Lag0032690 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032690
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionheavy metal-associated isoprenylated plant protein 39-like
Locationchr11: 36281108 .. 36281706 (-)
RNA-Seq ExpressionLag0032690
SyntenyLag0032690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGTAATTAATTAATTAATTAATTCCCCTCGTTCCCTCTTCCCACAAAAAAAAAAATTATGGTATTTTTATTTTATTTTTTAAACAAATTAAAAGCAATTAATTAATGGGTTTCTTTCTAATTTGCAGAAACTTGTGCTGAAAGTGGAAATCAGCGATGCAAAGACAAAGAAAAAGGTGTTGAGTGCTGTTTCTGGCCTTTTGGGTAACATCAACAAACTGGGTCTTCTTTAATTTTCAGTACTATTGATATTAAAATTAACCCCCAAAAAGAAAAAAATATATATATTTTTTTTAATTATTATATAGGGTGATACTGATGTGGGTTTTGGGAAAAACTTGCAGGAATTGTGTCAATGGCAACAGACACAAAAGAGAACAAAATAACAGTGATTGGAACTGCAGACCCAATTAAGATTGTTGGGAAAGTAAGAAAGATTTGGCCTTCAGCTTACATTATTAGCATTGGAGAAGAGAAATCAGAAGAGAAGAAGAAAGAAGAAGATACTACACCCAAAATTGAGCAGATTGATTGGTTTAAGGCTTACACAGCCTTCCCTTCTTACCCCACAACTTATTACTATCATCCCTATTAA

mRNA sequence

ATGATGAAACTTGTGCTGAAAGTGGAAATCAGCGATGCAAAGACAAAGAAAAAGGTGTTGAGTGCTGTTTCTGGCCTTTTGGGAATTGTGTCAATGGCAACAGACACAAAAGAGAACAAAATAACAGTGATTGGAACTGCAGACCCAATTAAGATTGTTGGGAAAGTAAGAAAGATTTGGCCTTCAGCTTACATTATTAGCATTGGAGAAGAGAAATCAGAAGAGAAGAAGAAAGAAGAAGATACTACACCCAAAATTGAGCAGATTGATTGGTTTAAGGCTTACACAGCCTTCCCTTCTTACCCCACAACTTATTACTATCATCCCTATTAA

Coding sequence (CDS)

ATGATGAAACTTGTGCTGAAAGTGGAAATCAGCGATGCAAAGACAAAGAAAAAGGTGTTGAGTGCTGTTTCTGGCCTTTTGGGAATTGTGTCAATGGCAACAGACACAAAAGAGAACAAAATAACAGTGATTGGAACTGCAGACCCAATTAAGATTGTTGGGAAAGTAAGAAAGATTTGGCCTTCAGCTTACATTATTAGCATTGGAGAAGAGAAATCAGAAGAGAAGAAGAAAGAAGAAGATACTACACCCAAAATTGAGCAGATTGATTGGTTTAAGGCTTACACAGCCTTCCCTTCTTACCCCACAACTTATTACTATCATCCCTATTAA

Protein sequence

MMKLVLKVEISDAKTKKKVLSAVSGLLGIVSMATDTKENKITVIGTADPIKIVGKVRKIWPSAYIISIGEEKSEEKKKEEDTTPKIEQIDWFKAYTAFPSYPTTYYYHPY
Homology
BLAST of Lag0032690 vs. NCBI nr
Match: XP_038875236.1 (heavy metal-associated isoprenylated plant protein 39-like [Benincasa hispida])

HSP 1 Score: 159.8 bits (403), Expect = 1.3e-35
Identity = 84/113 (74.34%), Postives = 96/113 (84.96%), Query Frame = 0

Query: 1   MMKLVLKVEISDAKTKKKVLSAVSGLLGIVSMATDTKENKITVIGTADPIKIVGKVRKIW 60
           MMK+VLKVEI DAKTK+KV+  VSGLLGIVS+A DTKENKITVIGTADPIKIV KVRK+W
Sbjct: 1   MMKVVLKVEICDAKTKRKVMGTVSGLLGIVSVAADTKENKITVIGTADPIKIVKKVRKVW 60

Query: 61  PSAYIISIGEEKSEEKK---KEEDTTPKIEQIDWFKAYTAFPSYPTTYYYHPY 111
           PSAY+IS+G EK E KK   K+E+  PKI+QIDW KA  AF SYPTT+YY+PY
Sbjct: 61  PSAYLISVGPEKEEAKKPDPKKEEAQPKIDQIDWVKACNAFHSYPTTHYYYPY 113

BLAST of Lag0032690 vs. NCBI nr
Match: KAG7016848.1 (Heavy metal-associated isoprenylated plant protein 39, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 158.7 bits (400), Expect = 2.9e-35
Identity = 87/112 (77.68%), Postives = 95/112 (84.82%), Query Frame = 0

Query: 1   MMKLVLKVEISDAKTKKKVLSAVSGLLGIVSMATDTKENKITVIGTADPIKIVGKVRKIW 60
           M K+VLKVEISD+KTKKKVLSAVSGL GIVSMATDTKENKITVIGTADPI+IV KVRKIW
Sbjct: 1   MAKVVLKVEISDSKTKKKVLSAVSGLSGIVSMATDTKENKITVIGTADPIEIVKKVRKIW 60

Query: 61  PSAYIISIGEEKSE----EKKKEEDTTPKIEQIDWFKAYTAFPSYPTTYYYH 109
           PSAYIIS+G EK E    E KK++  TPKIE IDW KAYTA  SYPT +YY+
Sbjct: 61  PSAYIISVGPEKEEPKKVEPKKQDPPTPKIEPIDWAKAYTALQSYPTPHYYY 112

BLAST of Lag0032690 vs. NCBI nr
Match: KAG6579362.1 (Heavy metal-associated isoprenylated plant protein 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 157.9 bits (398), Expect = 5.0e-35
Identity = 86/110 (78.18%), Postives = 94/110 (85.45%), Query Frame = 0

Query: 3   KLVLKVEISDAKTKKKVLSAVSGLLGIVSMATDTKENKITVIGTADPIKIVGKVRKIWPS 62
           K+VLKVEISD+KTKKKVLSAVSGL GIVSMATDTKENKITVIGTADPI+IV KVRKIWPS
Sbjct: 33  KVVLKVEISDSKTKKKVLSAVSGLSGIVSMATDTKENKITVIGTADPIEIVKKVRKIWPS 92

Query: 63  AYIISIGEEKSE----EKKKEEDTTPKIEQIDWFKAYTAFPSYPTTYYYH 109
           AYIIS+G EK E    E KK++  TPKIE IDW KAYTA  SYPT +YY+
Sbjct: 93  AYIISVGPEKEEPKKVEPKKQDPPTPKIEPIDWAKAYTALQSYPTPHYYY 142

BLAST of Lag0032690 vs. NCBI nr
Match: XP_022922259.1 (heavy metal-associated isoprenylated plant protein 39-like [Cucurbita moschata])

HSP 1 Score: 156.8 bits (395), Expect = 1.1e-34
Identity = 86/112 (76.79%), Postives = 94/112 (83.93%), Query Frame = 0

Query: 1   MMKLVLKVEISDAKTKKKVLSAVSGLLGIVSMATDTKENKITVIGTADPIKIVGKVRKIW 60
           M K+VLKVEISD+KTKKKVLSAVSGL GIVSMATD KENKITVIGTADPI+IV KVRKIW
Sbjct: 1   MAKVVLKVEISDSKTKKKVLSAVSGLSGIVSMATDAKENKITVIGTADPIEIVKKVRKIW 60

Query: 61  PSAYIISIGEEKSE----EKKKEEDTTPKIEQIDWFKAYTAFPSYPTTYYYH 109
           PSAYIIS+G EK E    E KK++  TPKIE IDW KAYTA  SYPT +YY+
Sbjct: 61  PSAYIISVGPEKEEPKKVEPKKQDPPTPKIEPIDWAKAYTALQSYPTPHYYY 112

BLAST of Lag0032690 vs. NCBI nr
Match: XP_023551446.1 (heavy metal-associated isoprenylated plant protein 39-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 156.4 bits (394), Expect = 1.4e-34
Identity = 85/112 (75.89%), Postives = 95/112 (84.82%), Query Frame = 0

Query: 1   MMKLVLKVEISDAKTKKKVLSAVSGLLGIVSMATDTKENKITVIGTADPIKIVGKVRKIW 60
           M K+VLKVEI+D+KTKKKVLSAVSGL GIVSMATDTK+NKITVIGTADPI+IV KVRKIW
Sbjct: 1   MAKVVLKVEINDSKTKKKVLSAVSGLSGIVSMATDTKDNKITVIGTADPIEIVKKVRKIW 60

Query: 61  PSAYIISIGEEKSE----EKKKEEDTTPKIEQIDWFKAYTAFPSYPTTYYYH 109
           PSAYIIS+G EK E    E KK++  TPKIE IDW KAYTA  SYPT +YY+
Sbjct: 61  PSAYIISVGPEKEEPKKVEPKKQDPPTPKIEPIDWAKAYTALQSYPTPHYYY 112

BLAST of Lag0032690 vs. ExPASy Swiss-Prot
Match: O03982 (Heavy metal-associated isoprenylated plant protein 39 OS=Arabidopsis thaliana OX=3702 GN=HIPP39 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 2.9e-14
Identity = 43/87 (49.43%), Postives = 59/87 (67.82%), Query Frame = 0

Query: 1  MMKLVLKVEISDAKTKKKVLSAVSGLLGIVSMATDTKENKITVIGTADPIKIVGKVRKIW 60
          M K+VLK+++ D + K+K L  VS L GI S+A D KE K+TVIGT DP+ +V K+RK W
Sbjct: 1  MKKIVLKLDLHDDRAKQKALKTVSTLPGIDSIAMDMKEKKLTVIGTVDPVNVVSKLRKYW 60

Query: 61 PSAYIISIGEEKSEEKKKEEDTTPKIE 88
          P   I+ +G  K  EK+K+E+  PK E
Sbjct: 61 PMTDIVLVGPAKEPEKEKKEE--PKKE 85

BLAST of Lag0032690 vs. ExPASy Swiss-Prot
Match: Q9LTE1 (Heavy metal-associated isoprenylated plant protein 14 OS=Arabidopsis thaliana OX=3702 GN=HIPP14 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 7.3e-05
Identity = 35/108 (32.41%), Postives = 58/108 (53.70%), Query Frame = 0

Query: 5   VLKVEISDAKTKKKVLSAVSGLLGIVSMATDTKENKITVIGTADPIKIVGKVRKIWPSAY 64
           VL++ I + +T+KK L  VS   G+ S+  D K  K+T++G  D   +V K+RK+  +  
Sbjct: 7   VLQLSIHEERTRKKALVTVSRFSGVTSITMD-KSGKMTIVGEVDVPAVVMKLRKLC-NTE 66

Query: 65  IISIGEEKSEEKKKEEDTTPK--IEQIDWFKAYTAFPSYPTTYYYHPY 111
           I+S+ + K   KK E +   +     +    AY   P+Y  +YY+ PY
Sbjct: 67  IVSVDDVKPPVKKPEPEKPAESIAYPVPMNYAYQFNPAYANSYYHQPY 112

BLAST of Lag0032690 vs. ExPASy Swiss-Prot
Match: Q9LTE3 (Heavy metal-associated isoprenylated plant protein 12 OS=Arabidopsis thaliana OX=3702 GN=HIPP12 PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 9.5e-05
Identity = 40/111 (36.04%), Postives = 60/111 (54.05%), Query Frame = 0

Query: 1   MMKLVLKVEISDAKTKKKVLSAVSGLLGIVSMATDTKENKITVIGTADPIKIVGKVRKIW 60
           M  +VLK+++   KTK+K +S V  L G+ S+  + K+ K+TV G  D   IV K++KI 
Sbjct: 1   MQVVVLKLDVHCEKTKQKAMSTVCCLSGVNSV--EVKDGKLTVTGEIDAYMIVKKLKKIC 60

Query: 61  PSAYIISIGEEKSEEKKKEED-TTPKIEQIDWFKAYTAFPSYPTTYYYHPY 111
            + + IS+G  K  EKKK +D   P+ +  D    Y      P   YYH +
Sbjct: 61  HTEF-ISVGPVKEPEKKKPDDPKKPETKPPDVIYHYVP----PCPPYYHNF 104

BLAST of Lag0032690 vs. ExPASy Swiss-Prot
Match: Q8GWS3 (Heavy metal-associated isoprenylated plant protein 2 OS=Arabidopsis thaliana OX=3702 GN=HIPP02 PE=3 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.6e-04
Identity = 25/82 (30.49%), Postives = 48/82 (58.54%), Query Frame = 0

Query: 3  KLVLKVEISDAKTKKKVLSAVSGLLGIVSMATDTKENKITVIGTADPIKIVGKVRKIWPS 62
          K+ +KV+I   K K  ++ AV+ L G+  ++ D +++ +TV+GT DP+ +  +++KI   
Sbjct: 5  KIEIKVDIDCEKCKHAIMEAVTELEGVNIVSLDQEKSILTVVGTMDPVCVAEQLKKINKK 64

Query: 63 AYIISIGEEKSEEKKKEEDTTP 85
            +IS+G  K  + K +    P
Sbjct: 65 PVVISVGPPKKPDPKPDPKKDP 86

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875236.11.3e-3574.34heavy metal-associated isoprenylated plant protein 39-like [Benincasa hispida][more]
KAG7016848.12.9e-3577.68Heavy metal-associated isoprenylated plant protein 39, partial [Cucurbita argyro... [more]
KAG6579362.15.0e-3578.18Heavy metal-associated isoprenylated plant protein 39, partial [Cucurbita argyro... [more]
XP_022922259.11.1e-3476.79heavy metal-associated isoprenylated plant protein 39-like [Cucurbita moschata][more]
XP_023551446.11.4e-3475.89heavy metal-associated isoprenylated plant protein 39-like [Cucurbita pepo subsp... [more]
Match NameE-valueIdentityDescription
O039822.9e-1449.43Heavy metal-associated isoprenylated plant protein 39 OS=Arabidopsis thaliana OX... [more]
Q9LTE17.3e-0532.41Heavy metal-associated isoprenylated plant protein 14 OS=Arabidopsis thaliana OX... [more]
Q9LTE39.5e-0536.04Heavy metal-associated isoprenylated plant protein 12 OS=Arabidopsis thaliana OX... [more]
Q8GWS31.6e-0430.49Heavy metal-associated isoprenylated plant protein 2 OS=Arabidopsis thaliana OX=... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.100coord: 1..74
e-value: 5.3E-17
score: 63.7
NoneNo IPR availablePANTHERPTHR45811COPPER TRANSPORT PROTEIN FAMILY-RELATEDcoord: 1..107
NoneNo IPR availablePANTHERPTHR45811:SF49COPPER TRANSPORT PROTEIN FAMILY-RELATEDcoord: 1..107
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 7..60
e-value: 9.7E-6
score: 25.9
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 1..69

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032690.1Lag0032690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding