Lag0032624 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032624
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionshort-chain dehydrogenase TIC 32, chloroplastic-like
Locationchr11: 35381265 .. 35383556 (-)
RNA-Seq ExpressionLag0032624
SyntenyLag0032624
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCCCTTCCTTCAACTACCTCGCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCACCGCCGACCTCGTCACCTCCTCCGCCCTCCCTCTCCCACTCCCTTCCATCACCGCCATCATCACCGGCGCCACCTCCGGCATCGGAGCCGAGACCGCCCGGGTCCTCGCCAAACGCGGCGTCCGTCTCGTCCTCCCCTCCCGCAACCTCAAGGCCGCCGAGGAAGCCAAGGCCCGGATCCAGTCCGAATCCCCCGACTCCCGAATCATCATCATGGCTCTCGATTTGAGCTCCCTCTCCTCCGTTCGAACCTTCGTCGCCCAGTTTCTATCTCTTGATCTCCCTCTCAATCTCCTCATCAACAACGCCGGCAAATTCTCCCACCAGCATGCACTTTCCGAGGACGGGATCGAGATGACCTTCGCCACCAATTACTTGGGTAACTATTTTTTTTTTCAAATAAAAACCATTTAAAAAATGGTGGAATTGAATTAAATTTTATTTTAATTTTGTTTTTCAGGTCATTTTCTGTTGACGAAACTGTTGGTGAATAAGATGGCGGAGACGGCGAAAGCGACGGGGGTTGAAGGGCGCATTGTGAACGTCTCGTCGAGCATCCACGGCTGGTTTTCCGGCGACATGATCAAATATCTCGCCCAGATAAGCCGAAACAAAAGGTGATTTAATTTCTTTTTCATTTTGACTTGTTATTATTATTATTATTTATTTATTTATTTAATTCTAATACACAGTTAGTTAATGTGGGTGGATTCCCAGCAGGAATTACGATGCGACACGTGCTTACGCGCTCTCTAAGCTTGCCAACGTTCTGCACACCCACGAACTTGCTCGCAGATTGAAGGTTCTTCTTTACATTTTATATTACTTTAATTACTCAATTTGCTCAATACTTATTATTATTATTATTTTATCTAAATTTGGACAGATGATCAAAGCTTAAACACAGACTAATTAATTTTTGTATTTTGTTTTGATTTAGCAAATGAAAGCTAATGTTACTGTGAACTGCGTTCATCCGGGAATCGTCAGAACTCGACTCACAAGAGATCGTGAAGGTTTCATCACTGGTACGAATCTTCTTTTATTTATTTTTTTTTCCGGGTAATATCGATATGAAAATTAAAAATAATTATTGAATAAAAACTGCAGATTTGGTGTTCTTTATGGCTTCCAAGCTGTTGAAGACAATCCCTCAGGTAAATAATCAAAACCCTCTCGATAATTTTGGTTAAATTGCCATTTTTATTTCTCGAAGTTGTGTATAATGGACTTCTTCACCTAAAAAAAAGGGTTATTTTTATCTTTTTAAAATAAATTTCTAATCTTATTTTATCCATTATTCTAAAGGCTAAATTATAAAAAAAATACTTTGAACTATGAGATTTGTTTCAAAAATGCCTCTAAACTTTGAAAATTTTCAAAAATACCTCTGAATTTTGAAAAATGTTTCAAATATACCCTAGAACTTTAATTTCTTTTTTAAATACCATTATTAAAAAATGTTTCTTCTTTTTTCTCCCTCTCATCGTAATTTTTTCCTCCATGAAAATTAATGCAAGAATTTACGCTGAGAGAGAGAAAAAAAAAAAACATTTTTCAATAATGATATTTTTAAAAAAATTTTTTTTTAAAGTTCGAAGGTATTTTTGAAACTAAAACTTCAACGGTTTCCATTCAAAAATTAACAGCAACGATATTTTTTAAACATTTTTCAAAGTTTAGTGACATTTTTTAAACAAACCACCCCATAATTTAGGGTATTTTTTTATAATTTAGCATATTCTAAATTTGCTACTAAAAACAATAAAAATGTAGAATACAACTCCGTTTTTGTAGACTTATTTTCAAATTTCTATTTTAAAATCACAATCCAACTCACAACTCACAATTTTGTTTTAAAAAAAAGGAAGAAAAAAACAAAAACCAAACCTTTTTCTTTCCTTCGAAGTTCACGTTTTCCGTTTGTTTCCCGAGAAAATTAAAAAGAAAAAAGAAAAAAGAAAAAAAGAAAAGAAAAGGCTATATTAGAACTGACACACAGGCCGTTCTGATCTTCTTCTTCCACAGGCGGCGGCAACGACATGCTACGTGGCGACGAGCCCGAGGCTAAGCCACGTCACCGGCAAGTATTTCTCAGACTGCAACGAAGCGTCGCCGTCGAAGCTCGCCGCCAGCCCCGCCGCAGCCGCCCGCCTCTGGTCCGCCTCCGAGATCATGGTCAACACAAACTCAAAACTCGTTTTCGATCCACACAATGCCTTAGACTAA

mRNA sequence

ATGCTCCCTTCCTTCAACTACCTCGCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCACCGCCGACCTCGTCACCTCCTCCGCCCTCCCTCTCCCACTCCCTTCCATCACCGCCATCATCACCGGCGCCACCTCCGGCATCGGAGCCGAGACCGCCCGGGTCCTCGCCAAACGCGGCGTCCGTCTCGTCCTCCCCTCCCGCAACCTCAAGGCCGCCGAGGAAGCCAAGGCCCGGATCCAGTCCGAATCCCCCGACTCCCGAATCATCATCATGGCTCTCGATTTGAGCTCCCTCTCCTCCGTTCGAACCTTCGTCGCCCAGTTTCTATCTCTTGATCTCCCTCTCAATCTCCTCATCAACAACGCCGGCAAATTCTCCCACCAGCATGCACTTTCCGAGGACGGGATCGAGATGACCTTCGCCACCAATTACTTGGGTCATTTTCTGTTGACGAAACTGTTGGTGAATAAGATGGCGGAGACGGCGAAAGCGACGGGGGTTGAAGGGCGCATTGTGAACGTCTCGTCGAGCATCCACGGCTGGTTTTCCGGCGACATGATCAAATATCTCGCCCAGATAAGCCGAAACAAAAGGAATTACGATGCGACACGTGCTTACGCGCTCTCTAAGCTTGCCAACGTTCTGCACACCCACGAACTTGCTCGCAGATTGAAGCAAATGAAAGCTAATGTTACTGTGAACTGCGTTCATCCGGGAATCGTCAGAACTCGACTCACAAGAGATCGTGAAGGTTTCATCACTGATTTGGTGTTCTTTATGGCTTCCAAGCTGTTGAAGACAATCCCTCAGGCGGCGGCAACGACATGCTACGTGGCGACGAGCCCGAGGCTAAGCCACGTCACCGGCAAGTATTTCTCAGACTGCAACGAAGCGTCGCCGTCGAAGCTCGCCGCCAGCCCCGCCGCAGCCGCCCGCCTCTGGTCCGCCTCCGAGATCATGGTCAACACAAACTCAAAACTCGTTTTCGATCCACACAATGCCTTAGACTAA

Coding sequence (CDS)

ATGCTCCCTTCCTTCAACTACCTCGCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCACCGCCGACCTCGTCACCTCCTCCGCCCTCCCTCTCCCACTCCCTTCCATCACCGCCATCATCACCGGCGCCACCTCCGGCATCGGAGCCGAGACCGCCCGGGTCCTCGCCAAACGCGGCGTCCGTCTCGTCCTCCCCTCCCGCAACCTCAAGGCCGCCGAGGAAGCCAAGGCCCGGATCCAGTCCGAATCCCCCGACTCCCGAATCATCATCATGGCTCTCGATTTGAGCTCCCTCTCCTCCGTTCGAACCTTCGTCGCCCAGTTTCTATCTCTTGATCTCCCTCTCAATCTCCTCATCAACAACGCCGGCAAATTCTCCCACCAGCATGCACTTTCCGAGGACGGGATCGAGATGACCTTCGCCACCAATTACTTGGGTCATTTTCTGTTGACGAAACTGTTGGTGAATAAGATGGCGGAGACGGCGAAAGCGACGGGGGTTGAAGGGCGCATTGTGAACGTCTCGTCGAGCATCCACGGCTGGTTTTCCGGCGACATGATCAAATATCTCGCCCAGATAAGCCGAAACAAAAGGAATTACGATGCGACACGTGCTTACGCGCTCTCTAAGCTTGCCAACGTTCTGCACACCCACGAACTTGCTCGCAGATTGAAGCAAATGAAAGCTAATGTTACTGTGAACTGCGTTCATCCGGGAATCGTCAGAACTCGACTCACAAGAGATCGTGAAGGTTTCATCACTGATTTGGTGTTCTTTATGGCTTCCAAGCTGTTGAAGACAATCCCTCAGGCGGCGGCAACGACATGCTACGTGGCGACGAGCCCGAGGCTAAGCCACGTCACCGGCAAGTATTTCTCAGACTGCAACGAAGCGTCGCCGTCGAAGCTCGCCGCCAGCCCCGCCGCAGCCGCCCGCCTCTGGTCCGCCTCCGAGATCATGGTCAACACAAACTCAAAACTCGTTTTCGATCCACACAATGCCTTAGACTAA

Protein sequence

MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKRGVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLLINNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASPSKLAASPAAAARLWSASEIMVNTNSKLVFDPHNALD
Homology
BLAST of Lag0032624 vs. NCBI nr
Match: KAG7035489.1 (Short-chain dehydrogenase TIC 32, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 579.7 bits (1493), Expect = 1.6e-161
Identity = 305/338 (90.24%), Postives = 319/338 (94.38%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VT+S+ PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
           GVRL+LPSRNLKAAEE KARI SE  DS+II+M LDLSSLSSV  FV QF SL+LPLNLL
Sbjct: 61  GVRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH HA+SEDGIEMTFATNYLGHFLLTKLLVN MA+TAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMADTAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQM+ANV+VNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPHNALD 339
           SPSKLAASP AAARLWSASEIMVNT SKLVFDP+N L+
Sbjct: 301 SPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 337

BLAST of Lag0032624 vs. NCBI nr
Match: XP_038875366.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 578.9 bits (1491), Expect = 2.8e-161
Identity = 304/338 (89.94%), Postives = 318/338 (94.08%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML S NYL+GSPGPSGFGSKSTA+ VTSSA P  LPS TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLLSLNYLSGSPGPSGFGSKSTAEHVTSSA-PFLLPSFTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
           G RL+LPSRNLKAAEE KARI+SE  DS II+M+LDLSSLSSVRTFVAQF SL+LPLNLL
Sbjct: 61  GARLILPSRNLKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVAQFESLNLPLNLL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH+HA+SEDGIEMTFATNYLGHFLLTKLLVNKM ETAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNKMVETAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHP 240
           HGWFSGD+IKYL QISRNKRNYDATRAYALSKLANVLHTHEL RRLKQM ANVTVNCVHP
Sbjct: 181 HGWFSGDIIKYLGQISRNKRNYDATRAYALSKLANVLHTHELGRRLKQMGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAT+PRLSHVTGKYF+DCNE+
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLSHVTGKYFADCNES 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPHNALD 339
           SPSKLAASP  AARLWSASEIMVN NSKLVFDP+NALD
Sbjct: 301 SPSKLAASPTEAARLWSASEIMVNANSKLVFDPNNALD 337

BLAST of Lag0032624 vs. NCBI nr
Match: XP_023534460.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578.6 bits (1490), Expect = 3.6e-161
Identity = 305/338 (90.24%), Postives = 318/338 (94.08%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VT+S+ PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
           GVRL+LPSRNLKAAEE KARI SE  DS+II+M LDLSSLSSV  FV QF SL+LPLNLL
Sbjct: 61  GVRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH HA+SEDGIEMTFATNYLGHFLLTKLLVN MAETAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMAETAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQM+ANV+VNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRL HVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLIHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPHNALD 339
           SPSKLAASP AAARLWSASEIMVNT SKLVFDP+N L+
Sbjct: 301 SPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 337

BLAST of Lag0032624 vs. NCBI nr
Match: XP_022957741.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 577.8 bits (1488), Expect = 6.2e-161
Identity = 305/338 (90.24%), Postives = 318/338 (94.08%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VT+S+ PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
            VRL+LPSRNLKAAEE KARI SE  DS+II+M LDLSSLSSV  FV QF SL+LPLNLL
Sbjct: 61  RVRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH HA+SEDGIEMTFATNYLGHFLLTKLLVN MAETAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMAETAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQM+ANV+VNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPHNALD 339
           SPSKLAASP AAARLWSASEIMVNT SKLVFDP+N L+
Sbjct: 301 SPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 337

BLAST of Lag0032624 vs. NCBI nr
Match: XP_022995788.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 575.5 bits (1482), Expect = 3.1e-160
Identity = 302/338 (89.35%), Postives = 317/338 (93.79%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VT+S+ PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
           GVRL+LPSRNLKAAEE K+RI SE  DS+II+M LDLSSLSSV  FV QF SL+LPLNLL
Sbjct: 61  GVRLILPSRNLKAAEETKSRILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH+HA+SEDGIEMTFATNYLGHFLLTKLLVN M ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKETGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQM+ANVTVNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPHNALD 339
           SPSKLAASP AAARLWS SEIMVN+ SKLVFDP+N L+
Sbjct: 301 SPSKLAASPTAAARLWSTSEIMVNSKSKLVFDPNNGLE 337

BLAST of Lag0032624 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.8e-70
Identity = 156/323 (48.30%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 5   FNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKRGVRL 64
           +  + G  G SG+GS STA+ VT S   +    +TAIITG TSGIG E ARVL  RG  +
Sbjct: 4   YGMVTGKAGKSGYGSASTAEDVTHS---IDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 63

Query: 65  VLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLLINNA 124
           ++ SRN KAA ++K  I    P++RI  + LDLSS+ SVR+F+ QFL+L++PLN+LINNA
Sbjct: 64  IIASRNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNA 123

Query: 125 GKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIH--G 184
           G       LSEDGIE  FATN++GHFLLT LL++KM  +A+ +G+EGRIVN+SS  H   
Sbjct: 124 GVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYT 183

Query: 185 WFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHPGI 244
           +  G M  Y+    R    Y   +AY  SKLAN+LH++ L+R+L++   N+T+N VHPG+
Sbjct: 184 YTEGIMFDYINDPDR----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGL 243

Query: 245 VRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASP 304
           + T L R   G    ++  M+  L K IPQ AATTCYVA  P L  VTGKYF+DCN  +P
Sbjct: 244 ITTNLFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTP 303

Query: 305 SKLAASPAAAARLWSASEIMVNT 326
           S  A     A +LW  S  +V +
Sbjct: 304 SNFAIDTTLADKLWDFSIKLVES 318

BLAST of Lag0032624 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 4.0e-70
Identity = 155/323 (47.99%), Postives = 209/323 (64.71%), Query Frame = 0

Query: 5   FNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKRGVRL 64
           +  + G  G SG+GS STA+ VT S   +    +TAIITG TSGIG E ARVL  RG  +
Sbjct: 4   YGMVTGKAGKSGYGSASTAEDVTHS---IDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 63

Query: 65  VLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLLINNA 124
           ++ +RN KAA ++K  I    P++RI  + LDLSS+ SVR+F+ QFL+L++PLN+LINNA
Sbjct: 64  IIAARNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNA 123

Query: 125 GKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIH--G 184
           G       LSEDGIE  FATN++GHFLLT LL++KM  +A+ +G+EGRIVN+SS  H   
Sbjct: 124 GVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYT 183

Query: 185 WFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHPGI 244
           +  G M  Y+    R    Y   +AY  SKLAN+LH++ L+R+L++   N+T+N VHPG+
Sbjct: 184 YTEGIMFDYINDPDR----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGL 243

Query: 245 VRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASP 304
           + T L R   G    ++  M+  L K IPQ AATTCYVA  P L  VTGKYF+DCN  +P
Sbjct: 244 ITTNLFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTP 303

Query: 305 SKLAASPAAAARLWSASEIMVNT 326
           S  A     A +LW  S  +V++
Sbjct: 304 SNFAIDTTLADKLWDFSIKLVDS 318

BLAST of Lag0032624 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.5e-64
Identity = 142/318 (44.65%), Postives = 204/318 (64.15%), Query Frame = 0

Query: 10  GSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKRGVRLVLPSR 69
           GS G SGF S+STA+ VT     +    +TAI+TGA+SGIG ETARVL+ RGV +V+  R
Sbjct: 5   GSKGASGFSSRSTAEEVTHG---VDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAVR 64

Query: 70  NLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLLINNAGKFSH 129
           N  +  + K  I  + P +++ +M LDLSS+ SVR F +++ S  LPLNLLINNAG  + 
Sbjct: 65  NTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMAC 124

Query: 130 QHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWFSGDMI 189
              LS+D IE+ FATN+LGHFLLTKLL++ M  T++ +  EGRIVN+SS  H +   + +
Sbjct: 125 PFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEGV 184

Query: 190 KYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHPGIVRTRLTR 249
           ++      +K +Y + RAY  SKL NVLH +EL ++LK+   N+T N +HPG + T L R
Sbjct: 185 RF--DKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGR 244

Query: 250 DREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASPSKLAASP 309
               ++   V  +A  +LK++PQ AATTCYVA +P+++ V+G+YF D N A P  L    
Sbjct: 245 YFNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDT 304

Query: 310 AAAARLWSASEIMVNTNS 328
             A ++W  S  + ++ S
Sbjct: 305 ELAKKVWDFSTKLTDSQS 317

BLAST of Lag0032624 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.3e-57
Identity = 132/307 (43.00%), Postives = 189/307 (61.56%), Query Frame = 0

Query: 13  GPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKRGVRLVLPSRNLK 72
           G SGF   STA+ VT     +    +TAI+TGA+SGIGAET RVLA RG  +++  RN+ 
Sbjct: 9   GVSGFSGSSTAEQVTHG---IDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMV 68

Query: 73  AAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLLINNAGKFSHQHA 132
           AA++ K  I  + P +++  + LDLSSL SV+ F ++F S   PLN+LINNAG  +    
Sbjct: 69  AAKDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFK 128

Query: 133 LSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWFSGDMIKYL 192
           LS+D IE+ FATN++GHFLLT LL++ M +T + +  EGRIVNV+S  H +   + I++ 
Sbjct: 129 LSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRF- 188

Query: 193 AQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHPGIVRTRLTRDRE 252
                ++ +Y+  RAY  SKLANVLH ++L + LK+   N+T N +HPG + T L R   
Sbjct: 189 -DKINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRHNS 248

Query: 253 GFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASPSKLAASPAAA 312
             +  L+  +   +LK + Q AATTCYVA  P++  V+G+YFSD N    +        A
Sbjct: 249 A-VNGLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLA 308

Query: 313 ARLWSAS 320
            +LW  S
Sbjct: 309 KKLWDFS 309

BLAST of Lag0032624 vs. ExPASy Swiss-Prot
Match: P59837 (Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 4.2e-43
Identity = 114/279 (40.86%), Postives = 165/279 (59.14%), Query Frame = 0

Query: 41  IITGATSGIGAETARVLAKRGVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSL 100
           +ITGA +GIG ETAR LA+RG R+ +  R++   E A + IQ+++ +S++++  LDLS  
Sbjct: 43  VITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIQADTKNSQVLVRKLDLSDT 102

Query: 101 SSVRTFVAQFLSLDLPLNLLINNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKM 160
            S+R F   FL+ +  L++LINNAG     ++ + DG E   A N+LGHFLLT LL+ ++
Sbjct: 103 KSIRAFAEGFLAEEKQLHILINNAGVMLCPYSKTADGFETHLAVNHLGHFLLTHLLLGRL 162

Query: 161 AETAKATGVEGRIVNVSSSIHGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTH 220
            E+A A     R+VN+SS  H      + K      +  + Y+   AY  SKLANVL T 
Sbjct: 163 KESAPA-----RVVNLSSVAH-----HLGKIRFHDLQGDKYYNLGFAYCHSKLANVLFTR 222

Query: 221 ELARRLKQMKANVTVNCVHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYV 280
           ELA+RLK     VT   VHPGIVR++L   R  F+  L++ + S  LKT  + A T+ + 
Sbjct: 223 ELAKRLK--GTGVTTYAVHPGIVRSKLV--RHSFLLCLLWRLFSPFLKTTWEGAQTSLHC 282

Query: 281 ATSPRLSHVTGKYFSDCNEASPSKLAASPAAAARLWSAS 320
           A +  L  ++GKYFSDC +   S  A +   A RLW+ S
Sbjct: 283 ALAEGLEPLSGKYFSDCKKTWVSPRARNNKTAERLWNVS 307

BLAST of Lag0032624 vs. ExPASy TrEMBL
Match: A0A6J1H032 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459194 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 3.0e-161
Identity = 305/338 (90.24%), Postives = 318/338 (94.08%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VT+S+ PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
            VRL+LPSRNLKAAEE KARI SE  DS+II+M LDLSSLSSV  FV QF SL+LPLNLL
Sbjct: 61  RVRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH HA+SEDGIEMTFATNYLGHFLLTKLLVN MAETAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMAETAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQM+ANV+VNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPHNALD 339
           SPSKLAASP AAARLWSASEIMVNT SKLVFDP+N L+
Sbjct: 301 SPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 337

BLAST of Lag0032624 vs. ExPASy TrEMBL
Match: A0A6J1K910 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491218 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.5e-160
Identity = 302/338 (89.35%), Postives = 317/338 (93.79%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VT+S+ PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
           GVRL+LPSRNLKAAEE K+RI SE  DS+II+M LDLSSLSSV  FV QF SL+LPLNLL
Sbjct: 61  GVRLILPSRNLKAAEETKSRILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH+HA+SEDGIEMTFATNYLGHFLLTKLLVN M ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKETGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQM+ANVTVNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPHNALD 339
           SPSKLAASP AAARLWS SEIMVN+ SKLVFDP+N L+
Sbjct: 301 SPSKLAASPTAAARLWSTSEIMVNSKSKLVFDPNNGLE 337

BLAST of Lag0032624 vs. ExPASy TrEMBL
Match: A0A6J1H1D2 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459194 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 7.4e-160
Identity = 305/339 (89.97%), Postives = 318/339 (93.81%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VT+S+ PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
            VRL+LPSRNLKAAEE KARI SE  DS+II+M LDLSSLSSV  FV QF SL+LPLNLL
Sbjct: 61  RVRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH HA+SEDGIEMTFATNYLGHFLLTKLLVN MAETAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMAETAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNK-RNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVH 240
           HGWFSGDMIKYL QISRNK RNYDATRAYALSKLANVLHTHELARRLKQM+ANV+VNCVH
Sbjct: 181 HGWFSGDMIKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVH 240

Query: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE 300
           PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE
Sbjct: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE 300

Query: 301 ASPSKLAASPAAAARLWSASEIMVNTNSKLVFDPHNALD 339
           ASPSKLAASP AAARLWSASEIMVNT SKLVFDP+N L+
Sbjct: 301 ASPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 338

BLAST of Lag0032624 vs. ExPASy TrEMBL
Match: A0A6J1JZY1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491218 PE=3 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.6e-159
Identity = 302/339 (89.09%), Postives = 317/339 (93.51%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VT+S+ PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
           GVRL+LPSRNLKAAEE K+RI SE  DS+II+M LDLSSLSSV  FV QF SL+LPLNLL
Sbjct: 61  GVRLILPSRNLKAAEETKSRILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH+HA+SEDGIEMTFATNYLGHFLLTKLLVN M ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKETGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNK-RNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVH 240
           HGWFSGDMIKYL QISRNK RNYDATRAYALSKLANVLHTHELARRLKQM+ANVTVNCVH
Sbjct: 181 HGWFSGDMIKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVH 240

Query: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE 300
           PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE
Sbjct: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE 300

Query: 301 ASPSKLAASPAAAARLWSASEIMVNTNSKLVFDPHNALD 339
           ASPSKLAASP AAARLWS SEIMVN+ SKLVFDP+N L+
Sbjct: 301 ASPSKLAASPTAAARLWSTSEIMVNSKSKLVFDPNNGLE 338

BLAST of Lag0032624 vs. ExPASy TrEMBL
Match: A0A5D3C462 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001850 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.9e-156
Identity = 294/338 (86.98%), Postives = 315/338 (93.20%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SFNYL+GSPGPSGFGSKSTA+ VTSS   LP PS TAIITGATSGIGAETAR+LAKR
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVTSS---LP-PSFTAIITGATSGIGAETARILAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
           G RL+LPSRNLKAAEE KARI+SE  DS II+M+LDLSSLSSVRTFV+QF SL+LPLNLL
Sbjct: 61  GARLILPSRNLKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH+HA++EDGIEMTFATNYLGHFLLTKLLVNKM ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHP 240
           H WFSGD++KYL QISRNKRNYDATRAYALSKLANVLHTHELARR KQM ANVTVNCVHP
Sbjct: 181 HSWFSGDILKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAT+PRL HVTGKYF DCNE+
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNES 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPHNALD 339
           SPSKLAASP+ AARLWSASEIMVN +SKL+FDP+NAL+
Sbjct: 301 SPSKLAASPSEAARLWSASEIMVNASSKLLFDPNNALE 334

BLAST of Lag0032624 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 459.1 bits (1180), Expect = 3.0e-129
Identity = 236/333 (70.87%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           M+ +  YL G+ G SGFGSKSTA+ VT +     L SITA+ITGATSGIGAETARVLAKR
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENC---DLRSITAVITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
           G RL+ P+RN+KAAEEAK RI SE P++ I++M LDLSS++SVR FVA F SLDLPLNLL
Sbjct: 61  GARLIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGK +H+HA+SEDGIEMTFATNYLGHFLLT LL+NKM +TA+ TGV+GRIVNV+S I
Sbjct: 121 INNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHP 240
           HGWFSGD+I+YL  IS+ K  +DATRAYALSKLANVLHT EL+ RL+++ ANVTVNCVHP
Sbjct: 181 HGWFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           G+VRTRLTRDREG +TDLVFF+ASKL+KT+PQAAATTCYVAT+PRL +V+GKYF+DCNE 
Sbjct: 241 GVVRTRLTRDREGLLTDLVFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNET 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDP 334
           +PS L  + + A +LW+ASEI+V  +SK  FDP
Sbjct: 301 TPSGLGTNSSEATKLWAASEILVTQHSKTSFDP 330

BLAST of Lag0032624 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 451.4 bits (1160), Expect = 6.2e-127
Identity = 236/324 (72.84%), Postives = 275/324 (84.88%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           M+ +  +L GS GPSGFGS+STAD VT ++    L S+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNS---DLRSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
           G RLVLP+R++K AEE KARI SE PD+ II+M LDLSSL+SVR FV  F SL+LPLN+L
Sbjct: 61  GARLVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNIL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGK++H+HALSEDG+EMTFATNYLGHFLLTKLL+ KM ETA  TGV+GRIVNV+S +
Sbjct: 121 INNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVV 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHP 240
           H WFSGDM++YLA ISRN RNYDATRAYALSKLANVLHT EL+R L +M ANVT NCVHP
Sbjct: 181 HSWFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIV+TRLTRDREG +TDLVFF+ SKLLK++PQAAATTCYVATSPRL +V GKYFSDCNEA
Sbjct: 241 GIVKTRLTRDREGVVTDLVFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVN 325
             SK  +    A RLW+AS+++V+
Sbjct: 301 RSSKSGSCNLKAQRLWTASDLLVS 321

BLAST of Lag0032624 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 320.5 bits (820), Expect = 1.7e-87
Identity = 179/327 (54.74%), Postives = 230/327 (70.34%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           M  +  YLAG  GP+GFGS+STA+ VT  +   P   +TAIITG TSGIGAETARVLAKR
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSF-FPCSHLTAIITGGTSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
           GVR+V+  R++K AE  K RI  E+P++ II+  +DLSSLSSV  F +QFLS DLPLN+L
Sbjct: 61  GVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAG FS     SE+ IE+TFATN+LGH+LLT++L+ KM +TA+ +G+EGRI+N+SS I
Sbjct: 121 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 180

Query: 181 HGWFSGDMI---KYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNC 240
           H W   D     K L  ISR    Y+ TRAYA SKLA +LH   L+++LK   ANVT+N 
Sbjct: 181 HNWVKPDCFSFPKLLHPISR----YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINA 240

Query: 241 VHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDC 300
           VHPGIV+T + R  +G  TD +F +ASKLLK+I Q AATTCYVA S     ++GKYF+DC
Sbjct: 241 VHPGIVKTGIIRAHKGLFTDSLFLIASKLLKSISQGAATTCYVALSNETKGLSGKYFADC 300

Query: 301 NEASPSKLAASPAAAARLWSASEIMVN 325
           NE + S LA     A +L + S  +++
Sbjct: 301 NETNCSDLANDEYVALKLCTQSRALIH 322

BLAST of Lag0032624 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 284.3 bits (726), Expect = 1.3e-76
Identity = 161/324 (49.69%), Postives = 218/324 (67.28%), Query Frame = 0

Query: 5   FNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKRGVRL 64
           ++ + G  GPSGFGS STA+ VT     +   ++TAIITG T GIG ETARVL+KRG  +
Sbjct: 4   YSLITGRRGPSGFGSASTAEEVTQG---IDATNLTAIITGGTGGIGMETARVLSKRGAHV 63

Query: 65  VLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLLINNA 124
           V+ +RN+ AAE AK  I  ++ ++R+ ++ LDLSS+ S++ FV +F +L LPLNLLINNA
Sbjct: 64  VIGARNMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNA 123

Query: 125 GKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWF 184
           G     + LSEDGIE+ FATN++GHFLLT LL++ M  TAK +GVEGRI+NVSS  H + 
Sbjct: 124 GVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYT 183

Query: 185 SGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNCVHPGIVR 244
             + I++      +  +Y   RAY  SKLAN+LH +EL+R+L++   N+T N VHPG++ 
Sbjct: 184 YQEGIQF--DSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLIL 243

Query: 245 TRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASPSK 304
           T L +     +  L FF +  L K IPQ AATTCYVA  P +  VTGKYF+DCNE +PSK
Sbjct: 244 TNLFQHTALLMRFLKFF-SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSK 303

Query: 305 LAASPAAAARLWSASEIMVNTNSK 329
           LA     A +LW  S  ++N+ SK
Sbjct: 304 LARDETLAQKLWDFSVKLINSVSK 321

BLAST of Lag0032624 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 280.4 bits (716), Expect = 1.9e-75
Identity = 156/275 (56.73%), Postives = 198/275 (72.00%), Query Frame = 0

Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTSSALPLPLPSITAIITGATSGIGAETARVLAKR 60
           M  +  YLAG  GP+GFGS+STA+ VT  +   P   +TAIITG TSGIGAETARVLAKR
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSF-FPCSHLTAIITGGTSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLDLPLNLL 120
           GVR+V+  R++K AE  K RI  E+P++ II+  +DLSSLSSV  F +QFLS DLPLN+L
Sbjct: 61  GVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 120

Query: 121 INNAGKFSHQHALSEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAG FS     SE+ IE+TFATN+LGH+LLT++L+ KM +TA+ +G+EGRI+N+SS I
Sbjct: 121 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 180

Query: 181 HGWFSGDMI---KYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMKANVTVNC 240
           H W   D     K L  ISR    Y+ TRAYA SKLA +LH   L+++LK   ANVT+N 
Sbjct: 181 HNWVKPDCFSFPKLLHPISR----YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINA 240

Query: 241 VHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQ 273
           VHPGIV+T + R  +G  TD +F +ASKLLK+I Q
Sbjct: 241 VHPGIVKTGIIRAHKGLFTDSLFLIASKLLKSISQ 270

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035489.11.6e-16190.24Short-chain dehydrogenase TIC 32, chloroplastic [Cucurbita argyrosperma subsp. a... [more]
XP_038875366.12.8e-16189.94short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa his... [more]
XP_023534460.13.6e-16190.24short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo ... [more]
XP_022957741.16.2e-16190.24short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita mosch... [more]
XP_022995788.13.1e-16089.35short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
A0A078IS661.8e-7048.30Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A0A078ISJ64.0e-7047.99Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A2RVM01.5e-6444.65Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q6RVV42.3e-5743.00Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3... [more]
P598374.2e-4340.86Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H0323.0e-16190.24short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita mos... [more]
A0A6J1K9101.5e-16089.35short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max... [more]
A0A6J1H1D27.4e-16089.97short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos... [more]
A0A6J1JZY13.6e-15989.09short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max... [more]
A0A5D3C4622.9e-15686.98Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT4G24050.13.0e-12970.87NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G64590.16.2e-12772.84NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G50130.11.7e-8754.74NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G02540.11.3e-7649.69NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G50130.21.9e-7556.73NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 208..227
score: 31.71
coord: 115..126
score: 44.39
coord: 171..179
score: 54.94
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 39..56
score: 45.52
coord: 208..227
score: 30.81
coord: 165..181
score: 29.05
coord: 115..126
score: 42.05
coord: 231..248
score: 37.54
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 39..250
e-value: 1.4E-32
score: 112.7
NoneNo IPR availableGENE3D3.40.50.720coord: 35..328
e-value: 2.6E-75
score: 255.2
NoneNo IPR availablePANTHERPTHR24320RETINOL DEHYDROGENASEcoord: 9..328
NoneNo IPR availablePANTHERPTHR24320:SF210MITOCHONDRIAL PYRUVATE CARRIERcoord: 9..328
NoneNo IPR availableCDDcd05327retinol-DH_like_SDR_c_likecoord: 39..316
e-value: 4.29369E-92
score: 274.102
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 39..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032624.1Lag0032624.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity