Homology
BLAST of Lag0033026 vs. NCBI nr
Match:
XP_022995340.1 (beta-amyrin 11-oxidase-like [Cucurbita maxima])
HSP 1 Score: 936.4 bits (2419), Expect = 9.9e-269
Identity = 453/490 (92.45%), Postives = 472/490 (96.33%), Query Frame = 0
Query: 1 MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
MD+WL LIGA AG Y+FVFGILRNLNQWRFVTRHG RYNLPPGDMGWP+IGTLLPFL AF
Sbjct: 3 MDIWLCLIGALAGVYVFVFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62
Query: 61 RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHF SKYGK GMYKTHLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63 RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122
Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
LTGSKALN+VPK EHRRLRRLIASLISGNEALS+YIGHVEGIVVTCLEEW +MK+PVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 182
Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
SEMKTVAFKVLLHIFIGAN+AAFIDKMEKLY DFHLGFMSTAVD+PGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 242
Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
IRIFE VL+EKR NLKNKV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302
Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
TSAH+IMRAIMFLSENPETLLKAKAEQE+IV ARPADAQHKGLTMKEIKQMEYLSKV+DE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEKIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENPH+FDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHQFDPSRWDNP 422
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLP P D
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482
Query: 481 NCLARVIRDS 491
NCLARV+RDS
Sbjct: 483 NCLARVVRDS 492
BLAST of Lag0033026 vs. NCBI nr
Match:
KAG7036178.1 (Beta-amyrin 11-oxidase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 934.9 bits (2415), Expect = 2.9e-268
Identity = 451/490 (92.04%), Postives = 471/490 (96.12%), Query Frame = 0
Query: 1 MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
MD+W+ LIGA AG Y+F+FGI RNLNQWRFVTRHG RYNLPPGDMGWP+IGTLLPFL AF
Sbjct: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
Query: 61 RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHF SKYGK GMY+THLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
LTGSKALN+VPK EHRRLRRLIASLISGNEALS+YIGHVEGIVVTCLEEW +MK+PVEFL
Sbjct: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
SEMKTVAFKVLLHIFIGAN+AAFIDKMEKLY DFHLGFMSTAVD+PGTTFSRALKARNEL
Sbjct: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
IRIFE VL+EKR NLKNKV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
TSAH+IMRAIMFLSENPETLLKAKAEQEQIV ARPADAQHKGLTMKEIKQMEYLSKV+DE
Sbjct: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENPHKFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLP P D
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
Query: 481 NCLARVIRDS 491
NCLARV+RDS
Sbjct: 481 NCLARVVRDS 490
BLAST of Lag0033026 vs. NCBI nr
Match:
XP_022931256.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])
HSP 1 Score: 934.5 bits (2414), Expect = 3.8e-268
Identity = 452/490 (92.24%), Postives = 471/490 (96.12%), Query Frame = 0
Query: 1 MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
MD+WL LIGA AG Y+F+FGILRNLNQWRFVTRHG RYNLPPGDMGWP+IGTLLPFL AF
Sbjct: 3 MDIWLCLIGALAGVYVFLFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62
Query: 61 RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHF SKYGK GMYKTHLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63 RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122
Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
LTGSKALN+VPK EHRRLRRLIASLISGNEALS+YI HVEGIVVTCLEEW +MK+PVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIEHVEGIVVTCLEEWGSMKEPVEFL 182
Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
SEMKTVAFKVLLHIFIGAN+AAFIDKMEKLY DFH+GFMSTAVD+PGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHMGFMSTAVDIPGTTFSRALKARNEL 242
Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
IRIFE VL+EKR NLKNKV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302
Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
TSAH+IMRAIMFLSENPETLLKAKAEQEQIV ARPADAQHKGLTMKEIKQMEYLSKV+DE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENPHKFDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 422
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLP P D
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482
Query: 481 NCLARVIRDS 491
NCLARV+RDS
Sbjct: 483 NCLARVVRDS 492
BLAST of Lag0033026 vs. NCBI nr
Match:
XP_023554757.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 932.9 bits (2410), Expect = 1.1e-267
Identity = 448/490 (91.43%), Postives = 472/490 (96.33%), Query Frame = 0
Query: 1 MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
M+VW+ L+GA GAYIFVF ILRNLNQWRFVTRHGGRYNLPPGDMGWP++GTLLPFL AF
Sbjct: 1 MEVWVWLVGALVGAYIFVFVILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
Query: 61 RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHFASKYGK+GMYKT+LFGSPSVIVCVPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61 RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
LTGSKALNTV + EHRR+RRLIASLISGN+ALSIYIGHVEGIV+TCLEEW TMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
SEMKT+AFK+LLHIFIGANTAAFIDKMEKLYTDFHLGFMS+AVD+PGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
IRIFE+VLKEKR NLKNKV ENRKKDMTDLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
TSAHSIMRAIMFLSENPETL KAKAEQEQIV ARPA+ +H+GLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQEQIVNARPANERHRGLTMKEIKQMEYLSKVIDE 360
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHMDPEIYENP KFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPENYLPLPRP D
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
Query: 481 NCLARVIRDS 491
NCLARV+RDS
Sbjct: 481 NCLARVVRDS 490
BLAST of Lag0033026 vs. NCBI nr
Match:
XP_022952525.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])
HSP 1 Score: 931.0 bits (2405), Expect = 4.2e-267
Identity = 446/490 (91.02%), Postives = 472/490 (96.33%), Query Frame = 0
Query: 1 MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
M+VW+ L+GA GAYI VFGILRNLNQWRFVTRHGGRYNLPPGDMGWP++GTLLPFL AF
Sbjct: 1 MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
Query: 61 RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHFASKYGK+GMYKT+LFGSPSVIVCVPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61 RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
LTGSKALNTV + EHRR+RRLIASLISGN+ALSIYIGHVEGIV+TCLEEW TMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
SEMKT+AFK+LLHIFIGANTAAFIDKMEKLYTDFHLGFMS+AVD+PGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
IRIFE+VLKEKR NLKNKV ENRKKDMTDLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
TSAHSIMRAIMFLSENPETL KAKAEQ+QIV ARP++ +H+GLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPSNERHRGLTMKEIKQMEYLSKVIDE 360
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHMDPEIYENP KFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPENYLPLPRP D
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
Query: 481 NCLARVIRDS 491
NCLARV+RDS
Sbjct: 481 NCLARVVRDS 490
BLAST of Lag0033026 vs. ExPASy Swiss-Prot
Match:
B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)
HSP 1 Score: 500.7 bits (1288), Expect = 1.8e-140
Identity = 247/478 (51.67%), Postives = 339/478 (70.92%), Query Frame = 0
Query: 15 YIFVFGILRNLNQWRF-VTRHGGRYNLPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFA 74
YIF +RNLN W + V + LPPGDMGWP+IG LL F+ F SG PDSFI++
Sbjct: 17 YIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFINNLV 76
Query: 75 SKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNTVPKA 134
KYG+ G+YKTHLFG+PS+IVC P++CR VL +D F GY K+ + L + + V A
Sbjct: 77 LKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMIDVSNA 136
Query: 135 EHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLSEMKTVAFKVLLH 194
EHR RRLI S I G++AL++Y+ +E IV+ LEE +MK PVE L EMK V+FK ++H
Sbjct: 137 EHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFKAIVH 196
Query: 195 IFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELIRIFENVLKEKRA 254
+F+G++ I K+ +TD + G S +++PG TF +AL+AR +L +I + V+ E+R
Sbjct: 197 VFMGSSNQDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVVDERRL 256
Query: 255 NLKNKVTE-NRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMF 314
++N E +++KD+ D+LLEV+DE+G+ L+D I DLLIG FAGHE++A S+M +I +
Sbjct: 257 MIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHESTATSLMWSITY 316
Query: 315 LSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLS 374
L+++P L KAK EQE+I + R + K L++KEIKQM YLS+VIDETLR ++AF
Sbjct: 317 LTQHPHILKKAKEEQEEITRTR--FSSQKQLSLKEIKQMVYLSQVIDETLRCANIAFATF 376
Query: 375 RETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPKRRAGSFIPFGA 434
RE DVN+NGY IPKGW++L+W RA+HMD E Y NP +F+PSRWD+ +AG+F+PFGA
Sbjct: 377 REATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDYNAKAGTFLPFGA 436
Query: 435 GMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDNCLARVIRDS 491
G RLCPG DL+KLEI+IFLHYF+ YRLER+NP C LP+ +P DNCLA+VI+ S
Sbjct: 437 GSRLCPGADLAKLEISIFLHYFLR-NYRLERINPECHVTSLPVSKPTDNCLAKVIKVS 491
BLAST of Lag0033026 vs. ExPASy Swiss-Prot
Match:
O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 463.0 bits (1190), Expect = 4.2e-129
Identity = 228/470 (48.51%), Postives = 326/470 (69.36%), Query Frame = 0
Query: 21 ILRNLNQWRFVTRHG-GRYNLPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFASKYGKV 80
++R +N W + + G R+ LPPGD+GWP IG +L FL AF++ PDSF +YG
Sbjct: 25 LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84
Query: 81 GMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNTVPKAEHRRLR 140
G+YK H+FG+PS+IV + CR VL +D+ F G+ +T L G K+ + EH+RLR
Sbjct: 85 GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144
Query: 141 RLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLSEMKTVAFKVLLHIFIGAN 200
RL A+ ++G+EALS YI ++E V+T L++W M + EFL+ ++ + F+++++IF+ +
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204
Query: 201 TAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELIRIFENVLKEKRANLKNKV 260
+ +D +E+ YT + G + AV++PG + RALKAR L+ F++++ E+R K +
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264
Query: 261 TENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPET 320
N KKDM D LL V+DEDG+ LDD IID+L+ + AGHE+S H+IM A +FL E+PE
Sbjct: 265 LSN-KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324
Query: 321 LLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETKVDV 380
L +AKAEQE I+K+RP KGL++KE ++ME+LS+V+DETLR + + T RE K DV
Sbjct: 325 LQRAKAEQEMILKSRPEG--QKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDV 384
Query: 381 NLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDN---PKRRAGSFIPFGAGMRL 440
+NGY IPKGWK+L W R VH+DPE++ +P KFDP+RWDN PK AG+F+PFGAG L
Sbjct: 385 EMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHL 444
Query: 441 CPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDNCLARV 487
CPG DL+KLEI+IFLH+F+ Y+++R NP CP YLP RP DNCLAR+
Sbjct: 445 CPGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487
BLAST of Lag0033026 vs. ExPASy Swiss-Prot
Match:
Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)
HSP 1 Score: 457.6 bits (1176), Expect = 1.8e-127
Identity = 229/487 (47.02%), Postives = 332/487 (68.17%), Query Frame = 0
Query: 3 VWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHG-GRYNLPPGDMGWPIIGTLLPFLHAFR 62
+W LI G ++ + +L+ +N W +V++ G ++ LPPGD+GWP+IG + FL AF+
Sbjct: 9 MWFPLI--ILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 68
Query: 63 SGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRIL 122
+ P+SFI + ++YG+ G+YK H+FG P V+V PE CR VL +D+ F G+ K+T L
Sbjct: 69 TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 128
Query: 123 TGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLS 182
G K+ + EH+RLRRL ++ ++G EALS+YI +E V T LE+W M + +EFLS
Sbjct: 129 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 188
Query: 183 EMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELI 242
++ + FKV+++IF+ + + +D +E+ YT+ + G + ++LPG + RALKAR +L+
Sbjct: 189 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 248
Query: 243 RIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302
F++++ +R K ++ NR KDM D L++V+DE+G+ LDD IIDLL+ + AGHE+
Sbjct: 249 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 308
Query: 303 SAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDET 362
S H M A + + E+P L KAK EQE+IVK R A + LT+KE ++M YLS+VIDET
Sbjct: 309 SGHLTMWATILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDET 368
Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPK 422
LR + + T RE K DV ++GY IPKGWK+L W R VH+DPEIY +P KFDPSRW+
Sbjct: 369 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 428
Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDN 482
+AG+F+PFG G LCPG DL+KLEI+IFLH+F+ YR+ER NP CP +LP RP DN
Sbjct: 429 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486
Query: 483 CLARVIR 489
CLAR+ R
Sbjct: 489 CLARITR 486
BLAST of Lag0033026 vs. ExPASy Swiss-Prot
Match:
Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 452.2 bits (1162), Expect = 7.4e-126
Identity = 221/464 (47.63%), Postives = 311/464 (67.03%), Query Frame = 0
Query: 28 WRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFASKYGKVGMYKTHLF 87
W G R LPPGDMGWP++G + FL AF+SGRPDSFI FA ++G+ G+Y+ +F
Sbjct: 33 WTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSGRPDSFIDSFARRFGRAGLYRAFMF 92
Query: 88 GSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNTVPKAEHRRLRRLIASLIS 147
SP+++ PE C+ VLM+D+ FV G+ KAT L G K+ + EHRRLR+L A+ I+
Sbjct: 93 SSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRRLRKLTAAPIN 152
Query: 148 GNEALSIYIGHVEGIVVTCLEEW--RTMKKPVEFLSEMKTVAFKVLLHIFIGANTAAFID 207
G +AL+ Y+G ++ VVT L W R EFL+E++ + F++++ IF+G
Sbjct: 153 GFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIFMGGADERTAA 212
Query: 208 KMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELIRIFENVLKEKRA-NLKNKVTENRK 267
++E+ YT+ + G + A+DLPG + +A++AR L+ + VL E+RA K
Sbjct: 213 ELERTYTELNYGMRAMAIDLPGFAYHKAIRARRRLVAALQRVLDERRARGGKTAAGAAAP 272
Query: 268 KDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLLKAK 327
DM D L+ V DE G+ L D IID+L+ + AGHE+S H M A +FL ENPE L KAK
Sbjct: 273 VDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPEILAKAK 332
Query: 328 AEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETKVDVNLNGY 387
AEQE I+++ P KGLT+++ ++M YLS+V+DETLR +++F R+ DV +NGY
Sbjct: 333 AEQEAIMRSIPPG--QKGLTLRDFRKMAYLSQVVDETLRFVNISFVSFRQATRDVFVNGY 392
Query: 388 TIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSK 447
IPKGWK+ +W R+VHMDP++Y +P KFDPSRW+ P RAG+F+PFG G RLCPG DL+K
Sbjct: 393 LIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEGPPPRAGTFLPFGLGTRLCPGNDLAK 452
Query: 448 LEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDNCLARVIR 489
LEI++FLH+F+ GY+L R NPNC YLP PRP+DNCLA++ R
Sbjct: 453 LEISVFLHHFLL-GYKLTRKNPNCRVRYLPHPRPVDNCLAKITR 493
BLAST of Lag0033026 vs. ExPASy Swiss-Prot
Match:
Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)
HSP 1 Score: 445.3 bits (1144), Expect = 9.1e-124
Identity = 214/492 (43.50%), Postives = 324/492 (65.85%), Query Frame = 0
Query: 2 DVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGG--RYNLPPGDMGWPIIGTLLPFLHA 61
D+W+ L A + V ++R + W V G R LPPG+MGWP++G++ FL A
Sbjct: 16 DLWV-LAAAVVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRA 75
Query: 62 FRSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATR 121
F+SG PD+FI F ++G+ G+Y+T +F SP+++ PE C+ VLM+DE FV G+ KAT
Sbjct: 76 FKSGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATV 135
Query: 122 ILTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKK-PVE 181
L G K+ + +HRR+R+L A+ I+G +AL+ Y+ ++ VV L W + + VE
Sbjct: 136 TLIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVE 195
Query: 182 FLSEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARN 241
FL+E++ + FK+++ IF+ A ++ +E+ YTD + G + A++LPG + RAL+AR
Sbjct: 196 FLTELRRMTFKIIVQIFMSGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARR 255
Query: 242 ELIRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAG 301
+L+ + + VL +RA + DM D L+E DE G+ L D I+D+LI + AG
Sbjct: 256 KLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAG 315
Query: 302 HETSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVI 361
HE+S H M A +FL ENP+ +AKAEQE+I+++ P A GLT+++ K+M +LS+V+
Sbjct: 316 HESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIP--ATQNGLTLRDFKKMHFLSQVV 375
Query: 362 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWD 421
DETLR +++F R+ D+ +NGY IPKGWK+ +W R+VHMD ++Y +P F+PSRW+
Sbjct: 376 DETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWE 435
Query: 422 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRP 481
P +AG+F+PFG G RLCPG DL+KLEI++FLH+F+ GY+L+R NP C YLP PRP
Sbjct: 436 GPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLL-GYKLKRANPKCRVRYLPHPRP 495
Query: 482 IDNCLARVIRDS 491
+DNCLA + + S
Sbjct: 496 VDNCLATITKVS 503
BLAST of Lag0033026 vs. ExPASy TrEMBL
Match:
A0A6J1K3T5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111490917 PE=3 SV=1)
HSP 1 Score: 936.4 bits (2419), Expect = 4.8e-269
Identity = 453/490 (92.45%), Postives = 472/490 (96.33%), Query Frame = 0
Query: 1 MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
MD+WL LIGA AG Y+FVFGILRNLNQWRFVTRHG RYNLPPGDMGWP+IGTLLPFL AF
Sbjct: 3 MDIWLCLIGALAGVYVFVFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62
Query: 61 RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHF SKYGK GMYKTHLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63 RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122
Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
LTGSKALN+VPK EHRRLRRLIASLISGNEALS+YIGHVEGIVVTCLEEW +MK+PVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 182
Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
SEMKTVAFKVLLHIFIGAN+AAFIDKMEKLY DFHLGFMSTAVD+PGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 242
Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
IRIFE VL+EKR NLKNKV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302
Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
TSAH+IMRAIMFLSENPETLLKAKAEQE+IV ARPADAQHKGLTMKEIKQMEYLSKV+DE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEKIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENPH+FDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHQFDPSRWDNP 422
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLP P D
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482
Query: 481 NCLARVIRDS 491
NCLARV+RDS
Sbjct: 483 NCLARVVRDS 492
BLAST of Lag0033026 vs. ExPASy TrEMBL
Match:
A0A6J1ET68 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111437486 PE=3 SV=1)
HSP 1 Score: 934.5 bits (2414), Expect = 1.8e-268
Identity = 452/490 (92.24%), Postives = 471/490 (96.12%), Query Frame = 0
Query: 1 MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
MD+WL LIGA AG Y+F+FGILRNLNQWRFVTRHG RYNLPPGDMGWP+IGTLLPFL AF
Sbjct: 3 MDIWLCLIGALAGVYVFLFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 62
Query: 61 RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHF SKYGK GMYKTHLFGSPSVIV VPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 63 RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122
Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
LTGSKALN+VPK EHRRLRRLIASLISGNEALS+YI HVEGIVVTCLEEW +MK+PVEFL
Sbjct: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIEHVEGIVVTCLEEWGSMKEPVEFL 182
Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
SEMKTVAFKVLLHIFIGAN+AAFIDKMEKLY DFH+GFMSTAVD+PGTTFSRALKARNEL
Sbjct: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHMGFMSTAVDIPGTTFSRALKARNEL 242
Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
IRIFE VL+EKR NLKNKV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHE
Sbjct: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302
Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
TSAH+IMRAIMFLSENPETLLKAKAEQEQIV ARPADAQHKGLTMKEIKQMEYLSKV+DE
Sbjct: 303 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENPHKFDPSRWDNP
Sbjct: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 422
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLP P D
Sbjct: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482
Query: 481 NCLARVIRDS 491
NCLARV+RDS
Sbjct: 483 NCLARVVRDS 492
BLAST of Lag0033026 vs. ExPASy TrEMBL
Match:
A0A6J1GM12 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455188 PE=3 SV=1)
HSP 1 Score: 931.0 bits (2405), Expect = 2.0e-267
Identity = 446/490 (91.02%), Postives = 472/490 (96.33%), Query Frame = 0
Query: 1 MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
M+VW+ L+GA GAYI VFGILRNLNQWRFVTRHGGRYNLPPGDMGWP++GTLLPFL AF
Sbjct: 1 MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
Query: 61 RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHFASKYGK+GMYKT+LFGSPSVIVCVPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61 RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
LTGSKALNTV + EHRR+RRLIASLISGN+ALSIYIGHVEGIV+TCLEEW TMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
SEMKT+AFK+LLHIFIGANTAAFIDKMEKLYTDFHLGFMS+AVD+PGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
IRIFE+VLKEKR NLKNKV ENRKKDMTDLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
TSAHSIMRAIMFLSENPETL KAKAEQ+QIV ARP++ +H+GLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPSNERHRGLTMKEIKQMEYLSKVIDE 360
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHMDPEIYENP KFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPENYLPLPRP D
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
Query: 481 NCLARVIRDS 491
NCLARV+RDS
Sbjct: 481 NCLARVVRDS 490
BLAST of Lag0033026 vs. ExPASy TrEMBL
Match:
A0A6J1I785 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470633 PE=3 SV=1)
HSP 1 Score: 930.6 bits (2404), Expect = 2.6e-267
Identity = 444/490 (90.61%), Postives = 471/490 (96.12%), Query Frame = 0
Query: 1 MDVWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAF 60
M+VW+ L+GA GAY+FVFGILRNLNQWRFVTRHGGRYNLPPGDMGWP++GTLLPFL AF
Sbjct: 1 MEVWVWLVGALVGAYVFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
Query: 61 RSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRI 120
RSGRPDSFIHHFAS+YGK+GMYKT+LFGSPSVIVCVPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61 RSGRPDSFIHHFASQYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
Query: 121 LTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFL 180
LTGSKALNTV + EHRR+RRLIASLISGN+ALSIYIGHVEGIV+TCLEEW TMKKPVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
Query: 181 SEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNEL 240
SEMKT+AFK+LLHIFIGANTAAFIDKMEKLYTDFHLGFMS+AVD+PGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
Query: 241 IRIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHE 300
IRIFE+VLKEKR NLKNKV ENRKKDMTDLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
Query: 301 TSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDE 360
TSAHSIMRAIMFLSENPETL KAKAEQE+IV ARP +H+GLTMKEIKQMEYLSKVIDE
Sbjct: 301 TSAHSIMRAIMFLSENPETLCKAKAEQEKIVNARPTSERHRGLTMKEIKQMEYLSKVIDE 360
Query: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNP 420
TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHMDPEIYENP +FDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRTDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEEFDPSRWDNP 420
Query: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPID 480
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPENYLPLPRP D
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
Query: 481 NCLARVIRDS 491
NCLARV+RDS
Sbjct: 481 NCLARVVRDS 490
BLAST of Lag0033026 vs. ExPASy TrEMBL
Match:
A0A5A7TAG0 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00740 PE=3 SV=1)
HSP 1 Score: 918.7 bits (2373), Expect = 1.0e-263
Identity = 449/488 (92.01%), Postives = 464/488 (95.08%), Query Frame = 0
Query: 3 VWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPIIGTLLPFLHAFRS 62
+WL L+GA +YIFVF ILRNLNQW FVTRHG RYNLPPGDMGWP+IGTLLPFL AFRS
Sbjct: 4 LWLSLLGALVASYIFVFLILRNLNQWWFVTRHGRRYNLPPGDMGWPLIGTLLPFLQAFRS 63
Query: 63 GRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILT 122
GRPDSFIH FA KYGK+GMYKT+LFGSPSVIV VPEVCRHVLMNDEQF FGYSKATRILT
Sbjct: 64 GRPDSFIHLFAYKYGKIGMYKTYLFGSPSVIVTVPEVCRHVLMNDEQFGFGYSKATRILT 123
Query: 123 GSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLSE 182
GSKALNTVPK EHRRLRRLIASLISGNEALS+YIGHVEGIVVTCLEEW +MKKPVEFLSE
Sbjct: 124 GSKALNTVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWASMKKPVEFLSE 183
Query: 183 MKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELIR 242
MKTVAFKVLLHIFIGANTAAFID+MEKLY DFHLGFMS+ VDLPGTTFSRALKARNELI+
Sbjct: 184 MKTVAFKVLLHIFIGANTAAFIDRMEKLYNDFHLGFMSSPVDLPGTTFSRALKARNELIK 243
Query: 243 IFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETS 302
IFENVLKEKR NLK KV ENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETS
Sbjct: 244 IFENVLKEKRENLKKKVAENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETS 303
Query: 303 AHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDETL 362
AHSIMRAIMFLSENPETL KAKAEQEQIVKARPADAQHKGLTMKEIKQMEYL KVIDETL
Sbjct: 304 AHSIMRAIMFLSENPETLQKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLFKVIDETL 363
Query: 363 RKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPKR 422
RKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENP KFDPSRW+NPKR
Sbjct: 364 RKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWENPKR 423
Query: 423 RAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDNC 482
R GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGG+RLERVNPNCPEN+LPLPRP DNC
Sbjct: 424 RGGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDNC 483
Query: 483 LARVIRDS 491
LARV+RDS
Sbjct: 484 LARVVRDS 491
BLAST of Lag0033026 vs. TAIR 10
Match:
AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )
HSP 1 Score: 463.0 bits (1190), Expect = 3.0e-130
Identity = 228/470 (48.51%), Postives = 326/470 (69.36%), Query Frame = 0
Query: 21 ILRNLNQWRFVTRHG-GRYNLPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFASKYGKV 80
++R +N W + + G R+ LPPGD+GWP IG +L FL AF++ PDSF +YG
Sbjct: 25 LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84
Query: 81 GMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNTVPKAEHRRLR 140
G+YK H+FG+PS+IV + CR VL +D+ F G+ +T L G K+ + EH+RLR
Sbjct: 85 GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144
Query: 141 RLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLSEMKTVAFKVLLHIFIGAN 200
RL A+ ++G+EALS YI ++E V+T L++W M + EFL+ ++ + F+++++IF+ +
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204
Query: 201 TAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELIRIFENVLKEKRANLKNKV 260
+ +D +E+ YT + G + AV++PG + RALKAR L+ F++++ E+R K +
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264
Query: 261 TENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPET 320
N KKDM D LL V+DEDG+ LDD IID+L+ + AGHE+S H+IM A +FL E+PE
Sbjct: 265 LSN-KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324
Query: 321 LLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETKVDV 380
L +AKAEQE I+K+RP KGL++KE ++ME+LS+V+DETLR + + T RE K DV
Sbjct: 325 LQRAKAEQEMILKSRPEG--QKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDV 384
Query: 381 NLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDN---PKRRAGSFIPFGAGMRL 440
+NGY IPKGWK+L W R VH+DPE++ +P KFDP+RWDN PK AG+F+PFGAG L
Sbjct: 385 EMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHL 444
Query: 441 CPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDNCLARV 487
CPG DL+KLEI+IFLH+F+ Y+++R NP CP YLP RP DNCLAR+
Sbjct: 445 CPGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487
BLAST of Lag0033026 vs. TAIR 10
Match:
AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 457.6 bits (1176), Expect = 1.3e-128
Identity = 229/487 (47.02%), Postives = 332/487 (68.17%), Query Frame = 0
Query: 3 VWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHG-GRYNLPPGDMGWPIIGTLLPFLHAFR 62
+W LI G ++ + +L+ +N W +V++ G ++ LPPGD+GWP+IG + FL AF+
Sbjct: 9 MWFPLI--ILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 68
Query: 63 SGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRIL 122
+ P+SFI + ++YG+ G+YK H+FG P V+V PE CR VL +D+ F G+ K+T L
Sbjct: 69 TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 128
Query: 123 TGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLS 182
G K+ + EH+RLRRL ++ ++G EALS+YI +E V T LE+W M + +EFLS
Sbjct: 129 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 188
Query: 183 EMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELI 242
++ + FKV+++IF+ + + +D +E+ YT+ + G + ++LPG + RALKAR +L+
Sbjct: 189 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 248
Query: 243 RIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302
F++++ +R K ++ NR KDM D L++V+DE+G+ LDD IIDLL+ + AGHE+
Sbjct: 249 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 308
Query: 303 SAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDET 362
S H M A + + E+P L KAK EQE+IVK R A + LT+KE ++M YLS+VIDET
Sbjct: 309 SGHLTMWATILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDET 368
Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPK 422
LR + + T RE K DV ++GY IPKGWK+L W R VH+DPEIY +P KFDPSRW+
Sbjct: 369 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 428
Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDN 482
+AG+F+PFG G LCPG DL+KLEI+IFLH+F+ YR+ER NP CP +LP RP DN
Sbjct: 429 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486
Query: 483 CLARVIR 489
CLAR+ R
Sbjct: 489 CLARITR 486
BLAST of Lag0033026 vs. TAIR 10
Match:
AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 457.6 bits (1176), Expect = 1.3e-128
Identity = 229/487 (47.02%), Postives = 332/487 (68.17%), Query Frame = 0
Query: 3 VWLRLIGAFAGAYIFVFGILRNLNQWRFVTRHG-GRYNLPPGDMGWPIIGTLLPFLHAFR 62
+W LI G ++ + +L+ +N W +V++ G ++ LPPGD+GWP+IG + FL AF+
Sbjct: 9 MWFPLI--ILGLFVLKW-VLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFK 68
Query: 63 SGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEVCRHVLMNDEQFVFGYSKATRIL 122
+ P+SFI + ++YG+ G+YK H+FG P V+V PE CR VL +D+ F G+ K+T L
Sbjct: 69 TSDPESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKL 128
Query: 123 TGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGHVEGIVVTCLEEWRTMKKPVEFLS 182
G K+ + EH+RLRRL ++ ++G EALS+YI +E V T LE+W M + +EFLS
Sbjct: 129 IGRKSFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLS 188
Query: 183 EMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGFMSTAVDLPGTTFSRALKARNELI 242
++ + FKV+++IF+ + + +D +E+ YT+ + G + ++LPG + RALKAR +L+
Sbjct: 189 HLRKLTFKVIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLV 248
Query: 243 RIFENVLKEKRANLKNKVTENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHET 302
F++++ +R K ++ NR KDM D L++V+DE+G+ LDD IIDLL+ + AGHE+
Sbjct: 249 AAFQSIVTNRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHES 308
Query: 303 SAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLSKVIDET 362
S H M A + + E+P L KAK EQE+IVK R A + LT+KE ++M YLS+VIDET
Sbjct: 309 SGHLTMWATILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDET 368
Query: 363 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPHKFDPSRWDNPK 422
LR + + T RE K DV ++GY IPKGWK+L W R VH+DPEIY +P KFDPSRW+
Sbjct: 369 LRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYT 428
Query: 423 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGYRLERVNPNCPENYLPLPRPIDN 482
+AG+F+PFG G LCPG DL+KLEI+IFLH+F+ YR+ER NP CP +LP RP DN
Sbjct: 429 PKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDN 486
Query: 483 CLARVIR 489
CLAR+ R
Sbjct: 489 CLARITR 486
BLAST of Lag0033026 vs. TAIR 10
Match:
AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )
HSP 1 Score: 231.9 bits (590), Expect = 1.1e-60
Identity = 134/424 (31.60%), Postives = 225/424 (53.07%), Query Frame = 0
Query: 34 HGGRYNLPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVI 93
HG ++ P G +GWP+IG + F+ + S RP+SF+ YG+V +K+H+FG+ +++
Sbjct: 47 HGPKF--PHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRV--FKSHIFGTATIV 106
Query: 94 VCVPEVCRHVLMNDE-QFVFGYSKATRILTGSKALNTVPKAEHRRLRRLIASLISGNEAL 153
EV R VL +D FV Y K R L G ++ + + HRR L+ S +
Sbjct: 107 STDAEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLK 166
Query: 154 SIYIGHVEGIVVTCLEEWRTMKKPVEFLSEMKTVAFKVLLHIFIGANTAAFIDKMEKLYT 213
+ + + + ++ W + +PV KTVAFKVL I ++++++ +
Sbjct: 167 AQIVRDMHKFLSESMDLW-SEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFE 226
Query: 214 DFHLGFMSTAVDLPGTTFSRALKARNELIRIFENVLKEKRANLKNKVTEN-RKKDMTDLL 273
+F G MS ++ PGT R+L+A+ +++ E +++ K KNK ++ KD+ D+L
Sbjct: 227 NFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVL 286
Query: 274 LEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLLKAKAEQEQIV 333
L+ + + L I + +I GH++ I A+ FLS++P L E ++
Sbjct: 287 LK---DSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLK 346
Query: 334 KARPADAQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWK 393
+ + + L + + + KVI ETLR ++ + R+ DV + GY IPKGW
Sbjct: 347 SLK--ELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWC 406
Query: 394 ILVWTRAVHMDPEIYENPHKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFL 453
L + R+VH+D YE+P+KF+P RW SF PFG G RLCPG+DL++LE ++FL
Sbjct: 407 FLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFL 460
Query: 454 HYFI 456
H+ +
Sbjct: 467 HHLV 460
BLAST of Lag0033026 vs. TAIR 10
Match:
AT4G36380.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 219.5 bits (558), Expect = 5.8e-57
Identity = 134/421 (31.83%), Postives = 219/421 (52.02%), Query Frame = 0
Query: 40 LPPGDMGWPIIGTLLPFLHAFRSGRPDSFIHHFASKYGKVGMYKTHLFGSPSVIVCVPEV 99
+P G +GWP+IG L F+ S RP +F+ S YGKV +KT++ G+P +I EV
Sbjct: 69 IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKV--FKTNIIGTPIIISTDAEV 128
Query: 100 CRHVLMN-DEQFVFGYSKATRILTGSKALNTVPKAEHRRLRRLIASLISGNEALSIYIGH 159
+ VL N FV Y K+ L G ++ ++ +RL LI + +
Sbjct: 129 NKVVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRD 188
Query: 160 VEGIVVTCLEEWRTMKKPVEFLSEMKTVAFKVLLHIFIGANTAAFIDKMEKLYTDFHLGF 219
+E VV L W + V E+K + F++L+ + + + ++ ++ + +F G
Sbjct: 189 IEASVVLTLASWAQLPL-VHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGL 248
Query: 220 MSTAVDLPGTTFSRALKARNELIRIFENVLKEKRANLKNKVTENRKKDMTDLLL-EVRDE 279
+ + PGT ++LKA+ LI++ + V++E++ + T + D+ D+LL + D
Sbjct: 249 ICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAM---TTTSPANDVVDVLLRDGGDS 308
Query: 280 DGQGLDDGCIIDLLIGFFFAGHETSAHSIMRAIMFLSENPETLLKAKAEQEQIVKARPAD 339
+ Q + ++ G ET ++ A+ FLS+NP L K E+ +K R +
Sbjct: 309 EKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKL-VEENMEMKRRKLE 368
Query: 340 AQHKGLTMKEIKQMEYLSKVIDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTR 399
+ + + + VI+ETLR ++ + R+ DV + GY IPKGW +L
Sbjct: 369 LGEE-YKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFI 428
Query: 400 AVHMDPEIYENPHKFDPSRWDNPKRRAGS---FIPFGAGMRLCPGIDLSKLEIAIFLHYF 456
+VHMD +IY+NP++FDP RWD A S F PFG G RLCPG++LSKLEI+IFLH+
Sbjct: 429 SVHMDEDIYDNPYQFDPWRWDRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHL 481
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
B5BSX1 | 1.8e-140 | 51.67 | Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1 | [more] |
O23051 | 4.2e-129 | 48.51 | Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1 | [more] |
Q9C5Y2 | 1.8e-127 | 47.02 | Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2 | [more] |
Q9AXH9 | 7.4e-126 | 47.63 | Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1 | [more] |
Q5VRM7 | 9.1e-124 | 43.50 | Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1K3T5 | 4.8e-269 | 92.45 | beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111490917 PE=3 SV=... | [more] |
A0A6J1ET68 | 1.8e-268 | 92.24 | beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111437486 PE=3 S... | [more] |
A0A6J1GM12 | 2.0e-267 | 91.02 | beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455188 PE=3 S... | [more] |
A0A6J1I785 | 2.6e-267 | 90.61 | beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470633 PE=3 SV=... | [more] |
A0A5A7TAG0 | 1.0e-263 | 92.01 | Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... | [more] |