Lag0033118 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033118
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionGlycosyltransferase
Locationchr11: 41039920 .. 41040525 (+)
RNA-Seq ExpressionLag0033118
SyntenyLag0033118
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACGATGGTGAGGATATTTAAAGAAAACAAGTCTAATTTTCAAAATCCATAAACCAAAAAACAAATGGTTATCAAATTGAGCCTAAATATCGAGAAATTACAAATTTAGAGTTGAAGCTAACGTAGCTGATTGAGACAGGTAAGGGCAGCGGAGGCGGCGAAGCTTCCGGCGAAATTTGCAGAGGAGACGGCGGAGAAAGGGCTATTGGTCCCATGGTGCCCTCAATTGGAGGTTTTATCGCACGAATCGATCGGTTGCTTTGTGACGCACTGCGGCTGGAACTCGACGCTCGAGGCTCTGACCATCGGCGTTCCGATGGTGGCGATGCCGCAGTGGACGGACCAGACCACCAATGGCAAGTGCGTAATGGACATTTGGAAGACTGGAATTAGGGCTCCGGTCGACGGCGCCGGAATCGTGCGGCGGACGACGGTGGCAGATTGTGTGTTGAAGATCATGGACGGCGACGGAGGAGAGGAGGTTCGGAGAAACGCCGCTAAATGGGGAGTGTTGGCCAAACGGGCGGTGGATCGCGGCGGAAGTTCTAATCGGAATGTTGATGAGATTCTTGCCCAATTGAAGTCCGGTTTGAATCTTTAA

mRNA sequence

ATGAAAACGATGGTAAGGGCAGCGGAGGCGGCGAAGCTTCCGGCGAAATTTGCAGAGGAGACGGCGGAGAAAGGGCTATTGGTCCCATGGTGCCCTCAATTGGAGGTTTTATCGCACGAATCGATCGGTTGCTTTGTGACGCACTGCGGCTGGAACTCGACGCTCGAGGCTCTGACCATCGGCGTTCCGATGGTGGCGATGCCGCAGTGGACGGACCAGACCACCAATGGCAAGTGCGTAATGGACATTTGGAAGACTGGAATTAGGGCTCCGGTCGACGGCGCCGGAATCGTGCGGCGGACGACGGTGGCAGATTGTGTGTTGAAGATCATGGACGGCGACGGAGGAGAGGAGGTTCGGAGAAACGCCGCTAAATGGGGAGTGTTGGCCAAACGGGCGGTGGATCGCGGCGGAAGTTCTAATCGGAATGTTGATGAGATTCTTGCCCAATTGAAGTCCGGTTTGAATCTTTAA

Coding sequence (CDS)

ATGAAAACGATGGTAAGGGCAGCGGAGGCGGCGAAGCTTCCGGCGAAATTTGCAGAGGAGACGGCGGAGAAAGGGCTATTGGTCCCATGGTGCCCTCAATTGGAGGTTTTATCGCACGAATCGATCGGTTGCTTTGTGACGCACTGCGGCTGGAACTCGACGCTCGAGGCTCTGACCATCGGCGTTCCGATGGTGGCGATGCCGCAGTGGACGGACCAGACCACCAATGGCAAGTGCGTAATGGACATTTGGAAGACTGGAATTAGGGCTCCGGTCGACGGCGCCGGAATCGTGCGGCGGACGACGGTGGCAGATTGTGTGTTGAAGATCATGGACGGCGACGGAGGAGAGGAGGTTCGGAGAAACGCCGCTAAATGGGGAGTGTTGGCCAAACGGGCGGTGGATCGCGGCGGAAGTTCTAATCGGAATGTTGATGAGATTCTTGCCCAATTGAAGTCCGGTTTGAATCTTTAA

Protein sequence

MKTMVRAAEAAKLPAKFAEETAEKGLLVPWCPQLEVLSHESIGCFVTHCGWNSTLEALTIGVPMVAMPQWTDQTTNGKCVMDIWKTGIRAPVDGAGIVRRTTVADCVLKIMDGDGGEEVRRNAAKWGVLAKRAVDRGGSSNRNVDEILAQLKSGLNL
Homology
BLAST of Lag0033118 vs. NCBI nr
Match: XP_022157619.1 (UDP-glycosyltransferase 74F2-like [Momordica charantia])

HSP 1 Score: 261.9 bits (668), Expect = 3.5e-66
Identity = 121/153 (79.08%), Postives = 135/153 (88.24%), Query Frame = 0

Query: 4   MVRAAEAAKLPAKFAEETAEKGLLVPWCPQLEVLSHESIGCFVTHCGWNSTLEALTIGVP 63
           +VRA E  KLP KFAEETAEKGLLV WCPQLE+LSHESIGCFVTHCGWNSTLEALTIGVP
Sbjct: 312 VVRAKEEEKLPTKFAEETAEKGLLVSWCPQLEILSHESIGCFVTHCGWNSTLEALTIGVP 371

Query: 64  MVAMPQWTDQTTNGKCVMDIWKTGIRAPVDGAGIVRRTTVADCVLKIMDGDGGEEVRRNA 123
           MVAMPQWTDQTTN KC+MDIWKTGIRAP D +GIVRR  VA+C+ +IM+GDGG+E+RRNA
Sbjct: 372 MVAMPQWTDQTTNAKCIMDIWKTGIRAPADNSGIVRRAAVAECIGEIMEGDGGKEIRRNA 431

Query: 124 AKWGVLAKRAVDRGGSSNRNVDEILAQLKSGLN 157
           AKWG LA++AV RGGSS+ NVD+ILA L S  N
Sbjct: 432 AKWGALARQAVGRGGSSDMNVDQILADLASAFN 464

BLAST of Lag0033118 vs. NCBI nr
Match: KAG6571601.1 (UDP-glycosyltransferase 74G1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 257.7 bits (657), Expect = 6.6e-65
Identity = 122/150 (81.33%), Postives = 135/150 (90.00%), Query Frame = 0

Query: 4   MVRAAEAAKLPAKFAEETAEKGLLVPWCPQLEVLSHESIGCFVTHCGWNSTLEALTIGVP 63
           +VR  EAAK+PAKFAE TA++GLLVPWC QL+VLSHESIGCFVTHCGWNSTLEALTIGVP
Sbjct: 314 VVRETEAAKVPAKFAEATADRGLLVPWCRQLDVLSHESIGCFVTHCGWNSTLEALTIGVP 373

Query: 64  MVAMPQWTDQTTNGKCVMDIWKTGIRAPVDGAGIVRRTTVADCVLKIMDGDGGEEVRRNA 123
           MVAMPQWTDQTTN K V DIWKTG+RAP+D AGIVRRTTVADC+LK+MD DGG E+R+NA
Sbjct: 374 MVAMPQWTDQTTNAKFVTDIWKTGVRAPLDDAGIVRRTTVADCILKVMDDDGGTEIRKNA 433

Query: 124 AKWGVLAKRAVDRGGSSNRNVDEILAQLKS 154
           AKWG LAK+AVD  GSS+R VDEILAQL S
Sbjct: 434 AKWGELAKQAVDCDGSSDRTVDEILAQLVS 463

BLAST of Lag0033118 vs. NCBI nr
Match: XP_022967252.1 (UDP-glycosyltransferase 74G1-like [Cucurbita maxima])

HSP 1 Score: 257.3 bits (656), Expect = 8.6e-65
Identity = 122/150 (81.33%), Postives = 134/150 (89.33%), Query Frame = 0

Query: 4   MVRAAEAAKLPAKFAEETAEKGLLVPWCPQLEVLSHESIGCFVTHCGWNSTLEALTIGVP 63
           +VR  EAAK+PAKFAE  A++GLLVPWC QL+VLSHESIGCFVTHCGWNSTLEALTIGVP
Sbjct: 315 VVRETEAAKIPAKFAEAMADRGLLVPWCRQLDVLSHESIGCFVTHCGWNSTLEALTIGVP 374

Query: 64  MVAMPQWTDQTTNGKCVMDIWKTGIRAPVDGAGIVRRTTVADCVLKIMDGDGGEEVRRNA 123
           MVAMPQWTDQTTN K V DIWKTG+RAP+D AGIVRRTTVADC+LK+MD DGG E+R+NA
Sbjct: 375 MVAMPQWTDQTTNAKFVTDIWKTGVRAPLDDAGIVRRTTVADCILKVMDDDGGTEIRKNA 434

Query: 124 AKWGVLAKRAVDRGGSSNRNVDEILAQLKS 154
           AKW  LAKRAVDR GSS+R VDEILAQL S
Sbjct: 435 AKWEELAKRAVDRDGSSDRTVDEILAQLVS 464

BLAST of Lag0033118 vs. NCBI nr
Match: XP_022963519.1 (UDP-glycosyltransferase 74G1-like [Cucurbita moschata])

HSP 1 Score: 256.1 bits (653), Expect = 1.9e-64
Identity = 121/150 (80.67%), Postives = 135/150 (90.00%), Query Frame = 0

Query: 4   MVRAAEAAKLPAKFAEETAEKGLLVPWCPQLEVLSHESIGCFVTHCGWNSTLEALTIGVP 63
           +VR  EAAK+PAKFAE TA++GLLVPWC QL+VLSHESIGCFVTHCGWNSTLEALTIGVP
Sbjct: 316 VVRETEAAKIPAKFAEATADRGLLVPWCRQLDVLSHESIGCFVTHCGWNSTLEALTIGVP 375

Query: 64  MVAMPQWTDQTTNGKCVMDIWKTGIRAPVDGAGIVRRTTVADCVLKIMDGDGGEEVRRNA 123
           MVAMPQWTDQTTN K V DIWKTG+RAP+D AGIVRRTTVADC+LK+MD +GG E+R+NA
Sbjct: 376 MVAMPQWTDQTTNAKFVTDIWKTGVRAPLDDAGIVRRTTVADCILKVMDDNGGTEIRKNA 435

Query: 124 AKWGVLAKRAVDRGGSSNRNVDEILAQLKS 154
           AKWG LAK+AVD  GSS+R VDEILAQL S
Sbjct: 436 AKWGELAKQAVDCDGSSDRTVDEILAQLVS 465

BLAST of Lag0033118 vs. NCBI nr
Match: KAG6571602.1 (UDP-glycosyltransferase 74F2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 255.8 bits (652), Expect = 2.5e-64
Identity = 121/150 (80.67%), Postives = 134/150 (89.33%), Query Frame = 0

Query: 4   MVRAAEAAKLPAKFAEETAEKGLLVPWCPQLEVLSHESIGCFVTHCGWNSTLEALTIGVP 63
           +VR  EAAK+PAKFAE TA++GLLVPWC QL+VLSHESIGCFVTHCGWNSTLEALTIGVP
Sbjct: 316 VVRETEAAKIPAKFAEATADRGLLVPWCRQLDVLSHESIGCFVTHCGWNSTLEALTIGVP 375

Query: 64  MVAMPQWTDQTTNGKCVMDIWKTGIRAPVDGAGIVRRTTVADCVLKIMDGDGGEEVRRNA 123
           MVAMPQWTDQTTN K V DIWKTG+RAP+  AGIVRRTTVADC+L++MD D G E+R+NA
Sbjct: 376 MVAMPQWTDQTTNAKFVTDIWKTGVRAPLGDAGIVRRTTVADCILRVMDDDRGTEIRKNA 435

Query: 124 AKWGVLAKRAVDRGGSSNRNVDEILAQLKS 154
           AKWG LAKRAVDR GSS+R VDEILAQL S
Sbjct: 436 AKWGELAKRAVDRDGSSDRTVDEILAQLVS 465

BLAST of Lag0033118 vs. ExPASy Swiss-Prot
Match: Q9SYK9 (UDP-glycosyltransferase 74E2 OS=Arabidopsis thaliana OX=3702 GN=UGT74E2 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 4.2e-46
Identity = 79/146 (54.11%), Postives = 109/146 (74.66%), Query Frame = 0

Query: 4   MVRAAEAAKLPAKFAEETAEKGLLVPWCPQLEVLSHESIGCFVTHCGWNSTLEALTIGVP 63
           +VR  E  KLP  + EE  EKGL+V W PQL+VL+H+SIGCF+THCGWNSTLE L++GVP
Sbjct: 305 VVRETETHKLPRNYVEEIGEKGLIVSWSPQLDVLAHKSIGCFLTHCGWNSTLEGLSLGVP 364

Query: 64  MVAMPQWTDQTTNGKCVMDIWKTGIRAPVDGAGIVRRTTVADCVLKIMDGDGGEEVRRNA 123
           M+ MP WTDQ TN K + D+WK G+R   +G G VRR  +   V ++M+G+ G+E+R+NA
Sbjct: 365 MIGMPHWTDQPTNAKFMQDVWKVGVRVKAEGDGFVRREEIMRSVEEVMEGEKGKEIRKNA 424

Query: 124 AKWGVLAKRAVDRGGSSNRNVDEILA 150
            KW VLA+ AV  GGSS+++++E ++
Sbjct: 425 EKWKVLAQEAVSEGGSSDKSINEFVS 450

BLAST of Lag0033118 vs. ExPASy Swiss-Prot
Match: Q9SKC1 (UDP-glycosyltransferase 74C1 OS=Arabidopsis thaliana OX=3702 GN=UGT74C1 PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.2e-45
Identity = 82/149 (55.03%), Postives = 111/149 (74.50%), Query Frame = 0

Query: 5   VRAAEAAKLPAKFAEETAEK--GLLVPWCPQLEVLSHESIGCFVTHCGWNSTLEALTIGV 64
           VR +E +KLP+ F EE  EK  GL+  W PQLEVL+HESIGCFV+HCGWNSTLEAL +GV
Sbjct: 308 VRESERSKLPSGFIEEAEEKDSGLVAKWVPQLEVLAHESIGCFVSHCGWNSTLEALCLGV 367

Query: 65  PMVAMPQWTDQTTNGKCVMDIWKTGIRAPVDGAGIVRRTTVADCVLKIMDGDGGEEVRRN 124
           PMV +PQWTDQ TN K + D+WK G+R   DG G+  +  +A C++++M+G+ G+E+R+N
Sbjct: 368 PMVGVPQWTDQPTNAKFIEDVWKIGVRVRTDGEGLSSKEEIARCIVEVMEGERGKEIRKN 427

Query: 125 AAKWGVLAKRAVDRGGSSNRNVDEILAQL 152
             K  VLA+ A+  GGSS++ +DE +A L
Sbjct: 428 VEKLKVLAREAISEGGSSDKKIDEFVALL 456

BLAST of Lag0033118 vs. ExPASy Swiss-Prot
Match: P0C7P7 (UDP-glycosyltransferase 74E1 OS=Arabidopsis thaliana OX=3702 GN=UGT74E1 PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-44
Identity = 80/146 (54.79%), Postives = 104/146 (71.23%), Query Frame = 0

Query: 4   MVRAAEAAKLPAKFAEETAEKGLLVPWCPQLEVLSHESIGCFVTHCGWNSTLEALTIGVP 63
           +VR  E  KLP  + EE  EKGL V W PQLEVL+H+SIGCFVTHCGWNSTLE L++GVP
Sbjct: 305 VVRETERRKLPENYIEEIGEKGLTVSWSPQLEVLTHKSIGCFVTHCGWNSTLEGLSLGVP 364

Query: 64  MVAMPQWTDQTTNGKCVMDIWKTGIRAPVDGAGIVRRTTVADCVLKIMDGDGGEEVRRNA 123
           M+ MP W DQ TN K + D+WK G+R   D  G VRR      V ++M+ + G+E+R+NA
Sbjct: 365 MIGMPHWADQPTNAKFMEDVWKVGVRVKADSDGFVRREEFVRRVEEVMEAEQGKEIRKNA 424

Query: 124 AKWGVLAKRAVDRGGSSNRNVDEILA 150
            KW VLA+ AV  GGSS++N++E ++
Sbjct: 425 EKWKVLAQEAVSEGGSSDKNINEFVS 450

BLAST of Lag0033118 vs. ExPASy Swiss-Prot
Match: A0A0A6ZFR4 (UDP-glucosyltransferase 74AE2 OS=Panax ginseng OX=4054 GN=UGT74AE2 PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 3.9e-44
Identity = 79/145 (54.48%), Postives = 110/145 (75.86%), Query Frame = 0

Query: 9   EAAKLPAKFAEETAEKGLLVPWCPQLEVLSHESIGCFVTHCGWNSTLEALTIGVPMVAMP 68
           E  KL + F  E  EKGL+V WCPQLEVLSH+SIG F+THCGWNST+EAL++GVPMVA+P
Sbjct: 306 ERTKLSSDFLAEAEEKGLIVSWCPQLEVLSHKSIGSFMTHCGWNSTVEALSLGVPMVAVP 365

Query: 69  QWTDQTTNGKCVMDIWKTGIRAPVDGAGIVRRTTVADCVLKIMDGDGGEEVRRNAAKWGV 128
           Q  DQ  N K ++D+W+ G+R P+   G+V R  VA+C+  +M+G+ G+E+R NA KW  
Sbjct: 366 QQFDQPVNAKYIVDVWQIGVRVPIGEDGVVLRGEVANCIKDVMEGEIGDELRGNALKWKG 425

Query: 129 LAKRAVDRGGSSNRNVDEILAQLKS 154
           LA  A+++GGSS++N+DE +++L S
Sbjct: 426 LAVEAMEKGGSSDKNIDEFISKLVS 450

BLAST of Lag0033118 vs. ExPASy Swiss-Prot
Match: K7NBW3 (Mogroside IE synthase OS=Siraitia grosvenorii OX=190515 GN=UGT74AC1 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 7.3e-43
Identity = 78/149 (52.35%), Postives = 102/149 (68.46%), Query Frame = 0

Query: 4   MVRAAEAAKLPAKFAEETAEKGLLVPWCPQLEVLSHESIGCFVTHCGWNSTLEALTIGVP 63
           +VR  EA KLP  F E  AEKGL+V WC QLEVL+H S+GCF THCGWNSTLEAL +GVP
Sbjct: 304 VVRDTEAEKLPPNFVESVAEKGLVVSWCSQLEVLAHPSVGCFFTHCGWNSTLEALCLGVP 363

Query: 64  MVAMPQWTDQTTNGKCVMDIWKTGIRAPVDGAGIVRRTTVADCVLKIMDGDGGEEVRRNA 123
           +VA PQW DQ TN K + D+WK G R   +   +  +  V  C+ ++M+G+   E + N+
Sbjct: 364 VVAFPQWADQVTNAKFLEDVWKVGKRVKRNEQRLASKEEVRSCIWEVMEGERASEFKSNS 423

Query: 124 AKWGVLAKRAVDRGGSSNRNVDEILAQLK 153
            +W   AK AVD GGSS++N++E +A LK
Sbjct: 424 MEWKKWAKEAVDEGGSSDKNIEEFVAMLK 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022157619.13.5e-6679.08UDP-glycosyltransferase 74F2-like [Momordica charantia][more]
KAG6571601.16.6e-6581.33UDP-glycosyltransferase 74G1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022967252.18.6e-6581.33UDP-glycosyltransferase 74G1-like [Cucurbita maxima][more]
XP_022963519.11.9e-6480.67UDP-glycosyltransferase 74G1-like [Cucurbita moschata][more]
KAG6571602.12.5e-6480.67UDP-glycosyltransferase 74F2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9SYK94.2e-4654.11UDP-glycosyltransferase 74E2 OS=Arabidopsis thaliana OX=3702 GN=UGT74E2 PE=1 SV=... [more]
Q9SKC11.2e-4555.03UDP-glycosyltransferase 74C1 OS=Arabidopsis thaliana OX=3702 GN=UGT74C1 PE=2 SV=... [more]
P0C7P71.3e-4454.79UDP-glycosyltransferase 74E1 OS=Arabidopsis thaliana OX=3702 GN=UGT74E1 PE=3 SV=... [more]
A0A0A6ZFR43.9e-4454.48UDP-glucosyltransferase 74AE2 OS=Panax ginseng OX=4054 GN=UGT74AE2 PE=1 SV=1[more]
K7NBW37.3e-4352.35Mogroside IE synthase OS=Siraitia grosvenorii OX=190515 GN=UGT74AC1 PE=1 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002213UDP-glucuronosyl/UDP-glucosyltransferasePFAMPF00201UDPGTcoord: 14..84
e-value: 6.4E-18
score: 64.8
IPR002213UDP-glucuronosyl/UDP-glucosyltransferaseCDDcd03784GT1_Gtf-likecoord: 12..143
e-value: 6.07578E-38
score: 131.519
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 4..132
e-value: 3.2E-63
score: 216.5
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 133..144
e-value: 3.2E-63
score: 216.5
NoneNo IPR availablePANTHERPTHR11926GLUCOSYL/GLUCURONOSYL TRANSFERASEScoord: 4..151
NoneNo IPR availablePANTHERPTHR11926:SF1327GLYCOSYLTRANSFERASEcoord: 4..151
NoneNo IPR availableSUPERFAMILY53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 8..149
IPR035595UDP-glycosyltransferase family, conserved sitePROSITEPS00375UDPGTcoord: 30..73

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033118.1Lag0033118.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0080043 quercetin 3-O-glucosyltransferase activity
molecular_function GO:0080044 quercetin 7-O-glucosyltransferase activity
molecular_function GO:0008194 UDP-glycosyltransferase activity