Lag0032904 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032904
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description14 kDa proline-rich protein DC2.15-like
Locationchr11: 38777009 .. 38777422 (+)
RNA-Seq ExpressionLag0032904
SyntenyLag0032904
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTAAAGCTACAGCCTCTGTTGCCTTCCTCCTCTCTCTCAATCTCCTCTTCTTCTCCCTTGTCTCAGCCTGCAACAACTGCTATGTCCCTGCCCCACCCAAGCCCAAGCCATGCCCTCCAGTGCCAAAGCCCACCCCTTCATCTGGCTATGGAAAGTGCCCTAGGGACACCCTTAAACTCGGCGTCTGCGCCAAGCTCCTCGGTGGCCTCGTCGACGTTACCATCGGCAAGCCCCCGGTGGCGCCGTGCTGCAGCCTGATCGAAGGCCTTGCTGATCTTGAAGCTGCTGTCTGCCTTTGCACTGCCATTAAGGCCAATGTTTTGGGCAACAAACTCAACATCCCTGTCTCACTCAGCTTGCTTCTCAATGTTTGCAAGAAGAATGTTCCCAAGGGATTCAAGTGCTGA

mRNA sequence

ATGGCTTCTAAAGCTACAGCCTCTGTTGCCTTCCTCCTCTCTCTCAATCTCCTCTTCTTCTCCCTTGTCTCAGCCTGCAACAACTGCTATGTCCCTGCCCCACCCAAGCCCAAGCCATGCCCTCCAGTGCCAAAGCCCACCCCTTCATCTGGCTATGGAAAGTGCCCTAGGGACACCCTTAAACTCGGCGTCTGCGCCAAGCTCCTCGGTGGCCTCGTCGACGTTACCATCGGCAAGCCCCCGGTGGCGCCGTGCTGCAGCCTGATCGAAGGCCTTGCTGATCTTGAAGCTGCTGTCTGCCTTTGCACTGCCATTAAGGCCAATGTTTTGGGCAACAAACTCAACATCCCTGTCTCACTCAGCTTGCTTCTCAATGTTTGCAAGAAGAATGTTCCCAAGGGATTCAAGTGCTGA

Coding sequence (CDS)

ATGGCTTCTAAAGCTACAGCCTCTGTTGCCTTCCTCCTCTCTCTCAATCTCCTCTTCTTCTCCCTTGTCTCAGCCTGCAACAACTGCTATGTCCCTGCCCCACCCAAGCCCAAGCCATGCCCTCCAGTGCCAAAGCCCACCCCTTCATCTGGCTATGGAAAGTGCCCTAGGGACACCCTTAAACTCGGCGTCTGCGCCAAGCTCCTCGGTGGCCTCGTCGACGTTACCATCGGCAAGCCCCCGGTGGCGCCGTGCTGCAGCCTGATCGAAGGCCTTGCTGATCTTGAAGCTGCTGTCTGCCTTTGCACTGCCATTAAGGCCAATGTTTTGGGCAACAAACTCAACATCCCTGTCTCACTCAGCTTGCTTCTCAATGTTTGCAAGAAGAATGTTCCCAAGGGATTCAAGTGCTGA

Protein sequence

MASKATASVAFLLSLNLLFFSLVSACNNCYVPAPPKPKPCPPVPKPTPSSGYGKCPRDTLKLGVCAKLLGGLVDVTIGKPPVAPCCSLIEGLADLEAAVCLCTAIKANVLGNKLNIPVSLSLLLNVCKKNVPKGFKC
Homology
BLAST of Lag0032904 vs. NCBI nr
Match: XP_022922126.1 (14 kDa proline-rich protein DC2.15-like [Cucurbita moschata] >XP_023550963.1 14 kDa proline-rich protein DC2.15-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 259.6 bits (662), Expect = 1.5e-65
Identity = 131/139 (94.24%), Postives = 134/139 (96.40%), Query Frame = 0

Query: 1   MASKATASVAFLLSLNLLFFSLVSACNNCYVPAPPKPKPC--PPVPKPTPSSGYGKCPRD 60
           MASKATAS+AFLLSLNLLFFSLVSACNNCYVPAPPKPKPC  PP PKPTPSSGYGKCPRD
Sbjct: 1   MASKATASLAFLLSLNLLFFSLVSACNNCYVPAPPKPKPCPPPPYPKPTPSSGYGKCPRD 60

Query: 61  TLKLGVCAKLLGGLVDVTIGKPPVAPCCSLIEGLADLEAAVCLCTAIKANVLGNKLNIPV 120
           TLKLGVCAKLLGGLVDVTIGKPPV PCCSLIEGLADLEAAVCLCTAIKA+VLGN LNIPV
Sbjct: 61  TLKLGVCAKLLGGLVDVTIGKPPVTPCCSLIEGLADLEAAVCLCTAIKASVLGNNLNIPV 120

Query: 121 SLSLLLNVCKKNVPKGFKC 138
           SLSLLLNVCKKNVPKGF+C
Sbjct: 121 SLSLLLNVCKKNVPKGFQC 139

BLAST of Lag0032904 vs. NCBI nr
Match: XP_022970161.1 (14 kDa proline-rich protein DC2.15-like [Cucurbita maxima])

HSP 1 Score: 259.6 bits (662), Expect = 1.5e-65
Identity = 130/138 (94.20%), Postives = 134/138 (97.10%), Query Frame = 0

Query: 1   MASKATASVAFLLSLNLLFFSLVSACNNCYVPAPPKPKPC-PPVPKPTPSSGYGKCPRDT 60
           MASKATAS+AFLLSLNLLFFSLVSACNNCYVPAPPKPKPC PP PKPTPSSGYGKCPRDT
Sbjct: 1   MASKATASLAFLLSLNLLFFSLVSACNNCYVPAPPKPKPCPPPYPKPTPSSGYGKCPRDT 60

Query: 61  LKLGVCAKLLGGLVDVTIGKPPVAPCCSLIEGLADLEAAVCLCTAIKANVLGNKLNIPVS 120
           LKLGVCAKLLGGL+DVTIGKPPV PCCSLIEGLADLEAAVCLCTAIKA+VLGN LNIPVS
Sbjct: 61  LKLGVCAKLLGGLIDVTIGKPPVTPCCSLIEGLADLEAAVCLCTAIKASVLGNNLNIPVS 120

Query: 121 LSLLLNVCKKNVPKGFKC 138
           LSLLLNVCKKNVPKGF+C
Sbjct: 121 LSLLLNVCKKNVPKGFQC 138

BLAST of Lag0032904 vs. NCBI nr
Match: XP_038906980.1 (14 kDa proline-rich protein DC2.15-like [Benincasa hispida])

HSP 1 Score: 256.1 bits (653), Expect = 1.7e-64
Identity = 126/137 (91.97%), Postives = 133/137 (97.08%), Query Frame = 0

Query: 1   MASKATASVAFLLSLNLLFFSLVSACNNCYVPAPPKPKPCPPVPKPTPSSGYGKCPRDTL 60
           MASKATAS+AFLLSLNLLFFSLVSACNNCYVPAPPKPKPCPPV KP+PSSGYGKCPRDTL
Sbjct: 1   MASKATASLAFLLSLNLLFFSLVSACNNCYVPAPPKPKPCPPVVKPSPSSGYGKCPRDTL 60

Query: 61  KLGVCAKLLGGLVDVTIGKPPVAPCCSLIEGLADLEAAVCLCTAIKANVLGNKLNIPVSL 120
           KLGVCAKLLGGLVD+TIGKPPV PCCSLIEGLADLEAAVCLCTAIKAN+LG  LN+PVSL
Sbjct: 61  KLGVCAKLLGGLVDITIGKPPVTPCCSLIEGLADLEAAVCLCTAIKANLLGLNLNLPVSL 120

Query: 121 SLLLNVCKKNVPKGFKC 138
           SLLLNVC+KNVPKGF+C
Sbjct: 121 SLLLNVCQKNVPKGFQC 137

BLAST of Lag0032904 vs. NCBI nr
Match: XP_008437101.1 (PREDICTED: 14 kDa proline-rich protein DC2.15-like [Cucumis melo] >KAA0039707.1 14 kDa proline-rich protein DC2.15-like [Cucumis melo var. makuwa])

HSP 1 Score: 249.2 bits (635), Expect = 2.0e-62
Identity = 125/140 (89.29%), Postives = 131/140 (93.57%), Query Frame = 0

Query: 1   MASKATASVAFLLSLNLLFFSLVSACNNCYVPAPPKPKPCP---PVPKPTPSSGYGKCPR 60
           MASKAT S+AFLLSLNLLFF+LVSACNNCYVP PPKPKPCP   P PKP+PSSGYGKCPR
Sbjct: 1   MASKATTSLAFLLSLNLLFFTLVSACNNCYVPEPPKPKPCPPTKPTPKPSPSSGYGKCPR 60

Query: 61  DTLKLGVCAKLLGGLVDVTIGKPPVAPCCSLIEGLADLEAAVCLCTAIKANVLGNKLNIP 120
           DTLKLGVCAKLLGGLVDVTIGKPPV PCCSLIEGLADLEAAVCLCTAIKAN+LG  LNIP
Sbjct: 61  DTLKLGVCAKLLGGLVDVTIGKPPVTPCCSLIEGLADLEAAVCLCTAIKANLLGLNLNIP 120

Query: 121 VSLSLLLNVCKKNVPKGFKC 138
           VSLSLLLNVC+KNVPKGF+C
Sbjct: 121 VSLSLLLNVCQKNVPKGFQC 140

BLAST of Lag0032904 vs. NCBI nr
Match: XP_004152410.2 (14 kDa proline-rich protein DC2.15 [Cucumis sativus])

HSP 1 Score: 244.2 bits (622), Expect = 6.5e-61
Identity = 119/140 (85.00%), Postives = 131/140 (93.57%), Query Frame = 0

Query: 1   MASKATASVAFLLSLNLLFFSLVSACNNCYVPAPPKPKPCP---PVPKPTPSSGYGKCPR 60
           MASKAT S+AFLLSLNLLFF+LVSACNNCY+P PPKPKPCP   P PKP+PSSGYGKCPR
Sbjct: 1   MASKATTSLAFLLSLNLLFFTLVSACNNCYIPDPPKPKPCPPTKPTPKPSPSSGYGKCPR 60

Query: 61  DTLKLGVCAKLLGGLVDVTIGKPPVAPCCSLIEGLADLEAAVCLCTAIKANVLGNKLNIP 120
           DTLKLGVCAKLLGGL+D+TIGKPPV PCCSLIEGLADLEAAVCLCTAIKA++LG  LN+P
Sbjct: 61  DTLKLGVCAKLLGGLLDITIGKPPVTPCCSLIEGLADLEAAVCLCTAIKADILGINLNVP 120

Query: 121 VSLSLLLNVCKKNVPKGFKC 138
           +SLSLLLNVC+KNVPKGF+C
Sbjct: 121 LSLSLLLNVCQKNVPKGFQC 140

BLAST of Lag0032904 vs. ExPASy Swiss-Prot
Match: P14009 (14 kDa proline-rich protein DC2.15 OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 3.9e-40
Identity = 85/137 (62.04%), Postives = 101/137 (73.72%), Query Frame = 0

Query: 1   MASKATASVAFLLSLNLLFFSLVSACNNCYVPAPPKPKPCPPVPKPTPSSGYGKCPRDTL 60
           M SK +ASVA   +LN+LFF+LVS+   C  P  PKPKP P  P PTP    GKCPRD L
Sbjct: 1   MGSKNSASVALFFTLNILFFALVSSTEKCPDPYKPKPKPTPK-PTPTPYPSAGKCPRDAL 60

Query: 61  KLGVCAKLLGGLVDVTIGKPPVAPCCSLIEGLADLEAAVCLCTAIKANVLGNKLNIPVSL 120
           KLGVCA +L  + +V IG PP  PCCSL+EGL +LEAAVCLCTAIKAN+LG  LN+P++L
Sbjct: 61  KLGVCADVLNLVHNVVIGSPPTLPCCSLLEGLVNLEAAVCLCTAIKANILGKNLNLPIAL 120

Query: 121 SLLLNVCKKNVPKGFKC 138
           SL+LN C K VP GF+C
Sbjct: 121 SLVLNNCGKQVPNGFEC 136

BLAST of Lag0032904 vs. ExPASy Swiss-Prot
Match: Q9SU35 (pEARLI1-like lipid transfer protein 1 OS=Arabidopsis thaliana OX=3702 GN=AZI1 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 3.2e-34
Identity = 80/160 (50.00%), Postives = 105/160 (65.62%), Query Frame = 0

Query: 1   MASKATASVAFLLSLNLLFFSLVSACN-NCY-------VPAP-PKPKPCPPVPKP----- 60
           MASK +AS+A   +LN+LFF+L  A N NC        VP+P PKP  CPP P+P     
Sbjct: 1   MASKNSASLALFFALNILFFTLTVATNCNCKPSPKPKPVPSPKPKPVQCPPPPRPSVPSP 60

Query: 61  ---------TPSSGYGKCPRDTLKLGVCAKLLGGLVDVTIGKPPVAPCCSLIEGLADLEA 120
                    TP S    CP D LKLGVCA +L  L+++ +G+P    CCSLI+GL D++A
Sbjct: 61  NPRPVTPPRTPGSSGNSCPIDALKLGVCANVLSSLLNIQLGQPSSQQCCSLIQGLVDVDA 120

Query: 121 AVCLCTAIKANVLGNKLNIPVSLSLLLNVCKKNVPKGFKC 138
           A+CLCTA++ANVLG  LN+P+SLS+LLNVC + +P GF+C
Sbjct: 121 AICLCTALRANVLGINLNVPISLSVLLNVCNRKLPSGFQC 160

BLAST of Lag0032904 vs. ExPASy Swiss-Prot
Match: Q39176 (Lipid transfer protein EARLI 1 OS=Arabidopsis thaliana OX=3702 GN=EARLI1 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 7.1e-34
Identity = 81/167 (48.50%), Postives = 106/167 (63.47%), Query Frame = 0

Query: 1   MASKATASVAFLLSLNLLFFSLVSA----CNNC--YVPAP-PKPKPCP-PVPKPTPS--- 60
           MASK +AS+A   +LN++FF+L +A    CN    + P P PKPKP P P PKP PS   
Sbjct: 1   MASKNSASIALFFALNIIFFTLTAATDCGCNPSPKHKPVPSPKPKPVPSPKPKPVPSPSV 60

Query: 61  -------------------SGYGKCPRDTLKLGVCAKLLGGLVDVTIGKPPVAPCCSLIE 120
                                 G CP D L+LGVCA +L  L+++ +G+P   PCCSLI+
Sbjct: 61  PSPSVPSPNPRPVTPPRTPGSSGNCPIDALRLGVCANVLSSLLNIQLGQPSAQPCCSLIQ 120

Query: 121 GLADLEAAVCLCTAIKANVLGNKLNIPVSLSLLLNVCKKNVPKGFKC 138
           GL DL+AA+CLCTA++ANVLG  LN+P+SLS+LLNVC + VP GF+C
Sbjct: 121 GLVDLDAAICLCTALRANVLGINLNVPISLSVLLNVCNRKVPSGFQC 167

BLAST of Lag0032904 vs. ExPASy Swiss-Prot
Match: Q9SU34 (pEARLI1-like lipid transfer protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g12490 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 2.5e-31
Identity = 79/182 (43.41%), Postives = 103/182 (56.59%), Query Frame = 0

Query: 1   MASKATASVAFLLSLNLLFFSLVSACN----------------------------NCYVP 60
           MASK +AS+A   +LN+LFF+L +  N                            + YVP
Sbjct: 1   MASKNSASLALFFALNILFFTLTAGTNCRCNPSPKPRPLPNPKVPSPKVPTPSVPSPYVP 60

Query: 61  AP-----------------PKPKPCPPVPKPTPSSGYGKCPRDTLKLGVCAKLLGGLVDV 120
            P                 P P P P +P  TP S  G CP D L+LGVCA +L GL++V
Sbjct: 61  TPSVPSPSVPTPSVPSPSVPSPNPTPVIPPRTPGSS-GNCPIDALRLGVCANVLSGLLNV 120

Query: 121 TIGKPPVAPCCSLIEGLADLEAAVCLCTAIKANVLGNKLNIPVSLSLLLNVCKKNVPKGF 138
            +G+P   PCCSLI+GL DL+AAVCLCTA++ANVLG  LN+P+SLS+LLNVC + +P  F
Sbjct: 121 QLGQPSPQPCCSLIQGLVDLDAAVCLCTALRANVLGINLNVPISLSVLLNVCNRRLPSNF 180

BLAST of Lag0032904 vs. ExPASy Swiss-Prot
Match: Q9SU33 (pEARLI1-like lipid transfer protein 3 OS=Arabidopsis thaliana OX=3702 GN=At4g12500 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 9.6e-31
Identity = 77/176 (43.75%), Postives = 100/176 (56.82%), Query Frame = 0

Query: 1   MASKATASVAFLLSLNLLFFSLVSA----CN----------------------------- 60
           MASK + S+A   +LN+LFF+L +A    CN                             
Sbjct: 1   MASKNSTSLALFFALNILFFTLTTATDCRCNLSPKPRTVPSPKVPSPKYPSPSIPSPSVP 60

Query: 61  NCYVPAPPKPKPCPPVPKPTP------SSGYGKCPRDTLKLGVCAKLLGGLVDVTIGKPP 120
              VP P  P P  P P PTP          G CP D L+LGVCA +L GL++V +G+P 
Sbjct: 61  TPSVPTPSVPTPSVPSPNPTPVTPPRTPGSSGNCPIDALRLGVCANVLSGLLNVQLGQPS 120

Query: 121 VAPCCSLIEGLADLEAAVCLCTAIKANVLGNKLNIPVSLSLLLNVCKKNVPKGFKC 138
             PCCSLI+GL DL+AA+CLCTA++ANVLG  LN+P+SLS+LLNVC + +P  F+C
Sbjct: 121 AQPCCSLIQGLVDLDAAICLCTALRANVLGINLNVPISLSVLLNVCNRRLPSDFQC 176

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022922126.11.5e-6594.2414 kDa proline-rich protein DC2.15-like [Cucurbita moschata] >XP_023550963.1 14 ... [more]
XP_022970161.11.5e-6594.2014 kDa proline-rich protein DC2.15-like [Cucurbita maxima][more]
XP_038906980.11.7e-6491.9714 kDa proline-rich protein DC2.15-like [Benincasa hispida][more]
XP_008437101.12.0e-6289.29PREDICTED: 14 kDa proline-rich protein DC2.15-like [Cucumis melo] >KAA0039707.1 ... [more]
XP_004152410.26.5e-6185.0014 kDa proline-rich protein DC2.15 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
P140093.9e-4062.0414 kDa proline-rich protein DC2.15 OS=Daucus carota OX=4039 PE=2 SV=1[more]
Q9SU353.2e-3450.00pEARLI1-like lipid transfer protein 1 OS=Arabidopsis thaliana OX=3702 GN=AZI1 PE... [more]
Q391767.1e-3448.50Lipid transfer protein EARLI 1 OS=Arabidopsis thaliana OX=3702 GN=EARLI1 PE=1 SV... [more]
Q9SU342.5e-3143.41pEARLI1-like lipid transfer protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g124... [more]
Q9SU339.6e-3143.75pEARLI1-like lipid transfer protein 3 OS=Arabidopsis thaliana OX=3702 GN=At4g125... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainSMARTSM00499aai_6coord: 55..137
e-value: 5.9E-9
score: 45.8
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilyGENE3D1.10.110.10coord: 54..137
e-value: 1.0E-28
score: 101.0
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilySUPERFAMILY47699Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumincoord: 57..137
IPR027923Hydrophobic seed protein domainPFAMPF14547Hydrophob_seedcoord: 54..137
e-value: 7.2E-27
score: 93.4
IPR027923Hydrophobic seed protein domainCDDcd01958HPS_likecoord: 53..137
e-value: 1.52899E-28
score: 98.1696
NoneNo IPR availablePANTHERPTHR31731FAMILY NOT NAMEDcoord: 5..137
NoneNo IPR availablePANTHERPTHR31731:SF87BNAA08G24920D PROTEINcoord: 5..137
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..26
score: 5.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032904.1Lag0032904.1mRNA