 RNQSDESSKHFRRQISRQT-TLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSRHNS 180
           R+ S+ESSK  RRQ+SRQT T+NSSSDSS FHYDES++YSS+PD+APVRIMRPQMSRHNS
Sbjct: 121 RHPSEESSKSTRRQMSRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 IRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAAAAA 240
           +R+SNPMENYTDPTEPDRDNR+RH  S+LS++RM+SAREAVAAQEAADAASAAAAAAAAA
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRH--SNLSDHRMMSAREAVAAQEAADAASAAAAAAAAA 240

Query: 241 AEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHETDRE 300
           AE  +E  E  +NEN N N NENE EESTTGSE EE ++ P+SEPVRMSLMDLLHETDRE
Sbjct: 241 AEAAEE--EKKQNENNNLNGNENEKEESTTGSEGEEESS-PASEPVRMSLMDLLHETDRE 300

Query: 301 MGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGGEFACCVCMVKHKNAPMAPCGHTFCR 360
           MGFE +SYGMGF E+FF+DDEDD+YEEDED+G GGEF+CCVCMVKHKNAP+A CGHTFCR
Sbjct: 301 MGFERSSYGMGFEENFFEDDEDDEYEEDEDNGNGGEFSCCVCMVKHKNAPLADCGHTFCR 360

Query: 361 LCSKELMVSRGNCPTCNDFILEILDAF 387
           LCSKELMVSRGNCPTC++FILEILDAF
Sbjct: 361 LCSKELMVSRGNCPTCSNFILEILDAF 382

BLAST of Lag0033147 vs. NCBI nr
Match: XP_008465469.2 (PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase cblA-like [Cucumis melo])

HSP 1 Score: 578.6 bits (1490), Expect = 4.1e-161
Identity = 311/387 (80.36%), Postives = 350/387 (90.44%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPAAARAAEIRGRTLLDIIRDEEPGVAKGLFGKDK 60
           MS+VEDGRSLAGYTLDDVLANNRLGPPA ARA EIRGRTLLDIIRDEEP   KGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSAGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAGDYSN 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSD+PV  KRSLIG+RNQ+RF+T SS+N+ D  +
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVQTKRSLIGRRNQIRFNTGSSKNSTDNHS 120

Query: 121 RNQSDESSKHFRRQISRQT-TLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSRHNS 180
           R+ S+ESSK  RRQ+SRQT T+NSSSDSS FHYDES++YSS+PD+APVRIMRPQMSRHNS
Sbjct: 121 RHPSEESSKSTRRQMSRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 IRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAAAAA 240
           +R+SNPMENYTDPTEPDRDNR+RH  S+LS++RM+SAREAVAAQEAADAASAAAAAAAAA
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRH--SNLSDHRMMSAREAVAAQEAADAASAAAAAAAAA 240

Query: 241 AEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHETDRE 300
              ++ +    +NEN N N NENE EESTTGSE EE ++ P+SEPVRMSLMDLLHETDRE
Sbjct: 241 GSSRRRK---KQNENNNLNGNENEKEESTTGSEGEEESS-PASEPVRMSLMDLLHETDRE 300

Query: 301 MGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGGEFACCVCMVKHKNAPMAPCGHTFCR 360
           MGFE +SYGMGF E+FF+DDEDD+YEEDED+G GGEF+CCVCMVKHKNAP+A CGHTFCR
Sbjct: 301 MGFERSSYGMGFEENFFEDDEDDEYEEDEDNGNGGEFSCCVCMVKHKNAPLADCGHTFCR 360

Query: 361 LCSKELMVSRGNCPTCNDFILEILDAF 387
           LCSKELMVSRGNCPTC++FILEILDAF
Sbjct: 361 LCSKELMVSRGNCPTCSNFILEILDAF 381

BLAST of Lag0033147 vs. NCBI nr
Match: XP_011651651.1 (protein FAM9A [Cucumis sativus] >KGN58363.1 hypothetical protein Csa_000963 [Cucumis sativus])

HSP 1 Score: 576.6 bits (1485), Expect = 1.6e-160
Identity = 311/389 (79.95%), Postives = 349/389 (89.72%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPAAARAAEIRGRTLLDIIRDEEPGVAKGLFGKDK 60
           MS+VEDGRSLAGYTLDDVLANNRLGPPA ARA EIRGRTLLDIIRDEEP   KGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAGDYSN 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSD+PVHNKRSL+G+RNQVRF+T SS+N+ D  +
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120

Query: 121 RNQSDESSKHFRRQISRQT-TLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSRHNS 180
           R+ S+E+SK  RRQ+ RQT T+NSSSDSS FHYDES++YSS+PD+APVRIMRPQMSRHNS
Sbjct: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 IRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAAAA- 240
           +R+SNPMENYTDPTEPDRDNR+RH+  +LS +RM+SAREAVAAQEAADAASAAAAAAAA 
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRHL--NLSEHRMMSAREAVAAQEAADAASAAAAAAAAA 240

Query: 241 -AAEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHETD 300
            AAEE+K+Q     NEN NE+ NENE EESTT SEEEE  + P+SEPVRMSLMDLLHETD
Sbjct: 241 EAAEEEKKQ-----NENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETD 300

Query: 301 REMGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGGEFACCVCMVKHKNAPMAPCGHTF 360
           REMGFE +SYGMGF  +F ++DEDD+YEEDEDDG G EF+CCVCMVKHKN P+A CGHTF
Sbjct: 301 REMGFERSSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTF 360

Query: 361 CRLCSKELMVSRGNCPTCNDFILEILDAF 387
           CRLCSKELMVSRGNCPTC++FILEILDAF
Sbjct: 361 CRLCSKELMVSRGNCPTCSNFILEILDAF 382

BLAST of Lag0033147 vs. NCBI nr
Match: XP_022995965.1 (E3 ubiquitin-protein ligase rnf8-A-like [Cucurbita maxima])

HSP 1 Score: 559.7 bits (1441), Expect = 2.0e-155
Identity = 310/389 (79.69%), Postives = 344/389 (88.43%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPAA--ARAAEIRGRTLLDIIRDEEPGVAKGLFGK 60
           MS++E+GRSLAGYTLDDVLANNR GPPA+  ARAAEIRGRTLLD+IRDEEP  AKGLFGK
Sbjct: 1   MSLLEEGRSLAGYTLDDVLANNRFGPPASATARAAEIRGRTLLDVIRDEEPSAAKGLFGK 60

Query: 61  DKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAGDY 120
           DKRTWKSFR+KLRLKRAGSAWVSTVPIPTSDMP   KRSL+GKR+QVRF+TPSSRN+ D+
Sbjct: 61  DKRTWKSFREKLRLKRAGSAWVSTVPIPTSDMPFQPKRSLMGKRSQVRFNTPSSRNSTDH 120

Query: 121 SNRNQSDESSKHFRRQISRQ-TTLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSRH 180
           S RN S+ESSK FRRQ+SRQ +T+NSSSDS+ F YDES DYSS PDSAP R MRPQMSRH
Sbjct: 121 SARNPSEESSKSFRRQVSRQSSTMNSSSDSTPFQYDESFDYSSVPDSAPSR-MRPQMSRH 180

Query: 181 NSIRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAAA 240
           NSIRI NP ENYTDPTE DRDNRVRH+SS+ S  RM+SARE VAAQEAADA  AAAAAAA
Sbjct: 181 NSIRIPNPTENYTDPTESDRDNRVRHISSAFSERRMMSARETVAAQEAADA--AAAAAAA 240

Query: 241 AAAEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHETD 300
            AAEE+KE+++  E   + ENE+ENEN ESTTGSEE E  + P+SEPVRMSLMDLLHETD
Sbjct: 241 EAAEEEKEKEKEKE---QIENEDENENVESTTGSEEWEKVS-PTSEPVRMSLMDLLHETD 300

Query: 301 REMGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGGEFACCVCMVKHKNAPMAPCGHTF 360
           REMGFEG++YGMGF ED F D+ED  YEEDEDDG+GGEF+CCVCMVKHKNAPMAPCGHTF
Sbjct: 301 REMGFEGSNYGMGFEEDGFSDEEDVAYEEDEDDGSGGEFSCCVCMVKHKNAPMAPCGHTF 360

Query: 361 CRLCSKELMVSRGNCPTCNDFILEILDAF 387
           CRLCSKELMVSRGNCPTCNDFILE+L+AF
Sbjct: 361 CRLCSKELMVSRGNCPTCNDFILEMLEAF 382

BLAST of Lag0033147 vs. ExPASy TrEMBL
Match: A0A5D3E0G9 (E3 ubiquitin-protein ligase cblA-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold880G00010 PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 8.1e-163
Identity = 315/387 (81.40%), Postives = 352/387 (90.96%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPAAARAAEIRGRTLLDIIRDEEPGVAKGLFGKDK 60
           MS+VEDGRSLAGYTLDDVLANNRLGPPA ARA EIRGRTLLDIIRDEEP   KGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSAGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAGDYSN 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSD+PV  KRSLIG+RNQ+RF+T SS+N+ D  +
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVQTKRSLIGRRNQIRFNTGSSKNSTDNHS 120

Query: 121 RNQSDESSKHFRRQISRQT-TLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSRHNS 180
           R+ S+ESSK  RRQ+SRQT T+NSSSDSS FHYDES++YSS+PD+APVRIMRPQMSRHNS
Sbjct: 121 RHPSEESSKSTRRQMSRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 IRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAAAAA 240
           +R+SNPMENYTDPTEPDRDNR+RH  S+LS++RM+SAREAVAAQEAADAASAAAAAAAAA
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRH--SNLSDHRMMSAREAVAAQEAADAASAAAAAAAAA 240

Query: 241 AEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHETDRE 300
           AE  +E  E  +NEN N N NENE EESTTGSE EE ++ P+SEPVRMSLMDLLHETDRE
Sbjct: 241 AEAAEE--EKKQNENNNLNGNENEKEESTTGSEGEEESS-PASEPVRMSLMDLLHETDRE 300

Query: 301 MGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGGEFACCVCMVKHKNAPMAPCGHTFCR 360
           MGFE +SYGMGF E+FF+DDEDD+YEEDED+G GGEF+CCVCMVKHKNAP+A CGHTFCR
Sbjct: 301 MGFERSSYGMGFEENFFEDDEDDEYEEDEDNGNGGEFSCCVCMVKHKNAPLADCGHTFCR 360

Query: 361 LCSKELMVSRGNCPTCNDFILEILDAF 387
           LCSKELMVSRGNCPTC++FILEILDAF
Sbjct: 361 LCSKELMVSRGNCPTCSNFILEILDAF 382

BLAST of Lag0033147 vs. ExPASy TrEMBL
Match: A0A1S3CNY7 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase cblA-like OS=Cucumis melo OX=3656 GN=LOC103503074 PE=4 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 2.0e-161
Identity = 311/387 (80.36%), Postives = 350/387 (90.44%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPAAARAAEIRGRTLLDIIRDEEPGVAKGLFGKDK 60
           MS+VEDGRSLAGYTLDDVLANNRLGPPA ARA EIRGRTLLDIIRDEEP   KGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSAGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAGDYSN 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSD+PV  KRSLIG+RNQ+RF+T SS+N+ D  +
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVQTKRSLIGRRNQIRFNTGSSKNSTDNHS 120

Query: 121 RNQSDESSKHFRRQISRQT-TLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSRHNS 180
           R+ S+ESSK  RRQ+SRQT T+NSSSDSS FHYDES++YSS+PD+APVRIMRPQMSRHNS
Sbjct: 121 RHPSEESSKSTRRQMSRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 IRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAAAAA 240
           +R+SNPMENYTDPTEPDRDNR+RH  S+LS++RM+SAREAVAAQEAADAASAAAAAAAAA
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRH--SNLSDHRMMSAREAVAAQEAADAASAAAAAAAAA 240

Query: 241 AEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHETDRE 300
              ++ +    +NEN N N NENE EESTTGSE EE ++ P+SEPVRMSLMDLLHETDRE
Sbjct: 241 GSSRRRK---KQNENNNLNGNENEKEESTTGSEGEEESS-PASEPVRMSLMDLLHETDRE 300

Query: 301 MGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGGEFACCVCMVKHKNAPMAPCGHTFCR 360
           MGFE +SYGMGF E+FF+DDEDD+YEEDED+G GGEF+CCVCMVKHKNAP+A CGHTFCR
Sbjct: 301 MGFERSSYGMGFEENFFEDDEDDEYEEDEDNGNGGEFSCCVCMVKHKNAPLADCGHTFCR 360

Query: 361 LCSKELMVSRGNCPTCNDFILEILDAF 387
           LCSKELMVSRGNCPTC++FILEILDAF
Sbjct: 361 LCSKELMVSRGNCPTCSNFILEILDAF 381

BLAST of Lag0033147 vs. ExPASy TrEMBL
Match: A0A0A0L944 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G628230 PE=4 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 7.6e-161
Identity = 311/389 (79.95%), Postives = 349/389 (89.72%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPAAARAAEIRGRTLLDIIRDEEPGVAKGLFGKDK 60
           MS+VEDGRSLAGYTLDDVLANNRLGPPA ARA EIRGRTLLDIIRDEEP   KGLFGKDK
Sbjct: 1   MSMVEDGRSLAGYTLDDVLANNRLGPPATARATEIRGRTLLDIIRDEEPSTGKGLFGKDK 60

Query: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAGDYSN 120
           RTWKSFRDKLRLKRAGSAWVSTVPIPTSD+PVHNKRSL+G+RNQVRF+T SS+N+ D  +
Sbjct: 61  RTWKSFRDKLRLKRAGSAWVSTVPIPTSDIPVHNKRSLMGRRNQVRFNTGSSKNSPDNQS 120

Query: 121 RNQSDESSKHFRRQISRQT-TLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSRHNS 180
           R+ S+E+SK  RRQ+ RQT T+NSSSDSS FHYDES++YSS+PD+APVRIMRPQMSRHNS
Sbjct: 121 RHPSEETSKSTRRQMCRQTSTMNSSSDSSAFHYDESSEYSSSPDAAPVRIMRPQMSRHNS 180

Query: 181 IRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAAAA- 240
           +R+SNPMENYTDPTEPDRDNR+RH+  +LS +RM+SAREAVAAQEAADAASAAAAAAAA 
Sbjct: 181 VRVSNPMENYTDPTEPDRDNRIRHL--NLSEHRMMSAREAVAAQEAADAASAAAAAAAAA 240

Query: 241 -AAEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHETD 300
            AAEE+K+Q     NEN NE+ NENE EESTT SEEEE  + P+SEPVRMSLMDLLHETD
Sbjct: 241 EAAEEEKKQ-----NENNNESGNENEEEESTTESEEEEEESSPASEPVRMSLMDLLHETD 300

Query: 301 REMGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGGEFACCVCMVKHKNAPMAPCGHTF 360
           REMGFE +SYGMGF  +F ++DEDD+YEEDEDDG G EF+CCVCMVKHKN P+A CGHTF
Sbjct: 301 REMGFERSSYGMGFEGNFLEEDEDDEYEEDEDDGNGEEFSCCVCMVKHKNGPLASCGHTF 360

Query: 361 CRLCSKELMVSRGNCPTCNDFILEILDAF 387
           CRLCSKELMVSRGNCPTC++FILEILDAF
Sbjct: 361 CRLCSKELMVSRGNCPTCSNFILEILDAF 382

BLAST of Lag0033147 vs. ExPASy TrEMBL
Match: A0A6J1K5D6 (E3 ubiquitin-protein ligase rnf8-A-like OS=Cucurbita maxima OX=3661 GN=LOC111491321 PE=4 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 9.6e-156
Identity = 310/389 (79.69%), Postives = 344/389 (88.43%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPAA--ARAAEIRGRTLLDIIRDEEPGVAKGLFGK 60
           MS++E+GRSLAGYTLDDVLANNR GPPA+  ARAAEIRGRTLLD+IRDEEP  AKGLFGK
Sbjct: 1   MSLLEEGRSLAGYTLDDVLANNRFGPPASATARAAEIRGRTLLDVIRDEEPSAAKGLFGK 60

Query: 61  DKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAGDY 120
           DKRTWKSFR+KLRLKRAGSAWVSTVPIPTSDMP   KRSL+GKR+QVRF+TPSSRN+ D+
Sbjct: 61  DKRTWKSFREKLRLKRAGSAWVSTVPIPTSDMPFQPKRSLMGKRSQVRFNTPSSRNSTDH 120

Query: 121 SNRNQSDESSKHFRRQISRQ-TTLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSRH 180
           S RN S+ESSK FRRQ+SRQ +T+NSSSDS+ F YDES DYSS PDSAP R MRPQMSRH
Sbjct: 121 SARNPSEESSKSFRRQVSRQSSTMNSSSDSTPFQYDESFDYSSVPDSAPSR-MRPQMSRH 180

Query: 181 NSIRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAAA 240
           NSIRI NP ENYTDPTE DRDNRVRH+SS+ S  RM+SARE VAAQEAADA  AAAAAAA
Sbjct: 181 NSIRIPNPTENYTDPTESDRDNRVRHISSAFSERRMMSARETVAAQEAADA--AAAAAAA 240

Query: 241 AAAEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHETD 300
            AAEE+KE+++  E   + ENE+ENEN ESTTGSEE E  + P+SEPVRMSLMDLLHETD
Sbjct: 241 EAAEEEKEKEKEKE---QIENEDENENVESTTGSEEWEKVS-PTSEPVRMSLMDLLHETD 300

Query: 301 REMGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGGEFACCVCMVKHKNAPMAPCGHTF 360
           REMGFEG++YGMGF ED F D+ED  YEEDEDDG+GGEF+CCVCMVKHKNAPMAPCGHTF
Sbjct: 301 REMGFEGSNYGMGFEEDGFSDEEDVAYEEDEDDGSGGEFSCCVCMVKHKNAPMAPCGHTF 360

Query: 361 CRLCSKELMVSRGNCPTCNDFILEILDAF 387
           CRLCSKELMVSRGNCPTCNDFILE+L+AF
Sbjct: 361 CRLCSKELMVSRGNCPTCNDFILEMLEAF 382

BLAST of Lag0033147 vs. ExPASy TrEMBL
Match: A0A6J1ESA4 (uncharacterized protein LOC111437281 OS=Cucurbita moschata OX=3662 GN=LOC111437281 PE=4 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.3e-152
Identity = 305/390 (78.21%), Postives = 340/390 (87.18%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLANNRLGPPAAA--RAAEIRGRTLLDIIRDEEPGVAKGLFGK 60
           M +VE+GRSLAGYTLDDVLANNR GPPA+A  RAAEIRGRTLLD+IRDEEP  AKGLFGK
Sbjct: 1   MPLVEEGRSLAGYTLDDVLANNRFGPPASATSRAAEIRGRTLLDVIRDEEPNAAKGLFGK 60

Query: 61  DKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAGDY 120
           DKRTWKSFR+KLRLKRAGSAWVSTVPIPTSDMP   KRSL+GKR+QVRF+TPSSRN+ D+
Sbjct: 61  DKRTWKSFREKLRLKRAGSAWVSTVPIPTSDMPFQPKRSLMGKRSQVRFNTPSSRNSTDH 120

Query: 121 SNRNQSDESSKHFRRQISRQ-TTLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSRH 180
           S RN S+ESSK  RRQ+SRQ +T+NSSSDS+ F YDES DYSS PDSAP R MRPQMSRH
Sbjct: 121 SARNPSEESSKSIRRQVSRQSSTMNSSSDSTPFQYDESFDYSSIPDSAPSR-MRPQMSRH 180

Query: 181 NSIRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAAA 240
           NSIRI NP ENYTDPTE DRDNRVRH+SS+ S  RM+SARE VAAQEAADAA+AAAAA A
Sbjct: 181 NSIRIPNPTENYTDPTESDRDNRVRHISSAFSERRMMSARETVAAQEAADAAAAAAAAEA 240

Query: 241 AAAEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHETD 300
           A  E++KE++       +NENE+E+EN ESTTGSEE E  + P+SEPVRMSLMDLLHETD
Sbjct: 241 AEEEKEKEKE-------QNENEDEDENVESTTGSEEGEKES-PTSEPVRMSLMDLLHETD 300

Query: 301 REMGFEGASYGMGFGEDFFDDDEDDDY-EEDEDDGTGGEFACCVCMVKHKNAPMAPCGHT 360
           REMGFEG++YGMGF ED F D+ED  Y EEDEDDG+GGEF+CCVCMVKHKNAPMA CGHT
Sbjct: 301 REMGFEGSNYGMGFEEDGFSDEEDVAYEEEDEDDGSGGEFSCCVCMVKHKNAPMAACGHT 360

Query: 361 FCRLCSKELMVSRGNCPTCNDFILEILDAF 387
           FCRLCSKELMVSRGNCPTCNDFILE+L+AF
Sbjct: 361 FCRLCSKELMVSRGNCPTCNDFILEMLEAF 381

BLAST of Lag0033147 vs. TAIR 10
Match: AT3G07120.1 (RING/U-box superfamily protein )

HSP 1 Score: 201.8 bits (512), Expect = 9.8e-52
Identity = 153/393 (38.93%), Postives = 211/393 (53.69%), Query Frame = 0

Query: 9   SLAGYTLDDVLANNRL-------GPPAAARAAEIRGR----TLLDIIRDEEPGVAKGLFG 68
           SLAG +LD VL    L        PP     + +  R    TL DIIR+E          
Sbjct: 10  SLAGLSLDAVLGGEILIEPPSLPSPPPLPSKSLVPHRPTSQTLFDIIREEYAKEGH---- 69

Query: 69  KDKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAGD 128
           KD+ TW+ FR+KLRLKR GSAW S++ IP SD+ + N + +         S  +  N  D
Sbjct: 70  KDRTTWQIFREKLRLKRTGSAWTSSLHIPASDILIPNPKHI----GTAFRSHSAGLNIRD 129

Query: 129 YSNR-NQSDESSKHFRRQISRQTTLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSR 188
             +    SD      R   +R +++   S  +    D+S D S   D  P R  +PQ+SR
Sbjct: 130 LVHAIPMSDPPGSSGRAMFTRGSSMRVGSSKNP---DDSPDISVLEDGPPSRSFKPQLSR 189

Query: 189 HNSIRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAA 248
           H+S+R         D +E + +NR RH   +    R +SAREAVAAQEAA+A +AAA   
Sbjct: 190 HDSVR---------DHSEGEENNRRRHPIVTFVEERQMSAREAVAAQEAAEAEAAAAGG- 249

Query: 249 AAAAEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVR-MSLMDLLHE 308
                                +E+E++++E     E EE+ +  +SEP + MSLMDLL E
Sbjct: 250 ---------------------SEDEDDDDEEDDSGETEEMKSSSASEPKQTMSLMDLLEE 309

Query: 309 TDREMGFEGASYGMGFGEDFFDDDEDDDYEEDEDD--GTGGEFACCVCMVKHKNAPMAPC 368
           TDR+MG  G+ Y M   E++ +D+ED++ EE+ D   G  GE +CCVCMVK K A   PC
Sbjct: 310 TDRQMGLTGSRYAMDEDEEYEEDEEDENNEEEGDSHGGGEGELSCCVCMVKIKGASFTPC 360

Query: 369 GHTFCRLCSKELMVSRGNCPTCNDFILEILDAF 387
           GHTFC+LCSKELM  +G+CP C+ F+LE L+ F
Sbjct: 370 GHTFCKLCSKELMAQKGHCPVCSSFVLEFLEIF 360

BLAST of Lag0033147 vs. TAIR 10
Match: AT3G25030.1 (RING/U-box superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 4.4e-20
Identity = 105/392 (26.79%), Postives = 135/392 (34.44%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLA--NNRLGP----PAAARAAEIRGRTLLDIIRDEEPGVAKG 60
           MS VE  RS    TL+ +L    NR  P    P  +R+     R+L+++++ E       
Sbjct: 18  MSTVESSRSC--LTLEAILMADKNRTSPQILSPPPSRSQS--NRSLVEVMQREH------ 77

Query: 61  LFGKDKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRN 120
              +DK  WKS R+KLRLKR GS W+S+  IP+ D P+                      
Sbjct: 78  RHSRDKTAWKSLREKLRLKRNGSVWISSNSIPSLDTPI---------------------- 137

Query: 121 AGDYSNRNQSDESSKHFRRQISRQTTLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQM 180
                                                                       
Sbjct: 138 ------------------------------------------------------------ 197

Query: 181 SRHNSIRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAA 240
                               P+RDN    +   LSN            +EA+        
Sbjct: 198 --------------------PNRDNVSHQLGFLLSN-------SGNVTEEASSVEGRIRL 250

Query: 241 AAAAAAEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLH 300
            A  A                         EE    + EEE       EP RMSLM+LL 
Sbjct: 258 GAVLA-------------------------EERALSAREEETPAEREVEPARMSLMELLE 250

Query: 301 ETDREMGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGGEFACCVCMVKHKNAPMAPCG 360
           E + +M F                  D + EE+    T  E +CCVCMV+ K A   PCG
Sbjct: 318 ENEGQMSFVSV---------------DGEAEEEVAAVTAAEISCCVCMVRSKGAAFIPCG 250

Query: 361 HTFCRLCSKELMVSRGNCPTCNDFILEILDAF 387
           HTFCRLCS+EL V RGNCP CN  ILE+LD F
Sbjct: 378 HTFCRLCSRELWVQRGNCPLCNTTILEVLDLF 250

BLAST of Lag0033147 vs. TAIR 10
Match: AT3G25030.2 (RING/U-box superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 4.4e-20
Identity = 105/392 (26.79%), Postives = 135/392 (34.44%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVLA--NNRLGP----PAAARAAEIRGRTLLDIIRDEEPGVAKG 60
           MS VE  RS    TL+ +L    NR  P    P  +R+     R+L+++++ E       
Sbjct: 18  MSTVESSRSC--LTLEAILMADKNRTSPQILSPPPSRSQS--NRSLVEVMQREH------ 77

Query: 61  LFGKDKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRN 120
              +DK  WKS R+KLRLKR GS W+S+  IP+ D P+                      
Sbjct: 78  RHSRDKTAWKSLREKLRLKRNGSVWISSNSIPSLDTPI---------------------- 137

Query: 121 AGDYSNRNQSDESSKHFRRQISRQTTLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQM 180
                                                                       
Sbjct: 138 ------------------------------------------------------------ 197

Query: 181 SRHNSIRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAA 240
                               P+RDN    +   LSN            +EA+        
Sbjct: 198 --------------------PNRDNVSHQLGFLLSN-------SGNVTEEASSVEGRIRL 250

Query: 241 AAAAAAEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLH 300
            A  A                         EE    + EEE       EP RMSLM+LL 
Sbjct: 258 GAVLA-------------------------EERALSAREEETPAEREVEPARMSLMELLE 250

Query: 301 ETDREMGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGGEFACCVCMVKHKNAPMAPCG 360
           E + +M F                  D + EE+    T  E +CCVCMV+ K A   PCG
Sbjct: 318 ENEGQMSFVSV---------------DGEAEEEVAAVTAAEISCCVCMVRSKGAAFIPCG 250

Query: 361 HTFCRLCSKELMVSRGNCPTCNDFILEILDAF 387
           HTFCRLCS+EL V RGNCP CN  ILE+LD F
Sbjct: 378 HTFCRLCSRELWVQRGNCPLCNTTILEVLDLF 250

BLAST of Lag0033147 vs. TAIR 10
Match: AT4G13100.3 (RING/U-box superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 3.5e-17
Identity = 107/401 (26.68%), Postives = 136/401 (33.92%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVL-ANNRLGPPAAARAAEIRGR---TLLDIIRDEEPGVAKGLF 60
           MS VE+GRS    TL+D+L A              +R     +LLD++R E         
Sbjct: 18  MSTVENGRS--SVTLEDILMAETSSFRSLTTPTTPVRNHSSSSLLDVMRRER-------- 77

Query: 61  GKDKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAG 120
            +DK  WKS RDKLRLKR  + W+S+ PIPT D                           
Sbjct: 78  RRDKTAWKSLRDKLRLKRTATGWISSNPIPTLDN-------------------------- 137

Query: 121 DYSNRNQSDESSKHFRRQISRQTTLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSR 180
                                                               I+ P    
Sbjct: 138 ---------------------------------------------------HILTPDNDS 197

Query: 181 HNSIRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAA 240
           H   R+   + N    +E +R +  R VS               AA+EAA+         
Sbjct: 198 HRFNRLGFLLTN----SETNRSS--RDVSD--------------AAEEAAEREGRLRLGT 256

Query: 241 AAAAEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHET 300
             AAE                                         EP RMSLM+LL + 
Sbjct: 258 VLAAER----------------------------------------EPPRMSLMELLEDN 256

Query: 301 DREMGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGG-----------EFACCVCMVKH 360
           D  M               ++    D+ E +  DG  G           E  CCVCMV+ 
Sbjct: 318 DGHM---------------YELSARDEVEVEGRDGCDGGGEAVAVTGAAELGCCVCMVRS 256

Query: 361 KNAPMAPCGHTFCRLCSKELMVSRGNCPTCNDFILEILDAF 387
           K A   PCGHTFCRLCS+EL V RGNCP CN  IL++LD F
Sbjct: 378 KGAAFIPCGHTFCRLCSRELWVQRGNCPLCNTAILQVLDIF 256

BLAST of Lag0033147 vs. TAIR 10
Match: AT4G13100.5 (RING/U-box superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 3.5e-17
Identity = 107/401 (26.68%), Postives = 136/401 (33.92%), Query Frame = 0

Query: 1   MSIVEDGRSLAGYTLDDVL-ANNRLGPPAAARAAEIRGR---TLLDIIRDEEPGVAKGLF 60
           MS VE+GRS    TL+D+L A              +R     +LLD++R E         
Sbjct: 18  MSTVENGRS--SVTLEDILMAETSSFRSLTTPTTPVRNHSSSSLLDVMRRER-------- 77

Query: 61  GKDKRTWKSFRDKLRLKRAGSAWVSTVPIPTSDMPVHNKRSLIGKRNQVRFSTPSSRNAG 120
            +DK  WKS RDKLRLKR  + W+S+ PIPT D                           
Sbjct: 78  RRDKTAWKSLRDKLRLKRTATGWISSNPIPTLDN-------------------------- 137

Query: 121 DYSNRNQSDESSKHFRRQISRQTTLNSSSDSSGFHYDESADYSSAPDSAPVRIMRPQMSR 180
                                                               I+ P    
Sbjct: 138 ---------------------------------------------------HILTPDNDS 197

Query: 181 HNSIRISNPMENYTDPTEPDRDNRVRHVSSSLSNYRMLSAREAVAAQEAADAASAAAAAA 240
           H   R+   + N    +E +R +  R VS               AA+EAA+         
Sbjct: 198 HRFNRLGFLLTN----SETNRSS--RDVSD--------------AAEEAAEREGRLRLGT 256

Query: 241 AAAAEEKKEQDENSENENENENENENENEESTTGSEEEEVTTPPSSEPVRMSLMDLLHET 300
             AAE                                         EP RMSLM+LL + 
Sbjct: 258 VLAAER----------------------------------------EPPRMSLMELLEDN 256

Query: 301 DREMGFEGASYGMGFGEDFFDDDEDDDYEEDEDDGTGG-----------EFACCVCMVKH 360
           D  M               ++    D+ E +  DG  G           E  CCVCMV+ 
Sbjct: 318 DGHM---------------YELSARDEVEVEGRDGCDGGGEAVAVTGAAELGCCVCMVRS 256

Query: 361 KNAPMAPCGHTFCRLCSKELMVSRGNCPTCNDFILEILDAF 387
           K A   PCGHTFCRLCS+EL V RGNCP CN  IL++LD F
Sbjct: 378 KGAAFIPCGHTFCRLCSRELWVQRGNCPLCNTAILQVLDIF 256