Lag0033026 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033026
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionbeta-amyrin 11-oxidase-like
Locationchr11: 40069601 .. 40075125 (-)
RNA-Seq ExpressionLag0033026
SyntenyLag0033026
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTTTGGCTACGCCTGATCGGAGCTTTCGCCGGAGCTTACATTTTTGTCTTTGGGATTCTCCGAAACCTTAACCAATGGCGTTTTGTCACTCGACACGGCGGCCGTTACAACCTCCCTCCCGGCGATATGGGCTGGCCGATCATCGGAACCCTCCTCCCCTTTCTTCATGCTTTCCGATCGGGTCGACCCGATTCCTTCATCCACCACTTTGCCTCCAAGTAAATCTCTCTTTTTCTTTTCTCATTATTCTCTCGCCGGATTTTTCGTCGGATCCTAATTATTCTCTCTTTCCATTTTCTGTTTTTTTTGGGTTAAAAATTAAAATAGTTTTGATATACTTTTAAATATCCCAATCAAATTCAGTGGGTTTTATTTTACCCAATACCTTTTTCTACAGTGGTCTATGGTTAGTTTAGTGTTAATTTTTTGGACTTCCATGCCGTGTTCTTTGAATGAAAATTATTGTTATTATTTTATAAGTTTGATAGAAATATTTTAAAATTGACTGGAAGTTGACTAAAATATAATAAGATTCAAACTACAAACTAATATGCTAAAGCGAGCGTAGCTCAGCAAAAATTAATTGACATGGCTGGAATGTTTCAAGATCTATTTTTTGAGTATTTTTTTTTTAATTTAAGATAAATTAGGAGGCAGTAACTCTTTTTTTTACTTCTCACCTCTCACATCAAAAAGAAAAATGTTTTTAGAAGTTAATTGTCTATGGGTCGGAACTACTTAATTAAAAGTAAAGCCTAACTCTCTATTTTCACATTTCAAAAAAGGTATTTTAACTTTTCTTTTTCTTTTTTAATCTATAATTGTGGATTAAAAAAAATTGCATTTTTTAATAGAAATCAAACGAAAACAGCCCCTTTTCAAAAGTTTAGGAAGTTGTACAAATAACATTCAAATAAAATCTCATATAACTTGATGCACTAAACTTATAAATTATTTTTTTAATTTTAAATTGTTGGTTTAGAAAAAAAAAAAAAACTTTTTAAAATAGATTTCAAACAAAACCCAACATTATTTCAAAGACCTATTAGACACCAACTTAAAGTTAAAAGAAAAAAAAAAAACTACAATTTACCCCTTTTGTTGATCAAATATATATATTTTTTTTTTAAAAAAAAAGATAAAAATTTAATGACAAAAAAATGGATGAAATTTTCAGGTATGGGAAAGTGGGGATGTACAAAACCCACTTATTTGGGAGCCCAAGCGTGATCGTGTGCGTGCCGGAAGTGTGCCGGCACGTGCTAATGAACGACGAACAGTTCGTTTTCGGTTACTCTAAAGCCACCAGAATCCTCACCGGAAGCAAGGCTTTGAACACCGTCCCCAAGGCGGAGCACCGCCGTCTACGGCGCCTGATAGCGTCGCTTATAAGCGGCAACGAGGCCCTGTCCATCTACATAGGGCACGTGGAGGGGATCGTCGTCACGTGCCTGGAGGAGTGGAGGACGATGAAGAAGCCGGTGGAATTCTTGTCGGAGATGAAAACGGTGGCGTTTAAGGTTCTTTTACACATCTTCATCGGAGCCAACACGGCGGCTTTCATCGACAAGATGGAGAAGCTCTACACTGACTTCCACTTGGGTTTCATGTCCACCGCCGTCGATCTCCCCGGAACTACCTTTAGCCGAGCTCTCAAGGTAATTTTCCAATTTTAGGAAGATCCGACAAAGTTAATAGGTTTCTTATCCACTAAACTAAATAAACACTAATATATATATATATAAAGAAATTTAAGAATCCGTTTGAAATACATTTTAGTTTGAGTGTTTTTCAAGAATGCATTTTTTCTTAAATATTTTATATAAAAATTGTTTAGAATTTAAACGCTCATGAATGCTTTTATACAAGATATTATATACTTAAAAATTCTTTTTAAAAAATTTGCTTAATCGTTTTCAAAATTAAACCCTTAAAAGTTAAACCAAACACATTATTAGTTTTGAGTATAGAAATTCAAATCTACATCTTTTAAAAGATATGGAGATACTTTAATTATTTACATGTATAAATTAGCTCGATAGGTAGTATTCTCTATCATCTTTAATAAGGTTGAAAGTTTGATTATGTTGACACATTTATTGAAAAAAAAAATCTTTTAAAGATTTTTAAATCTTTGAGTTATATATAATAATTTTAGTTTTATAGATATACTTTTGAAAGTTAAAAGGTTACCAATTCAAATTACTAAATAAAGATAAGATTAAATATTTGTTGAATAATAATTAATAAAAATTTTAAAATGAAATATTTTGAAAGTTTATAGATGAGTTGAATCTATACACTAATTTATAATATAGTTCTAAATTTAAGGTGCTAGAATAATATGTAAACTTGATTTTACTTAAAATAGTTGATTTTAAAATAATTTATTTTGCCCTAGTTTTTATACTTTTAGATGTATATATATTTTTTTAGTTCACTTTTAAATGCATATTTTAAATTGTCAAAAAATATTGTATATTTTAAAACTTCATACAACATTTTGATTTAACATAAATTTCTATAATCTATTTTTTATGCAACTTGTAATTTAAACTACGTGTATTTAGCCTAATATTGAATTTTTAAAAAGATAACACATAACCTTTATGTTAATTAATTTGTATAAAATTAGAGTATGATAAAACAGAACACGTCCAAAATCAAAGTGTGACTTTGTTGTCAAACACTAACTTAAAAATTAAGTCTTCTTTATTTTAAAATAAATAAAGTTCAAAAGAAAAATAAATTTATATATATATATATATATATATATATATATATATATATATATTTGTGTTTGAAACCAAATATTTTATGTATGTCTAAGTTTGAGTTGTGTACTTCACCGAACGTGACAAAACTTAATATGCACATAAAAGAAAAGGAAAAAAAAAGAATTCAAATAATATATATATTATTTATGCTTAATATGCACATAAATATTTTATTTTATTTTATTTTTTTTTTGTAAATTTCAGGCAAGAAATGAGTTGATAAGGATCTTTGAAAATGTATTGAAAGAGAAGAGGGCAAATTTGAAGAACAAAGTGACAGAAAATAGAAAAAAAGATATGACTGATTTGTTATTAGAAGTTAGAGATGAAGATGGGCAAGGCTTGGATGATGGTTGCATTATTGATTTGTTGATTGGTTTTTTCTTTGCTGGCCATGAAACTTCTGCCCATAGTATCATGAGGGCTATCATGTTCCTCTCCGAAAATCCCGAAACCCTTCTCAAAGCTAAGGTTCGTCCTCCTGTCTATAAAAAAAAAATGTATGTATATATGTCGGTTCGATATCTAAAAGTTGATTACATAATCAAGAAAAAAAAATTGACCATTATGTTTGGATTCTAATATGCATGTTGATATTGAATTTTTATGAGTGTAGAATTAGGTCGTTCCTCGAAATAATTTTTCGAGCAATTACTCTATTTCCTTTCATTGGATTGACTTGCTGAAATTTAGTTCAACAAGAAACGGAAGTTTATGGAGTTTGGTGATCTTAAAAAGAAAATATCAAACAAAAAAGCTTGGACTGATTCATATTAATAAACATTAAAACATGTACTTGAAATTGAAACATTGATAATGACACATTTGAAAAGTATATCTTTATTACTTATTAAACAACTATTAATCTAAATGAATATGAAATAAAAACTATGGGAAACTTTACCCAAAATAATAATAATAATAATAATATATATTTTTTTTGAAATAATAATAATAATAATAATAATAATAATAATAATAATAATATATTATTGTGCAGGCTGAACAAGAGCAAATTGTGAAGGCAAGGCCAGCTGATGCCCAACACAAAGGCTTGACCATGAAGGAAATCAAACAAATGGAATATCTTTCTAAGGTGCCCCTCTTTTCTTCTCTATTTACTTTCCAAATATTATAAATTTAACCCCCAATTTTATTAATATTCCTGGGTTTGCCCCAAAAATTTGCCTTCGTTATTTATTATTATTATTATATATTTTTTTGCAAGACTAACGTGTGAAATAATGGCAGATAAACATATTTAAAAAATTAATTTTTTTTTCAACAATAATCTTTTTAAAAAGATATGGTTGTTTTTGTGGCTTAATTAATTAGGTGATCGATGAAACGTTGAGGAAGACAAGTTTGGCATTCACATTGTCAAGAGAGACCAAAGTTGATGTTAACTTAAATGGTTTGTCGGGAGATTCCTATATACGAGTATATGTAGCCCAATATTGTTCATTAAGTAATGGTGTGATTAACTTGTTTTATTTTTCAGGCTATACCATACCAAAAGGATGGAAAATCCTCGTTTGGACTAGAGCGGTTCATATGGATCCTGAAATCTACGAAAATCCACACAAATTCGACCCCTCTAGATGGGATGTAAGTGGTCTCGATTAACTTTTGAGAAAATTTCTAATACTTTCATAGTACATATTCTTACCATTCATATTTTGAATTTTTCTTCCGAAATAATTATTTAAGAAAAATAGTTCTGTGTGTCCATCACTAATTAAGTTTTTCTTAAAAATCTCTTTTTCCTCCCTCTCCTTCCCATCTATAAAAAATTTAGTGGTGAAGCAATAGCAAATTCTTGCTCCCTTATTAGTATAAAATTGTTTATTCATTAAATATTGTTGGGACCCCAAATCATTCTTAAATAATGCTTCCAATTAATAATAAACATAAATAATATAAATTTTCTCGTCAACTCCCTCTCTTGCCTATCTATAAAAAATTTAGTGGTGGGCAACAACAAGTTCGTGCTCCCTAATTAGTACAGAATTGTTTATTCATTAATATTGTTGGAATCCCACATCATTCTTAAGTAATGTTTCTAGTAAATAATGAATAAAAATAGTATCCATACTATTATAATACTAGAAATTTTCTCGTTAACTTTCTCACTCTCTTGTCTGTCTATAAAAAGATTAGTGGTGGACAACAAGAAATTCGTTCTCCCTAATCAGTAGGAAAGTCTTGTTTAGTCTTTAGTGTTGTTAGGAACTTCACATCATTTTTTTAACAACGTTCCCGAATTCCAATAACGAATAGAAGCAGCATCCTTACTACTATGGTATTAGAAAATTTTCTCGTTCACTCTTTATCTATCCTCAATAATATCAAGAAAACATTAGAATAGATGAACTAAAAATCACATATTTTCCTTTTTGGTTTCAGAATCCAAAACGCAGAGCTGGATCTTTCATTCCTTTTGGCGCAGGAATGAGACTTTGCCCTGGAATTGATCTTTCCAAACTCGAAATTGCCATTTTCCTTCACTATTTCATCCATGGTGGCTACAGGTGAGAGATTGAAAACTTTTTTTTTTTTTGTAAGACTAGAAAAAATTTCATTAATTTATGTAAAAATAGTTCATAAATTATGAACAACTTTTTTTTTTTTTTTTTTTTTAAATGCAGACTTGAGAGAGTAAATCCAAACTGCCCAGAGAATTATCTGCCTTTGCCAAGGCCTATAGACAACTGCCTTGCAAGAGTTATAAGAGATTCGTGA

mRNA sequence

ATGGATGTTTGGCTACGCCTGATCGGAGCTTTCGCCGGAGCTTACATTTTTGTCTTTGGGATTCTCCGAAACCTTAACCAATGGCGTTTTGTCACTCGACACGGCGGCCGTTACAACCTCCCTCCCGGCGATATGGGCTGGCCGATCATCGGAACCCTCCTCCCCTTTCTTCATGCTTTCCGATCGGGTCGACCCGATTCCTTCATCCACCACTTTGCCTCCAAGTATGGGAAAGTGGGGATGTACAAAACCCACTTATTTGGGAGCCCAAGCGTGATCGTGTGCGTGCCGGAAGTGTGCCGGCACGTGCTAATGAACGACGAACAGTTCGTTTTCGGTTACTCTAAAGCCACCAGAATCCTCACCGGAAGCAAGGCTTTGAACACCGTCCCCAAGGCGGAGCACCGCCGTCTACGGCGCCTGATAGCGTCGCTTATAAGCGGCAACGAGGCCCTGTCCATCTACATAGGGCACGTGGAGGGGATCGTCGTCACGTGCCTGGAGGAGTGGAGGACGATGAAGAAGCCGGTGGAATTCTTGTCGGAGATGAAAACGGTGGCGTTTAAGGTTCTTTTACACATCTTCATCGGAGCCAACACGGCGGCTTTCATCGACAAGATGGAGAAGCTCTACACTGACTTCCACTTGGGTTTCATGTCCACCGCCGTCGATCTCCCCGGAACTACCTTTAGCCGAGCTCTCAAGGCAAGAAATGAGTTGATAAGGATCTTTGAAAATGTATTGAAAGAGAAGAGGGCAAATTTGAAGAACAAAGTGACAGAAAATAGAAAAAAAGATATGACTGATTTGTTATTAGAAGTTAGAGATGAAGATGGGCAAGGCTTGGATGATGGTTGCATTATTGATTTGTTGATTGGTTTTTTCTTTGCTGGCCATGAAACTTCTGCCCATAGTATCATGAGGGCTATCATGTTCCTCTCCGAAAATCCCGAAACCCTTCTCAAAGCTAAGGCTGAACAAGAGCAAATTGTGAAGGCAAGGCCAGCTGATGCCCAACACAAAGGCTTGACCATGAAGGAAATCAAACAAATGGAATATCTTTCTAAGGTGATCGATGAAACGTTGAGGAAGACAAGTTTGGCATTCACATTGTCAAGAGAGACCAAAGTTGATGTTAACTTAAATGGCTATACCATACCAAAAGGATGGAAAATCCTCGTTTGGACTAGAGCGGTTCATATGGATCCTGAAATCTACGAAAATCCACACAAATTCGACCCCTCTAGATGGGATAATCCAAAACGCAGAGCTGGATCTTTCATTCCTTTTGGCGCAGGAATGAGACTTTGCCCTGGAATTGATCTTTCCAAACTCGAAATTGCCATTTTCCTTCACTATTTCATCCATGGTGGCTACAGACTTGAGAGAGTAAATCCAAACTGCCCAGAGAATTATCTGCCTTTGCCAAGGCCTATAGACAACTGCCTTGCAAGAGTTATAAGAGATTCGTGA

Coding sequence (CDS)

ATGGATGTTTGGCTACGCCTGATCGGAGCTTTCGCCGGAGCTTACATTTTTGTCTTTGGGATTCTCCGAAACCTTAACCAATGGCGTTTTGTCACTCGACACGGCGGCCGTTACAACCTCCCTCCCGGCGATATGGGCTGGCCGATCATCGGAACCCTCCTCCCCTTTCTTCATGCTTTCCGATCGGGTCGACCCGATTCCTTCATCCACCACTTTGCCTCCAAGTATGGGAAAGTGGGGATGTACAAAACCCACTTATTTGGGAGCCCAAGCGTGATCGTGTGCGTGCCGGAAGTGTGCCGGCACGTGCTAATGAACGACGAACAGTTCGTTTTCGGTTACTCTAAAGCCACCAGAATCCTCACCGGAAGCAAGGCTTTGAACACCGTCCCCAAGGCGGAGCACCGCCGTCTACGGCGCCTGATAGCGTCGCTTATAAGCGGCAACGAGGCCCTGTCCATCTACATAGGGCACGTGGAGGGGATCGTCGTCACGTGCCTGGAGGAGTGGAGGACGATGAAGAAGCCGGTGGAATTCTTGTCGGAGATGAAAACGGTGGCGTTTAAGGTTCTTTTACACATCTTCATCGGAGCCAACACGGCGGCTTTCATCGACAAGATGGAGAAGCTCTACACTGACTTCCACTTGGGTTTCATGTCCACCGCCGTCGATCTCCCCGGAACTACCTTTAGCCGAGCTCTCAAGGCAAGAAATGAGTTGATAAGGATCTTTGAAAATGTATTGAAAGAGAAGAGGGCAAATTTGAAGAACAAAGTGACAGAAAATAGAAAAAAAGATATGACTGATTTGTTATTAGAAGTTAGAGATGAAGATGGGCAAGGCTTGGATGATGGTTGCATTATTGATTTGTTGATTGGTTTTTTCTTTGCTGGCCATGAAACTTCTGCCCATAGTATCATGAGGGCTATCATGTTCCTCTCCGAAAATCCCGAAACCCTTCTCAAAGCTAAGGCTGAACAAGAGCAAATTGTGAAGGCAAGGCCAGCTGATGCCCAACACAAAGGCTTGACCATGAAGGAAATCAAACAAATGGAATATCTTTCTAAGGTGATCGATGAAACGTTGAGGAAGACAAGTTTGGCATTCACATTGTCAAGAGAGACCAAAGTTGATGTTAACTTAAATGGCTATACCATACCAAAAGGATGGAAAATCCTCGTTTGGACTAGAGCGGTTCATATGGATCCTGAAATCTACGAAAATCCACACAAATTCGACCCCTCTAGATGGGATAATCCAAAACGCAGAGCTGGATCTTTCATTCCTTTTGGCGCAGGAATGAGACTTTGCCCTGGAATTGATCTTTCCAAACTCGAAATTGCCATTTTCCTTCACTATTTCATCCATGGTGGCTACAGACTTGAGAGAGTAAATCCAAACTGCCCAGAGAATTATCTGCCTTTGCCAAGGCCTATAGACAACTGCCTTGCAAGAGTTATAAGAGATTCGTGA

Protein sequence

MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLSEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELIRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDNCLARVIRDS
Homology
BLAST of Lag0033026 vs. NCBI nr
Match: XP_022995340.1 (beta-amyrin 11-oxidase-like [Cucurbita maxima])

HSP 1 Score: 936.4 bits (2419), Expect = 9.9e-269
Identity = 453/490 (92.45%), Postives = 472/490 (96.33%), Query Frame = 0

Query: 1   MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
           MD+WL LIGA AG Y+FVFGILRNLNQWRFVTRHG RYNLPPGDMGWP+IGTLLPFL AF
Sbjct: 3   MDIWLCLIGALAGVYVFVFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62

Query: 61  RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
           RSGRPDSFIHHF SKYGK GMYKTHLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63  RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122

Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
           LTGSKALN+VPK EHRRLRRLIASLISGNEALS+YIGHVEGIVVTCLEEW +MK+PVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 182

Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
           SEMKTVAFKVLLHIFIGAN+AAFIDKMEKLY DFHLGFMSTAVD+PGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 242

Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
           IRIFE VL+EKR NLKNKV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302

Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
           TSAH+IMRAIMFLSENPETLLKAKAEQE+IV ARPADAQHKGLTMKEIKQMEYLSKV+DE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEKIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362

Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
           TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENPH+FDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHQFDPSRWDNP 422

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLP P D
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482

Query: 481 NCLARVIRDS 491
           NCLARV+RDS
Sbjct: 483 NCLARVVRDS 492

BLAST of Lag0033026 vs. NCBI nr
Match: KAG7036178.1 (Beta-amyrin 11-oxidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 934.9 bits (2415), Expect = 2.9e-268
Identity = 451/490 (92.04%), Postives = 471/490 (96.12%), Query Frame = 0

Query: 1   MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
           MD+W+ LIGA AG Y+F+FGI RNLNQWRFVTRHG RYNLPPGDMGWP+IGTLLPFL AF
Sbjct: 1   MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60

Query: 61  RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
           RSGRPDSFIHHF SKYGK GMY+THLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 61  RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120

Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
           LTGSKALN+VPK EHRRLRRLIASLISGNEALS+YIGHVEGIVVTCLEEW +MK+PVEFL
Sbjct: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180

Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
           SEMKTVAFKVLLHIFIGAN+AAFIDKMEKLY DFHLGFMSTAVD+PGTTFSRALKARNEL
Sbjct: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
           IRIFE VL+EKR NLKNKV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
           TSAH+IMRAIMFLSENPETLLKAKAEQEQIV ARPADAQHKGLTMKEIKQMEYLSKV+DE
Sbjct: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360

Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
           TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENPHKFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLP P D
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480

Query: 481 NCLARVIRDS 491
           NCLARV+RDS
Sbjct: 481 NCLARVVRDS 490

BLAST of Lag0033026 vs. NCBI nr
Match: XP_022931256.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 934.5 bits (2414), Expect = 3.8e-268
Identity = 452/490 (92.24%), Postives = 471/490 (96.12%), Query Frame = 0

Query: 1   MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
           MD+WL LIGA AG Y+F+FGILRNLNQWRFVTRHG RYNLPPGDMGWP+IGTLLPFL AF
Sbjct: 3   MDIWLCLIGALAGVYVFLFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62

Query: 61  RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
           RSGRPDSFIHHF SKYGK GMYKTHLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63  RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122

Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
           LTGSKALN+VPK EHRRLRRLIASLISGNEALS+YI HVEGIVVTCLEEW +MK+PVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIEHVEGIVVTCLEEWGSMKEPVEFL 182

Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
           SEMKTVAFKVLLHIFIGAN+AAFIDKMEKLY DFH+GFMSTAVD+PGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHMGFMSTAVDIPGTTFSRALKARNEL 242

Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
           IRIFE VL+EKR NLKNKV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302

Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
           TSAH+IMRAIMFLSENPETLLKAKAEQEQIV ARPADAQHKGLTMKEIKQMEYLSKV+DE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362

Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
           TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENPHKFDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 422

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLP P D
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482

Query: 481 NCLARVIRDS 491
           NCLARV+RDS
Sbjct: 483 NCLARVVRDS 492

BLAST of Lag0033026 vs. NCBI nr
Match: XP_023554757.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 932.9 bits (2410), Expect = 1.1e-267
Identity = 448/490 (91.43%), Postives = 472/490 (96.33%), Query Frame = 0

Query: 1   MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
           M+VW+ L+GA  GAYIFVF ILRNLNQWRFVTRHGGRYNLPPGDMGWP++GTLLPFL AF
Sbjct: 1   MEVWVWLVGALVGAYIFVFVILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60

Query: 61  RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
           RSGRPDSFIHHFASKYGK+GMYKT+LFGSPSVIVCVPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120

Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
           LTGSKALNTV + EHRR+RRLIASLISGN+ALSIYIGHVEGIV+TCLEEW TMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180

Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
           SEMKT+AFK+LLHIFIGANTAAFIDKMEKLYTDFHLGFMS+AVD+PGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
           IRIFE+VLKEKR NLKNKV ENRKKDMTDLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
           TSAHSIMRAIMFLSENPETL KAKAEQEQIV ARPA+ +H+GLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQEQIVNARPANERHRGLTMKEIKQMEYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
           TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHMDPEIYENP KFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPENYLPLPRP D
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480

Query: 481 NCLARVIRDS 491
           NCLARV+RDS
Sbjct: 481 NCLARVVRDS 490

BLAST of Lag0033026 vs. NCBI nr
Match: XP_022952525.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 931.0 bits (2405), Expect = 4.2e-267
Identity = 446/490 (91.02%), Postives = 472/490 (96.33%), Query Frame = 0

Query: 1   MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
           M+VW+ L+GA  GAYI VFGILRNLNQWRFVTRHGGRYNLPPGDMGWP++GTLLPFL AF
Sbjct: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60

Query: 61  RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
           RSGRPDSFIHHFASKYGK+GMYKT+LFGSPSVIVCVPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120

Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
           LTGSKALNTV + EHRR+RRLIASLISGN+ALSIYIGHVEGIV+TCLEEW TMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180

Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
           SEMKT+AFK+LLHIFIGANTAAFIDKMEKLYTDFHLGFMS+AVD+PGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
           IRIFE+VLKEKR NLKNKV ENRKKDMTDLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
           TSAHSIMRAIMFLSENPETL KAKAEQ+QIV ARP++ +H+GLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPSNERHRGLTMKEIKQMEYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
           TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHMDPEIYENP KFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPENYLPLPRP D
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480

Query: 481 NCLARVIRDS 491
           NCLARV+RDS
Sbjct: 481 NCLARVVRDS 490

BLAST of Lag0033026 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.8e-140
Identity = 247/478 (51.67%), Postives = 339/478 (70.92%), Query Frame = 0

Query: 15  YIFVFGILRNLNQWRF-VTRHGGRYNLPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFA 74
           YIF    +RNLN W + V      + LPPGDMGWP+IG LL F+  F SG PDSFI++  
Sbjct: 17  YIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFINNLV 76

Query: 75  SKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNTVPKA 134
            KYG+ G+YKTHLFG+PS+IVC P++CR VL +D  F  GY K+ + L   + +  V  A
Sbjct: 77  LKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMIDVSNA 136

Query: 135 EHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLSEMKTVAFKVLLH 194
           EHR  RRLI S I G++AL++Y+  +E IV+  LEE  +MK PVE L EMK V+FK ++H
Sbjct: 137 EHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFKAIVH 196

Query: 195 IFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELIRIFENVLKEKRA 254
           +F+G++    I K+   +TD + G  S  +++PG TF +AL+AR +L +I + V+ E+R 
Sbjct: 197 VFMGSSNQDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVVDERRL 256

Query: 255 NLKNKVTE-NRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMF 314
            ++N   E +++KD+ D+LLEV+DE+G+ L+D  I DLLIG  FAGHE++A S+M +I +
Sbjct: 257 MIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHESTATSLMWSITY 316

Query: 315 LSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLS 374
           L+++P  L KAK EQE+I + R   +  K L++KEIKQM YLS+VIDETLR  ++AF   
Sbjct: 317 LTQHPHILKKAKEEQEEITRTR--FSSQKQLSLKEIKQMVYLSQVIDETLRCANIAFATF 376

Query: 375 RETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPKRRAGSFIPFGA 434
           RE   DVN+NGY IPKGW++L+W RA+HMD E Y NP +F+PSRWD+   +AG+F+PFGA
Sbjct: 377 REATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDYNAKAGTFLPFGA 436

Query: 435 GMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDNCLARVIRDS 491
           G RLCPG DL+KLEI+IFLHYF+   YRLER+NP C    LP+ +P DNCLA+VI+ S
Sbjct: 437 GSRLCPGADLAKLEISIFLHYFLR-NYRLERINPECHVTSLPVSKPTDNCLAKVIKVS 491

BLAST of Lag0033026 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 4.2e-129
Identity = 228/470 (48.51%), Postives = 326/470 (69.36%), Query Frame = 0

Query: 21  ILRNLNQWRFVTRHG-GRYNLPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFASKYGKV 80
           ++R +N W + +  G  R+ LPPGD+GWP IG +L FL AF++  PDSF      +YG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 81  GMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNTVPKAEHRRLR 140
           G+YK H+FG+PS+IV   + CR VL +D+ F  G+  +T  L G K+   +   EH+RLR
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 141 RLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLSEMKTVAFKVLLHIFIGAN 200
           RL A+ ++G+EALS YI ++E  V+T L++W  M +  EFL+ ++ + F+++++IF+ + 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 201 TAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELIRIFENVLKEKRANLKNKV 260
           +   +D +E+ YT  + G  + AV++PG  + RALKAR  L+  F++++ E+R   K  +
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264

Query: 261 TENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPET 320
             N KKDM D LL V+DEDG+ LDD  IID+L+ +  AGHE+S H+IM A +FL E+PE 
Sbjct: 265 LSN-KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324

Query: 321 LLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETKVDV 380
           L +AKAEQE I+K+RP     KGL++KE ++ME+LS+V+DETLR  + + T  RE K DV
Sbjct: 325 LQRAKAEQEMILKSRPEG--QKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDV 384

Query: 381 NLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDN---PKRRAGSFIPFGAGMRL 440
            +NGY IPKGWK+L W R VH+DPE++ +P KFDP+RWDN   PK  AG+F+PFGAG  L
Sbjct: 385 EMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHL 444

Query: 441 CPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDNCLARV 487
           CPG DL+KLEI+IFLH+F+   Y+++R NP CP  YLP  RP DNCLAR+
Sbjct: 445 CPGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of Lag0033026 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 457.6 bits (1176), Expect = 1.8e-127
Identity = 229/487 (47.02%), Postives = 332/487 (68.17%), Query Frame = 0

Query: 3   VWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHG-GRYNLPPGDMGWPIIGTLLPFLHAFR 62
           +W  LI    G ++  + +L+ +N W +V++ G  ++ LPPGD+GWP+IG +  FL AF+
Sbjct: 9   MWFPLI--ILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 68

Query: 63  SGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRIL 122
           +  P+SFI  + ++YG+ G+YK H+FG P V+V  PE CR VL +D+ F  G+ K+T  L
Sbjct: 69  TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 128

Query: 123 TGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLS 182
            G K+   +   EH+RLRRL ++ ++G EALS+YI  +E  V T LE+W  M + +EFLS
Sbjct: 129 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 188

Query: 183 EMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELI 242
            ++ + FKV+++IF+ + +   +D +E+ YT+ + G  +  ++LPG  + RALKAR +L+
Sbjct: 189 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 248

Query: 243 RIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302
             F++++  +R   K  ++ NR KDM D L++V+DE+G+ LDD  IIDLL+ +  AGHE+
Sbjct: 249 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 308

Query: 303 SAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDET 362
           S H  M A + + E+P  L KAK EQE+IVK R   A  + LT+KE ++M YLS+VIDET
Sbjct: 309 SGHLTMWATILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDET 368

Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPK 422
           LR  + + T  RE K DV ++GY IPKGWK+L W R VH+DPEIY +P KFDPSRW+   
Sbjct: 369 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 428

Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDN 482
            +AG+F+PFG G  LCPG DL+KLEI+IFLH+F+   YR+ER NP CP  +LP  RP DN
Sbjct: 429 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486

Query: 483 CLARVIR 489
           CLAR+ R
Sbjct: 489 CLARITR 486

BLAST of Lag0033026 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 7.4e-126
Identity = 221/464 (47.63%), Postives = 311/464 (67.03%), Query Frame = 0

Query: 28  WRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFASKYGKVGMYKTHLF 87
           W      G R  LPPGDMGWP++G +  FL AF+SGRPDSFI  FA ++G+ G+Y+  +F
Sbjct: 33  WTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSGRPDSFIDSFARRFGRAGLYRAFMF 92

Query: 88  GSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNTVPKAEHRRLRRLIASLIS 147
            SP+++   PE C+ VLM+D+ FV G+ KAT  L G K+   +   EHRRLR+L A+ I+
Sbjct: 93  SSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRRLRKLTAAPIN 152

Query: 148 GNEALSIYIGHVEGIVVTCLEEW--RTMKKPVEFLSEMKTVAFKVLLHIFIGANTAAFID 207
           G +AL+ Y+G ++  VVT L  W  R      EFL+E++ + F++++ IF+G        
Sbjct: 153 GFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIFMGGADERTAA 212

Query: 208 KMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELIRIFENVLKEKRA-NLKNKVTENRK 267
           ++E+ YT+ + G  + A+DLPG  + +A++AR  L+   + VL E+RA   K        
Sbjct: 213 ELERTYTELNYGMRAMAIDLPGFAYHKAIRARRRLVAALQRVLDERRARGGKTAAGAAAP 272

Query: 268 KDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLLKAK 327
            DM D L+ V DE G+ L D  IID+L+ +  AGHE+S H  M A +FL ENPE L KAK
Sbjct: 273 VDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPEILAKAK 332

Query: 328 AEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETKVDVNLNGY 387
           AEQE I+++ P     KGLT+++ ++M YLS+V+DETLR  +++F   R+   DV +NGY
Sbjct: 333 AEQEAIMRSIPPG--QKGLTLRDFRKMAYLSQVVDETLRFVNISFVSFRQATRDVFVNGY 392

Query: 388 TIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSK 447
            IPKGWK+ +W R+VHMDP++Y +P KFDPSRW+ P  RAG+F+PFG G RLCPG DL+K
Sbjct: 393 LIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEGPPPRAGTFLPFGLGTRLCPGNDLAK 452

Query: 448 LEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDNCLARVIR 489
           LEI++FLH+F+  GY+L R NPNC   YLP PRP+DNCLA++ R
Sbjct: 453 LEISVFLHHFLL-GYKLTRKNPNCRVRYLPHPRPVDNCLAKITR 493

BLAST of Lag0033026 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 9.1e-124
Identity = 214/492 (43.50%), Postives = 324/492 (65.85%), Query Frame = 0

Query: 2   DVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGG--RYNLPPGDMGWPIIGTLLPFLHA 61
           D+W+ L  A     + V  ++R  + W  V   G   R  LPPG+MGWP++G++  FL A
Sbjct: 16  DLWV-LAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRA 75

Query: 62  FRSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR 121
           F+SG PD+FI  F  ++G+ G+Y+T +F SP+++   PE C+ VLM+DE FV G+ KAT 
Sbjct: 76  FKSGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATV 135

Query: 122 ILTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKK-PVE 181
            L G K+   +   +HRR+R+L A+ I+G +AL+ Y+  ++  VV  L  W + +   VE
Sbjct: 136 TLIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVE 195

Query: 182 FLSEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARN 241
           FL+E++ + FK+++ IF+     A ++ +E+ YTD + G  + A++LPG  + RAL+AR 
Sbjct: 196 FLTELRRMTFKIIVQIFMSGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARR 255

Query: 242 ELIRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAG 301
           +L+ + + VL  +RA        +   DM D L+E  DE G+ L D  I+D+LI +  AG
Sbjct: 256 KLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAG 315

Query: 302 HETSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVI 361
           HE+S H  M A +FL ENP+   +AKAEQE+I+++ P  A   GLT+++ K+M +LS+V+
Sbjct: 316 HESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIP--ATQNGLTLRDFKKMHFLSQVV 375

Query: 362 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWD 421
           DETLR  +++F   R+   D+ +NGY IPKGWK+ +W R+VHMD ++Y +P  F+PSRW+
Sbjct: 376 DETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWE 435

Query: 422 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRP 481
            P  +AG+F+PFG G RLCPG DL+KLEI++FLH+F+  GY+L+R NP C   YLP PRP
Sbjct: 436 GPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLL-GYKLKRANPKCRVRYLPHPRP 495

Query: 482 IDNCLARVIRDS 491
           +DNCLA + + S
Sbjct: 496 VDNCLATITKVS 503

BLAST of Lag0033026 vs. ExPASy TrEMBL
Match: A0A6J1K3T5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111490917 PE=3 SV=1)

HSP 1 Score: 936.4 bits (2419), Expect = 4.8e-269
Identity = 453/490 (92.45%), Postives = 472/490 (96.33%), Query Frame = 0

Query: 1   MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
           MD+WL LIGA AG Y+FVFGILRNLNQWRFVTRHG RYNLPPGDMGWP+IGTLLPFL AF
Sbjct: 3   MDIWLCLIGALAGVYVFVFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62

Query: 61  RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
           RSGRPDSFIHHF SKYGK GMYKTHLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63  RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122

Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
           LTGSKALN+VPK EHRRLRRLIASLISGNEALS+YIGHVEGIVVTCLEEW +MK+PVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 182

Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
           SEMKTVAFKVLLHIFIGAN+AAFIDKMEKLY DFHLGFMSTAVD+PGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 242

Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
           IRIFE VL+EKR NLKNKV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302

Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
           TSAH+IMRAIMFLSENPETLLKAKAEQE+IV ARPADAQHKGLTMKEIKQMEYLSKV+DE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEKIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362

Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
           TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENPH+FDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHQFDPSRWDNP 422

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLP P D
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482

Query: 481 NCLARVIRDS 491
           NCLARV+RDS
Sbjct: 483 NCLARVVRDS 492

BLAST of Lag0033026 vs. ExPASy TrEMBL
Match: A0A6J1ET68 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111437486 PE=3 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 1.8e-268
Identity = 452/490 (92.24%), Postives = 471/490 (96.12%), Query Frame = 0

Query: 1   MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
           MD+WL LIGA AG Y+F+FGILRNLNQWRFVTRHG RYNLPPGDMGWP+IGTLLPFL AF
Sbjct: 3   MDIWLCLIGALAGVYVFLFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62

Query: 61  RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
           RSGRPDSFIHHF SKYGK GMYKTHLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63  RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122

Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
           LTGSKALN+VPK EHRRLRRLIASLISGNEALS+YI HVEGIVVTCLEEW +MK+PVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIEHVEGIVVTCLEEWGSMKEPVEFL 182

Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
           SEMKTVAFKVLLHIFIGAN+AAFIDKMEKLY DFH+GFMSTAVD+PGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHMGFMSTAVDIPGTTFSRALKARNEL 242

Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
           IRIFE VL+EKR NLKNKV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302

Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
           TSAH+IMRAIMFLSENPETLLKAKAEQEQIV ARPADAQHKGLTMKEIKQMEYLSKV+DE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362

Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
           TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENPHKFDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 422

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLP P D
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482

Query: 481 NCLARVIRDS 491
           NCLARV+RDS
Sbjct: 483 NCLARVVRDS 492

BLAST of Lag0033026 vs. ExPASy TrEMBL
Match: A0A6J1GM12 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455188 PE=3 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.0e-267
Identity = 446/490 (91.02%), Postives = 472/490 (96.33%), Query Frame = 0

Query: 1   MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
           M+VW+ L+GA  GAYI VFGILRNLNQWRFVTRHGGRYNLPPGDMGWP++GTLLPFL AF
Sbjct: 1   MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60

Query: 61  RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
           RSGRPDSFIHHFASKYGK+GMYKT+LFGSPSVIVCVPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61  RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120

Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
           LTGSKALNTV + EHRR+RRLIASLISGN+ALSIYIGHVEGIV+TCLEEW TMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180

Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
           SEMKT+AFK+LLHIFIGANTAAFIDKMEKLYTDFHLGFMS+AVD+PGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
           IRIFE+VLKEKR NLKNKV ENRKKDMTDLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
           TSAHSIMRAIMFLSENPETL KAKAEQ+QIV ARP++ +H+GLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPSNERHRGLTMKEIKQMEYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
           TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHMDPEIYENP KFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPENYLPLPRP D
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480

Query: 481 NCLARVIRDS 491
           NCLARV+RDS
Sbjct: 481 NCLARVVRDS 490

BLAST of Lag0033026 vs. ExPASy TrEMBL
Match: A0A6J1I785 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470633 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 2.6e-267
Identity = 444/490 (90.61%), Postives = 471/490 (96.12%), Query Frame = 0

Query: 1   MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
           M+VW+ L+GA  GAY+FVFGILRNLNQWRFVTRHGGRYNLPPGDMGWP++GTLLPFL AF
Sbjct: 1   MEVWVWLVGALVGAYVFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60

Query: 61  RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
           RSGRPDSFIHHFAS+YGK+GMYKT+LFGSPSVIVCVPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61  RSGRPDSFIHHFASQYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120

Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
           LTGSKALNTV + EHRR+RRLIASLISGN+ALSIYIGHVEGIV+TCLEEW TMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180

Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
           SEMKT+AFK+LLHIFIGANTAAFIDKMEKLYTDFHLGFMS+AVD+PGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240

Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
           IRIFE+VLKEKR NLKNKV ENRKKDMTDLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300

Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
           TSAHSIMRAIMFLSENPETL KAKAEQE+IV ARP   +H+GLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLCKAKAEQEKIVNARPTSERHRGLTMKEIKQMEYLSKVIDE 360

Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
           TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHMDPEIYENP +FDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRTDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEEFDPSRWDNP 420

Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
           KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPENYLPLPRP D
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480

Query: 481 NCLARVIRDS 491
           NCLARV+RDS
Sbjct: 481 NCLARVVRDS 490

BLAST of Lag0033026 vs. ExPASy TrEMBL
Match: A0A5A7TAG0 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00740 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.0e-263
Identity = 449/488 (92.01%), Postives = 464/488 (95.08%), Query Frame = 0

Query: 3   VWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAFRS 62
           +WL L+GA   +YIFVF ILRNLNQW FVTRHG RYNLPPGDMGWP+IGTLLPFL AFRS
Sbjct: 4   LWLSLLGALVASYIFVFLILRNLNQWWFVTRHGRRYNLPPGDMGWPLIGTLLPFLQAFRS 63

Query: 63  GRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILT 122
           GRPDSFIH FA KYGK+GMYKT+LFGSPSVIV VPEVCRHVLMNDEQF FGYSKATRILT
Sbjct: 64  GRPDSFIHLFAYKYGKIGMYKTYLFGSPSVIVTVPEVCRHVLMNDEQFGFGYSKATRILT 123

Query: 123 GSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLSE 182
           GSKALNTVPK EHRRLRRLIASLISGNEALS+YIGHVEGIVVTCLEEW +MKKPVEFLSE
Sbjct: 124 GSKALNTVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWASMKKPVEFLSE 183

Query: 183 MKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELIR 242
           MKTVAFKVLLHIFIGANTAAFID+MEKLY DFHLGFMS+ VDLPGTTFSRALKARNELI+
Sbjct: 184 MKTVAFKVLLHIFIGANTAAFIDRMEKLYNDFHLGFMSSPVDLPGTTFSRALKARNELIK 243

Query: 243 IFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETS 302
           IFENVLKEKR NLK KV ENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETS
Sbjct: 244 IFENVLKEKRENLKKKVAENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETS 303

Query: 303 AHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDETL 362
           AHSIMRAIMFLSENPETL KAKAEQEQIVKARPADAQHKGLTMKEIKQMEYL KVIDETL
Sbjct: 304 AHSIMRAIMFLSENPETLQKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLFKVIDETL 363

Query: 363 RKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPKR 422
           RKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENP KFDPSRW+NPKR
Sbjct: 364 RKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWENPKR 423

Query: 423 RAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDNC 482
           R GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLPRP DNC
Sbjct: 424 RGGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDNC 483

Query: 483 LARVIRDS 491
           LARV+RDS
Sbjct: 484 LARVVRDS 491

BLAST of Lag0033026 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 463.0 bits (1190), Expect = 3.0e-130
Identity = 228/470 (48.51%), Postives = 326/470 (69.36%), Query Frame = 0

Query: 21  ILRNLNQWRFVTRHG-GRYNLPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFASKYGKV 80
           ++R +N W + +  G  R+ LPPGD+GWP IG +L FL AF++  PDSF      +YG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 81  GMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNTVPKAEHRRLR 140
           G+YK H+FG+PS+IV   + CR VL +D+ F  G+  +T  L G K+   +   EH+RLR
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 141 RLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLSEMKTVAFKVLLHIFIGAN 200
           RL A+ ++G+EALS YI ++E  V+T L++W  M +  EFL+ ++ + F+++++IF+ + 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 201 TAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELIRIFENVLKEKRANLKNKV 260
           +   +D +E+ YT  + G  + AV++PG  + RALKAR  L+  F++++ E+R   K  +
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264

Query: 261 TENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPET 320
             N KKDM D LL V+DEDG+ LDD  IID+L+ +  AGHE+S H+IM A +FL E+PE 
Sbjct: 265 LSN-KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324

Query: 321 LLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETKVDV 380
           L +AKAEQE I+K+RP     KGL++KE ++ME+LS+V+DETLR  + + T  RE K DV
Sbjct: 325 LQRAKAEQEMILKSRPEG--QKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDV 384

Query: 381 NLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDN---PKRRAGSFIPFGAGMRL 440
            +NGY IPKGWK+L W R VH+DPE++ +P KFDP+RWDN   PK  AG+F+PFGAG  L
Sbjct: 385 EMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHL 444

Query: 441 CPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDNCLARV 487
           CPG DL+KLEI+IFLH+F+   Y+++R NP CP  YLP  RP DNCLAR+
Sbjct: 445 CPGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of Lag0033026 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 457.6 bits (1176), Expect = 1.3e-128
Identity = 229/487 (47.02%), Postives = 332/487 (68.17%), Query Frame = 0

Query: 3   VWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHG-GRYNLPPGDMGWPIIGTLLPFLHAFR 62
           +W  LI    G ++  + +L+ +N W +V++ G  ++ LPPGD+GWP+IG +  FL AF+
Sbjct: 9   MWFPLI--ILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 68

Query: 63  SGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRIL 122
           +  P+SFI  + ++YG+ G+YK H+FG P V+V  PE CR VL +D+ F  G+ K+T  L
Sbjct: 69  TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 128

Query: 123 TGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLS 182
            G K+   +   EH+RLRRL ++ ++G EALS+YI  +E  V T LE+W  M + +EFLS
Sbjct: 129 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 188

Query: 183 EMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELI 242
            ++ + FKV+++IF+ + +   +D +E+ YT+ + G  +  ++LPG  + RALKAR +L+
Sbjct: 189 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 248

Query: 243 RIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302
             F++++  +R   K  ++ NR KDM D L++V+DE+G+ LDD  IIDLL+ +  AGHE+
Sbjct: 249 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 308

Query: 303 SAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDET 362
           S H  M A + + E+P  L KAK EQE+IVK R   A  + LT+KE ++M YLS+VIDET
Sbjct: 309 SGHLTMWATILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDET 368

Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPK 422
           LR  + + T  RE K DV ++GY IPKGWK+L W R VH+DPEIY +P KFDPSRW+   
Sbjct: 369 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 428

Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDN 482
            +AG+F+PFG G  LCPG DL+KLEI+IFLH+F+   YR+ER NP CP  +LP  RP DN
Sbjct: 429 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486

Query: 483 CLARVIR 489
           CLAR+ R
Sbjct: 489 CLARITR 486

BLAST of Lag0033026 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 457.6 bits (1176), Expect = 1.3e-128
Identity = 229/487 (47.02%), Postives = 332/487 (68.17%), Query Frame = 0

Query: 3   VWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHG-GRYNLPPGDMGWPIIGTLLPFLHAFR 62
           +W  LI    G ++  + +L+ +N W +V++ G  ++ LPPGD+GWP+IG +  FL AF+
Sbjct: 9   MWFPLI--ILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 68

Query: 63  SGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRIL 122
           +  P+SFI  + ++YG+ G+YK H+FG P V+V  PE CR VL +D+ F  G+ K+T  L
Sbjct: 69  TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 128

Query: 123 TGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLS 182
            G K+   +   EH+RLRRL ++ ++G EALS+YI  +E  V T LE+W  M + +EFLS
Sbjct: 129 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 188

Query: 183 EMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELI 242
            ++ + FKV+++IF+ + +   +D +E+ YT+ + G  +  ++LPG  + RALKAR +L+
Sbjct: 189 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 248

Query: 243 RIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302
             F++++  +R   K  ++ NR KDM D L++V+DE+G+ LDD  IIDLL+ +  AGHE+
Sbjct: 249 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 308

Query: 303 SAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDET 362
           S H  M A + + E+P  L KAK EQE+IVK R   A  + LT+KE ++M YLS+VIDET
Sbjct: 309 SGHLTMWATILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDET 368

Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPK 422
           LR  + + T  RE K DV ++GY IPKGWK+L W R VH+DPEIY +P KFDPSRW+   
Sbjct: 369 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 428

Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDN 482
            +AG+F+PFG G  LCPG DL+KLEI+IFLH+F+   YR+ER NP CP  +LP  RP DN
Sbjct: 429 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486

Query: 483 CLARVIR 489
           CLAR+ R
Sbjct: 489 CLARITR 486

BLAST of Lag0033026 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 231.9 bits (590), Expect = 1.1e-60
Identity = 134/424 (31.60%), Postives = 225/424 (53.07%), Query Frame = 0

Query: 34  HGGRYNLPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVI 93
           HG ++  P G +GWP+IG  + F+ +  S RP+SF+      YG+V  +K+H+FG+ +++
Sbjct: 47  HGPKF--PHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRV--FKSHIFGTATIV 106

Query: 94  VCVPEVCRHVLMNDE-QFVFGYSKATRILTGSKALNTVPKAEHRRLRRLIASLISGNEAL 153
               EV R VL +D   FV  Y K  R L G  ++  +  + HRR   L+ S +      
Sbjct: 107 STDAEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLK 166

Query: 154 SIYIGHVEGIVVTCLEEWRTMKKPVEFLSEMKTVAFKVLLHIFIGANTAAFIDKMEKLYT 213
           +  +  +   +   ++ W +  +PV      KTVAFKVL    I       ++++++ + 
Sbjct: 167 AQIVRDMHKFLSESMDLW-SEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFE 226

Query: 214 DFHLGFMSTAVDLPGTTFSRALKARNELIRIFENVLKEKRANLKNKVTEN-RKKDMTDLL 273
           +F  G MS  ++ PGT   R+L+A+  +++  E +++ K    KNK  ++   KD+ D+L
Sbjct: 227 NFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVL 286

Query: 274 LEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLLKAKAEQEQIV 333
           L+   +  + L    I + +I     GH++    I  A+ FLS++P  L     E  ++ 
Sbjct: 287 LK---DSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLK 346

Query: 334 KARPADAQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWK 393
             +  +   + L   +   + +  KVI ETLR  ++   + R+   DV + GY IPKGW 
Sbjct: 347 SLK--ELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWC 406

Query: 394 ILVWTRAVHMDPEIYENPHKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFL 453
            L + R+VH+D   YE+P+KF+P RW        SF PFG G RLCPG+DL++LE ++FL
Sbjct: 407 FLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFL 460

Query: 454 HYFI 456
           H+ +
Sbjct: 467 HHLV 460

BLAST of Lag0033026 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 219.5 bits (558), Expect = 5.8e-57
Identity = 134/421 (31.83%), Postives = 219/421 (52.02%), Query Frame = 0

Query: 40  LPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEV 99
           +P G +GWP+IG  L F+    S RP +F+    S YGKV  +KT++ G+P +I    EV
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKV--FKTNIIGTPIIISTDAEV 128

Query: 100 CRHVLMN-DEQFVFGYSKATRILTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGH 159
            + VL N    FV  Y K+   L G  ++ ++     +RL  LI + +            
Sbjct: 129 NKVVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRD 188

Query: 160 VEGIVVTCLEEWRTMKKPVEFLSEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGF 219
           +E  VV  L  W  +   V    E+K + F++L+ + +  +    ++ ++  + +F  G 
Sbjct: 189 IEASVVLTLASWAQLPL-VHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGL 248

Query: 220 MSTAVDLPGTTFSRALKARNELIRIFENVLKEKRANLKNKVTENRKKDMTDLLL-EVRDE 279
           +   +  PGT   ++LKA+  LI++ + V++E++  +    T +   D+ D+LL +  D 
Sbjct: 249 ICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAM---TTTSPANDVVDVLLRDGGDS 308

Query: 280 DGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPAD 339
           + Q      +   ++     G ET   ++  A+ FLS+NP  L K   E+   +K R  +
Sbjct: 309 EKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKL-VEENMEMKRRKLE 368

Query: 340 AQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTR 399
              +     +   + +   VI+ETLR  ++   + R+   DV + GY IPKGW +L    
Sbjct: 369 LGEE-YKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFI 428

Query: 400 AVHMDPEIYENPHKFDPSRWDNPKRRAGS---FIPFGAGMRLCPGIDLSKLEIAIFLHYF 456
           +VHMD +IY+NP++FDP RWD     A S   F PFG G RLCPG++LSKLEI+IFLH+ 
Sbjct: 429 SVHMDEDIYDNPYQFDPWRWDRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHL 481

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022995340.19.9e-26992.45beta-amyrin 11-oxidase-like [Cucurbita maxima][more]
KAG7036178.12.9e-26892.04Beta-amyrin 11-oxidase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022931256.13.8e-26892.24beta-amyrin 11-oxidase-like [Cucurbita moschata][more]
XP_023554757.11.1e-26791.43beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo][more]
XP_022952525.14.2e-26791.02beta-amyrin 11-oxidase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
B5BSX11.8e-14051.67Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1[more]
O230514.2e-12948.51Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1[more]
Q9C5Y21.8e-12747.02Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2[more]
Q9AXH97.4e-12647.63Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1[more]
Q5VRM79.1e-12443.50Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1K3T54.8e-26992.45beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111490917 PE=3 SV=... [more]
A0A6J1ET681.8e-26892.24beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111437486 PE=3 S... [more]
A0A6J1GM122.0e-26791.02beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455188 PE=3 S... [more]
A0A6J1I7852.6e-26790.61beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470633 PE=3 SV=... [more]
A0A5A7TAG01.0e-26392.01Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
Match NameE-valueIdentityDescription
AT1G05160.13.0e-13048.51cytochrome P450, family 88, subfamily A, polypeptide 3 [more]
AT2G32440.11.3e-12847.02ent-kaurenoic acid hydroxylase 2 [more]
AT2G32440.21.3e-12847.02ent-kaurenoic acid hydroxylase 2 [more]
AT3G13730.11.1e-6031.60cytochrome P450, family 90, subfamily D, polypeptide 1 [more]
AT4G36380.15.8e-5731.83Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 427..437
score: 56.24
coord: 306..332
score: 21.44
coord: 286..303
score: 28.65
coord: 437..460
score: 22.64
coord: 395..419
score: 27.59
IPR001128Cytochrome P450PRINTSPR00385P450coord: 297..314
score: 29.48
coord: 437..448
score: 31.75
coord: 428..437
score: 57.97
coord: 356..367
score: 36.49
IPR001128Cytochrome P450PFAMPF00067p450coord: 41..455
e-value: 2.4E-68
score: 231.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 32..487
e-value: 1.5E-103
score: 349.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 37..487
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 19..467
NoneNo IPR availablePANTHERPTHR24286:SF199CYTOCHROME P450 88D6coord: 19..467
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 430..439

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033026.1Lag0033026.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen