Lag0032616 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0032616 vs. NCBI nr
Match: XP_022146068.1 (nuclear intron maturase 4, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 1423.3 bits (3683), Expect = 0.0e+00
Identity = 702/792 (88.64%), Postives = 744/792 (93.94%), Query Frame = 0

Query: 1   MNMQNFAVLRSVHCKVNSSFVSDIGKCVQRVQSSENYSALACVDDEIDKGMEKKKLAINL 60
           MNM+NFAVLR   CKVNSSFVSDIGKCVQRVQ+SENYSALAC DD+  KGMEKKKLA NL
Sbjct: 20  MNMRNFAVLRI--CKVNSSFVSDIGKCVQRVQTSENYSALACADDDFCKGMEKKKLAENL 79

Query: 61  ASLVEESLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNAY 120
           ASLVEESL VD +RPK+RMEL+RSLEIQ+KKRVKAQY+NGKFMDLMGKVIACP TLQNAY
Sbjct: 80  ASLVEESLDVDSRRPKSRMELKRSLEIQIKKRVKAQYVNGKFMDLMGKVIACPPTLQNAY 139

Query: 121 DCIRLNSNVDIASNDHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVLQ 180
           DC+R+NSNVDIASNDHL+SF+SMAEEL NGSFDVN NTFSISSS+KEVLILPKLKLKVLQ
Sbjct: 140 DCVRINSNVDIASNDHLISFESMAEELHNGSFDVNANTFSISSSKKEVLILPKLKLKVLQ 199

Query: 181 EAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVMA 240
           EAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEI+NPDWWFT+D+SKKMDEL MA
Sbjct: 200 EAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEINNPDWWFTVDISKKMDELEMA 259

Query: 241 KIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLFD 300
           K+I+VMEDKIEDP  FA+IRSIF+AGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLFD
Sbjct: 260 KLISVMEDKIEDPEFFAIIRSIFEAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLFD 319

Query: 301 QEFFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYMD 360
           QEFFRLSMKYEAINKYGNA +D SQSKLRSWFRR LKGNDSEYP +EKDNIRVYCCRYMD
Sbjct: 320 QEFFRLSMKYEAINKYGNAVQDGSQSKLRSWFRRKLKGNDSEYPAQEKDNIRVYCCRYMD 379

Query: 361 EIFLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMKE 420
           EIF+AVSGSKDV +SFRSEI +FI KSLHLD+NHQ EMVSC ETRG+RFLGCLVRRS KE
Sbjct: 380 EIFMAVSGSKDVALSFRSEIQDFIQKSLHLDVNHQEEMVSCRETRGIRFLGCLVRRSEKE 439

Query: 421 SPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSLN 480
           SPAVKAVHKLKEKVELFALQKQEAWN WTVWLGKKWLAHGLKKVKESEIKHLAKNSPSLN
Sbjct: 440 SPAVKAVHKLKEKVELFALQKQEAWNDWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSLN 499

Query: 481 QISSFRKAGMETDHWYKVLLKIWMQDINAKAAESEETILSNHVVQPSLPLELRDSFHEFQ 540
           QISSFRK GMETDHWYKVLLKIWMQDINAKAAE+EETILSN+VV+PSLPLELRDSF+EFQ
Sbjct: 500 QISSFRKVGMETDHWYKVLLKIWMQDINAKAAETEETILSNYVVEPSLPLELRDSFYEFQ 559

Query: 541 RCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKRLFRYRLITNKGYPCPSP 600
           R VE+YVSSETAST+ALLPNYDPSVKSTFITEIIAPV+SIRKRL RYRLITNKGYPC SP
Sbjct: 560 RRVEEYVSSETASTVALLPNYDPSVKSTFITEIIAPVNSIRKRLLRYRLITNKGYPCASP 619

Query: 601 FLILQDNNQIIDWFSGVSRRWLQWYSICSNFSELILICDQVRKSCIRTLAAKHRIHESEI 660
           FLIL DN QIIDWF GV RRWL+WYS CSNFSE+ILICDQVRKSCIRTLAAKHR HESEI
Sbjct: 620 FLILHDNTQIIDWFLGVYRRWLKWYSNCSNFSEVILICDQVRKSCIRTLAAKHRTHESEI 679

Query: 661 EKKFDSELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGISYSGLCLLSLARMVSQSRP 720
           EKKFD ELSRI S+PE+EQEEE+  SDTH L HDEA  YGISYSGLCLLSLARMVSQSRP
Sbjct: 680 EKKFDLELSRICSTPEIEQEEEEEASDTHGLGHDEASTYGISYSGLCLLSLARMVSQSRP 739

Query: 721 CNCFVIGCLVPAPSVYTLHVMERQKFPGWNTGFSSSIHPSLNRRRFGLCKQHLKDLYLGH 780
           CNCFV+GCL  APSVYTLHVMERQKFPGW TGFSSSIHPSLNRRR GLCKQHLKDLYLGH
Sbjct: 740 CNCFVMGCLASAPSVYTLHVMERQKFPGWKTGFSSSIHPSLNRRRVGLCKQHLKDLYLGH 799

Query: 781 ISLQSIDFGAWK 793
           ISLQS++FGAWK
Sbjct: 800 ISLQSVNFGAWK 809

BLAST of Lag0032616 vs. NCBI nr
Match: XP_022146067.1 (nuclear intron maturase 4, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 1418.7 bits (3671), Expect = 0.0e+00
Identity = 702/793 (88.52%), Postives = 744/793 (93.82%), Query Frame = 0

Query: 1   MNMQNFAVLRSVHCKVNSSFVSDI-GKCVQRVQSSENYSALACVDDEIDKGMEKKKLAIN 60
           MNM+NFAVLR   CKVNSSFVSDI GKCVQRVQ+SENYSALAC DD+  KGMEKKKLA N
Sbjct: 20  MNMRNFAVLRI--CKVNSSFVSDIAGKCVQRVQTSENYSALACADDDFCKGMEKKKLAEN 79

Query: 61  LASLVEESLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNA 120
           LASLVEESL VD +RPK+RMEL+RSLEIQ+KKRVKAQY+NGKFMDLMGKVIACP TLQNA
Sbjct: 80  LASLVEESLDVDSRRPKSRMELKRSLEIQIKKRVKAQYVNGKFMDLMGKVIACPPTLQNA 139

Query: 121 YDCIRLNSNVDIASNDHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVL 180
           YDC+R+NSNVDIASNDHL+SF+SMAEEL NGSFDVN NTFSISSS+KEVLILPKLKLKVL
Sbjct: 140 YDCVRINSNVDIASNDHLISFESMAEELHNGSFDVNANTFSISSSKKEVLILPKLKLKVL 199

Query: 181 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVM 240
           QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEI+NPDWWFT+D+SKKMDEL M
Sbjct: 200 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEINNPDWWFTVDISKKMDELEM 259

Query: 241 AKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF 300
           AK+I+VMEDKIEDP  FA+IRSIF+AGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF
Sbjct: 260 AKLISVMEDKIEDPEFFAIIRSIFEAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF 319

Query: 301 DQEFFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYM 360
           DQEFFRLSMKYEAINKYGNA +D SQSKLRSWFRR LKGNDSEYP +EKDNIRVYCCRYM
Sbjct: 320 DQEFFRLSMKYEAINKYGNAVQDGSQSKLRSWFRRKLKGNDSEYPAQEKDNIRVYCCRYM 379

Query: 361 DEIFLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMK 420
           DEIF+AVSGSKDV +SFRSEI +FI KSLHLD+NHQ EMVSC ETRG+RFLGCLVRRS K
Sbjct: 380 DEIFMAVSGSKDVALSFRSEIQDFIQKSLHLDVNHQEEMVSCRETRGIRFLGCLVRRSEK 439

Query: 421 ESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL 480
           ESPAVKAVHKLKEKVELFALQKQEAWN WTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL
Sbjct: 440 ESPAVKAVHKLKEKVELFALQKQEAWNDWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL 499

Query: 481 NQISSFRKAGMETDHWYKVLLKIWMQDINAKAAESEETILSNHVVQPSLPLELRDSFHEF 540
           NQISSFRK GMETDHWYKVLLKIWMQDINAKAAE+EETILSN+VV+PSLPLELRDSF+EF
Sbjct: 500 NQISSFRKVGMETDHWYKVLLKIWMQDINAKAAETEETILSNYVVEPSLPLELRDSFYEF 559

Query: 541 QRCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKRLFRYRLITNKGYPCPS 600
           QR VE+YVSSETAST+ALLPNYDPSVKSTFITEIIAPV+SIRKRL RYRLITNKGYPC S
Sbjct: 560 QRRVEEYVSSETASTVALLPNYDPSVKSTFITEIIAPVNSIRKRLLRYRLITNKGYPCAS 619

Query: 601 PFLILQDNNQIIDWFSGVSRRWLQWYSICSNFSELILICDQVRKSCIRTLAAKHRIHESE 660
           PFLIL DN QIIDWF GV RRWL+WYS CSNFSE+ILICDQVRKSCIRTLAAKHR HESE
Sbjct: 620 PFLILHDNTQIIDWFLGVYRRWLKWYSNCSNFSEVILICDQVRKSCIRTLAAKHRTHESE 679

Query: 661 IEKKFDSELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGISYSGLCLLSLARMVSQSR 720
           IEKKFD ELSRI S+PE+EQEEE+  SDTH L HDEA  YGISYSGLCLLSLARMVSQSR
Sbjct: 680 IEKKFDLELSRICSTPEIEQEEEEEASDTHGLGHDEASTYGISYSGLCLLSLARMVSQSR 739

Query: 721 PCNCFVIGCLVPAPSVYTLHVMERQKFPGWNTGFSSSIHPSLNRRRFGLCKQHLKDLYLG 780
           PCNCFV+GCL  APSVYTLHVMERQKFPGW TGFSSSIHPSLNRRR GLCKQHLKDLYLG
Sbjct: 740 PCNCFVMGCLASAPSVYTLHVMERQKFPGWKTGFSSSIHPSLNRRRVGLCKQHLKDLYLG 799

Query: 781 HISLQSIDFGAWK 793
           HISLQS++FGAWK
Sbjct: 800 HISLQSVNFGAWK 810

BLAST of Lag0032616 vs. NCBI nr
Match: XP_022146069.1 (nuclear intron maturase 4, mitochondrial isoform X3 [Momordica charantia])

HSP 1 Score: 1418.7 bits (3671), Expect = 0.0e+00
Identity = 702/793 (88.52%), Postives = 744/793 (93.82%), Query Frame = 0

Query: 1   MNMQNFAVLRSVHCKVNSSFVSDI-GKCVQRVQSSENYSALACVDDEIDKGMEKKKLAIN 60
           MNM+NFAVLR   CKVNSSFVSDI GKCVQRVQ+SENYSALAC DD+  KGMEKKKLA N
Sbjct: 12  MNMRNFAVLRI--CKVNSSFVSDIAGKCVQRVQTSENYSALACADDDFCKGMEKKKLAEN 71

Query: 61  LASLVEESLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNA 120
           LASLVEESL VD +RPK+RMEL+RSLEIQ+KKRVKAQY+NGKFMDLMGKVIACP TLQNA
Sbjct: 72  LASLVEESLDVDSRRPKSRMELKRSLEIQIKKRVKAQYVNGKFMDLMGKVIACPPTLQNA 131

Query: 121 YDCIRLNSNVDIASNDHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVL 180
           YDC+R+NSNVDIASNDHL+SF+SMAEEL NGSFDVN NTFSISSS+KEVLILPKLKLKVL
Sbjct: 132 YDCVRINSNVDIASNDHLISFESMAEELHNGSFDVNANTFSISSSKKEVLILPKLKLKVL 191

Query: 181 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVM 240
           QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEI+NPDWWFT+D+SKKMDEL M
Sbjct: 192 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEINNPDWWFTVDISKKMDELEM 251

Query: 241 AKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF 300
           AK+I+VMEDKIEDP  FA+IRSIF+AGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF
Sbjct: 252 AKLISVMEDKIEDPEFFAIIRSIFEAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF 311

Query: 301 DQEFFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYM 360
           DQEFFRLSMKYEAINKYGNA +D SQSKLRSWFRR LKGNDSEYP +EKDNIRVYCCRYM
Sbjct: 312 DQEFFRLSMKYEAINKYGNAVQDGSQSKLRSWFRRKLKGNDSEYPAQEKDNIRVYCCRYM 371

Query: 361 DEIFLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMK 420
           DEIF+AVSGSKDV +SFRSEI +FI KSLHLD+NHQ EMVSC ETRG+RFLGCLVRRS K
Sbjct: 372 DEIFMAVSGSKDVALSFRSEIQDFIQKSLHLDVNHQEEMVSCRETRGIRFLGCLVRRSEK 431

Query: 421 ESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL 480
           ESPAVKAVHKLKEKVELFALQKQEAWN WTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL
Sbjct: 432 ESPAVKAVHKLKEKVELFALQKQEAWNDWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL 491

Query: 481 NQISSFRKAGMETDHWYKVLLKIWMQDINAKAAESEETILSNHVVQPSLPLELRDSFHEF 540
           NQISSFRK GMETDHWYKVLLKIWMQDINAKAAE+EETILSN+VV+PSLPLELRDSF+EF
Sbjct: 492 NQISSFRKVGMETDHWYKVLLKIWMQDINAKAAETEETILSNYVVEPSLPLELRDSFYEF 551

Query: 541 QRCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKRLFRYRLITNKGYPCPS 600
           QR VE+YVSSETAST+ALLPNYDPSVKSTFITEIIAPV+SIRKRL RYRLITNKGYPC S
Sbjct: 552 QRRVEEYVSSETASTVALLPNYDPSVKSTFITEIIAPVNSIRKRLLRYRLITNKGYPCAS 611

Query: 601 PFLILQDNNQIIDWFSGVSRRWLQWYSICSNFSELILICDQVRKSCIRTLAAKHRIHESE 660
           PFLIL DN QIIDWF GV RRWL+WYS CSNFSE+ILICDQVRKSCIRTLAAKHR HESE
Sbjct: 612 PFLILHDNTQIIDWFLGVYRRWLKWYSNCSNFSEVILICDQVRKSCIRTLAAKHRTHESE 671

Query: 661 IEKKFDSELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGISYSGLCLLSLARMVSQSR 720
           IEKKFD ELSRI S+PE+EQEEE+  SDTH L HDEA  YGISYSGLCLLSLARMVSQSR
Sbjct: 672 IEKKFDLELSRICSTPEIEQEEEEEASDTHGLGHDEASTYGISYSGLCLLSLARMVSQSR 731

Query: 721 PCNCFVIGCLVPAPSVYTLHVMERQKFPGWNTGFSSSIHPSLNRRRFGLCKQHLKDLYLG 780
           PCNCFV+GCL  APSVYTLHVMERQKFPGW TGFSSSIHPSLNRRR GLCKQHLKDLYLG
Sbjct: 732 PCNCFVMGCLASAPSVYTLHVMERQKFPGWKTGFSSSIHPSLNRRRVGLCKQHLKDLYLG 791

Query: 781 HISLQSIDFGAWK 793
           HISLQS++FGAWK
Sbjct: 792 HISLQSVNFGAWK 802

BLAST of Lag0032616 vs. NCBI nr
Match: XP_038882003.1 (nuclear intron maturase 4, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 1383.6 bits (3580), Expect = 0.0e+00
Identity = 678/793 (85.50%), Postives = 738/793 (93.06%), Query Frame = 0

Query: 1   MNMQNFAVLRSVH-CKVNSSFVSDIGKCVQRVQSSENYSALACVDDEIDKGMEKKKLAIN 60
           +NM+N   L  V+ CKV+SS VS IGK VQRVQ+SENYS L C DDEIDKGMEK KLA+N
Sbjct: 46  INMRNLTNLLCVNVCKVDSSVVSVIGKSVQRVQNSENYSTLTCADDEIDKGMEKMKLAMN 105

Query: 61  LASLVEESLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNA 120
           LASLVEESL VDLKR KT+MEL+RSLEIQ+K+RVKAQY+NGKF+DLMGKVIACPTTLQNA
Sbjct: 106 LASLVEESLDVDLKRSKTQMELKRSLEIQIKERVKAQYLNGKFLDLMGKVIACPTTLQNA 165

Query: 121 YDCIRLNSNVDIASNDHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVL 180
           YDC+R+NSNVDI SND L+SF+SMAEEL NG+FDVN NTFSI SSRKEVL+LPK++LKVL
Sbjct: 166 YDCVRINSNVDIMSNDCLISFESMAEELSNGNFDVNANTFSILSSRKEVLVLPKIELKVL 225

Query: 181 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVM 240
           QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEI NPDWWFT+DLSKKMDELVM
Sbjct: 226 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIKNPDWWFTIDLSKKMDELVM 285

Query: 241 AKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF 300
           AK+ITVMEDKIEDP LFAVIRSI+ AGALNLEFGGFPKGHGLPQEG+LSPIL NIYLNLF
Sbjct: 286 AKLITVMEDKIEDPKLFAVIRSIYVAGALNLEFGGFPKGHGLPQEGILSPILTNIYLNLF 345

Query: 301 DQEFFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYM 360
           DQEFFRLSMKYEAIN+YGN G+D SQS+LRSWFRR LKGN S+YPGE+KD IRVYCCRYM
Sbjct: 346 DQEFFRLSMKYEAINEYGNTGQDGSQSRLRSWFRRQLKGNSSDYPGEQKDKIRVYCCRYM 405

Query: 361 DEIFLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMK 420
           DEIFLAVSGSKDV +SFRSEI  F+ K+LHLD+NHQ EMVSC ET G+RFLGCLVRRS++
Sbjct: 406 DEIFLAVSGSKDVALSFRSEIFYFLQKTLHLDVNHQEEMVSCGETHGIRFLGCLVRRSVQ 465

Query: 421 ESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL 480
           ESPAVK+VHKLK+KVELFALQKQE WNAWTVWLGKKWLAHGLKKVKESEIKHLAKNS SL
Sbjct: 466 ESPAVKSVHKLKKKVELFALQKQETWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNS-SL 525

Query: 481 NQISSFRKAGMETDHWYKVLLKIWMQDINAKAAESEETILSNHVVQPSLPLELRDSFHEF 540
           NQISSFRKAGMETDHWYKVLLKIWMQD+NA+AAESEE ILS H V+PSLPLELRDSF+EF
Sbjct: 526 NQISSFRKAGMETDHWYKVLLKIWMQDLNARAAESEEKILSKHAVEPSLPLELRDSFYEF 585

Query: 541 QRCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKRLFRYRLITNKGYPCPS 600
           QRCV++Y+S+ETAST+ALLPNYDPSVK TFITEIIAPV+SIRKRL RYRL+TNKG+PC S
Sbjct: 586 QRCVQEYISAETASTVALLPNYDPSVKPTFITEIIAPVNSIRKRLLRYRLVTNKGHPCSS 645

Query: 601 PFLILQDNNQIIDWFSGVSRRWLQWYSICSNFSELILICDQVRKSCIRTLAAKHRIHESE 660
           PFLILQDN QIIDWF GVSRRW +WY+ CSNFSELILICD VRKSCIRTLAAKHRIHESE
Sbjct: 646 PFLILQDNTQIIDWFLGVSRRWFRWYNNCSNFSELILICDLVRKSCIRTLAAKHRIHESE 705

Query: 661 IEKKFDSELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGISYSGLCLLSLARMVSQSR 720
           IEKKFDSELS++YSSPE+EQEEEK + DTH LDHDEAL YGISYSGLCLLSLARMVSQSR
Sbjct: 706 IEKKFDSELSKMYSSPEIEQEEEK-SPDTHGLDHDEALKYGISYSGLCLLSLARMVSQSR 765

Query: 721 PCNCFVIGCLVPAPSVYTLHVMERQKFPGWNTGFSSSIHPSLNRRRFGLCKQHLKDLYLG 780
           PCNCFV+GCL PAPSVYTLHVMERQKFPGW TGFSSSIHPSLN+RRFGLCK+HL+DLYLG
Sbjct: 766 PCNCFVVGCLAPAPSVYTLHVMERQKFPGWKTGFSSSIHPSLNKRRFGLCKKHLEDLYLG 825

Query: 781 HISLQSIDFGAWK 793
           HISLQSIDFGAWK
Sbjct: 826 HISLQSIDFGAWK 836

BLAST of Lag0032616 vs. NCBI nr
Match: XP_038882001.1 (nuclear intron maturase 4, mitochondrial isoform X1 [Benincasa hispida] >XP_038882002.1 nuclear intron maturase 4, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 1383.6 bits (3580), Expect = 0.0e+00
Identity = 678/793 (85.50%), Postives = 738/793 (93.06%), Query Frame = 0

Query: 1   MNMQNFAVLRSVH-CKVNSSFVSDIGKCVQRVQSSENYSALACVDDEIDKGMEKKKLAIN 60
           +NM+N   L  V+ CKV+SS VS IGK VQRVQ+SENYS L C DDEIDKGMEK KLA+N
Sbjct: 92  INMRNLTNLLCVNVCKVDSSVVSVIGKSVQRVQNSENYSTLTCADDEIDKGMEKMKLAMN 151

Query: 61  LASLVEESLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNA 120
           LASLVEESL VDLKR KT+MEL+RSLEIQ+K+RVKAQY+NGKF+DLMGKVIACPTTLQNA
Sbjct: 152 LASLVEESLDVDLKRSKTQMELKRSLEIQIKERVKAQYLNGKFLDLMGKVIACPTTLQNA 211

Query: 121 YDCIRLNSNVDIASNDHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVL 180
           YDC+R+NSNVDI SND L+SF+SMAEEL NG+FDVN NTFSI SSRKEVL+LPK++LKVL
Sbjct: 212 YDCVRINSNVDIMSNDCLISFESMAEELSNGNFDVNANTFSILSSRKEVLVLPKIELKVL 271

Query: 181 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVM 240
           QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEI NPDWWFT+DLSKKMDELVM
Sbjct: 272 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIKNPDWWFTIDLSKKMDELVM 331

Query: 241 AKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF 300
           AK+ITVMEDKIEDP LFAVIRSI+ AGALNLEFGGFPKGHGLPQEG+LSPIL NIYLNLF
Sbjct: 332 AKLITVMEDKIEDPKLFAVIRSIYVAGALNLEFGGFPKGHGLPQEGILSPILTNIYLNLF 391

Query: 301 DQEFFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYM 360
           DQEFFRLSMKYEAIN+YGN G+D SQS+LRSWFRR LKGN S+YPGE+KD IRVYCCRYM
Sbjct: 392 DQEFFRLSMKYEAINEYGNTGQDGSQSRLRSWFRRQLKGNSSDYPGEQKDKIRVYCCRYM 451

Query: 361 DEIFLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMK 420
           DEIFLAVSGSKDV +SFRSEI  F+ K+LHLD+NHQ EMVSC ET G+RFLGCLVRRS++
Sbjct: 452 DEIFLAVSGSKDVALSFRSEIFYFLQKTLHLDVNHQEEMVSCGETHGIRFLGCLVRRSVQ 511

Query: 421 ESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL 480
           ESPAVK+VHKLK+KVELFALQKQE WNAWTVWLGKKWLAHGLKKVKESEIKHLAKNS SL
Sbjct: 512 ESPAVKSVHKLKKKVELFALQKQETWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNS-SL 571

Query: 481 NQISSFRKAGMETDHWYKVLLKIWMQDINAKAAESEETILSNHVVQPSLPLELRDSFHEF 540
           NQISSFRKAGMETDHWYKVLLKIWMQD+NA+AAESEE ILS H V+PSLPLELRDSF+EF
Sbjct: 572 NQISSFRKAGMETDHWYKVLLKIWMQDLNARAAESEEKILSKHAVEPSLPLELRDSFYEF 631

Query: 541 QRCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKRLFRYRLITNKGYPCPS 600
           QRCV++Y+S+ETAST+ALLPNYDPSVK TFITEIIAPV+SIRKRL RYRL+TNKG+PC S
Sbjct: 632 QRCVQEYISAETASTVALLPNYDPSVKPTFITEIIAPVNSIRKRLLRYRLVTNKGHPCSS 691

Query: 601 PFLILQDNNQIIDWFSGVSRRWLQWYSICSNFSELILICDQVRKSCIRTLAAKHRIHESE 660
           PFLILQDN QIIDWF GVSRRW +WY+ CSNFSELILICD VRKSCIRTLAAKHRIHESE
Sbjct: 692 PFLILQDNTQIIDWFLGVSRRWFRWYNNCSNFSELILICDLVRKSCIRTLAAKHRIHESE 751

Query: 661 IEKKFDSELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGISYSGLCLLSLARMVSQSR 720
           IEKKFDSELS++YSSPE+EQEEEK + DTH LDHDEAL YGISYSGLCLLSLARMVSQSR
Sbjct: 752 IEKKFDSELSKMYSSPEIEQEEEK-SPDTHGLDHDEALKYGISYSGLCLLSLARMVSQSR 811

Query: 721 PCNCFVIGCLVPAPSVYTLHVMERQKFPGWNTGFSSSIHPSLNRRRFGLCKQHLKDLYLG 780
           PCNCFV+GCL PAPSVYTLHVMERQKFPGW TGFSSSIHPSLN+RRFGLCK+HL+DLYLG
Sbjct: 812 PCNCFVVGCLAPAPSVYTLHVMERQKFPGWKTGFSSSIHPSLNKRRFGLCKKHLEDLYLG 871

Query: 781 HISLQSIDFGAWK 793
           HISLQSIDFGAWK
Sbjct: 872 HISLQSIDFGAWK 882

BLAST of Lag0032616 vs. ExPASy Swiss-Prot
Match: Q9CA78 (Nuclear intron maturase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT4 PE=3 SV=2)

HSP 1 Score: 875.5 bits (2261), Expect = 4.4e-253
Identity = 447/743 (60.16%), Postives = 554/743 (74.56%), Query Frame = 0

Query: 56  LAINLASLVEESL--VVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACP 115
           LA  LASLVEES   V D  +P++RMEL+RSLE+++KKRVK Q +NGKF DL+ KVIA P
Sbjct: 56  LAGELASLVEESSSHVDDDSKPRSRMELKRSLELRLKKRVKEQCINGKFSDLLKKVIARP 115

Query: 116 TTLQNAYDCIRLNSNVDIASNDHLMSFDSMAEELRNGSFDVNVNTFSI--SSSRKEVLIL 175
            TL++AYDCIRLNSNV I   +  ++FDS+AEEL +G FDV  NTFSI      KEVL+L
Sbjct: 116 ETLRDAYDCIRLNSNVSITERNGSVAFDSIAEELSSGVFDVASNTFSIVARDKTKEVLVL 175

Query: 176 PKLKLKVLQEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLS 235
           P + LKV+QEAIRIVLE VF PHFSKISH CRSGRG ++ALKYI   I   DW FTL L+
Sbjct: 176 PSVALKVVQEAIRIVLEVVFSPHFSKISHSCRSGRGRASALKYINNNISRSDWCFTLSLN 235

Query: 236 KKMDELVMAKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPIL 295
           KK+D  V   +++VME+K+ED +L  ++RS+F+A  LNLEFGGFPKGHGLPQEGVLS +L
Sbjct: 236 KKLDVSVFENLLSVMEEKVEDSSLSILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVL 295

Query: 296 MNIYLNLFDQEFFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNI 355
           MNIYL+ FD EF+R+SM++EA+        D+  SKLRSWFRR       +   E+   +
Sbjct: 296 MNIYLDRFDHEFYRISMRHEALGLDSKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVAL 355

Query: 356 RVYCCRYMDEIFLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLG 415
           RVYCCR+MDEI+ +VSG K V    RSE + F+  SLHLD+  + +   C  T G+R LG
Sbjct: 356 RVYCCRFMDEIYFSVSGPKKVASDIRSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLG 415

Query: 416 CLVRRSMKESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKH 475
            LVR++++ESP VKAVHKLKEKV LFALQK+EAW   TV +GKKWL HGLKKVKESEIK 
Sbjct: 416 TLVRKNVRESPTVKAVHKLKEKVRLFALQKEEAWTLGTVRIGKKWLGHGLKKVKESEIKG 475

Query: 476 LAKNSPSLNQISSFRKAGMETDHWYKVLLKIWMQDI-NAKAAESEETILSNHVVQPSLPL 535
           LA ++ +L+QIS  RKAGMETDHWYK+LL+IWM+D+    A  SEE +LS HVV+P++P 
Sbjct: 476 LADSNSTLSQISCHRKAGMETDHWYKILLRIWMEDVLRTSADRSEEFVLSKHVVEPTVPQ 535

Query: 536 ELRDSFHEFQRCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKRLFRYRLI 595
           ELRD+F++FQ     YVSSETA+  ALLP      +  F  +++AP ++I +RL+RY LI
Sbjct: 536 ELRDAFYKFQNAAAAYVSSETANLEALLPCPQSHDRPVFFGDVVAPTNAIGRRLYRYGLI 595

Query: 596 TNKGYPCPSPFLILQDNNQIIDWFSGVSRRWLQWYSICSNFSEL-ILICDQVRKSCIRTL 655
           T KGY   +  LIL D  QIIDW+SG+ RRW+ WY  CSNF E+  LI +Q+R SCIRTL
Sbjct: 596 TAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSNFDEIKALIDNQIRMSCIRTL 655

Query: 656 AAKHRIHESEIEKKFDSELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGISYSGLCLL 715
           AAK+RIHE+EIEK+ D ELS I S+ ++EQE +    D+   D DE L YG+S SGLCLL
Sbjct: 656 AAKYRIHENEIEKRLDLELSTIPSAEDIEQEIQHEKLDSPAFDRDEHLTYGLSNSGLCLL 715

Query: 716 SLARMVSQSRPCNCFVIGCLVPAPSVYTLHVMERQKFPGWNTGFSSSIHPSLNRRRFGLC 775
           SLAR+VS+SRPCNCFVIGC + AP+VYTLH MERQKFPGW TGFS  I  SLN RR GLC
Sbjct: 716 SLARLVSESRPCNCFVIGCSMAAPAVYTLHAMERQKFPGWKTGFSVCIPSSLNGRRIGLC 775

Query: 776 KQHLKDLYLGHISLQSIDFGAWK 793
           KQHLKDLY+G ISLQ++DFGAW+
Sbjct: 776 KQHLKDLYIGQISLQAVDFGAWR 798

BLAST of Lag0032616 vs. ExPASy Swiss-Prot
Match: Q9LZA5 (Nuclear intron maturase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT3 PE=3 SV=2)

HSP 1 Score: 223.8 bits (569), Expect = 6.9e-57
Identity = 203/770 (26.36%), Postives = 336/770 (43.64%), Query Frame = 0

Query: 67  SLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNAYDCIRL- 126
           SL ++  +  T   ++  LE  V K    QY +GKF  L+   ++ P  L  A   + L 
Sbjct: 31  SLFLNSDQTITEPLVKSELEALVLK----QYSHGKFYSLVKNAVSLPCVLLAACQNLSLS 90

Query: 127 -NSNVDIASN-DHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVLQEAI 186
            NS+ D+A       S + M  E+R G FD+        SS    L+LP LKLKVL EAI
Sbjct: 91  ANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISSS---LVLPNLKLKVLIEAI 150

Query: 187 RIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVMAKII 246
           R+VLE V+   F+  S+G R G G  TA++Y++  ++NP WWF +  +++M E     I+
Sbjct: 151 RMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNVDIL 210

Query: 247 T-VMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLFDQE 306
              + +KI D  L  +I+ +F+ G L +E GG   G G PQE  L  IL+N+Y +  D+E
Sbjct: 211 CGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGLDKE 270

Query: 307 FFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYMDEI 366
              L +K +  N     G + S             GN    P      + +Y  RY+DEI
Sbjct: 271 IQDLRLKMKVKNPRVGTGDEES------------TGNVFFKP------VNIYAVRYLDEI 330

Query: 367 FLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCL-------VR 426
            +  SGSK +T+  +  I++ + + L L ++     +  A +  + FLG         V 
Sbjct: 331 LVITSGSKMLTMDLKKRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVL 390

Query: 427 RSMKESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKN 486
           R  K   AV+A+ K + + ++  L+ + A       LG K   H LKK+K+S        
Sbjct: 391 RPPKSEKAVRAMKKYQRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQS-------- 450

Query: 487 SPSLNQISSFRKAGMETDHWYKVLLKIW----MQDINAKAAE--------SEETILSNHV 546
                  + F+  G E ++  + + + W    MQD      E        +    LS   
Sbjct: 451 -------NGFKFEG-EIENEVRDIFQSWGEEVMQDFMGSLEERWKWHWLLTRGDFLSLRH 510

Query: 547 VQPSLPLELRDSFHEFQRCVEQYVSSETASTIALLPNYDPSVK---------------ST 606
           ++  LP +L D++ EFQ  V+++++   A  +  L + +  V+               + 
Sbjct: 511 IREKLPQDLIDAYDEFQEQVDKHLAPTQAKKV--LEDEERRVEEEEEQRYAERTVEDLTK 570

Query: 607 FITEIIAPVDSIRKRLFRYRLITNKGYPCPSPFLILQDNNQIIDWFS-----GVSRRWLQ 666
              ++ AP + +RK +       + G P P   L+  +++ II W++     G +++ ++
Sbjct: 571 LCMKVSAPEELVRKAIKLVGFTNSMGRPRPIIHLVTLEDSDIIKWYARHEKHGSTKKLIR 630

Query: 667 WYSICSNFSELILICDQVRKSCIRTLAAKHRIHESEIEKKFDSELSRIYSSPEVEQEEEK 726
            Y+     S+L                      +   E  F SE        EV+   +K
Sbjct: 631 HYTKDLRVSDL----------------------DGREEAHFPSE-------REVKMMGDK 690

Query: 727 RTSDTHVLDHDEALMYGISYSGLCLLSLARMVSQSRPCNCFVIGCLVPAPSVYTLHVMER 786
             SD   +D            G   L L R+ S     +C    C      ++ +H+++ 
Sbjct: 691 NLSDPKPVD------------GTLSLLLIRLASDEPLHHCAASFCERSDTIMHRVHLLQN 715

Query: 787 QKF------PGWNTGFSSSIHPSLNRRRFGLCKQHLKDLYLGHISLQSID 788
           +          W  G   +IH +LNR+   LC  H+ D+YLG I+LQ +D
Sbjct: 751 RLHINPLDEEKWVPGM-GTIHSALNRKCLPLCSTHISDVYLGKITLQDVD 715

BLAST of Lag0032616 vs. ExPASy Swiss-Prot
Match: P0A3U0 (Group II intron-encoded protein LtrA OS=Lactococcus lactis subsp. cremoris OX=1359 GN=ltrA PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 4.3e-22
Identity = 84/291 (28.87%), Postives = 146/291 (50.17%), Query Frame = 0

Query: 162 SSSRKEVLILPKLKLKVLQEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDN 221
           +S +   L +P    K++QEA+RI+LE ++ P F  +SHG R  R   TALK I++E   
Sbjct: 93  NSKKMRPLGIPTFTDKLIQEAVRIILESIYEPVFEDVSHGFRPQRSCHTALKTIKREFGG 152

Query: 222 PDWWFTLDLSKKMDELVMAKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGH-G 281
             W+   D+    D +    +I ++  KI+D  +  +I     AG   LE   + K + G
Sbjct: 153 ARWFVEGDIKGCFDNIDHVTLIGLINLKIKDMKMSQLIYKFLKAG--YLENWQYHKTYSG 212

Query: 282 LPQEGVLSPILMNIYLNLFDQEFFRLSMKY--EAINKYGNAGRDA-SQSKLRSWFRRHLK 341
            PQ G+LSP+L NIYL+  D+   +L MK+  E+  +     R+  ++ K  S   + L+
Sbjct: 213 TPQGGILSPLLANIYLHELDKFVLQLKMKFDRESPERITPEYRELHNEIKRISHRLKKLE 272

Query: 342 GNDS-----EYPGEEKDNIRVYC----------CRYMDEIFLAVSGSKDVTISFRSEILE 401
           G +      EY  + K    + C           RY D+  ++V GSK+     + ++  
Sbjct: 273 GEEKAKVLLEYQEKRKRLPTLPCTSQTNKVLKYVRYADDFIISVKGSKEDCQWIKEQLKL 332

Query: 402 FIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMKESPAVKAVHKLKEK 434
           FIH  L ++L+ +  +++   ++  RFLG  +R  ++ S  +K   K+K++
Sbjct: 333 FIHNKLKMELSEEKTLIT-HSSQPARFLGYDIR--VRRSGTIKRSGKVKKR 378

BLAST of Lag0032616 vs. ExPASy Swiss-Prot
Match: P0A3U1 (Group II intron-encoded protein LtrA OS=Lactococcus lactis subsp. cremoris (strain MG1363) OX=416870 GN=ltrA PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 4.3e-22
Identity = 84/291 (28.87%), Postives = 146/291 (50.17%), Query Frame = 0

Query: 162 SSSRKEVLILPKLKLKVLQEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDN 221
           +S +   L +P    K++QEA+RI+LE ++ P F  +SHG R  R   TALK I++E   
Sbjct: 93  NSKKMRPLGIPTFTDKLIQEAVRIILESIYEPVFEDVSHGFRPQRSCHTALKTIKREFGG 152

Query: 222 PDWWFTLDLSKKMDELVMAKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGH-G 281
             W+   D+    D +    +I ++  KI+D  +  +I     AG   LE   + K + G
Sbjct: 153 ARWFVEGDIKGCFDNIDHVTLIGLINLKIKDMKMSQLIYKFLKAG--YLENWQYHKTYSG 212

Query: 282 LPQEGVLSPILMNIYLNLFDQEFFRLSMKY--EAINKYGNAGRDA-SQSKLRSWFRRHLK 341
            PQ G+LSP+L NIYL+  D+   +L MK+  E+  +     R+  ++ K  S   + L+
Sbjct: 213 TPQGGILSPLLANIYLHELDKFVLQLKMKFDRESPERITPEYRELHNEIKRISHRLKKLE 272

Query: 342 GNDS-----EYPGEEKDNIRVYC----------CRYMDEIFLAVSGSKDVTISFRSEILE 401
           G +      EY  + K    + C           RY D+  ++V GSK+     + ++  
Sbjct: 273 GEEKAKVLLEYQEKRKRLPTLPCTSQTNKVLKYVRYADDFIISVKGSKEDCQWIKEQLKL 332

Query: 402 FIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMKESPAVKAVHKLKEK 434
           FIH  L ++L+ +  +++   ++  RFLG  +R  ++ S  +K   K+K++
Sbjct: 333 FIHNKLKMELSEEKTLIT-HSSQPARFLGYDIR--VRRSGTIKRSGKVKKR 378

BLAST of Lag0032616 vs. ExPASy Swiss-Prot
Match: B1N1A3 (Putative nicotine oxidoreductase OS=Pseudomonas putida OX=303 GN=nicA PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.2e-19
Identity = 82/283 (28.98%), Postives = 133/283 (47.00%), Query Frame = 0

Query: 177 KVLQEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDE 236
           KV+QE IR +LE ++ P FSK SHG R+G+   TALK +R+      W    D+    D 
Sbjct: 109 KVVQEVIRSILEAIYEPTFSKNSHGFRAGKSCHTALKQVRESWSGVTWVIEGDIKGCFDN 168

Query: 237 LVMAKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGH-GLPQEGVLSPILMNIY 296
           +  +K+I  +  +I+D     +IR   +AG    E G F     G PQ  ++SPIL N++
Sbjct: 169 ISHSKLIDQLRLRIKDERFINLIRKALNAG--YFENGAFFSATLGTPQGSIISPILANVF 228

Query: 297 LNLFDQEFFRL--------------------------SMKYEAINKYGNAGRDASQSKLR 356
           L+  D++  +L                          S++ +A  K   A RDA+ S  R
Sbjct: 229 LDQLDRKVEQLIKDHHQGEEGDKITDPAYRKLQRQKTSLRKKA-EKQEGAERDATLSLAR 288

Query: 357 SWFRRHLKGNDSEYPGEEKDNIRVYCCRYMDEIFLAVSGSKDVTISFRSEILEFIHKSLH 416
               + L  + S Y       IRV   RY D+  + V+G K +    RS + EF+ ++  
Sbjct: 289 EANSKLL--SMSPYLTRNNGFIRVKYVRYADDWIIGVNGPKLLAEELRSVVGEFL-ENAG 348

Query: 417 LDLNHQGEMVSCAETRGVRFLGCLVRRSMKESPAVKAVHKLKE 433
           L+L+ +   +  A++   +FLG  +R   + S  +K +   K+
Sbjct: 349 LELSIEKTHIRHAKSETAKFLGTNLRIGSENSKIMKVLRNGKK 385

HSP 2 Score: 44.7 bits (104), Expect = 5.8e-03
Identity = 34/124 (27.42%), Postives = 56/124 (45.16%), Query Frame = 0

Query: 547 VSSETASTIALLPNYD--PSVKSTFITEIIAPVDSIRKRLFRYRLITNKGYPCPSPFLIL 606
           + SE +  + +L N    P   + +   + AP++ +  +L        KG P      I 
Sbjct: 369 IGSENSKIMKVLRNGKKFPKRVAGWTPVMYAPMNELIAKLHSRGFCDPKGNPTTVNKWIF 428

Query: 607 QDNNQIIDWFSGVSRRWLQWYSICSNFSELILICDQVRKSCIRTLAAKHRIHESEIEKKF 666
            D+NQI++    V R  + +YS    FS L  I   ++ +  +TLA KHR   S+I  K 
Sbjct: 429 LDDNQIVEQIGSVWRGIMNYYSFIDQFSMLSRIQFILQHAAAKTLATKHRSSRSKIFSKH 488

Query: 667 DSEL 669
            + L
Sbjct: 489 GANL 492

BLAST of Lag0032616 vs. ExPASy TrEMBL
Match: A0A6J1CXL0 (nuclear intron maturase 4, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015360 PE=4 SV=1)

HSP 1 Score: 1423.3 bits (3683), Expect = 0.0e+00
Identity = 702/792 (88.64%), Postives = 744/792 (93.94%), Query Frame = 0

Query: 1   MNMQNFAVLRSVHCKVNSSFVSDIGKCVQRVQSSENYSALACVDDEIDKGMEKKKLAINL 60
           MNM+NFAVLR   CKVNSSFVSDIGKCVQRVQ+SENYSALAC DD+  KGMEKKKLA NL
Sbjct: 20  MNMRNFAVLRI--CKVNSSFVSDIGKCVQRVQTSENYSALACADDDFCKGMEKKKLAENL 79

Query: 61  ASLVEESLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNAY 120
           ASLVEESL VD +RPK+RMEL+RSLEIQ+KKRVKAQY+NGKFMDLMGKVIACP TLQNAY
Sbjct: 80  ASLVEESLDVDSRRPKSRMELKRSLEIQIKKRVKAQYVNGKFMDLMGKVIACPPTLQNAY 139

Query: 121 DCIRLNSNVDIASNDHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVLQ 180
           DC+R+NSNVDIASNDHL+SF+SMAEEL NGSFDVN NTFSISSS+KEVLILPKLKLKVLQ
Sbjct: 140 DCVRINSNVDIASNDHLISFESMAEELHNGSFDVNANTFSISSSKKEVLILPKLKLKVLQ 199

Query: 181 EAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVMA 240
           EAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEI+NPDWWFT+D+SKKMDEL MA
Sbjct: 200 EAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEINNPDWWFTVDISKKMDELEMA 259

Query: 241 KIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLFD 300
           K+I+VMEDKIEDP  FA+IRSIF+AGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLFD
Sbjct: 260 KLISVMEDKIEDPEFFAIIRSIFEAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLFD 319

Query: 301 QEFFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYMD 360
           QEFFRLSMKYEAINKYGNA +D SQSKLRSWFRR LKGNDSEYP +EKDNIRVYCCRYMD
Sbjct: 320 QEFFRLSMKYEAINKYGNAVQDGSQSKLRSWFRRKLKGNDSEYPAQEKDNIRVYCCRYMD 379

Query: 361 EIFLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMKE 420
           EIF+AVSGSKDV +SFRSEI +FI KSLHLD+NHQ EMVSC ETRG+RFLGCLVRRS KE
Sbjct: 380 EIFMAVSGSKDVALSFRSEIQDFIQKSLHLDVNHQEEMVSCRETRGIRFLGCLVRRSEKE 439

Query: 421 SPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSLN 480
           SPAVKAVHKLKEKVELFALQKQEAWN WTVWLGKKWLAHGLKKVKESEIKHLAKNSPSLN
Sbjct: 440 SPAVKAVHKLKEKVELFALQKQEAWNDWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSLN 499

Query: 481 QISSFRKAGMETDHWYKVLLKIWMQDINAKAAESEETILSNHVVQPSLPLELRDSFHEFQ 540
           QISSFRK GMETDHWYKVLLKIWMQDINAKAAE+EETILSN+VV+PSLPLELRDSF+EFQ
Sbjct: 500 QISSFRKVGMETDHWYKVLLKIWMQDINAKAAETEETILSNYVVEPSLPLELRDSFYEFQ 559

Query: 541 RCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKRLFRYRLITNKGYPCPSP 600
           R VE+YVSSETAST+ALLPNYDPSVKSTFITEIIAPV+SIRKRL RYRLITNKGYPC SP
Sbjct: 560 RRVEEYVSSETASTVALLPNYDPSVKSTFITEIIAPVNSIRKRLLRYRLITNKGYPCASP 619

Query: 601 FLILQDNNQIIDWFSGVSRRWLQWYSICSNFSELILICDQVRKSCIRTLAAKHRIHESEI 660
           FLIL DN QIIDWF GV RRWL+WYS CSNFSE+ILICDQVRKSCIRTLAAKHR HESEI
Sbjct: 620 FLILHDNTQIIDWFLGVYRRWLKWYSNCSNFSEVILICDQVRKSCIRTLAAKHRTHESEI 679

Query: 661 EKKFDSELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGISYSGLCLLSLARMVSQSRP 720
           EKKFD ELSRI S+PE+EQEEE+  SDTH L HDEA  YGISYSGLCLLSLARMVSQSRP
Sbjct: 680 EKKFDLELSRICSTPEIEQEEEEEASDTHGLGHDEASTYGISYSGLCLLSLARMVSQSRP 739

Query: 721 CNCFVIGCLVPAPSVYTLHVMERQKFPGWNTGFSSSIHPSLNRRRFGLCKQHLKDLYLGH 780
           CNCFV+GCL  APSVYTLHVMERQKFPGW TGFSSSIHPSLNRRR GLCKQHLKDLYLGH
Sbjct: 740 CNCFVMGCLASAPSVYTLHVMERQKFPGWKTGFSSSIHPSLNRRRVGLCKQHLKDLYLGH 799

Query: 781 ISLQSIDFGAWK 793
           ISLQS++FGAWK
Sbjct: 800 ISLQSVNFGAWK 809

BLAST of Lag0032616 vs. ExPASy TrEMBL
Match: A0A6J1CYJ7 (nuclear intron maturase 4, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015360 PE=4 SV=1)

HSP 1 Score: 1418.7 bits (3671), Expect = 0.0e+00
Identity = 702/793 (88.52%), Postives = 744/793 (93.82%), Query Frame = 0

Query: 1   MNMQNFAVLRSVHCKVNSSFVSDI-GKCVQRVQSSENYSALACVDDEIDKGMEKKKLAIN 60
           MNM+NFAVLR   CKVNSSFVSDI GKCVQRVQ+SENYSALAC DD+  KGMEKKKLA N
Sbjct: 20  MNMRNFAVLRI--CKVNSSFVSDIAGKCVQRVQTSENYSALACADDDFCKGMEKKKLAEN 79

Query: 61  LASLVEESLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNA 120
           LASLVEESL VD +RPK+RMEL+RSLEIQ+KKRVKAQY+NGKFMDLMGKVIACP TLQNA
Sbjct: 80  LASLVEESLDVDSRRPKSRMELKRSLEIQIKKRVKAQYVNGKFMDLMGKVIACPPTLQNA 139

Query: 121 YDCIRLNSNVDIASNDHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVL 180
           YDC+R+NSNVDIASNDHL+SF+SMAEEL NGSFDVN NTFSISSS+KEVLILPKLKLKVL
Sbjct: 140 YDCVRINSNVDIASNDHLISFESMAEELHNGSFDVNANTFSISSSKKEVLILPKLKLKVL 199

Query: 181 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVM 240
           QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEI+NPDWWFT+D+SKKMDEL M
Sbjct: 200 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEINNPDWWFTVDISKKMDELEM 259

Query: 241 AKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF 300
           AK+I+VMEDKIEDP  FA+IRSIF+AGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF
Sbjct: 260 AKLISVMEDKIEDPEFFAIIRSIFEAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF 319

Query: 301 DQEFFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYM 360
           DQEFFRLSMKYEAINKYGNA +D SQSKLRSWFRR LKGNDSEYP +EKDNIRVYCCRYM
Sbjct: 320 DQEFFRLSMKYEAINKYGNAVQDGSQSKLRSWFRRKLKGNDSEYPAQEKDNIRVYCCRYM 379

Query: 361 DEIFLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMK 420
           DEIF+AVSGSKDV +SFRSEI +FI KSLHLD+NHQ EMVSC ETRG+RFLGCLVRRS K
Sbjct: 380 DEIFMAVSGSKDVALSFRSEIQDFIQKSLHLDVNHQEEMVSCRETRGIRFLGCLVRRSEK 439

Query: 421 ESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL 480
           ESPAVKAVHKLKEKVELFALQKQEAWN WTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL
Sbjct: 440 ESPAVKAVHKLKEKVELFALQKQEAWNDWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL 499

Query: 481 NQISSFRKAGMETDHWYKVLLKIWMQDINAKAAESEETILSNHVVQPSLPLELRDSFHEF 540
           NQISSFRK GMETDHWYKVLLKIWMQDINAKAAE+EETILSN+VV+PSLPLELRDSF+EF
Sbjct: 500 NQISSFRKVGMETDHWYKVLLKIWMQDINAKAAETEETILSNYVVEPSLPLELRDSFYEF 559

Query: 541 QRCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKRLFRYRLITNKGYPCPS 600
           QR VE+YVSSETAST+ALLPNYDPSVKSTFITEIIAPV+SIRKRL RYRLITNKGYPC S
Sbjct: 560 QRRVEEYVSSETASTVALLPNYDPSVKSTFITEIIAPVNSIRKRLLRYRLITNKGYPCAS 619

Query: 601 PFLILQDNNQIIDWFSGVSRRWLQWYSICSNFSELILICDQVRKSCIRTLAAKHRIHESE 660
           PFLIL DN QIIDWF GV RRWL+WYS CSNFSE+ILICDQVRKSCIRTLAAKHR HESE
Sbjct: 620 PFLILHDNTQIIDWFLGVYRRWLKWYSNCSNFSEVILICDQVRKSCIRTLAAKHRTHESE 679

Query: 661 IEKKFDSELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGISYSGLCLLSLARMVSQSR 720
           IEKKFD ELSRI S+PE+EQEEE+  SDTH L HDEA  YGISYSGLCLLSLARMVSQSR
Sbjct: 680 IEKKFDLELSRICSTPEIEQEEEEEASDTHGLGHDEASTYGISYSGLCLLSLARMVSQSR 739

Query: 721 PCNCFVIGCLVPAPSVYTLHVMERQKFPGWNTGFSSSIHPSLNRRRFGLCKQHLKDLYLG 780
           PCNCFV+GCL  APSVYTLHVMERQKFPGW TGFSSSIHPSLNRRR GLCKQHLKDLYLG
Sbjct: 740 PCNCFVMGCLASAPSVYTLHVMERQKFPGWKTGFSSSIHPSLNRRRVGLCKQHLKDLYLG 799

Query: 781 HISLQSIDFGAWK 793
           HISLQS++FGAWK
Sbjct: 800 HISLQSVNFGAWK 810

BLAST of Lag0032616 vs. ExPASy TrEMBL
Match: A0A6J1CX32 (nuclear intron maturase 4, mitochondrial isoform X3 OS=Momordica charantia OX=3673 GN=LOC111015360 PE=4 SV=1)

HSP 1 Score: 1418.7 bits (3671), Expect = 0.0e+00
Identity = 702/793 (88.52%), Postives = 744/793 (93.82%), Query Frame = 0

Query: 1   MNMQNFAVLRSVHCKVNSSFVSDI-GKCVQRVQSSENYSALACVDDEIDKGMEKKKLAIN 60
           MNM+NFAVLR   CKVNSSFVSDI GKCVQRVQ+SENYSALAC DD+  KGMEKKKLA N
Sbjct: 12  MNMRNFAVLRI--CKVNSSFVSDIAGKCVQRVQTSENYSALACADDDFCKGMEKKKLAEN 71

Query: 61  LASLVEESLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNA 120
           LASLVEESL VD +RPK+RMEL+RSLEIQ+KKRVKAQY+NGKFMDLMGKVIACP TLQNA
Sbjct: 72  LASLVEESLDVDSRRPKSRMELKRSLEIQIKKRVKAQYVNGKFMDLMGKVIACPPTLQNA 131

Query: 121 YDCIRLNSNVDIASNDHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVL 180
           YDC+R+NSNVDIASNDHL+SF+SMAEEL NGSFDVN NTFSISSS+KEVLILPKLKLKVL
Sbjct: 132 YDCVRINSNVDIASNDHLISFESMAEELHNGSFDVNANTFSISSSKKEVLILPKLKLKVL 191

Query: 181 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVM 240
           QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEI+NPDWWFT+D+SKKMDEL M
Sbjct: 192 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEINNPDWWFTVDISKKMDELEM 251

Query: 241 AKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF 300
           AK+I+VMEDKIEDP  FA+IRSIF+AGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF
Sbjct: 252 AKLISVMEDKIEDPEFFAIIRSIFEAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF 311

Query: 301 DQEFFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYM 360
           DQEFFRLSMKYEAINKYGNA +D SQSKLRSWFRR LKGNDSEYP +EKDNIRVYCCRYM
Sbjct: 312 DQEFFRLSMKYEAINKYGNAVQDGSQSKLRSWFRRKLKGNDSEYPAQEKDNIRVYCCRYM 371

Query: 361 DEIFLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMK 420
           DEIF+AVSGSKDV +SFRSEI +FI KSLHLD+NHQ EMVSC ETRG+RFLGCLVRRS K
Sbjct: 372 DEIFMAVSGSKDVALSFRSEIQDFIQKSLHLDVNHQEEMVSCRETRGIRFLGCLVRRSEK 431

Query: 421 ESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL 480
           ESPAVKAVHKLKEKVELFALQKQEAWN WTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL
Sbjct: 432 ESPAVKAVHKLKEKVELFALQKQEAWNDWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL 491

Query: 481 NQISSFRKAGMETDHWYKVLLKIWMQDINAKAAESEETILSNHVVQPSLPLELRDSFHEF 540
           NQISSFRK GMETDHWYKVLLKIWMQDINAKAAE+EETILSN+VV+PSLPLELRDSF+EF
Sbjct: 492 NQISSFRKVGMETDHWYKVLLKIWMQDINAKAAETEETILSNYVVEPSLPLELRDSFYEF 551

Query: 541 QRCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKRLFRYRLITNKGYPCPS 600
           QR VE+YVSSETAST+ALLPNYDPSVKSTFITEIIAPV+SIRKRL RYRLITNKGYPC S
Sbjct: 552 QRRVEEYVSSETASTVALLPNYDPSVKSTFITEIIAPVNSIRKRLLRYRLITNKGYPCAS 611

Query: 601 PFLILQDNNQIIDWFSGVSRRWLQWYSICSNFSELILICDQVRKSCIRTLAAKHRIHESE 660
           PFLIL DN QIIDWF GV RRWL+WYS CSNFSE+ILICDQVRKSCIRTLAAKHR HESE
Sbjct: 612 PFLILHDNTQIIDWFLGVYRRWLKWYSNCSNFSEVILICDQVRKSCIRTLAAKHRTHESE 671

Query: 661 IEKKFDSELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGISYSGLCLLSLARMVSQSR 720
           IEKKFD ELSRI S+PE+EQEEE+  SDTH L HDEA  YGISYSGLCLLSLARMVSQSR
Sbjct: 672 IEKKFDLELSRICSTPEIEQEEEEEASDTHGLGHDEASTYGISYSGLCLLSLARMVSQSR 731

Query: 721 PCNCFVIGCLVPAPSVYTLHVMERQKFPGWNTGFSSSIHPSLNRRRFGLCKQHLKDLYLG 780
           PCNCFV+GCL  APSVYTLHVMERQKFPGW TGFSSSIHPSLNRRR GLCKQHLKDLYLG
Sbjct: 732 PCNCFVMGCLASAPSVYTLHVMERQKFPGWKTGFSSSIHPSLNRRRVGLCKQHLKDLYLG 791

Query: 781 HISLQSIDFGAWK 793
           HISLQS++FGAWK
Sbjct: 792 HISLQSVNFGAWK 802

BLAST of Lag0032616 vs. ExPASy TrEMBL
Match: A0A1S3B491 (uncharacterized protein LOC103486008 OS=Cucumis melo OX=3656 GN=LOC103486008 PE=4 SV=1)

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 671/793 (84.62%), Postives = 729/793 (91.93%), Query Frame = 0

Query: 1   MNMQNFAVLRSVH-CKVNSSFVSDIGKCVQRVQSSENYSALACVDDEIDKGMEKKKLAIN 60
           +N +NF+  +SV+ C VNSSFVSDIGKC Q VQSSENYS LA  DDEIDKGMEK KLA+N
Sbjct: 12  INKRNFSNSQSVNVCIVNSSFVSDIGKCFQIVQSSENYSTLARADDEIDKGMEKMKLAMN 71

Query: 61  LASLVEESLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNA 120
           LASLVEESL VDL+R KTRMEL+RSLEIQ+K+RVKAQY+NGKF+DLMG VIACP TLQNA
Sbjct: 72  LASLVEESLDVDLRRSKTRMELKRSLEIQIKERVKAQYLNGKFLDLMGNVIACPNTLQNA 131

Query: 121 YDCIRLNSNVDIASNDHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVL 180
           YDCIR+NSNVDI SND L+SF+SMA+EL +G+FDVN NTFSI SSRKEVLILPK+KLKVL
Sbjct: 132 YDCIRINSNVDIKSNDCLISFESMAKELSHGNFDVNTNTFSILSSRKEVLILPKIKLKVL 191

Query: 181 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVM 240
           QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYI+KEI +PDWWFT+DLSKKMDELVM
Sbjct: 192 QEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIKKEIKDPDWWFTVDLSKKMDELVM 251

Query: 241 AKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLF 300
           AK+ITVMEDKIEDP LFAVIRSI  AGALNLEFG FPKGHGLPQEGVLSPIL NIYLNLF
Sbjct: 252 AKLITVMEDKIEDPKLFAVIRSIHLAGALNLEFGSFPKGHGLPQEGVLSPILTNIYLNLF 311

Query: 301 DQEFFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYM 360
           DQEFFRLSMKYEAIN+YGN G+D SQSKLRSWFRR LK N S+YPGEEKD IRVYCCRYM
Sbjct: 312 DQEFFRLSMKYEAINEYGNTGQDGSQSKLRSWFRRQLKENSSDYPGEEKDKIRVYCCRYM 371

Query: 361 DEIFLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMK 420
           DEIFLAVSGSKDV +SFRSEI +F+ K+LHLD+NH+ EMVSC ET G+RFLGCLVRRS++
Sbjct: 372 DEIFLAVSGSKDVALSFRSEIFDFMQKTLHLDVNHEEEMVSC-ETHGIRFLGCLVRRSVQ 431

Query: 421 ESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSL 480
           ESPAVK++HKLKEKVELF LQKQE W +WTVWLGKKWLAHGLKKVKESEIKHLAKNS SL
Sbjct: 432 ESPAVKSIHKLKEKVELFGLQKQETWKSWTVWLGKKWLAHGLKKVKESEIKHLAKNS-SL 491

Query: 481 NQISSFRKAGMETDHWYKVLLKIWMQDINAKAAESEETILSNHVVQPSLPLELRDSFHEF 540
           NQISSFRK GMETDHWYKVLLKIWMQD+NA+AAESEE ILS H V+PSLP ELRDSF+EF
Sbjct: 492 NQISSFRKPGMETDHWYKVLLKIWMQDLNARAAESEEKILSKHAVEPSLPFELRDSFYEF 551

Query: 541 QRCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKRLFRYRLITNKGYPCPS 600
           QR VE+Y+SSETAST+ALLPNYDPSVK TFITEIIAPV+SIRKRLFRYRL+TNKG+PC S
Sbjct: 552 QRRVEEYISSETASTLALLPNYDPSVKPTFITEIIAPVNSIRKRLFRYRLVTNKGHPCSS 611

Query: 601 PFLILQDNNQIIDWFSGVSRRWLQWYSICSNFSELILICDQVRKSCIRTLAAKHRIHESE 660
           PFLILQDN QIIDWF GVSRRW +WY+  SNFSEL LI DQVRKSCIRTLAAKH+IHESE
Sbjct: 612 PFLILQDNTQIIDWFLGVSRRWFRWYNKSSNFSELFLIFDQVRKSCIRTLAAKHQIHESE 671

Query: 661 IEKKFDSELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGISYSGLCLLSLARMVSQSR 720
           IEKKFDSELS+IYSSPE+EQE+EK T DTHVLDHDEAL YGISYSGLCLLSLARMVS+SR
Sbjct: 672 IEKKFDSELSKIYSSPEIEQEKEKST-DTHVLDHDEALNYGISYSGLCLLSLARMVSRSR 731

Query: 721 PCNCFVIGCLVPAPSVYTLHVMERQKFPGWNTGFSSSIHPSLNRRRFGLCKQHLKDLYLG 780
           PCNCFV+GCL PAPSVYTLHVMERQKFPGW TGFSSSIHPSLN+RRFGLCKQHL DLYLG
Sbjct: 732 PCNCFVVGCLAPAPSVYTLHVMERQKFPGWKTGFSSSIHPSLNKRRFGLCKQHLADLYLG 791

Query: 781 HISLQSIDFGAWK 793
            ISLQS+DFGAWK
Sbjct: 792 RISLQSVDFGAWK 801

BLAST of Lag0032616 vs. ExPASy TrEMBL
Match: A0A6J1EMZ4 (nuclear intron maturase 4, mitochondrial isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435824 PE=4 SV=1)

HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 669/830 (80.60%), Postives = 740/830 (89.16%), Query Frame = 0

Query: 1   MNMQNFAVLRSVH-CKVNSSFVSDIGKCVQRVQSSENYSALACVDDEI------------ 60
           +NM++F+V RSV+ C+V++SFVS+IG+C   VQSS+NYSALA  DDEI            
Sbjct: 12  INMRSFSVSRSVNACRVDTSFVSEIGEC---VQSSKNYSALAFTDDEIGKGTEKRKLAMN 71

Query: 61  --------------------------DKGMEKKKLAINLASLVEESLVVDLKRPKTRMEL 120
                                      KGMEK KLA+NLASLVEESL VD K  KTRMEL
Sbjct: 72  LASLVEESIDVDMKTSKTRMELERSLGKGMEKSKLAMNLASLVEESLDVDTKTSKTRMEL 131

Query: 121 RRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNAYDCIRLNSNVDIASNDHLMSFD 180
           +RSLEIQ+KKRVKAQY+NGKF+DLM KV+ACP TLQNAY+C+R+NSNVD+ SNDHL+SF+
Sbjct: 132 KRSLEIQIKKRVKAQYVNGKFLDLMEKVVACPKTLQNAYNCVRINSNVDVTSNDHLISFE 191

Query: 181 SMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVLQEAIRIVLECVFRPHFSKISHG 240
            MAEELRNG+FD+N NTFSISSSRKEVLILPKLKLKVLQEAIRIVLECVFRPHFSKISHG
Sbjct: 192 PMAEELRNGNFDINANTFSISSSRKEVLILPKLKLKVLQEAIRIVLECVFRPHFSKISHG 251

Query: 241 CRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVMAKIITVMEDKIEDPNLFAVIRS 300
           CRSGRGHSTALKYI+KEI NPDWWFT++LSK MDEL+MAK+ITVM+DKIEDPNLFAVIR+
Sbjct: 252 CRSGRGHSTALKYIKKEIKNPDWWFTVNLSKMMDELMMAKLITVMKDKIEDPNLFAVIRT 311

Query: 301 IFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLFDQEFFRLSMKYEAINKYGNAGR 360
           IFDAGALNLEFG FPKGHGLPQEGVLSPILMNIYLNLFDQEFFRLSMKYEAIN+Y NAG+
Sbjct: 312 IFDAGALNLEFGDFPKGHGLPQEGVLSPILMNIYLNLFDQEFFRLSMKYEAINEYSNAGQ 371

Query: 361 DASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYMDEIFLAVSGSKDVTISFRSEIL 420
             SQSKLRSWFRR LKGNDSEYPG+EK NIRVYCCR MDEIFLA+SGSKDV + FRSEIL
Sbjct: 372 GGSQSKLRSWFRRQLKGNDSEYPGDEKGNIRVYCCRCMDEIFLAISGSKDVALRFRSEIL 431

Query: 421 EFIHKSLHLDLNHQGEMVSCAETRGVRFLGCLVRRSMKESPAVKAVHKLKEKVELFALQK 480
           +F+  SLHLD++HQ EMV C  T G+RFLGCLVRRS +ESPAVKAVHK+KEKVELFA QK
Sbjct: 432 DFLQNSLHLDVHHQEEMVPCQATHGIRFLGCLVRRSEQESPAVKAVHKMKEKVELFAFQK 491

Query: 481 QEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKNSPSLNQISSFRKAGMETDHWYKVLLK 540
           QEAWNAWTVWLGKKWLAHGLKKVKESEIKHLA+ SPSLNQISSFRKAGMETDHWYK LLK
Sbjct: 492 QEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAEQSPSLNQISSFRKAGMETDHWYKALLK 551

Query: 541 IWMQDINAKAAESEETILSNHVVQPSLPLELRDSFHEFQRCVEQYVSSETASTIALLPNY 600
           IWMQ+INA+AAESEETILS HVV+PSLP ELRDSF+EFQRCVE+YVSSETASTIALLPNY
Sbjct: 552 IWMQNINARAAESEETILSKHVVEPSLPQELRDSFYEFQRCVEEYVSSETASTIALLPNY 611

Query: 601 DPSVKSTFITEIIAPVDSIRKRLFRYRLITNKGYPCPSPFLILQDNNQIIDWFSGVSRRW 660
           DPSVK TF+TEIIAPV+SI KRL RYRL+TNKGYPCPSPFLILQD+ QIIDWF GVSRRW
Sbjct: 612 DPSVKPTFVTEIIAPVNSIGKRLRRYRLLTNKGYPCPSPFLILQDDTQIIDWFFGVSRRW 671

Query: 661 LQWYSICSNFSELILICDQVRKSCIRTLAAKHRIHESEIEKKFDSELSRIYSSPEVEQEE 720
            +WY+ CSNFSELILICDQVR+SCIRTLAAKHR+HES+IEKKF+SELS+IYS+P++EQEE
Sbjct: 672 FRWYTNCSNFSELILICDQVRQSCIRTLAAKHRMHESDIEKKFESELSKIYSTPDIEQEE 731

Query: 721 EKRTSDTHVLDHDEALMYGISYSGLCLLSLARMVSQSRPCNCFVIGCLVPAPSVYTLHVM 780
           EK++SDT+ LD+DEALMYGISYSGLCLLSLARMVSQSRPCNCFVIGCL PAPSVYTLHVM
Sbjct: 732 EKKSSDTNGLDNDEALMYGISYSGLCLLSLARMVSQSRPCNCFVIGCLSPAPSVYTLHVM 791

Query: 781 ERQKFPGWNTGFSSSIHPSLNRRRFGLCKQHLKDLYLGHISLQSIDFGAW 792
           ERQKFPGW TGFSSSIHPSLNRRRFGLC++HLKDLYLGHISLQS+DFGAW
Sbjct: 792 ERQKFPGWKTGFSSSIHPSLNRRRFGLCEKHLKDLYLGHISLQSVDFGAW 838

BLAST of Lag0032616 vs. TAIR 10
Match: AT1G74350.1 (Intron maturase, type II family protein )

HSP 1 Score: 875.5 bits (2261), Expect = 3.1e-254
Identity = 447/743 (60.16%), Postives = 554/743 (74.56%), Query Frame = 0

Query: 56  LAINLASLVEESL--VVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACP 115
           LA  LASLVEES   V D  +P++RMEL+RSLE+++KKRVK Q +NGKF DL+ KVIA P
Sbjct: 11  LAGELASLVEESSSHVDDDSKPRSRMELKRSLELRLKKRVKEQCINGKFSDLLKKVIARP 70

Query: 116 TTLQNAYDCIRLNSNVDIASNDHLMSFDSMAEELRNGSFDVNVNTFSI--SSSRKEVLIL 175
            TL++AYDCIRLNSNV I   +  ++FDS+AEEL +G FDV  NTFSI      KEVL+L
Sbjct: 71  ETLRDAYDCIRLNSNVSITERNGSVAFDSIAEELSSGVFDVASNTFSIVARDKTKEVLVL 130

Query: 176 PKLKLKVLQEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLS 235
           P + LKV+QEAIRIVLE VF PHFSKISH CRSGRG ++ALKYI   I   DW FTL L+
Sbjct: 131 PSVALKVVQEAIRIVLEVVFSPHFSKISHSCRSGRGRASALKYINNNISRSDWCFTLSLN 190

Query: 236 KKMDELVMAKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPIL 295
           KK+D  V   +++VME+K+ED +L  ++RS+F+A  LNLEFGGFPKGHGLPQEGVLS +L
Sbjct: 191 KKLDVSVFENLLSVMEEKVEDSSLSILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVL 250

Query: 296 MNIYLNLFDQEFFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNI 355
           MNIYL+ FD EF+R+SM++EA+        D+  SKLRSWFRR       +   E+   +
Sbjct: 251 MNIYLDRFDHEFYRISMRHEALGLDSKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVAL 310

Query: 356 RVYCCRYMDEIFLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLG 415
           RVYCCR+MDEI+ +VSG K V    RSE + F+  SLHLD+  + +   C  T G+R LG
Sbjct: 311 RVYCCRFMDEIYFSVSGPKKVASDIRSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLG 370

Query: 416 CLVRRSMKESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKH 475
            LVR++++ESP VKAVHKLKEKV LFALQK+EAW   TV +GKKWL HGLKKVKESEIK 
Sbjct: 371 TLVRKNVRESPTVKAVHKLKEKVRLFALQKEEAWTLGTVRIGKKWLGHGLKKVKESEIKG 430

Query: 476 LAKNSPSLNQISSFRKAGMETDHWYKVLLKIWMQDI-NAKAAESEETILSNHVVQPSLPL 535
           LA ++ +L+QIS  RKAGMETDHWYK+LL+IWM+D+    A  SEE +LS HVV+P++P 
Sbjct: 431 LADSNSTLSQISCHRKAGMETDHWYKILLRIWMEDVLRTSADRSEEFVLSKHVVEPTVPQ 490

Query: 536 ELRDSFHEFQRCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKRLFRYRLI 595
           ELRD+F++FQ     YVSSETA+  ALLP      +  F  +++AP ++I +RL+RY LI
Sbjct: 491 ELRDAFYKFQNAAAAYVSSETANLEALLPCPQSHDRPVFFGDVVAPTNAIGRRLYRYGLI 550

Query: 596 TNKGYPCPSPFLILQDNNQIIDWFSGVSRRWLQWYSICSNFSEL-ILICDQVRKSCIRTL 655
           T KGY   +  LIL D  QIIDW+SG+ RRW+ WY  CSNF E+  LI +Q+R SCIRTL
Sbjct: 551 TAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSNFDEIKALIDNQIRMSCIRTL 610

Query: 656 AAKHRIHESEIEKKFDSELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGISYSGLCLL 715
           AAK+RIHE+EIEK+ D ELS I S+ ++EQE +    D+   D DE L YG+S SGLCLL
Sbjct: 611 AAKYRIHENEIEKRLDLELSTIPSAEDIEQEIQHEKLDSPAFDRDEHLTYGLSNSGLCLL 670

Query: 716 SLARMVSQSRPCNCFVIGCLVPAPSVYTLHVMERQKFPGWNTGFSSSIHPSLNRRRFGLC 775
           SLAR+VS+SRPCNCFVIGC + AP+VYTLH MERQKFPGW TGFS  I  SLN RR GLC
Sbjct: 671 SLARLVSESRPCNCFVIGCSMAAPAVYTLHAMERQKFPGWKTGFSVCIPSSLNGRRIGLC 730

Query: 776 KQHLKDLYLGHISLQSIDFGAWK 793
           KQHLKDLY+G ISLQ++DFGAW+
Sbjct: 731 KQHLKDLYIGQISLQAVDFGAWR 753

BLAST of Lag0032616 vs. TAIR 10
Match: AT5G04050.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 219.9 bits (559), Expect = 7.1e-57
Identity = 170/603 (28.19%), Postives = 280/603 (46.43%), Query Frame = 0

Query: 67  SLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNAYDCIRL- 126
           SL ++  +  T   ++  LE  V K    QY +GKF  L+   ++ P  L  A   + L 
Sbjct: 31  SLFLNSDQTITEPLVKSELEALVLK----QYSHGKFYSLVKNAVSLPCVLLAACQNLSLS 90

Query: 127 -NSNVDIASN-DHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVLQEAI 186
            NS+ D+A       S + M  E+R G FD+        SS    L+LP LKLKVL EAI
Sbjct: 91  ANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISSS---LVLPNLKLKVLIEAI 150

Query: 187 RIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVMAKII 246
           R+VLE V+   F+  S+G R G G  TA++Y++  ++NP WWF +  +++M E     I+
Sbjct: 151 RMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNVDIL 210

Query: 247 T-VMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLFDQE 306
              + +KI D  L  +I+ +F+ G L +E GG   G G PQE  L  IL+N+Y +  D+E
Sbjct: 211 CGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGLDKE 270

Query: 307 FFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYMDEI 366
              L +K +  N     G + S             GN    P      + +Y  RY+DEI
Sbjct: 271 IQDLRLKMKVKNPRVGTGDEES------------TGNVFFKP------VNIYAVRYLDEI 330

Query: 367 FLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCL-------VR 426
            +  SGSK +T+  +  I++ + + L L ++     +  A +  + FLG         V 
Sbjct: 331 LVITSGSKMLTMDLKKRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVL 390

Query: 427 RSMKESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKN 486
           R  K   AV+A+ K + + ++  L+ + A       LG K   H LKK+K+S        
Sbjct: 391 RPPKSEKAVRAMKKYQRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQS-------- 450

Query: 487 SPSLNQISSFRKAGMETDHWYKVLLKIW----MQDINAKAAE--------SEETILSNHV 546
                  + F+  G E ++  + + + W    MQD      E        +    LS   
Sbjct: 451 -------NGFKFEG-EIENEVRDIFQSWGEEVMQDFMGSLEERWKWHWLLTRGDFLSLRH 510

Query: 547 VQPSLPLELRDSFHEFQRCVEQYVSSETASTIALLPNYDPSVK---------------ST 606
           ++  LP +L D++ EFQ  V+++++   A  +  L + +  V+               + 
Sbjct: 511 IREKLPQDLIDAYDEFQEQVDKHLAPTQAKKV--LEDEERRVEEEEEQRYAERTVEDLTK 570

Query: 607 FITEIIAPVDSIRKRLFRYRLITNKGYPCPSPFLILQDNNQIIDWFSGVSRRWLQWYSIC 632
              ++ AP + +RK +       + G P P   L+  +++ II W++GV R+WL ++  C
Sbjct: 571 LCMKVSAPEELVRKAIKLVGFTNSMGRPRPIIHLVTLEDSDIIKWYAGVGRKWLDFFCCC 590

BLAST of Lag0032616 vs. TAIR 10
Match: AT5G04050.2 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 203.0 bits (515), Expect = 9.0e-52
Identity = 192/751 (25.57%), Postives = 318/751 (42.34%), Query Frame = 0

Query: 67  SLVVDLKRPKTRMELRRSLEIQVKKRVKAQYMNGKFMDLMGKVIACPTTLQNAYDCIRL- 126
           SL ++  +  T   ++  LE  V K    QY +GKF  L+   ++ P  L  A   + L 
Sbjct: 31  SLFLNSDQTITEPLVKSELEALVLK----QYSHGKFYSLVKNAVSLPCVLLAACQNLSLS 90

Query: 127 -NSNVDIASN-DHLMSFDSMAEELRNGSFDVNVNTFSISSSRKEVLILPKLKLKVLQEAI 186
            NS+ D+A       S + M  E+R G FD+        SS    L+LP LKLKVL EAI
Sbjct: 91  ANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISSS---LVLPNLKLKVLIEAI 150

Query: 187 RIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDELVMAKII 246
           R+VLE V+   F+  S+G R G G  TA++Y++  ++NP WWF +  +++M E     I+
Sbjct: 151 RMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNVDIL 210

Query: 247 T-VMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKGHGLPQEGVLSPILMNIYLNLFDQE 306
              + +KI D  L  +I+ +F+ G L +E GG   G G PQE  L  IL+N+Y +  D+E
Sbjct: 211 CGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGLDKE 270

Query: 307 FFRLSMKYEAINKYGNAGRDASQSKLRSWFRRHLKGNDSEYPGEEKDNIRVYCCRYMDEI 366
              L +K +  N     G + S             GN    P      + +Y  RY+DEI
Sbjct: 271 IQDLRLKMKVKNPRVGTGDEES------------TGNVFFKP------VNIYAVRYLDEI 330

Query: 367 FLAVSGSKDVTISFRSEILEFIHKSLHLDLNHQGEMVSCAETRGVRFLGCL-------VR 426
            +  SGSK +T+  +  I++ + + L L ++     +  A +  + FLG         V 
Sbjct: 331 LVITSGSKMLTMDLKKRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVL 390

Query: 427 RSMKESPAVKAVHKLKEKVELFALQKQEAWNAWTVWLGKKWLAHGLKKVKESEIKHLAKN 486
           R  K   AV+A+ K + + ++  L+ + A       LG K   H LKK+K+S        
Sbjct: 391 RPPKSEKAVRAMKKYQRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQS-------- 450

Query: 487 SPSLNQISSFRKAGMETDHWYKVLLKIW----MQDINAKAAE--------SEETILSNHV 546
                  + F+  G E ++  + + + W    MQD      E        +    LS   
Sbjct: 451 -------NGFKFEG-EIENEVRDIFQSWGEEVMQDFMGSLEERWKWHWLLTRGDFLSLRH 510

Query: 547 VQPSLPLELRDSFHEFQRCVEQYVSSETASTIALLPNYDPSVKSTFITEIIAPVDSIRKR 606
           ++  LP +L D++ EFQ  V+++                           +AP  + +  
Sbjct: 511 IREKLPQDLIDAYDEFQEQVDKH---------------------------LAPTQAKK-- 570

Query: 607 LFRYRLITNKGYPCPSPFLILQDNNQIIDWFSGVSRRWLQWYSICSNFSELILICDQVRK 666
                              +L+D  + ++      +R+ +               + + K
Sbjct: 571 -------------------VLEDEERRVE--EEEEQRYAE------------RTVEDLTK 630

Query: 667 SCIRTLAAKHRIHESEIEKKFDS-ELSRIYSSPEVEQEEEKRTSDTHVLDHDEALMYGIS 726
            C++  A +  + ++      D  E +   S  EV+   +K  SD   +D          
Sbjct: 631 LCMKVSAPEELVRKAIKVSDLDGREEAHFPSEREVKMMGDKNLSDPKPVD---------- 665

Query: 727 YSGLCLLSLARMVSQSRPCNCFVIGCLVPAPSVYTLHVMERQKF------PGWNTGFSSS 786
             G   L L R+ S     +C    C      ++ +H+++ +          W  G   +
Sbjct: 691 --GTLSLLLIRLASDEPLHHCAASFCERSDTIMHRVHLLQNRLHINPLDEEKWVPGM-GT 665

Query: 787 IHPSLNRRRFGLCKQHLKDLYLGHISLQSID 788
           IH +LNR+   LC  H+ D+YLG I+LQ +D
Sbjct: 751 IHSALNRKCLPLCSTHISDVYLGKITLQDVD 665

BLAST of Lag0032616 vs. TAIR 10
Match: ATMG00520.1 (Intron maturase, type II family protein )

HSP 1 Score: 85.5 bits (210), Expect = 2.1e-16
Identity = 57/173 (32.95%), Postives = 92/173 (53.18%), Query Frame = 0

Query: 177 KVLQEAIRIVLECVFRPHFSKISHGCRSGRGHSTALKYIRKEIDNPDWWFTLDLSKKMDE 236
           K+++EAIR+VLE ++ P F   SH  RSG+G  + L+ I++E     W+   D+ K    
Sbjct: 15  KIMKEAIRMVLESIYDPEFPDTSH-FRSGQGCHSVLRRIKEEWGISRWFLEFDIRKCFHT 74

Query: 237 LVMAKIITVMEDKIEDPNLFAVIRSIFDAGALNLEFGGFPKG-HGLPQEGVLSPILMNIY 296
           +   ++I +++++I+DP  F  I+ +F AG L     G  +G + +P   +LS +  NIY
Sbjct: 75  IDRHRLIQILKEEIDDPKFFYSIQKVFSAGRL----VGVERGPYSVPHSVLLSALPGNIY 134

Query: 297 LNLFDQEFFRLSMKYEAINKYGNAGRDASQSKLRS-WFRRHLKGNDSEYPGEE 348
           L+  DQE  R+  KYE               ++RS   R   + +D E PGEE
Sbjct: 135 LHKLDQEIGRIRQKYEI----------PIVQRVRSVLLRTGRRIDDQENPGEE 172