Lag0033120 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0033120
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Glycosyltransferase
Location	chr11: 41045187 .. 41047302 (+)
RNA-Seq Expression	Lag0033120
Synteny	Lag0033120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAGCGCACTTGTCTCCTTCCTTCCTTCCAATTCGATATCCGTTCTTTTTTACGGGTTTTTAGGTGCTGGAGTGGATGGAGAAGAAATGAAGGATGAACGCGATCAGACCAAACATACCGTCGATGTACACCGACGGGCGAATAGAGGACGATATACTGACTATGGATTCAACTTCTTCAAACCGAACAGCGACGTTTGCCAAAAATGGCTCGATGATCGTCGCAAAGCGTCGGTGGTTTTCGTGGCGTTTGGGAGCTTTGCAGCTTTGAGTGTTGAGCAGATGGAGGGAATTGGCTTGGGGTTTGGCACAAACCCACTCCTATTTCTTGTGGGTGGTAAGTATTTTTACACTAACCTTATACAAATTCATTCACTGAACAGGTTAAATTACAAGTTTTGGCCATGAAATTTTTAACTTATAGGTCCCAAACTTTAAAATTATGTAACAATCTCAAAATTTCAAATTTGTGTTTAATAGATATTTTTAACTTTGCAACAAGTCCATGAATTTTCAATTAGAAATCCAACTCTCATTCAATATTTTCTGGGTCTGAATTTTGCGTGTAATTATAAAATCCCGTCCTTCTGACTTTATCTAATAAGTTTATGAATTCGGAAAGTATAAGATTGAAAATTCAATGACCTGTTAATTTTTTTTTAAAGTATAGAGATCTATTAGACACAAAATTAAAATTTTCGAGAAATTTTCTTGGTAGTATGTGCTCATTGTAACTAAACTAACACACTTTGGTCAAAATTGTTGGTCATTTTCCACAAAGTTGGGCAACACAATGGAAAGTACCAAACCACGAATCCCTCTTCGCATAGGTCAAGCTACTGATAAATTATAACGCATTTGTACATATTTATTATTTAATGTCAGTTGGATCTTGACCAATGAATGAATGGTGTGGTTTGTGCAAAATTTTCTTGAAGAAATTTTAGGCATGAGGTACAAGGATTGAAATTAAATTTTATTTGTTATATTTAAAATACAAGTTTTTTGTTATCAAAATTTGAATCGTGGAATCAACGGTAAATTTGTAATGTTAGAAATTGAATGTATGACCGCAAAAGAAAAACAACTGGACATCGATGCAGTGCTAAGGCCACACACACTTTGATGCTCAAGTTAGAATCAAGTTTGTAAGTAATCTAAAAGTTTTAGGGTGTGTTTGGTTTAACTTTTCAAGTGTTTAATTTTGAAAATAAATCATTACATATGAGTGTTTAAATTATAAACAATTTTTATACAAAATGTTTTTTAAAAAATGCATTATTGAAAAACATTTAAATTAGAATGTATTCCAAACGGACCCTTAGACTACAAGTAAAAGTGATTAGTTTGAAAGTACGTCTTTCACTATCGTTTCTTTCGTCGTTTTATAAACGAGGATGCTGTAATAGTTAATCATTATCTACCGACATTCCGTCGAGGATCTTGTCGGGTTTATCAGGATGTGATAATTATGGTCCACGGGCTTGTGAATTTTATGTCTATTAGATTTTAAACATTTAATTTTGTATCCAACAGGCTTGTGAATTTTGAAAAATATCGAATAGGTCAAACACTTACACAAAATTGAAAATTAAGGGACCTATTGAATACAAAAGTGAAAATTCGAATACATGTTTTGTACAAAAGTGAAAGTTCGAATACACGTTTCTAAAAGTTTGAGGGACTAGCCATGAAATTAAACGTAAAGAATACGTTTTAGAAGTCTTGTAAAAGATGACAAGAATGGGAACCCATTTAAGAAAATCTGCTTTATAAATAGACCTGCGAGTTCAAATAGAAGCCTGAAATGAATTTGGCACTAAAAATGGCAATGACTCTCTTCTTCATTGTTATAATGTTTGTTTCTTGGCATCAGGTTTCAAATGGTTCTTGCATCAAACAAGAGAAGGAATCTCTGCTCAGGTTCCAAGCAAGTTTTGTTCAATTTTCAATGCAGTTGGCTTCTTGGGATAATGAAACTGATTGCTGCAGTTGGGATGGAGTGGGCTGTGATCCTACCAATGGTCATGTTCTCAAGCTTGATCTTCTAAACTATGAGTACCACCCATTTGAGTATGCCTCTTTCTTGGTTAGTAATGGAGTTGACTTGTAG

mRNA sequence

ATGAACAGCGCACTTGTCTCCTTCCTTCCTTCCAATTCGATATCCGTTCTTTTTTACGGGTTTTTAGGTGCTGGAGTGGATGGAGAAGAAATGAAGGATGAACGCGATCAGACCAAACATACCGTCGATGTACACCGACGGGCGAATAGAGGACGATATACTGACTATGGATTCAACTTCTTCAAACCGAACAGCGACGTTTGCCAAAAATGGCTCGATGATCGTCGCAAAGCGTCGGTGGTTTTCGTGGCGTTTGGGAGCTTTGCAGCTTTGAGTGTTGAGCAGATGGAGGGAATTGGCTTGGGGTTTGGCACAAACCCACTCCTATTTCTTGTTTCAAATGGTTCTTGCATCAAACAAGAGAAGGAATCTCTGCTCAGGTTCCAAGCAAGTTTTGTTCAATTTTCAATGCAGTTGGCTTCTTGGGATAATGAAACTGATTGCTGCAGTTGGGATGGAGTGGGCTGTGATCCTACCAATGGTCATGTTCTCAAGCTTGATCTTCTAAACTATGAGTACCACCCATTTGAGTATGCCTCTTTCTTGGTTAGTAATGGAGTTGACTTGTAG

Coding sequence (CDS)

ATGAACAGCGCACTTGTCTCCTTCCTTCCTTCCAATTCGATATCCGTTCTTTTTTACGGGTTTTTAGGTGCTGGAGTGGATGGAGAAGAAATGAAGGATGAACGCGATCAGACCAAACATACCGTCGATGTACACCGACGGGCGAATAGAGGACGATATACTGACTATGGATTCAACTTCTTCAAACCGAACAGCGACGTTTGCCAAAAATGGCTCGATGATCGTCGCAAAGCGTCGGTGGTTTTCGTGGCGTTTGGGAGCTTTGCAGCTTTGAGTGTTGAGCAGATGGAGGGAATTGGCTTGGGGTTTGGCACAAACCCACTCCTATTTCTTGTTTCAAATGGTTCTTGCATCAAACAAGAGAAGGAATCTCTGCTCAGGTTCCAAGCAAGTTTTGTTCAATTTTCAATGCAGTTGGCTTCTTGGGATAATGAAACTGATTGCTGCAGTTGGGATGGAGTGGGCTGTGATCCTACCAATGGTCATGTTCTCAAGCTTGATCTTCTAAACTATGAGTACCACCCATTTGAGTATGCCTCTTTCTTGGTTAGTAATGGAGTTGACTTGTAG

Protein sequence

MNSALVSFLPSNSISVLFYGFLGAGVDGEEMKDERDQTKHTVDVHRRANRGRYTDYGFNFFKPNSDVCQKWLDDRRKASVVFVAFGSFAALSVEQMEGIGLGFGTNPLLFLVSNGSCIKQEKESLLRFQASFVQFSMQLASWDNETDCCSWDGVGCDPTNGHVLKLDLLNYEYHPFEYASFLVSNGVDL

Homology

[Close]

BLAST of Lag0033120 vs. NCBI nr
Match: XP_038887063.1 (receptor-like protein EIX2 [Benincasa hispida])

HSP 1 Score: 103.2 bits (256), Expect = 2.5e-18
Identity = 52/80 (65.00%), Postives = 61/80 (76.25%), Query Frame = 0


Query: 110 FLVSNGSCIKQEKESLLRFQASFVQFSMQLASWDNETDCCSWDGVGCDPTN-GHVLKLDL 169
           F VS+ SCIKQE+ESLLR +ASFV  S +L SW    DCCSW+GVGCD TN GHV+KLDL
Sbjct: 22  FWVSSASCIKQERESLLRLKASFVDSSNRLVSWKG-NDCCSWEGVGCDHTNGGHVIKLDL 81

Query: 170 LNYEYHPFEYASFLVSNGVD 189
            NYEY    Y++ L+SNGVD
Sbjct: 82  RNYEY---SYSTSLLSNGVD 97

[Close]

BLAST of Lag0033120 vs. NCBI nr
Match: XP_008444420.1 (PREDICTED: LRR receptor-like serine/threonine-protein kinase FLS2 [Cucumis melo] >KAA0053991.1 LRR receptor-like serine/threonine-protein kinase FLS2 [Cucumis melo var. makuwa] >TYK20676.1 LRR receptor-like serine/threonine-protein kinase FLS2 [Cucumis melo var. makuwa])

HSP 1 Score: 99.0 bits (245), Expect = 4.7e-17
Identity = 49/78 (62.82%), Postives = 59/78 (75.64%), Query Frame = 0


Query: 112 VSNGSCIKQEKESLLRFQASFVQFSMQLASWDNETDCCSWDGVGCDPTN-GHVLKLDLLN 171
           +S G CIKQE+ESL+R +ASF+  S +L SW   TDCCSW+GVGCD  N GHV+KLDL N
Sbjct: 26  LSTGLCIKQERESLVRLKASFIDSSNRLGSWKG-TDCCSWEGVGCDRANGGHVVKLDLRN 85

Query: 172 YEYHPFEYASFLVSNGVD 189
           YEY    Y+S L+SNGVD
Sbjct: 86  YEYF---YSSALLSNGVD 99

[Close]

BLAST of Lag0033120 vs. NCBI nr
Match: XP_038887937.1 (receptor-like protein EIX2 [Benincasa hispida])

HSP 1 Score: 94.4 bits (233), Expect = 1.2e-15
Identity = 48/78 (61.54%), Postives = 57/78 (73.08%), Query Frame = 0


Query: 112 VSNGSCIKQEKESLLRFQASFVQFSMQLASWDNETDCCSWDGVGCDPTN-GHVLKLDLLN 171
           VS   CIKQE+E LL+ +ASF+  S +LASW    DCCSW+GVGCD TN GHV+KLDL N
Sbjct: 24  VSTAFCIKQERELLLKLKASFIDSSNRLASWKG-IDCCSWEGVGCDDTNGGHVVKLDLRN 83

Query: 172 YEYHPFEYASFLVSNGVD 189
           YEY     +S L+SNGVD
Sbjct: 84  YEY---SSSSVLLSNGVD 97

[Close]

BLAST of Lag0033120 vs. NCBI nr
Match: XP_022155556.1 (probable leucine-rich repeat receptor-like protein kinase At2g33170 [Momordica charantia])

HSP 1 Score: 91.7 bits (226), Expect = 7.5e-15
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 0


Query: 113 SNGSCIKQEKESLLRFQASFVQFSMQLASWDNETDCCSWDGVGCDPTNGHVLKLDLLNYE 172
           +  SCI++E+E+LL+ +ASF   S +LASW+   DCCSWDGVGC+ +NGHV+KLDL NY+
Sbjct: 19  TTSSCIEEEREALLKLKASFSDSSKRLASWEG-IDCCSWDGVGCNQSNGHVVKLDLRNYD 78

Query: 173 YHPFEYASFLVSNGVD 189
            + F   S LV NG+D
Sbjct: 79  EYNFH--SHLVINGID 91

[Close]

BLAST of Lag0033120 vs. NCBI nr
Match: XP_038888710.1 (LOW QUALITY PROTEIN: receptor-like protein EIX2 [Benincasa hispida])

HSP 1 Score: 90.9 bits (224), Expect = 1.3e-14
Identity = 48/80 (60.00%), Postives = 57/80 (71.25%), Query Frame = 0


Query: 110 FLVSNGSCIKQEKESLLRFQASFVQFSMQLASWDNETDCCSWDGVGCDPTN-GHVLKLDL 169
           + VS  SCIKQE+ESLLR +ASF+  S  LASW    DCCSW GV C+ TN GHV+KLDL
Sbjct: 22  YQVSTASCIKQERESLLRLKASFIDSSNWLASWKG-MDCCSWQGVRCNHTNGGHVVKLDL 81

Query: 170 LNYEYHPFEYASFLVSNGVD 189
            N EY    ++S L+SNGVD
Sbjct: 82  RNREY---TFSSALLSNGVD 97

[Close]

BLAST of Lag0033120 vs. ExPASy Swiss-Prot
Match: K7NBW3 (Mogroside IE synthase OS=Siraitia grosvenorii OX=190515 GN=UGT74AC1 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.4e-08
Identity = 32/104 (30.77%), Postives = 55/104 (52.88%), Query Frame = 0


Query: 56  YGFNFFKPNSDVCQKWLDDRRKASVVFVAFGSFAALSVEQMEGIGLGFGTNPLLFLVSNG 115
           YG + FKPN DVC KWLD +   SV++V++GS   +  EQ++ + LG       FL    
Sbjct: 247 YGLSLFKPNEDVCLKWLDSKPSGSVLYVSYGSLVEMGEEQLKELALGIKETGKFFL---- 306

Query: 116 SCIKQEKESLLRFQASFVQFSMQ---LASWDNETDCCSWDGVGC 157
             + ++ E+  +   +FV+   +   + SW ++ +  +   VGC
Sbjct: 307 -WVVRDTEA-EKLPPNFVESVAEKGLVVSWCSQLEVLAHPSVGC 344

[Close]

BLAST of Lag0033120 vs. ExPASy Swiss-Prot
Match: Q9C9H6 (Receptor-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=RLP11 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.2e-08
Identity = 23/52 (44.23%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 117 CIKQEKESLLRFQASFVQFSMQLASWDNETDCCSWDGVGCDPTNGHVLKLDL 169
           C   +++ LL+F+  F  F  + + W+  TDCCSWDGV CD  +G V+ LDL
Sbjct: 32  CRHDQRDGLLKFRDEFPIFESKSSPWNKTTDCCSWDGVTCDDKSGQVISLDL 83

[Close]

BLAST of Lag0033120 vs. ExPASy Swiss-Prot
Match: Q6VAA6 (UDP-glycosyltransferase 74G1 OS=Stevia rebaudiana OX=55670 GN=UGT74G1 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 7.1e-08
Identity = 38/108 (35.19%), Postives = 54/108 (50.00%), Query Frame = 0


Query: 55  DYGFNFFKPNSDVCQKWLDDRRKASVVFVAFGSFAALSVEQMEGIGLGFGTNPLLFLVSN 114
           D GFN +K N   C  WLDD+ K SVV+VAFGS      EQ+E I      + + FL   
Sbjct: 251 DNGFNLYKANHHECMNWLDDKPKESVVYVAFGSLVKHGPEQVEEITRALIDSDVNFL--- 310

Query: 115 GSCIKQEKESLLRFQASFVQFSMQ--LASWDNETDCCSWDGVGCDPTN 161
              IK ++E  L    S V  + +  + +W  + D  + + VGC  T+
Sbjct: 311 -WVIKHKEEGKLPENLSEVIKTGKGLIVAWCKQLDVLAHESVGCFVTH 354

[Close]

BLAST of Lag0033120 vs. ExPASy Swiss-Prot
Match: Q9SVM3 (Receptor-like protein 49 OS=Arabidopsis thaliana OX=3702 GN=RLP49 PE=2 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 9.2e-08
Identity = 28/68 (41.18%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 108 LLFLVSNG-------SCIKQEKESLLRFQASFVQFSMQLASWDNETDCCSWDGVGCDPTN 167
           L+F +SN         C+  +++SL  F+    +F +    W N TDCCSWDGV CDP  
Sbjct: 19  LIFCLSNSILVFAKHLCLPDQRDSLWGFKN---EFHVPSEKWRNNTDCCSWDGVSCDPKT 78

Query: 168 GHVLKLDL 169
           G+V+ LDL
Sbjct: 79  GNVVGLDL 83

[Close]

BLAST of Lag0033120 vs. ExPASy Swiss-Prot
Match: Q93YT3 (Receptor-like protein 50 OS=Arabidopsis thaliana OX=3702 GN=RLP50 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.7e-07
Identity = 24/71 (33.80%), Postives = 38/71 (53.52%), Query Frame = 0


Query: 109 LFLVSNGSCIKQEKESLLRFQASF---------VQFSMQLASWDNETDCCSWDGVGCDPT 168
           + +++   C+  ++++LL F+  F         +      A W N TDCCSW G+ CDP 
Sbjct: 18  ILVIAKDLCLPDQRDALLEFKNEFSIPSPDSDLMLILQTTAKWRNNTDCCSWGGISCDPK 77

Query: 169 NGHVLKLDLLN 171
            G V++LDL N
Sbjct: 78  TGVVVELDLGN 88

The following BLAST results are available for this feature:

BLAST of Lag0033120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887063.1	2.5e-18	65.00	receptor-like protein EIX2 [Benincasa hispida]	[more]
XP_008444420.1	4.7e-17	62.82	PREDICTED: LRR receptor-like serine/threonine-protein kinase FLS2 [Cucumis melo]...	[more]
XP_038887937.1	1.2e-15	61.54	receptor-like protein EIX2 [Benincasa hispida]	[more]
XP_022155556.1	7.5e-15	55.26	probable leucine-rich repeat receptor-like protein kinase At2g33170 [Momordica c...	[more]
XP_038888710.1	1.3e-14	60.00	LOW QUALITY PROTEIN: receptor-like protein EIX2 [Benincasa hispida]	[more]

BLAST of Lag0033120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
K7NBW3	2.4e-08	30.77	Mogroside IE synthase OS=Siraitia grosvenorii OX=190515 GN=UGT74AC1 PE=1 SV=1	[more]
Q9C9H6	3.2e-08	44.23	Receptor-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=RLP11 PE=3 SV=1	[more]
Q6VAA6	7.1e-08	35.19	UDP-glycosyltransferase 74G1 OS=Stevia rebaudiana OX=55670 GN=UGT74G1 PE=1 SV=1	[more]
Q9SVM3	9.2e-08	41.18	Receptor-like protein 49 OS=Arabidopsis thaliana OX=3702 GN=RLP49 PE=2 SV=2	[more]
Q93YT3	2.7e-07	33.80	Receptor-like protein 50 OS=Arabidopsis thaliana OX=3702 GN=RLP50 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 116..184 e-value: 3.4E-12 score: 48.1
IPR013210	Leucine-rich repeat-containing N-terminal, plant-type	PFAM	PF08263	LRRNT_2	coord: 119..157 e-value: 8.1E-12 score: 45.2
None	No IPR available	PANTHER	PTHR48058:SF8	OSJNBB0089K24.8 PROTEIN	coord: 113..172
None	No IPR available	PANTHER	PTHR48058	LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE FLS2-RELATED	coord: 113..172
None	No IPR available	SUPERFAMILY	53756	UDP-Glycosyltransferase/glycogen phosphorylase	coord: 49..119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0033120.1	Lag0033120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0008194	UDP-glycosyltransferase activity