Lag0032731 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0032731
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Poly(A)-specific ribonuclease
Location	chr11: 36804725 .. 36805456 (+)
RNA-Seq Expression	Lag0032731
Synteny	Lag0032731

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGACGGTGGAGAGAAAGGAGAGGGAGAAGAAGAGAAAAGGAGGGGGCGGTGTTGAGTATCTCCCAGAATTTCCCATCGTCGCCATCGATACAGAATTTCCAGGTATTTTCCTGCGGATCCGATTAATCGAAACTGTTATTTAGGTTTTGATTGATTGGCTCGCTCGAAATTTTTTTATCCTCTCGGAATCGGTACTAGGGTTTCTCCGATACGCTCCAAAAGGTGTTTCGGAGGAAGAATTCTACGAGTGTCTTCGATTCAATGTGAACCGATTGAGCATACTCCAACTCGGTCTCACACTCACCAACCAGAACGGAGACATCGGAATCACTTGGGAGTTCAATTTCAGAGGTTTCGATCGCATTTCTGATATGGAAGGGGTTTCTTCGCTCGATTCGTTCCTGAAGAACAAGGGCTTCGATTTCGAAAAATTGAGAAATGACGGAATCGCGCCAGAGGAATTCACTGCCCTGTTTCTTCCGATTTGTCGCAGTGGTCGAATCGATCGATGGATCACCTTCCATGGATCCTACGACATCGCTTACCTCATGAAGCTTCTCAATATCAGATCCATGGCGACCTCCATGGCGATTTTCGCAGCCAACGCGCAGCAATTGTTGGGAACGATCGTCGATCTCAAGTACATGCCGAGAAGATGCAAAAGAGGGAGAGAAGAGCAGGGGGGAGAAGAAGAGAAAAGGATGGGGGAATCGGGGAGAGAGAACTGA

mRNA sequence

ATGGTGACGGTGGAGAGAAAGGAGAGGGAGAAGAAGAGAAAAGGAGGGGGCGGTGTTGAGTATCTCCCAGAATTTCCCATCGTCGCCATCGATACAGAATTTCCAGGGTTTCTCCGATACGCTCCAAAAGGTGTTTCGGAGGAAGAATTCTACGAGTGTCTTCGATTCAATGTGAACCGATTGAGCATACTCCAACTCGGTCTCACACTCACCAACCAGAACGGAGACATCGGAATCACTTGGGAGTTCAATTTCAGAGGTTTCGATCGCATTTCTGATATGGAAGGGGTTTCTTCGCTCGATTCGTTCCTGAAGAACAAGGGCTTCGATTTCGAAAAATTGAGAAATGACGGAATCGCGCCAGAGGAATTCACTGCCCTGTTTCTTCCGATTTGTCGCAGTGGTCGAATCGATCGATGGATCACCTTCCATGGATCCTACGACATCGCTTACCTCATGAAGCTTCTCAATATCAGATCCATGGCGACCTCCATGGCGATTTTCGCAGCCAACGCGCAGCAATTGTTGGGAACGATCGTCGATCTCAAGTACATGCCGAGAAGATGCAAAAGAGGGAGAGAAGAGCAGGGGGGAGAAGAAGAGAAAAGGATGGGGGAATCGGGGAGAGAGAACTGA

Coding sequence (CDS)

ATGGTGACGGTGGAGAGAAAGGAGAGGGAGAAGAAGAGAAAAGGAGGGGGCGGTGTTGAGTATCTCCCAGAATTTCCCATCGTCGCCATCGATACAGAATTTCCAGGGTTTCTCCGATACGCTCCAAAAGGTGTTTCGGAGGAAGAATTCTACGAGTGTCTTCGATTCAATGTGAACCGATTGAGCATACTCCAACTCGGTCTCACACTCACCAACCAGAACGGAGACATCGGAATCACTTGGGAGTTCAATTTCAGAGGTTTCGATCGCATTTCTGATATGGAAGGGGTTTCTTCGCTCGATTCGTTCCTGAAGAACAAGGGCTTCGATTTCGAAAAATTGAGAAATGACGGAATCGCGCCAGAGGAATTCACTGCCCTGTTTCTTCCGATTTGTCGCAGTGGTCGAATCGATCGATGGATCACCTTCCATGGATCCTACGACATCGCTTACCTCATGAAGCTTCTCAATATCAGATCCATGGCGACCTCCATGGCGATTTTCGCAGCCAACGCGCAGCAATTGTTGGGAACGATCGTCGATCTCAAGTACATGCCGAGAAGATGCAAAAGAGGGAGAGAAGAGCAGGGGGGAGAAGAAGAGAAAAGGATGGGGGAATCGGGGAGAGAGAACTGA

Protein sequence

MVTVERKEREKKRKGGGGVEYLPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGITWEFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLPICRSGRIDRWITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYMPRRCKRGREEQGGEEEKRMGESGREN

Homology

[Close]

BLAST of Lag0032731 vs. NCBI nr
Match: XP_022154798.1 (probable CCR4-associated factor 1 homolog 6 [Momordica charantia])

HSP 1 Score: 274.6 bits (701), Expect = 7.0e-70
Identity = 137/199 (68.84%), Postives = 150/199 (75.38%), Query Frame = 0


Query: 20  EYLPEFPIVAIDTEF-----------------------------PGFLRYAPKGVSEEEF 79
           EYLPEFPIVAIDTEF                             PGFLRY PKGVSEEEF
Sbjct: 35  EYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEF 94

Query: 80  YECLRFNVNRLSILQLGLTLTNQNGDIGITWEFNFRGFDRISDMEGVSSLDSFLKNKGFD 139
           Y+CLRFNVNRL+ILQLGLTL NQNG++ +TWEFNFR FD ISDMEGVSSLDSFLKNKGFD
Sbjct: 95  YDCLRFNVNRLNILQLGLTLANQNGNVAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFD 154

Query: 140 FEKLRNDGIAPEEFTALFLPICRSGRIDRWITFHGSYDIAYLMKLLNIRSMATSMAIFAA 190
           F KL+ DGIA E+FT LFLPICRSGRI+RWITFHG YDIAYL+KLL I+ +  SMA FAA
Sbjct: 155 FYKLKRDGIALEDFTTLFLPICRSGRIERWITFHGFYDIAYLLKLLKIKYIPISMATFAA 214

[Close]

BLAST of Lag0032731 vs. NCBI nr
Match: XP_022146384.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 265.8 bits (678), Expect = 3.2e-67
Identity = 125/170 (73.53%), Postives = 140/170 (82.35%), Query Frame = 0


Query: 20  EYLPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGI 79
           EYLPEFP+V ID EFPGFL   PKG+SEEEFY CLRFNVNRL+ILQLGLTL +Q G+I +
Sbjct: 54  EYLPEFPVVVIDMEFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAM 113

Query: 80  TWEFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLPICRSGRIDR 139
           TWEFNFR FD +SDME VSSLDSFLKNKGFDF KL+  GIA E+FT LFLPICRSG+I R
Sbjct: 114 TWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYKLKKYGIAQEDFTTLFLPICRSGQIKR 173

Query: 140 WITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYMPRRC 190
           WITFHG YDI YL+KLL I+S+  SMA FA  AQ LLGT+ DLK+M R C
Sbjct: 174 WITFHGFYDITYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYC 223

[Close]

BLAST of Lag0032731 vs. NCBI nr
Match: XP_022963784.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 264.2 bits (674), Expect = 9.4e-67
Identity = 127/164 (77.44%), Postives = 139/164 (84.76%), Query Frame = 0


Query: 22  LPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGITW 81
           L EFPIVAIDTEFPGFLR+ PKGVS+EEFYECL+FNVNRLSILQLGLTLTN NG IG+TW
Sbjct: 35  LSEFPIVAIDTEFPGFLRWTPKGVSDEEFYECLQFNVNRLSILQLGLTLTNDNGKIGMTW 94

Query: 82  EFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLPICRSGRIDRWI 141
           EFNFR FD+IS+MEGV SLDSFLKNKGF+F  LR+ GIAPEEF   F PI RSGRI RWI
Sbjct: 95  EFNFRDFDKISNMEGVYSLDSFLKNKGFNFNTLRSYGIAPEEFGTQFSPILRSGRIHRWI 154

Query: 142 TFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYM 186
           TFHG YDIAYLMK  NIR M TSM+IFA  A  +LGT+ DLK+M
Sbjct: 155 TFHGLYDIAYLMKFTNIRCMPTSMSIFAITAAHILGTVTDLKHM 198

[Close]

BLAST of Lag0032731 vs. NCBI nr
Match: XP_022963785.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 262.7 bits (670), Expect = 2.7e-66
Identity = 127/166 (76.51%), Postives = 140/166 (84.34%), Query Frame = 0


Query: 22  LPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGITW 81
           L EFPIVA+DTEFPGFLR+ PKGVS+EEFYECL+FNVNRLSILQLGLTLTN NG IG+TW
Sbjct: 32  LSEFPIVAMDTEFPGFLRWTPKGVSDEEFYECLQFNVNRLSILQLGLTLTNDNGKIGMTW 91

Query: 82  EFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLPICRSGRIDRWI 141
           EFNF+ FD+IS+MEGV SLDSFLKNKGF+F  LR+ GIAPEEF   F PI RSGRI RWI
Sbjct: 92  EFNFKDFDKISNMEGVYSLDSFLKNKGFNFNTLRSYGIAPEEFGRQFNPILRSGRIHRWI 151

Query: 142 TFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYMPR 188
           TFHG YDIAYLMKL NI  M TSM+IFA  A  LLGT+ DLK+M R
Sbjct: 152 TFHGLYDIAYLMKLTNITCMPTSMSIFAITAAHLLGTVTDLKHMAR 197

[Close]

BLAST of Lag0032731 vs. NCBI nr
Match: XP_022928830.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 260.8 bits (665), Expect = 1.0e-65
Identity = 124/167 (74.25%), Postives = 143/167 (85.63%), Query Frame = 0


Query: 21  YLPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGIT 80
           YL EFPIVAIDTEFPGFLR+ PKGVS+EEFY+CL+FNVNRLSILQLGLTLT+ NG IG+T
Sbjct: 34  YLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYQCLQFNVNRLSILQLGLTLTDDNGKIGMT 93

Query: 81  WEFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLPICRSGRIDRW 140
           WEFNF+ FD+IS+MEGV SLDSFLKNKGF+F KLR+ GIAP+EF+  F P+ RSGRI +W
Sbjct: 94  WEFNFQDFDQISNMEGVYSLDSFLKNKGFNFNKLRSCGIAPQEFSRQFKPVIRSGRIQKW 153

Query: 141 ITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYMPR 188
           ITFHG YDIAYLMK  NI SM TSM++FA  A  LLGT+ DLK+M R
Sbjct: 154 ITFHGLYDIAYLMKFNNIISMPTSMSMFAMIAAHLLGTVTDLKHMAR 200

[Close]

BLAST of Lag0032731 vs. ExPASy Swiss-Prot
Match: Q9LXM4 (Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=CAF1-8 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.1e-28
Identity = 68/170 (40.00%), Postives = 101/170 (59.41%), Query Frame = 0


Query: 22  LPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGITW 81
           L  +  +AIDTEFP  LR   +  ++EE Y  + F+V+R  ++QLGLTL + NG IG TW
Sbjct: 8   LRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRIGGTW 67

Query: 82  EFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEE-FTALFLPICRSGRIDRW 141
           E NF  F  + D     S++ FL+  G D  K+R +GI  E  F+ +F  + ++ R   W
Sbjct: 68  EINFSDFG-VDDARNEKSIE-FLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRNITW 127

Query: 142 ITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYMPRRCK 191
           +TFHGSYDIAYL+K     ++  +   F+    ++LG++ DLK M  RC+
Sbjct: 128 VTFHGSYDIAYLLKGFTGEALPVTSERFSKAVARVLGSVYDLKVMAGRCE 175

[Close]

BLAST of Lag0032731 vs. ExPASy Swiss-Prot
Match: O64773 (Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=CAF1-5 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.1e-25
Identity = 69/165 (41.82%), Postives = 95/165 (57.58%), Query Frame = 0


Query: 28  VAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGITWEFNFRG 87
           +AIDTEFPG L+  P   S+E  Y  ++FNV+   ++QLGLTL  +   I  TWE N   
Sbjct: 27  IAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLIQLGLTLFGKG--ITKTWEINLSD 86

Query: 88  FDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLPIC--RSGRIDRWITFHG 147
           F+    ++   S+ +FLKN G D +K+R +GI  EEF   F  I   + G++ RW+TF G
Sbjct: 87  FNESKSLKNDKSI-AFLKNNGLDLDKIREEGIGIEEFFMEFSQILNEKHGKM-RWVTFQG 146

Query: 148 SYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIV-DLKYMPRRC 190
           SYD AYL+K L  + +  +   F    QQLLG  V D+K M   C
Sbjct: 147 SYDKAYLLKGLTRKPLPETSKEFDETVQQLLGRFVYDVKKMAGLC 187

[Close]

BLAST of Lag0032731 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.9e-23
Identity = 65/184 (35.33%), Postives = 97/184 (52.72%), Query Frame = 0


Query: 20  EYLPEFPIVAIDTEFPGFLRYAPKGV---SEEEFYECLRFNVNRLSILQLGLTLTNQNGD 79
           + + +FP VA+DTEFPG +   P G    + +  YE L+ NVN L ++QLGLT +N+ G+
Sbjct: 29  DVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVNILKMIQLGLTFSNEQGN 88

Query: 80  IG-------ITWEFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFL 139
           +          W+FNFR FD  SD+  + S++  LK  G D  K   DGI  + F  L +
Sbjct: 89  LPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKNTLDGIDSKRFAELLM 148

Query: 140 PICRSGRI----DRWITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYM 190
               SG +      W+TFH  YD  YL+KLL  +++  S   F         T+ D+K++
Sbjct: 149 ---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLINVYFPTVYDIKHL 207

[Close]

BLAST of Lag0032731 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.1e-23
Identity = 61/182 (33.52%), Postives = 93/182 (51.10%), Query Frame = 0


Query: 20  EYLPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGI 79
           E L  FP++A DTE+PG +       S +E Y  ++ NV    ++Q G TL N  G+IG 
Sbjct: 26  ECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVENTKLIQCGFTLFNAKGEIGG 85

Query: 80  TWEFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLP----ICRSG 139
            WE NF  F   SD     S++ FL+  G D +K+R++G+  + F   F P    + RS 
Sbjct: 86  VWEINFSNFGDPSDTRNELSIE-FLRRHGLDLQKIRDEGV--DMFGYGFFPKLMTVFRSQ 145

Query: 140 RIDRWITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYMPRRCKRGREE 198
           +   ++TF G+YD AY + +LN   +  +   FA    ++ G + D K M   C+   E 
Sbjct: 146 KHVEFVTFQGAYDFAYFLSILNHGKLPETHGEFATEVVKVFGQVYDTKVMAGFCEGLGEH 204

[Close]

BLAST of Lag0032731 vs. ExPASy Swiss-Prot
Match: Q9S9P2 (Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=CAF1-2 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-22
Identity = 60/185 (32.43%), Postives = 98/185 (52.97%), Query Frame = 0


Query: 22  LPEFPIVAIDTEFPGFL--------RYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQ 81
           + +FP VA+DTEFPG +           P  +  E  Y+ L+ NVN L ++QLGLTL+++
Sbjct: 35  IDDFPYVAMDTEFPGIVCKTVTANPNPNPYSIHYEYNYDTLKANVNMLKLIQLGLTLSDE 94

Query: 82  NGDI-------GITWEFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTA 141
            G++          W+FNFR F+ ISDM  + S++  L+    D EK    G+  + F  
Sbjct: 95  KGNLPTCGTNKQCIWQFNFREFNVISDMFALDSIE-LLRKSAIDLEKNNECGVDAKRFAE 154

Query: 142 LFL--PICRSGRIDRWITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKY 190
           L +   +  + +I  W+TFH  YD  YL+KLL+ + +   ++ F    ++    + D+KY
Sbjct: 155 LLMGSGVVLNDKI-HWVTFHCGYDFGYLLKLLSGKELPEEISDFFDQMEKFFPVVYDIKY 214

[Close]

BLAST of Lag0032731 vs. ExPASy TrEMBL
Match: A0A6J1DLA5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 3.4e-70
Identity = 137/199 (68.84%), Postives = 150/199 (75.38%), Query Frame = 0


Query: 20  EYLPEFPIVAIDTEF-----------------------------PGFLRYAPKGVSEEEF 79
           EYLPEFPIVAIDTEF                             PGFLRY PKGVSEEEF
Sbjct: 35  EYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEF 94

Query: 80  YECLRFNVNRLSILQLGLTLTNQNGDIGITWEFNFRGFDRISDMEGVSSLDSFLKNKGFD 139
           Y+CLRFNVNRL+ILQLGLTL NQNG++ +TWEFNFR FD ISDMEGVSSLDSFLKNKGFD
Sbjct: 95  YDCLRFNVNRLNILQLGLTLANQNGNVAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFD 154

Query: 140 FEKLRNDGIAPEEFTALFLPICRSGRIDRWITFHGSYDIAYLMKLLNIRSMATSMAIFAA 190
           F KL+ DGIA E+FT LFLPICRSGRI+RWITFHG YDIAYL+KLL I+ +  SMA FAA
Sbjct: 155 FYKLKRDGIALEDFTTLFLPICRSGRIERWITFHGFYDIAYLLKLLKIKYIPISMATFAA 214

[Close]

BLAST of Lag0032731 vs. ExPASy TrEMBL
Match: A0A6J1CXZ5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.6e-67
Identity = 125/170 (73.53%), Postives = 140/170 (82.35%), Query Frame = 0


Query: 20  EYLPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGI 79
           EYLPEFP+V ID EFPGFL   PKG+SEEEFY CLRFNVNRL+ILQLGLTL +Q G+I +
Sbjct: 54  EYLPEFPVVVIDMEFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAM 113

Query: 80  TWEFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLPICRSGRIDR 139
           TWEFNFR FD +SDME VSSLDSFLKNKGFDF KL+  GIA E+FT LFLPICRSG+I R
Sbjct: 114 TWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYKLKKYGIAQEDFTTLFLPICRSGQIKR 173

Query: 140 WITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYMPRRC 190
           WITFHG YDI YL+KLL I+S+  SMA FA  AQ LLGT+ DLK+M R C
Sbjct: 174 WITFHGFYDITYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYC 223

[Close]

BLAST of Lag0032731 vs. ExPASy TrEMBL
Match: A0A6J1HL76 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.6e-67
Identity = 127/164 (77.44%), Postives = 139/164 (84.76%), Query Frame = 0


Query: 22  LPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGITW 81
           L EFPIVAIDTEFPGFLR+ PKGVS+EEFYECL+FNVNRLSILQLGLTLTN NG IG+TW
Sbjct: 35  LSEFPIVAIDTEFPGFLRWTPKGVSDEEFYECLQFNVNRLSILQLGLTLTNDNGKIGMTW 94

Query: 82  EFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLPICRSGRIDRWI 141
           EFNFR FD+IS+MEGV SLDSFLKNKGF+F  LR+ GIAPEEF   F PI RSGRI RWI
Sbjct: 95  EFNFRDFDKISNMEGVYSLDSFLKNKGFNFNTLRSYGIAPEEFGTQFSPILRSGRIHRWI 154

Query: 142 TFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYM 186
           TFHG YDIAYLMK  NIR M TSM+IFA  A  +LGT+ DLK+M
Sbjct: 155 TFHGLYDIAYLMKFTNIRCMPTSMSIFAITAAHILGTVTDLKHM 198

[Close]

BLAST of Lag0032731 vs. ExPASy TrEMBL
Match: A0A6J1HIZ7 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463978 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.3e-66
Identity = 127/166 (76.51%), Postives = 140/166 (84.34%), Query Frame = 0


Query: 22  LPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGITW 81
           L EFPIVA+DTEFPGFLR+ PKGVS+EEFYECL+FNVNRLSILQLGLTLTN NG IG+TW
Sbjct: 32  LSEFPIVAMDTEFPGFLRWTPKGVSDEEFYECLQFNVNRLSILQLGLTLTNDNGKIGMTW 91

Query: 82  EFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLPICRSGRIDRWI 141
           EFNF+ FD+IS+MEGV SLDSFLKNKGF+F  LR+ GIAPEEF   F PI RSGRI RWI
Sbjct: 92  EFNFKDFDKISNMEGVYSLDSFLKNKGFNFNTLRSYGIAPEEFGRQFNPILRSGRIHRWI 151

Query: 142 TFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYMPR 188
           TFHG YDIAYLMKL NI  M TSM+IFA  A  LLGT+ DLK+M R
Sbjct: 152 TFHGLYDIAYLMKLTNITCMPTSMSIFAITAAHLLGTVTDLKHMAR 197

[Close]

BLAST of Lag0032731 vs. ExPASy TrEMBL
Match: A0A6J1ESM7 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 5.0e-66
Identity = 124/167 (74.25%), Postives = 143/167 (85.63%), Query Frame = 0


Query: 21  YLPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGIT 80
           YL EFPIVAIDTEFPGFLR+ PKGVS+EEFY+CL+FNVNRLSILQLGLTLT+ NG IG+T
Sbjct: 34  YLSEFPIVAIDTEFPGFLRWTPKGVSDEEFYQCLQFNVNRLSILQLGLTLTDDNGKIGMT 93

Query: 81  WEFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLPICRSGRIDRW 140
           WEFNF+ FD+IS+MEGV SLDSFLKNKGF+F KLR+ GIAP+EF+  F P+ RSGRI +W
Sbjct: 94  WEFNFQDFDQISNMEGVYSLDSFLKNKGFNFNKLRSCGIAPQEFSRQFKPVIRSGRIQKW 153

Query: 141 ITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYMPR 188
           ITFHG YDIAYLMK  NI SM TSM++FA  A  LLGT+ DLK+M R
Sbjct: 154 ITFHGLYDIAYLMKFNNIISMPTSMSMFAMIAAHLLGTVTDLKHMAR 200

[Close]

BLAST of Lag0032731 vs. TAIR 10
Match: AT3G44240.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 128.3 bits (321), Expect = 7.5e-30
Identity = 68/170 (40.00%), Postives = 101/170 (59.41%), Query Frame = 0


Query: 22  LPEFPIVAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGITW 81
           L  +  +AIDTEFP  LR   +  ++EE Y  + F+V+R  ++QLGLTL + NG IG TW
Sbjct: 8   LRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRIGGTW 67

Query: 82  EFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEE-FTALFLPICRSGRIDRW 141
           E NF  F  + D     S++ FL+  G D  K+R +GI  E  F+ +F  + ++ R   W
Sbjct: 68  EINFSDFG-VDDARNEKSIE-FLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRNITW 127

Query: 142 ITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYMPRRCK 191
           +TFHGSYDIAYL+K     ++  +   F+    ++LG++ DLK M  RC+
Sbjct: 128 VTFHGSYDIAYLLKGFTGEALPVTSERFSKAVARVLGSVYDLKVMAGRCE 175

[Close]

BLAST of Lag0032731 vs. TAIR 10
Match: AT1G61470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 7.8e-27
Identity = 69/165 (41.82%), Postives = 95/165 (57.58%), Query Frame = 0


Query: 28  VAIDTEFPGFLRYAPKGVSEEEFYECLRFNVNRLSILQLGLTLTNQNGDIGITWEFNFRG 87
           +AIDTEFPG L+  P   S+E  Y  ++FNV+   ++QLGLTL  +   I  TWE N   
Sbjct: 27  IAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLIQLGLTLFGKG--ITKTWEINLSD 86

Query: 88  FDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFLPIC--RSGRIDRWITFHG 147
           F+    ++   S+ +FLKN G D +K+R +GI  EEF   F  I   + G++ RW+TF G
Sbjct: 87  FNESKSLKNDKSI-AFLKNNGLDLDKIREEGIGIEEFFMEFSQILNEKHGKM-RWVTFQG 146

Query: 148 SYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIV-DLKYMPRRC 190
           SYD AYL+K L  + +  +   F    QQLLG  V D+K M   C
Sbjct: 147 SYDKAYLLKGLTRKPLPETSKEFDETVQQLLGRFVYDVKKMAGLC 187

[Close]

BLAST of Lag0032731 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.8e-24
Identity = 65/184 (35.33%), Postives = 97/184 (52.72%), Query Frame = 0


Query: 20  EYLPEFPIVAIDTEFPGFLRYAPKGV---SEEEFYECLRFNVNRLSILQLGLTLTNQNGD 79
           + + +FP VA+DTEFPG +   P G    + +  YE L+ NVN L ++QLGLT +N+ G+
Sbjct: 29  DVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVNILKMIQLGLTFSNEQGN 88

Query: 80  IG-------ITWEFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFL 139
           +          W+FNFR FD  SD+  + S++  LK  G D  K   DGI  + F  L +
Sbjct: 89  LPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKNTLDGIDSKRFAELLM 148

Query: 140 PICRSGRI----DRWITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYM 190
               SG +      W+TFH  YD  YL+KLL  +++  S   F         T+ D+K++
Sbjct: 149 ---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLINVYFPTVYDIKHL 207

[Close]

BLAST of Lag0032731 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.8e-24
Identity = 65/184 (35.33%), Postives = 97/184 (52.72%), Query Frame = 0


Query: 20  EYLPEFPIVAIDTEFPGFLRYAPKGV---SEEEFYECLRFNVNRLSILQLGLTLTNQNGD 79
           + + +FP VA+DTEFPG +   P G    + +  YE L+ NVN L ++QLGLT +N+ G+
Sbjct: 29  DVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVNILKMIQLGLTFSNEQGN 88

Query: 80  IG-------ITWEFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFL 139
           +          W+FNFR FD  SD+  + S++  LK  G D  K   DGI  + F  L +
Sbjct: 89  LPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKNTLDGIDSKRFAELLM 148

Query: 140 PICRSGRI----DRWITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYM 190
               SG +      W+TFH  YD  YL+KLL  +++  S   F         T+ D+K++
Sbjct: 149 ---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLINVYFPTVYDIKHL 207

[Close]

BLAST of Lag0032731 vs. TAIR 10
Match: AT1G80780.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.8e-24
Identity = 65/184 (35.33%), Postives = 97/184 (52.72%), Query Frame = 0


Query: 20  EYLPEFPIVAIDTEFPGFLRYAPKGV---SEEEFYECLRFNVNRLSILQLGLTLTNQNGD 79
           + + +FP VA+DTEFPG +   P G    + +  YE L+ NVN L ++QLGLT +N+ G+
Sbjct: 29  DVVDDFPYVAMDTEFPGIV-VRPVGTFKSNADYHYETLKTNVNILKMIQLGLTFSNEQGN 88

Query: 80  IG-------ITWEFNFRGFDRISDMEGVSSLDSFLKNKGFDFEKLRNDGIAPEEFTALFL 139
           +          W+FNFR FD  SD+  + S++  LK  G D  K   DGI  + F  L +
Sbjct: 89  LPTCGTDKYCIWQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKNTLDGIDSKRFAELLM 148

Query: 140 PICRSGRI----DRWITFHGSYDIAYLMKLLNIRSMATSMAIFAANAQQLLGTIVDLKYM 190
               SG +      W+TFH  YD  YL+KLL  +++  S   F         T+ D+K++
Sbjct: 149 ---SSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLINVYFPTVYDIKHL 207

The following BLAST results are available for this feature:

BLAST of Lag0032731 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154798.1	7.0e-70	68.84	probable CCR4-associated factor 1 homolog 6 [Momordica charantia]	[more]
XP_022146384.1	3.2e-67	73.53	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]
XP_022963784.1	9.4e-67	77.44	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]
XP_022963785.1	2.7e-66	76.51	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]
XP_022928830.1	1.0e-65	74.25	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]

BLAST of Lag0032731 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXM4	1.1e-28	40.00	Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
O64773	1.1e-25	41.82	Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SAI2	3.9e-23	35.33	Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SHJ0	5.1e-23	33.52	Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9S9P2	1.9e-22	32.43	Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]

BLAST of Lag0032731 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLA5	3.4e-70	68.84	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=...	[more]
A0A6J1CXZ5	1.6e-67	73.53	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=...	[more]
A0A6J1HL76	4.6e-67	77.44	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3...	[more]
A0A6J1HIZ7	1.3e-66	76.51	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463978 PE=3...	[more]
A0A6J1ESM7	5.0e-66	74.25	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3...	[more]

BLAST of Lag0032731 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G44240.1	7.5e-30	40.00	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G61470.1	7.8e-27	41.82	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.1	2.8e-24	35.33	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.2	2.8e-24	35.33	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.3	2.8e-24	35.33	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006941	Ribonuclease CAF1	PFAM	PF04857	CAF1	coord: 28..119 e-value: 3.1E-8 score: 33.3
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 19..209 e-value: 7.9E-45 score: 154.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 191..211
None	No IPR available	PANTHER	PTHR10797:SF57	CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATED	coord: 21..191
IPR039637	CCR4-NOT transcription complex subunit 7/8/Pop2	PANTHER	PTHR10797	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT	coord: 21..191
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 20..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0032731.1	Lag0032731.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043928	exonucleolytic catabolism of deadenylated mRNA
biological_process	GO:0090503	RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component	GO:0030015	CCR4-NOT core complex
cellular_component	GO:0005634	nucleus
cellular_component	GO:0000932	P-body
cellular_component	GO:0030014	CCR4-NOT complex
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity