Lag0032673 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0032673
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	chr11: 36038476 .. 36040277 (+)
RNA-Seq Expression	Lag0032673
Synteny	Lag0032673

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAAAACTTTTCCAGCTACTCTCTTTCTAGCATTAGTCTTTATGTTTTCATTTCTTTCAACTCATTCACTTCCCTTGTCAACAAGAGGAAGATGGATCATCGATTCAAAAACAGGAAATCGAGTGAAGCTTGTGTGTGTGAATTGGCCTTCTCACACACAGAGCATGCTGATAGAAGGACTCAACCACAGGCCATTGAAAGAGCTTGCAGATGAGGTAGTCAAGTTGAGATTCAATTGTGTGCGACTCACATATGCAACCCATATGTTTACTCGCTATGCAAATAGGACAGTTGCAGAGAACTTCGACCTTCTCGATTTAGAACAAGCAAAAGCTGGATTGGCTCAGTATAATCCTTTTGTGCTAAACAAGACTATTGCTGAAGCCTATGAAGCAGTTGTTGATGTGCTTGGGGCGAGTGGTTTAATGGTAGTTGCTGACAACCATATGAGCCAGCCAAGATGGTGTTGCTCTCTTGACGATGGCAATGGTTTCTTTGGAGATCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCCCAACGCTTTATCAACAATCCAACGGTATGTAATACATCAAAAGATTTTCTCAATTCTTGAAACTAAAATAAATCATTTTAAAGACTTCAACAATATTTCATATTACCAAAATAATTTTTTTATGGCATTTAGGTGGTGGCAATGAGCCTACGGAATGAGATACGAGGAGCGATGGAAAATGCAATTGATTGGAACAAGTATGTAACTCAAGGAGTAACAACGATCCACAAGATTAACCCAGATGTTTTAGTGGTTGTTTCCGGCCTAAATTATGATAATGATCTTCGATGCCTAAAGGAGAAGCCTTTGACCGTAAACACCTTAGACAACAAGTTGGTTTTCGAGGTGCACCTATATTCCTTCAGTGGAGATTCCGAGACTAAATTTGTACAACAACCACTAAACAATATATGTGCAAATATTATCAATGGCTTCGAAGACCATGCTGGGTTTGTAATGGAAGGACCAAGTCCATTTCCTTTGTTTGTCAGTGAATATGGGTATGATCAAAGAGAGGTTGACGAGGCAGAAAACCGATTCATGAGTTGCTTCATGGCTCATCTTGCACAAAAGGATTTGGATTGGGCATTGTGGACTTGGCAAGGTAGTTATTATTACAGGGAAGGTAATGCAGAGCCTGCAGAGACTTTTGGAGTTCTCGAATCTAACTGGACTCAAATTAAAAACCCTAACTTTGTCCAGAAGTTTCAACTGTTGCAGACCATGTTGCAAGGTAACTATGTATATTAGTGATTTTGAATACATGTTAGAGTAGTCCATAACAATTTGGACAATTTTTCTTATACTATGCAGATCCAAATTCCAATGCTTCATTCTCGTATGTTATATATCATCCACAAAGTGGCCAATGTGTCCATGTCTCCAACGACAACAAAGAAATTTTCCTCAACAATTGCTCTGCCTCAAGTCGATGGAGTCATGACAATGATAGCACTCCAATTAAGATGTCAAGCACTGGCCTATGCTTAAAGGCTAATGGAGAAGGCCTCGAGACATCTCTCTCAACTGACTGCTCAGGCAAACAAAGTGTTTGGACAGCCATTTCAAACACTAAGCTTCATTTGGCCACCTTCACTCAAGATGGAAAGAACCTTTGCTTACAGATTGCAAGTTCTAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGTACCAATGGCGAGCCAAATTGCCTCCAAGACACCCAAAGTCAATGGTTTGAACTTGTTGGAACCAACACAATCTGA

mRNA sequence

ATGGGAAAAACTTTTCCAGCTACTCTCTTTCTAGCATTAGTCTTTATGTTTTCATTTCTTTCAACTCATTCACTTCCCTTGTCAACAAGAGGAAGATGGATCATCGATTCAAAAACAGGAAATCGAGTGAAGCTTGTGTGTGTGAATTGGCCTTCTCACACACAGAGCATGCTGATAGAAGGACTCAACCACAGGCCATTGAAAGAGCTTGCAGATGAGGTAGTCAAGTTGAGATTCAATTGTGTGCGACTCACATATGCAACCCATATGTTTACTCGCTATGCAAATAGGACAGTTGCAGAGAACTTCGACCTTCTCGATTTAGAACAAGCAAAAGCTGGATTGGCTCAGTATAATCCTTTTGTGCTAAACAAGACTATTGCTGAAGCCTATGAAGCAGTTGTTGATGTGCTTGGGGCGAGTGGTTTAATGGTAGTTGCTGACAACCATATGAGCCAGCCAAGATGGTGTTGCTCTCTTGACGATGGCAATGGTTTCTTTGGAGATCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCCCAACGCTTTATCAACAATCCAACGGTGGTGGCAATGAGCCTACGGAATGAGATACGAGGAGCGATGGAAAATGCAATTGATTGGAACAAGTATGTAACTCAAGGAGTAACAACGATCCACAAGATTAACCCAGATGTTTTAGTGGTTGTTTCCGGCCTAAATTATGATAATGATCTTCGATGCCTAAAGGAGAAGCCTTTGACCGTAAACACCTTAGACAACAAGTTGGTTTTCGAGGTGCACCTATATTCCTTCAGTGGAGATTCCGAGACTAAATTTGTACAACAACCACTAAACAATATATGTGCAAATATTATCAATGGCTTCGAAGACCATGCTGGGTTTGTAATGGAAGGACCAAGTCCATTTCCTTTGTTTGTCAGTGAATATGGGTATGATCAAAGAGAGGTTGACGAGGCAGAAAACCGATTCATGAGTTGCTTCATGGCTCATCTTGCACAAAAGGATTTGGATTGGGCATTGTGGACTTGGCAAGGTAGTTATTATTACAGGGAAGGTAATGCAGAGCCTGCAGAGACTTTTGGAGTTCTCGAATCTAACTGGACTCAAATTAAAAACCCTAACTTTGTCCAGAAGTTTCAACTGTTGCAGACCATGTTGCAAGATCCAAATTCCAATGCTTCATTCTCGTATGTTATATATCATCCACAAAGTGGCCAATGTGTCCATGTCTCCAACGACAACAAAGAAATTTTCCTCAACAATTGCTCTGCCTCAAGTCGATGGAGTCATGACAATGATAGCACTCCAATTAAGATGTCAAGCACTGGCCTATGCTTAAAGGCTAATGGAGAAGGCCTCGAGACATCTCTCTCAACTGACTGCTCAGGCAAACAAAGTGTTTGGACAGCCATTTCAAACACTAAGCTTCATTTGGCCACCTTCACTCAAGATGGAAAGAACCTTTGCTTACAGATTGCAAGTTCTAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGTACCAATGGCGAGCCAAATTGCCTCCAAGACACCCAAAGTCAATGGTTTGAACTTGTTGGAACCAACACAATCTGA

Coding sequence (CDS)

ATGGGAAAAACTTTTCCAGCTACTCTCTTTCTAGCATTAGTCTTTATGTTTTCATTTCTTTCAACTCATTCACTTCCCTTGTCAACAAGAGGAAGATGGATCATCGATTCAAAAACAGGAAATCGAGTGAAGCTTGTGTGTGTGAATTGGCCTTCTCACACACAGAGCATGCTGATAGAAGGACTCAACCACAGGCCATTGAAAGAGCTTGCAGATGAGGTAGTCAAGTTGAGATTCAATTGTGTGCGACTCACATATGCAACCCATATGTTTACTCGCTATGCAAATAGGACAGTTGCAGAGAACTTCGACCTTCTCGATTTAGAACAAGCAAAAGCTGGATTGGCTCAGTATAATCCTTTTGTGCTAAACAAGACTATTGCTGAAGCCTATGAAGCAGTTGTTGATGTGCTTGGGGCGAGTGGTTTAATGGTAGTTGCTGACAACCATATGAGCCAGCCAAGATGGTGTTGCTCTCTTGACGATGGCAATGGTTTCTTTGGAGATCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCCCAACGCTTTATCAACAATCCAACGGTGGTGGCAATGAGCCTACGGAATGAGATACGAGGAGCGATGGAAAATGCAATTGATTGGAACAAGTATGTAACTCAAGGAGTAACAACGATCCACAAGATTAACCCAGATGTTTTAGTGGTTGTTTCCGGCCTAAATTATGATAATGATCTTCGATGCCTAAAGGAGAAGCCTTTGACCGTAAACACCTTAGACAACAAGTTGGTTTTCGAGGTGCACCTATATTCCTTCAGTGGAGATTCCGAGACTAAATTTGTACAACAACCACTAAACAATATATGTGCAAATATTATCAATGGCTTCGAAGACCATGCTGGGTTTGTAATGGAAGGACCAAGTCCATTTCCTTTGTTTGTCAGTGAATATGGGTATGATCAAAGAGAGGTTGACGAGGCAGAAAACCGATTCATGAGTTGCTTCATGGCTCATCTTGCACAAAAGGATTTGGATTGGGCATTGTGGACTTGGCAAGGTAGTTATTATTACAGGGAAGGTAATGCAGAGCCTGCAGAGACTTTTGGAGTTCTCGAATCTAACTGGACTCAAATTAAAAACCCTAACTTTGTCCAGAAGTTTCAACTGTTGCAGACCATGTTGCAAGATCCAAATTCCAATGCTTCATTCTCGTATGTTATATATCATCCACAAAGTGGCCAATGTGTCCATGTCTCCAACGACAACAAAGAAATTTTCCTCAACAATTGCTCTGCCTCAAGTCGATGGAGTCATGACAATGATAGCACTCCAATTAAGATGTCAAGCACTGGCCTATGCTTAAAGGCTAATGGAGAAGGCCTCGAGACATCTCTCTCAACTGACTGCTCAGGCAAACAAAGTGTTTGGACAGCCATTTCAAACACTAAGCTTCATTTGGCCACCTTCACTCAAGATGGAAAGAACCTTTGCTTACAGATTGCAAGTTCTAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGTACCAATGGCGAGCCAAATTGCCTCCAAGACACCCAAAGTCAATGGTTTGAACTTGTTGGAACCAACACAATCTGA

Protein sequence

MGKTFPATLFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEVVKLRFNCVRLTYATHMFTRYANRTVAENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNYDNDLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGFVMEGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNAEPAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVSNDNKEIFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANGEGLETSLSTDCSGKQSVWTAISNTKLHLATFTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELVGTNTI

Homology

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BLAST of Lag0032673 vs. NCBI nr
Match: KAE8646606.1 (hypothetical protein Csa_005432 [Cucumis sativus])

HSP 1 Score: 961.8 bits (2485), Expect = 2.4e-276
Identity = 463/538 (86.06%), Postives = 491/538 (91.26%), Query Frame = 0


Query: 1   MGKTFPATLFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIE 60
           M +T    L LALV +FS  S +SLPLST GRWIIDS++G RVKLVCVNWPSHTQSMLIE
Sbjct: 29  MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 88

Query: 61  GLNHRPLKELADEVVKLRFNCVRLTYATHMFTRYANRTVAENFDLLDLEQAKAGLAQYNP 120
           GLNHRPLKELADE +KLRFNCVRLTYATHMFTRYANRTV ENFDLLDLEQAKAGLAQYNP
Sbjct: 89  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 148

Query: 121 FVLNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180
           FVLNKTIAEAYEAVVDVLGASGLMV+ADNHMSQPRWCCSLDDGNGFFG+RYFDPQEWLQG
Sbjct: 149 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 208

Query: 181 LSLVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNY 240
           LSLVAQRF N  TVV MSLRNE+RG MENA DWN YVTQGVTTIHKINP VLV+VSGLNY
Sbjct: 209 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 268

Query: 241 DNDLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGF 300
           DNDLRCLK+KPL V+TLDNKL FEVHLYSFSGDSE+KFVQQPLNNICA I++ F DHA F
Sbjct: 269 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 328

Query: 301 VMEGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNA 360
           V+EGP+PFPLFVSEYGYDQREVD+AENRFMSCF AHLAQKDLDWALWTWQGSYYYREG A
Sbjct: 329 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 388

Query: 361 EPAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVSNDN 420
           E AETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDP SNASFSYVIYH QSGQC+ VSNDN
Sbjct: 389 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 448

Query: 421 KEIFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANGEGLETSLSTDCSGKQSVWTAISNT 480
           KEIFL NCS SSRWSHDNDSTPIKMSSTGLCLKA+GEGLE SLSTDC GKQS+W+AISN+
Sbjct: 449 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 508

Query: 481 KLHLATFTQDGKNLCLQ-IASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELVGTNTI 538
            LHL T T+DGK+LCLQ I SSNSSKIVTNSCICT  +P CLQDTQSQWFELV TNT+
Sbjct: 509 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 566

[Close]

BLAST of Lag0032673 vs. NCBI nr
Match: XP_011658389.1 (glycosyl hydrolase 5 family protein [Cucumis sativus])

HSP 1 Score: 961.8 bits (2485), Expect = 2.4e-276
Identity = 463/538 (86.06%), Postives = 491/538 (91.26%), Query Frame = 0


Query: 1   MGKTFPATLFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIE 60
           M +T    L LALV +FS  S +SLPLST GRWIIDS++G RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEVVKLRFNCVRLTYATHMFTRYANRTVAENFDLLDLEQAKAGLAQYNP 120
           GLNHRPLKELADE +KLRFNCVRLTYATHMFTRYANRTV ENFDLLDLEQAKAGLAQYNP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 120

Query: 121 FVLNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180
           FVLNKTIAEAYEAVVDVLGASGLMV+ADNHMSQPRWCCSLDDGNGFFG+RYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180

Query: 181 LSLVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNY 240
           LSLVAQRF N  TVV MSLRNE+RG MENA DWN YVTQGVTTIHKINP VLV+VSGLNY
Sbjct: 181 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGF 300
           DNDLRCLK+KPL V+TLDNKL FEVHLYSFSGDSE+KFVQQPLNNICA I++ F DHA F
Sbjct: 241 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 300

Query: 301 VMEGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNA 360
           V+EGP+PFPLFVSEYGYDQREVD+AENRFMSCF AHLAQKDLDWALWTWQGSYYYREG A
Sbjct: 301 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 EPAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVSNDN 420
           E AETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDP SNASFSYVIYH QSGQC+ VSNDN
Sbjct: 361 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 420

Query: 421 KEIFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANGEGLETSLSTDCSGKQSVWTAISNT 480
           KEIFL NCS SSRWSHDNDSTPIKMSSTGLCLKA+GEGLE SLSTDC GKQS+W+AISN+
Sbjct: 421 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 480

Query: 481 KLHLATFTQDGKNLCLQ-IASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELVGTNTI 538
            LHL T T+DGK+LCLQ I SSNSSKIVTNSCICT  +P CLQDTQSQWFELV TNT+
Sbjct: 481 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

[Close]

BLAST of Lag0032673 vs. NCBI nr
Match: KAA0042711.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 954.1 bits (2465), Expect = 5.0e-274
Identity = 450/535 (84.11%), Postives = 494/535 (92.34%), Query Frame = 0


Query: 3   KTFPATLFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIEGL 62
           +TF   L +ALVF+FS LST+SLPLSTRGRWIIDS+TG RVKLVC+NWPSHTQSMLIEGL
Sbjct: 2   RTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEVVKLRFNCVRLTYATHMFTRYANRTVAENFDLLDLEQAKAGLAQYNPFV 122
           NHRPLK+LADE +KLRFNCVRLTYATHMFTRYANRT+ ENFDLLDL+QAKAGLAQYNPFV
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 LNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLS 182
           LNKTIAEAYEAVVDVLGASGLMV+ADNH+SQPRWCCSLDDGNGFFG+  FDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNYDN 242
           LVAQRF N  TVV MSLRNEIRG MENA DWNKY+TQGVTTIH IN +VLV+VSGLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 243 DLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGFVM 302
           DLRCLKEKPL V TLDNKLVFEVHLYSFSGDSE+KFV+QPLN+ICANI+NGF DHAGFVM
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVM 301

Query: 303 EGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNAEP 362
           +G +PFPLFVSE+GYDQREV++AENRFMSCF AHLA +DLDWALW WQGSYY+REG AEP
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 363 AETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVSNDNKE 422
            E+FGVL+SNWTQ+KNPNF +KFQLLQTMLQDPNSN SFSYVIYHPQS QC+ VSNDNKE
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKE 421

Query: 423 IFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANGEGLETSLSTDCSGKQSVWTAISNTKL 482
           IFL +CS  +RWSH ND+TPIKMSSTGLCLKA+GEGLE SLSTDC  +QSVW+AISN+KL
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKL 481

Query: 483 HLATFTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELVGTNTI 538
           HLAT TQ GK+LCLQI SSNSSK+VTNSCICTNG+PNCLQDT+SQWFELVGTNT+
Sbjct: 482 HLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

[Close]

BLAST of Lag0032673 vs. NCBI nr
Match: XP_023533776.1 (uncharacterized protein LOC111795530 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 952.6 bits (2461), Expect = 1.5e-273
Identity = 458/537 (85.29%), Postives = 485/537 (90.32%), Query Frame = 0


Query: 1   MGKTFPATLFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIE 60
           MG+TF   LFLAL F+F   S +SLPLST+GRWIIDS TG RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGRTFQVVLFLAL-FVFLSPSVYSLPLSTKGRWIIDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEVVKLRFNCVRLTYATHMFTRYANRTVAENFDLLDLEQAKAGLAQYNP 120
           GLNHRPLKELADE +KLRFNCVRLTYATHMFTRYANRTV ENFDLLDLE AKAGLAQ NP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEPAKAGLAQNNP 120

Query: 121 FVLNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180
           FVLNKTIAEAYEAVVDVLG SGLMV+ADNH+SQPRWCCSLDDGNGFFGDRYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180

Query: 181 LSLVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNY 240
           LSLVAQRF    TVV MSLRNEIRG  ENA DWN YVTQGVTTIH INP+VLV+VSGLNY
Sbjct: 181 LSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGF 300
           DNDLRCLKEKPL V+TLDNKLVFEVHLYSFSGDSE+KF+ QPLNNICANIINGF DHA F
Sbjct: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFINQPLNNICANIINGFVDHAEF 300

Query: 301 VMEGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNA 360
           V EG +PFPLFVSEYGYDQREV++AENRFMSCF AHLAQKDLDWALWTWQGSYYYREG A
Sbjct: 301 VTEGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 EPAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVSNDN 420
           EP E FGVL+SNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQC+ VSNDN
Sbjct: 361 EPGEAFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDN 420

Query: 421 KEIFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANGEGLETSLSTDCSGKQSVWTAISNT 480
           K+IFL NCS SSRW+HDNDSTPI+MSSTGLCLK +GEGL  SLSTDC G Q+ W+AISNT
Sbjct: 421 KDIFLGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCLGPQNSWSAISNT 480

Query: 481 KLHLATFTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELVGTNTI 538
           KLHLAT   D K+LCLQI SSNSSKIVTNS ICTNG PNCL+DT+SQWFELV TNT+
Sbjct: 481 KLHLATIAPDEKSLCLQIESSNSSKIVTNSFICTNGAPNCLEDTRSQWFELVKTNTL 536

[Close]

BLAST of Lag0032673 vs. NCBI nr
Match: XP_022995752.1 (uncharacterized protein LOC111491191 [Cucurbita maxima])

HSP 1 Score: 949.1 bits (2452), Expect = 1.6e-272
Identity = 455/537 (84.73%), Postives = 483/537 (89.94%), Query Frame = 0


Query: 1   MGKTFPATLFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIE 60
           MG+ F    FLAL F+F     +SLPLST+ +WIIDS TG RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGRIFQVVFFLAL-FVFLSPYVYSLPLSTKEKWIIDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEVVKLRFNCVRLTYATHMFTRYANRTVAENFDLLDLEQAKAGLAQYNP 120
           GLNHRPLKELADE +KLRFNCVRLTYAT MFTRYANRTV ENFDLLDLEQAKAGLAQ NP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLAQNNP 120

Query: 121 FVLNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180
           FVLNKTIAEAYEAVVDVLG SGLMV+ADNH+SQPRWCCSLDDGNGFFGDRYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180

Query: 181 LSLVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNY 240
           LSLVAQRF    TVV MSLRNEIRG  ENA DWN YVTQGVTTIH INP+VLV+VSGLNY
Sbjct: 181 LSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGF 300
           DNDLRCLKEKPL V+TLDNKLVFEVHLYSFSGD E+KF+ QPLNNICANIINGF DHA F
Sbjct: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDPESKFINQPLNNICANIINGFVDHAEF 300

Query: 301 VMEGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNA 360
           V EG +PFPLFVSEYGYDQREV++AENRFMSCF AHLAQKDLDWALWTWQGSYYYREG A
Sbjct: 301 VTEGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 EPAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVSNDN 420
           EP ETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQC+ VSNDN
Sbjct: 361 EPGETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDN 420

Query: 421 KEIFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANGEGLETSLSTDCSGKQSVWTAISNT 480
           K+IF+ NCS SSRW+HDNDSTPI+MSS GLCLK  GEGL  SLSTDC G QS W+AISNT
Sbjct: 421 KDIFMGNCSISSRWTHDNDSTPIRMSSMGLCLKTIGEGLTPSLSTDCLGPQSSWSAISNT 480

Query: 481 KLHLATFTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELVGTNTI 538
           KLHLAT +QDGK+LCLQ+ SSNSSKIVTNSCICTNG PNCLQDT+SQWFELV TNT+
Sbjct: 481 KLHLATISQDGKSLCLQVESSNSSKIVTNSCICTNGAPNCLQDTRSQWFELVKTNTL 536

[Close]

BLAST of Lag0032673 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 2.5e-122
Identity = 231/543 (42.54%), Postives = 332/543 (61.14%), Query Frame = 0


Query: 9   LFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIEGLNHRPLK 68
           L  AL+ +     +HSLPL TRGRWI+D  TG RVKL CVNW  H +  L EGLN  P+ 
Sbjct: 13  LLTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVA 72

Query: 69  ELADEVVKLRFNCVRLTYATHMFTR--YANRTVAENFDLLDLEQAKAGLAQYNPFVLNKT 128
            +A  +  L FNCVRLTY+ HM TR  Y N TVA+ F  L+L +A +G+   NP +L+  
Sbjct: 73  TVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLG 132

Query: 129 IAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLVAQ 188
              AY  VV  L  +G+MV+ DNH+S+P+WCC++DDGNGFFGDRYF+P  W++GL L+A 
Sbjct: 133 HVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMAT 192

Query: 189 RFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNYDNDLRC 248
            F N P VVAMSLRNE+RG     I W++++  G  T+HK NP VLV++SGL +D DL  
Sbjct: 193 YFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSF 252

Query: 249 LKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGFVME--G 308
           L   P+T+     K+V+E H YSF     T       N++C N    F+ + GFV     
Sbjct: 253 LPVLPVTL-PFKEKIVYEGHWYSFGVPWRTGLP----NDVCKNETGRFKSNVGFVTSSAN 312

Query: 309 PSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNAEPA- 368
            +  PLF+SE+G DQR V++ +NR+++C +A+LA++DLDWALWT  GSYYYR  + +P  
Sbjct: 313 ATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYR-SDKQPVK 372

Query: 369 ---ETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPN-SNASFSYVIYHPQSGQCVHVSND 428
              ET+G    +W++I+NP+F+ + + +Q  +QDP  +   +  +IYHP SG CV  S  
Sbjct: 373 DFEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVE-SGI 432

Query: 429 NKEIFLNNC-SASSRWSHD-NDSTPIKMSSTGLCLKANGEGLETSLSTDCSG-KQSVWTA 488
              + L +C S  SRW++D +   PI +  +  C+   G GL   ++ +CS    ++W+ 
Sbjct: 433 GNTVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWST 492

Query: 489 ISNTKLHLAT--FTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQ--DTQSQWFELV 536
           +S+ +L L T    +DGK   + +  S S  I TN CIC   + +C    + + QWF+++
Sbjct: 493 VSSAQLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICIT-DSHCYPKLNPEKQWFKVI 547

[Close]

BLAST of Lag0032673 vs. ExPASy Swiss-Prot
Match: P54583 (Endoglucanase E1 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351607 GN=Acel_0614 PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 3.5e-20
Identity = 89/366 (24.32%), Postives = 159/366 (43.44%), Query Frame = 0


Query: 29  TRGRWIIDSKTGNRVKLVCVNW-PSHTQSMLIEGLNHRPLKELADEVVKLRFNCVRLTYA 88
           T GR I+D+     V++  +NW    T + ++ GL  R  + + D++  L +N +RL Y+
Sbjct: 49  TSGREILDA-NNVPVRIAGINWFGFETCNYVVHGLWSRDYRSMLDQIKSLGYNTIRLPYS 108

Query: 89  THMFTRYANRTVAENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVVA 148
             +       T+  + +   + Q   GL      V++K +A A        G  GL ++ 
Sbjct: 109 DDIL---KPGTMPNSINFYQMNQDLQGLTSLQ--VMDKIVAYA--------GQIGLRIIL 168

Query: 149 DNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLVAQRFINNPTVVAMSLRNEIR--- 208
           D H    R  CS    +  +         W+  L  +AQR+  NPTVV   L NE     
Sbjct: 169 DRH----RPDCS--GQSALWYTSSVSEATWISDLQALAQRYKGNPTVVGFDLHNEPHDPA 228

Query: 209 --GAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGL-NYDND-------LRCLKEKPLTV 268
             G  + +IDW     +    +  +NP++L+ V G+ +Y+ D       L+   + P+ +
Sbjct: 229 CWGCGDPSIDWRLAAERAGNAVLSVNPNLLIFVEGVQSYNGDSYWWGGNLQGAGQYPVVL 288

Query: 269 NTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGFVMEGPSPFPLFVSE 328
           N + N+LV+  H Y+ S   +T F      N    I N    + G++    +  P+++ E
Sbjct: 289 N-VPNRLVYSAHDYATSVYPQTWFSDPTFPNNMPGIWN---KNWGYLF-NQNIAPVWLGE 348

Query: 329 YGYD-QREVDEAENRFMSCFMAHLAQKDLD---WALWTWQGSYYYREGNAEPAETFGVLE 377
           +G   Q   D+   + +  ++   AQ   D   W  W+W         N +  +T G+L+
Sbjct: 349 FGTTLQSTTDQTWLKTLVQYLRPTAQYGADSFQWTFWSW---------NPDSGDTGGILK 380

[Close]

BLAST of Lag0032673 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 8.1e-17
Identity = 98/384 (25.52%), Postives = 158/384 (41.15%), Query Frame = 0


Query: 32  RWIIDSKTGNRVKLVCVN-WPSHTQSMLIEGLNHRPLKELADEVVKLRFNCVRLTYATHM 91
           R I+D  +G  V+L  VN +   T + ++ GL  R  K++  ++  L FN VRL +    
Sbjct: 32  RQIVDD-SGKVVQLKGVNVFGFETGNHVMHGLWARNWKDMIVQMQGLGFNAVRLPFCPAT 91

Query: 92  F---TRYANRTVAENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVVA 151
               T  A+   + N DL  L   +         +L+K IAE          A G+ V+ 
Sbjct: 92  LRSDTMPASIDYSRNADLQGLTSLQ---------ILDKVIAE--------FNARGMYVLL 151

Query: 152 DNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLVAQRFINNPTVVAMSLRNEIRGAM 211
           D+H      C  + +   ++   Y + Q WL  L  VA R+ N P V+ + L+NE  GA 
Sbjct: 152 DHHTPD---CAGISE--LWYTGSYTEAQ-WLADLRFVANRYKNVPYVLGLDLKNEPHGAA 211

Query: 212 -----ENAIDWNKYVTQGVTTIHKINPDVLVVVSGLN------------YDNDLRCLKEK 271
                  A DWNK   +G   +  + P  L+ V G+             +  +L+ L   
Sbjct: 212 TWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEGITDNPVCSTNGGIFWGGNLQPLACT 271

Query: 272 PLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLN--NICANIINGFEDHAGFVMEGPSPF 331
           PL +    N+L+   H+Y         FVQ   N  N   N+   +E H G   +     
Sbjct: 272 PLNIPA--NRLLLAPHVY-----GPDVFVQSYFNDSNFPNNMPAIWERHFG---QFAGTH 331

Query: 332 PLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWAL-WTWQGSYYYREGNAEPAETFG 391
            L + E+G    E D  +  +    + +L  K ++    W+W         N    +T G
Sbjct: 332 ALLLGEFGGKYGEGDARDKTWQDALVKYLRSKGINQGFYWSW---------NPNSGDTGG 368

[Close]

BLAST of Lag0032673 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 2.4e-16
Identity = 75/372 (20.16%), Postives = 156/372 (41.94%), Query Frame = 0


Query: 29  TRGRWIIDSKTGNRVKLVCVNW-PSHTQSMLIEGLNHRPLKELADEVVKLRFNCVRLTYA 88
           T+G  I+D ++G       +NW    T +  + GL  R + ++ D+V K  +N +RL Y+
Sbjct: 41  TQGNKIVD-ESGKEAAFNGLNWFGLETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYS 100

Query: 89  THMFTRYANRTVAENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVVA 148
             +F        +   D +D  +        NP ++     +  + +++  G  G+ ++ 
Sbjct: 101 NQLFDS------SSRPDSIDYHK--------NPDLVGLNPIQIMDKLIEKAGQRGIQIIL 160

Query: 149 DNHMSQPRWCCSLDDGNGFFGDRYFDPQ----EWLQGLSLVAQRFINNPTVVAMSLRNEI 208
           D H            G+G   + ++  Q     W+    ++A R+ NNPTV+   L NE 
Sbjct: 161 DRHR----------PGSGGQSELWYTSQYPESRWISDWKMLADRYKNNPTVIGADLHNEP 220

Query: 209 RGAM-----ENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNYD-----------NDLRCL 268
            G         + DW     +    I  +NP+ L++V G++++            +L  +
Sbjct: 221 HGQASWGTGNASTDWRLAAQRAGNAILSVNPNWLILVEGVDHNVQGNNSQYWWGGNLTGV 280

Query: 269 KEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGFVMEGPSP 328
              P+ ++ + N++V+  H Y   G S   +   P     +N+   ++   G++ +  + 
Sbjct: 281 ANYPVVLD-VPNRVVYSPHDYG-PGVSSQPWFNDPA--FPSNLPAIWDQTWGYISK-QNI 340

Query: 329 FPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNAEPAETFG 380
            P+ V E+G    ++   E ++ +  + ++   +L +  W+          N    +T G
Sbjct: 341 APVLVGEFGGRNVDLSCPEGKWQNALVHYIGANNLYFTYWSL---------NPNSGDTGG 373

[Close]

BLAST of Lag0032673 vs. ExPASy Swiss-Prot
Match: P10474 (Endoglucanase/exoglucanase B OS=Caldicellulosiruptor saccharolyticus OX=44001 GN=celB PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 9.3e-13
Identity = 83/399 (20.80%), Postives = 160/399 (40.10%), Query Frame = 0


Query: 22  THSLPLSTRGRWI------IDSKTGNRVKLVCVNWPSH-TQSMLIEGLNHRPLKELADEV 81
           T S+P  T   W+      I  K G  V L  +NW  + T + + +G+    LK+   E+
Sbjct: 618 TSSIPDDTNDDWLYVSGNKIVDKDGRPVWLTGINWFGYNTGTNVFDGVWSCNLKDTLAEI 677

Query: 82  VKLRFNCVRLTYATHMFTRYANRTVAENFDLLDLEQAKAGLAQY-NPFVLNKTIAEAYEA 141
               FN +R+  +  +   ++                K  +  Y NP +  K   E ++ 
Sbjct: 678 ANRGFNLLRVPISAELILNWSQGI-----------YPKPNINYYVNPELEGKNSLEVFDI 737

Query: 142 VVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLVAQRFINNPT 201
           VV      GL ++ D H  +     ++      + D  F P+++ +    +  R+ N+ T
Sbjct: 738 VVQTCKEVGLKIMLDIHSIKTD---AMGHIYPVWYDEKFTPEDFYKACEWITNRYKNDDT 797

Query: 202 VVAMSLRNEIRG---------AMENAIDWN--KYVTQG-VTTIHKINPDVLVVVSGLN-- 261
           ++A  L+NE  G           +N+ D N  KY  +     I  INP++L+V+ G+   
Sbjct: 798 IIAFDLKNEPHGKPWQDTTFAKWDNSTDINNWKYAAETCAKRILNINPNLLIVIEGIEAY 857

Query: 262 -------------------YDNDLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSE----T 321
                              +  +LR +++ P+ +    NK+V+  H Y  S   +     
Sbjct: 858 PKDDVTWTSKSSSDYYSTWWGGNLRGVRKYPINLGKYQNKVVYSPHDYGPSVYQQPWFYP 917

Query: 322 KFVQQPLNNICANIINGFEDHAGFVMEGPSPFPLFVSEYGYDQREVDEAEN-RFMSCFMA 375
            F ++ L   C      +  +  ++ME  +  PL + E+G     +D A+N ++M     
Sbjct: 918 GFTKESLLQDC------WRPNWAYIME-ENIAPLLIGEWG---GHLDGADNEKWMKYLRD 977

[Close]

BLAST of Lag0032673 vs. ExPASy TrEMBL
Match: A0A0A0K853 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 1.2e-276
Identity = 463/538 (86.06%), Postives = 491/538 (91.26%), Query Frame = 0


Query: 1   MGKTFPATLFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIE 60
           M +T    L LALV +FS  S +SLPLST GRWIIDS++G RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEVVKLRFNCVRLTYATHMFTRYANRTVAENFDLLDLEQAKAGLAQYNP 120
           GLNHRPLKELADE +KLRFNCVRLTYATHMFTRYANRTV ENFDLLDLEQAKAGLAQYNP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 120

Query: 121 FVLNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180
           FVLNKTIAEAYEAVVDVLGASGLMV+ADNHMSQPRWCCSLDDGNGFFG+RYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180

Query: 181 LSLVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNY 240
           LSLVAQRF N  TVV MSLRNE+RG MENA DWN YVTQGVTTIHKINP VLV+VSGLNY
Sbjct: 181 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGF 300
           DNDLRCLK+KPL V+TLDNKL FEVHLYSFSGDSE+KFVQQPLNNICA I++ F DHA F
Sbjct: 241 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 300

Query: 301 VMEGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNA 360
           V+EGP+PFPLFVSEYGYDQREVD+AENRFMSCF AHLAQKDLDWALWTWQGSYYYREG A
Sbjct: 301 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 EPAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVSNDN 420
           E AETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDP SNASFSYVIYH QSGQC+ VSNDN
Sbjct: 361 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 420

Query: 421 KEIFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANGEGLETSLSTDCSGKQSVWTAISNT 480
           KEIFL NCS SSRWSHDNDSTPIKMSSTGLCLKA+GEGLE SLSTDC GKQS+W+AISN+
Sbjct: 421 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 480

Query: 481 KLHLATFTQDGKNLCLQ-IASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELVGTNTI 538
            LHL T T+DGK+LCLQ I SSNSSKIVTNSCICT  +P CLQDTQSQWFELV TNT+
Sbjct: 481 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

[Close]

BLAST of Lag0032673 vs. ExPASy TrEMBL
Match: A0A5A7TMC9 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002090 PE=3 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.4e-274
Identity = 450/535 (84.11%), Postives = 494/535 (92.34%), Query Frame = 0


Query: 3   KTFPATLFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIEGL 62
           +TF   L +ALVF+FS LST+SLPLSTRGRWIIDS+TG RVKLVC+NWPSHTQSMLIEGL
Sbjct: 2   RTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEVVKLRFNCVRLTYATHMFTRYANRTVAENFDLLDLEQAKAGLAQYNPFV 122
           NHRPLK+LADE +KLRFNCVRLTYATHMFTRYANRT+ ENFDLLDL+QAKAGLAQYNPFV
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 LNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLS 182
           LNKTIAEAYEAVVDVLGASGLMV+ADNH+SQPRWCCSLDDGNGFFG+  FDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNYDN 242
           LVAQRF N  TVV MSLRNEIRG MENA DWNKY+TQGVTTIH IN +VLV+VSGLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 243 DLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGFVM 302
           DLRCLKEKPL V TLDNKLVFEVHLYSFSGDSE+KFV+QPLN+ICANI+NGF DHAGFVM
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVM 301

Query: 303 EGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNAEP 362
           +G +PFPLFVSE+GYDQREV++AENRFMSCF AHLA +DLDWALW WQGSYY+REG AEP
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 363 AETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVSNDNKE 422
            E+FGVL+SNWTQ+KNPNF +KFQLLQTMLQDPNSN SFSYVIYHPQS QC+ VSNDNKE
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKE 421

Query: 423 IFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANGEGLETSLSTDCSGKQSVWTAISNTKL 482
           IFL +CS  +RWSH ND+TPIKMSSTGLCLKA+GEGLE SLSTDC  +QSVW+AISN+KL
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKL 481

Query: 483 HLATFTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELVGTNTI 538
           HLAT TQ GK+LCLQI SSNSSK+VTNSCICTNG+PNCLQDT+SQWFELVGTNT+
Sbjct: 482 HLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

[Close]

BLAST of Lag0032673 vs. ExPASy TrEMBL
Match: A0A6J1JZU8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=3 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 7.8e-273
Identity = 455/537 (84.73%), Postives = 483/537 (89.94%), Query Frame = 0


Query: 1   MGKTFPATLFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIE 60
           MG+ F    FLAL F+F     +SLPLST+ +WIIDS TG RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGRIFQVVFFLAL-FVFLSPYVYSLPLSTKEKWIIDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEVVKLRFNCVRLTYATHMFTRYANRTVAENFDLLDLEQAKAGLAQYNP 120
           GLNHRPLKELADE +KLRFNCVRLTYAT MFTRYANRTV ENFDLLDLEQAKAGLAQ NP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLAQNNP 120

Query: 121 FVLNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180
           FVLNKTIAEAYEAVVDVLG SGLMV+ADNH+SQPRWCCSLDDGNGFFGDRYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180

Query: 181 LSLVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNY 240
           LSLVAQRF    TVV MSLRNEIRG  ENA DWN YVTQGVTTIH INP+VLV+VSGLNY
Sbjct: 181 LSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLNY 240

Query: 241 DNDLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGF 300
           DNDLRCLKEKPL V+TLDNKLVFEVHLYSFSGD E+KF+ QPLNNICANIINGF DHA F
Sbjct: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDPESKFINQPLNNICANIINGFVDHAEF 300

Query: 301 VMEGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNA 360
           V EG +PFPLFVSEYGYDQREV++AENRFMSCF AHLAQKDLDWALWTWQGSYYYREG A
Sbjct: 301 VTEGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 361 EPAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVSNDN 420
           EP ETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQC+ VSNDN
Sbjct: 361 EPGETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDN 420

Query: 421 KEIFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANGEGLETSLSTDCSGKQSVWTAISNT 480
           K+IF+ NCS SSRW+HDNDSTPI+MSS GLCLK  GEGL  SLSTDC G QS W+AISNT
Sbjct: 421 KDIFMGNCSISSRWTHDNDSTPIRMSSMGLCLKTIGEGLTPSLSTDCLGPQSSWSAISNT 480

Query: 481 KLHLATFTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELVGTNTI 538
           KLHLAT +QDGK+LCLQ+ SSNSSKIVTNSCICTNG PNCLQDT+SQWFELV TNT+
Sbjct: 481 KLHLATISQDGKSLCLQVESSNSSKIVTNSCICTNGAPNCLQDTRSQWFELVKTNTL 536

[Close]

BLAST of Lag0032673 vs. ExPASy TrEMBL
Match: A0A5A7THG1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002110 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 3.0e-272
Identity = 447/535 (83.55%), Postives = 491/535 (91.78%), Query Frame = 0


Query: 3   KTFPATLFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIEGL 62
           +TF   L LALVF+FS LST+SLPLSTRGRWIID +TG RVKLVC+NWPSHTQSMLIEGL
Sbjct: 2   RTFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEVVKLRFNCVRLTYATHMFTRYANRTVAENFDLLDLEQAKAGLAQYNPFV 122
           NHRPLK+LADE +KLRFNCVRLTYATHMFTRYANRT+ ENFDLLDL+QAKAGLAQYNPFV
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 LNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLS 182
           LNKTIAEAYEAVVD+LGASGLMV+ADNH+SQPRWCCSLDDGNGFFG+  FDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNYDN 242
           LVAQRF N  TVV MSLRNEIRG MENA DWNKY+TQGVTTIH IN +VLV+VSGLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 243 DLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGFVM 302
           DLRCLKEKPL V TLDNKLVFEVHLYSFSGDSE+KFV+QPLNNICANI+NGF DHAGFVM
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVM 301

Query: 303 EGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNAEP 362
           +G +PFPLFVSE+GYDQREV++AENRFMSCF AHLA +DLDWALW WQGSYY+REG AEP
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 363 AETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVSNDNKE 422
            E+FGVL+SNWTQ+KNPNF +KFQLLQTMLQDPNSNASFSYVIYHPQ+ QC+ VSNDNKE
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNKE 421

Query: 423 IFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANGEGLETSLSTDCSGKQSVWTAISNTKL 482
           IFL +CS  +RWSH ND+TPIKMSSTGLCLKA+GEGLE SLSTDC  +QSVW+AISN+KL
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSKL 481

Query: 483 HLATFTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELVGTNTI 538
           HLAT  Q G +LCLQI SSNSSK+VTNSCICTNG+PNCLQDT+SQWFELV TNT+
Sbjct: 482 HLATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

[Close]

BLAST of Lag0032673 vs. ExPASy TrEMBL
Match: A0A6J1F4E4 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111440529 PE=3 SV=1)

HSP 1 Score: 945.3 bits (2442), Expect = 1.1e-271
Identity = 447/511 (87.48%), Postives = 472/511 (92.37%), Query Frame = 0


Query: 27  LSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEVVKLRFNCVRLTY 86
           L +RGRWI+DS TG RVKLVCVNWPSHTQSMLIEGLNHRPLKELADE +KLRFNCVRLTY
Sbjct: 24  LKSRGRWIVDSTTGLRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCVRLTY 83

Query: 87  ATHMFTRYANRTVAENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVV 146
           AT MFTRYANRTV ENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLG SGLMV+
Sbjct: 84  ATQMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGESGLMVI 143

Query: 147 ADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLVAQRFINNPTVVAMSLRNEIRGA 206
           ADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLVAQRF    TVV MSLRNEIRG 
Sbjct: 144 ADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLVAQRFSKKSTVVGMSLRNEIRGT 203

Query: 207 MENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNYDNDLRCLKEKPLTVNTLDNKLVFEVH 266
            ENA DWN YVTQGVTTIH INP+VLV+VSGLN+DNDLRCLKEKPL V+TLDNKLVFEVH
Sbjct: 204 NENANDWNNYVTQGVTTIHNINPNVLVIVSGLNFDNDLRCLKEKPLNVSTLDNKLVFEVH 263

Query: 267 LYSFSGDSETKFVQQPLNNICANIINGFEDHAGFVMEGPSPFPLFVSEYGYDQREVDEAE 326
           LYSFSGD E+KF+ QPLNNICANIINGF DHA FV EGP+PFPLFVSEYGYDQREV++AE
Sbjct: 264 LYSFSGDQESKFINQPLNNICANIINGFVDHAEFVREGPNPFPLFVSEYGYDQREVNDAE 323

Query: 327 NRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNAEPAETFGVLESNWTQIKNPNFVQKFQ 386
           NRFMSCF AHLAQ+DLDWALWTWQGSYYYREG A PAETFGVL+SNWTQIKNPNFVQKFQ
Sbjct: 324 NRFMSCFTAHLAQEDLDWALWTWQGSYYYREGQAGPAETFGVLDSNWTQIKNPNFVQKFQ 383

Query: 387 LLQTMLQDPNSNASFSYVIYHPQSGQCVHVSNDNKEIFLNNCSASSRWSHDNDSTPIKMS 446
           LLQTMLQDPNSNASFSYVIYHPQSGQC+ VSNDNK+IF+ NCS SSRW+HDNDSTPI+MS
Sbjct: 384 LLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKDIFMGNCSISSRWTHDNDSTPIRMS 443

Query: 447 STGLCLKANGEGLETSLSTDCSGKQSVWTAISNTKLHLATFTQDGKNLCLQIASSNSSKI 506
           STGLCLK +GEGL  SLSTDC G QS WTAISNTKLHLAT TQDGK+LCLQ+ SSNSSKI
Sbjct: 444 STGLCLKTSGEGLMPSLSTDCFGPQSSWTAISNTKLHLATVTQDGKSLCLQVESSNSSKI 503

Query: 507 VTNSCICTNGEPNCLQDTQSQWFELVGTNTI 538
           VTNSCICT+G PNCLQDTQSQWFELV TNT+
Sbjct: 504 VTNSCICTDGAPNCLQDTQSQWFELVETNTL 534

[Close]

BLAST of Lag0032673 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 411.4 bits (1056), Expect = 1.1e-114
Identity = 212/534 (39.70%), Postives = 322/534 (60.30%), Query Frame = 0


Query: 9   LFLALVFMFSFLSTH-----SLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIEGLN 68
           +F    F FSF++ +     S PLST  RWI+D + G RVKLVC NWPSH Q ++ EGL+
Sbjct: 12  IFPFFCFFFSFIAQNTVPNMSYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLS 71

Query: 69  HRPLKELADEVVKLRFNCVRLTYATHMFTRYA---NRTVAENFDLLDLEQAKAGLAQYNP 128
            +P+  +A ++V++ FNCVRLT+   + T      N TV ++F  L L     G    NP
Sbjct: 72  KQPVDAVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNP 131

Query: 129 FVLNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQG 188
            +++  + EAY+ VV  LG + +MV+ DNH+++P WCC+ DDGNGFFGD++FDP  W+  
Sbjct: 132 SIIDLPLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAA 191

Query: 189 LSLVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNY 248
           L  +A  F     VV MSLRNE+RG  +N  DW KY+ QG   +H  N  VLV++SGL++
Sbjct: 192 LKKMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSF 251

Query: 249 DNDLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGF 308
           D DL  ++ +P+ + +   KLVFE+H YSFS D  +     P N+IC  ++N   +  G+
Sbjct: 252 DADLSFVRSRPVKL-SFTGKLVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGGY 311

Query: 309 VMEGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNA 368
           ++     FPLF+SE+G D+R V+  +NR+  C     A+ D+DW+LW   GSYY R+G  
Sbjct: 312 LLN--QGFPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKV 371

Query: 369 EPAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVS-ND 428
              E +GVL+S+W  ++N +F+QK   LQ+ LQ P        +++HP +G C+  S +D
Sbjct: 372 GMNEYYGVLDSDWISVRNSSFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDD 431

Query: 429 NKEIFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANG-EGLETSLSTDCSGKQSVWTAIS 488
            K + L  C++S  WS+   +  IK     LCL++NG +   T   T CS   S W  IS
Sbjct: 432 PKMLTLGPCNSSEPWSYTKKALRIK--DQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTIS 491

Query: 489 NTKLHLATFTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELV 533
            +++HLA+ T +  +LCL + ++N+  +V N+C C + + +C  +  SQWF+++
Sbjct: 492 ASRMHLASTTSNKTSLCLDVDTANN--VVANACKCLSKDKSC--EPMSQWFKII 533

[Close]

BLAST of Lag0032673 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 392.1 bits (1006), Expect = 7.1e-109
Identity = 215/535 (40.19%), Postives = 314/535 (58.69%), Query Frame = 0


Query: 10  FLALVFMFSF-LSTHSLPLSTRGRWII-DSKTGNRVKLVCVNWPSHTQSMLIEGLNHRPL 69
           F   VF+  + ++T + P ST  RWI+ D   G RVKL CVNWPSH ++ + EGL+ +PL
Sbjct: 5   FFISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPL 64

Query: 70  KELADEVVKLRFNCVRLTYATHMFTR---YANRTVAENFDLLDLEQAKAGLAQYNPFVLN 129
             +A+++V + FNCVRLT+  ++ T     A  TV ++     L +A +G   +NP +L+
Sbjct: 65  DAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILD 124

Query: 130 KTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLV 189
             + +A++ VV  L    +MV+ DNH+SQP WCCS +DGNGFFGD++ +PQ W++GL  +
Sbjct: 125 LPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKM 184

Query: 190 AQRFIN-NPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNYDND 249
           A  F N +  VV MSLRNE+RG  +N  DW KY+ +G   +H +NP+VLV+VSGLNY  D
Sbjct: 185 ASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATD 244

Query: 250 LRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGFVME 309
           L  L+E+P  V +   K+VFE+H Y F    E       LN IC          +GF++E
Sbjct: 245 LSFLRERPFEV-SFRRKVVFEIHWYGFWNTWE----GDNLNKICGKETEKMMKMSGFLLE 304

Query: 310 GPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNAEPA 369
                PLFVSE+G DQR  +  +N+F+SCFMA  A +DLDW+LWT  GSYY RE +    
Sbjct: 305 --KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSD 364

Query: 370 ETFGVLESNWTQIKNPNFVQKFQLLQT-----MLQDPNSNASFSYVIYHPQSGQCVHVSN 429
           E++GVL+ NW+ I+N   +Q    +QT     M   P        +++HP +G C+ V  
Sbjct: 365 ESYGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK------IMFHPSTGLCI-VRK 424

Query: 430 DNKEIFLNNCSASSRWSHDNDSTPIKMSSTGLCLKANGEGLETSLSTDCSGKQ-SVWTAI 489
              ++ L +C+ S  W   +           LCLKA  +G    L    S    S W   
Sbjct: 425 SLFQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLF 484

Query: 490 SNTKLHLATFTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELV 533
           S++K+ L++ T++G ++CL + + N++ IVTNSC C  G  +C  D +SQWF+LV
Sbjct: 485 SDSKMQLSSITKNGFSVCLDVDTENNN-IVTNSCKCLRGNSSC--DPRSQWFKLV 522

[Close]

BLAST of Lag0032673 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 389.8 bits (1000), Expect = 3.5e-108
Identity = 202/513 (39.38%), Postives = 309/513 (60.23%), Query Frame = 0


Query: 26  PLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEVVKLRFNCVRLT 85
           PLST  RWIID K G RVKL CVNWPSH Q ++ EGL+ + + +LA +++ + FNCVR T
Sbjct: 4   PLSTNSRWIIDEK-GQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 86  YATHMFTRYA---NRTVAENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASG 145
           +   + T      N TV ++F  L L    +G    NP +++  + EAY+ VV  LG + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 146 LMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLVAQRFINNPTVVAMSLRNE 205
           +MV+ DNH+++P WCC  +DGNGFFGD +FDP  W+ GL+ +A  F     VV MSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 206 IRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNYDNDLRCLKEKPLTVNTLDNKLV 265
           +RG  +N  DW KY+ QG   +H+ NP+VLV++SGL+YD DL  ++ + + + T   KLV
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNL-TFTRKLV 243

Query: 266 FEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGFVMEGPSPFPLFVSEYGYDQREV 325
           FE+H YSF+ ++ T   + P N  C  I+   E+  GF +     FP+F+SE+G D R  
Sbjct: 244 FELHRYSFT-NTNTWSSKNP-NEACGEILKSIENGGGFNLR---DFPVFLSEFGIDLRGK 303

Query: 326 DEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNAEPAETFGVLESNWTQIKNPNFV 385
           +  +NR++ C +   A+ D+DW++WT QGSYY REG    +E +G+L+S+W ++++ +F+
Sbjct: 304 NVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFL 363

Query: 386 QKFQLLQTMLQDPNSNASFSYVIYHPQSGQCVHVS-NDNKEIFLNNCSASSRWSHDNDST 445
           Q+  L+ + LQ P S +    +++HP +G C+  S  D  ++ L  C+ S  WS+   +T
Sbjct: 364 QRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQNT 423

Query: 446 PIKMSSTGLCLKANGEGLETSLS-TDCSGKQ-SVWTAISNTKLHLATFTQDGKNLCLQIA 505
            + +    LCL++ G      LS T CS    S W  IS + + LA       +LCL + 
Sbjct: 424 -LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAA-KSTNNSLCLDVD 483

Query: 506 SSNSSKIVTNSCICTNGEPNCLQDTQSQWFELV 533
            +N+  ++ ++C C  GE +   D  SQWF++V
Sbjct: 484 ETNN--LMASNCKCVKGEDSSC-DPISQWFKIV 504

[Close]

BLAST of Lag0032673 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 370.5 bits (950), Expect = 2.2e-102
Identity = 200/530 (37.74%), Postives = 304/530 (57.36%), Query Frame = 0


Query: 14  VFMFSFLSTHSL------PLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIEGLNHRPL 73
           VF+F FLS  SL      PL T+ RWI+++K G+RVKL C NWPSH + ++ EGL+ +P+
Sbjct: 9   VFLFLFLSLISLTLATDYPLFTKSRWIVNNK-GHRVKLACANWPSHLKPVVAEGLSSQPM 68

Query: 74  KELADEVVKLRFNCVRLTYATHMF---TRYANRTVAENFDLLDLEQAKAGLAQYNPFVLN 133
             ++ ++  + FNCVRLT+   +    T   N TV ++F+   L+    G+  +NP+++N
Sbjct: 69  DSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIVN 128

Query: 134 KTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEWLQGLSLV 193
             +   ++AVV  LG   +MV+ DNH + P WCCS DD + FFGD  F+P  W+ GL  +
Sbjct: 129 TPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKKM 188

Query: 194 AQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSGLNYDNDL 253
           A  F+N   VV MSLRNE+RG    + DW KY+ +G   +H  NP+VLV++SGLN+D DL
Sbjct: 189 ATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDADL 248

Query: 254 RCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDHAGFVMEG 313
             LK++P+ + +   KLV E+H YSF+ D   ++    +N+ C+ + +      GFV++ 
Sbjct: 249 SFLKDRPVNL-SFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERRTGGFVLD- 308

Query: 314 PSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYREGNAEPAE 373
              FPLF+SE+G DQR  D   NR+M+C +A  A+KDLDWA+W   G YY+REG     E
Sbjct: 309 -QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVVE 368

Query: 374 TFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQC-VHVSN-DNKE 433
            +G+L++NW  + N  ++++  ++Q     P    +    I+HP +G C V  S+    E
Sbjct: 369 AYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSHCHESE 428

Query: 434 IFLNNCSASSRWSHDNDSTPIKMSSTGLCLKA-NGEGLETSLSTDCSGKQSVWTAISNTK 493
           + L  C+    WS+ +            CL+     G    L   C+  +     IS TK
Sbjct: 429 LTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICTKIEQ----ISATK 488

Query: 494 LHLATFTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQDTQSQWFEL 532
           +HL+  T DG  +CL + S N+  +V NSC C  G+  C  +  SQWF++
Sbjct: 489 MHLSFNTSDGSLVCLDVDSDNN--VVANSCNCLTGDTTC--EPASQWFKI 525

[Close]

BLAST of Lag0032673 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 312.4 bits (799), Expect = 7.1e-85
Identity = 189/540 (35.00%), Postives = 292/540 (54.07%), Query Frame = 0


Query: 1   MGKTFPATLFLALVFMFSFLSTHSLPLSTRGRWIIDSKTGNRVKLVCVNWPSHTQSMLIE 60
           M ++F   LF   +   S L+T S PLST+ RWI+D K G RVKL CVNWP+H Q  + E
Sbjct: 1   MARSFYFCLFFLFISSTSKLTT-SYPLSTKSRWIVDEK-GQRVKLACVNWPAHLQPTVAE 60

Query: 61  GLNHRPLKELADEVVKLRFNCVRLTYATHMFTR---YANRTVAENFDLLDLEQAKAGLAQ 120
           GL+ +PL  ++ ++V + FNCVRLT+   + T        TV ++F+ L L +   G+  
Sbjct: 61  GLSKQPLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQT 120

Query: 121 YNPFVLNKTIAEAYEAVVDVLGASGLMVVADNHMSQPRWCCSLDDGNGFFGDRYFDPQEW 180
           +NP +L+  +  A++ VV  LG +G+MV+ DNH++ P WCC  +D + FFG  +FDP  W
Sbjct: 121 HNPKLLHLPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVW 180

Query: 181 LQGLSLVAQRFINNPTVVAMSLRNEIRGAMENAIDWNKYVTQGVTTIHKINPDVLVVVSG 240
            +GL  +A  F N   V+ MSLRNE RGA +    W +++ QG   +H  NP +LV++SG
Sbjct: 181 AKGLRKMATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSG 240

Query: 241 LNYDNDLRCLKEKPLTVNTLDNKLVFEVHLYSFSGDSETKFVQQPLNNICANIINGFEDH 300
           +++D +L  L+++ + V+  D KLVFE+H YSFS D    + +   N+ C  II     +
Sbjct: 241 IDFDTNLSFLRDRSVNVSFTD-KLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHN 300

Query: 301 AGFVMEGPSPFPLFVSEYGYDQREVDEAENRFMSCFMAHLAQKDLDWALWTWQGSYYYRE 360
            GF++     FPL +SE+G DQR  D + NR+M+C +A  A+ DLDWA+W   G YY R 
Sbjct: 301 GGFLL--GRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRT 360

Query: 361 GNAEPAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCV--H 420
           G                                    PN N     +++HP +G CV  +
Sbjct: 361 GPG--------------------------------LRPNKN-----LLFHPSTGLCVTNN 420

Query: 421 VSNDNKEIFLNNCSASSRWSHDNDSTPIKMSSTGLCLKAN---GEGLETSLSTDCSGKQS 480
            S++   + L  C  S  W+ +     + ++   +C++A    G+ ++  + T CS    
Sbjct: 421 PSDNIPTLRLGPCPKSDPWTFNPSEGILWINK--MCVEAPNVVGQKVKLGVGTKCSKLGQ 480

Query: 481 VWTAISNTKLHLATFTQDGKNLCLQIASSNSSKIVTNSCICTNGEPNCLQDTQSQWFELV 533
               IS TK+HL+  T +G  LCL +   ++S +V N C     + +C  D  SQWF+++
Sbjct: 481 ----ISATKMHLSFKTSNGLLLCLDVDERDNS-VVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:

BLAST of Lag0032673 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8646606.1	2.4e-276	86.06	hypothetical protein Csa_005432 [Cucumis sativus]	[more]
XP_011658389.1	2.4e-276	86.06	glycosyl hydrolase 5 family protein [Cucumis sativus]	[more]
KAA0042711.1	5.0e-274	84.11	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]
XP_023533776.1	1.5e-273	85.29	uncharacterized protein LOC111795530 [Cucurbita pepo subsp. pepo]	[more]
XP_022995752.1	1.6e-272	84.73	uncharacterized protein LOC111491191 [Cucurbita maxima]	[more]

BLAST of Lag0032673 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	2.5e-122	42.54	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P54583	3.5e-20	24.32	Endoglucanase E1 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351...	[more]
P19487	8.1e-17	25.52	Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra...	[more]
P23548	2.4e-16	20.16	Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2	[more]
P10474	9.3e-13	20.80	Endoglucanase/exoglucanase B OS=Caldicellulosiruptor saccharolyticus OX=44001 GN...	[more]

BLAST of Lag0032673 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K853	1.2e-276	86.06	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3...	[more]
A0A5A7TMC9	2.4e-274	84.11	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1JZU8	7.8e-273	84.73	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=...	[more]
A0A5A7THG1	3.0e-272	83.55	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1F4E4	1.1e-271	87.48	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111440529 P...	[more]

BLAST of Lag0032673 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13130.1	1.1e-114	39.70	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26130.1	7.1e-109	40.19	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26140.1	3.5e-108	39.38	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT5G17500.1	2.2e-102	37.74	Glycosyl hydrolase superfamily protein	[more]
AT5G16700.1	7.1e-85	35.00	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 63..350 e-value: 5.1E-26 score: 91.7
None	No IPR available	GENE3D	2.80.10.50		coord: 404..535 e-value: 8.4E-6 score: 28.0
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 24..396 e-value: 7.5E-68 score: 231.3
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 8..533
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 25..386
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 400..512

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0032673.1	Lag0032673.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds