Lag0032769 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0032769 vs. NCBI nr
Match: XP_022159636.1 (GDSL esterase/lipase 1-like [Momordica charantia])

HSP 1 Score: 617.1 bits (1590), Expect = 9.9e-173
Identity = 294/363 (80.99%), Postives = 322/363 (88.71%), Query Frame = 0

Query: 4   SKLLSCVFLAILAISSLSPHSCEPWPKQELRPLFVFGDSLYDVGNNNYINTIAIANFPPY 63
           SKL+SCV L ILA+S LSPHSCEP P  E  PLFVFGDSLYDVG NNYINT A ANFPPY
Sbjct: 5   SKLVSCVVLGILAMSCLSPHSCEPEP--EPVPLFVFGDSLYDVGINNYINTSAQANFPPY 64

Query: 64  GETFFKFPSGRFSDGREIPDFIAEYANLPMILPYLYPGIKDFVKGVNFASGGAGALDETF 123
           GETFFKFP+GRFSDGR IPDFIAEYANLP+ILPYLYPGIKD+VKGVNFASGGAGALD+TF
Sbjct: 65  GETFFKFPTGRFSDGRVIPDFIAEYANLPLILPYLYPGIKDYVKGVNFASGGAGALDQTF 124

Query: 124 PESVVTFRAQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSNDYGAFDPQSKVYQS 183
           P+  VT R Q+G+FKEME  LR  LG SETKKLLSRAVYLIAIG NDYGAFD    VYQS
Sbjct: 125 PQLAVTLRTQLGYFKEMETKLRNKLGTSETKKLLSRAVYLIAIGGNDYGAFDSNFTVYQS 184

Query: 184 YTTEQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEALIYHGKVDWVEQFK 243
           YTTEQYVDSVIGNM+  I+ IYKMGGRKFSVLNVGP+DRLP M+EA IYHG+VDWVEQ +
Sbjct: 185 YTTEQYVDSVIGNMSSVIKGIYKMGGRKFSVLNVGPMDRLPAMREAAIYHGRVDWVEQVE 244

Query: 244 QFALLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKYGMKEVKSGCCGIGPL 303
            FA+LHN++LPKALQKL +KLKGF+YSH DFYTALN+IID+P+KYG+KEVKSGCCGIGPL
Sbjct: 245 NFAVLHNQQLPKALQKLQQKLKGFIYSHTDFYTALNDIIDNPTKYGIKEVKSGCCGIGPL 304

Query: 304 RGNNTCGGKRGIKEYELCGNPEEYVFFDATHLTDRAYHLIADMMWNGTSKITSPLNVKSL 363
           RG N+CGGKRGIKEYELCGNPEEYVFFDATHLT RAY +IA +MW GTS IT+PLNVKSL
Sbjct: 305 RGTNSCGGKRGIKEYELCGNPEEYVFFDATHLTHRAYQIIAQIMWTGTSNITTPLNVKSL 364

Query: 364 FYI 367
           FYI
Sbjct: 365 FYI 365

BLAST of Lag0032769 vs. NCBI nr
Match: XP_022159717.1 (GDSL esterase/lipase 1-like [Momordica charantia])

HSP 1 Score: 594.0 bits (1530), Expect = 9.0e-166
Identity = 284/365 (77.81%), Postives = 315/365 (86.30%), Query Frame = 0

Query: 4   SKLLSCVFLAILAISSLSPHSCEPWPKQELRPLFVFGDSLYDVGNNNYINTI--AIANFP 63
           SKL+SCV L ILA+  LSPHSC   P     PLFVFGDSLYDVGNNNYINT   A ANFP
Sbjct: 5   SKLVSCVILGILAMRCLSPHSCHLEPV----PLFVFGDSLYDVGNNNYINTTAGAQANFP 64

Query: 64  PYGETFFKFPSGRFSDGREIPDFIAEYANLPMILPYLYPGIKDFVKGVNFASGGAGALDE 123
           PYGETFFKFP+GRF DGR IPDFIAE ANLP+ILPYLYPGIKD VKGVNFASGG+GALD 
Sbjct: 65  PYGETFFKFPTGRFCDGRVIPDFIAENANLPLILPYLYPGIKDIVKGVNFASGGSGALDT 124

Query: 124 TFPESVVTFRAQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSNDYGAFDPQSKVY 183
           T PESV+ FR Q+G+FK+ME  LR  LG SETKKLLSRAVYLIA GSNDYGAFDP S VY
Sbjct: 125 TSPESVIPFRTQLGYFKQMETKLRNKLGASETKKLLSRAVYLIATGSNDYGAFDPNSTVY 184

Query: 184 QSYTTEQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEALIYHGKVDWVEQ 243
           QSYTT+Q+VDSVI NM+  +E IYK+GGRKFS+LNV PLDRLP +QEALIYHG+VDW EQ
Sbjct: 185 QSYTTKQFVDSVIANMSSVLEGIYKIGGRKFSLLNVAPLDRLPAIQEALIYHGRVDWAEQ 244

Query: 244 FKQFALLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKYGMKEVKSGCCGIG 303
           FK F++LHN++LPKALQKL +KLKGF+YSH DFYTALN+IID+P+KYGMKEVKSGCCGIG
Sbjct: 245 FKNFSVLHNQQLPKALQKLQQKLKGFIYSHADFYTALNDIIDNPTKYGMKEVKSGCCGIG 304

Query: 304 PLRGNNTCGGKRGIKEYELCGNPEEYVFFDATHLTDRAYHLIADMMWNGTSKITSPLNVK 363
           PL+G N+CGGKRGIKEYELCGNPEEYVFFDATH T   Y +IA +MWNGTS IT+PLNVK
Sbjct: 305 PLKGTNSCGGKRGIKEYELCGNPEEYVFFDATHGTHTLYQIIAQIMWNGTSNITTPLNVK 364

Query: 364 SLFYI 367
           SLFYI
Sbjct: 365 SLFYI 365

BLAST of Lag0032769 vs. NCBI nr
Match: XP_004143991.1 (GDSL esterase/lipase 1 [Cucumis sativus])

HSP 1 Score: 533.5 bits (1373), Expect = 1.4e-147
Identity = 259/366 (70.77%), Postives = 297/366 (81.15%), Query Frame = 0

Query: 5   KLLSCVFLAILAISSLSPHSCEPWPKQELRPLFVFGDSLYDVGNNNYINTIAI--ANFPP 64
           KL+ C  L + ++  +    CE   K    PLFVFGDS+YDVGNNNYINT  I  ANFPP
Sbjct: 7   KLVLCGLLLLCSLGII--QICEALGKD--HPLFVFGDSIYDVGNNNYINTTTISQANFPP 66

Query: 65  YGETFFKFPSGRFSDGREIPDFIAEYANLPMILPYLYPGIKDFVKGVNFASGGAGALDET 124
           YG+TFF+FP+GRFSDGR IPDFIAEYA LP+ILPYLYPGIKDFVKGVNFASGGAG LD T
Sbjct: 67  YGQTFFRFPTGRFSDGRVIPDFIAEYAKLPLILPYLYPGIKDFVKGVNFASGGAGVLDTT 126

Query: 125 FPESVVTFRAQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSNDYGAFDPQS-KVY 184
           FP  VVT R QV +FKEMER+LRK LG S+TKKLLS+AVYLIAIGS DY AFDP+S  +Y
Sbjct: 127 FPGYVVTLRRQVNYFKEMERSLRKKLGTSKTKKLLSKAVYLIAIGSGDYDAFDPKSNSLY 186

Query: 185 QSYTTEQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEALIYHGKVD-WVE 244
           QSYTT+QYVD VIGNMT FIEEIYK GGRKFSVLN+GP+D LP +QEA+I H +   W+E
Sbjct: 187 QSYTTQQYVDLVIGNMTSFIEEIYKTGGRKFSVLNIGPIDHLPAVQEAIISHYRTPAWME 246

Query: 245 QFKQFALLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKYGMKEVKSGCCGI 304
           QFKQF  LHNEKLPKALQ LA+K KG +YSH DF+TA++ II HP+KYGMKEVKSGCCG 
Sbjct: 247 QFKQFIGLHNEKLPKALQNLAQKFKGLLYSHTDFHTAISNIIHHPTKYGMKEVKSGCCGS 306

Query: 305 GPLRGNNTCGGKRGIKEYELCGNPEEYVFFDATHLTDRAYHLIADMMWNGTSKITSPLNV 364
           G  RG ++CGG RGIKEYELC NPEE+VFFDA H TDR Y  +A+MMW GTS IT+P+N+
Sbjct: 307 GAFRGKSSCGGMRGIKEYELCENPEEHVFFDANHGTDRIYKFVAEMMWTGTSNITTPINL 366

Query: 365 KSLFYI 367
            SLFY+
Sbjct: 367 NSLFYM 368

BLAST of Lag0032769 vs. NCBI nr
Match: XP_008460337.1 (PREDICTED: GDSL esterase/lipase 1-like [Cucumis melo])

HSP 1 Score: 411.4 bits (1056), Expect = 8.3e-111
Identity = 195/358 (54.47%), Postives = 261/358 (72.91%), Query Frame = 0

Query: 10  VFLAILAISSLSPHSCEPWPKQELR-PLFVFGDSLYDVGNNNYINTIAIA--NFPPYGET 69
           VFL I+ +   +   C    KQ+   PLFVFGDS+ D+GNNNYINTI IA  N+PPYG T
Sbjct: 11  VFLFIVVVHFFTVGDCISVCKQDHNVPLFVFGDSIVDIGNNNYINTIPIARSNYPPYGLT 70

Query: 70  FFKFPSGRFSDGREIPDFIAEYANLPMILPYLYPGIKDFVKGVNFASGGAGALDETFPES 129
           FFK PSGR+SDGR +PDF A+YANL ++LPYLYPG K ++ G+NFASGGAGALDE     
Sbjct: 71  FFKCPSGRWSDGRVVPDFFAQYANLQLLLPYLYPGNKHYINGINFASGGAGALDEINRGL 130

Query: 130 VVTFRAQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSNDYGAFDPQSKVYQSYTT 189
           V++ + Q   FK++E+ LRK +G ++ K LLSRAVYLI+IG+NDY  F   SK++ SY+ 
Sbjct: 131 VISLKTQARSFKKVEKILRKQIGKTQAKALLSRAVYLISIGTNDYRTFASNSKLFDSYSF 190

Query: 190 EQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEALIYHGKVDWVEQFKQFA 249
           E+YVD VIGN+T  I+EIYK GGRKF V+N+   + +P + EA+   GK   +EQ  Q  
Sbjct: 191 EEYVDLVIGNLTSVIKEIYKNGGRKFVVMNLWSFNHVPAVLEAVASQGKDAQLEQLNQLV 250

Query: 250 LLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKYGMKEVKSGCCGIGPLRGN 309
            +HN++L KA+QKL  +L+GF YS+VD Y    EI ++P+K+G+KEVK+ CCG G  RG 
Sbjct: 251 EMHNKQLYKAVQKLTTELQGFRYSYVDSYKVFEEITNNPAKHGLKEVKNACCGSGVYRGI 310

Query: 310 NTCGGKRGIKEYELCGNPEEYVFFDATHLTDRAYHLIADMMWNGTSKITSPLNVKSLF 365
            +CGGK  +KEYELCGNP+E++FFD+ H +D+AY ++A+M WNG    ++P+NVKSLF
Sbjct: 311 QSCGGKGNVKEYELCGNPKEHLFFDSNHGSDKAYQILAEMAWNGDLNASTPVNVKSLF 368

BLAST of Lag0032769 vs. NCBI nr
Match: XP_022152561.1 (GDSL esterase/lipase 1-like, partial [Momordica charantia])

HSP 1 Score: 409.5 bits (1051), Expect = 3.1e-110
Identity = 197/335 (58.81%), Postives = 251/335 (74.93%), Query Frame = 0

Query: 35  PLFVFGDSLYDVGNNNYINTIAIA--NFPPYGETFFKFPSGRFSDGREIPDFIAEYANLP 94
           PLFV GDS  D GNNNYINTI +A  N+ PYG+TFFKFP+GR+SDGR +PDF+AEYANLP
Sbjct: 19  PLFVLGDSFVDTGNNNYINTIPVAQSNYSPYGQTFFKFPTGRWSDGRVLPDFLAEYANLP 78

Query: 95  MILPYLYPGIKDF-VKGVNFASGGAGALDETFPESVVTFRAQVGHFKEMERTLRKSLGIS 154
           ++LPYL+PG   F   G+NFASGGAGAL +T    V++ + Q+ +F +++R LRK LG S
Sbjct: 79  LLLPYLHPGNNHFYTHGINFASGGAGALHQTHQGLVISLKTQLSYFNKVKRILRKQLGGS 138

Query: 155 -ETKKLLSRAVYLIAIGSNDYGAFDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGR 214
            + + LLSR VYLI+IG+NDYGAF   SK+  SY+ EQYVD VIGN+T  I EI+K GGR
Sbjct: 139 TQAETLLSRGVYLISIGANDYGAFASDSKLLHSYSLEQYVDMVIGNLTSAITEIHKRGGR 198

Query: 215 KFSVLNVGPLDRLPQMQEALIYH-GKVDWVEQFKQFALLHNEKLPKALQKLAEKLKGFVY 274
           KF VLNV PL+ +P ++EA+    GK   VEQ  Q   LHN +L KALQKL+ +L GF+Y
Sbjct: 199 KFVVLNVWPLNHVPAVEEAVASQAGKDSRVEQLTQLVELHNRQLYKALQKLSTELDGFIY 258

Query: 275 SHVDFYTALNEIIDHPSKYGMKEVKSGCCGIGPLRGNNTCGGKRGIKEYELCGNPEEYVF 334
           S+ DFY  +N II +P+KYG+KEVK+ CCG G  RG  +CGGK   KEYELCGNP EY+F
Sbjct: 259 SYADFYNVINGIISNPAKYGIKEVKAACCGSGKFRGIQSCGGKGDAKEYELCGNPNEYLF 318

Query: 335 FDATHLTDRAYHLIADMMWNGTSKITSPLNVKSLF 365
           FD+ H +DR Y ++A+M+WNG S+ + P+NVKSLF
Sbjct: 319 FDSNHGSDRGYKILAEMIWNGDSESSRPVNVKSLF 353

BLAST of Lag0032769 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.1e-93
Identity = 182/373 (48.79%), Postives = 243/373 (65.15%), Query Frame = 0

Query: 1   MAKSKLLSCVFLAILAISSLSPHSCEPWPK--QELRPLFVFGDSLYDVGNNNYINTIAI- 60
           M  S+L+S  FLA   I S+   +C            LFVFGDS++D GNNNYI+T++  
Sbjct: 1   MENSQLVSITFLAYTIIISIGSINCIDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSV 60

Query: 61  -ANFPPYGETFFKFPSGRFSDGREIPDFIAEYANLPMILPYLYP--GIKDFVKGVNFASG 120
            +N+ PYG+T FK P+GR SDGR IPDFIAEYA LP+I P L P  G   F  GVNFASG
Sbjct: 61  RSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASG 120

Query: 121 GAGALDETFPESVVTFRAQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSNDYG-A 180
           GAGAL  TF   V+  R Q+ +FK++E  LR  LG +E K+++SRAVYL  IG NDY   
Sbjct: 121 GAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYP 180

Query: 181 FDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEALIYH 240
           F   S ++QS + E+YVD V+GNMT   +E+Y +GGRKF +LN GP D  P     +I  
Sbjct: 181 FTTNSSLFQSISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAP--ASLVIDQ 240

Query: 241 GKV-DWVEQFKQFALLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKYGMKE 300
            K+    +   +   +HNEKL   L++L  +L GF Y+  D++T+L+E ++ PSKYG KE
Sbjct: 241 TKIRSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKE 300

Query: 301 VKSGCCGIGPLRGNNTCGGKRGIKE-YELCGNPEEYVFFDATHLTDRAYHLIADMMWNGT 360
            K  CCG GPLRG NTCGG+ G+ + YELC N  +Y+FFD  HLT++A   IA+++W+G 
Sbjct: 301 GKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGP 360

Query: 361 SKITSPLNVKSLF 365
           + IT P N+K+LF
Sbjct: 361 TNITGPYNLKALF 371

BLAST of Lag0032769 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.1e-89
Identity = 174/375 (46.40%), Postives = 240/375 (64.00%), Query Frame = 0

Query: 1   MAKSKLLSCVFLAILAISSLSPHSCEPWPKQEL----RPLFVFGDSLYDVGNNNYINTIA 60
           M  S+    +F A   I  +   +C       L      LFVFGDS++D GNNNYI+T+ 
Sbjct: 1   MENSRSTLIIFFAYTTIILIGSINCRDNNNNNLVTNQSALFVFGDSVFDAGNNNYIDTLP 60

Query: 61  I--ANFPPYGETFFKFPSGRFSDGREIPDFIAEYANLPMILPYLYP--GIKDFVKGVNFA 120
              +N+ PYG+T FKFP+GR SDGR IPDFIAEYA LP+I  YL P  G   F  GV+FA
Sbjct: 61  SFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGVSFA 120

Query: 121 SGGAGALDETFPESVVTFRAQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSNDYG 180
           S GAGAL  TFP  V+  ++Q+ +FK++E+ LR +LG ++ K ++SRAVYL  IG NDY 
Sbjct: 121 SAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQ 180

Query: 181 -AFDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEALI 240
             F   S ++QS   E YVD V+GN T  I+E+YK+GGRKF  LN+G  D  P     +I
Sbjct: 181 YPFSTNSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAP--ASLII 240

Query: 241 YHGKV-DWVEQFKQFALLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKYGM 300
              K+    +   +   LHNEKL   L++L  +L GF Y+  D++T+L+  +++PSKYG 
Sbjct: 241 DQTKIGTCFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYGF 300

Query: 301 KEVKSGCCGIGPLRGNNTCGGKRGIKE-YELCGNPEEYVFFDATHLTDRAYHLIADMMWN 360
           KE K  CCG GPLRG NTCGG+ G+ + YELC    +Y+FFD  HLT++A+  IA+++W+
Sbjct: 301 KEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWS 360

Query: 361 GTSKITSPLNVKSLF 365
           G + +T P N+++LF
Sbjct: 361 GPTNVTKPYNLQALF 373

BLAST of Lag0032769 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 324.3 bits (830), Expect = 1.7e-87
Identity = 169/340 (49.71%), Postives = 222/340 (65.29%), Query Frame = 0

Query: 36  LFVFGDSLYDVGNNNYINTIAI--ANFPPYGETFFKFPSGRFSDGREIPDFIAEYANLPM 95
           LF+FGDS  D GNNNYINT  +  ANFPPYG+TFF  P+GRFSDGR I DFIAEYANLP+
Sbjct: 49  LFLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPL 108

Query: 96  ILPYLYPG-IKDFVKGVNFASGGAGALDETFPESVVTFRAQVGHFKEMERTLRKSLGISE 155
           I P+L PG  +  + GVNFAS GAGAL ETF  SV+  R Q+ H+K++ER  R + G  E
Sbjct: 109 IPPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEE 168

Query: 156 TKKLLSRAVYLIAIGSNDYGAFDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGRKF 215
           +KK +SRAVYLI+IGSNDY +    ++     +  Q+VD VIGN+T FI EIYK+GGRKF
Sbjct: 169 SKKRISRAVYLISIGSNDYSSIFLTNQSL-PISMSQHVDIVIGNLTTFIHEIYKIGGRKF 228

Query: 216 SVLNVGPLDRLPQMQEALIYHGKVD--WVEQFKQFALLHNEKLPKALQKLAEKLKGFVYS 275
             LNV  L   P ++   I   K D   +    + A +HN  L   L ++  ++KGF +S
Sbjct: 229 GFLNVPDLGCFPALR---ILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFS 288

Query: 276 HVDFYTALNEIIDHPSKYGMKEVKSGCCGIGPLRGNNTCGGKRGIKEYELCGNPEEYVFF 335
             D   +L   + HPSK+G KE +  CCG G  RG  +CGGKR +KEY+LC NP++Y+F+
Sbjct: 289 LFDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFW 348

Query: 336 DATHLTDRAYHLIADMMWNG----TSKITSPLNVKSLFYI 367
           D+ HLT   Y+  A+++WNG     S +  P N+ +LF I
Sbjct: 349 DSLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQI 384

BLAST of Lag0032769 vs. ExPASy Swiss-Prot
Match: Q9LJP1 (GDSL esterase/lipase 4 OS=Arabidopsis thaliana OX=3702 GN=GLIP4 PE=2 SV=2)

HSP 1 Score: 314.3 bits (804), Expect = 1.8e-84
Identity = 166/377 (44.03%), Postives = 239/377 (63.40%), Query Frame = 0

Query: 1   MAKSKLLSCVFLAILAISSLS--PHSCEPWPKQELRPLFVFGDSLYDVGNNNYINTIAI- 60
           MA  +  S + +  +   SLS    SC+   K     LF FGDSL++ GNNNY ++I+  
Sbjct: 1   MASPRFNSIIIILFICTISLSIVSISCKEDLKTNQAALFAFGDSLFEAGNNNYFDSISSF 60

Query: 61  -ANFPPYGETFFKFPSGRFSDGREIPDFIAEYANLPMILPYLYPGIKD--FVKGVNFASG 120
            +NF PYG+T FKFP+GR SDGR + DFIAEYA LP+I P L PG  +     G+NFA+ 
Sbjct: 61  RSNFWPYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATT 120

Query: 121 GAGALDETFPESVVTFR----AQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSND 180
            AG    TFP SV         Q+ +FK +E+TLR +LG +E ++++S+AVYL  IG+ND
Sbjct: 121 AAGVFAGTFPGSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGAND 180

Query: 181 YG-AFDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEA 240
           Y   F   +  + + T E+++D VIGN T  IEE+YK+G RKF  L++GP    P     
Sbjct: 181 YQYPFFANTSTFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTP--SAL 240

Query: 241 LIYHGKV-DWVEQFKQFALLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKY 300
           +I   K+    E   +   LHN++ PK L++L  +L GF Y+  DF+T+L++ I++PS+Y
Sbjct: 241 IINSTKIGSCFEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRY 300

Query: 301 GMKEVKSGCCGIGPLRGNNTCGGKRGIKE-YELCGNPEEYVFFDATHLTDRAYHLIADMM 360
           G KE +  CCG GPLRG NTCG + G  + Y+LC N ++YVFFD +HLT+ A+  IA+++
Sbjct: 301 GFKEGEMACCGSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELI 360

Query: 361 WNGTSKITSPLNVKSLF 365
           W+G   +T+P N+K+LF
Sbjct: 361 WSGPPNVTAPYNLKTLF 375

BLAST of Lag0032769 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 312.4 bits (799), Expect = 6.9e-84
Identity = 166/338 (49.11%), Postives = 221/338 (65.38%), Query Frame = 0

Query: 36  LFVFGDSLYDVGNNNYINTIAI--ANFPPYGETFFKFPSGRFSDGREIPDFIAEYANLPM 95
           LFVFGDSL+D GNNNYINT++   +N  PYG+T FKFP+GR SDG        E A LP 
Sbjct: 36  LFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKAWLPS 95

Query: 96  ILPYLYP--GIKDFVKGVNFASGGAGALDETFPESVVTFRAQVGHFKEMERTLRKSLGIS 155
           I P L P  G   F  GV+FAS GAGAL E+F   V+    Q+ +FK++E++LR  LG +
Sbjct: 96  IPPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSELGDA 155

Query: 156 ETKKLLSRAVYLIAIGSNDY-GAFDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGR 215
           ETK++ SRAVYL  IG+NDY   F   S  ++S + E++VD VIGN+T  IEE+YKMGGR
Sbjct: 156 ETKRVFSRAVYLFHIGANDYFYPFSANSSTFKSNSKEKFVDFVIGNITFVIEEVYKMGGR 215

Query: 216 KFSVLNVGPLDRLPQMQEALIYHGKVDWVEQFKQFALL---HNEKLPKALQKLAEKLKGF 275
           KF  LNVGP +  P      +   +      FK  A L   HN+K P  L++L  +L GF
Sbjct: 216 KFGFLNVGPYECSPNS----LIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSGF 275

Query: 276 VYSHVDFYTALNEIIDHPSKYGMKEVKSGCCGIGPLRGNNTCGGKRGIKE-YELCGNPEE 335
            Y+  D++T+L+E I+ PSKYG KE K  CCG GPLRG NTCG + G  + Y LC N  +
Sbjct: 276 RYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVTD 335

Query: 336 YVFFDATHLTDRAYHLIADMMWNGTSKITSPLNVKSLF 365
           Y+F+D++HLT++A+  IA+++WNG   +T P N+K+LF
Sbjct: 336 YLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALF 362

BLAST of Lag0032769 vs. ExPASy TrEMBL
Match: A0A6J1E4I7 (GDSL esterase/lipase 1-like OS=Momordica charantia OX=3673 GN=LOC111025987 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 4.8e-173
Identity = 294/363 (80.99%), Postives = 322/363 (88.71%), Query Frame = 0

Query: 4   SKLLSCVFLAILAISSLSPHSCEPWPKQELRPLFVFGDSLYDVGNNNYINTIAIANFPPY 63
           SKL+SCV L ILA+S LSPHSCEP P  E  PLFVFGDSLYDVG NNYINT A ANFPPY
Sbjct: 5   SKLVSCVVLGILAMSCLSPHSCEPEP--EPVPLFVFGDSLYDVGINNYINTSAQANFPPY 64

Query: 64  GETFFKFPSGRFSDGREIPDFIAEYANLPMILPYLYPGIKDFVKGVNFASGGAGALDETF 123
           GETFFKFP+GRFSDGR IPDFIAEYANLP+ILPYLYPGIKD+VKGVNFASGGAGALD+TF
Sbjct: 65  GETFFKFPTGRFSDGRVIPDFIAEYANLPLILPYLYPGIKDYVKGVNFASGGAGALDQTF 124

Query: 124 PESVVTFRAQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSNDYGAFDPQSKVYQS 183
           P+  VT R Q+G+FKEME  LR  LG SETKKLLSRAVYLIAIG NDYGAFD    VYQS
Sbjct: 125 PQLAVTLRTQLGYFKEMETKLRNKLGTSETKKLLSRAVYLIAIGGNDYGAFDSNFTVYQS 184

Query: 184 YTTEQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEALIYHGKVDWVEQFK 243
           YTTEQYVDSVIGNM+  I+ IYKMGGRKFSVLNVGP+DRLP M+EA IYHG+VDWVEQ +
Sbjct: 185 YTTEQYVDSVIGNMSSVIKGIYKMGGRKFSVLNVGPMDRLPAMREAAIYHGRVDWVEQVE 244

Query: 244 QFALLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKYGMKEVKSGCCGIGPL 303
            FA+LHN++LPKALQKL +KLKGF+YSH DFYTALN+IID+P+KYG+KEVKSGCCGIGPL
Sbjct: 245 NFAVLHNQQLPKALQKLQQKLKGFIYSHTDFYTALNDIIDNPTKYGIKEVKSGCCGIGPL 304

Query: 304 RGNNTCGGKRGIKEYELCGNPEEYVFFDATHLTDRAYHLIADMMWNGTSKITSPLNVKSL 363
           RG N+CGGKRGIKEYELCGNPEEYVFFDATHLT RAY +IA +MW GTS IT+PLNVKSL
Sbjct: 305 RGTNSCGGKRGIKEYELCGNPEEYVFFDATHLTHRAYQIIAQIMWTGTSNITTPLNVKSL 364

Query: 364 FYI 367
           FYI
Sbjct: 365 FYI 365

BLAST of Lag0032769 vs. ExPASy TrEMBL
Match: A0A6J1E0K8 (GDSL esterase/lipase 1-like OS=Momordica charantia OX=3673 GN=LOC111026056 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.4e-166
Identity = 284/365 (77.81%), Postives = 315/365 (86.30%), Query Frame = 0

Query: 4   SKLLSCVFLAILAISSLSPHSCEPWPKQELRPLFVFGDSLYDVGNNNYINTI--AIANFP 63
           SKL+SCV L ILA+  LSPHSC   P     PLFVFGDSLYDVGNNNYINT   A ANFP
Sbjct: 5   SKLVSCVILGILAMRCLSPHSCHLEPV----PLFVFGDSLYDVGNNNYINTTAGAQANFP 64

Query: 64  PYGETFFKFPSGRFSDGREIPDFIAEYANLPMILPYLYPGIKDFVKGVNFASGGAGALDE 123
           PYGETFFKFP+GRF DGR IPDFIAE ANLP+ILPYLYPGIKD VKGVNFASGG+GALD 
Sbjct: 65  PYGETFFKFPTGRFCDGRVIPDFIAENANLPLILPYLYPGIKDIVKGVNFASGGSGALDT 124

Query: 124 TFPESVVTFRAQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSNDYGAFDPQSKVY 183
           T PESV+ FR Q+G+FK+ME  LR  LG SETKKLLSRAVYLIA GSNDYGAFDP S VY
Sbjct: 125 TSPESVIPFRTQLGYFKQMETKLRNKLGASETKKLLSRAVYLIATGSNDYGAFDPNSTVY 184

Query: 184 QSYTTEQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEALIYHGKVDWVEQ 243
           QSYTT+Q+VDSVI NM+  +E IYK+GGRKFS+LNV PLDRLP +QEALIYHG+VDW EQ
Sbjct: 185 QSYTTKQFVDSVIANMSSVLEGIYKIGGRKFSLLNVAPLDRLPAIQEALIYHGRVDWAEQ 244

Query: 244 FKQFALLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKYGMKEVKSGCCGIG 303
           FK F++LHN++LPKALQKL +KLKGF+YSH DFYTALN+IID+P+KYGMKEVKSGCCGIG
Sbjct: 245 FKNFSVLHNQQLPKALQKLQQKLKGFIYSHADFYTALNDIIDNPTKYGMKEVKSGCCGIG 304

Query: 304 PLRGNNTCGGKRGIKEYELCGNPEEYVFFDATHLTDRAYHLIADMMWNGTSKITSPLNVK 363
           PL+G N+CGGKRGIKEYELCGNPEEYVFFDATH T   Y +IA +MWNGTS IT+PLNVK
Sbjct: 305 PLKGTNSCGGKRGIKEYELCGNPEEYVFFDATHGTHTLYQIIAQIMWNGTSNITTPLNVK 364

Query: 364 SLFYI 367
           SLFYI
Sbjct: 365 SLFYI 365

BLAST of Lag0032769 vs. ExPASy TrEMBL
Match: A0A0A0KP02 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G155530 PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 2.3e-119
Identity = 209/284 (73.59%), Postives = 238/284 (83.80%), Query Frame = 0

Query: 85  IAEYANLPMILPYLYPGIKDFVKGVNFASGGAGALDETFPESVVTFRAQVGHFKEMERTL 144
           I+EYA LP+ILPYLYPGIKDFVKGVNFASGGAG LD TFP  VVT R QV +FKEMER+L
Sbjct: 21  ISEYAKLPLILPYLYPGIKDFVKGVNFASGGAGVLDTTFPGYVVTLRRQVNYFKEMERSL 80

Query: 145 RKSLGISETKKLLSRAVYLIAIGSNDYGAFDPQS-KVYQSYTTEQYVDSVIGNMTGFIEE 204
           RK LG S+TKKLLS+AVYLIAIGS DY AFDP+S  +YQSYTT+QYVD VIGNMT FIEE
Sbjct: 81  RKKLGTSKTKKLLSKAVYLIAIGSGDYDAFDPKSNSLYQSYTTQQYVDLVIGNMTSFIEE 140

Query: 205 IYKMGGRKFSVLNVGPLDRLPQMQEALIYHGKVD-WVEQFKQFALLHNEKLPKALQKLAE 264
           IYK GGRKFSVLN+GP+D LP +QEA+I H +   W+EQFKQF  LHNEKLPKALQ LA+
Sbjct: 141 IYKTGGRKFSVLNIGPIDHLPAVQEAIISHYRTPAWMEQFKQFIGLHNEKLPKALQNLAQ 200

Query: 265 KLKGFVYSHVDFYTALNEIIDHPSKYGMKEVKSGCCGIGPLRGNNTCGGKRGIKEYELCG 324
           K KG +YSH DF+TA++ II HP+KYGMKEVKSGCCG G  RG ++CGG RGIKEYELC 
Sbjct: 201 KFKGLLYSHTDFHTAISNIIHHPTKYGMKEVKSGCCGSGAFRGKSSCGGMRGIKEYELCE 260

Query: 325 NPEEYVFFDATHLTDRAYHLIADMMWNGTSKITSPLNVKSLFYI 367
           NPEE+VFFDA H TDR Y  +A+MMW GTS IT+P+N+ SLFYI
Sbjct: 261 NPEEHVFFDANHGTDRIYKFVAEMMWTGTSNITTPINLNSLFYI 304

BLAST of Lag0032769 vs. ExPASy TrEMBL
Match: A0A1S3CBT3 (GDSL esterase/lipase 1-like OS=Cucumis melo OX=3656 GN=LOC103499191 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 4.0e-111
Identity = 195/358 (54.47%), Postives = 261/358 (72.91%), Query Frame = 0

Query: 10  VFLAILAISSLSPHSCEPWPKQELR-PLFVFGDSLYDVGNNNYINTIAIA--NFPPYGET 69
           VFL I+ +   +   C    KQ+   PLFVFGDS+ D+GNNNYINTI IA  N+PPYG T
Sbjct: 11  VFLFIVVVHFFTVGDCISVCKQDHNVPLFVFGDSIVDIGNNNYINTIPIARSNYPPYGLT 70

Query: 70  FFKFPSGRFSDGREIPDFIAEYANLPMILPYLYPGIKDFVKGVNFASGGAGALDETFPES 129
           FFK PSGR+SDGR +PDF A+YANL ++LPYLYPG K ++ G+NFASGGAGALDE     
Sbjct: 71  FFKCPSGRWSDGRVVPDFFAQYANLQLLLPYLYPGNKHYINGINFASGGAGALDEINRGL 130

Query: 130 VVTFRAQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSNDYGAFDPQSKVYQSYTT 189
           V++ + Q   FK++E+ LRK +G ++ K LLSRAVYLI+IG+NDY  F   SK++ SY+ 
Sbjct: 131 VISLKTQARSFKKVEKILRKQIGKTQAKALLSRAVYLISIGTNDYRTFASNSKLFDSYSF 190

Query: 190 EQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEALIYHGKVDWVEQFKQFA 249
           E+YVD VIGN+T  I+EIYK GGRKF V+N+   + +P + EA+   GK   +EQ  Q  
Sbjct: 191 EEYVDLVIGNLTSVIKEIYKNGGRKFVVMNLWSFNHVPAVLEAVASQGKDAQLEQLNQLV 250

Query: 250 LLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKYGMKEVKSGCCGIGPLRGN 309
            +HN++L KA+QKL  +L+GF YS+VD Y    EI ++P+K+G+KEVK+ CCG G  RG 
Sbjct: 251 EMHNKQLYKAVQKLTTELQGFRYSYVDSYKVFEEITNNPAKHGLKEVKNACCGSGVYRGI 310

Query: 310 NTCGGKRGIKEYELCGNPEEYVFFDATHLTDRAYHLIADMMWNGTSKITSPLNVKSLF 365
            +CGGK  +KEYELCGNP+E++FFD+ H +D+AY ++A+M WNG    ++P+NVKSLF
Sbjct: 311 QSCGGKGNVKEYELCGNPKEHLFFDSNHGSDKAYQILAEMAWNGDLNASTPVNVKSLF 368

BLAST of Lag0032769 vs. ExPASy TrEMBL
Match: A0A6J1DI34 (GDSL esterase/lipase 1-like OS=Momordica charantia OX=3673 GN=LOC111020259 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.5e-110
Identity = 197/335 (58.81%), Postives = 251/335 (74.93%), Query Frame = 0

Query: 35  PLFVFGDSLYDVGNNNYINTIAIA--NFPPYGETFFKFPSGRFSDGREIPDFIAEYANLP 94
           PLFV GDS  D GNNNYINTI +A  N+ PYG+TFFKFP+GR+SDGR +PDF+AEYANLP
Sbjct: 19  PLFVLGDSFVDTGNNNYINTIPVAQSNYSPYGQTFFKFPTGRWSDGRVLPDFLAEYANLP 78

Query: 95  MILPYLYPGIKDF-VKGVNFASGGAGALDETFPESVVTFRAQVGHFKEMERTLRKSLGIS 154
           ++LPYL+PG   F   G+NFASGGAGAL +T    V++ + Q+ +F +++R LRK LG S
Sbjct: 79  LLLPYLHPGNNHFYTHGINFASGGAGALHQTHQGLVISLKTQLSYFNKVKRILRKQLGGS 138

Query: 155 -ETKKLLSRAVYLIAIGSNDYGAFDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGR 214
            + + LLSR VYLI+IG+NDYGAF   SK+  SY+ EQYVD VIGN+T  I EI+K GGR
Sbjct: 139 TQAETLLSRGVYLISIGANDYGAFASDSKLLHSYSLEQYVDMVIGNLTSAITEIHKRGGR 198

Query: 215 KFSVLNVGPLDRLPQMQEALIYH-GKVDWVEQFKQFALLHNEKLPKALQKLAEKLKGFVY 274
           KF VLNV PL+ +P ++EA+    GK   VEQ  Q   LHN +L KALQKL+ +L GF+Y
Sbjct: 199 KFVVLNVWPLNHVPAVEEAVASQAGKDSRVEQLTQLVELHNRQLYKALQKLSTELDGFIY 258

Query: 275 SHVDFYTALNEIIDHPSKYGMKEVKSGCCGIGPLRGNNTCGGKRGIKEYELCGNPEEYVF 334
           S+ DFY  +N II +P+KYG+KEVK+ CCG G  RG  +CGGK   KEYELCGNP EY+F
Sbjct: 259 SYADFYNVINGIISNPAKYGIKEVKAACCGSGKFRGIQSCGGKGDAKEYELCGNPNEYLF 318

Query: 335 FDATHLTDRAYHLIADMMWNGTSKITSPLNVKSLF 365
           FD+ H +DR Y ++A+M+WNG S+ + P+NVKSLF
Sbjct: 319 FDSNHGSDRGYKILAEMIWNGDSESSRPVNVKSLF 353

BLAST of Lag0032769 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 344.0 bits (881), Expect = 1.5e-94
Identity = 182/373 (48.79%), Postives = 243/373 (65.15%), Query Frame = 0

Query: 1   MAKSKLLSCVFLAILAISSLSPHSCEPWPK--QELRPLFVFGDSLYDVGNNNYINTIAI- 60
           M  S+L+S  FLA   I S+   +C            LFVFGDS++D GNNNYI+T++  
Sbjct: 1   MENSQLVSITFLAYTIIISIGSINCIDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSV 60

Query: 61  -ANFPPYGETFFKFPSGRFSDGREIPDFIAEYANLPMILPYLYP--GIKDFVKGVNFASG 120
            +N+ PYG+T FK P+GR SDGR IPDFIAEYA LP+I P L P  G   F  GVNFASG
Sbjct: 61  RSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASG 120

Query: 121 GAGALDETFPESVVTFRAQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSNDYG-A 180
           GAGAL  TF   V+  R Q+ +FK++E  LR  LG +E K+++SRAVYL  IG NDY   
Sbjct: 121 GAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYP 180

Query: 181 FDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEALIYH 240
           F   S ++QS + E+YVD V+GNMT   +E+Y +GGRKF +LN GP D  P     +I  
Sbjct: 181 FTTNSSLFQSISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAP--ASLVIDQ 240

Query: 241 GKV-DWVEQFKQFALLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKYGMKE 300
            K+    +   +   +HNEKL   L++L  +L GF Y+  D++T+L+E ++ PSKYG KE
Sbjct: 241 TKIRSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKE 300

Query: 301 VKSGCCGIGPLRGNNTCGGKRGIKE-YELCGNPEEYVFFDATHLTDRAYHLIADMMWNGT 360
            K  CCG GPLRG NTCGG+ G+ + YELC N  +Y+FFD  HLT++A   IA+++W+G 
Sbjct: 301 GKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGP 360

Query: 361 SKITSPLNVKSLF 365
           + IT P N+K+LF
Sbjct: 361 TNITGPYNLKALF 371

BLAST of Lag0032769 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 324.3 bits (830), Expect = 1.2e-88
Identity = 169/340 (49.71%), Postives = 222/340 (65.29%), Query Frame = 0

Query: 36  LFVFGDSLYDVGNNNYINTIAI--ANFPPYGETFFKFPSGRFSDGREIPDFIAEYANLPM 95
           LF+FGDS  D GNNNYINT  +  ANFPPYG+TFF  P+GRFSDGR I DFIAEYANLP+
Sbjct: 49  LFLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPL 108

Query: 96  ILPYLYPG-IKDFVKGVNFASGGAGALDETFPESVVTFRAQVGHFKEMERTLRKSLGISE 155
           I P+L PG  +  + GVNFAS GAGAL ETF  SV+  R Q+ H+K++ER  R + G  E
Sbjct: 109 IPPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEE 168

Query: 156 TKKLLSRAVYLIAIGSNDYGAFDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGRKF 215
           +KK +SRAVYLI+IGSNDY +    ++     +  Q+VD VIGN+T FI EIYK+GGRKF
Sbjct: 169 SKKRISRAVYLISIGSNDYSSIFLTNQSL-PISMSQHVDIVIGNLTTFIHEIYKIGGRKF 228

Query: 216 SVLNVGPLDRLPQMQEALIYHGKVD--WVEQFKQFALLHNEKLPKALQKLAEKLKGFVYS 275
             LNV  L   P ++   I   K D   +    + A +HN  L   L ++  ++KGF +S
Sbjct: 229 GFLNVPDLGCFPALR---ILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFS 288

Query: 276 HVDFYTALNEIIDHPSKYGMKEVKSGCCGIGPLRGNNTCGGKRGIKEYELCGNPEEYVFF 335
             D   +L   + HPSK+G KE +  CCG G  RG  +CGGKR +KEY+LC NP++Y+F+
Sbjct: 289 LFDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFW 348

Query: 336 DATHLTDRAYHLIADMMWNG----TSKITSPLNVKSLFYI 367
           D+ HLT   Y+  A+++WNG     S +  P N+ +LF I
Sbjct: 349 DSLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQI 384

BLAST of Lag0032769 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 318.5 bits (815), Expect = 6.8e-87
Identity = 169/361 (46.81%), Postives = 230/361 (63.71%), Query Frame = 0

Query: 1   MAKSKLLSCVFLAILAISSLSPHSCEPWPKQEL----RPLFVFGDSLYDVGNNNYINTIA 60
           M  S+    +F A   I  +   +C       L      LFVFGDS++D GNNNYI+T+ 
Sbjct: 1   MENSRSTLIIFFAYTTIILIGSINCRDNNNNNLVTNQSALFVFGDSVFDAGNNNYIDTLP 60

Query: 61  I--ANFPPYGETFFKFPSGRFSDGREIPDFIAEYANLPMILPYLYP--GIKDFVKGVNFA 120
              +N+ PYG+T FKFP+GR SDGR IPDFIAEYA LP+I  YL P  G   F  GV+FA
Sbjct: 61  SFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGVSFA 120

Query: 121 SGGAGALDETFPESVVTFRAQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSNDYG 180
           S GAGAL  TFP  V+  ++Q+ +FK++E+ LR +LG ++ K ++SRAVYL  IG NDY 
Sbjct: 121 SAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQ 180

Query: 181 -AFDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEALI 240
             F   S ++QS   E YVD V+GN T  I+E+YK+GGRKF  LN+G  D  P     +I
Sbjct: 181 YPFSTNSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAP--ASLII 240

Query: 241 YHGKV-DWVEQFKQFALLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKYGM 300
              K+    +   +   LHNEKL   L++L  +L GF Y+  D++T+L+  +++PSKYG 
Sbjct: 241 DQTKIGTCFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYGF 300

Query: 301 KEVKSGCCGIGPLRGNNTCGGKRGIKE-YELCGNPEEYVFFDATHLTDRAYHLIADMMWN 351
           KE K  CCG GPLRG NTCGG+ G+ + YELC    +Y+FFD  HLT++A+  IA+++W+
Sbjct: 301 KEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWS 359

BLAST of Lag0032769 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 314.3 bits (804), Expect = 1.3e-85
Identity = 166/377 (44.03%), Postives = 239/377 (63.40%), Query Frame = 0

Query: 1   MAKSKLLSCVFLAILAISSLS--PHSCEPWPKQELRPLFVFGDSLYDVGNNNYINTIAI- 60
           MA  +  S + +  +   SLS    SC+   K     LF FGDSL++ GNNNY ++I+  
Sbjct: 1   MASPRFNSIIIILFICTISLSIVSISCKEDLKTNQAALFAFGDSLFEAGNNNYFDSISSF 60

Query: 61  -ANFPPYGETFFKFPSGRFSDGREIPDFIAEYANLPMILPYLYPGIKD--FVKGVNFASG 120
            +NF PYG+T FKFP+GR SDGR + DFIAEYA LP+I P L PG  +     G+NFA+ 
Sbjct: 61  RSNFWPYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATT 120

Query: 121 GAGALDETFPESVVTFR----AQVGHFKEMERTLRKSLGISETKKLLSRAVYLIAIGSND 180
            AG    TFP SV         Q+ +FK +E+TLR +LG +E ++++S+AVYL  IG+ND
Sbjct: 121 AAGVFAGTFPGSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGAND 180

Query: 181 YG-AFDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGRKFSVLNVGPLDRLPQMQEA 240
           Y   F   +  + + T E+++D VIGN T  IEE+YK+G RKF  L++GP    P     
Sbjct: 181 YQYPFFANTSTFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTP--SAL 240

Query: 241 LIYHGKV-DWVEQFKQFALLHNEKLPKALQKLAEKLKGFVYSHVDFYTALNEIIDHPSKY 300
           +I   K+    E   +   LHN++ PK L++L  +L GF Y+  DF+T+L++ I++PS+Y
Sbjct: 241 IINSTKIGSCFEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRY 300

Query: 301 GMKEVKSGCCGIGPLRGNNTCGGKRGIKE-YELCGNPEEYVFFDATHLTDRAYHLIADMM 360
           G KE +  CCG GPLRG NTCG + G  + Y+LC N ++YVFFD +HLT+ A+  IA+++
Sbjct: 301 GFKEGEMACCGSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELI 360

Query: 361 WNGTSKITSPLNVKSLF 365
           W+G   +T+P N+K+LF
Sbjct: 361 WSGPPNVTAPYNLKTLF 375

BLAST of Lag0032769 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 312.4 bits (799), Expect = 4.9e-85
Identity = 166/338 (49.11%), Postives = 221/338 (65.38%), Query Frame = 0

Query: 36  LFVFGDSLYDVGNNNYINTIAI--ANFPPYGETFFKFPSGRFSDGREIPDFIAEYANLPM 95
           LFVFGDSL+D GNNNYINT++   +N  PYG+T FKFP+GR SDG        E A LP 
Sbjct: 36  LFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKAWLPS 95

Query: 96  ILPYLYP--GIKDFVKGVNFASGGAGALDETFPESVVTFRAQVGHFKEMERTLRKSLGIS 155
           I P L P  G   F  GV+FAS GAGAL E+F   V+    Q+ +FK++E++LR  LG +
Sbjct: 96  IPPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSELGDA 155

Query: 156 ETKKLLSRAVYLIAIGSNDY-GAFDPQSKVYQSYTTEQYVDSVIGNMTGFIEEIYKMGGR 215
           ETK++ SRAVYL  IG+NDY   F   S  ++S + E++VD VIGN+T  IEE+YKMGGR
Sbjct: 156 ETKRVFSRAVYLFHIGANDYFYPFSANSSTFKSNSKEKFVDFVIGNITFVIEEVYKMGGR 215

Query: 216 KFSVLNVGPLDRLPQMQEALIYHGKVDWVEQFKQFALL---HNEKLPKALQKLAEKLKGF 275
           KF  LNVGP +  P      +   +      FK  A L   HN+K P  L++L  +L GF
Sbjct: 216 KFGFLNVGPYECSPNS----LIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSGF 275

Query: 276 VYSHVDFYTALNEIIDHPSKYGMKEVKSGCCGIGPLRGNNTCGGKRGIKE-YELCGNPEE 335
            Y+  D++T+L+E I+ PSKYG KE K  CCG GPLRG NTCG + G  + Y LC N  +
Sbjct: 276 RYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVTD 335

Query: 336 YVFFDATHLTDRAYHLIADMMWNGTSKITSPLNVKSLF 365
           Y+F+D++HLT++A+  IA+++WNG   +T P N+K+LF
Sbjct: 336 YLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALF 362