Lag0032837 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0032837
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	CCHC-type domain-containing protein
Location	chr11: 38125194 .. 38126366 (-)
RNA-Seq Expression	Lag0032837
Synteny	Lag0032837

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAACTCCCTGCGAATTCAAGTTAAGATTGATGTAACAGAACCACTCACCAGAGGCCTGCTGGTCCAAGTTGGGTCCAAAGGAGAGGAAGTGTGGATGAAAATCACCTACGAACGCCTCCCGGACTTCTGCTACAAATGTGGGAGAATCGGTCACACACTAAAAGAATGCGAAGAGATAATCAACCCGGAAGAAGAGGGCCTTTTTGGAGACTGGATGAGAGCAGCACCGATAGGGATGGGAACACCAAACCAAGGCAACCAAGAACAGAGAAGGGAATATAGTTGGGGCAGAGGCAGGGGAAGAAGAGGGGGCAACTCAAGATCCCATCATCAACAACAAAGGGACTGGTTTGATCGCAGAAAAAATGACTCGAAGAAAGAAGAAAGAAACCAAACTCACCAGCCAACCCTAGCAATGGAGGTGGATGAACCAATAAGTATAGAAACAGGGCCAAAGCAGACTCAACGAGAAGAGGGAAAAACAAACATCTCACAAAGCTCAACCTCGCCGGAAAACGGGGAGGAAGATGACCGGAATAGTGAGGAGGTCAGGCAACGCCGGTGGACGAACAAAACGGGAACAGAAATCGAGGAAGCTAGGGAGGAGTGCCAGAACAACAAAGTGGTGTCAGAAGAAGCAGGGAAAATAGCCGTTGGAATAGAAAACAGTGAAAATACCCAAAAGGAAGAAGTGTGTAAGAAGGAAAAAGAGAAAAATAGGAATATTCTAAGCGGTGACAGGTCAAAAGACAATATGACTACTAAGGTAGGAAAGTGGGACCCAGGATCACGTGTTTCCAAGTTAGAAAATCAAAAGGAAAACAAAGCCCAGCAGGAAACAGGAGAAAAGGCCCACCAACAAACCTCCAGATATGAAGAAAAGTCCCACCAACAAACCTCCAGATCTGAAGAAAAAGAAAGCTTTTCTCCAAAGGACAGAGGGAAATTCGAAACAAAAAACAAACTATCCAGGAAATGGAAGAGGCTCGCGCGGGCTGATCCAAAAAGTCAAAATAGAGTCGAGAACAATGAAATTCACAAGAGGAAAATGGAAACTACCTGTGAGGAGGAGTCAAGGGAGGAGACAAAGAGGAAGCGAACAACGTACTTCGAAGTAGTCGAAGGGATATCGGCGGAGGCTGGATACCAGCCCCACCGGATGCAATGA

mRNA sequence

ATGGGAAACTCCCTGCGAATTCAAGTTAAGATTGATGTAACAGAACCACTCACCAGAGGCCTGCTGGTCCAAGTTGGGTCCAAAGGAGAGGAAGTGTGGATGAAAATCACCTACGAACGCCTCCCGGACTTCTGCTACAAATGTGGGAGAATCGGTCACACACTAAAAGAATGCGAAGAGATAATCAACCCGGAAGAAGAGGGCCTTTTTGGAGACTGGATGAGAGCAGCACCGATAGGGATGGGAACACCAAACCAAGGCAACCAAGAACAGAGAAGGGAATATAGTTGGGGCAGAGGCAGGGGAAGAAGAGGGGGCAACTCAAGATCCCATCATCAACAACAAAGGGACTGGTTTGATCGCAGAAAAAATGACTCGAAGAAAGAAGAAAGAAACCAAACTCACCAGCCAACCCTAGCAATGGAGGTGGATGAACCAATAAGTATAGAAACAGGGCCAAAGCAGACTCAACGAGAAGAGGGAAAAACAAACATCTCACAAAGCTCAACCTCGCCGGAAAACGGGGAGGAAGATGACCGGAATAGTGAGGAGGTCAGGCAACGCCGGTGGACGAACAAAACGGGAACAGAAATCGAGGAAGCTAGGGAGGAGTGCCAGAACAACAAAGTGGTGTCAGAAGAAGCAGGGAAAATAGCCGTTGGAATAGAAAACAGTGAAAATACCCAAAAGGAAGAAGTGTGTAAGAAGGAAAAAGAGAAAAATAGGAATATTCTAAGCGGTGACAGGTCAAAAGACAATATGACTACTAAGGTAGGAAAGTGGGACCCAGGATCACGTGTTTCCAAGTTAGAAAATCAAAAGGAAAACAAAGCCCAGCAGGAAACAGGAGAAAAGGCCCACCAACAAACCTCCAGATATGAAGAAAAGTCCCACCAACAAACCTCCAGATCTGAAGAAAAAGAAAGCTTTTCTCCAAAGGACAGAGGGAAATTCGAAACAAAAAACAAACTATCCAGGAAATGGAAGAGGCTCGCGCGGGCTGATCCAAAAAGTCAAAATAGAGTCGAGAACAATGAAATTCACAAGAGGAAAATGGAAACTACCTGTGAGGAGGAGTCAAGGGAGGAGACAAAGAGGAAGCGAACAACGTACTTCGAAGTAGTCGAAGGGATATCGGCGGAGGCTGGATACCAGCCCCACCGGATGCAATGA

Coding sequence (CDS)

ATGGGAAACTCCCTGCGAATTCAAGTTAAGATTGATGTAACAGAACCACTCACCAGAGGCCTGCTGGTCCAAGTTGGGTCCAAAGGAGAGGAAGTGTGGATGAAAATCACCTACGAACGCCTCCCGGACTTCTGCTACAAATGTGGGAGAATCGGTCACACACTAAAAGAATGCGAAGAGATAATCAACCCGGAAGAAGAGGGCCTTTTTGGAGACTGGATGAGAGCAGCACCGATAGGGATGGGAACACCAAACCAAGGCAACCAAGAACAGAGAAGGGAATATAGTTGGGGCAGAGGCAGGGGAAGAAGAGGGGGCAACTCAAGATCCCATCATCAACAACAAAGGGACTGGTTTGATCGCAGAAAAAATGACTCGAAGAAAGAAGAAAGAAACCAAACTCACCAGCCAACCCTAGCAATGGAGGTGGATGAACCAATAAGTATAGAAACAGGGCCAAAGCAGACTCAACGAGAAGAGGGAAAAACAAACATCTCACAAAGCTCAACCTCGCCGGAAAACGGGGAGGAAGATGACCGGAATAGTGAGGAGGTCAGGCAACGCCGGTGGACGAACAAAACGGGAACAGAAATCGAGGAAGCTAGGGAGGAGTGCCAGAACAACAAAGTGGTGTCAGAAGAAGCAGGGAAAATAGCCGTTGGAATAGAAAACAGTGAAAATACCCAAAAGGAAGAAGTGTGTAAGAAGGAAAAAGAGAAAAATAGGAATATTCTAAGCGGTGACAGGTCAAAAGACAATATGACTACTAAGGTAGGAAAGTGGGACCCAGGATCACGTGTTTCCAAGTTAGAAAATCAAAAGGAAAACAAAGCCCAGCAGGAAACAGGAGAAAAGGCCCACCAACAAACCTCCAGATATGAAGAAAAGTCCCACCAACAAACCTCCAGATCTGAAGAAAAAGAAAGCTTTTCTCCAAAGGACAGAGGGAAATTCGAAACAAAAAACAAACTATCCAGGAAATGGAAGAGGCTCGCGCGGGCTGATCCAAAAAGTCAAAATAGAGTCGAGAACAATGAAATTCACAAGAGGAAAATGGAAACTACCTGTGAGGAGGAGTCAAGGGAGGAGACAAAGAGGAAGCGAACAACGTACTTCGAAGTAGTCGAAGGGATATCGGCGGAGGCTGGATACCAGCCCCACCGGATGCAATGA

Protein sequence

MGNSLRIQVKIDVTEPLTRGLLVQVGSKGEEVWMKITYERLPDFCYKCGRIGHTLKECEEIINPEEEGLFGDWMRAAPIGMGTPNQGNQEQRREYSWGRGRGRRGGNSRSHHQQQRDWFDRRKNDSKKEERNQTHQPTLAMEVDEPISIETGPKQTQREEGKTNISQSSTSPENGEEDDRNSEEVRQRRWTNKTGTEIEEAREECQNNKVVSEEAGKIAVGIENSENTQKEEVCKKEKEKNRNILSGDRSKDNMTTKVGKWDPGSRVSKLENQKENKAQQETGEKAHQQTSRYEEKSHQQTSRSEEKESFSPKDRGKFETKNKLSRKWKRLARADPKSQNRVENNEIHKRKMETTCEEESREETKRKRTTYFEVVEGISAEAGYQPHRMQ

Homology

[Close]

BLAST of Lag0032837 vs. NCBI nr
Match: KAE8772589.1 (hypothetical protein D1007_55405 [Hordeum vulgare])

HSP 1 Score: 90.9 bits (224), Expect = 2.6e-14
Identity = 40/101 (39.60%), Postives = 59/101 (58.42%), Query Frame = 0


Query: 1   MGNSLRIQVKIDVTEPLTRGLLVQVGSKGEEVWMKITYERLPDFCYKCGRIGHTLKECEE 60
           +G  LR++VK+++T+PL RG +VQ+  +G + W    YE LP+FCY CG I H  +EC  
Sbjct: 163 VGKYLRVKVKLNITKPLMRGSMVQIDERGRKSWCPFAYEYLPNFCYTCGVISHVERECNT 222

Query: 61  IINPEEEGLFGDWMRAAPIGMGTPNQGNQEQRREYSWGRGR 102
            +  +E+  FG W+RA   G  T ++G        S G GR
Sbjct: 223 KVKKDEKAQFGRWLRAEVEGRRTEDRGRSSGSGSTSSGGGR 263

[Close]

BLAST of Lag0032837 vs. NCBI nr
Match: PNX56720.1 (hypothetical protein L195_g058346 [Trifolium pratense])

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-12
Identity = 47/106 (44.34%), Postives = 64/106 (60.38%), Query Frame = 0


Query: 2   GNSLRIQVKIDVTEPLTRGLLVQVGSKGEEVWMKITYERLPDFCYKCGRIGHTLKECEEI 61
           G  LR++V ID+T+PL RG +++   +  +V+ K  YERLP FC+ CGRIGH LKECEE 
Sbjct: 24  GRFLRLKVSIDLTKPLKRGTVIKYQDRSLKVFFK--YERLPTFCFVCGRIGHQLKECEEA 83

Query: 62  INPEEEGL---------FGDWMRAAPIGMGTPNQGNQEQRREYSWG 99
            + EE G          FG W+RA+P+      + N E R+E S G
Sbjct: 84  GDLEEAGYEELDEKELGFGPWLRASPL-----PKFNFEPRKESSSG 122

[Close]

BLAST of Lag0032837 vs. NCBI nr
Match: PNY15174.1 (ribonuclease H, partial [Trifolium pratense])

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-12
Identity = 38/88 (43.18%), Postives = 56/88 (63.64%), Query Frame = 0


Query: 1   MGNSLRIQVKIDVTEPLTRGLLVQVGSKGEEVWMKITYERLPDFCYKCGRIGHTLKECEE 60
           +G  LR++V ID+ +PL RG ++    K   V+ K  YERLP+FCY CGRIGH +K+CEE
Sbjct: 245 LGKFLRVKVSIDLRKPLKRGTVLNYQGKRLHVFFK--YERLPNFCYMCGRIGHQMKDCEE 304

Query: 61  IINPEEEGL---------FGDWMRAAPI 80
           +   EE+G          +G W++A+P+
Sbjct: 305 VEGNEEDGYEDIEEKELPYGSWLKASPL 330

[Close]

BLAST of Lag0032837 vs. NCBI nr
Match: GAU30026.1 (hypothetical protein TSUD_161120 [Trifolium subterraneum])

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-12
Identity = 42/92 (45.65%), Postives = 56/92 (60.87%), Query Frame = 0


Query: 1   MGNSLRIQVKIDVTEPLTRGLLVQVGSKGEEVWMKITYERLPDFCYKCGRIGHTLKECEE 60
           MG  LR +V ID+T+PL RG +V+   K  +V+ K  YERLP  C+ CGRIGH L++C+E
Sbjct: 158 MGRFLRAKVTIDLTKPLKRGTIVRYNGKDLKVFFK--YERLPTLCFLCGRIGHQLRDCDE 217

Query: 61  IINPEEEGL---------FGDWMRAAPIGMGT 84
           I   E EG          FG W+RA+P+   T
Sbjct: 218 IGGNEGEGFEEIEEKELPFGPWLRASPLPRNT 247

[Close]

BLAST of Lag0032837 vs. NCBI nr
Match: KAG0532780.1 (hypothetical protein BDA96_04G136500, partial [Sorghum bicolor])

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-12
Identity = 43/99 (43.43%), Postives = 60/99 (60.61%), Query Frame = 0


Query: 5   LRIQVKIDVTEPLTRGLLVQVGSKGEEVWMKITYERLPDFCYKCGRIGHTLKECEEIINP 64
           LRI+VKID+ +PL RG+LV+     E+ W  I YE +P+FCY CG +GH  KEC+  +  
Sbjct: 9   LRIKVKIDIRKPLRRGILVEGEDGEEDCWCPIKYEFIPNFCYSCGCLGHVEKECDSCVEL 68

Query: 65  EEE-GLFGDWMRAAPIGMGTPNQGNQEQRREYSWGRGRG 103
           E     FG+WMR  P    +  +G  +QR ++S GR  G
Sbjct: 69  EAGCRQFGEWMRVTP----SRWKGQGDQRNKWSEGRNSG 103

[Close]

BLAST of Lag0032837 vs. ExPASy TrEMBL
Match: A0A803P119 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-13
Identity = 37/91 (40.66%), Postives = 57/91 (62.64%), Query Frame = 0


Query: 2   GNSLRIQVKIDVTEPLTRGLLVQVGSKGEEVWMKITYERLPDFCYKCGRIGHTLKECEEI 61
           G  +RI++ +DVT+PL RG L+++    +++W+ + YE+LPD CYKCGR+GHT   C E 
Sbjct: 495 GPFMRIRISLDVTQPLLRGTLLKITGLVDDLWVILKYEKLPDICYKCGRLGHTFLRCIEF 554

Query: 62  INPEEEG-----LFGDWMRAAPI---GMGTP 85
           +  E+ G      +G WM+  P+   G G P
Sbjct: 555 LEAEDVGGDTSLPYGPWMKGDPLPRRGTGRP 585

[Close]

BLAST of Lag0032837 vs. ExPASy TrEMBL
Match: A0A2Z6MBL0 (Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_161120 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 5.4e-13
Identity = 42/92 (45.65%), Postives = 56/92 (60.87%), Query Frame = 0


Query: 1   MGNSLRIQVKIDVTEPLTRGLLVQVGSKGEEVWMKITYERLPDFCYKCGRIGHTLKECEE 60
           MG  LR +V ID+T+PL RG +V+   K  +V+ K  YERLP  C+ CGRIGH L++C+E
Sbjct: 158 MGRFLRAKVTIDLTKPLKRGTIVRYNGKDLKVFFK--YERLPTLCFLCGRIGHQLRDCDE 217

Query: 61  IINPEEEGL---------FGDWMRAAPIGMGT 84
           I   E EG          FG W+RA+P+   T
Sbjct: 218 IGGNEGEGFEEIEEKELPFGPWLRASPLPRNT 247

[Close]

BLAST of Lag0032837 vs. ExPASy TrEMBL
Match: A0A2K3PIT6 (Ribonuclease H (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g011865 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 5.4e-13
Identity = 38/88 (43.18%), Postives = 56/88 (63.64%), Query Frame = 0


Query: 1   MGNSLRIQVKIDVTEPLTRGLLVQVGSKGEEVWMKITYERLPDFCYKCGRIGHTLKECEE 60
           +G  LR++V ID+ +PL RG ++    K   V+ K  YERLP+FCY CGRIGH +K+CEE
Sbjct: 245 LGKFLRVKVSIDLRKPLKRGTVLNYQGKRLHVFFK--YERLPNFCYMCGRIGHQMKDCEE 304

Query: 61  IINPEEEGL---------FGDWMRAAPI 80
           +   EE+G          +G W++A+P+
Sbjct: 305 VEGNEEDGYEDIEEKELPYGSWLKASPL 330

[Close]

BLAST of Lag0032837 vs. ExPASy TrEMBL
Match: A0A2K3JRR7 (CCHC-type domain-containing protein OS=Trifolium pratense OX=57577 GN=L195_g058346 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 5.4e-13
Identity = 47/106 (44.34%), Postives = 64/106 (60.38%), Query Frame = 0


Query: 2   GNSLRIQVKIDVTEPLTRGLLVQVGSKGEEVWMKITYERLPDFCYKCGRIGHTLKECEEI 61
           G  LR++V ID+T+PL RG +++   +  +V+ K  YERLP FC+ CGRIGH LKECEE 
Sbjct: 24  GRFLRLKVSIDLTKPLKRGTVIKYQDRSLKVFFK--YERLPTFCFVCGRIGHQLKECEEA 83

Query: 62  INPEEEGL---------FGDWMRAAPIGMGTPNQGNQEQRREYSWG 99
            + EE G          FG W+RA+P+      + N E R+E S G
Sbjct: 84  GDLEEAGYEELDEKELGFGPWLRASPL-----PKFNFEPRKESSSG 122

[Close]

BLAST of Lag0032837 vs. ExPASy TrEMBL
Match: A0A803PVI8 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 9.2e-13
Identity = 38/101 (37.62%), Postives = 59/101 (58.42%), Query Frame = 0


Query: 2   GNSLRIQVKIDVTEPLTRGLLVQVGSKGEEVWMKITYERLPDFCYKCGRIGHTLKECEEI 61
           G  LR++V+IDV++PL RG  V      +E+W+   YERLPDFCY+CG IGH   +C   
Sbjct: 165 GPFLRMRVEIDVSKPLLRGQFVTFPWMADELWLDYRYERLPDFCYECGIIGHVFDKCPLF 224

Query: 62  INPEEEG-----LFGDWMRAAPIGMGTPNQGNQEQRREYSW 98
           +   ++G     L+G WM  +P+   + ++  Q+  +E  W
Sbjct: 225 LEKIDDGVEPSLLYGPWMEGSPLPRSSYDRYRQDFSKEGPW 265

The following BLAST results are available for this feature:

BLAST of Lag0032837 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8772589.1	2.6e-14	39.60	hypothetical protein D1007_55405 [Hordeum vulgare]	[more]
PNX56720.1	1.1e-12	44.34	hypothetical protein L195_g058346 [Trifolium pratense]	[more]
PNY15174.1	1.1e-12	43.18	ribonuclease H, partial [Trifolium pratense]	[more]
GAU30026.1	1.1e-12	45.65	hypothetical protein TSUD_161120 [Trifolium subterraneum]	[more]
KAG0532780.1	1.1e-12	43.43	hypothetical protein BDA96_04G136500, partial [Sorghum bicolor]	[more]

BLAST of Lag0032837 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0032837 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A803P119	1.1e-13	40.66	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A2Z6MBL0	5.4e-13	45.65	Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_161120 PE=4 SV...	[more]
A0A2K3PIT6	5.4e-13	43.18	Ribonuclease H (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g011865 PE=4 SV...	[more]
A0A2K3JRR7	5.4e-13	44.34	CCHC-type domain-containing protein OS=Trifolium pratense OX=57577 GN=L195_g0583...	[more]
A0A803PVI8	9.2e-13	37.62	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

BLAST of Lag0032837 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025836	Zinc knuckle CX2CX4HX4C	PFAM	PF14392	zf-CCHC_4	coord: 11..59 e-value: 1.4E-13 score: 50.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 329..367
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 150..172
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 173..210
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 76..367
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 110..135
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 267..321
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 228..247
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 45..60 score: 10.114237

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0032837.1	Lag0032837.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding