Lag0032618 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032618
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionGlutamine-dependent NAD(+) synthetase
Locationchr11: 35325952 .. 35331318 (-)
RNA-Seq ExpressionLag0032618
SyntenyLag0032618
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGTTATTGAAAGTGGCAACCTGTAATTTGAATCAATGGGCTATGGATTTCGATTGTAATGTGAAGCACATCAAAGAGTCCATTGACGAGGCTAAACGTGCTGGGGCTGTCATTAGACTTGGTCCGGAACTCGAGATTACTGGATATGGCTGTGAAGACCATTTCTTGGAGCTCGACACTGTCGCTCATGCGTGAGTTTCCTGCTCGTTGTATCTCAATTTGTTTGTCCTATGCGAAAATGTCTCTAATTGGTTATGGATTATTGTTTTTTGTCTTTCCAACAAAAGGCAATTTGTGGGGTCCTTTACAAACTTCATACTTGGAAGTGCATTACTAGACATATTTGATGGTTTCTAGCTTCTGGGGTTGCTCAAATTTCTTGTTTTAGGATACTTTTGCAGATGGGAATGCTTGAAGGATATACTGTTGGGTCCTTGGACTGATGGGATATTGTGCAGCATTGGCATGCCTGTTATTAAAGACTCTGAGCGTTACAATTGTCAAGTGCTATGCTATAATAGGAAAATTATTATGATACGCCCAAAGATGTGGCTTGCAAATGATGGGAATTACCGGGAGCTCCGCTGGTTCACAGCATGGAAGCTAAAGGACAAACTTGTGGACTTTCAGCTGCCAAAAGATATTTCTGAGGCTCTGTCACAAGCGTCTGCGCCTTTTGGCTATGGATATATTCAGTTTCTAGACACGTGAGTGCCAAAATTACTGGTTTTATGTATTGTTGTTCTTTTTGCTAGTCCAAAATTCATATGCAAGAATATGTAACTTTTGATTCCTGCATATCAATACGTGATAATTGGATGTTGGATGTTTTGTTCGTAATTATATTTATAGAATAATACAAGGGTCTGATGCCTAGTGTGTTCCATCTTGTTAGAGCTGTTGCTGCTGAAGTTTGTGAAGAACTATTTACGCCTACTCCTCCTCATGCTGAACTTGCTTTGAATGGGGTCGAAGTATTTATGAACGCTAGTGGAAGTCATCACCAACTAAGAAAACTTGACGTTCGTCTTCGTGCTTTTACAGGTGCTACTCATAATCGTGGTGGAGTTTACATGTATAGTAACCACCAGGGTTGTGACGGTGGTCGCCTTTACTATGGTATGCACCACTCTTTTGCATAGAATATTGTAGTGTTGCTGGAATTGCATTTCTTTCGTGGTATTCTTAGGACATTCATGATATGAAAATATTTATTTGAATGTACTCTATGCGGGTCCATTTTGAATGTTCACGTGCCTTTATGTGCAGCATATTGTGTGTTATGTTTTTTGTGCATATTGACATTTGAAGTTTGCAGAAGAAGACAAAGGATTTATTGAAGTTTATGTTTTGCTGTAGATGGATGTGCCTGTGTTGTTATAAACGGTGATCTGGTAGCTCAAGGCTCACAGTTTTCTTTGAAGGATGTTGAGGTTGTGGTTGCCCATGTAGATCTTGATGCGGTATGAAAATACTCTCTAGTTTCCTTGGACATCTTCAACAATGTGAGCCATGGAATGATTTACTTGATCCCTGTATCTTATATTGATTAACACGAGTTACTCCCTTTTCCCTCCATCATAACTCATAAGTTAGTATCATTTTAAAGGTCACTTGTTGAATCTAAGTTGTGGATGTTTTCTATCAAGGGTGCTTAGAGGTAGATATTTGGGAAGACCCTTCAAAGTCTTGGGTTAAAACGGTAGATAGAAAATATAATAATTTAAAATTCTTGTTGTCACTCAAAACTGACCTGAAATATATGGGGTAGGGTAGGCTATGAACATAATGAAGGCGTGGAGCCCTGATACCCCAATTGTTAAAAACATCGGTGGGAGTTTTCTTTTTCTTTCTTTCTATTTTTCTGAACATTTGGTTTTAAGAAGTCTGCTTGCTGACAGGTTGCTAGTCTTAGAGGATCTATCAGCAGCTTTCAAGAACAAGCAAGCTATAAAACTAAAGTTCCTTCTGTGGAAGCTCCATACAGTCTATGTCAACCATTCAACCTCAAAACTTCCCTTTCAAGTCCACTTGAGGTCAGTTACTAATTCAGTATCATTTCAGTATTTTAGTTCATTATGTCAAAGGCTGATCTTTGGACCTGGTTGATGGTTTATAGATTAACTATCACTGTGCGGAAGAGGAAATAGCCTTTGGACCTGGTTGCTGGTTATGGGACTATTTAAGACGAAGTGGAGCTTCTGGATTTCTGCTTCCTCTTTCTGGTGGAGCTGATAGCTCCTCTGTTGCAGCCATAGTTGGCTGCATGTGCCAGCTTGTCGTAAAAGGTTAGTTTTTATTAGCCATGGACATAAATAGAGTTGCTGTGTTCAGGTATCTCTATTTTGCAAGTTCAGTTAAAGTAGGAGACGGCCGGGAGCTGTCTTTATGATCAAAGATTTGTTTTTTAGGATGAGAAAAGTGAGCAAGCGACTGTTGATTGAGTCATCTGATGTTTGATTATTGATATGATAGTATCTCTACCACATAAGACAGTAGGTAACAACTCAATGGTGAATAAATGATTTGGAAGGAACCTGTATTCTACATTAACTGAAATGTGACTGAATGATGATTGTCCATATCTGGAAGAGTATAAGGAATGACGTATAATTACAAAATTCTGTCGATACCCAATAAGAAGCATGGGGTTTTGCTAATTGGAACACTTCCCACCAATCACTCACACTTGTATTCTTTTGGTTGTTTCTATGGATTGGTTTGCAGATATTGCAAATGGAGACGAACAGGTGAAAGCTGATGCAATACGGATTGGACATTATGCTGATGGAGAGTTCCCAACTGATAGCAGAGAATTTGCCAGACGCATTTTTTATACTGTATTTATGGGTTCTGAAAATAGGTGAGTAGAATGGTTAGGTTCATTTTTAATAACCATAGGTGACCCAACTAGAACACACGCACATCAACTGGATCCCATGGGCGACCTGCTTGACCTTACTATAATATAGCAATGCTTTGTCCATGACTTCATCCTGATTTATCACCTGGATTTTAACATGAACATGAATCTGAACTCAAAAACCCAGTTGGAGTCCTGATACGTTTATTTATTTTAATCTTTTAATCAATAGGTCATACACTTGATCTTTTTGTAGAGCTCAAACTCTGGTATCATTCATTGACCATGCTCGCTCCTTGTAGCTCTGAAGCAACACGTTCAAGAGCAAAGGTTCTAGCAGATGAGATTGGTTCATGGCATCTTGATGTTTCCATCGATGGGATTATTTCAGCACTTTTGTCTTTGTTCCAAACTCTCACTGGAAAACGTCCACGGTATAAGGTGGAAATATATTTTTCTTCTTCGAAATTTGTTGTGTTGTCTCAAGGATGGGGTTAGGTTCTTTTCTGCAAAATATATTTTCTTCACGGTTAGGTTGAAGTTGATTTTATGCCCATCCTTAAGATATTTTAACTGTTCTGATTACTAGCTATTTATGTCTTAAGCTCCATCATTGTCTTGTTGTGCAGGTAGATGGGGGATCTAATATTGAAAATTTAGGTCTGCAAAATATTCAAGCTCGAATCAGGATGGTTCTGGCATTTATGTTAGCGTCACTCTTACCTTGGGTACATAGTAAACCCGGATTCTACCTTGTTCTTGGTAGCTCCAATGTCGATGAAGGATTGCGTGGTTACTTAACAAAGGTTCGTTATTATTTGAACAATTTCTTTTTCCTTCTTGACTTTCTATAGACGTCGACTAGTTAAGCTGCCATGTTATCTGTTTTTATGAGTATCTCATGTTTGGATTCTCGACACAGTATGATTGCAGTTCAGCAGATATAAATCCAATTGGAAGCATCAGTAAGCAGGATCTCCGGGCATTTCTTAGATGGGCTGCCACCCATCTTGGCTACTCATCGTTGGCGGATATCGAAGCAGCTCCGCCTACAGCTGAGCTGGAGCCAATTCGTTCCAACTACAGCCAGGTTCTTGTCTTCCACATCTCACATTTTTCTTGACGTCTGAAAACGTTCTCTTCAGATGCATTTTATCCTAGAGCTGGATCTGACATTTTTAAAACTGTTTCTGATGTTACTATTATTGGATCTATTCTTGCAACCTATGGTTTCCCTTACGGGTGTAACTTAATCGATCGGGTCGGTTTTGTTTTGTCTCTAATCAAACTATTGGTCCAAATCAATGACGGACCAACTATATACACAGTTGGTTCAGTTCAACTGGAATGACAAGTGGGCTGTATTTTAACAACGGGTGGCTCGGTTTTGAACAAACCATGCATACTGCACGTTTTCTCTGTTTTGAACAAACCATGCATACTGCACGTTTTCTCTGTTTTTATCAAATAAAGTTTGCAGATAGGTAGAATCAATATGTTCTTGCTAGTATTTTATTTTTGATATCCATGAGTGTTCGGGTCAGCTTGCGTACACCTCAACTAATTTCACGAAACAACCTTGCTAGTATTTTTTATTAAAGAAGTTCTAATTTGCTTGTGAAACAGTTGGATGAAGTTGACATGGGAATGACATATGAGGAGCTCTCAGTCTATGGAAGGCTGCGAAAAATTTTCCGTTGTGGTCCCGTGTCGATGTTTAAGGTTTGTCAAATATTTTCTTACTGCTTTTCTGGTGTAGCCTGAGAGGATAAAAGAAGAATTCTCTCCAAGAATCAATCAAATCTTTATTACGCATTAAAAGTTAGGAATACAATAGGACAATTCTCAAATTATATAGAGAATTGGAAAATAAGCTAATTCTAATCCTAATTAGGACAAGGAAACTAATCCTAATCCTAATTAACCAGGGAACTAATCTCAATCCCAATTAATCAAAGAAACTAATTCTAATCCTAATTAATCAAGTATTTAACCAAGATACCCTAATTTACCCTAATCCCACCATATCATTTTTTTTGCTTGACAATCTCATAAACTTCTAACTCTTCATCCTTGTCACAGAATCTTTGCTACCGTTGGGGTTCTAAGTTAACTCCATCAGAGGTGGCCGAAAAAGTGAAACATTTCTTCAAGTATTACTCCATCAATCGACACAAAATGACCGTACTAACGCCATCTTATCACGCCGAGGTAGGTATCTTCGATTGACTGAAATTGATGGTAAAAAGATATCAGGATGTCATGATCTACACGTCTATGTTTCTCATTCTTTTCTTATCGAAATTGCCTTGCAGAGCTATTCTCCGGAGGACAATCGGTTTGATCTTCGTCAATTTCTCTACAACTCGAGATGGCCATATCAGTTTCGGAAGATCGATGATCTTGTAAAAGAGTTGAATGGAGATAGCATTGCTATTAAAGAATCAACTGGTATGGGGGTTGTGGCAGCAGGCGCAGGGAAACCCAATGTTGGACTTTAG

mRNA sequence

ATGAGGTTATTGAAAGTGGCAACCTGTAATTTGAATCAATGGGCTATGGATTTCGATTGTAATGTGAAGCACATCAAAGAGTCCATTGACGAGGCTAAACGTGCTGGGGCTGTCATTAGACTTGGTCCGGAACTCGAGATTACTGGATATGGCTGTGAAGACCATTTCTTGGAGCTCGACACTGTCGCTCATGCATGGGAATGCTTGAAGGATATACTGTTGGGTCCTTGGACTGATGGGATATTGTGCAGCATTGGCATGCCTGTTATTAAAGACTCTGAGCGTTACAATTGTCAAGTGCTATGCTATAATAGGAAAATTATTATGATACGCCCAAAGATGTGGCTTGCAAATGATGGGAATTACCGGGAGCTCCGCTGGTTCACAGCATGGAAGCTAAAGGACAAACTTGTGGACTTTCAGCTGCCAAAAGATATTTCTGAGGCTCTGTCACAAGCGTCTGCGCCTTTTGGCTATGGATATATTCAGTTTCTAGACACAGCTGTTGCTGCTGAAGTTTGTGAAGAACTATTTACGCCTACTCCTCCTCATGCTGAACTTGCTTTGAATGGGGTCGAAGTATTTATGAACGCTAGTGGAAGTCATCACCAACTAAGAAAACTTGACGTTCGTCTTCGTGCTTTTACAGGTGCTACTCATAATCGTGGTGGAGTTTACATGTATAGTAACCACCAGGGTTGTGACGGTGGTCGCCTTTACTATGATGGATGTGCCTGTGTTGTTATAAACGGTGATCTGGTAGCTCAAGGCTCACAGTTTTCTTTGAAGGATGTTGAGGTTGTGGTTGCCCATGTAGATCTTGATGCGGTTGCTAGTCTTAGAGGATCTATCAGCAGCTTTCAAGAACAAGCAAGCTATAAAACTAAAGTTCCTTCTGTGGAAGCTCCATACAGTCTATGTCAACCATTCAACCTCAAAACTTCCCTTTCAAGTCCACTTGAGATTAACTATCACTGTGCGGAAGAGGAAATAGCCTTTGGACCTGGTTGCTGGTTATGGGACTATTTAAGACGAAGTGGAGCTTCTGGATTTCTGCTTCCTCTTTCTGGTGGAGCTGATAGCTCCTCTGTTGCAGCCATAGTTGGCTGCATGTGCCAGCTTGTCGTAAAAGATATTGCAAATGGAGACGAACAGGTGAAAGCTGATGCAATACGGATTGGACATTATGCTGATGGAGAGTTCCCAACTGATAGCAGAGAATTTGCCAGACGCATTTTTTATACTGTATTTATGGGTTCTGAAAATAGCTCTGAAGCAACACGTTCAAGAGCAAAGGTTCTAGCAGATGAGATTGGTTCATGGCATCTTGATGTTTCCATCGATGGGATTATTTCAGCACTTTTGTCTTTGTTCCAAACTCTCACTGGAAAACGTCCACGGTATAAGGTAGATGGGGGATCTAATATTGAAAATTTAGGTCTGCAAAATATTCAAGCTCGAATCAGGATGGTTCTGGCATTTATGTTAGCGTCACTCTTACCTTGGGTACATAGTAAACCCGGATTCTACCTTGTTCTTGGTAGCTCCAATGTCGATGAAGGATTGCGTGGTTACTTAACAAAGTATGATTGCAGTTCAGCAGATATAAATCCAATTGGAAGCATCAGTAAGCAGGATCTCCGGGCATTTCTTAGATGGGCTGCCACCCATCTTGGCTACTCATCGTTGGCGGATATCGAAGCAGCTCCGCCTACAGCTGAGCTGGAGCCAATTCGTTCCAACTACAGCCAGTTGGATGAAGTTGACATGGGAATGACATATGAGGAGCTCTCAGTCTATGGAAGGCTGCGAAAAATTTTCCGTTGTGGTCCCGTGTCGATGTTTAAGAATCTTTGCTACCGTTGGGGTTCTAAGTTAACTCCATCAGAGGTGGCCGAAAAAGTGAAACATTTCTTCAAGTATTACTCCATCAATCGACACAAAATGACCGTACTAACGCCATCTTATCACGCCGAGAGCTATTCTCCGGAGGACAATCGGTTTGATCTTCGTCAATTTCTCTACAACTCGAGATGGCCATATCAGTTTCGGAAGATCGATGATCTTGTAAAAGAGTTGAATGGAGATAGCATTGCTATTAAAGAATCAACTGGTATGGGGGTTGTGGCAGCAGGCGCAGGGAAACCCAATGTTGGACTTTAG

Coding sequence (CDS)

ATGAGGTTATTGAAAGTGGCAACCTGTAATTTGAATCAATGGGCTATGGATTTCGATTGTAATGTGAAGCACATCAAAGAGTCCATTGACGAGGCTAAACGTGCTGGGGCTGTCATTAGACTTGGTCCGGAACTCGAGATTACTGGATATGGCTGTGAAGACCATTTCTTGGAGCTCGACACTGTCGCTCATGCATGGGAATGCTTGAAGGATATACTGTTGGGTCCTTGGACTGATGGGATATTGTGCAGCATTGGCATGCCTGTTATTAAAGACTCTGAGCGTTACAATTGTCAAGTGCTATGCTATAATAGGAAAATTATTATGATACGCCCAAAGATGTGGCTTGCAAATGATGGGAATTACCGGGAGCTCCGCTGGTTCACAGCATGGAAGCTAAAGGACAAACTTGTGGACTTTCAGCTGCCAAAAGATATTTCTGAGGCTCTGTCACAAGCGTCTGCGCCTTTTGGCTATGGATATATTCAGTTTCTAGACACAGCTGTTGCTGCTGAAGTTTGTGAAGAACTATTTACGCCTACTCCTCCTCATGCTGAACTTGCTTTGAATGGGGTCGAAGTATTTATGAACGCTAGTGGAAGTCATCACCAACTAAGAAAACTTGACGTTCGTCTTCGTGCTTTTACAGGTGCTACTCATAATCGTGGTGGAGTTTACATGTATAGTAACCACCAGGGTTGTGACGGTGGTCGCCTTTACTATGATGGATGTGCCTGTGTTGTTATAAACGGTGATCTGGTAGCTCAAGGCTCACAGTTTTCTTTGAAGGATGTTGAGGTTGTGGTTGCCCATGTAGATCTTGATGCGGTTGCTAGTCTTAGAGGATCTATCAGCAGCTTTCAAGAACAAGCAAGCTATAAAACTAAAGTTCCTTCTGTGGAAGCTCCATACAGTCTATGTCAACCATTCAACCTCAAAACTTCCCTTTCAAGTCCACTTGAGATTAACTATCACTGTGCGGAAGAGGAAATAGCCTTTGGACCTGGTTGCTGGTTATGGGACTATTTAAGACGAAGTGGAGCTTCTGGATTTCTGCTTCCTCTTTCTGGTGGAGCTGATAGCTCCTCTGTTGCAGCCATAGTTGGCTGCATGTGCCAGCTTGTCGTAAAAGATATTGCAAATGGAGACGAACAGGTGAAAGCTGATGCAATACGGATTGGACATTATGCTGATGGAGAGTTCCCAACTGATAGCAGAGAATTTGCCAGACGCATTTTTTATACTGTATTTATGGGTTCTGAAAATAGCTCTGAAGCAACACGTTCAAGAGCAAAGGTTCTAGCAGATGAGATTGGTTCATGGCATCTTGATGTTTCCATCGATGGGATTATTTCAGCACTTTTGTCTTTGTTCCAAACTCTCACTGGAAAACGTCCACGGTATAAGGTAGATGGGGGATCTAATATTGAAAATTTAGGTCTGCAAAATATTCAAGCTCGAATCAGGATGGTTCTGGCATTTATGTTAGCGTCACTCTTACCTTGGGTACATAGTAAACCCGGATTCTACCTTGTTCTTGGTAGCTCCAATGTCGATGAAGGATTGCGTGGTTACTTAACAAAGTATGATTGCAGTTCAGCAGATATAAATCCAATTGGAAGCATCAGTAAGCAGGATCTCCGGGCATTTCTTAGATGGGCTGCCACCCATCTTGGCTACTCATCGTTGGCGGATATCGAAGCAGCTCCGCCTACAGCTGAGCTGGAGCCAATTCGTTCCAACTACAGCCAGTTGGATGAAGTTGACATGGGAATGACATATGAGGAGCTCTCAGTCTATGGAAGGCTGCGAAAAATTTTCCGTTGTGGTCCCGTGTCGATGTTTAAGAATCTTTGCTACCGTTGGGGTTCTAAGTTAACTCCATCAGAGGTGGCCGAAAAAGTGAAACATTTCTTCAAGTATTACTCCATCAATCGACACAAAATGACCGTACTAACGCCATCTTATCACGCCGAGAGCTATTCTCCGGAGGACAATCGGTTTGATCTTCGTCAATTTCTCTACAACTCGAGATGGCCATATCAGTTTCGGAAGATCGATGATCTTGTAAAAGAGTTGAATGGAGATAGCATTGCTATTAAAGAATCAACTGGTATGGGGGTTGTGGCAGCAGGCGCAGGGAAACCCAATGTTGGACTTTAG

Protein sequence

MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELDTVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDGNYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTPTPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLYYDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSVEAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGADSSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGSENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLGLQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIGSISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVYGRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAESYSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAIKESTGMGVVAAGAGKPNVGL
Homology
BLAST of Lag0032618 vs. NCBI nr
Match: XP_022145998.1 (glutamine-dependent NAD(+) synthetase [Momordica charantia])

HSP 1 Score: 1439.1 bits (3724), Expect = 0.0e+00
Identity = 698/720 (96.94%), Postives = 706/720 (98.06%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWKLKDKLVDFQLP+DISEALSQ S PFGYGYIQFLDTAVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTAVAAEVCEELFTP 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLDVRLRAF GATH RGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKT VPSV
Sbjct: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTTVPSV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
            APYSLCQ FNLK SLSSPLEINYHCAEEEIA+GPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQSFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSEATRSRAKVLADEIGSWHLDVSIDGI+SALLSLFQTLTGKRPRYKVDGGSNIENLG
Sbjct: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFM ASLLPWVHSK GFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHSKSGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GRLRKIFRCGPVSMFKNLCYRWG+KLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGTKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAIKESTGMGVVAAGAGKPNVGL 720
           YSPEDNRFDLRQFLYNSRWPYQFRKID+LVKELNGDSIAIKES GMGVVAAG+G P+VGL
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDNLVKELNGDSIAIKESDGMGVVAAGSGNPSVGL 720

BLAST of Lag0032618 vs. NCBI nr
Match: XP_038875301.1 (glutamine-dependent NAD(+) synthetase isoform X1 [Benincasa hispida])

HSP 1 Score: 1427.2 bits (3693), Expect = 0.0e+00
Identity = 690/720 (95.83%), Postives = 703/720 (97.64%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWKLKDKLVDFQLPKDISEALSQ S PFGYGYIQFLD AVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQTSVPFGYGYIQFLDAAVAAEVCEELFTP 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLDVRLRAF GATH RGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGCACVV+NGDLVAQGSQFSLKDVEV+VAHVDLDAVASLRGSISSFQEQASYKTKVPSV
Sbjct: 241 YDGCACVVVNGDLVAQGSQFSLKDVEVIVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
            APYSLCQ F+LK SLSSPLEI YHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQSFDLKISLSSPLEIKYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IANGDEQVKADAIRIGHYADGEFPTDSREFA+RIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAIRIGHYADGEFPTDSREFAKRIFYTVFMGS 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSEATR+RAKVLA EIGSWHLDVSIDGI+SA LSLFQTLTGKRP+YKVDGGSNIENLG
Sbjct: 421 ENSSEATRTRAKVLAHEIGSWHLDVSIDGIVSAFLSLFQTLTGKRPQYKVDGGSNIENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFM ASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISKQDLRAFLRWA+THL YSSLADIEAAPPTAELEPIRS+YSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKQDLRAFLRWASTHLCYSSLADIEAAPPTAELEPIRSDYSQLDEVDMGMTYEELSVY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GRLRKIFRCGPVSMFKNLCYRWG+KLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGAKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAIKESTGMGVVAAGAGKPNVGL 720
           YSPEDNRFDLRQFLYNSRWPYQFRKID LVKELNGDSIAIKES+GMGVVAAG+G PNVGL
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDKLVKELNGDSIAIKESSGMGVVAAGSGNPNVGL 720

BLAST of Lag0032618 vs. NCBI nr
Match: XP_004143863.1 (glutamine-dependent NAD(+) synthetase isoform X1 [Cucumis sativus])

HSP 1 Score: 1419.8 bits (3674), Expect = 0.0e+00
Identity = 684/720 (95.00%), Postives = 699/720 (97.08%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEI+GYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEISGYGCEDHFLELD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQ++CYNRKIIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQIMCYNRKIIMIRPKMWLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWKLKDKLVDFQLPKD++EALSQ S PFGYGYIQF DTAVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPKDVAEALSQTSVPFGYGYIQFQDTAVAAEVCEELFTP 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLDVRLRAF GATH RGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGCACVV+NGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV
Sbjct: 241 YDGCACVVVNGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
            APYSLCQ FNLK SLSSPLEI YHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQSFNLKISLSSPLEIKYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IANGDEQVKADAIRIGHYADGE PTDSREFARRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAIRIGHYADGELPTDSREFARRIFYTVFMGS 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSE TR+RAKVLA EIGSWHLDVSIDGI+SALLSLFQTLTGKRPRYKVDGGSNIENLG
Sbjct: 421 ENSSEETRTRAKVLAHEIGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFM ASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISK DLRAFLRWA+THL YSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKMDLRAFLRWASTHLSYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GR+RKIFRCGPVSMFKNLCYRWG+KLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES
Sbjct: 601 GRMRKIFRCGPVSMFKNLCYRWGAKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAIKESTGMGVVAAGAGKPNVGL 720
           YSPEDNRFDLRQFLYNSRWPYQFRKID LV+ELNGD IAIKES+GMGVVAAG+G P VGL
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDKLVEELNGDGIAIKESSGMGVVAAGSGNPKVGL 720

BLAST of Lag0032618 vs. NCBI nr
Match: KAG6579559.1 (Glutamine-dependent NAD(+) synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1416.0 bits (3664), Expect = 0.0e+00
Identity = 684/721 (94.87%), Postives = 703/721 (97.50%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDFDCNVKHIKESI+EAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 22  MRLLKVATCNLNQWAMDFDCNVKHIKESIEEAKRAGAVIRLGPELEITGYGCEDHFLELD 81

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLKDILLG WTDGILCS GMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG
Sbjct: 82  TVNHAWECLKDILLGHWTDGILCSFGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 141

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWKLKDKLVDFQLP+DISEALSQ S PFGYGYIQFLDTAVAAEVCEELFTP
Sbjct: 142 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTAVAAEVCEELFTP 201

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLDVRLRAF GATH RGGVYMYSNHQGCDGGRLY
Sbjct: 202 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 261

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGC+CVV+NGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV
Sbjct: 262 YDGCSCVVVNGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 321

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
            A YSLCQ FNLK SLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 322 AASYSLCQSFNLKISLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 381

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IANGDEQVKADA+RIGHYA+G+FPTDSREFARRIFYTVFMGS
Sbjct: 382 SSSVAAIVGCMCQLVVKEIANGDEQVKADAVRIGHYAEGQFPTDSREFARRIFYTVFMGS 441

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSEATR+RAKVLADE+GSWHLDVSIDGI+SALLSLFQTLTGKRPRYKVDGGSNIENLG
Sbjct: 442 ENSSEATRTRAKVLADEVGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 501

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 502 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 561

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISKQDLRAFLRWAATHL YSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 562 SISKQDLRAFLRWAATHLRYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 621

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GRLRKI+RCGPVSMFKNLCYRWG+KL+PSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES
Sbjct: 622 GRLRKIYRCGPVSMFKNLCYRWGAKLSPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 681

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAI-KESTGMGVVAAGAGKPNVG 720
           YSPEDNRFDLRQFLYNSRWPYQF KID LVKELNGDS+ + +ES+GMGVVAAG+G PNVG
Sbjct: 682 YSPEDNRFDLRQFLYNSRWPYQFGKIDKLVKELNGDSVGVRRESSGMGVVAAGSGNPNVG 741

BLAST of Lag0032618 vs. NCBI nr
Match: XP_023549734.1 (glutamine-dependent NAD(+) synthetase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1416.0 bits (3664), Expect = 0.0e+00
Identity = 684/721 (94.87%), Postives = 703/721 (97.50%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDFDCNVKHIKESI+EAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIEEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLKDILLG WTDGILCS GMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG
Sbjct: 61  TVNHAWECLKDILLGHWTDGILCSFGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWKLKDKLVDFQLP+DISEALSQ S PFGYGYIQFLDTAVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTAVAAEVCEELFTP 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLD+RLRAF GATH RGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDLRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGC+CVV+NGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV
Sbjct: 241 YDGCSCVVVNGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
            A YSLCQ FNLK SLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AASYSLCQSFNLKISLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IANGDEQVKADA+RIGHYA+GEFPTDSREFARRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAVRIGHYAEGEFPTDSREFARRIFYTVFMGS 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSEATR+RAKVLADE+GSWHLDVSIDGI+SALLSLFQTLTGKRPRYKVDGGSNIENLG
Sbjct: 421 ENSSEATRTRAKVLADEVGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISKQDLRAFLRWAATHL YSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKQDLRAFLRWAATHLRYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GRLRKI+RCGPVSMFKNLCYRWG+KL+PSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES
Sbjct: 601 GRLRKIYRCGPVSMFKNLCYRWGAKLSPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAI-KESTGMGVVAAGAGKPNVG 720
           YSPEDNRFDLRQFLYNSRWPYQF KID LVKELNGDS+ + +ES+GMGVVAAG+G PNVG
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFGKIDKLVKELNGDSVGVRRESSGMGVVAAGSGNPNVG 720

BLAST of Lag0032618 vs. ExPASy Swiss-Prot
Match: Q9C723 (Glutamine-dependent NAD(+) synthetase OS=Arabidopsis thaliana OX=3702 GN=At1g55090 PE=2 SV=1)

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 603/725 (83.17%), Postives = 663/725 (91.45%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDF+ N+K+IK SI EAK AGAVIRLGPELE+TGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFESNMKNIKASIAEAKAAGAVIRLGPELEVTGYGCEDHFLELD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLK++LLG WTD ILCSIGMPVIK +ERYNCQVLC NR+IIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKELLLGDWTDDILCSIGMPVIKGAERYNCQVLCMNRRIIMIRPKMWLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWK +++L +FQLP +ISEAL Q S PFGYGYIQF+DTAVAAEVCEELF+P
Sbjct: 121 NYRELRWFTAWKQREELEEFQLPIEISEALEQKSVPFGYGYIQFIDTAVAAEVCEELFSP 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLD+RL AF GATH RGGVYMYSN QGCDG RLY
Sbjct: 181 LPPHAELALNGVEVFMNASGSHHQLRKLDIRLNAFMGATHARGGVYMYSNQQGCDGSRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGCAC+V+NG++VAQGSQFSL+DVEV+++ VDLDAVASLRGSISSFQEQAS K KV SV
Sbjct: 241 YDGCACIVVNGNVVAQGSQFSLRDVEVIISQVDLDAVASLRGSISSFQEQASCKVKVSSV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
             P  L Q FNLK +LSSP +I YH  +EEIAFGP CW+WDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AVPCRLTQSFNLKMTLSSPKKIIYHSPQEEIAFGPACWMWDYLRRSGASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IA GDEQVKADA RIG+YA+G+FPTDS+EFA+RIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIAKGDEQVKADANRIGNYANGQFPTDSKEFAKRIFYTVFMGS 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSE T+ R+K LADEIG+WHLDV IDG++SA+LSLFQT+TGKRPRYKVDGGSN ENLG
Sbjct: 421 ENSSEETKRRSKQLADEIGAWHLDVCIDGVVSAVLSLFQTVTGKRPRYKVDGGSNAENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQAR+RMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARMRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISK DLR FL+WAAT+LGY SLA+IEAAPPTAELEPIRS+YSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKMDLRLFLKWAATNLGYPSLAEIEAAPPTAELEPIRSDYSQLDEVDMGMTYEELSVY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GR+RKIFRCGPVSMFKNLCY+WG+KL+P+EVAEKVK+FFKYYSINRHKMTVLTPSYHAES
Sbjct: 601 GRMRKIFRCGPVSMFKNLCYKWGTKLSPAEVAEKVKYFFKYYSINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAIKESTG-----MGVVAAGAGK 720
           YSPEDNRFDLRQFLYNS+WPYQF+KID++V  LNGDS+A  E        +GVVAA +G 
Sbjct: 661 YSPEDNRFDLRQFLYNSKWPYQFKKIDEIVDSLNGDSVAFPEEEANSNKEIGVVAANSGD 720

BLAST of Lag0032618 vs. ExPASy Swiss-Prot
Match: A2YII8 (Glutamine-dependent NAD(+) synthetase OS=Oryza sativa subsp. indica OX=39946 GN=OsI_25032 PE=3 SV=1)

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 559/734 (76.16%), Postives = 630/734 (85.83%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLL+VATCNLNQWAMDFD N++++KESI  AK AGA +R+GPELE+TGYGCEDHFLE D
Sbjct: 1   MRLLRVATCNLNQWAMDFDTNLRNVKESIARAKAAGAAVRVGPELELTGYGCEDHFLEQD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           T AHAWECLKDIL G +TDGILCSIGMPVI  S RYNCQV C N KI+MIRPK+ LANDG
Sbjct: 61  TAAHAWECLKDILSGGYTDGILCSIGMPVIFKSVRYNCQVFCLNSKIVMIRPKISLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRE RWF+AW  KD LVDFQLP DISE  SQ + PFGYG+IQFLD ++A+E CEELFT 
Sbjct: 121 NYREFRWFSAWTFKDALVDFQLPLDISEVTSQDTVPFGYGFIQFLDVSLASETCEELFTA 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
             P  +LALNGVEVF+NASGSHHQLRKL +R+ +   AT   GGVYMY+N QGCDGGRLY
Sbjct: 181 NAPRIDLALNGVEVFVNASGSHHQLRKLSLRIDSMRNATLACGGVYMYANQQGCDGGRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGC C+ +NGD+VAQGSQFSLKDVEV+ A VDLDAV+S R S+SSF+EQAS++TKVP V
Sbjct: 241 YDGCCCIAVNGDVVAQGSQFSLKDVEVLDALVDLDAVSSYRASVSSFREQASHRTKVPFV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
           + PY LC+PF      + P+E+ YH  EEEIAFGP CWLWDYLRRS ASGFLLPLSGGAD
Sbjct: 301 KVPYKLCKPFQSGMVPTGPVEVMYHRPEEEIAFGPSCWLWDYLRRSRASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVKDI NGDEQVKADA+RIG Y DGEFP DSRE A+R+FYTV+MG+
Sbjct: 361 SSSVAAIVGCMCQLVVKDIENGDEQVKADAMRIGQYKDGEFPKDSRELAKRLFYTVYMGT 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSE TRSRAK+LA+EIGS+HLDV ID I+SALLSLF+ LTGKRPRYKVDGGSN ENLG
Sbjct: 421 ENSSEGTRSRAKMLAEEIGSFHLDVPIDSIVSALLSLFERLTGKRPRYKVDGGSNTENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFM+ASL+PWVH+K GFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARIRMVLAFMMASLMPWVHNKSGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           S+SKQDLRAFLRWAA HL YSSLA++EAAPPTAELEPIR++Y+QLDEVDMGMTYEELS+Y
Sbjct: 541 SVSKQDLRAFLRWAAVHLHYSSLAEVEAAPPTAELEPIRADYNQLDEVDMGMTYEELSIY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GRLRKIFRCGPVSMF+NLC+RW   L+PSEVA+KVKHFFKYY+INRHKMTVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFQNLCHRWCGTLSPSEVADKVKHFFKYYAINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGD---------------SIAIKESTG 720
           YSPEDNRFDLRQFLYN+RWPYQFRKID+LV++++ D                +   E  G
Sbjct: 661 YSPEDNRFDLRQFLYNARWPYQFRKIDELVQDMDKDGKWVNSTEGELRRRKGVRSAEGGG 720

BLAST of Lag0032618 vs. ExPASy Swiss-Prot
Match: Q0D8D4 (Glutamine-dependent NAD(+) synthetase OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0167100 PE=3 SV=2)

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 559/734 (76.16%), Postives = 630/734 (85.83%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLL+VATCNLNQWAMDFD N++++KESI  AK AGA +R+GPELE+TGYGCEDHFLE D
Sbjct: 1   MRLLRVATCNLNQWAMDFDTNLRNVKESIARAKAAGAAVRVGPELELTGYGCEDHFLEQD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           T AHAWECLKDIL G +TDGILCSIGMPVI  S RYNCQV C N KI+MIRPK+ LANDG
Sbjct: 61  TAAHAWECLKDILSGGYTDGILCSIGMPVIFKSVRYNCQVFCLNSKIVMIRPKISLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRE RWF+AW  KD LVDFQLP DISE  SQ + PFGYG+IQFLD ++A+E CEELFT 
Sbjct: 121 NYREFRWFSAWTFKDALVDFQLPLDISEVTSQDTVPFGYGFIQFLDVSLASETCEELFTA 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
             P  +LALNGVEVF+NASGSHHQLRKL +R+ +   AT   GGVYMY+N QGCDGGRLY
Sbjct: 181 NAPRIDLALNGVEVFVNASGSHHQLRKLSLRIDSMRNATLACGGVYMYANQQGCDGGRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGC C+ +NGD+VAQGSQFSLKDVEV+ A VDLDAV+S R S+SSF+EQAS++TKVP V
Sbjct: 241 YDGCCCIAVNGDVVAQGSQFSLKDVEVLDALVDLDAVSSYRASVSSFREQASHRTKVPFV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
           + PY LC+PF      + P+E+ YH  EEEIAFGP CWLWDYLRRS ASGFLLPLSGGAD
Sbjct: 301 KVPYKLCKPFQSGMVPTGPVEVMYHRPEEEIAFGPSCWLWDYLRRSRASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVKDI NGDEQVKADA+RIG Y DGEFP DSRE A+R+FYTV+MG+
Sbjct: 361 SSSVAAIVGCMCQLVVKDIENGDEQVKADAMRIGQYKDGEFPKDSRELAKRLFYTVYMGT 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSE TRSRAK+LA+EIGS+HLDV ID I+SALLSLF+ LTGKRPRYKVDGGSN ENLG
Sbjct: 421 ENSSEGTRSRAKMLAEEIGSFHLDVPIDSIVSALLSLFERLTGKRPRYKVDGGSNTENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFM+ASL+PWVH+K GFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARIRMVLAFMMASLMPWVHNKSGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           S+SKQDLRAFLRWAA HL YSSLA++EAAPPTAELEPIR++Y+QLDEVDMGMTYEELS+Y
Sbjct: 541 SVSKQDLRAFLRWAAVHLHYSSLAEVEAAPPTAELEPIRADYNQLDEVDMGMTYEELSIY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GRLRKIFRCGPVSMF+NLC+RW   L+PSEVA+KVKHFFKYY+INRHKMTVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFQNLCHRWCGTLSPSEVADKVKHFFKYYAINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGD---------------SIAIKESTG 720
           YSPEDNRFDLRQFLYN+RWPYQFRKID+LV++++ D                +   E  G
Sbjct: 661 YSPEDNRFDLRQFLYNARWPYQFRKIDELVQDMDKDGKWVNSTEGELRRRKGVRSAEGGG 720

BLAST of Lag0032618 vs. ExPASy Swiss-Prot
Match: Q54ML1 (Glutamine-dependent NAD(+) synthetase OS=Dictyostelium discoideum OX=44689 GN=nadsyn1 PE=3 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 1.8e-229
Identity = 395/701 (56.35%), Postives = 492/701 (70.19%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           M+ + +ATCNLNQWAMDF  N++ I ESI+ AK  GA  RLGPELEI GYGCEDHFLE D
Sbjct: 1   MKTVTLATCNLNQWAMDFKGNLERIIESINIAKSKGAKYRLGPELEICGYGCEDHFLEQD 60

Query: 61  TVAHAWECLKDILLGP-WTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLAND 120
           T+ H W+ L  IL  P  T  IL  +GMPV+    RYNC+V+  N+KI +I+PK  +A D
Sbjct: 61  TMLHCWQSLAVILKDPELTKDILVDVGMPVLHKDVRYNCRVILLNQKIYLIQPKKAMAMD 120

Query: 121 GNYRELRWFTAWKLKDKLVD-FQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELF 180
           GNYRE RWFT W +K ++V+ F LP+ IS+   Q     G   I  LDTA+++E CEELF
Sbjct: 121 GNYREGRWFTPW-IKPRVVETFYLPRIISQITGQDECQIGDAIISTLDTAISSETCEELF 180

Query: 181 TPTPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGR 240
           TP  PH ++ L+GVE+F N SGSHHQLRKLD R+     AT   GG+Y+YSN QGCDG R
Sbjct: 181 TPNSPHIQMGLDGVEIFTNGSGSHHQLRKLDTRVDLIRSATSKSGGIYLYSNQQGCDGSR 240

Query: 241 LYYDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVP 300
           LYYDG   ++INGD V+QGSQFSL D+EV+ A VDL+ V S+R S  +   QA+   + P
Sbjct: 241 LYYDGSCMIMINGDCVSQGSQFSLVDIEVITATVDLEDVRSVRASFMARCAQANLTKEFP 300

Query: 301 SVEAPYSL-----CQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLL 360
            V  P  L     C P          + INY+   EEI FGP CWLWDYLRRSG SG+ L
Sbjct: 301 RVRCPIQLTHIDYCHP------PDRVIHINYNTPAEEIGFGPACWLWDYLRRSGLSGYFL 360

Query: 361 PLSGGADSSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIF 420
           PLSGGADS++ AAI+G MCQLV+ D++ G++QV  DA RI +  +   PTDSREFA R+F
Sbjct: 361 PLSGGADSAATAAIIGIMCQLVILDVSKGNKQVLKDAQRITNSPEDYIPTDSREFASRLF 420

Query: 421 YTVFMGSENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGG 480
           +T ++GS+NSS+ TR RA  +A +IGS H +V ID I  +    F  +T K+P+++  GG
Sbjct: 421 FTAYLGSKNSSKETRDRAMEIAKDIGSVHKEVDIDDISQSFNDAFSQITKKQPQFRAHGG 480

Query: 481 SNIENLGLQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSS 540
           +  ENL LQN+QAR RMVL++ LASLL W   +PG  LVLGS+N DE LRGY+TKYDCSS
Sbjct: 481 TPRENLALQNVQARTRMVLSYHLASLLLWEQGRPGSLLVLGSANCDESLRGYMTKYDCSS 540

Query: 541 ADINPIGSISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMT 600
           ADINPIG +SK DLR+F+ WA       S+  +  A PTAELEPI  NY+Q DE+DMGMT
Sbjct: 541 ADINPIGGMSKIDLRSFIEWAGKFRDMKSILSVLTATPTAELEPITENYTQSDEIDMGMT 600

Query: 601 YEELSVYGRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLT 660
           YEELS++G+LRK+ RCGPVSMF+ L   W + L PS VAEKVK FF YY+INRHK+T LT
Sbjct: 601 YEELSIFGKLRKVNRCGPVSMFERLVADW-AHLEPSVVAEKVKRFFYYYAINRHKLTTLT 660

Query: 661 PSYHAESYSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELN 695
           PSYHAE YSP+DNR+D RQFLYNS+W  QF  ID +V  L+
Sbjct: 661 PSYHAEGYSPDDNRYDHRQFLYNSKWDVQFETIDKIVLRLS 693

BLAST of Lag0032618 vs. ExPASy Swiss-Prot
Match: P38795 (Glutamine-dependent NAD(+) synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=QNS1 PE=1 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 3.0e-224
Identity = 385/699 (55.08%), Postives = 485/699 (69.38%), Query Frame = 0

Query: 3   LLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELDTV 62
           L+ +ATCNLNQWA+DF+ N   I +SI  AK  GA +R+GPELEITGYGC DHFLE D  
Sbjct: 4   LITLATCNLNQWALDFEGNRDRILQSIKIAKERGARLRVGPELEITGYGCLDHFLENDVC 63

Query: 63  AHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDGNY 122
            H+WE    I+    T G++  IGMPV+  + RYNC++L  + +I+ IRPK+WLANDGNY
Sbjct: 64  LHSWEMYAQIIKNKETHGLILDIGMPVLHKNVRYNCRLLSLDGEILFIRPKIWLANDGNY 123

Query: 123 RELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTPTP 182
           RE+R+FT W     + DF LP +I +   Q   PFG   I  LDT +  E CEELFTP  
Sbjct: 124 REMRFFTPWMKPGVVEDFILPPEIQKVTGQRLVPFGDAVINSLDTCIGTETCEELFTPQS 183

Query: 183 PHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLYYD 242
           PH  ++L+GVE+  N+SGSHH+LRKL+ RL     AT   GGVY+Y+N +GCDG RLYYD
Sbjct: 184 PHIAMSLDGVEIMTNSSGSHHELRKLNKRLDLILNATKRCGGVYLYANQRGCDGDRLYYD 243

Query: 243 GCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASY-KTKVPSVE 302
           GCA + ING +VAQGSQFSL DVEVV A VDL+ V S R ++ S   QAS  + K   ++
Sbjct: 244 GCALIAINGTIVAQGSQFSLDDVEVVTATVDLEEVRSYRAAVMSRGLQASLAEIKFKRID 303

Query: 303 APYSLC------QPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPL 362
            P  L        P    T +  P    YH  EEEIA GP CW+WDYLRR   +GF LPL
Sbjct: 304 IPVELALMTSRFDPTVCPTKVREPF---YHSPEEEIALGPACWMWDYLRRCNGTGFFLPL 363

Query: 363 SGGADSSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYT 422
           SGG DS + A IV  MC+LV     NG+EQV  D  +I    D   P   ++ A +IF++
Sbjct: 364 SGGIDSCATAMIVHSMCRLVTDAAQNGNEQVIKDVRKITRSGDDWIPDSPQDLASKIFHS 423

Query: 423 VFMGSENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSN 482
            FMG+ENSS+ TR+RAK L++ IGS+H+D+ +D ++S+++SLF+  TGK+P YK+ GGS 
Sbjct: 424 CFMGTENSSKETRNRAKDLSNAIGSYHVDLKMDSLVSSVVSLFEVATGKKPIYKIFGGSQ 483

Query: 483 IENLGLQNIQARIRMVLAFMLASLLPWVHSKP--GFYLVLGSSNVDEGLRGYLTKYDCSS 542
           IENL LQNIQAR+RMVL+++ A LLPWV   P  G  LVLGS+NVDE LRGYLTKYDCSS
Sbjct: 484 IENLALQNIQARLRMVLSYLFAQLLPWVRGIPNSGGLLVLGSANVDECLRGYLTKYDCSS 543

Query: 543 ADINPIGSISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMT 602
           ADINPIG ISK DL+ F+ +A+       L D   A PTAELEP+  +Y Q DE+DMGMT
Sbjct: 544 ADINPIGGISKTDLKRFIAYASKQYNMPILNDFLNATPTAELEPMTKDYVQSDEIDMGMT 603

Query: 603 YEELSVYGRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLT 662
           YEEL V+G LRK+ +CGP SMF  L ++W  KLTP +++EKVK FF +Y+INRHK TVLT
Sbjct: 604 YEELGVFGYLRKVEKCGPYSMFLKLLHQWSPKLTPRQISEKVKRFFFFYAINRHKQTVLT 663

Query: 663 PSYHAESYSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKE 693
           PSYHAE YSPEDNRFDLR FL N R+P+  RKID++V++
Sbjct: 664 PSYHAEQYSPEDNRFDLRPFLINPRFPWASRKIDEVVEQ 699

BLAST of Lag0032618 vs. ExPASy TrEMBL
Match: A0A6J1CXE6 (Glutamine-dependent NAD(+) synthetase OS=Momordica charantia OX=3673 GN=LOC111015310 PE=3 SV=1)

HSP 1 Score: 1439.1 bits (3724), Expect = 0.0e+00
Identity = 698/720 (96.94%), Postives = 706/720 (98.06%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWKLKDKLVDFQLP+DISEALSQ S PFGYGYIQFLDTAVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTAVAAEVCEELFTP 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLDVRLRAF GATH RGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKT VPSV
Sbjct: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTTVPSV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
            APYSLCQ FNLK SLSSPLEINYHCAEEEIA+GPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQSFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSEATRSRAKVLADEIGSWHLDVSIDGI+SALLSLFQTLTGKRPRYKVDGGSNIENLG
Sbjct: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFM ASLLPWVHSK GFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHSKSGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GRLRKIFRCGPVSMFKNLCYRWG+KLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGTKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAIKESTGMGVVAAGAGKPNVGL 720
           YSPEDNRFDLRQFLYNSRWPYQFRKID+LVKELNGDSIAIKES GMGVVAAG+G P+VGL
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDNLVKELNGDSIAIKESDGMGVVAAGSGNPSVGL 720

BLAST of Lag0032618 vs. ExPASy TrEMBL
Match: A0A0A0KMF0 (Glutamine-dependent NAD(+) synthetase OS=Cucumis sativus OX=3659 GN=Csa_5G149340 PE=3 SV=1)

HSP 1 Score: 1419.8 bits (3674), Expect = 0.0e+00
Identity = 684/720 (95.00%), Postives = 699/720 (97.08%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEI+GYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEISGYGCEDHFLELD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQ++CYNRKIIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQIMCYNRKIIMIRPKMWLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWKLKDKLVDFQLPKD++EALSQ S PFGYGYIQF DTAVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPKDVAEALSQTSVPFGYGYIQFQDTAVAAEVCEELFTP 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLDVRLRAF GATH RGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGCACVV+NGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV
Sbjct: 241 YDGCACVVVNGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
            APYSLCQ FNLK SLSSPLEI YHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQSFNLKISLSSPLEIKYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IANGDEQVKADAIRIGHYADGE PTDSREFARRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAIRIGHYADGELPTDSREFARRIFYTVFMGS 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSE TR+RAKVLA EIGSWHLDVSIDGI+SALLSLFQTLTGKRPRYKVDGGSNIENLG
Sbjct: 421 ENSSEETRTRAKVLAHEIGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFM ASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISK DLRAFLRWA+THL YSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKMDLRAFLRWASTHLSYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GR+RKIFRCGPVSMFKNLCYRWG+KLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES
Sbjct: 601 GRMRKIFRCGPVSMFKNLCYRWGAKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAIKESTGMGVVAAGAGKPNVGL 720
           YSPEDNRFDLRQFLYNSRWPYQFRKID LV+ELNGD IAIKES+GMGVVAAG+G P VGL
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDKLVEELNGDGIAIKESSGMGVVAAGSGNPKVGL 720

BLAST of Lag0032618 vs. ExPASy TrEMBL
Match: A0A6J1EKV5 (Glutamine-dependent NAD(+) synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435595 PE=3 SV=1)

HSP 1 Score: 1416.0 bits (3664), Expect = 0.0e+00
Identity = 684/721 (94.87%), Postives = 703/721 (97.50%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDFDCNVKHIKESI+EAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIEEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLKDILLG WTDGILCS GMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG
Sbjct: 61  TVNHAWECLKDILLGHWTDGILCSFGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWKLKDKLVDFQLP+DISEALSQ S PFGYGYIQFLDTAVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTAVAAEVCEELFTP 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLDVRLRAF GATH RGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGC+CVV+NGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV
Sbjct: 241 YDGCSCVVVNGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
            A YSLCQ FNLK SLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AASYSLCQSFNLKISLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IANGDEQVKADA+RIGHYA+GE PTDSREFARRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAVRIGHYAEGELPTDSREFARRIFYTVFMGS 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSEATR+RAKVLADE+GSWHLDVSIDGI+SALLSLFQTLTGKRPRYKVDGGSNIENLG
Sbjct: 421 ENSSEATRTRAKVLADEVGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISKQDLRAFLRWAATHL YSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKQDLRAFLRWAATHLRYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GRLRKI+RCGPVSMFKNLCYRWG+KL+PSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES
Sbjct: 601 GRLRKIYRCGPVSMFKNLCYRWGAKLSPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAI-KESTGMGVVAAGAGKPNVG 720
           YSPEDNRFDLRQFLYNSRWPYQF KID+LVKELNGDS+ + +ES+GMGVVAAG+G PNVG
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFGKIDELVKELNGDSVGVRRESSGMGVVAAGSGNPNVG 720

BLAST of Lag0032618 vs. ExPASy TrEMBL
Match: A0A1S3ATK9 (Glutamine-dependent NAD(+) synthetase OS=Cucumis melo OX=3656 GN=LOC103482827 PE=3 SV=1)

HSP 1 Score: 1414.1 bits (3659), Expect = 0.0e+00
Identity = 683/720 (94.86%), Postives = 696/720 (96.67%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWKLKDKLVDFQLPKD++EAL Q S PFGYGYIQF DTAVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPKDVAEALLQTSVPFGYGYIQFQDTAVAAEVCEELFTP 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLDVRLRAF GATH RGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGCACVV+NGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKT VPSV
Sbjct: 241 YDGCACVVVNGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTTVPSV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
             PYSLC+ FNLK SLSSP EI YHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AVPYSLCESFNLKISLSSPREIKYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSE TR+RAKVLA EIGSWHLDVSIDGI+SALLSLFQTLTGKRPRYKVDGGSNIENLG
Sbjct: 421 ENSSEETRTRAKVLAHEIGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFM ASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISK DLRAFLRWA+THL YSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKMDLRAFLRWASTHLSYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GRLRKIFRCGPVSMFKNLCYRWG+KLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGAKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAIKESTGMGVVAAGAGKPNVGL 720
           YSPEDNRFDLRQFLYNSRWPYQFRKID LV+EL+GD IAIKES+GMGVVAAG+G P VGL
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDKLVEELDGDGIAIKESSGMGVVAAGSGNPKVGL 720

BLAST of Lag0032618 vs. ExPASy TrEMBL
Match: A0A5A7TLS0 (Glutamine-dependent NAD(+) synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001810 PE=3 SV=1)

HSP 1 Score: 1412.9 bits (3656), Expect = 0.0e+00
Identity = 682/720 (94.72%), Postives = 696/720 (96.67%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWKLKDKLVDFQLPKD++EAL Q S PFGYGYIQF DTAVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPKDVAEALLQTSVPFGYGYIQFQDTAVAAEVCEELFTP 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLDVRLRAF GATH RGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGCACVV+NGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKT VPS+
Sbjct: 241 YDGCACVVVNGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTTVPSL 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
             PYSLC+ FNLK SLSSP EI YHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AVPYSLCESFNLKISLSSPREIKYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSE TR+RAKVLA EIGSWHLDVSIDGI+SALLSLFQTLTGKRPRYKVDGGSNIENLG
Sbjct: 421 ENSSEETRTRAKVLAHEIGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQARIRMVLAFM ASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISK DLRAFLRWA+THL YSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKMDLRAFLRWASTHLSYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GRLRKIFRCGPVSMFKNLCYRWG+KLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGAKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAIKESTGMGVVAAGAGKPNVGL 720
           YSPEDNRFDLRQFLYNSRWPYQFRKID LV+EL+GD IAIKES+GMGVVAAG+G P VGL
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDKLVEELDGDGIAIKESSGMGVVAAGSGNPKVGL 720

BLAST of Lag0032618 vs. TAIR 10
Match: AT1G55090.1 (carbon-nitrogen hydrolase family protein )

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 603/725 (83.17%), Postives = 663/725 (91.45%), Query Frame = 0

Query: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60
           MRLLKVATCNLNQWAMDF+ N+K+IK SI EAK AGAVIRLGPELE+TGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFESNMKNIKASIAEAKAAGAVIRLGPELEVTGYGCEDHFLELD 60

Query: 61  TVAHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120
           TV HAWECLK++LLG WTD ILCSIGMPVIK +ERYNCQVLC NR+IIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKELLLGDWTDDILCSIGMPVIKGAERYNCQVLCMNRRIIMIRPKMWLANDG 120

Query: 121 NYRELRWFTAWKLKDKLVDFQLPKDISEALSQASAPFGYGYIQFLDTAVAAEVCEELFTP 180
           NYRELRWFTAWK +++L +FQLP +ISEAL Q S PFGYGYIQF+DTAVAAEVCEELF+P
Sbjct: 121 NYRELRWFTAWKQREELEEFQLPIEISEALEQKSVPFGYGYIQFIDTAVAAEVCEELFSP 180

Query: 181 TPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFTGATHNRGGVYMYSNHQGCDGGRLY 240
            PPHAELALNGVEVFMNASGSHHQLRKLD+RL AF GATH RGGVYMYSN QGCDG RLY
Sbjct: 181 LPPHAELALNGVEVFMNASGSHHQLRKLDIRLNAFMGATHARGGVYMYSNQQGCDGSRLY 240

Query: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300
           YDGCAC+V+NG++VAQGSQFSL+DVEV+++ VDLDAVASLRGSISSFQEQAS K KV SV
Sbjct: 241 YDGCACIVVNGNVVAQGSQFSLRDVEVIISQVDLDAVASLRGSISSFQEQASCKVKVSSV 300

Query: 301 EAPYSLCQPFNLKTSLSSPLEINYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360
             P  L Q FNLK +LSSP +I YH  +EEIAFGP CW+WDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AVPCRLTQSFNLKMTLSSPKKIIYHSPQEEIAFGPACWMWDYLRRSGASGFLLPLSGGAD 360

Query: 361 SSSVAAIVGCMCQLVVKDIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420
           SSSVAAIVGCMCQLVVK+IA GDEQVKADA RIG+YA+G+FPTDS+EFA+RIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIAKGDEQVKADANRIGNYANGQFPTDSKEFAKRIFYTVFMGS 420

Query: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIISALLSLFQTLTGKRPRYKVDGGSNIENLG 480
           ENSSE T+ R+K LADEIG+WHLDV IDG++SA+LSLFQT+TGKRPRYKVDGGSN ENLG
Sbjct: 421 ENSSEETKRRSKQLADEIGAWHLDVCIDGVVSAVLSLFQTVTGKRPRYKVDGGSNAENLG 480

Query: 481 LQNIQARIRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540
           LQNIQAR+RMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG
Sbjct: 481 LQNIQARMRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600
           SISK DLR FL+WAAT+LGY SLA+IEAAPPTAELEPIRS+YSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKMDLRLFLKWAATNLGYPSLAEIEAAPPTAELEPIRSDYSQLDEVDMGMTYEELSVY 600

Query: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660
           GR+RKIFRCGPVSMFKNLCY+WG+KL+P+EVAEKVK+FFKYYSINRHKMTVLTPSYHAES
Sbjct: 601 GRMRKIFRCGPVSMFKNLCYKWGTKLSPAEVAEKVKYFFKYYSINRHKMTVLTPSYHAES 660

Query: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDDLVKELNGDSIAIKESTG-----MGVVAAGAGK 720
           YSPEDNRFDLRQFLYNS+WPYQF+KID++V  LNGDS+A  E        +GVVAA +G 
Sbjct: 661 YSPEDNRFDLRQFLYNSKWPYQFKKIDEIVDSLNGDSVAFPEEEANSNKEIGVVAANSGD 720

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145998.10.0e+0096.94glutamine-dependent NAD(+) synthetase [Momordica charantia][more]
XP_038875301.10.0e+0095.83glutamine-dependent NAD(+) synthetase isoform X1 [Benincasa hispida][more]
XP_004143863.10.0e+0095.00glutamine-dependent NAD(+) synthetase isoform X1 [Cucumis sativus][more]
KAG6579559.10.0e+0094.87Glutamine-dependent NAD(+) synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023549734.10.0e+0094.87glutamine-dependent NAD(+) synthetase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9C7230.0e+0083.17Glutamine-dependent NAD(+) synthetase OS=Arabidopsis thaliana OX=3702 GN=At1g550... [more]
A2YII80.0e+0076.16Glutamine-dependent NAD(+) synthetase OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q0D8D40.0e+0076.16Glutamine-dependent NAD(+) synthetase OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q54ML11.8e-22956.35Glutamine-dependent NAD(+) synthetase OS=Dictyostelium discoideum OX=44689 GN=na... [more]
P387953.0e-22455.08Glutamine-dependent NAD(+) synthetase OS=Saccharomyces cerevisiae (strain ATCC 2... [more]
Match NameE-valueIdentityDescription
A0A6J1CXE60.0e+0096.94Glutamine-dependent NAD(+) synthetase OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A0A0KMF00.0e+0095.00Glutamine-dependent NAD(+) synthetase OS=Cucumis sativus OX=3659 GN=Csa_5G149340... [more]
A0A6J1EKV50.0e+0094.87Glutamine-dependent NAD(+) synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435... [more]
A0A1S3ATK90.0e+0094.86Glutamine-dependent NAD(+) synthetase OS=Cucumis melo OX=3656 GN=LOC103482827 PE... [more]
A0A5A7TLS00.0e+0094.72Glutamine-dependent NAD(+) synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
Match NameE-valueIdentityDescription
AT1G55090.10.0e+0083.17carbon-nitrogen hydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022310NAD/GMP synthasePFAMPF02540NAD_synthasecoord: 338..598
e-value: 2.2E-25
score: 89.2
IPR014445Glutamine-dependent NAD(+) synthetasePIRSFPIRSF006630NADS_GATcoord: 1..682
e-value: 2.4E-152
score: 506.2
IPR014445Glutamine-dependent NAD(+) synthetaseHAMAPMF_02090NadE_glutamine_depcoord: 4..680
score: 28.490139
IPR003010Carbon-nitrogen hydrolasePFAMPF00795CN_hydrolasecoord: 5..281
e-value: 3.4E-29
score: 102.0
IPR003010Carbon-nitrogen hydrolasePROSITEPS50263CN_HYDROLASEcoord: 4..274
score: 43.32851
IPR036526Carbon-nitrogen hydrolase superfamilyGENE3D3.60.110.10coord: 1..305
e-value: 8.5E-69
score: 233.9
IPR036526Carbon-nitrogen hydrolase superfamilySUPERFAMILY56317Carbon-nitrogen hydrolasecoord: 1..289
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 327..694
e-value: 2.7E-124
score: 416.7
IPR003694NAD(+) synthetasePANTHERPTHR23090NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASEcoord: 1..720
IPR003694NAD(+) synthetaseCDDcd00553NAD_synthasecoord: 328..649
e-value: 4.52321E-90
score: 279.818
NoneNo IPR availablePANTHERPTHR23090:SF16GLUTAMINE-DEPENDENT NAD(+) SYNTHETASEcoord: 1..720
NoneNo IPR availableCDDcd07570GAT_Gln-NAD-synthcoord: 5..292
e-value: 5.20571E-89
score: 277.814
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 317..661

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032618.1Lag0032618.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009435 NAD biosynthetic process
biological_process GO:0006807 nitrogen compound metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004359 glutaminase activity
molecular_function GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity