Lag0033015 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0033015 vs. NCBI nr
Match: XP_038877822.1 (tobamovirus multiplication protein 2A [Benincasa hispida])

HSP 1 Score: 538.1 bits (1385), Expect = 4.5e-149
Identity = 265/279 (94.98%), Postives = 275/279 (98.57%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRP 60
           MACRGCFECLLKLLNFFLS+LGLA+VGYGIYLLVEYLQSPSD+PGPSLSGDHD+VQLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSVLGLAVVGYGIYLLVEYLQSPSDVPGPSLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLL 120
           MLMAVSLSSN FDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYS+L+LLL
Sbjct: 61  MLMAVSLSSNFFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLLLLL 120

Query: 121 ILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFLL 180
           ILVQ GCAAFIFFDK+WRDEIP DKTGNFDKIYELLED+WEII+WVALGAVIFE LLFLL
Sbjct: 121 ILVQLGCAAFIFFDKNWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240
           ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR
Sbjct: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           MREKYGLDTSEFTYNPSESHR+QQVAPQPAEEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAPQPAEEKSRCTIM 279

BLAST of Lag0033015 vs. NCBI nr
Match: XP_022931040.1 (tobamovirus multiplication protein 2A-like [Cucurbita moschata] >KAG7036173.1 Tobamovirus multiplication protein 2A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 536.6 bits (1381), Expect = 1.3e-148
Identity = 265/279 (94.98%), Postives = 273/279 (97.85%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS SD+P P LSGDHDV+QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLL 120
           MLMAVSLSSNIFDNLP+AWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYS+LVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFLL 180
           ILVQ GCAAFIFFDKHWRDEIP DKTGNFDKIYELLED+WEII+WVALGAVIFE LLFLL
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240
           ALVVRAANRP+DYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR
Sbjct: 181 ALVVRAANRPLDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           MREKYGLDTSEFTYNPSESHR+QQVAPQPAEEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAPQPAEEKSRCTIM 279

BLAST of Lag0033015 vs. NCBI nr
Match: XP_023533384.1 (tobamovirus multiplication protein 2A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535.4 bits (1378), Expect = 2.9e-148
Identity = 264/279 (94.62%), Postives = 273/279 (97.85%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS SD+P P LSGDHDV+QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLL 120
           MLMAVSLSSNIFDNLP+AWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYS+LVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFLL 180
           ILVQ GCAAFIFFDKHWRDEIP D+TGNFDKIYELLED+WEII+WVALGAVIFE LLFLL
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDRTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240
           ALVVRAANRP+DYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR
Sbjct: 181 ALVVRAANRPLDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           MREKYGLDTSEFTYNPSESHR+QQVAPQPAEEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAPQPAEEKSRCTIM 279

BLAST of Lag0033015 vs. NCBI nr
Match: XP_022996390.1 (tobamovirus multiplication protein 2A-like [Cucurbita maxima])

HSP 1 Score: 534.3 bits (1375), Expect = 6.5e-148
Identity = 264/279 (94.62%), Postives = 271/279 (97.13%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS SD+P P LSGDHDV+QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLL 120
           MLMAVSLSSNIFDNLP+AWFIYLFIAVG IIFVVSCFGCIGAATRNGCCLSCYS+LVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGAIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFLL 180
           ILVQ GCAAFIFFDKHWRDEIP DKTGNFDKIYELLED+WEII+WVALGAVIFE LLFLL
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240
           ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWS R
Sbjct: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240

Query: 241 MREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           MREKYGLDTSEFTYNPSESHR+QQVAPQPAEEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAPQPAEEKSRCTIM 279

BLAST of Lag0033015 vs. NCBI nr
Match: XP_023553897.1 (tobamovirus multiplication protein 2A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 531.2 bits (1367), Expect = 5.5e-147
Identity = 264/279 (94.62%), Postives = 270/279 (96.77%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIP P LSGDHD+VQLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLL 120
           MLMAVSLSSNIFD+LPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYS+LVLLL
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFLL 180
           ILVQ GCAAFIFFDKHWRDEIP DKTGNFDKIYELLED+WEII+WVALGAVIFE LLFLL
Sbjct: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240
           ALVVRAAN P DYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAG LDQRPSRNDAWSTR
Sbjct: 181 ALVVRAANSPADYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGALDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           MREKYGLDTSEFTYNPSESHR+QQVA QPAEEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAQQPAEEKSRCTIM 279

BLAST of Lag0033015 vs. ExPASy Swiss-Prot
Match: Q9C5W7 (Tobamovirus multiplication protein 2A OS=Arabidopsis thaliana OX=3702 GN=TOM2A PE=1 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 2.8e-114
Identity = 199/280 (71.07%), Postives = 231/280 (82.50%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQ-SPSDIPGPSLSGDHDVVQLGR 60
           MACRGC ECLLKLLNF L++ GL M+GYGIYL VEY + + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLL 120
           P+LMAVSLSSNIFDNLPKAWFIYLFI +GV +FV+SC GC+G  +R+ CCLSCYS+L++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFL 180
           LILV+ G AAFIFFD  WRDE+P D+TGNFD IY  L + W+I++WVALGAV+FE LLFL
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

Query: 181 LALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWST 240
           LAL+VRAAN P +YDSDDEY+APRQQIRQP INR  AP TGVPVA TLDQRPSR+D WS 
Sbjct: 181 LALMVRAANTPAEYDSDDEYLAPRQQIRQPFINRQAAPVTGVPVAPTLDQRPSRSDPWSA 240

Query: 241 RMREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           RMREKYGLDTSEFTYNPSESHR+QQ+  QP EEK RCTIM
Sbjct: 241 RMREKYGLDTSEFTYNPSESHRFQQMPAQPNEEKGRCTIM 280

BLAST of Lag0033015 vs. ExPASy Swiss-Prot
Match: Q93XY5 (Tetraspanin-18 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH2 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 4.6e-32
Identity = 95/269 (35.32%), Postives = 142/269 (52.79%), Query Frame = 0

Query: 3   CRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS-PSDIP--GPSLSGDHDVVQLGR 62
           C   F   LK+LNF  + +G++++ Y I++L EY +  P D P    S SG      +  
Sbjct: 6   CHVSFASTLKILNFVQAFIGVSIIIYSIWMLHEYSRHLPVDPPPSASSSSGTEIATSVSE 65

Query: 63  PM------LMAVSLSSNIFD--------NLPKAWFIYLFIAVGVIIFVVSCFGCIGAATR 122
           P+      + ++ L SN  D        +LP  WFIY F+AVG+++ +V+  G I A   
Sbjct: 66  PLKNPIDFVASIILGSNGGDHGFNLRSLDLPAPWFIYSFMAVGILVCIVTFIGFIAAEAI 125

Query: 123 NGCCLSCYSILVLLLILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKW 182
           NGCCL  YSIL  LLIL++    A+I  D+HW  ++P D TG    +   +E+  +I KW
Sbjct: 126 NGCCLCFYSILKTLLILLEAALVAYIAIDRHWEKDLPYDPTGELSSLRAFIEENIDICKW 185

Query: 183 VALGAVIFEVLLFLLALVVRA----ANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGV 242
           V +  V  ++L  LLA+V+RA        +D + DDE   PR +   PL+     P    
Sbjct: 186 VGIAVVAVQLLSLLLAMVLRAMVSTPKPELDEEEDDE--NPRSRTWDPLL----GPQGNQ 245

Query: 243 PVAGTLDQRPSRNDAWSTRMREKYGLDTS 251
             AG+     S+ + WS+R+REKYGL+ S
Sbjct: 246 APAGS-----SKIENWSSRIREKYGLNQS 263

BLAST of Lag0033015 vs. ExPASy Swiss-Prot
Match: Q9SVU4 (Tetraspanin-20 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH1 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.1e-31
Identity = 89/285 (31.23%), Postives = 143/285 (50.18%), Query Frame = 0

Query: 3   CRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEY----------LQSP-SDIPGPSLSGD 62
           C   F  +LK+LNF  + +G++++ Y I++L +Y           Q P +  P  S S  
Sbjct: 6   CHVSFASILKILNFLQAFIGISIIIYSIWMLDQYNHHVPVDPPPSQPPAASSPDSSYSFS 65

Query: 63  HDVVQLGR---------PMLMAVSLSSNIFD--------NLPKAWFIYLFIAVGVIIFVV 122
           + ++++             +  + L S   D        +LP  WFIY F+A+G+++ +V
Sbjct: 66  NSIIEINSVSDSLKNPIDFVSGIVLGSGGGDSGFNLRSLDLPAPWFIYCFMAIGILVCIV 125

Query: 123 SCFGCIGAATRNGCCLSCYSILVLLLILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYE 182
           +  G I A   NGCCL  YSIL  LLI+++     FI  D+HW  ++P D TG  + +  
Sbjct: 126 TIIGFIAAEAINGCCLCFYSILKTLLIIIEAALVGFIVIDRHWEKDLPYDPTGELNSLRA 185

Query: 183 LLEDQWEIIKWVALGAVIFEVLLFLLALVVRA--ANRPVDYDSDDEYIAPRQQIRQPLIN 242
            +E+  +I KWV +  V  ++L  LLALV+RA  + R  + D +D++  P  + R  L+ 
Sbjct: 186 FIEENIDICKWVGIVVVAIQLLSLLLALVLRAMVSPRQSELDDEDDFENPMSRARDNLLG 245

Query: 243 RPVAPATGVPVAGTLDQRPSRNDAWSTRMREKYGLDTSEFTYNPS 258
                    P A       S  D W +R+REKYGL  S+ ++ PS
Sbjct: 246 ---------PQANQTSSGSSNIDNWRSRIREKYGLINSQ-SHTPS 280

BLAST of Lag0033015 vs. ExPASy Swiss-Prot
Match: Q940P5 (Tetraspanin-19 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH3 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 8.5e-26
Identity = 70/212 (33.02%), Postives = 114/212 (53.77%), Query Frame = 0

Query: 4   RGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRPMLM 63
           R C + +LKL+N  + ++G+AM+ Y ++L+ ++ +   ++  P    DH           
Sbjct: 6   RSCLQSMLKLVNSLIGMVGIAMILYAVWLIRQWQEQMGNL--PFADSDH----------- 65

Query: 64  AVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLLILV 123
                       P  WFIY F+ +G I+ VV+C G I A T NGCCL  Y   ++LL +V
Sbjct: 66  ------------PVPWFIYSFLGLGAILCVVTCAGHIAAETVNGCCLYLYMGFIVLLTMV 125

Query: 124 QGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFLLALV 183
           +GG  A IF ++ W+ + P+D +G F +  + +E  ++I KW+ L  V  + L  L+A++
Sbjct: 126 EGGVVADIFLNRDWKKDFPEDPSGAFHQFSKFIESNFKICKWIGLSIVCVQGLSVLIAML 185

Query: 184 VRA--ANRPVDYDSDDEY----IAPRQQIRQP 210
           ++A   +    YDSDDEY    +A  Q  RQP
Sbjct: 186 LKALGPHPHRHYDSDDEYNVSTVALLQDARQP 192

BLAST of Lag0033015 vs. ExPASy Swiss-Prot
Match: Q80WR1 (Tetraspanin-18 OS=Mus musculus OX=10090 GN=Tspan18 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.4e-04
Identity = 39/149 (26.17%), Postives = 71/149 (47.65%), Query Frame = 0

Query: 3   CRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRPML 62
           C  C + L+ + NFF+ L G  ++G GI++LV+             +G  ++V    P+L
Sbjct: 5   CLSCMKYLMFVFNFFVFLGGACLLGVGIWVLVD------------PTGFREIVAT-NPLL 64

Query: 63  MAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLLIL 122
              +               Y+ +A+G ++F++   GC GA   N C L  + + +L++ L
Sbjct: 65  TTGA---------------YIVLAMGGLLFLLGFLGCCGAVRENRCLLLFFFLFILIIFL 124

Query: 123 VQGGCA--AFIFFDKHWRDEIPKDKTGNF 150
           V+   A  AFIF +   R+   K+ T ++
Sbjct: 125 VELSAAILAFIFREHLTREFFTKELTKHY 125

BLAST of Lag0033015 vs. ExPASy TrEMBL
Match: A0A6J1EYE4 (tobamovirus multiplication protein 2A-like OS=Cucurbita moschata OX=3662 GN=LOC111437351 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 6.3e-149
Identity = 265/279 (94.98%), Postives = 273/279 (97.85%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS SD+P P LSGDHDV+QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLL 120
           MLMAVSLSSNIFDNLP+AWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYS+LVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFLL 180
           ILVQ GCAAFIFFDKHWRDEIP DKTGNFDKIYELLED+WEII+WVALGAVIFE LLFLL
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240
           ALVVRAANRP+DYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR
Sbjct: 181 ALVVRAANRPLDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           MREKYGLDTSEFTYNPSESHR+QQVAPQPAEEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAPQPAEEKSRCTIM 279

BLAST of Lag0033015 vs. ExPASy TrEMBL
Match: A0A6J1K6M6 (tobamovirus multiplication protein 2A-like OS=Cucurbita maxima OX=3661 GN=LOC111491627 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.1e-148
Identity = 264/279 (94.62%), Postives = 271/279 (97.13%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS SD+P P LSGDHDV+QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLL 120
           MLMAVSLSSNIFDNLP+AWFIYLFIAVG IIFVVSCFGCIGAATRNGCCLSCYS+LVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGAIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFLL 180
           ILVQ GCAAFIFFDKHWRDEIP DKTGNFDKIYELLED+WEII+WVALGAVIFE LLFLL
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240
           ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWS R
Sbjct: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240

Query: 241 MREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           MREKYGLDTSEFTYNPSESHR+QQVAPQPAEEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAPQPAEEKSRCTIM 279

BLAST of Lag0033015 vs. ExPASy TrEMBL
Match: A0A6J1GMT3 (tobamovirus multiplication protein 2A OS=Cucurbita moschata OX=3662 GN=LOC111455402 PE=4 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.3e-146
Identity = 263/279 (94.27%), Postives = 269/279 (96.42%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRP 60
           MACRGCFECLLKLLNF LSLLGLAMVGYGIYLLVEYLQSPSDIP P LSGDHD+VQLGRP
Sbjct: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLL 120
           MLMAVSLSSNIFD+LPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYS+LVLLL
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFLL 180
           ILVQ GCAAFIFFDKHWRDEIP DKTGNFDKIYELLED+WEII+WVALGAVIFE LLFLL
Sbjct: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240
           ALVVRAAN P DYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAG LDQRPSRNDAWSTR
Sbjct: 181 ALVVRAANSPADYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGALDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           MREKYGLDTSEFTYNPSESHR+QQVA QPAEEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAQQPAEEKSRCTIM 279

BLAST of Lag0033015 vs. ExPASy TrEMBL
Match: A0A6J1I3F8 (tobamovirus multiplication protein 2A-like OS=Cucurbita maxima OX=3661 GN=LOC111470643 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 1.7e-146
Identity = 263/279 (94.27%), Postives = 269/279 (96.42%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLV+YLQSPSDIP P LSGDHD+VQLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVDYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLL 120
           MLMAVSLSSNIFD+LPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYS+LVLLL
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFLL 180
           ILVQ GCAAFIFFDKHWRDEIP DKTGNFDKIYELLED+WEIIKWVALGAVIFE LLFLL
Sbjct: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIKWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240
           ALVVRAAN P DYDSDDEYIAPR QIRQPLINRPVAPATGVPVAG LDQRPSRNDAWSTR
Sbjct: 181 ALVVRAANSPADYDSDDEYIAPRLQIRQPLINRPVAPATGVPVAGALDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           MREKYGLDTSEFTYNPSESHR+QQVA QPAEEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAQQPAEEKSRCTIM 279

BLAST of Lag0033015 vs. ExPASy TrEMBL
Match: A0A5A7T8L3 (Tobamovirus multiplication protein 2A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00630 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.5e-145
Identity = 259/279 (92.83%), Postives = 271/279 (97.13%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSPSDIPGPSLSGDHDVVQLGRP 60
           MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS SD+PGPSLSGDHD+VQLGRP
Sbjct: 90  MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPGPSLSGDHDLVQLGRP 149

Query: 61  MLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLLL 120
           MLMAVSLSSNIFDNLPKAWFIYLFIA GVI+FVVSCFGCIGAATR+GCCLSCYSIL+LLL
Sbjct: 150 MLMAVSLSSNIFDNLPKAWFIYLFIATGVIVFVVSCFGCIGAATRSGCCLSCYSILLLLL 209

Query: 121 ILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFLL 180
           ILVQ GCAAFIFFDK+WRDEIP DKTGNFDKIYELLE++W+II+WVALG +IFE LLFLL
Sbjct: 210 ILVQLGCAAFIFFDKNWRDEIPGDKTGNFDKIYELLENKWKIIRWVALGTIIFEALLFLL 269

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240
           ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWS R
Sbjct: 270 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 329

Query: 241 MREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           MREKYGLDTSEFTYNPSESHR+QQVAPQ AEEKSRCTIM
Sbjct: 330 MREKYGLDTSEFTYNPSESHRFQQVAPQAAEEKSRCTIM 368

BLAST of Lag0033015 vs. TAIR 10
Match: AT1G32400.1 (tobamovirus multiplication 2A )

HSP 1 Score: 412.9 bits (1060), Expect = 2.0e-115
Identity = 199/280 (71.07%), Postives = 231/280 (82.50%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQ-SPSDIPGPSLSGDHDVVQLGR 60
           MACRGC ECLLKLLNF L++ GL M+GYGIYL VEY + + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLL 120
           P+LMAVSLSSNIFDNLPKAWFIYLFI +GV +FV+SC GC+G  +R+ CCLSCYS+L++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFL 180
           LILV+ G AAFIFFD  WRDE+P D+TGNFD IY  L + W+I++WVALGAV+FE LLFL
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

Query: 181 LALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWST 240
           LAL+VRAAN P +YDSDDEY+APRQQIRQP INR  AP TGVPVA TLDQRPSR+D WS 
Sbjct: 181 LALMVRAANTPAEYDSDDEYLAPRQQIRQPFINRQAAPVTGVPVAPTLDQRPSRSDPWSA 240

Query: 241 RMREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           RMREKYGLDTSEFTYNPSESHR+QQ+  QP EEK RCTIM
Sbjct: 241 RMREKYGLDTSEFTYNPSESHRFQQMPAQPNEEKGRCTIM 280

BLAST of Lag0033015 vs. TAIR 10
Match: AT1G32400.2 (tobamovirus multiplication 2A )

HSP 1 Score: 412.9 bits (1060), Expect = 2.0e-115
Identity = 199/280 (71.07%), Postives = 231/280 (82.50%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQ-SPSDIPGPSLSGDHDVVQLGR 60
           MACRGC ECLLKLLNF L++ GL M+GYGIYL VEY + + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLL 120
           P+LMAVSLSSNIFDNLPKAWFIYLFI +GV +FV+SC GC+G  +R+ CCLSCYS+L++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFL 180
           LILV+ G AAFIFFD  WRDE+P D+TGNFD IY  L + W+I++WVALGAV+FE LLFL
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

Query: 181 LALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWST 240
           LAL+VRAAN P +YDSDDEY+APRQQIRQP INR  AP TGVPVA TLDQRPSR+D WS 
Sbjct: 181 LALMVRAANTPAEYDSDDEYLAPRQQIRQPFINRQAAPVTGVPVAPTLDQRPSRSDPWSA 240

Query: 241 RMREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           RMREKYGLDTSEFTYNPSESHR+QQ+  QP EEK RCTIM
Sbjct: 241 RMREKYGLDTSEFTYNPSESHRFQQMPAQPNEEKGRCTIM 280

BLAST of Lag0033015 vs. TAIR 10
Match: AT1G32400.3 (tobamovirus multiplication 2A )

HSP 1 Score: 412.9 bits (1060), Expect = 2.0e-115
Identity = 199/280 (71.07%), Postives = 231/280 (82.50%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQ-SPSDIPGPSLSGDHDVVQLGR 60
           MACRGC ECLLKLLNF L++ GL M+GYGIYL VEY + + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNIFDNLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSILVLL 120
           P+LMAVSLSSNIFDNLPKAWFIYLFI +GV +FV+SC GC+G  +R+ CCLSCYS+L++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKWVALGAVIFEVLLFL 180
           LILV+ G AAFIFFD  WRDE+P D+TGNFD IY  L + W+I++WVALGAV+FE LLFL
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

Query: 181 LALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWST 240
           LAL+VRAAN P +YDSDDEY+APRQQIRQP INR  AP TGVPVA TLDQRPSR+D WS 
Sbjct: 181 LALMVRAANTPAEYDSDDEYLAPRQQIRQPFINRQAAPVTGVPVAPTLDQRPSRSDPWSA 240

Query: 241 RMREKYGLDTSEFTYNPSESHRYQQVAPQPAEEKSRCTIM 280
           RMREKYGLDTSEFTYNPSESHR+QQ+  QP EEK RCTIM
Sbjct: 241 RMREKYGLDTSEFTYNPSESHRFQQMPAQPNEEKGRCTIM 280

BLAST of Lag0033015 vs. TAIR 10
Match: AT2G20230.1 (Tetraspanin family protein )

HSP 1 Score: 139.8 bits (351), Expect = 3.3e-33
Identity = 95/269 (35.32%), Postives = 142/269 (52.79%), Query Frame = 0

Query: 3   CRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQS-PSDIP--GPSLSGDHDVVQLGR 62
           C   F   LK+LNF  + +G++++ Y I++L EY +  P D P    S SG      +  
Sbjct: 6   CHVSFASTLKILNFVQAFIGVSIIIYSIWMLHEYSRHLPVDPPPSASSSSGTEIATSVSE 65

Query: 63  PM------LMAVSLSSNIFD--------NLPKAWFIYLFIAVGVIIFVVSCFGCIGAATR 122
           P+      + ++ L SN  D        +LP  WFIY F+AVG+++ +V+  G I A   
Sbjct: 66  PLKNPIDFVASIILGSNGGDHGFNLRSLDLPAPWFIYSFMAVGILVCIVTFIGFIAAEAI 125

Query: 123 NGCCLSCYSILVLLLILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYELLEDQWEIIKW 182
           NGCCL  YSIL  LLIL++    A+I  D+HW  ++P D TG    +   +E+  +I KW
Sbjct: 126 NGCCLCFYSILKTLLILLEAALVAYIAIDRHWEKDLPYDPTGELSSLRAFIEENIDICKW 185

Query: 183 VALGAVIFEVLLFLLALVVRA----ANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGV 242
           V +  V  ++L  LLA+V+RA        +D + DDE   PR +   PL+     P    
Sbjct: 186 VGIAVVAVQLLSLLLAMVLRAMVSTPKPELDEEEDDE--NPRSRTWDPLL----GPQGNQ 245

Query: 243 PVAGTLDQRPSRNDAWSTRMREKYGLDTS 251
             AG+     S+ + WS+R+REKYGL+ S
Sbjct: 246 APAGS-----SKIENWSSRIREKYGLNQS 263

BLAST of Lag0033015 vs. TAIR 10
Match: AT4G28770.1 (Tetraspanin family protein )

HSP 1 Score: 136.3 bits (342), Expect = 3.6e-32
Identity = 89/285 (31.23%), Postives = 143/285 (50.18%), Query Frame = 0

Query: 3   CRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEY----------LQSP-SDIPGPSLSGD 62
           C   F  +LK+LNF  + +G++++ Y I++L +Y           Q P +  P  S S  
Sbjct: 6   CHVSFASILKILNFLQAFIGISIIIYSIWMLDQYNHHVPVDPPPSQPPAASSPDSSYSFS 65

Query: 63  HDVVQLGR---------PMLMAVSLSSNIFD--------NLPKAWFIYLFIAVGVIIFVV 122
           + ++++             +  + L S   D        +LP  WFIY F+A+G+++ +V
Sbjct: 66  NSIIEINSVSDSLKNPIDFVSGIVLGSGGGDSGFNLRSLDLPAPWFIYCFMAIGILVCIV 125

Query: 123 SCFGCIGAATRNGCCLSCYSILVLLLILVQGGCAAFIFFDKHWRDEIPKDKTGNFDKIYE 182
           +  G I A   NGCCL  YSIL  LLI+++     FI  D+HW  ++P D TG  + +  
Sbjct: 126 TIIGFIAAEAINGCCLCFYSILKTLLIIIEAALVGFIVIDRHWEKDLPYDPTGELNSLRA 185

Query: 183 LLEDQWEIIKWVALGAVIFEVLLFLLALVVRA--ANRPVDYDSDDEYIAPRQQIRQPLIN 242
            +E+  +I KWV +  V  ++L  LLALV+RA  + R  + D +D++  P  + R  L+ 
Sbjct: 186 FIEENIDICKWVGIVVVAIQLLSLLLALVLRAMVSPRQSELDDEDDFENPMSRARDNLLG 245

Query: 243 RPVAPATGVPVAGTLDQRPSRNDAWSTRMREKYGLDTSEFTYNPS 258
                    P A       S  D W +R+REKYGL  S+ ++ PS
Sbjct: 246 ---------PQANQTSSGSSNIDNWRSRIREKYGLINSQ-SHTPS 280