Lag0032831 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032831
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr11: 38076979 .. 38077626 (+)
RNA-Seq ExpressionLag0032831
SyntenyLag0032831
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGTCGACCCGAAATAAAGTTCGTCTTCAAGGGGCTGATAGGCCGAGTGGGCTAGTGGAATGGGCGAAGGGGTATGTGGTGGCTTTTCGTGAGGTTGGGAGGAGTAGTGGGGAAGTGGGGCGGGAGACGGTTGGTCGGGAGAGGGTAGTGTGGCGGGCCCCGAGGAATGGTTGGTATAAGGTGAATTTTGATGCCTCTTTCCTTTCTCATTTGTCCAGGGCCGGCCTAGGGATAATTGTGCGGGATCCTTTGGGCCAAGTAATGCTGTCGGCGACCTTTACTAAGGATAATGTGAGAGATGTTGACATGGCTGAAGGATATGCGGCCGTGAAAAGTTTGGAGTTGGTGACAGATATGGGTTTGGGTCCAGCAATTCTTGAGACGGATTCGAGTAGAGTATTCCAGATCCTTAATCGAGAACGTGAGGATATTTCTGAGCTAGGTATGATTATTGCAGATGCACTAATGGCTTTTCCTTCTAATTTGTAGGTACTGTTTCATTTCGTTCACCGTGAAGGGAATCAGGCGACGCACCGATTAGCGAGGTTAGCAATAGCCCAGTGGAGTGATGAGGTTTGGATGGAGACTTGTCCTAGGTATCTGGAGGACATAGTTTTGAACGATTCAATGTACATGGAGGCATGA

mRNA sequence

ATGTGGTCGACCCGAAATAAAGTTCGTCTTCAAGGGGCTGATAGGCCGAGTGGGCTAGTGGAATGGGCGAAGGGGTATGTGGTGGCTTTTCGTGAGGTTGGGAGGAGTAGTGGGGAAGTGGGGCGGGAGACGGTTGGTCGGGAGAGGGTAGTGTGGCGGGCCCCGAGGAATGGTTGGTATAAGGTGAATTTTGATGCCTCTTTCCTTTCTCATTTGTCCAGGGCCGGCCTAGGGATAATTGTGCGGGATCCTTTGGGCCAAGTAATGCTGTCGGCGACCTTTACTAAGGATAATGTGAGAGATGTTGACATGGCTGAAGGATATGCGGCCGTGAAAAGTTTGGAGTTGGTGACAGATATGGGTTTGGGTCCAGCAATTCTTGAGACGGATTCGAGTAGAGTATTCCAGATCCTTAATCGAGAACGTGAGGATATTTCTGAGCTAGGGAATCAGGCGACGCACCGATTAGCGAGGTTAGCAATAGCCCAGTGGAGTGATGAGGTTTGGATGGAGACTTGTCCTAGGTATCTGGAGGACATAGTTTTGAACGATTCAATGTACATGGAGGCATGA

Coding sequence (CDS)

ATGTGGTCGACCCGAAATAAAGTTCGTCTTCAAGGGGCTGATAGGCCGAGTGGGCTAGTGGAATGGGCGAAGGGGTATGTGGTGGCTTTTCGTGAGGTTGGGAGGAGTAGTGGGGAAGTGGGGCGGGAGACGGTTGGTCGGGAGAGGGTAGTGTGGCGGGCCCCGAGGAATGGTTGGTATAAGGTGAATTTTGATGCCTCTTTCCTTTCTCATTTGTCCAGGGCCGGCCTAGGGATAATTGTGCGGGATCCTTTGGGCCAAGTAATGCTGTCGGCGACCTTTACTAAGGATAATGTGAGAGATGTTGACATGGCTGAAGGATATGCGGCCGTGAAAAGTTTGGAGTTGGTGACAGATATGGGTTTGGGTCCAGCAATTCTTGAGACGGATTCGAGTAGAGTATTCCAGATCCTTAATCGAGAACGTGAGGATATTTCTGAGCTAGGGAATCAGGCGACGCACCGATTAGCGAGGTTAGCAATAGCCCAGTGGAGTGATGAGGTTTGGATGGAGACTTGTCCTAGGTATCTGGAGGACATAGTTTTGAACGATTCAATGTACATGGAGGCATGA

Protein sequence

MWSTRNKVRLQGADRPSGLVEWAKGYVVAFREVGRSSGEVGRETVGRERVVWRAPRNGWYKVNFDASFLSHLSRAGLGIIVRDPLGQVMLSATFTKDNVRDVDMAEGYAAVKSLELVTDMGLGPAILETDSSRVFQILNREREDISELGNQATHRLARLAIAQWSDEVWMETCPRYLEDIVLNDSMYMEA
Homology
BLAST of Lag0032831 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 128.3 bits (321), Expect = 7.3e-26
Identity = 78/185 (42.16%), Postives = 102/185 (55.14%), Query Frame = 0

Query: 19  LVEWAKGYVVAFREVGRSSGEVGRETVGRERVVWRAPRNGWYKVNFDASFLSHLSRAGLG 78
           LVEWA  YV+ FRE   S+   GR T   E V+W  P    YK+N DASFL+    AGLG
Sbjct: 3   LVEWANKYVMEFRE-ANSNPFPGRVTNTAE-VLWLPPDKRIYKINTDASFLASDQHAGLG 62

Query: 79  IIVRDPLGQVMLSATFTKDNVRDVDMAEGYAAVKSLELVTDMGLGPAILETDSSRVFQIL 138
           II+R+  GQVM SAT   +N++ VDMAE   AV+ L+L + +G+ P ILETDSSR+F + 
Sbjct: 63  IIIRNDRGQVMASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNLF 122

Query: 139 NREREDISEL-------------------------GNQATHRLARLAIAQWSDEVWMETC 179
           ++  ED+SE                          GN+A H LAR A+      +WME  
Sbjct: 123 SQPSEDLSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLREFSIWMEDW 182

BLAST of Lag0032831 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 112.1 bits (279), Expect = 5.4e-21
Identity = 66/169 (39.05%), Postives = 95/169 (56.21%), Query Frame = 0

Query: 19   LVEWAKGYVVAFREVGRSSGEVGRETVGRERVVWRAPRNGWYKVNFDASFLSHLSRAGLG 78
            LVEWA  Y + FRE  +S+   GR T   E ++W+ P  G YK+N DASFL+    AGLG
Sbjct: 960  LVEWANKYAMEFRE-AKSNPITGRVTNTAE-ILWQPPDEGIYKINTDASFLASDQHAGLG 1019

Query: 79   IIVRDPLGQVMLSATFTKDNVRDVDMAEGYAAVKSLELVTDMGLGPAILETDSSRVF--- 138
            II+ +  GQVM +AT   +N++ VDMAE  AAV+ L+L +++G+ PA+ +   +      
Sbjct: 1020 IIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIGMHPALEDLSETGEIVLK 1079

Query: 139  ------QILNREREDISELGNQATHRLARLAIAQWSDEVWMETCPRYLE 179
                  Q L+     +   GN+A H LAR A+      +WME  P  L+
Sbjct: 1080 AKNFWTQSLHASFNFVKREGNKAAHMLARRALLLHEFSIWMEDWPLELK 1126

BLAST of Lag0032831 vs. NCBI nr
Match: XP_028115288.1 (uncharacterized protein LOC114313136 isoform X3 [Camellia sinensis])

HSP 1 Score: 98.6 bits (244), Expect = 6.2e-17
Identity = 58/160 (36.25%), Postives = 78/160 (48.75%), Query Frame = 0

Query: 50  VVWRAPRNGWYKVNFDASFLSHLSRAGLGIIVRDPLGQVMLSATFTKDNVRDVDMAEGYA 109
           V W AP NGW K+NFD +    L   G+G+++R+ LG+VM + +       D D AE YA
Sbjct: 657 VGWCAPANGWLKINFDGALFKELKAVGVGVVIRNHLGEVMAALSERLPFWVDSDCAEAYA 716

Query: 110 AVKSLELVTDMGLGPAILETDSSRVFQILNREREDISEL--------------------- 169
           A K++EL  D+GL    LE DS R+ ++L  E E +SE                      
Sbjct: 717 ATKAVELARDLGLSDIHLEGDSLRIVKVLREEAEFMSEYGHILLQTVGVCKSFSRFHVSH 776

Query: 170 ----GNQATHRLARLAIAQWSDEVWMETCPRYLEDIVLND 185
               GN   H LAR+A  Q   +VWME  P  L  I  +D
Sbjct: 777 VGRQGNGLAHGLARMARHQNYHQVWMEGLPASLIAIATSD 816

BLAST of Lag0032831 vs. NCBI nr
Match: XP_028115297.1 (uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis] >XP_028115298.1 uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis] >XP_028115299.1 uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis] >XP_028115300.1 uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis] >XP_028115301.1 uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis])

HSP 1 Score: 98.6 bits (244), Expect = 6.2e-17
Identity = 58/160 (36.25%), Postives = 78/160 (48.75%), Query Frame = 0

Query: 50  VVWRAPRNGWYKVNFDASFLSHLSRAGLGIIVRDPLGQVMLSATFTKDNVRDVDMAEGYA 109
           V W AP NGW K+NFD +    L   G+G+++R+ LG+VM + +       D D AE YA
Sbjct: 667 VGWCAPANGWLKINFDGALFKELKAVGVGVVIRNHLGEVMAALSERLPFWVDSDCAEAYA 726

Query: 110 AVKSLELVTDMGLGPAILETDSSRVFQILNREREDISEL--------------------- 169
           A K++EL  D+GL    LE DS R+ ++L  E E +SE                      
Sbjct: 727 ATKAVELARDLGLSDIHLEGDSLRIVKVLREEAEFMSEYGHILLQTVGVCKSFSRFHVSH 786

Query: 170 ----GNQATHRLARLAIAQWSDEVWMETCPRYLEDIVLND 185
               GN   H LAR+A  Q   +VWME  P  L  I  +D
Sbjct: 787 VGRQGNGLAHGLARMARHQNYHQVWMEGLPASLIAIATSD 826

BLAST of Lag0032831 vs. NCBI nr
Match: XP_028115286.1 (uncharacterized protein LOC114313136 isoform X1 [Camellia sinensis])

HSP 1 Score: 98.6 bits (244), Expect = 6.2e-17
Identity = 58/160 (36.25%), Postives = 78/160 (48.75%), Query Frame = 0

Query: 50  VVWRAPRNGWYKVNFDASFLSHLSRAGLGIIVRDPLGQVMLSATFTKDNVRDVDMAEGYA 109
           V W AP NGW K+NFD +    L   G+G+++R+ LG+VM + +       D D AE YA
Sbjct: 700 VGWCAPANGWLKINFDGALFKELKAVGVGVVIRNHLGEVMAALSERLPFWVDSDCAEAYA 759

Query: 110 AVKSLELVTDMGLGPAILETDSSRVFQILNREREDISEL--------------------- 169
           A K++EL  D+GL    LE DS R+ ++L  E E +SE                      
Sbjct: 760 ATKAVELARDLGLSDIHLEGDSLRIVKVLREEAEFMSEYGHILLQTVGVCKSFSRFHVSH 819

Query: 170 ----GNQATHRLARLAIAQWSDEVWMETCPRYLEDIVLND 185
               GN   H LAR+A  Q   +VWME  P  L  I  +D
Sbjct: 820 VGRQGNGLAHGLARMARHQNYHQVWMEGLPASLIAIATSD 859

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140628.17.3e-2642.16uncharacterized protein LOC111011237 [Momordica charantia][more]
XP_022150918.15.4e-2139.05uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_028115288.16.2e-1736.25uncharacterized protein LOC114313136 isoform X3 [Camellia sinensis][more]
XP_028115297.16.2e-1736.25uncharacterized protein LOC114313136 isoform X6 [Camellia sinensis] >XP_02811529... [more]
XP_028115286.16.2e-1736.25uncharacterized protein LOC114313136 isoform X1 [Camellia sinensis][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 63..152
e-value: 1.0E-12
score: 48.0
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 59..171
e-value: 7.8E-8
score: 34.4
NoneNo IPR availablePANTHERPTHR47074:SF25SUBFAMILY NOT NAMEDcoord: 49..151
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 49..151
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 58..164

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032831.1Lag0032831.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity