Lag0032908 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0032908 vs. NCBI nr
Match: XP_042974784.1 (uncharacterized protein LOC122306423 [Carya illinoinensis])

HSP 1 Score: 102.4 bits (254), Expect = 5.7e-18
Identity = 73/252 (28.97%), Postives = 122/252 (48.41%), Query Frame = 0

Query: 7    SSRDEITRFPNKKGTFTVKSAYHLVKDFNYGLEASQSDRMRSSLFWKSIWSLKALPRSKV 66
            +S D +     K G+F+VKSAY  ++  ++ L    S     ++FWKS+W LK   + K+
Sbjct: 843  NSEDSLYWSHEKNGSFSVKSAYRFIQQHHHLLYGQSSSGSSEAVFWKSLWHLKLPKKMKI 902

Query: 67   GVWKIVTDIIPTKANIQSKGIAIQASCAICGKGKETTSH-LINKIIHNNI-----IPAAK 126
              W+   + +PT  N++ + +   A+C +C +G E  +H L NK ++ NI     +    
Sbjct: 903  FAWRACQEKLPTYLNLKKRHVLDDATCVLCNQGMEDAAHALRNKQLYENISMPFNVTVNN 962

Query: 127  ALQITIEVNIKERIFTFDKQQNSRTRSQVSQAT-WERPKSNQWKLNSDAGWFEKDGRGGI 186
            AL +  E    E++  FD      +  ++S+   W  P S+  KLN D   F +    G+
Sbjct: 963  ALALEKEY---EQVHLFD-----ASNPKISKVVRWHPPPSDFLKLNIDGATFPELSVAGV 1022

Query: 187  GWIVRDSTGSTMCFGMKAITNPWPIKMLEAQAILEGLNCIKDTCKQRQIP-LVIESDTLE 246
            G ++R+  G  +    K        + +EA A+L GL      C Q  IP L++E+D L 
Sbjct: 1023 GVVLRNHKGEVIVACSKVEKEVSSAEFIEAVALLRGL----QLCVQWGIPKLMLETDCLI 1082

Query: 247  VIKVLNKEEEDL 251
            ++  LN   E L
Sbjct: 1083 LVNALNGNSECL 1082

BLAST of Lag0032908 vs. NCBI nr
Match: KAF7837133.1 (uncharacterized protein G2W53_005615 [Senna tora])

HSP 1 Score: 97.1 bits (240), Expect = 2.4e-16
Identity = 71/239 (29.71%), Postives = 119/239 (49.79%), Query Frame = 0

Query: 17  NKKGTFTVKSAYHLVKDFNYGLEASQSDRMRSSLFWKSIWSLKALPRSKVGVWKIVTDII 76
           N  G F+VKSAY +V    +   +  +   R S  WK IW  K  P+ +   W+    I+
Sbjct: 26  NANGLFSVKSAYKVV----HNSYSRTNTWTRYSSVWKKIWKAKLPPKIRSFCWRAYRGIL 85

Query: 77  PTKANIQSKGIAIQASCAICGKGKETTSHLI-----NKIIHNNIIPAAKALQITIEVNIK 136
           PT  N++++G+ +   C +CG   E TSH +      K + +N +  A     T EV + 
Sbjct: 86  PTCVNLKNRGMEVDTRCCVCGLEDENTSHALVGCPELKSLWDNHMSDADGQFNTNEVFV- 145

Query: 137 ERIFTFDKQQNSRTRSQVSQATWERPKSNQWKLNSDAGWFEKDGRGGIGWIVRDSTGSTM 196
           E       +++ R R+ + +  WE P  ++ K+N DA  F   G+GGIG +VR+  GS +
Sbjct: 146 EWFSNKGTRRDDRNRA-IMEGQWEAPMWHEMKVNVDAS-FLGQGKGGIGCVVRNFQGSCL 205

Query: 197 CFGMKAITNPWPIKMLEAQAILEGLNCIKDTCKQRQIPLVIESDTLEVIKVLNKEEEDL 251
               K I N     +LEA A L+GL   K+    R   +++E +  +V+ +++   ++L
Sbjct: 206 AAFAKRIQNIESPDLLEAYAFLKGLELAKNL---RCDNIILEGEAKKVVDLIHGSSQNL 254

BLAST of Lag0032908 vs. NCBI nr
Match: XP_011470502.1 (PREDICTED: uncharacterized protein LOC105353223 [Fragaria vesca subsp. vesca])

HSP 1 Score: 94.7 bits (234), Expect = 1.2e-15
Identity = 61/242 (25.21%), Postives = 113/242 (46.69%), Query Frame = 0

Query: 17  NKKGTFTVKSAYHLVKDFNY--GLEASQSDRMRSSLFWKSIWSLKALPRSKVGVWKIVTD 76
           ++KG ++V+S YH+ +   +   + +S S        WK +W +   P+ ++  W+I+ +
Sbjct: 56  DQKGLYSVRSGYHVARKVEHLTTIASSSSSHGGWRSIWKKVWKVNVPPKVRLHAWRILRE 115

Query: 77  IIPTKANIQSKGIAIQASCAICGKGKETTSHLINKIIHNNIIPAAKALQITIE----VNI 136
           ++PTK  ++ KG+ +   C  CG+ KE   H+       N +    +L +T      + I
Sbjct: 116 VLPTKWALKKKGVDLSVECPFCGRDKEDGLHIFKSCRVVNQLWECSSLTLTPSTHPAMEI 175

Query: 137 KERIFTFDKQQ-NSRTRSQVSQATWERPKSNQWKLNSDAGWFEKDGRGGIGWIVRDSTGS 196
           K  +  + K    +  +S+ +   W  P S + K+N D  + E   RGG+G +VRD+ G 
Sbjct: 176 KAWVDEYIKYHCRTGKQSRRTHTKWRCPPSGRLKINIDGSFIELQERGGMGVVVRDNNGD 235

Query: 197 TMCFGMKAITNPWPIKMLEAQAILEGLNC-IKDTCKQRQIPLVIESDTLEVIKVLNKEEE 251
                  A   P P+ +      +E L   +    +Q    +  ESD   ++KV   ++E
Sbjct: 236 CRA----AFARPIPVALSALHTEVEALRAGLLIAVQQGWDEVEFESDCAALVKVFQGDDE 293

BLAST of Lag0032908 vs. NCBI nr
Match: XP_030930729.1 (uncharacterized protein LOC115956517 [Quercus lobata])

HSP 1 Score: 91.7 bits (226), Expect = 1.0e-14
Identity = 67/253 (26.48%), Postives = 123/253 (48.62%), Query Frame = 0

Query: 10  DEITRFPNKKGTFTVKSAYHLVKDF----NYGLEASQSDRMRSSLFWKSIWSLKALPRSK 69
           D I    NKKG+F VKSAY++ K      ++G E+S  D   S   WK +W L    + +
Sbjct: 113 DSIIWLGNKKGSFLVKSAYYVAKRILEKEDHG-ESSLGDVCAS--LWKRMWHLNIPGKIR 172

Query: 70  VGVWKIVTDIIPTKANIQSKGIAIQASCAICGKGKETTSHLI--------NKIIHNN--- 129
           +  W++  + IPT  N+  KGI +  +C IC K  ET  H I        N++++ N   
Sbjct: 173 IFAWRLCMNAIPTLTNLFKKGIQMDVTCPICKKDPETVEHAIISVSAKAVNQVVNENGGV 232

Query: 130 IIPAAKALQITIEVNIKERIFTFDKQQNSRTRSQVSQATWERPKSNQWKLNSDAGWFEKD 189
           +      L I +  + KE      +++ ++ R+      W+ P  + + +N D     K+
Sbjct: 233 VAELVWGLAIRLIEDFKEANIQIIEKKGTKDRA------WKPPPKDVYLINVDGAIPAKE 292

Query: 190 GRGGIGWIVRDSTGSTMCFGMKAITNPWPIKMLEAQAILEGLNCIKDTCKQRQIPLVIES 247
           G  GIG I+RD     +    K ++  + ++  EA A+ +G+   K+    +   +++E 
Sbjct: 293 GNSGIGVIIRDWNNQVVAALSKPLSGRFAVEETEAIAMEQGIVLAKELGLNQ---IILEG 352

BLAST of Lag0032908 vs. NCBI nr
Match: OMO52016.1 (reverse transcriptase [Corchorus capsularis])

HSP 1 Score: 89.7 bits (221), Expect = 3.8e-14
Identity = 64/263 (24.33%), Postives = 119/263 (45.25%), Query Frame = 0

Query: 9    RDEITRFPNKKGTFTVKSAYHLVKDFNYGLEASQSDRMRSSLFWKSIWSLKALPRSKVGV 68
            RD +     + G F+VKSAY++ +D     ++ Q  R ++   WK +WS + +P+     
Sbjct: 1115 RDCLIWNATRMGEFSVKSAYYVARDVLRRADSLQGPRQQT---WKLVWSSQIMPKIHFFT 1174

Query: 69   WKIVTDIIPTKANIQSKGIAIQASCAICGKGKETTSHL-----INKIIHNNIIPA----- 128
            W+++ +I+PTK N+  +G+ +   C +CGK  E+  H+      ++++ + + P      
Sbjct: 1175 WRLIWNILPTKGNLVFRGLDVPQLCEVCGKQPESVFHVFFRCKFSELVWDRVGPCVNLTL 1234

Query: 129  --------------AKALQITIEVNIKERIFTFDKQQNSRTRSQVSQATWERPKSNQWKL 188
                          AKA  I     + + + T     N+R ++   Q TW  P     K+
Sbjct: 1235 DQWNSDGDWWDFFIAKATSIG---QLDKVLITLWLIWNNRNKALYEQLTWIPPPMGIVKI 1294

Query: 189  NSDAGWFEKDGRGGIGWIVRDSTGSTMCFGMKAITNPWPIKMLEAQAILEGLNCIKDTCK 248
            N+D  +    G  G+G ++RDS G  +    + +         E  A+L G     +   
Sbjct: 1295 NTDVAFCSSSGEAGLGVVIRDSAGKIIICASRCLNFIADSLYAEVHALLFGFELAIEHGI 1354

BLAST of Lag0032908 vs. ExPASy TrEMBL
Match: A0A1R3G1P9 (Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_29422 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.8e-14
Identity = 64/263 (24.33%), Postives = 119/263 (45.25%), Query Frame = 0

Query: 9    RDEITRFPNKKGTFTVKSAYHLVKDFNYGLEASQSDRMRSSLFWKSIWSLKALPRSKVGV 68
            RD +     + G F+VKSAY++ +D     ++ Q  R ++   WK +WS + +P+     
Sbjct: 1115 RDCLIWNATRMGEFSVKSAYYVARDVLRRADSLQGPRQQT---WKLVWSSQIMPKIHFFT 1174

Query: 69   WKIVTDIIPTKANIQSKGIAIQASCAICGKGKETTSHL-----INKIIHNNIIPA----- 128
            W+++ +I+PTK N+  +G+ +   C +CGK  E+  H+      ++++ + + P      
Sbjct: 1175 WRLIWNILPTKGNLVFRGLDVPQLCEVCGKQPESVFHVFFRCKFSELVWDRVGPCVNLTL 1234

Query: 129  --------------AKALQITIEVNIKERIFTFDKQQNSRTRSQVSQATWERPKSNQWKL 188
                          AKA  I     + + + T     N+R ++   Q TW  P     K+
Sbjct: 1235 DQWNSDGDWWDFFIAKATSIG---QLDKVLITLWLIWNNRNKALYEQLTWIPPPMGIVKI 1294

Query: 189  NSDAGWFEKDGRGGIGWIVRDSTGSTMCFGMKAITNPWPIKMLEAQAILEGLNCIKDTCK 248
            N+D  +    G  G+G ++RDS G  +    + +         E  A+L G     +   
Sbjct: 1295 NTDVAFCSSSGEAGLGVVIRDSAGKIIICASRCLNFIADSLYAEVHALLFGFELAIEHGI 1354

BLAST of Lag0032908 vs. ExPASy TrEMBL
Match: A0A6P9E8H5 (uncharacterized protein LOC118347698 OS=Juglans regia OX=51240 GN=LOC118347698 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.8e-14
Identity = 75/256 (29.30%), Postives = 113/256 (44.14%), Query Frame = 0

Query: 9   RDEITRFPNKKGTFTVKSAYHLVKDFNYGLEASQSDRMRSSLFWKSIWSLKALPRSKVGV 68
           +D++    +K G F+V SAY L  D    L+   S        W  IW+L      K+ +
Sbjct: 570 QDKLFWGSSKIGEFSVGSAYFLQLDRLKSLQGESSREGSKDEKWSIIWNLTVPNSVKIFL 629

Query: 69  WKIVTDIIPTKANIQSKGIAIQASCAICGKGKETTSHLINKIIHNNIIPA---------- 128
           WK+  +++PTK N+  + I  + SC IC    ET  H I +    N I A          
Sbjct: 630 WKVGNNLLPTKENLFKRKIVTEKSCTICFAESETIMHAIWECPAANDIWADKDRFPKTVL 689

Query: 129 AKALQITIEVNIKERIFTFDKQQNSRTRSQVSQATWERPKSNQWKLNSDAGWFEKDGRGG 188
            KA +  +E    + +    KQ   R R  +    WE+P +   K+N DA    KD R G
Sbjct: 690 QKASEPLVEYKAAQALLQQTKQSQVRGRGDLK---WEKPAAGLVKINWDASLDVKDRRMG 749

Query: 189 IGWIVRDSTGSTM---CFGMKAITNPWPIKMLEAQAILEGLNCIKDTCKQRQIP-LVIES 248
           +G I+RD  G  +   C   + + NP    + E+ A+   L    D C +  I   + E 
Sbjct: 750 VGIIIRDEEGEALVAVCDQKEHVDNP---MVAESYALRVAL----DVCGELNIQRAIFEG 809

Query: 249 DTLEVIKVLNKEEEDL 251
           D   VI  +++E EDL
Sbjct: 810 DAKSVITAVHREAEDL 815

BLAST of Lag0032908 vs. ExPASy TrEMBL
Match: A0A6J5UV01 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS32434 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.1e-14
Identity = 72/257 (28.02%), Postives = 119/257 (46.30%), Query Frame = 0

Query: 20   GTFTVKSAY----HLVKDFNYGL----EASQSDRMRSSLFWKSIWSLKALPRSKVGVWKI 79
            G +TV+S Y     L ++   G     E+SQ D+ R    WKSIW L+  P+ +  +WK 
Sbjct: 898  GGYTVRSGYAVALTLRRNGELGRKAEGESSQGDKQRR--IWKSIWGLQVPPKIRTFLWKC 957

Query: 80   VTDIIPTKANIQSKGIAIQASCAICGKGKETTSHLI---------NKIIHNNIIPAAKAL 139
              +++  K N+   GI ++ +CA+CG+  E+   ++          ++ H N I     +
Sbjct: 958  CRNVLAVKENLLHWGIDVETTCALCGQEGESQDFIVCWENLLKKYEQVEHCNDILQGTVI 1017

Query: 140  QITIEVN-IKERIFTFDKQQ-------NSRTRSQVSQA----TWERPKSNQWKLNSDAGW 199
            +  + V     ++  F   Q       N   R    QA    +W +      K+N DA W
Sbjct: 1018 EPHVAVECFLNQVHEFRTAQMHGKPLINELPRPPEIQAETVTSWRKLPYGVLKVNCDAAW 1077

Query: 200  FEKDGRGGIGWIVRDSTGSTMCFGMKAITNPWPIKMLEAQAILEG-LNCIKDTCKQRQIP 247
              +   GG+GW+VRDS G  +  G K          +EA+AI E  L C++    +    
Sbjct: 1078 VSQTQMGGVGWVVRDSYGWMVQAGCKGDMRGGSALAMEAKAIREALLACVQSGLSK---- 1137

BLAST of Lag0032908 vs. ExPASy TrEMBL
Match: Q75M12 (Reverse transcriptase domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=P0431G05.11 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 5.3e-14
Identity = 60/240 (25.00%), Postives = 106/240 (44.17%), Query Frame = 0

Query: 10   DEITRFPNKKGTFTVKSAYHLVKDFNYGLEASQSDRMRSSLFWKSIWSLKALPRSKVGVW 69
            D I   P+K G F+V+SAY L          S S   R +  W+ IW      + ++  W
Sbjct: 1639 DFIAWHPDKTGRFSVRSAYKLALQLADMNNCSSSSSSRLNKSWELIWKCNVPQKVRIFAW 1698

Query: 70   KIVTDIIPTKANIQSKGIAIQASCAICGKGKETTSHLINKIIHNNIIPAAKALQITIEVN 129
            ++ ++ + T  N + + +     C IC + KE   H + + +H      A +L + +E  
Sbjct: 1699 RVASNSLATMENKKKRNLERFDVCGICDREKEDAGHALCRCVH------ANSLWVNLEKG 1758

Query: 130  IKERIFTFDKQQNSRTRSQVSQATWERPKSNQWKLNSDAGWFEKDGRGGIGWIVRDSTGS 189
                   F K    +         WERP++   KLN D  +     +GGIG I+R+  G+
Sbjct: 1759 KYVVSVNFPKTTAIKHTPGAENRRWERPRNGWMKLNVDGSFDINSEKGGIGMILRNCLGN 1818

Query: 190  TMCFGMKAITNPWPIKMLEAQAILEGLNCIKDTCKQRQIPLVIESDTLEVIKVLNKEEED 249
             +    +++ +       E  A +EGL+          +P+ +E+D   VI++LN  ++D
Sbjct: 1819 VIFSSCRSLDSCSGPLEAELHACVEGLHL---ALHWTLLPIQVETDCSSVIQLLNHPDKD 1869

BLAST of Lag0032908 vs. ExPASy TrEMBL
Match: A0A1R3IUT4 (Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_09629 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 4.5e-13
Identity = 57/231 (24.68%), Postives = 105/231 (45.45%), Query Frame = 0

Query: 20  GTFTVKSAYHLVKDFNYGLEASQSDRMRSSLFWKSIWSLKALPRSKVGVWKIVTDIIPTK 79
           G++TV+S Y++ +      +    DR   S  W+ IWS   LP+ K   W+++ DI+PTK
Sbjct: 626 GSYTVRSGYYVARSLLGHDDIHVDDR---SPVWRLIWSAHVLPKVKYFFWRVIQDILPTK 685

Query: 80  ANIQSKGIAIQASCAICGKGKETTSHLINKIIHNNIIPAAKALQITIEVNIKERIFTFDK 139
             +Q +G+ I  +C +CG  + +  H+  +   +  +       + + +   E   +F +
Sbjct: 686 PQLQRRGVPIDLNCVVCGGNESSLYHVFFECTFSRQVWVIACPWLPVYLESYEYGASFRR 745

Query: 140 QQNSRTRSQVSQATWERPKSNQWKLNSDAGWFEKDGRGGIGWIVRDSTGSTMCFGMKAIT 199
            + S+T   + +  W  P   + K+N+DA + +      +G + RD+ G  +  G+  IT
Sbjct: 746 DRQSQT---IVRQQWTPPTQGRVKINTDAAFSQHRQEAALGVVFRDTDGHVLLSGITRIT 805

Query: 200 NPWPIKMLEAQAILEGLNCIKDTCKQRQIPLVIESDTLEVIKVLNKEEEDL 251
           N       E   I  GL           +   +ESD+L     LN++   L
Sbjct: 806 NVPNSLFAEMYTIRFGLLL---ALSYSFVTCEVESDSLLANIELNRQTPSL 847

BLAST of Lag0032908 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 4.1e-06
Identity = 38/108 (35.19%), Postives = 61/108 (56.48%), Query Frame = 0

Query: 145 TRSQVSQAT---WERPKSNQW-KLNSDAGWFEKDGRGGIGWIVRDSTGSTMCFGMKAITN 204
           T+ QV++++   W RP  +QW K N+DA W   + R GIGW++R+  G     G +A+  
Sbjct: 408 TKPQVNRSSCGRW-RPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARAL-- 467

Query: 205 PWPIKMLEAQAILEGLNCIKDTCKQRQIPLVI-ESDTLEVIKVLNKEE 248
           P    +LEA+  LE +     +  + Q   VI ESD+  +I++LN +E
Sbjct: 468 PKLKSVLEAE--LEAMRWAVLSLSRFQYNYVIFESDSQVLIEILNNDE 510

BLAST of Lag0032908 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 4.5e-05
Identity = 29/95 (30.53%), Postives = 50/95 (52.63%), Query Frame = 0

Query: 154 WERPKSNQWKLNSDAGWFEKDGRGGIGWIVRDSTGSTMCFGMKAITNPWPIKMLEAQAIL 213
           W+ P     K N+DA W  ++ R GIGWI+R+ +G  +  G +A+  P    +LEA+  L
Sbjct: 137 WKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARAL--PRTKNVLEAE--L 196

Query: 214 EGLN-CIKDTCKQRQIPLVIESDTLEVIKVLNKEE 248
           E L   +    +     ++ ESD   ++ +LN ++
Sbjct: 197 EALRWAVLTMSRFNYKRIIFESDAQALVNLLNSDD 227

BLAST of Lag0032908 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 43.1 bits (100), Expect = 3.8e-04
Identity = 19/53 (35.85%), Postives = 33/53 (62.26%), Query Frame = 0

Query: 55  IWSLKALPRSKVGVWKIVTDIIPTKANIQSKGIAIQASCAICGKGKETTSHLI 108
           IWSLK  P+ K+ +WK + + +P  A + S+ I+I+  C  C +  ET +H++
Sbjct: 9   IWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRC-RDFETITHIL 60