Lag0032649 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032649
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAmino acid transporter family protein
Locationchr11: 35630541 .. 35634145 (-)
RNA-Seq ExpressionLag0032649
SyntenyLag0032649
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTTGTATTTTGTGGTGTTGATACAAGCCGCGGTGAACACCGGAGTGGGAGTAGCCGCAATTTTGCTTGGCGGGGAATGCCTGGAGGTATGTTCTCGGTTGCATATATATCAATGTGTAGGTATATATCTCTCGTAATTGTCAAGATTTTATGCAACAGCCACGTATCGTTGTTCATAGAATAGATCAAACGTTGTTTTCGTTCGGATTAACGATGAGTTTGGTTAGTAATTAATTGGGATTTTGTTGGTGTTGATGATGCATATATGGTACGAGAAAAGTTGATGTATTCAAATGTTTATCCAAAAGGAGAAGTGAAATTGTACCATTTCATAGCAATGGTGACAGTGGGAATGATAATAATGTCTCAGTTTCCGACATTCCATTCCCTCAGATACATCAATTTTCTTTCTCTTCTTCTCAGCCTCGCCTATGCTTTCTTCCTGGTTTTTGCTTCCATTCTTGCAGGTTAATTATTATTAATTTACCCTAAACCTTCTTAATTTTCAATATTAATTACTTATTGTTCTCTATAATTAATAAACTAATTTGTTTTTTAATTAGGTACATCGGATAATGTTCCAGCAAGGGATTATTCTTTGGAATCCACTCTGTCTTCGAGGGTTTTTAGTGCCTTCACTTCCATATCTATCTTCGCTGCCATTTTTGGCAATGGCATTCTTCCTGAAATTCAAGTAATATTTTACCAACTCTTTCTAATTCTATATTTATATATATATTAATGTTTTGGTTTATAGTTTCAAAGCTTTCAATTTGGATTCTTTCTTTGGTTTAGAATTTCAATTTAATTCTTATATATATATGGTTTGGTCCGGACTTCACTATCGTAAAAAAAGCTTAAATACTATTTTGGTCTCAGTACTTTTGACTTTAGTTCATTTTAGTCCTTGTATTTTTAAAATGTTCATTTTGGTTTCTGTACTTTCAAAATGTTCAATTTGATCTTTTATTTTCAATTTTTTTTCTCGTTTTTTCGACCGTAATTTTAACATGACACTTCACTTACTAATTTTTTTGAGATGTAGAGTGATCATATATTATTTGATTAAAGAGTTTATGCTTAGATTTTGCAAATAGAGACTAAATTGGTTACTTTTTAAAAGTACATGGACTAAATGAACATTTTGAAAGTACGGGGACCAAAATAAACCAAAGCTTAAAGTACAGAGACCAAAATGAAAAATTTAAAATACAACAGGATCCAAAATGAACAACCAAAATAGTATTTAAACAAAAAAAAAAAAAGGAAAAAATACATTTTTGGTCCCTGAGGTTTGGGTTAAGTGACACTTTGGTCCCTGAGTTTTCAAACTCAACAGAAATACCCTATAAGGAGCACTTTGGTTCCTGCCGTCAAATTTTCTGTTAACTTTTAACGGTTGCTGACGTGACACTATTATTTTAATATAAAAAATTATATATTTTATTTTTTTAAATAAAAAATATTAAAAAAATAAAATATTAATATTTAATTATTTTTTTTATTCTTTCTCCCTTTCCTTTCTCTCTCTTCTTCTTCTTCTTTTCCATCTTCTCCACCTTCTCCTTCCTCCTTCTTCTTCCTATGTCCGGCGACTGGCGACCGGCGGCCACCGCTCGCCGGCCGCCGGCGACTTTTTTTTTTTTCCTCTTCTTTTCTCTTTTTCTCTTCTTTCTTCACTGATTTTAAAAAAAAAAAAGAAAAAAAGAAAGGGGAGGGAGGAGGGACCTCCCTCCTCCGTCTCCGGCCCTCCTCCCTCCCTCGTTCCCTCCCCTTTCTGTTTTTTTTTTTTTTATTAAAAAAAACAGTGAAGAAAATGTAGGGAAGAAGAAAGAAGAGAAAAAGAGGAAAGAAGAAAAAGGAAAAAAAAATCGCCGACGGCGGCGAGCGGTGGCCGCCGGTCGCCGGACATAGGAAGAAGAAGAAGAGGAAGGAGAAGGTGGAGAAGATGGAGAAGATGGAAAAGAAGAAGAAGAAGATGATGAGAGAGAAAGGGAGAAAGAATAAAAAAATAAAAAATATTAATATTATATATTTTTTAAATATTTTTTATTTAAAAAATAAAATAAATAATTTTTTATATTAAAATAATAGTGCCACGTCAGCAACCGTTAAAAGATAACAGAAAAATTGACGGCAGGGACCAAAGTGCTCCTTATCTCAAACCTCGAGAGTATTTTTGTTGAGTTTGAAAACTTAGGGACCAAAGTGTCACTTAACCCAAACCTCAGGGACCAAAAATGTATTTTTCCCAAAAAAAAAAAAAAAATAGGAGGAAAACAAATACTCACCACAGGGACTTCCCTAAATGTGGAAATTTTAATTATCTCATTGTCCCTTTCTGTGTACTCAAGTAATTAATAGAATTTTACTAGAGTTAGTACATCAAAGTTTAAAGGGTTTATGATGGGAGACAAGTGAAAAATGAAGTCATAAACTCATTTTATAGCACTTGAATATCTGTCATTTTATATTATATTTTTTCATATGTGGGATTTTTGCTCTTGTTCTAATATTTTGCCAAAACTAATACTCACCAATCATTTAAAAGCCATTACTATATGTTTCATAGATTAACTGTCATGTCTAGGATGAGTAAAAAATAAGGAGGACATGATAGATTTCAAGAAGGAAAAAAAAAAAAGGTTAATGAACCATTTGTGAGATTTAAGCGAAGAATAAGATCAAAATTAAAACTTTTAAAATCATAAGAATGAATTTGGAACTAAATTGCAAAGGGACTAAATTAAAACTAAATCCAAACTATAGAGATTTAATTGAAATCAAGTACAAACTATAATAACAAAATTGCTATTTTAACCATTTTAATATATTTTTTTTCTTTTTTCTTTTGTAAATTACCTAAGCCATATATATTACAATGTATACAGGCAACTCTAGCACCACCAGTAGCTGGGAAGATGGTAAAAGGACTCATCATGTGTTACATTGTAATCTTCATCACATTCTACTCAGCTGCAGCTTCTGGCTATTGGGTTTTTGGCAACAAATCCAATTCAAATCTCTTGAAGAACTTATTGCCCCCAAATGAATCTCCTTTAGCTCCCACATGGATGCTATTTCTCGCCGTTCTCTTCATTCTCCTACAACTTCTCGCCATCGGAATGGTTCGAAATACCATCCCTCAGAACCCCATTTGATTACGATTTTGTTTTTAGTTTTACGACTCTTTTAACCTCGTTTCATACGCAGGTTTACGCCCAAGTGGCTTACGAGATAATGGAGAGAAAATCAGCTGATGCACAAAAAGGAGTGTTCTCAAGAAGAAACCTAATTCCTAGGTTGATTTTGAGGACATTGTACATGTCATTGTGTGGTTTCTTTGCAGCTATGTTTCCATTCTTCGGCGACATTAACAGCGTCGTTGGAGCGATCGGGTTCATTCCACTCGACTTCATTCTTCCGATGGTTCTCTACAACATCACCCACAAACCTCCGACGACGTCGATGACTTATTGGGTCAATCTCTTCATTGTTGTTGCCTTCTCAGGTGCAGGAATTATAGGATGCTTTGCTTCTATTAGGAACTTGGTTTTGGATGCCAAGAAGTTTAAGCTCTTTAGTAGTCATGTGGTTTAG

mRNA sequence

ATGGATGTTGTATTTTGTGGTGTTGATACAAGCCGCGGTGAACACCGGAGTGGGAGTAGCCGCAATTTTGCTTGGCGGGGAATGCCTGGAGGAGAAGTGAAATTGTACCATTTCATAGCAATGGTGACAGTGGGAATGATAATAATGTCTCAGTTTCCGACATTCCATTCCCTCAGATACATCAATTTTCTTTCTCTTCTTCTCAGCCTCGCCTATGCTTTCTTCCTGGTTTTTGCTTCCATTCTTGCAGGTACATCGGATAATGTTCCAGCAAGGGATTATTCTTTGGAATCCACTCTGTCTTCGAGGGTTTTTAGTGCCTTCACTTCCATATCTATCTTCGCTGCCATTTTTGGCAATGGCATTCTTCCTGAAATTCAAGCAACTCTAGCACCACCAGTAGCTGGGAAGATGGTAAAAGGACTCATCATGTGTTACATTGTAATCTTCATCACATTCTACTCAGCTGCAGCTTCTGGCTATTGGGTTTTTGGCAACAAATCCAATTCAAATCTCTTGAAGAACTTATTGCCCCCAAATGAATCTCCTTTAGCTCCCACATGGATGCTATTTCTCGCCGTTCTCTTCATTCTCCTACAACTTCTCGCCATCGGAATGGTTTACGCCCAAGTGGCTTACGAGATAATGGAGAGAAAATCAGCTGATGCACAAAAAGGAGTGTTCTCAAGAAGAAACCTAATTCCTAGGTTGATTTTGAGGACATTGTACATGTCATTGTGTGGTTTCTTTGCAGCTATGTTTCCATTCTTCGGCGACATTAACAGCGTCGTTGGAGCGATCGGGTTCATTCCACTCGACTTCATTCTTCCGATGGTTCTCTACAACATCACCCACAAACCTCCGACGACGTCGATGACTTATTGGGTCAATCTCTTCATTGTTGTTGCCTTCTCAGGTGCAGGAATTATAGGATGCTTTGCTTCTATTAGGAACTTGGTTTTGGATGCCAAGAAGTTTAAGCTCTTTAGTAGTCATGTGGTTTAG

Coding sequence (CDS)

ATGGATGTTGTATTTTGTGGTGTTGATACAAGCCGCGGTGAACACCGGAGTGGGAGTAGCCGCAATTTTGCTTGGCGGGGAATGCCTGGAGGAGAAGTGAAATTGTACCATTTCATAGCAATGGTGACAGTGGGAATGATAATAATGTCTCAGTTTCCGACATTCCATTCCCTCAGATACATCAATTTTCTTTCTCTTCTTCTCAGCCTCGCCTATGCTTTCTTCCTGGTTTTTGCTTCCATTCTTGCAGGTACATCGGATAATGTTCCAGCAAGGGATTATTCTTTGGAATCCACTCTGTCTTCGAGGGTTTTTAGTGCCTTCACTTCCATATCTATCTTCGCTGCCATTTTTGGCAATGGCATTCTTCCTGAAATTCAAGCAACTCTAGCACCACCAGTAGCTGGGAAGATGGTAAAAGGACTCATCATGTGTTACATTGTAATCTTCATCACATTCTACTCAGCTGCAGCTTCTGGCTATTGGGTTTTTGGCAACAAATCCAATTCAAATCTCTTGAAGAACTTATTGCCCCCAAATGAATCTCCTTTAGCTCCCACATGGATGCTATTTCTCGCCGTTCTCTTCATTCTCCTACAACTTCTCGCCATCGGAATGGTTTACGCCCAAGTGGCTTACGAGATAATGGAGAGAAAATCAGCTGATGCACAAAAAGGAGTGTTCTCAAGAAGAAACCTAATTCCTAGGTTGATTTTGAGGACATTGTACATGTCATTGTGTGGTTTCTTTGCAGCTATGTTTCCATTCTTCGGCGACATTAACAGCGTCGTTGGAGCGATCGGGTTCATTCCACTCGACTTCATTCTTCCGATGGTTCTCTACAACATCACCCACAAACCTCCGACGACGTCGATGACTTATTGGGTCAATCTCTTCATTGTTGTTGCCTTCTCAGGTGCAGGAATTATAGGATGCTTTGCTTCTATTAGGAACTTGGTTTTGGATGCCAAGAAGTTTAAGCTCTTTAGTAGTCATGTGGTTTAG

Protein sequence

MDVVFCGVDTSRGEHRSGSSRNFAWRGMPGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDNVPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIVIFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVYAQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKLFSSHVV
Homology
BLAST of Lag0032649 vs. NCBI nr
Match: TYK06095.1 (putative GABA transporter 2 [Cucumis melo var. makuwa])

HSP 1 Score: 576.2 bits (1484), Expect = 1.8e-160
Identity = 296/334 (88.62%), Postives = 315/334 (94.31%), Query Frame = 0

Query: 1   MDVVFCGVDTSRGEHRSGSSRNFAWRGMPGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRY 60
           MDVVFCGV TS  +HR GS RN AW GM  GE+KLYHFIA+VTVGMI++SQ P+FHSLRY
Sbjct: 1   MDVVFCGVHTSGRQHRRGSGRNLAWWGMLRGELKLYHFIAVVTVGMIMISQLPSFHSLRY 60

Query: 61  INFLSLLLSLAYAFFLVFASILAGTSDNVPARDYSLESTLSSRVFSAFTSISIFAAIFGN 120
           INFLSLLLSLAYAFF+ FASILAGTSDNVP RDYSLEST S+RVFSAFTSISIFAAIFGN
Sbjct: 61  INFLSLLLSLAYAFFIAFASILAGTSDNVPPRDYSLESTPSARVFSAFTSISIFAAIFGN 120

Query: 121 GILPEIQATLAPPVAGKMVKGLIMCYIVIFITFYSAAASGYWVFGNKSNSNLLKNLLPPN 180
           GILPEIQATLAPP  GKMVKGLIMCYIVIFITFYS+AASGYWVFGNKSNSN+LKNLLP N
Sbjct: 121 GILPEIQATLAPPTGGKMVKGLIMCYIVIFITFYSSAASGYWVFGNKSNSNILKNLLPKN 180

Query: 181 ESPLAPTWMLFLAVLFILLQLLAIGMVYAQVAYEIMERKSADAQKGVFSRRNLIPRLILR 240
           ESPLAPTW+L LAVLFILLQLLAIGMVYAQVAYEIMER+SADA++GVFSRRNLIPRLILR
Sbjct: 181 ESPLAPTWILALAVLFILLQLLAIGMVYAQVAYEIMERRSADAKQGVFSRRNLIPRLILR 240

Query: 241 TLYMSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPTTSMTYWVNLFI 300
           TLYMSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPP TS+TYWVN+FI
Sbjct: 241 TLYMSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPVTSITYWVNVFI 300

Query: 301 VVAFSGAGIIGCFASIRNLVLDAKKFKLFSSHVV 335
           V AFSGAG++GCFASIRNLVLD+KKFKLFSSHVV
Sbjct: 301 VAAFSGAGLLGCFASIRNLVLDSKKFKLFSSHVV 334

BLAST of Lag0032649 vs. NCBI nr
Match: XP_038906705.1 (probable GABA transporter 2 [Benincasa hispida])

HSP 1 Score: 540.4 bits (1391), Expect = 1.1e-149
Identity = 278/306 (90.85%), Postives = 293/306 (95.75%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P GE+KLYHFIAMVT+GMIIMSQ P+FHSLRYINFLSLLLSLAYAFF+ FASILAGTSDN
Sbjct: 144 PKGELKLYHFIAMVTMGMIIMSQLPSFHSLRYINFLSLLLSLAYAFFIAFASILAGTSDN 203

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
           VP RDYSLESTLS+RVFSAFTSISIFAAIFGNGILPEIQATLAPPV GKMVKGLIMCYIV
Sbjct: 204 VPPRDYSLESTLSARVFSAFTSISIFAAIFGNGILPEIQATLAPPVGGKMVKGLIMCYIV 263

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVY 208
           IFITFYS+AASGYWVFGN SNSN+LKNLLP NESPLAPTW+L LAVLFILLQLLAIGMVY
Sbjct: 264 IFITFYSSAASGYWVFGNNSNSNILKNLLPKNESPLAPTWILLLAVLFILLQLLAIGMVY 323

Query: 209 AQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 268
           AQVAYEIMER+SADA++GVFSRRNLIPRLILRTLYMSLCG FAAMFPFFGDINSVVGAIG
Sbjct: 324 AQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLYMSLCGLFAAMFPFFGDINSVVGAIG 383

Query: 269 FIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKL 328
           FIPLDFILPMVLYNITHKPP TS+TYWVNLFIV AFSG G++GCFAS RNLVLD+KKFKL
Sbjct: 384 FIPLDFILPMVLYNITHKPPVTSITYWVNLFIVAAFSGVGLLGCFASFRNLVLDSKKFKL 443

Query: 329 FSSHVV 335
           FSSHVV
Sbjct: 444 FSSHVV 449

BLAST of Lag0032649 vs. NCBI nr
Match: XP_008437380.1 (PREDICTED: probable GABA transporter 2 [Cucumis melo])

HSP 1 Score: 538.5 bits (1386), Expect = 4.1e-149
Identity = 277/306 (90.52%), Postives = 295/306 (96.41%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P GE+KLYHFIA+VTVGMI++SQ P+FHSLRYINFLSLLLSLAYAFF+ FASILAGTSDN
Sbjct: 144 PKGELKLYHFIAVVTVGMIMISQLPSFHSLRYINFLSLLLSLAYAFFIAFASILAGTSDN 203

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
           VP RDYSLEST S+RVFSAFTSISIFAAIFGNGILPEIQATLAPP  GKMVKGLIMCYIV
Sbjct: 204 VPPRDYSLESTPSARVFSAFTSISIFAAIFGNGILPEIQATLAPPTGGKMVKGLIMCYIV 263

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVY 208
           IFITFYS+AASGYWVFGNKSNSN+LKNLLP NESPLAPTW+L LAVLFILLQLLAIGMVY
Sbjct: 264 IFITFYSSAASGYWVFGNKSNSNILKNLLPKNESPLAPTWILALAVLFILLQLLAIGMVY 323

Query: 209 AQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 268
           AQVAYEIMER+SADA++GVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG
Sbjct: 324 AQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 383

Query: 269 FIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKL 328
           FIPLDFILPMVLYNITHKPP TS+TYWVN+FIV AFSGAG++GCFASIRNLVLD+KKFKL
Sbjct: 384 FIPLDFILPMVLYNITHKPPVTSITYWVNVFIVAAFSGAGLLGCFASIRNLVLDSKKFKL 443

Query: 329 FSSHVV 335
           FSSHVV
Sbjct: 444 FSSHVV 449

BLAST of Lag0032649 vs. NCBI nr
Match: KAA0042692.1 (putative GABA transporter 2 [Cucumis melo var. makuwa])

HSP 1 Score: 538.5 bits (1386), Expect = 4.1e-149
Identity = 277/306 (90.52%), Postives = 295/306 (96.41%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P GE+KLYHFIA+VTVGMI++SQ P+FHSLRYINFLSLLLSLAYAFF+ FASILAGTSDN
Sbjct: 145 PKGELKLYHFIAVVTVGMIMISQLPSFHSLRYINFLSLLLSLAYAFFIAFASILAGTSDN 204

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
           VP RDYSLEST S+RVFSAFTSISIFAAIFGNGILPEIQATLAPP  GKMVKGLIMCYIV
Sbjct: 205 VPPRDYSLESTPSARVFSAFTSISIFAAIFGNGILPEIQATLAPPTGGKMVKGLIMCYIV 264

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVY 208
           IFITFYS+AASGYWVFGNKSNSN+LKNLLP NESPLAPTW+L LAVLFILLQLLAIGMVY
Sbjct: 265 IFITFYSSAASGYWVFGNKSNSNILKNLLPKNESPLAPTWILALAVLFILLQLLAIGMVY 324

Query: 209 AQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 268
           AQVAYEIMER+SADA++GVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG
Sbjct: 325 AQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 384

Query: 269 FIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKL 328
           FIPLDFILPMVLYNITHKPP TS+TYWVN+FIV AFSGAG++GCFASIRNLVLD+KKFKL
Sbjct: 385 FIPLDFILPMVLYNITHKPPVTSITYWVNVFIVAAFSGAGLLGCFASIRNLVLDSKKFKL 444

Query: 329 FSSHVV 335
           FSSHVV
Sbjct: 445 FSSHVV 450

BLAST of Lag0032649 vs. NCBI nr
Match: XP_004143875.1 (probable GABA transporter 2 [Cucumis sativus] >KGN50030.1 hypothetical protein Csa_000172 [Cucumis sativus])

HSP 1 Score: 534.3 bits (1375), Expect = 7.7e-148
Identity = 274/306 (89.54%), Postives = 293/306 (95.75%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P GE+KLYHFIA+VT+GMII+SQ P+FHSLRYINFLSLLLSLAYAFF+ FASILAGTSDN
Sbjct: 144 PKGELKLYHFIAVVTLGMIIISQLPSFHSLRYINFLSLLLSLAYAFFIAFASILAGTSDN 203

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
           VP RDYSLEST S+RVFSAFTSISIFAAIFGNGILPEIQATLAPP+ GKMVKGLIMCYIV
Sbjct: 204 VPPRDYSLESTPSARVFSAFTSISIFAAIFGNGILPEIQATLAPPIGGKMVKGLIMCYIV 263

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVY 208
           IFITFYS+AASGYWVFGNKSNSN+LKNLLP NE PLAPTW+L LAVLFILLQLLAIGMVY
Sbjct: 264 IFITFYSSAASGYWVFGNKSNSNILKNLLPKNEPPLAPTWILALAVLFILLQLLAIGMVY 323

Query: 209 AQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 268
           AQVAYEIMER+SADA++GVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG
Sbjct: 324 AQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 383

Query: 269 FIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKL 328
           FIPLDFILPMVLYNITHKPP TS+TYWVN+FIV AFSG G++GCFASIRNLVLD+KKF L
Sbjct: 384 FIPLDFILPMVLYNITHKPPVTSITYWVNVFIVAAFSGVGLLGCFASIRNLVLDSKKFNL 443

Query: 329 FSSHVV 335
           FSSHVV
Sbjct: 444 FSSHVV 449

BLAST of Lag0032649 vs. ExPASy Swiss-Prot
Match: Q8L4X4 (Probable GABA transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g41800 PE=1 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.5e-114
Identity = 213/306 (69.61%), Postives = 252/306 (82.35%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P G +KLY FIAMVTV M+++SQ P+FHSLR+IN  SLLLSL Y F +V A I  G S N
Sbjct: 146 PQGTLKLYEFIAMVTVVMMVLSQLPSFHSLRHINCASLLLSLGYTFLVVGACINLGLSKN 205

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
            P R+YSLE + S +VFSAFTSISI AAIFGNGILPEIQATLAPP  GKM+KGL++CY V
Sbjct: 206 APKREYSLEHSDSGKVFSAFTSISIIAAIFGNGILPEIQATLAPPATGKMLKGLLLCYSV 265

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVY 208
           IF TFYSAA SGYWVFGN S+SN+LKNL+P     LAP  ++ LAV+F+LLQL AIG+VY
Sbjct: 266 IFFTFYSAAISGYWVFGNNSSSNILKNLMPDEGPTLAPIVVIGLAVIFVLLQLFAIGLVY 325

Query: 209 AQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 268
           +QVAYEIME+KSAD  KG+FS+RNL+PRLILRTLYM+ CGF AAM PFFGDIN+VVGA G
Sbjct: 326 SQVAYEIMEKKSADTTKGIFSKRNLVPRLILRTLYMAFCGFMAAMLPFFGDINAVVGAFG 385

Query: 269 FIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKL 328
           FIPLDF+LPM+LYN+T+KP   S TYW+N+ I+V F+ AG++G F+SIR LVLDA KFKL
Sbjct: 386 FIPLDFVLPMLLYNMTYKPTRRSFTYWINMTIMVVFTCAGLMGAFSSIRKLVLDANKFKL 445

Query: 329 FSSHVV 335
           FSS VV
Sbjct: 446 FSSEVV 451

BLAST of Lag0032649 vs. ExPASy Swiss-Prot
Match: F4HW02 (GABA transporter 1 OS=Arabidopsis thaliana OX=3702 GN=GAT1 PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.4e-75
Identity = 142/303 (46.86%), Postives = 204/303 (67.33%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P GE+KL+ F+ +    +++++QFP+FHSLRYIN LSLLL L Y+     ASI  G   N
Sbjct: 147 PNGEMKLFEFVIIFGCLLLVLAQFPSFHSLRYINSLSLLLCLLYSASAAAASIYIGKEPN 206

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
            P +DY++     +RVF  F +++I A  +GNGI+PEIQAT++ PV GKM+KGL MCY+V
Sbjct: 207 APEKDYTIVGDPETRVFGIFNAMAIIATTYGNGIIPEIQATISAPVKGKMMKGLCMCYLV 266

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESP-LAPTWMLFLAVLFILLQLLAIGMV 208
           + +TF++ A +GYW FG K+N  +  N L    +    PTW +FL  LF +LQL A+ +V
Sbjct: 267 VIMTFFTVAITGYWAFGKKANGLIFTNFLNAETNHYFVPTWFIFLVNLFTVLQLSAVAVV 326

Query: 209 YAQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAI 268
           Y Q   +I+E   +D  K  FS RN+IPRL++R+L++ +    AAM PFFGD+NS++GA 
Sbjct: 327 YLQPINDILESVISDPTKKEFSIRNVIPRLVVRSLFVVMATIVAAMLPFFGDVNSLLGAF 386

Query: 269 GFIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFK 328
           GFIPLDF+LP+V +N T KP   S  +W+N  I V FS  G+I   A++R +++DA  +K
Sbjct: 387 GFIPLDFVLPVVFFNFTFKPSKKSFIFWINTVIAVVFSCLGVIAMVAAVRQIIIDANTYK 446

Query: 329 LFS 331
           LF+
Sbjct: 447 LFA 449

BLAST of Lag0032649 vs. ExPASy Swiss-Prot
Match: Q9C733 (Lysine histidine transporter-like 1 OS=Arabidopsis thaliana OX=3702 GN=At1g48640 PE=3 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 5.0e-25
Identity = 80/304 (26.32%), Postives = 156/304 (51.32%), Query Frame = 0

Query: 33  VKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDNVPAR 92
           ++L  FI +      ++S  P F+S+  ++ ++ ++SL+Y+     A+   G  ++V   
Sbjct: 163 IRLSFFIMIFASSHFVLSHLPNFNSISGVSLVAAVMSLSYSTIAWTATAAKGVQEDV--- 222

Query: 93  DYSLES-TLSSRVFSAFTSI-SIFAAIFGNGILPEIQATL----APPVAGKMVKGLIMCY 152
            Y  +S T +S V S FT +  I  A  G+ ++ EIQAT+    + P  G M +G+++ Y
Sbjct: 223 QYGYKSGTTASTVLSFFTGLGGIAFAYAGHNVVLEIQATIPSTPSNPSKGPMWRGVVVAY 282

Query: 153 IVIFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGM 212
           +V+ + ++  A  GY VFGN    N+L +L         P W +  A LF+++ ++    
Sbjct: 283 VVVALCYFPVALVGYGVFGNAVLDNVLMSL-------ETPVWAIATANLFVVMHVIGSYQ 342

Query: 213 VYAQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGA 272
           ++A   ++++E       K +  + + + R I+R +Y++L  F   M PFFG + +  G 
Sbjct: 343 IFAMPVFDMVE---TFLVKKLNFKPSTVLRFIVRNVYVALTMFIGIMIPFFGGLLAFFGG 402

Query: 273 IGFIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKF 331
             F P  + LP +++ + +KP   S+++W N   +V      I+     +R +++ +K +
Sbjct: 403 FAFAPTSYFLPCIMWLLIYKPKRFSLSWWTNWVCIVLGVVLMILSSIGGLRQIIIQSKDY 453

BLAST of Lag0032649 vs. ExPASy Swiss-Prot
Match: Q69LA1 (Probable proline transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=LOC_Os07g01090 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 8.6e-25
Identity = 91/304 (29.93%), Postives = 151/304 (49.67%), Query Frame = 0

Query: 31  GEVKLYHFIAMV-TVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDNV 90
           G +KL + IA+   V  +     P   +LR     S   SL Y       S+  G +   
Sbjct: 141 GVLKLPYCIALSGFVCALFAFGIPYLSALRIWLGFSTFFSLIYITIAFVLSLRDGIT--T 200

Query: 91  PARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIVI 150
           PA+DY++  + S+R+F+   +++     +  G+LPEIQAT+ PPV   M K L   + V 
Sbjct: 201 PAKDYTIPGSHSARIFTTIGAVANLVFAYNTGMLPEIQATIRPPVVKNMEKALWFQFTVG 260

Query: 151 FITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVYA 210
            +  Y+    GYW +G+ ++S LL        S   P W+  +A L   LQ +    ++A
Sbjct: 261 SLPLYAVTFMGYWAYGSSTSSYLL-------NSVKGPVWVKAMANLSAFLQTVIALHIFA 320

Query: 211 QVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIGF 270
              YE ++ K      G F+  N++ R+ +R  Y+++    AAM PF GD  S+ GA+  
Sbjct: 321 SPMYEFLDTKYGSGHGGPFAIHNVMFRVGVRGGYLTVNTLVAAMLPFLGDFMSLTGALST 380

Query: 271 IPLDFILPMVLYNIT--HKPPTTSMT-YWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKF 330
            PL F+L   +Y +   HK  T  ++ +W+N   V  FS   I    A++R +++D++ +
Sbjct: 381 FPLTFVLANHMYLMVKRHKLSTLQISWHWLN---VAGFSLLSIAAAVAALRLIMVDSRTY 432

BLAST of Lag0032649 vs. ExPASy Swiss-Prot
Match: Q9C9J0 (Lysine histidine transporter-like 5 OS=Arabidopsis thaliana OX=3702 GN=At1g71680 PE=2 SV=2)

HSP 1 Score: 114.0 bits (284), Expect = 3.3e-24
Identity = 84/303 (27.72%), Postives = 151/303 (49.83%), Query Frame = 0

Query: 33  VKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDNVPAR 92
           ++  ++I       +++SQ P F+S++ ++ L+ L+S  Y+     ASI  GT ++ P+ 
Sbjct: 157 IRQTYYILGFAALQLVLSQSPDFNSIKIVSLLAALMSFLYSMIASVASIAKGT-EHRPST 216

Query: 93  DYSLESTLSSRVFSAFTSISIFAAIF-GNGILPEIQATL--APPVAGK--MVKGLIMCYI 152
                 T++S VF AF  I   A  F G+ ++ EIQAT+   P V  K  M KG+++ YI
Sbjct: 217 YGVRGDTVASMVFDAFNGIGTIAFAFAGHSVVLEIQATIPSTPEVPSKKPMWKGVVVAYI 276

Query: 153 VIFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMV 212
           ++ I +   A SGYW FG     ++L +L         P W++  A   + + ++    V
Sbjct: 277 IVIICYLFVAISGYWAFGAHVEDDVLISL-------ERPAWLIAAANFMVFIHVIGSYQV 336

Query: 213 YAQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAI 272
           +A + ++ +E       K  F+    + RL+ R+ Y++L    A   PFFG +    G +
Sbjct: 337 FAMIVFDTIESYLVKTLK--FTPSTTL-RLVARSTYVALICLVAVCIPFFGGLLGFFGGL 396

Query: 273 GFIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFK 331
            F    + LP +++ I  +P   S  +W +   +V      I+     +R+++L A+ +K
Sbjct: 397 VFSSTSYFLPCIIWLIMKRPKRFSAHWWCSWVAIVTGISIAILAPIGGMRHIILSARTYK 448

BLAST of Lag0032649 vs. ExPASy TrEMBL
Match: A0A5D3C3Y3 (Putative GABA transporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001960 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 8.6e-161
Identity = 296/334 (88.62%), Postives = 315/334 (94.31%), Query Frame = 0

Query: 1   MDVVFCGVDTSRGEHRSGSSRNFAWRGMPGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRY 60
           MDVVFCGV TS  +HR GS RN AW GM  GE+KLYHFIA+VTVGMI++SQ P+FHSLRY
Sbjct: 1   MDVVFCGVHTSGRQHRRGSGRNLAWWGMLRGELKLYHFIAVVTVGMIMISQLPSFHSLRY 60

Query: 61  INFLSLLLSLAYAFFLVFASILAGTSDNVPARDYSLESTLSSRVFSAFTSISIFAAIFGN 120
           INFLSLLLSLAYAFF+ FASILAGTSDNVP RDYSLEST S+RVFSAFTSISIFAAIFGN
Sbjct: 61  INFLSLLLSLAYAFFIAFASILAGTSDNVPPRDYSLESTPSARVFSAFTSISIFAAIFGN 120

Query: 121 GILPEIQATLAPPVAGKMVKGLIMCYIVIFITFYSAAASGYWVFGNKSNSNLLKNLLPPN 180
           GILPEIQATLAPP  GKMVKGLIMCYIVIFITFYS+AASGYWVFGNKSNSN+LKNLLP N
Sbjct: 121 GILPEIQATLAPPTGGKMVKGLIMCYIVIFITFYSSAASGYWVFGNKSNSNILKNLLPKN 180

Query: 181 ESPLAPTWMLFLAVLFILLQLLAIGMVYAQVAYEIMERKSADAQKGVFSRRNLIPRLILR 240
           ESPLAPTW+L LAVLFILLQLLAIGMVYAQVAYEIMER+SADA++GVFSRRNLIPRLILR
Sbjct: 181 ESPLAPTWILALAVLFILLQLLAIGMVYAQVAYEIMERRSADAKQGVFSRRNLIPRLILR 240

Query: 241 TLYMSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPTTSMTYWVNLFI 300
           TLYMSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPP TS+TYWVN+FI
Sbjct: 241 TLYMSLCGFFAAMFPFFGDINSVVGAIGFIPLDFILPMVLYNITHKPPVTSITYWVNVFI 300

Query: 301 VVAFSGAGIIGCFASIRNLVLDAKKFKLFSSHVV 335
           V AFSGAG++GCFASIRNLVLD+KKFKLFSSHVV
Sbjct: 301 VAAFSGAGLLGCFASIRNLVLDSKKFKLFSSHVV 334

BLAST of Lag0032649 vs. ExPASy TrEMBL
Match: A0A5A7TH10 (Putative GABA transporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G001900 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.0e-149
Identity = 277/306 (90.52%), Postives = 295/306 (96.41%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P GE+KLYHFIA+VTVGMI++SQ P+FHSLRYINFLSLLLSLAYAFF+ FASILAGTSDN
Sbjct: 145 PKGELKLYHFIAVVTVGMIMISQLPSFHSLRYINFLSLLLSLAYAFFIAFASILAGTSDN 204

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
           VP RDYSLEST S+RVFSAFTSISIFAAIFGNGILPEIQATLAPP  GKMVKGLIMCYIV
Sbjct: 205 VPPRDYSLESTPSARVFSAFTSISIFAAIFGNGILPEIQATLAPPTGGKMVKGLIMCYIV 264

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVY 208
           IFITFYS+AASGYWVFGNKSNSN+LKNLLP NESPLAPTW+L LAVLFILLQLLAIGMVY
Sbjct: 265 IFITFYSSAASGYWVFGNKSNSNILKNLLPKNESPLAPTWILALAVLFILLQLLAIGMVY 324

Query: 209 AQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 268
           AQVAYEIMER+SADA++GVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG
Sbjct: 325 AQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 384

Query: 269 FIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKL 328
           FIPLDFILPMVLYNITHKPP TS+TYWVN+FIV AFSGAG++GCFASIRNLVLD+KKFKL
Sbjct: 385 FIPLDFILPMVLYNITHKPPVTSITYWVNVFIVAAFSGAGLLGCFASIRNLVLDSKKFKL 444

Query: 329 FSSHVV 335
           FSSHVV
Sbjct: 445 FSSHVV 450

BLAST of Lag0032649 vs. ExPASy TrEMBL
Match: A0A1S3ATJ4 (probable GABA transporter 2 OS=Cucumis melo OX=3656 GN=LOC103482813 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.0e-149
Identity = 277/306 (90.52%), Postives = 295/306 (96.41%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P GE+KLYHFIA+VTVGMI++SQ P+FHSLRYINFLSLLLSLAYAFF+ FASILAGTSDN
Sbjct: 144 PKGELKLYHFIAVVTVGMIMISQLPSFHSLRYINFLSLLLSLAYAFFIAFASILAGTSDN 203

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
           VP RDYSLEST S+RVFSAFTSISIFAAIFGNGILPEIQATLAPP  GKMVKGLIMCYIV
Sbjct: 204 VPPRDYSLESTPSARVFSAFTSISIFAAIFGNGILPEIQATLAPPTGGKMVKGLIMCYIV 263

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVY 208
           IFITFYS+AASGYWVFGNKSNSN+LKNLLP NESPLAPTW+L LAVLFILLQLLAIGMVY
Sbjct: 264 IFITFYSSAASGYWVFGNKSNSNILKNLLPKNESPLAPTWILALAVLFILLQLLAIGMVY 323

Query: 209 AQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 268
           AQVAYEIMER+SADA++GVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG
Sbjct: 324 AQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 383

Query: 269 FIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKL 328
           FIPLDFILPMVLYNITHKPP TS+TYWVN+FIV AFSGAG++GCFASIRNLVLD+KKFKL
Sbjct: 384 FIPLDFILPMVLYNITHKPPVTSITYWVNVFIVAAFSGAGLLGCFASIRNLVLDSKKFKL 443

Query: 329 FSSHVV 335
           FSSHVV
Sbjct: 444 FSSHVV 449

BLAST of Lag0032649 vs. ExPASy TrEMBL
Match: A0A0A0KK40 (Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G150970 PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.7e-148
Identity = 274/306 (89.54%), Postives = 293/306 (95.75%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P GE+KLYHFIA+VT+GMII+SQ P+FHSLRYINFLSLLLSLAYAFF+ FASILAGTSDN
Sbjct: 144 PKGELKLYHFIAVVTLGMIIISQLPSFHSLRYINFLSLLLSLAYAFFIAFASILAGTSDN 203

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
           VP RDYSLEST S+RVFSAFTSISIFAAIFGNGILPEIQATLAPP+ GKMVKGLIMCYIV
Sbjct: 204 VPPRDYSLESTPSARVFSAFTSISIFAAIFGNGILPEIQATLAPPIGGKMVKGLIMCYIV 263

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVY 208
           IFITFYS+AASGYWVFGNKSNSN+LKNLLP NE PLAPTW+L LAVLFILLQLLAIGMVY
Sbjct: 264 IFITFYSSAASGYWVFGNKSNSNILKNLLPKNEPPLAPTWILALAVLFILLQLLAIGMVY 323

Query: 209 AQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 268
           AQVAYEIMER+SADA++GVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG
Sbjct: 324 AQVAYEIMERRSADAKQGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 383

Query: 269 FIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKL 328
           FIPLDFILPMVLYNITHKPP TS+TYWVN+FIV AFSG G++GCFASIRNLVLD+KKF L
Sbjct: 384 FIPLDFILPMVLYNITHKPPVTSITYWVNVFIVAAFSGVGLLGCFASIRNLVLDSKKFNL 443

Query: 329 FSSHVV 335
           FSSHVV
Sbjct: 444 FSSHVV 449

BLAST of Lag0032649 vs. ExPASy TrEMBL
Match: A0A6J1ESG7 (probable GABA transporter 2 OS=Cucurbita moschata OX=3662 GN=LOC111435583 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.4e-147
Identity = 269/306 (87.91%), Postives = 295/306 (96.41%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P GE+KLYHFIAMVTVGMI++SQ P+FHSLRYINF+SLLLSLAYAF + FASILAGTSDN
Sbjct: 144 PEGELKLYHFIAMVTVGMILISQLPSFHSLRYINFVSLLLSLAYAFLIAFASILAGTSDN 203

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
           VP++ YSLESTLS+R+F+AFTSISIFAAIFGNGILPEIQATLAPPV GKMVKGLIMCYIV
Sbjct: 204 VPSKGYSLESTLSARLFTAFTSISIFAAIFGNGILPEIQATLAPPVGGKMVKGLIMCYIV 263

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVY 208
           IFITFYS+AASGYWVFGN+SNSN+LKNLLP N+SPLAPTW+L  AVLFILLQLLAIGMVY
Sbjct: 264 IFITFYSSAASGYWVFGNESNSNILKNLLPKNQSPLAPTWILLFAVLFILLQLLAIGMVY 323

Query: 209 AQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 268
           AQVAYEIME++SADA++GVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINS+VGAIG
Sbjct: 324 AQVAYEIMEKRSADAKQGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSIVGAIG 383

Query: 269 FIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKL 328
           FIPLDF+LPMVLYNITHKPP TS+TYWVN+FIVVAFSG G++GCFASIRNLVLDAKKFKL
Sbjct: 384 FIPLDFVLPMVLYNITHKPPVTSITYWVNVFIVVAFSGVGLLGCFASIRNLVLDAKKFKL 443

Query: 329 FSSHVV 335
           FSSHVV
Sbjct: 444 FSSHVV 449

BLAST of Lag0032649 vs. TAIR 10
Match: AT5G41800.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 414.1 bits (1063), Expect = 1.1e-115
Identity = 213/306 (69.61%), Postives = 252/306 (82.35%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P G +KLY FIAMVTV M+++SQ P+FHSLR+IN  SLLLSL Y F +V A I  G S N
Sbjct: 146 PQGTLKLYEFIAMVTVVMMVLSQLPSFHSLRHINCASLLLSLGYTFLVVGACINLGLSKN 205

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
            P R+YSLE + S +VFSAFTSISI AAIFGNGILPEIQATLAPP  GKM+KGL++CY V
Sbjct: 206 APKREYSLEHSDSGKVFSAFTSISIIAAIFGNGILPEIQATLAPPATGKMLKGLLLCYSV 265

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVY 208
           IF TFYSAA SGYWVFGN S+SN+LKNL+P     LAP  ++ LAV+F+LLQL AIG+VY
Sbjct: 266 IFFTFYSAAISGYWVFGNNSSSNILKNLMPDEGPTLAPIVVIGLAVIFVLLQLFAIGLVY 325

Query: 209 AQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIG 268
           +QVAYEIME+KSAD  KG+FS+RNL+PRLILRTLYM+ CGF AAM PFFGDIN+VVGA G
Sbjct: 326 SQVAYEIMEKKSADTTKGIFSKRNLVPRLILRTLYMAFCGFMAAMLPFFGDINAVVGAFG 385

Query: 269 FIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKL 328
           FIPLDF+LPM+LYN+T+KP   S TYW+N+ I+V F+ AG++G F+SIR LVLDA KFKL
Sbjct: 386 FIPLDFVLPMLLYNMTYKPTRRSFTYWINMTIMVVFTCAGLMGAFSSIRKLVLDANKFKL 445

Query: 329 FSSHVV 335
           FSS VV
Sbjct: 446 FSSEVV 451

BLAST of Lag0032649 vs. TAIR 10
Match: AT1G08230.2 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 142/303 (46.86%), Postives = 204/303 (67.33%), Query Frame = 0

Query: 29  PGGEVKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDN 88
           P GE+KL+ F+ +    +++++QFP+FHSLRYIN LSLLL L Y+     ASI  G   N
Sbjct: 147 PNGEMKLFEFVIIFGCLLLVLAQFPSFHSLRYINSLSLLLCLLYSASAAAASIYIGKEPN 206

Query: 89  VPARDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIV 148
            P +DY++     +RVF  F +++I A  +GNGI+PEIQAT++ PV GKM+KGL MCY+V
Sbjct: 207 APEKDYTIVGDPETRVFGIFNAMAIIATTYGNGIIPEIQATISAPVKGKMMKGLCMCYLV 266

Query: 149 IFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESP-LAPTWMLFLAVLFILLQLLAIGMV 208
           + +TF++ A +GYW FG K+N  +  N L    +    PTW +FL  LF +LQL A+ +V
Sbjct: 267 VIMTFFTVAITGYWAFGKKANGLIFTNFLNAETNHYFVPTWFIFLVNLFTVLQLSAVAVV 326

Query: 209 YAQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAI 268
           Y Q   +I+E   +D  K  FS RN+IPRL++R+L++ +    AAM PFFGD+NS++GA 
Sbjct: 327 YLQPINDILESVISDPTKKEFSIRNVIPRLVVRSLFVVMATIVAAMLPFFGDVNSLLGAF 386

Query: 269 GFIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFK 328
           GFIPLDF+LP+V +N T KP   S  +W+N  I V FS  G+I   A++R +++DA  +K
Sbjct: 387 GFIPLDFVLPVVFFNFTFKPSKKSFIFWINTVIAVVFSCLGVIAMVAAVRQIIIDANTYK 446

Query: 329 LFS 331
           LF+
Sbjct: 447 LFA 449

BLAST of Lag0032649 vs. TAIR 10
Match: AT1G48640.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 116.7 bits (291), Expect = 3.6e-26
Identity = 80/304 (26.32%), Postives = 156/304 (51.32%), Query Frame = 0

Query: 33  VKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDNVPAR 92
           ++L  FI +      ++S  P F+S+  ++ ++ ++SL+Y+     A+   G  ++V   
Sbjct: 163 IRLSFFIMIFASSHFVLSHLPNFNSISGVSLVAAVMSLSYSTIAWTATAAKGVQEDV--- 222

Query: 93  DYSLES-TLSSRVFSAFTSI-SIFAAIFGNGILPEIQATL----APPVAGKMVKGLIMCY 152
            Y  +S T +S V S FT +  I  A  G+ ++ EIQAT+    + P  G M +G+++ Y
Sbjct: 223 QYGYKSGTTASTVLSFFTGLGGIAFAYAGHNVVLEIQATIPSTPSNPSKGPMWRGVVVAY 282

Query: 153 IVIFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGM 212
           +V+ + ++  A  GY VFGN    N+L +L         P W +  A LF+++ ++    
Sbjct: 283 VVVALCYFPVALVGYGVFGNAVLDNVLMSL-------ETPVWAIATANLFVVMHVIGSYQ 342

Query: 213 VYAQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGA 272
           ++A   ++++E       K +  + + + R I+R +Y++L  F   M PFFG + +  G 
Sbjct: 343 IFAMPVFDMVE---TFLVKKLNFKPSTVLRFIVRNVYVALTMFIGIMIPFFGGLLAFFGG 402

Query: 273 IGFIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKF 331
             F P  + LP +++ + +KP   S+++W N   +V      I+     +R +++ +K +
Sbjct: 403 FAFAPTSYFLPCIMWLLIYKPKRFSLSWWTNWVCIVLGVVLMILSSIGGLRQIIIQSKDY 453

BLAST of Lag0032649 vs. TAIR 10
Match: AT1G71680.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 114.0 bits (284), Expect = 2.3e-25
Identity = 84/303 (27.72%), Postives = 151/303 (49.83%), Query Frame = 0

Query: 33  VKLYHFIAMVTVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDNVPAR 92
           ++  ++I       +++SQ P F+S++ ++ L+ L+S  Y+     ASI  GT ++ P+ 
Sbjct: 157 IRQTYYILGFAALQLVLSQSPDFNSIKIVSLLAALMSFLYSMIASVASIAKGT-EHRPST 216

Query: 93  DYSLESTLSSRVFSAFTSISIFAAIF-GNGILPEIQATL--APPVAGK--MVKGLIMCYI 152
                 T++S VF AF  I   A  F G+ ++ EIQAT+   P V  K  M KG+++ YI
Sbjct: 217 YGVRGDTVASMVFDAFNGIGTIAFAFAGHSVVLEIQATIPSTPEVPSKKPMWKGVVVAYI 276

Query: 153 VIFITFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMV 212
           ++ I +   A SGYW FG     ++L +L         P W++  A   + + ++    V
Sbjct: 277 IVIICYLFVAISGYWAFGAHVEDDVLISL-------ERPAWLIAAANFMVFIHVIGSYQV 336

Query: 213 YAQVAYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAI 272
           +A + ++ +E       K  F+    + RL+ R+ Y++L    A   PFFG +    G +
Sbjct: 337 FAMIVFDTIESYLVKTLK--FTPSTTL-RLVARSTYVALICLVAVCIPFFGGLLGFFGGL 396

Query: 273 GFIPLDFILPMVLYNITHKPPTTSMTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFK 331
            F    + LP +++ I  +P   S  +W +   +V      I+     +R+++L A+ +K
Sbjct: 397 VFSSTSYFLPCIIWLIMKRPKRFSAHWWCSWVAIVTGISIAILAPIGGMRHIILSARTYK 448

BLAST of Lag0032649 vs. TAIR 10
Match: AT2G36590.1 (proline transporter 3 )

HSP 1 Score: 111.7 bits (278), Expect = 1.2e-24
Identity = 86/302 (28.48%), Postives = 149/302 (49.34%), Query Frame = 0

Query: 33  VKLYHFIAMV-TVGMIIMSQFPTFHSLRYINFLSLLLSLAYAFFLVFASILAGTSDNVPA 92
           +KL HFIA+   +  +     P   +L     +S +LSL Y    +  S+  G     P+
Sbjct: 146 MKLPHFIAIAGLICAVFAIGIPHLSALGIWLAVSTILSLIYIVVAIVLSVKDGV--KAPS 205

Query: 93  RDYSLESTLSSRVFSAFTSISIFAAIFGNGILPEIQATLAPPVAGKMVKGLIMCYIVIFI 152
           RDY ++ +  S++F+   + +    +F  G+LPEIQAT+  PV   M+K L   + V  +
Sbjct: 206 RDYEIQGSPLSKLFTITGAAATLVFVFNTGMLPEIQATVKQPVVKNMMKALYFQFTVGVL 265

Query: 153 TFYSAAASGYWVFGNKSNSNLLKNLLPPNESPLAPTWMLFLAVLFILLQLLAIGMVYAQV 212
             ++    GYW +G+ ++  LL N+         P W+  LA +  +LQ +    ++A  
Sbjct: 266 PMFAVVFIGYWAYGSSTSPYLLNNV-------NGPLWVKALANISAILQSVISLHIFASP 325

Query: 213 AYEIMERKSADAQKGVFSRRNLIPRLILRTLYMSLCGFFAAMFPFFGDINSVVGAIGFIP 272
            YE M+ K    +    + +NL+ R++ R  Y+++    +A+ PF GD  S+ GA+   P
Sbjct: 326 TYEYMDTKFG-IKGNPLALKNLLFRIMARGGYIAVSTLLSALLPFLGDFMSLTGAVSTFP 385

Query: 273 LDFILPMVLYNITHKPPTTS---MTYWVNLFIVVAFSGAGIIGCFASIRNLVLDAKKFKL 331
           L FIL   +Y         +   + +W+N   VV FS   +    A++R + LD+K F +
Sbjct: 386 LTFILANHMYYKAKNNKLNTLQKLCHWLN---VVFFSLMSVAAAIAALRLIALDSKNFHV 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK06095.11.8e-16088.62putative GABA transporter 2 [Cucumis melo var. makuwa][more]
XP_038906705.11.1e-14990.85probable GABA transporter 2 [Benincasa hispida][more]
XP_008437380.14.1e-14990.52PREDICTED: probable GABA transporter 2 [Cucumis melo][more]
KAA0042692.14.1e-14990.52putative GABA transporter 2 [Cucumis melo var. makuwa][more]
XP_004143875.17.7e-14889.54probable GABA transporter 2 [Cucumis sativus] >KGN50030.1 hypothetical protein C... [more]
Match NameE-valueIdentityDescription
Q8L4X41.5e-11469.61Probable GABA transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g41800 PE=1 SV... [more]
F4HW022.4e-7546.86GABA transporter 1 OS=Arabidopsis thaliana OX=3702 GN=GAT1 PE=1 SV=1[more]
Q9C7335.0e-2526.32Lysine histidine transporter-like 1 OS=Arabidopsis thaliana OX=3702 GN=At1g48640... [more]
Q69LA18.6e-2529.93Probable proline transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=LOC_O... [more]
Q9C9J03.3e-2427.72Lysine histidine transporter-like 5 OS=Arabidopsis thaliana OX=3702 GN=At1g71680... [more]
Match NameE-valueIdentityDescription
A0A5D3C3Y38.6e-16188.62Putative GABA transporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A5A7TH102.0e-14990.52Putative GABA transporter 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A1S3ATJ42.0e-14990.52probable GABA transporter 2 OS=Cucumis melo OX=3656 GN=LOC103482813 PE=3 SV=1[more]
A0A0A0KK403.7e-14889.54Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G150970 PE... [more]
A0A6J1ESG72.4e-14787.91probable GABA transporter 2 OS=Cucurbita moschata OX=3662 GN=LOC111435583 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G41800.11.1e-11569.61Transmembrane amino acid transporter family protein [more]
AT1G08230.21.7e-7646.86Transmembrane amino acid transporter family protein [more]
AT1G48640.13.6e-2626.32Transmembrane amino acid transporter family protein [more]
AT1G71680.12.3e-2527.72Transmembrane amino acid transporter family protein [more]
AT2G36590.11.2e-2428.48proline transporter 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013057Amino acid transporter, transmembrane domainPFAMPF01490Aa_transcoord: 31..313
e-value: 6.2E-34
score: 117.4
NoneNo IPR availablePANTHERPTHR48017OS05G0424000 PROTEIN-RELATEDcoord: 29..328
NoneNo IPR availablePANTHERPTHR48017:SF60GABA TRANSPORTER 2 ISOFORM X1-RELATEDcoord: 29..328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032649.1Lag0032649.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006865 amino acid transport
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015293 symporter activity