Lag0032566 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0032566 vs. NCBI nr
Match: KAA0042649.1 (embryogenesis-associated protein EMB8 [Cucumis melo var. makuwa] >TYK06050.1 embryogenesis-associated protein EMB8 [Cucumis melo var. makuwa])

HSP 1 Score: 773.1 bits (1995), Expect = 1.5e-219
Identity = 378/446 (84.75%), Postives = 401/446 (89.91%), Query Frame = 0

Query: 69  HKNHSRSSSISQAPIPSHRPPMARPFLGSTLPSSPIIALRFSSFIPP-LVTNHRRRSPRS 128
           + NHSRSS IS  PIPSH PPMAR FLGST  SSP+IALR SSF P   +TN R RS R 
Sbjct: 13  NNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRP 72

Query: 129 TTAVVVAMDESSSQKHIQPHPSLEVIGGGRDRFLSAFKDLDQPYKPFPVIGSNRHLETIF 188
           TT  VVAM E  S+K I+PHPSLEVIGGG D+FL AFKDLD PYKPFPVIGSNRHLETIF
Sbjct: 73  TTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIF 132

Query: 189 ASFFRTCPNVRLRRECLRAPDNGTVALDWVSGDDLRLPLDSPVLILLPGLTGGSQDSYVR 248
           ASFFRTCP+V L RECLRA DNGTVALDWV+GDDLRLPLDSPVLILLPGLTGGSQDSYVR
Sbjct: 133 ASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVR 192

Query: 249 HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVG 308
           HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT RYP ANVYAVG
Sbjct: 193 HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVG 252

Query: 309 WSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADEDFRKGFNNIYDKSLAGALRRIF 368
           WSLGGNILVNYLGQD L+CPLSGAVSLCNPFDL+VADEDFRKGFNN+YDKSLA ALR+IF
Sbjct: 253 WSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIF 312

Query: 369 NKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKI 428
           NKHA LF DMP EFNI LAA ARTVRDYDDALTRVSFGFN+VDEYY KSSSS+FIKDVKI
Sbjct: 313 NKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKI 372

Query: 429 PLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGAPWTDPFVI 488
           PL CIQAANDPIAP+R IPREDIKENPNC+LIVTP+GGHLGWVAGPGAPLG PWTDPFVI
Sbjct: 373 PLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVI 432

Query: 489 DFLVHLESRASKASASSADLTAAEML 514
           DFL++LE++ SKASASS DLT AE++
Sbjct: 433 DFLMYLENQKSKASASSTDLTTAEVM 458

BLAST of Lag0032566 vs. NCBI nr
Match: XP_008437445.1 (PREDICTED: LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 [Cucumis melo])

HSP 1 Score: 773.1 bits (1995), Expect = 1.5e-219
Identity = 378/446 (84.75%), Postives = 401/446 (89.91%), Query Frame = 0

Query: 69  HKNHSRSSSISQAPIPSHRPPMARPFLGSTLPSSPIIALRFSSFIPP-LVTNHRRRSPRS 128
           + NHSRSS IS  PIPSH PPMAR FLGST  SSP+IALR SSF P   +TN R RS R 
Sbjct: 6   NNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRP 65

Query: 129 TTAVVVAMDESSSQKHIQPHPSLEVIGGGRDRFLSAFKDLDQPYKPFPVIGSNRHLETIF 188
           TT  VVAM E  S+K I+PHPSLEVIGGG D+FL AFKDLD PYKPFPVIGSNRHLETIF
Sbjct: 66  TTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIF 125

Query: 189 ASFFRTCPNVRLRRECLRAPDNGTVALDWVSGDDLRLPLDSPVLILLPGLTGGSQDSYVR 248
           ASFFRTCP+V L RECLRA DNGTVALDWV+GDDLRLPLDSPVLILLPGLTGGSQDSYVR
Sbjct: 126 ASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVR 185

Query: 249 HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVG 308
           HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT RYP ANVYAVG
Sbjct: 186 HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVG 245

Query: 309 WSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADEDFRKGFNNIYDKSLAGALRRIF 368
           WSLGGNILVNYLGQD L+CPLSGAVSLCNPFDL+VADEDFRKGFNN+YDKSLA ALR+IF
Sbjct: 246 WSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIF 305

Query: 369 NKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKI 428
           NKHA LF DMP EFNI LAA ARTVRDYDDALTRVSFGFN+VDEYY KSSSS+FIKDVKI
Sbjct: 306 NKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKI 365

Query: 429 PLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGAPWTDPFVI 488
           PL CIQAANDPIAP+R IPREDIKENPNC+LIVTP+GGHLGWVAGPGAPLG PWTDPFVI
Sbjct: 366 PLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVI 425

Query: 489 DFLVHLESRASKASASSADLTAAEML 514
           DFL++LE++ SKASASS DLT AE++
Sbjct: 426 DFLMYLENQKSKASASSTDLTTAEVM 451

BLAST of Lag0032566 vs. NCBI nr
Match: XP_022977772.1 (embryogenesis-associated protein EMB8-like [Cucurbita maxima])

HSP 1 Score: 767.7 bits (1981), Expect = 6.4e-218
Identity = 374/434 (86.18%), Postives = 391/434 (90.09%), Query Frame = 0

Query: 80  QAPIPSHRPPMARPFLGSTLPSSPIIALRFSSFIPPLVTNHRRRSPRSTTAVVVAMDESS 139
           Q PIP H PPMAR FLGST  SS +   R SSF P L+TN RRRS R TTA VVAM E  
Sbjct: 18  QTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRRRSLRLTTAAVVAMSEFP 77

Query: 140 SQKHIQPHPSLEVIGGGRDRFLSAFKDLDQPYKPFPVIGSNRHLETIFASFFRTCPNVRL 199
           S+K IQPHPSLEVIGGGRDRFL A KDL+ PYKPFPVIG NRHLETIFASF+R+CP+VR 
Sbjct: 78  SEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRF 137

Query: 200 RRECLRAPDNGTVALDWVSGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWR 259
           RRECLRA DNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR
Sbjct: 138 RRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWR 197

Query: 260 VVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVGWSLGGNILVNYL 319
            VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVT RYP ANVYAVGWSLGGNILVNYL
Sbjct: 198 AVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYL 257

Query: 320 GQDFLSCPLSGAVSLCNPFDLVVADEDFRKGFNNIYDKSLAGALRRIFNKHADLFNDMPG 379
           GQD LSCPLSGAVSLCNPFDLVVADEDFRKGFNN+YDK+LA ALRRIF+KHADLFNDMPG
Sbjct: 258 GQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPG 317

Query: 380 EFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKIPLFCIQAANDPI 439
           EFNIPLAANARTVR+YDDALTRVSFGFNSVDEYY KSSSS+FIKDVKIPL CIQAANDPI
Sbjct: 318 EFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPI 377

Query: 440 APDRAIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGAPWTDPFVIDFLVHLESRASK 499
           APDR +PREDIK NPNCLLIVTPKGGHLGWVAGP APLG PWTDPFVIDFLV+LES+ SK
Sbjct: 378 APDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESK 437

Query: 500 ASASSADLTAAEML 514
           ASASS DLTAAE L
Sbjct: 438 ASASSTDLTAAETL 451

BLAST of Lag0032566 vs. NCBI nr
Match: XP_004143840.3 (embryogenesis-associated protein EMB8 [Cucumis sativus] >KGN49619.2 hypothetical protein Csa_000306 [Cucumis sativus])

HSP 1 Score: 767.3 bits (1980), Expect = 8.3e-218
Identity = 379/465 (81.51%), Postives = 407/465 (87.53%), Query Frame = 0

Query: 51  PISSLPKSEIKNHGVYG-THKNHSRSSSISQAPIPSHRPPMARPFLGSTLPSSPIIALRF 110
           P S     +I++ G+    + NHSRSS IS  PIPSH PPMAR FLGST  SSP+IALR 
Sbjct: 15  PSSDQIDIQIQDDGIIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTPSSSPLIALRL 74

Query: 111 SSFIPP-LVTNHRRRSPRSTTAVVVAMDESSSQKHIQPHPSLEVIGGGRDRFLSAFKDLD 170
           SSF P   +TN R RS R TT  VV M E  S+K I+PHPSLEVIGGG D+FL AFKDLD
Sbjct: 75  SSFTPSFFLTNQRPRSLRLTTDTVVPMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLD 134

Query: 171 QPYKPFPVIGSNRHLETIFASFFRTCPNVRLRRECLRAPDNGTVALDWVSGDDLRLPLDS 230
            PYKPFPVIGSNRHLETIFASFFRTCP+V L RECLRA DNGTVALDWV+GDDLRLPLDS
Sbjct: 135 LPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDS 194

Query: 231 PVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMR 290
           PVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMR
Sbjct: 195 PVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMR 254

Query: 291 EVVAHVTTRYPNANVYAVGWSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADEDFR 350
           EVVAHVT RYP AN+YAVGWSLGGNILVNYLGQD L+CPLSGAVSLCNPFDL+VADEDFR
Sbjct: 255 EVVAHVTERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFR 314

Query: 351 KGFNNIYDKSLAGALRRIFNKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFGFNS 410
           KGFNN+YDKSLAGALR+IFNKHA LF DM GEFNI LAA A TVRDYDDALTRVSFGFN+
Sbjct: 315 KGFNNVYDKSLAGALRKIFNKHAALFTDMTGEFNIRLAAKATTVRDYDDALTRVSFGFNT 374

Query: 411 VDEYYFKSSSSQFIKDVKIPLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGGHLG 470
           VDEYY KSSSS+FIKDVKIPL CIQAANDPIAP+R IPREDI+ENPNC+LIVTPKGGHLG
Sbjct: 375 VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLG 434

Query: 471 WVAGPGAPLGAPWTDPFVIDFLVHLESRASKASASSADLTAAEML 514
           WVAGPGAPLG PWTDPFVIDFL++LE++  KASASS DLT AE++
Sbjct: 435 WVAGPGAPLGFPWTDPFVIDFLMYLENKKFKASASSTDLTMAEVM 479

BLAST of Lag0032566 vs. NCBI nr
Match: XP_022949852.1 (embryogenesis-associated protein EMB8-like [Cucurbita moschata])

HSP 1 Score: 765.0 bits (1974), Expect = 4.1e-217
Identity = 373/434 (85.94%), Postives = 390/434 (89.86%), Query Frame = 0

Query: 80  QAPIPSHRPPMARPFLGSTLPSSPIIALRFSSFIPPLVTNHRRRSPRSTTAVVVAMDESS 139
           Q PIP H PPMAR FLGST  SS +   R SSF P L+ N RRRS R TTA VVAM E  
Sbjct: 18  QTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLINNQRRRSLRLTTAAVVAMSEFP 77

Query: 140 SQKHIQPHPSLEVIGGGRDRFLSAFKDLDQPYKPFPVIGSNRHLETIFASFFRTCPNVRL 199
           S+K IQPHPSLEVIGGG DRFL A KDL+ PYKPFPVIG NRHLETIFASF+R+CP+VR 
Sbjct: 78  SEKPIQPHPSLEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRF 137

Query: 200 RRECLRAPDNGTVALDWVSGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWR 259
           RRECLRA DNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR
Sbjct: 138 RRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWR 197

Query: 260 VVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVGWSLGGNILVNYL 319
            VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVT RYP ANVYAVGWSLGGNILVNYL
Sbjct: 198 AVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYL 257

Query: 320 GQDFLSCPLSGAVSLCNPFDLVVADEDFRKGFNNIYDKSLAGALRRIFNKHADLFNDMPG 379
           GQD LSCPLSGAVSLCNPFDLVVADEDFRKGFNN+YDK+LA ALRRIF+KHADLFNDMPG
Sbjct: 258 GQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPG 317

Query: 380 EFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKIPLFCIQAANDPI 439
           EFNIPLAANARTVR+YDDALTRVSFGFNSVDEYY KSSSS+FIKDVKIPL CIQAANDPI
Sbjct: 318 EFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPI 377

Query: 440 APDRAIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGAPWTDPFVIDFLVHLESRASK 499
           APDR +PREDIKENPNCLLIVTPKGGHLGWVAGP APLG PWTDPFVIDFLV+LES+ SK
Sbjct: 378 APDRGVPREDIKENPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESK 437

Query: 500 ASASSADLTAAEML 514
           ASASS DLTAAE L
Sbjct: 438 ASASSTDLTAAETL 451

BLAST of Lag0032566 vs. ExPASy Swiss-Prot
Match: Q40863 (Embryogenesis-associated protein EMB8 OS=Picea glauca OX=3330 GN=EMB8 PE=2 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 6.2e-131
Identity = 229/352 (65.06%), Postives = 269/352 (76.42%), Query Frame = 0

Query: 151 EVIGGGRDRFLSAFKDLDQPYKPFPVIGSNRHLETIFASFFRTCPNVRLRRECLRAPDNG 210
           E+IG  R+  +S    L +PY+ FP +G NRH+ETIFASFFR+ P ++ RRECLR  D G
Sbjct: 69  EIIGSDRE-LMSKLTTLGRPYRHFPFMG-NRHVETIFASFFRSWPVIKSRRECLRMEDGG 128

Query: 211 TVALDW-VSGDDL-----RLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFN 270
           TV LDW + G+D       LP++SPVLILLPGLTGGS DSYV+HML+RAR  GW  VVFN
Sbjct: 129 TVELDWPLEGEDAELWNGELPVNSPVLILLPGLTGGSDDSYVKHMLLRARKHGWHSVVFN 188

Query: 271 SRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVGWSLGGNILVNYLGQDFL 330
           SRGC +SPVTTPQFYSASF  D+ +VV HV  R+  +N+YAVGWSLG NILV YLG+   
Sbjct: 189 SRGCADSPVTTPQFYSASFTKDLCQVVKHVAVRFSESNIYAVGWSLGANILVRYLGEVAG 248

Query: 331 SCPLSGAVSLCNPFDLVVADEDFRK--GFNNIYDKSLAGALRRIFNKHADLFNDMPGEFN 390
           +CPLSGAVSLCNPF+LV+ADEDF K  GFNN+YDK+LA  LR+IF KH  LF  + GE+N
Sbjct: 249 NCPLSGAVSLCNPFNLVIADEDFHKGLGFNNVYDKALARGLRQIFPKHTRLFEGIEGEYN 308

Query: 391 IPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKIPLFCIQAANDPIAPD 450
           IP  A AR+VRD+D  LTRVSFGF SV +YY  SSSS  IK V+  L CIQA+NDPIAP 
Sbjct: 309 IPTVAKARSVRDFDGGLTRVSFGFQSVGDYYSNSSSSLSIKYVQTSLLCIQASNDPIAPS 368

Query: 451 RAIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGAPWTDPFVIDFLVHLE 495
           R IP EDIKENPNCLL+VTP GGHLGWVAG  AP GAPWTDP V+++L  LE
Sbjct: 369 RGIPWEDIKENPNCLLVVTPNGGHLGWVAGDDAPFGAPWTDPLVMEYLEVLE 418

BLAST of Lag0032566 vs. ExPASy Swiss-Prot
Match: Q0VC00 (Phospholipase ABHD3 OS=Bos taurus OX=9913 GN=ABHD3 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 2.8e-51
Identity = 100/293 (34.13%), Postives = 167/293 (57.00%), Query Frame = 0

Query: 184 ETIFASFFRTCPNVRLRRECLRAPDNGTVALDWVSGDDLRLPLDS---PVLILLPGLTGG 243
           +T+   F  + P V+ R E ++  D G ++LDW   D+ +  +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPLVQYRNELIKTADGGQISLDWFDNDNSKHYMDASTRPTVLLLPGLTGT 151

Query: 244 SQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPN 303
           S++SY+ HM+  + + G+R VVFN+RG     + TP+ Y  S   D+  V+ HV + YP+
Sbjct: 152 SKESYILHMIHLSEELGYRYVVFNNRGVAGENLLTPRTYCCSNTEDLETVIHHVHSLYPS 211

Query: 304 ANVYAVGWSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADEDFRKGFN-NIYDKSL 363
           A   A G S+GG +L+NYLG+     PL  A +    ++     E   K  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGPKTPLKAAATFSVGWNTFACSESLEKPLNWLLFNYYL 271

Query: 364 AGALRRIFNKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSS 423
              L+   NKH  +F     + ++     A+++R++D   T V FG+ ++D+YY  +S +
Sbjct: 272 TTCLQSSVNKHRHMF---VKQIDVDHVMKAKSIREFDKRFTSVMFGYRTIDDYYTDASPN 331

Query: 424 QFIKDVKIPLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGGHLGWVAG 473
           + +K V IP+ C+ + +D  +P  AIP E  K+NPN  L++T  GGH+G++ G
Sbjct: 332 RRLKSVGIPVLCLNSVDDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of Lag0032566 vs. ExPASy Swiss-Prot
Match: Q8WU67 (Phospholipase ABHD3 OS=Homo sapiens OX=9606 GN=ABHD3 PE=2 SV=2)

HSP 1 Score: 203.0 bits (515), Expect = 8.2e-51
Identity = 100/293 (34.13%), Postives = 165/293 (56.31%), Query Frame = 0

Query: 184 ETIFASFFRTCPNVRLRRECLRAPDNGTVALDWVSGDDLRLPLDS---PVLILLPGLTGG 243
           +T+   F  + P V+ R E ++  D G ++LDW   D+    +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPPVQYRNELIKTADGGQISLDWFDNDNSTCYMDASTRPTILLLPGLTGT 151

Query: 244 SQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPN 303
           S++SY+ HM+  + + G+R VVFN+RG     + TP+ Y  +   D+  V+ HV + YP+
Sbjct: 152 SKESYILHMIHLSEELGYRCVVFNNRGVAGENLLTPRTYCCANTEDLETVIHHVHSLYPS 211

Query: 304 ANVYAVGWSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADEDFRKGFN-NIYDKSL 363
           A   A G S+GG +L+NYLG+     PL  A +    ++     E   K  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGSKTPLMAAATFSVGWNTFACSESLEKPLNWLLFNYYL 271

Query: 364 AGALRRIFNKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSS 423
              L+   NKH  +F     + ++     A+++R++D   T V FG+ ++D+YY  +S S
Sbjct: 272 TTCLQSSVNKHRHMF---VKQVDMDHVMKAKSIREFDKRFTSVMFGYQTIDDYYTDASPS 331

Query: 424 QFIKDVKIPLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGGHLGWVAG 473
             +K V IP+ C+ + +D  +P  AIP E  K+NPN  L++T  GGH+G++ G
Sbjct: 332 PRLKSVGIPVLCLNSVDDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of Lag0032566 vs. ExPASy Swiss-Prot
Match: Q91ZH7 (Phospholipase ABHD3 OS=Mus musculus OX=10090 GN=Abhd3 PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.1e-50
Identity = 108/334 (32.34%), Postives = 179/334 (53.59%), Query Frame = 0

Query: 144 IQPHPSLEVIGGGRDRFLSAF-KDLDQPYKPFPVIGSNRHLETIFASFFRTCPNVRLRRE 203
           I   P L + G    RFL      + + Y P      +R  +T+   F  + P V+ R E
Sbjct: 52  IAKKPQLVIGGESFSRFLQDHCPVVTETYYPTVWCWESRG-QTLLRPFITSKPPVQYRNE 111

Query: 204 CLRAPDNGTVALDWVSGDDLRLPLDS---PVLILLPGLTGGSQDSYVRHMLVRARDRGWR 263
            ++  D G ++LDW   ++    +D+   P ++LLPGLTG S++SY+ HM+  + + G+R
Sbjct: 112 LIKTADGGQISLDWFDNNNSAYYVDASTRPTILLLPGLTGTSKESYILHMIHLSEELGYR 171

Query: 264 VVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVGWSLGGNILVNYL 323
            VVFN+RG     + TP+ Y  +   D+  VV HV + YP A   A G S+GG +L+NYL
Sbjct: 172 CVVFNNRGVAGESLLTPRTYCCANTEDLEAVVHHVHSLYPGAPFLAAGVSMGGMLLLNYL 231

Query: 324 GQDFLSCPLSGAVSLCNPFDLVVADEDFRKGFN-NIYDKSLAGALRRIFNKHADLFNDMP 383
           G+     PL  A +    ++     E   +  N  +++  L   L+    KH  +F +  
Sbjct: 232 GKIGSKTPLMAAATFSVGWNTFACSESLERPLNWLLFNYYLTTCLQSSVKKHRHMFVE-- 291

Query: 384 GEFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKIPLFCIQAANDP 443
            + ++     A+++R++D   T V FG+ ++D+YY  +S ++ +K V IP+ C+ A +D 
Sbjct: 292 -QIDMDQVMKAKSIREFDKRFTAVMFGYRTLDDYYTDASPNRRLKSVGIPVLCLNATDDV 351

Query: 444 IAPDRAIPREDIKENPNCLLIVTPKGGHLGWVAG 473
            +P  AIP E  K+NPN  L++T  GGH+G++ G
Sbjct: 352 FSPSHAIPIETAKQNPNVALVLTAYGGHIGFLEG 381

BLAST of Lag0032566 vs. ExPASy Swiss-Prot
Match: Q54H38 (Abhydrolase domain-containing protein OS=Dictyostelium discoideum OX=44689 GN=abhd PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.5e-47
Identity = 112/317 (35.33%), Postives = 176/317 (55.52%), Query Frame = 0

Query: 180 NRHLETIFASFFRTCPNVRLRRECLRAP-DNGTVALDWVSGDDLRLPLDSPVLILLPGLT 239
           N H    + S+     N++ RRE L  P D GT++LD+    + +   D+P +++  GLT
Sbjct: 69  NSHFMNYYGSYKIPKLNLKTRREILVNPIDGGTISLDFFELGEFK--EDTPTIVINHGLT 128

Query: 240 GGSQDSYVRHMLVRA-RDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT-T 299
           GGS + YV++   RA +++G+R VVFN RGC  +P+T  + YSA  L D++ V  ++T T
Sbjct: 129 GGSHERYVQYFAQRAYKEKGFRSVVFNYRGCAGNPITADRAYSAVQLDDIKFVTEYLTKT 188

Query: 300 RYPNANV-YAVGWSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADEDFRKGF--NN 359
             P     + VG+SLG  ILVNY+       P    VS+ NP ++V   ++    +  N 
Sbjct: 189 ALPLVKKWFLVGFSLGSAILVNYMADAGKDSPYLAHVSISNPMNMVECTKNLSSTYINNL 248

Query: 360 IYDKSLAGALRRIFNKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFGFNSVDEYY 419
           IY+K LA  L+R+F K     +    +  I     A+T+ D+DD +T   FGF +  +YY
Sbjct: 249 IYNKGLANNLKRLFRKFDGRLDKYATKEQI---MAAQTIADFDDLITSKMFGFETAHDYY 308

Query: 420 FKSSSSQFIKDVKIPLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGGHLGWVAGP 479
             +SSS+ I+++  P+  I A +DPIAP    P +D K NPN +L V+  GGHLG+++  
Sbjct: 309 LAASSSKSIRNLVKPILFINAIDDPIAPTSGFPWKDFKSNPNTILCVSRWGGHLGFISYE 368

Query: 480 GAPLGAPWTDPFVIDFL 491
                  W+D   +++L
Sbjct: 369 D---HMSWSDKAAVEYL 377

BLAST of Lag0032566 vs. ExPASy TrEMBL
Match: A0A5A7TMR0 (Embryogenesis-associated protein EMB8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001510 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 7.4e-220
Identity = 378/446 (84.75%), Postives = 401/446 (89.91%), Query Frame = 0

Query: 69  HKNHSRSSSISQAPIPSHRPPMARPFLGSTLPSSPIIALRFSSFIPP-LVTNHRRRSPRS 128
           + NHSRSS IS  PIPSH PPMAR FLGST  SSP+IALR SSF P   +TN R RS R 
Sbjct: 13  NNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRP 72

Query: 129 TTAVVVAMDESSSQKHIQPHPSLEVIGGGRDRFLSAFKDLDQPYKPFPVIGSNRHLETIF 188
           TT  VVAM E  S+K I+PHPSLEVIGGG D+FL AFKDLD PYKPFPVIGSNRHLETIF
Sbjct: 73  TTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIF 132

Query: 189 ASFFRTCPNVRLRRECLRAPDNGTVALDWVSGDDLRLPLDSPVLILLPGLTGGSQDSYVR 248
           ASFFRTCP+V L RECLRA DNGTVALDWV+GDDLRLPLDSPVLILLPGLTGGSQDSYVR
Sbjct: 133 ASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVR 192

Query: 249 HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVG 308
           HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT RYP ANVYAVG
Sbjct: 193 HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVG 252

Query: 309 WSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADEDFRKGFNNIYDKSLAGALRRIF 368
           WSLGGNILVNYLGQD L+CPLSGAVSLCNPFDL+VADEDFRKGFNN+YDKSLA ALR+IF
Sbjct: 253 WSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIF 312

Query: 369 NKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKI 428
           NKHA LF DMP EFNI LAA ARTVRDYDDALTRVSFGFN+VDEYY KSSSS+FIKDVKI
Sbjct: 313 NKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKI 372

Query: 429 PLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGAPWTDPFVI 488
           PL CIQAANDPIAP+R IPREDIKENPNC+LIVTP+GGHLGWVAGPGAPLG PWTDPFVI
Sbjct: 373 PLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVI 432

Query: 489 DFLVHLESRASKASASSADLTAAEML 514
           DFL++LE++ SKASASS DLT AE++
Sbjct: 433 DFLMYLENQKSKASASSTDLTTAEVM 458

BLAST of Lag0032566 vs. ExPASy TrEMBL
Match: A0A1S3AU57 (LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 OS=Cucumis melo OX=3656 GN=LOC103482859 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 7.4e-220
Identity = 378/446 (84.75%), Postives = 401/446 (89.91%), Query Frame = 0

Query: 69  HKNHSRSSSISQAPIPSHRPPMARPFLGSTLPSSPIIALRFSSFIPP-LVTNHRRRSPRS 128
           + NHSRSS IS  PIPSH PPMAR FLGST  SSP+IALR SSF P   +TN R RS R 
Sbjct: 6   NNNHSRSSPISNIPIPSHSPPMARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRP 65

Query: 129 TTAVVVAMDESSSQKHIQPHPSLEVIGGGRDRFLSAFKDLDQPYKPFPVIGSNRHLETIF 188
           TT  VVAM E  S+K I+PHPSLEVIGGG D+FL AFKDLD PYKPFPVIGSNRHLETIF
Sbjct: 66  TTGSVVAMAEVPSEKRIKPHPSLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIF 125

Query: 189 ASFFRTCPNVRLRRECLRAPDNGTVALDWVSGDDLRLPLDSPVLILLPGLTGGSQDSYVR 248
           ASFFRTCP+V L RECLRA DNGTVALDWV+GDDLRLPLDSPVLILLPGLTGGSQDSYVR
Sbjct: 126 ASFFRTCPSVNLHRECLRAADNGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVR 185

Query: 249 HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVG 308
           HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVT RYP ANVYAVG
Sbjct: 186 HMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVG 245

Query: 309 WSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADEDFRKGFNNIYDKSLAGALRRIF 368
           WSLGGNILVNYLGQD L+CPLSGAVSLCNPFDL+VADEDFRKGFNN+YDKSLA ALR+IF
Sbjct: 246 WSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIF 305

Query: 369 NKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKI 428
           NKHA LF DMP EFNI LAA ARTVRDYDDALTRVSFGFN+VDEYY KSSSS+FIKDVKI
Sbjct: 306 NKHAALFTDMPSEFNIRLAAKARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKI 365

Query: 429 PLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGAPWTDPFVI 488
           PL CIQAANDPIAP+R IPREDIKENPNC+LIVTP+GGHLGWVAGPGAPLG PWTDPFVI
Sbjct: 366 PLLCIQAANDPIAPNRGIPREDIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVI 425

Query: 489 DFLVHLESRASKASASSADLTAAEML 514
           DFL++LE++ SKASASS DLT AE++
Sbjct: 426 DFLMYLENQKSKASASSTDLTTAEVM 451

BLAST of Lag0032566 vs. ExPASy TrEMBL
Match: A0A6J1IJD9 (embryogenesis-associated protein EMB8-like OS=Cucurbita maxima OX=3661 GN=LOC111477973 PE=4 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 3.1e-218
Identity = 374/434 (86.18%), Postives = 391/434 (90.09%), Query Frame = 0

Query: 80  QAPIPSHRPPMARPFLGSTLPSSPIIALRFSSFIPPLVTNHRRRSPRSTTAVVVAMDESS 139
           Q PIP H PPMAR FLGST  SS +   R SSF P L+TN RRRS R TTA VVAM E  
Sbjct: 18  QTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLITNQRRRSLRLTTAAVVAMSEFP 77

Query: 140 SQKHIQPHPSLEVIGGGRDRFLSAFKDLDQPYKPFPVIGSNRHLETIFASFFRTCPNVRL 199
           S+K IQPHPSLEVIGGGRDRFL A KDL+ PYKPFPVIG NRHLETIFASF+R+CP+VR 
Sbjct: 78  SEKPIQPHPSLEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRF 137

Query: 200 RRECLRAPDNGTVALDWVSGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWR 259
           RRECLRA DNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR
Sbjct: 138 RRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWR 197

Query: 260 VVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVGWSLGGNILVNYL 319
            VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVT RYP ANVYAVGWSLGGNILVNYL
Sbjct: 198 AVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYL 257

Query: 320 GQDFLSCPLSGAVSLCNPFDLVVADEDFRKGFNNIYDKSLAGALRRIFNKHADLFNDMPG 379
           GQD LSCPLSGAVSLCNPFDLVVADEDFRKGFNN+YDK+LA ALRRIF+KHADLFNDMPG
Sbjct: 258 GQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPG 317

Query: 380 EFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKIPLFCIQAANDPI 439
           EFNIPLAANARTVR+YDDALTRVSFGFNSVDEYY KSSSS+FIKDVKIPL CIQAANDPI
Sbjct: 318 EFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPI 377

Query: 440 APDRAIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGAPWTDPFVIDFLVHLESRASK 499
           APDR +PREDIK NPNCLLIVTPKGGHLGWVAGP APLG PWTDPFVIDFLV+LES+ SK
Sbjct: 378 APDRGVPREDIKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESK 437

Query: 500 ASASSADLTAAEML 514
           ASASS DLTAAE L
Sbjct: 438 ASASSTDLTAAETL 451

BLAST of Lag0032566 vs. ExPASy TrEMBL
Match: A0A0A0KNN2 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G148530 PE=4 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 4.0e-218
Identity = 379/465 (81.51%), Postives = 407/465 (87.53%), Query Frame = 0

Query: 51  PISSLPKSEIKNHGVYG-THKNHSRSSSISQAPIPSHRPPMARPFLGSTLPSSPIIALRF 110
           P S     +I++ G+    + NHSRSS IS  PIPSH PPMAR FLGST  SSP+IALR 
Sbjct: 15  PSSDQIDIQIQDDGIIRILNNNHSRSSPISNIPIPSHSPPMARAFLGSTPSSSPLIALRL 74

Query: 111 SSFIPP-LVTNHRRRSPRSTTAVVVAMDESSSQKHIQPHPSLEVIGGGRDRFLSAFKDLD 170
           SSF P   +TN R RS R TT  VV M E  S+K I+PHPSLEVIGGG D+FL AFKDLD
Sbjct: 75  SSFTPSFFLTNQRPRSLRLTTDTVVPMAELPSEKRIKPHPSLEVIGGGCDQFLPAFKDLD 134

Query: 171 QPYKPFPVIGSNRHLETIFASFFRTCPNVRLRRECLRAPDNGTVALDWVSGDDLRLPLDS 230
            PYKPFPVIGSNRHLETIFASFFRTCP+V L RECLRA DNGTVALDWV+GDDLRLPLDS
Sbjct: 135 LPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAADNGTVALDWVAGDDLRLPLDS 194

Query: 231 PVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMR 290
           PVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMR
Sbjct: 195 PVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMR 254

Query: 291 EVVAHVTTRYPNANVYAVGWSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADEDFR 350
           EVVAHVT RYP AN+YAVGWSLGGNILVNYLGQD L+CPLSGAVSLCNPFDL+VADEDFR
Sbjct: 255 EVVAHVTERYPKANLYAVGWSLGGNILVNYLGQDSLTCPLSGAVSLCNPFDLIVADEDFR 314

Query: 351 KGFNNIYDKSLAGALRRIFNKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFGFNS 410
           KGFNN+YDKSLAGALR+IFNKHA LF DM GEFNI LAA A TVRDYDDALTRVSFGFN+
Sbjct: 315 KGFNNVYDKSLAGALRKIFNKHAALFTDMTGEFNIRLAAQATTVRDYDDALTRVSFGFNT 374

Query: 411 VDEYYFKSSSSQFIKDVKIPLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGGHLG 470
           VDEYY KSSSS+FIKDVKIPL CIQAANDPIAP+R IPREDI+ENPNC+LIVTPKGGHLG
Sbjct: 375 VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPREDIEENPNCMLIVTPKGGHLG 434

Query: 471 WVAGPGAPLGAPWTDPFVIDFLVHLESRASKASASSADLTAAEML 514
           WVAGPGAPLG PWTDPFVIDFL++LE++  KASASS DLT AE++
Sbjct: 435 WVAGPGAPLGFPWTDPFVIDFLMYLENKKFKASASSTDLTMAEVM 479

BLAST of Lag0032566 vs. ExPASy TrEMBL
Match: A0A6J1GD89 (embryogenesis-associated protein EMB8-like OS=Cucurbita moschata OX=3662 GN=LOC111453123 PE=4 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 2.0e-217
Identity = 373/434 (85.94%), Postives = 390/434 (89.86%), Query Frame = 0

Query: 80  QAPIPSHRPPMARPFLGSTLPSSPIIALRFSSFIPPLVTNHRRRSPRSTTAVVVAMDESS 139
           Q PIP H PPMAR FLGST  SS +   R SSF P L+ N RRRS R TTA VVAM E  
Sbjct: 18  QTPIPFHFPPMARAFLGSTPSSSLLTTSRLSSFTPSLINNQRRRSLRLTTAAVVAMSEFP 77

Query: 140 SQKHIQPHPSLEVIGGGRDRFLSAFKDLDQPYKPFPVIGSNRHLETIFASFFRTCPNVRL 199
           S+K IQPHPSLEVIGGG DRFL A KDL+ PYKPFPVIG NRHLETIFASF+R+CP+VR 
Sbjct: 78  SEKPIQPHPSLEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRF 137

Query: 200 RRECLRAPDNGTVALDWVSGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWR 259
           RRECLRA DNGTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR
Sbjct: 138 RRECLRAADNGTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWR 197

Query: 260 VVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVGWSLGGNILVNYL 319
            VVFNSRGCGNSPVTTPQFYSASFLGDMREVV HVT RYP ANVYAVGWSLGGNILVNYL
Sbjct: 198 AVVFNSRGCGNSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYL 257

Query: 320 GQDFLSCPLSGAVSLCNPFDLVVADEDFRKGFNNIYDKSLAGALRRIFNKHADLFNDMPG 379
           GQD LSCPLSGAVSLCNPFDLVVADEDFRKGFNN+YDK+LA ALRRIF+KHADLFNDMPG
Sbjct: 258 GQDSLSCPLSGAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPG 317

Query: 380 EFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKIPLFCIQAANDPI 439
           EFNIPLAANARTVR+YDDALTRVSFGFNSVDEYY KSSSS+FIKDVKIPL CIQAANDPI
Sbjct: 318 EFNIPLAANARTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPI 377

Query: 440 APDRAIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGAPWTDPFVIDFLVHLESRASK 499
           APDR +PREDIKENPNCLLIVTPKGGHLGWVAGP APLG PWTDPFVIDFLV+LES+ SK
Sbjct: 378 APDRGVPREDIKENPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESK 437

Query: 500 ASASSADLTAAEML 514
           ASASS DLTAAE L
Sbjct: 438 ASASSTDLTAAETL 451

BLAST of Lag0032566 vs. TAIR 10
Match: AT3G50790.1 (esterase/lipase/thioesterase family protein )

HSP 1 Score: 566.2 bits (1458), Expect = 2.6e-161
Identity = 271/389 (69.67%), Postives = 318/389 (81.75%), Query Frame = 0

Query: 109 FSSFIPPLVTNHRRRSPRSTTAVVVAMDESSSQKHIQPHPSLEVIGGGRDRFLSAFKD-L 168
           F++  PPL+++ R    R  +    A  E S   H   H SLEVIGGG DRFL + KD L
Sbjct: 6   FATSSPPLISSVRTLR-RHFSVFAAANPEMSRPSH---HSSLEVIGGGSDRFLPSLKDSL 65

Query: 169 DQPYKPFPVIGSNRHLETIFASFFRTCPNVRLRRECLRAPDNGTVALDWVSGDDLRLPLD 228
            +PY  FP+IG NRH+ETI+ASFFR+ P VRLRRECLR  DNG+VALDWV+G+D   P D
Sbjct: 66  AKPYNAFPLIGFNRHVETIYASFFRSVPFVRLRRECLRTKDNGSVALDWVAGEDRHFPPD 125

Query: 229 SPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDM 288
           SP+LILLPGLTGGSQDSYVRHML+RA+ + WR VVFNSRGCG+SPVTTPQFYSASFLGD+
Sbjct: 126 SPILILLPGLTGGSQDSYVRHMLLRAQSKKWRCVVFNSRGCGDSPVTTPQFYSASFLGDI 185

Query: 289 REVVAHVTTRYPNANVYAVGWSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADEDF 348
            EV+ HV  ++P AN+YA GWSLGGNILVNYLGQ+  +CPL+ AVSLCNPFDLV+ADEDF
Sbjct: 186 GEVIDHVVDKFPKANLYAAGWSLGGNILVNYLGQESHNCPLTAAVSLCNPFDLVIADEDF 245

Query: 349 RKGFNNIYDKSLAGALRRIFNKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFGFN 408
            KGFNN+YDK+L+ +LRRIF+KH+ LF D+ GEFNIPLAANA TVRD+DD LTRVSFGF 
Sbjct: 246 HKGFNNVYDKALSKSLRRIFSKHSLLFEDIGGEFNIPLAANAETVRDFDDGLTRVSFGFK 305

Query: 409 SVDEYYFKSSSSQFIKDVKIPLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGGHL 468
           SVDEYY KSSSS+ IK V+IPL CIQAANDPIAP+R IPR+DIK NPNC+LIVTP+GGHL
Sbjct: 306 SVDEYYSKSSSSKAIKHVRIPLLCIQAANDPIAPERGIPRDDIKANPNCVLIVTPRGGHL 365

Query: 469 GWVAGPGAPLGAPWTDPFVIDFLVHLESR 497
           GWVAG  AP GAPWTD  V++FL H+E+R
Sbjct: 366 GWVAGEEAPNGAPWTDQVVMEFLQHVENR 390

BLAST of Lag0032566 vs. TAIR 10
Match: AT5G49950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 219.9 bits (559), Expect = 4.6e-57
Identity = 110/307 (35.83%), Postives = 176/307 (57.33%), Query Frame = 0

Query: 179 SNRHLETIFASFFRTCPNVRLRRECLRAPDNGTVALDWVSGDDLRLPLD----------- 238
           S+ HL+T F + F   P    +R   +A D GT+ALDW+   D+   +            
Sbjct: 87  SSPHLQTAFLTLFGKSPPFSYKRILYQATDGGTIALDWLMHSDVVEGISQVVNASNPGTD 146

Query: 239 -SPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGD 298
            +P+ I++PGLT  S  +Y++H+  R    GW VVV N RG G   +T+   Y+A +  D
Sbjct: 147 RTPIAIIVPGLTSDSSAAYIKHIAFRLAKEGWNVVVQNHRGLGGISLTSDCVYTAGWTED 206

Query: 299 MREVVAHVTTRYPNANVYAVGWSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADED 358
           +R+V+AH+ +++P A ++AVG S+G N+LV YLG+D  + PL GA ++C+P+DL++ D  
Sbjct: 207 LRKVIAHIHSQFPEAPLFAVGTSIGANVLVKYLGEDGPNTPLIGATAVCSPWDLLICDRF 266

Query: 359 F-RKGFNNIYDKSLAGALRRIFNKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFG 418
             RK    +YD+ L   L+     H  + + +     I     +R+VR++D+  TR+   
Sbjct: 267 INRKLVQKVYDRMLTIGLQGYAQLHHSIISRIADWEGI---KKSRSVREFDNYATRLVAK 326

Query: 419 FNSVDEYYFKSSSSQFIKDVKIPLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGG 473
           F + D YY +SSSSQ++++V +PL CI A +DP+    AIP ++ + N N +L  T  GG
Sbjct: 327 FETTDTYYRRSSSSQYVENVAVPLLCISALDDPVCTREAIPWDECRANKNIVLATTTHGG 386

BLAST of Lag0032566 vs. TAIR 10
Match: AT1G34340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 206.5 bits (524), Expect = 5.3e-53
Identity = 115/349 (32.95%), Postives = 181/349 (51.86%), Query Frame = 0

Query: 182 HLETIFASFFRTCPNVRLRRECLRAPDNGTVALDWVSGDDLRLPLD-------------- 241
           H++T F +F    P     R+     D GT+ALDW++  D+   LD              
Sbjct: 96  HIQTCFLNFHGLPPVFTYTRQLFLTSDGGTIALDWLTNSDV---LDGSLHNKSEITKEDT 155

Query: 242 SPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDM 301
           +P+ +++PGLT  S  +Y++H+       GW VV+ N RG G   VT+  FY+A +  D+
Sbjct: 156 TPIAVVIPGLTSDSSSAYLKHLAYDTAKTGWNVVISNHRGLGGVSVTSDCFYNAGWTDDI 215

Query: 302 REVVAHVTTRYPNANVYAVGWSLGGNILVNYLGQDFLSCPLSGAVSLCNPFDLVVADEDF 361
           R V+ ++  +YP A ++A+G S+G N+LV YLG++    PL GAV++C+P+DL++ D   
Sbjct: 216 RVVLDYLQHKYPRAPLFAIGTSIGANVLVKYLGEEGEKTPLRGAVAICSPWDLLIGDRFI 275

Query: 362 -RKGFNNIYDKSLAGALRRIFNKHADLFNDMPGEFNIPLAANARTVRDYDDALTRVSFGF 421
            R     +YDK+L   L+     H   F  +     I     +R++RD+D+  T +   F
Sbjct: 276 CRTLKQKLYDKALTIGLQGYAQLHEPQFLRLANWEGI---KKSRSIRDFDNHATCLVGKF 335

Query: 422 NSVDEYYFKSSSSQFIKDVKIPLFCIQAANDPIAPDRAIPREDIKENPNCLLIVTPKGGH 481
            +VD YY KSSS+Q++ +V +PL CI A +DP+    AIP ++ + N N +L  T  GGH
Sbjct: 336 ETVDTYYRKSSSTQYVGNVAVPLLCISALDDPLCTKEAIPWDECRANKNIVLATTNHGGH 395

Query: 482 LG-----------WVAGPGAPLGAPWTDPFVIDFLVHLESRASKASASS 505
           L            WV      LG     P+     +H++    K S+ S
Sbjct: 396 LAFFEGLTGSSLWWVRATNEFLGVLSCSPY-----MHIQKIVDKRSSGS 433

BLAST of Lag0032566 vs. TAIR 10
Match: AT2G03140.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 155.6 bits (392), Expect = 1.1e-37
Identity = 95/298 (31.88%), Postives = 151/298 (50.67%), Query Frame = 0

Query: 200 RRECLRAPDNGTVALDWVSGDDLR--LPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRG 259
           +R C+   D G V+LDW +  D+R    LD+ V + +PG   GS +  VR  +  A  RG
Sbjct: 171 QRVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEALRRG 230

Query: 260 WRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVGWSLGGNILVN 319
              VV N RGC  SP+TTP+ ++A    D+   +  ++   P   + AVG   G N+L  
Sbjct: 231 VFPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANMLTK 290

Query: 320 YLGQDFLSCPLSGAVSLCNPFDLVVADEDFRKG-FNNIYDKSLAGALRRIFNKHADLFND 379
           YL +     PL+ AV + NPFDL   +E  R   ++   D+ L   L  I   + +LF  
Sbjct: 291 YLAEAGERTPLTAAVCIDNPFDL---EEITRTSPYSTSLDQQLTRGLVEILLANKELFQG 350

Query: 380 MPGEFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKIPLFCIQAAN 439
               F++  A  +++VR++D AL+ V++G  S++++Y   ++ + I +VK+PL  IQ  N
Sbjct: 351 RAKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ--N 410

Query: 440 DPIAPDRAIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGAPWTDPFVIDFLVHLE 495
           D + P   IPR  I ENP   L++     +L      G  +   W      ++L  +E
Sbjct: 411 DDVVPPYTIPRSSIAENPFTSLLLCSSSPNL----IDGRTVAVSWCQDLASEWLTAVE 458

BLAST of Lag0032566 vs. TAIR 10
Match: AT2G03140.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 155.6 bits (392), Expect = 1.1e-37
Identity = 95/298 (31.88%), Postives = 151/298 (50.67%), Query Frame = 0

Query: 200 RRECLRAPDNGTVALDWVSGDDLR--LPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRG 259
           +R C+   D G V+LDW +  D+R    LD+ V + +PG   GS +  VR  +  A  RG
Sbjct: 171 QRVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEALRRG 230

Query: 260 WRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVAHVTTRYPNANVYAVGWSLGGNILVN 319
              VV N RGC  SP+TTP+ ++A    D+   +  ++   P   + AVG   G N+L  
Sbjct: 231 VFPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANMLTK 290

Query: 320 YLGQDFLSCPLSGAVSLCNPFDLVVADEDFRKG-FNNIYDKSLAGALRRIFNKHADLFND 379
           YL +     PL+ AV + NPFDL   +E  R   ++   D+ L   L  I   + +LF  
Sbjct: 291 YLAEAGERTPLTAAVCIDNPFDL---EEITRTSPYSTSLDQQLTRGLVEILLANKELFQG 350

Query: 380 MPGEFNIPLAANARTVRDYDDALTRVSFGFNSVDEYYFKSSSSQFIKDVKIPLFCIQAAN 439
               F++  A  +++VR++D AL+ V++G  S++++Y   ++ + I +VK+PL  IQ  N
Sbjct: 351 RAKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ--N 410

Query: 440 DPIAPDRAIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGAPWTDPFVIDFLVHLE 495
           D + P   IPR  I ENP   L++     +L      G  +   W      ++L  +E
Sbjct: 411 DDVVPPYTIPRSSIAENPFTSLLLCSSSPNL----IDGRTVAVSWCQDLASEWLTAVE 458