Lag0032565 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032565
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon RE1
Locationchr11: 34725192 .. 34726223 (+)
RNA-Seq ExpressionLag0032565
SyntenyLag0032565
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGTTTCATTGATGGTACTCCGGCTCCTCCGAAGTTTTTGGATCCCGCCCACACTCAACTAAATCCTCTGTTTACTGTATGGCAAAAGTATAATCGGACACTGATGAGTTGGATTTATTCCTCTTTGAATGAAGACGAATTAGGTGAAATAATAGGATGTTCTTCTGCTTATGAAATCTGGGATCATTTGCGTATTGTTTATGAATCGTCTTCTACTGCACGAATTATGAGCTTAAGGTCTCAGCTTCAGAAAATCAAGAAGGATGGTCTGTCGGTGTCTCAGTACCTTGCCCAAATCAAAGATATAGCCGACAAATTCTCCGCCATTGGTGAACCACTGTCGTATCTTGACCATCTCGGCTATATCCTTGAGGGGCTCGAGTCTGAGTACAATGCTTTCGTCACCTCTGTTCACAACAGAACAAACCGCCCTTCCCTTGCCGACCTTCGGAGTCTTCTCATTGCTTATGATGCTCGTCTGGAAAAACAAACTTCGGTGGACCAACTGAACCTGATTCAGGCCAATCTTGCAAACTTGTCTTCCAATCCTTCGTCACAACCTAAGCGGTTTCAGCGTTCTTCAAATCCTCCTAAACCTAATTCAAATTCGAGGCAACCTTCCCCTTTTCCCTTTCCACCCAATTTCTCTTTTTCCCCTAGTCCTGGTGTGTTGGGTCGGCCTCAGACTTCTCCTCGTCCTCCCAGATGGCCTTCTAATAATCAAAACCGTCCTCAGTGTCATATTTGCGGCAAGTTTGGACACACAACTCTTGTGTGTTATAATCGTCACAATCCTGTTTATCAAGCTCCACAAGTTTCTTCCCCTCCACAAGCCTTTTTTAACCAATTTTCAGCTTCCTCTGCCCCTGTTAGTCAATCAGTCCCTGTTTCCGCCTCTTCATCCACCTTTATCCCTCTCACATCCCACCCTGATGAATCCTGGTTCATGGACTCGGGTGCCACTCATCACATGACCCCCGACTTCAATACCATTCAGCATCCATCCCCATACTATGGCAGTGAGTAG

mRNA sequence

ATGGAGAGTTTCATTGATGGTACTCCGGCTCCTCCGAAGTTTTTGGATCCCGCCCACACTCAACTAAATCCTCTGTTTACTGTATGGCAAAAGTATAATCGGACACTGATGAGTTGGATTTATTCCTCTTTGAATGAAGACGAATTAGGTGAAATAATAGGATGTTCTTCTGCTTATGAAATCTGGGATCATTTGCGTATTGTTTATGAATCGTCTTCTACTGCACGAATTATGAGCTTAAGGTCTCAGCTTCAGAAAATCAAGAAGGATGGTCTGTCGGTGTCTCAGTACCTTGCCCAAATCAAAGATATAGCCGACAAATTCTCCGCCATTGGTGAACCACTGTCGTATCTTGACCATCTCGGCTATATCCTTGAGGGGCTCGAGTCTGAGTACAATGCTTTCGTCACCTCTGTTCACAACAGAACAAACCGCCCTTCCCTTGCCGACCTTCGGAGTCTTCTCATTGCTTATGATGCTCGTCTGGAAAAACAAACTTCGGTGGACCAACTGAACCTGATTCAGGCCAATCTTGCAAACTTGTCTTCCAATCCTTCGTCACAACCTAAGCGGTTTCAGCGTTCTTCAAATCCTCCTAAACCTAATTCAAATTCGAGGCAACCTTCCCCTTTTCCCTTTCCACCCAATTTCTCTTTTTCCCCTAGTCCTGGTGTGTTGGGTCGGCCTCAGACTTCTCCTCGTCCTCCCAGATGGCCTTCTAATAATCAAAACCGTCCTCAGTGTCATATTTGCGGCAAGTTTGGACACACAACTCTTGTGTGTTATAATCGTCACAATCCTGTTTATCAAGCTCCACAAGTTTCTTCCCCTCCACAAGCCTTTTTTAACCAATTTTCAGCTTCCTCTGCCCCTGTTAGTCAATCAGTCCCTGTTTCCGCCTCTTCATCCACCTTTATCCCTCTCACATCCCACCCTGATGAATCCTGGTTCATGGACTCGGGTGCCACTCATCACATGACCCCCGACTTCAATACCATTCAGCATCCATCCCCATACTATGGCAGTGAGTAG

Coding sequence (CDS)

ATGGAGAGTTTCATTGATGGTACTCCGGCTCCTCCGAAGTTTTTGGATCCCGCCCACACTCAACTAAATCCTCTGTTTACTGTATGGCAAAAGTATAATCGGACACTGATGAGTTGGATTTATTCCTCTTTGAATGAAGACGAATTAGGTGAAATAATAGGATGTTCTTCTGCTTATGAAATCTGGGATCATTTGCGTATTGTTTATGAATCGTCTTCTACTGCACGAATTATGAGCTTAAGGTCTCAGCTTCAGAAAATCAAGAAGGATGGTCTGTCGGTGTCTCAGTACCTTGCCCAAATCAAAGATATAGCCGACAAATTCTCCGCCATTGGTGAACCACTGTCGTATCTTGACCATCTCGGCTATATCCTTGAGGGGCTCGAGTCTGAGTACAATGCTTTCGTCACCTCTGTTCACAACAGAACAAACCGCCCTTCCCTTGCCGACCTTCGGAGTCTTCTCATTGCTTATGATGCTCGTCTGGAAAAACAAACTTCGGTGGACCAACTGAACCTGATTCAGGCCAATCTTGCAAACTTGTCTTCCAATCCTTCGTCACAACCTAAGCGGTTTCAGCGTTCTTCAAATCCTCCTAAACCTAATTCAAATTCGAGGCAACCTTCCCCTTTTCCCTTTCCACCCAATTTCTCTTTTTCCCCTAGTCCTGGTGTGTTGGGTCGGCCTCAGACTTCTCCTCGTCCTCCCAGATGGCCTTCTAATAATCAAAACCGTCCTCAGTGTCATATTTGCGGCAAGTTTGGACACACAACTCTTGTGTGTTATAATCGTCACAATCCTGTTTATCAAGCTCCACAAGTTTCTTCCCCTCCACAAGCCTTTTTTAACCAATTTTCAGCTTCCTCTGCCCCTGTTAGTCAATCAGTCCCTGTTTCCGCCTCTTCATCCACCTTTATCCCTCTCACATCCCACCCTGATGAATCCTGGTTCATGGACTCGGGTGCCACTCATCACATGACCCCCGACTTCAATACCATTCAGCATCCATCCCCATACTATGGCAGTGAGTAG

Protein sequence

MESFIDGTPAPPKFLDPAHTQLNPLFTVWQKYNRTLMSWIYSSLNEDELGEIIGCSSAYEIWDHLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQYLAQIKDIADKFSAIGEPLSYLDHLGYILEGLESEYNAFVTSVHNRTNRPSLADLRSLLIAYDARLEKQTSVDQLNLIQANLANLSSNPSSQPKRFQRSSNPPKPNSNSRQPSPFPFPPNFSFSPSPGVLGRPQTSPRPPRWPSNNQNRPQCHICGKFGHTTLVCYNRHNPVYQAPQVSSPPQAFFNQFSASSAPVSQSVPVSASSSTFIPLTSHPDESWFMDSGATHHMTPDFNTIQHPSPYYGSE
Homology
BLAST of Lag0032565 vs. NCBI nr
Match: XP_022155181.1 (uncharacterized protein LOC111022315 [Momordica charantia])

HSP 1 Score: 309.7 bits (792), Expect = 3.2e-80
Identity = 170/344 (49.42%), Postives = 230/344 (66.86%), Query Frame = 0

Query: 1   MESFIDGT-PAPPKFLDPAHTQLNPLFTVWQKYNRTLMSWIYSSLNEDELGEIIGCSSAY 60
           +  ++DGT   PP+FLD    Q NP +  W++YNR LM WIYSSL+E+++GE++   + +
Sbjct: 55  LRGYLDGTIMPPPQFLDHHQLQPNPAYYAWERYNRLLMCWIYSSLSEEKMGEVVSLETTH 114

Query: 61  EIWDHLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQYLAQIKDIADKFSAIGEPLSYLD 120
           +IW  L  VY+S +TARIM L+++LQ ++KDG SVSQYLA+IK+IADKF+A+GEPLSY D
Sbjct: 115 DIWSSLTRVYDSKTTARIMGLKTELQNLRKDGSSVSQYLAKIKEIADKFAAVGEPLSYRD 174

Query: 121 HLGYILEGLESEYNAFVTSVHNRTNRPSLADLRSLLIAYDARLEKQTSVDQLNLIQANLA 180
           HL ++L+GL SEYNAFVTS+HNR + PSL D+RSLL+AY+ARL+KQ +VDQLN+ QANL 
Sbjct: 175 HLAHVLDGLGSEYNAFVTSIHNRADSPSLEDVRSLLLAYEARLDKQNTVDQLNIAQANLV 234

Query: 181 NLSSNPSSQPKRFQRSSNPPKPNSNSRQPSPFPFPPNFSFSPSPGVLGRPQTSPRPPRWP 240
           NLS   +S+         PPK +  +     FP  P  S + S  +LG+PQ+  + P  P
Sbjct: 235 NLSLQHNSK-------RPPPKFSFPNHYKHSFPNSP-ISAAQSQSILGKPQSVHKWPPKP 294

Query: 241 SNNQNRPQCHICGKFGHTTLVCYNRHNPVYQAPQVSSPPQAFFNQFSASSAPVSQSVPVS 300
           S+  ++ QC ICGK GH+  VCY+R N  Y     ++ PQA ++    S        P  
Sbjct: 295 SS--SKIQCQICGKLGHSAAVCYHRTNIAYH----NASPQALYHHVQPS--------PTH 354

Query: 301 ASSSTFIPLTSHPDESWFMDSGATHHMTPDFNTIQHPSPYYGSE 344
            SS        HPDESWFMDSGATHHMTPD + + +P+PY G E
Sbjct: 355 PSSGHEF---QHPDESWFMDSGATHHMTPDSSILCNPTPYSGGE 373

BLAST of Lag0032565 vs. NCBI nr
Match: RVW69807.1 (Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera])

HSP 1 Score: 213.4 bits (542), Expect = 3.1e-51
Identity = 131/340 (38.53%), Postives = 177/340 (52.06%), Query Frame = 0

Query: 1   MESFID-GTPAPPKFLDPAHTQLNPLFTVWQKYNRTLMSWIYSSLNEDELGEIIGCSSAY 60
           +E FID    +PPK+LD A  Q+NP F  W + N+ +MSWIYSSL    +G+I+  S+A 
Sbjct: 65  LEDFIDPDQSSPPKYLDAACRQVNPEFVQWDRLNQLVMSWIYSSLTPGMVGQIVEYSTAR 124

Query: 61  EIWDHLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQYLAQIKDIADKFSAIGEPLSYLD 120
           +IW  L   YES S A +MSL SQLQ+IKK  + +S+YL+++K + D+F+ IGEPLSY D
Sbjct: 125 DIWASLNDEYESPSIATVMSLNSQLQRIKKIDIPLSEYLSRLKFVFDEFATIGEPLSYRD 184

Query: 121 HLGYILEGLESEYNAFVTSVHNRTNRPSLADLRSLLIAYDARLEKQTSVDQLNLIQANLA 180
            L  ILEGL  EY+ FVTS+HNR++RPSL ++ SLL  Y+ RL +++    LN  QAN  
Sbjct: 185 KLTRILEGLPEEYDNFVTSIHNRSDRPSLQEVHSLLHTYEYRLSQRSMDQNLNFPQAN-- 244

Query: 181 NLSSNPSSQPKRFQRSSNPPKPNSNSRQPSPFPFPPNFSFSPSPGVLGRPQTSPRPPRWP 240
                                                                   PR P
Sbjct: 245 --------------------------------------------------------PRQP 304

Query: 241 SNNQNRPQCHICGKFGHTTLVCYNRHNPVYQAPQVSSPPQAFFNQFSASSAPVSQSVPVS 300
             N + PQC ICGK GH  L  Y+R N  Y  P   +      N    +S+P+S  +  S
Sbjct: 305 GYNNSIPQCQICGKSGHIALNGYHRTNLTYHPPVFPNAAAFNPNGPGQTSSPISAMLTTS 344

Query: 301 ASSSTFIPLTSHPDESWFMDSGATHHMTPDFNTIQHPSPY 340
           A+ +     +S  D SW+MDSGATHH TP+F  +     Y
Sbjct: 365 AAPTGLSSQSS--DNSWYMDSGATHHFTPEFGHMTDAMQY 344

BLAST of Lag0032565 vs. NCBI nr
Match: GFY82848.1 (hypothetical protein Acr_02g0010880 [Actinidia rufa])

HSP 1 Score: 213.4 bits (542), Expect = 3.1e-51
Identity = 132/307 (43.00%), Postives = 185/307 (60.26%), Query Frame = 0

Query: 37  MSWIYSSLNEDELGEIIGCSSAYEIWDHLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQ 96
           MSWIY+S+NE  LG+I+G +SA +IW+ L  +Y ++S A +  LR+ LQ IKKDGL+   
Sbjct: 1   MSWIYASINEFMLGQIVGYTSASQIWEALERLYAAASFAHLTELRTALQTIKKDGLTALA 60

Query: 97  YLAQIKDIADKFSAIGEPLSYLDHLGYILEGLESEYNAFVTSVHNRTNRPSLADLRSLLI 156
           Y+ + + + +  ++IGEP++Y DHL Y L GL  +YN FVTS+ ++  RPS+ ++ SLL+
Sbjct: 61  YIQKFRHLCNSLASIGEPVTYTDHLIYFLGGLGRDYNPFVTSIQSQAIRPSIEEVHSLLL 120

Query: 157 AYDARLEKQTSVDQLNLIQANLANLSSNPSSQPKRFQRSSNPPKPNSNSRQPSPFPFPPN 216
           +YDARLE+Q++ D L+ +QANLANL    + Q  +F+  S    PNSNS    P     N
Sbjct: 121 SYDARLERQSATDTLSSLQANLANL----TYQKPKFKNPSTNSFPNSNS-YSHPRGQNRN 180

Query: 217 FSFSPSPGVLGRPQTSPRPPRWPSNNQNRPQCHICGKFGHTTLVCYNRHNPVYQAPQVSS 276
            S+SP+P       +SP+P         RP+C IC K GHT   CY+R N  YQ P    
Sbjct: 181 PSYSPNP-------SSPKP---------RPRCQICLKPGHTANKCYHRTNLNYQPP---P 240

Query: 277 PPQAFFNQFSASSAPVSQSVPVSASSSTFIPLTSHPDESWFMDSGATHHMTPDFNTIQHP 336
           PP   FN +   +  +S S    AS+S      S PD SW+MDSGA+HH TP  N +   
Sbjct: 241 PPPRNFNAYITPNPSISTSSNQPASAS------SLPDSSWYMDSGASHHFTPYLNMLDSA 277

Query: 337 SPYYGSE 344
           SPY G +
Sbjct: 301 SPYTGMD 277

BLAST of Lag0032565 vs. NCBI nr
Match: XP_022148871.1 (uncharacterized protein LOC111017438 [Momordica charantia])

HSP 1 Score: 209.9 bits (533), Expect = 3.4e-50
Identity = 127/273 (46.52%), Postives = 171/273 (62.64%), Query Frame = 0

Query: 83  QLQKIKKDGLSVSQYLAQIKDIADKFSAIGEPLSYLDHLGYILEGLESEYNAFVTSVHNR 142
           ++Q++KKDGLSVSQYLA+IK+I  K S+IGEP+S  DH+ YI+EGL  EYNAFVTS+ NR
Sbjct: 7   EIQQVKKDGLSVSQYLAKIKEITGKLSSIGEPISLKDHISYIIEGLGIEYNAFVTSIQNR 66

Query: 143 TNRPSLADLRSLLIAYDARLEKQTSVDQLNLIQANLANLSSNPSSQPKRFQRSSNPPKPN 202
           ++  +L D+R+LL+AYD RLEKQ SVDQLN++QAN+ANL  N  S   R           
Sbjct: 67  SDMXTLEDVRTLLLAYDYRLEKQNSVDQLNVVQANVANLQLNRQSGHTR----------- 126

Query: 203 SNSRQPSPFPFPPNFSFSPSPGVLGRPQTSPRPPRWPSNNQN----RPQCHICGKFGHTT 262
            N R PS    P  F+F   PG+LG+P  + +PP WP + Q+    + QC IC K GHTT
Sbjct: 127 -NHRVPSQHS-PRPFNFKTQPGLLGKPNQNSQPP-WPPSQQSPRNPKVQCQICFKLGHTT 186

Query: 263 LVCYNRHNPVYQA---PQVSSPPQAFFNQFSASSAPVSQS-----VPVSASSSTFIPLTS 322
             CY+R N  Y+    P     P A F+  ++SS+   QS     VP + +  +F  L  
Sbjct: 187 SKCYHRANLQYKLNFFPTNQPNPHALFHHVNSSSSQFQQSNPTANVPPTPNPFSFPNLIV 246

Query: 323 HPDESWFMDSGATHHMTPDFNTIQHPSPYYGSE 344
            PD+ W+MDSGATHH++ D N++ +   Y G E
Sbjct: 247 IPDDLWYMDSGATHHISHDINSLFNLMXYTGGE 265

BLAST of Lag0032565 vs. NCBI nr
Match: GFZ12741.1 (UBX domain-containing protein [Actinidia rufa])

HSP 1 Score: 207.6 bits (527), Expect = 1.7e-49
Identity = 133/344 (38.66%), Postives = 187/344 (54.36%), Query Frame = 0

Query: 1   MESFIDGT-PAPPKFLDPAHTQLNPLFTVWQKYNRTLMSWIYSSLNEDELGEIIGCSSAY 60
           +E F+DG+   PP+FLDP   Q NP F  WQ+YNR +MSWIY+S+NE  LG+I+G +SA 
Sbjct: 76  LEEFLDGSRVCPPRFLDPQQQQSNPEFHSWQRYNRLVMSWIYASINESMLGQIVGYTSAS 135

Query: 61  EIWDHLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQYLAQIKDIADKFSAIGEPLSYLD 120
           +IW+ L  +Y ++S A +  LR+ LQ IKK+GL+   Y+ + + + +  ++IGEP++Y D
Sbjct: 136 QIWEALERLYAAASFAHLTELRTALQTIKKEGLTALAYIQKFRHLCNSLASIGEPVTYTD 195

Query: 121 HLGYILEGLESEYNAFVTSVHNRTNRPSLADLRSLLIAYDARLEKQTSVDQLNLIQANLA 180
           HL Y L GL  +YN FVTS+ ++  RPS              +E+ TS   L        
Sbjct: 196 HLIYFLGGLGRDYNPFVTSIQSQAIRPS--------------VEEPTSPTSL-------- 255

Query: 181 NLSSNPSSQPKRFQRSSNPPKPNSNSRQPSPFPFPPNFSFSPSPGVLGRPQTSPRPPRWP 240
                  ++  +F+  S    PNSNS    P     N S+SP+P       +SP+P    
Sbjct: 256 -------TRKPKFKNPSTNSFPNSNS-YSHPRGQNRNPSYSPNP-------SSPKP---- 315

Query: 241 SNNQNRPQCHICGKFGHTTLVCYNRHNPVYQAPQVSSPPQAFFNQFSASSAPVSQSVPVS 300
                RP+C IC K GHT   CY+  N  YQ P    PP   FN +   +  +S S    
Sbjct: 316 -----RPRCQICLKPGHTANKCYHHTNLNYQPP---PPPPRNFNAYITPNPSLSTS---- 365

Query: 301 ASSSTFIPLTSHPDESWFMDSGATHHMTPDFNTIQHPSPYYGSE 344
            S+   + L S PD SW+MDSGA+HH TPD N +   SPY G +
Sbjct: 376 -SNQPAMLLLSLPDSSWYMDSGASHHFTPDLNMLDSASPYTGMD 365

BLAST of Lag0032565 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 7.2e-19
Identity = 76/348 (21.84%), Postives = 158/348 (45.40%), Query Frame = 0

Query: 1   MESFIDG-TPAPPKFL-DPAHTQLNPLFTVWQKYNRTLMSWIYSSLNEDELGEIIGCSSA 60
           +  F+DG T  PP  +   A  ++NP +T W++ ++ + S +  +++      +   ++A
Sbjct: 47  LAGFLDGSTTMPPATIGTDAAPRVNPDYTRWKRQDKLIYSAVLGAISMSVQPAVSRATTA 106

Query: 61  YEIWDHLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQYLAQIKDIADKFSAIGEPLSYL 120
            +IW+ LR +Y + S   +  LR+QL++  K   ++  Y+  +    D+ + +G+P+ + 
Sbjct: 107 AQIWETLRKIYANPSYGHVTQLRTQLKQWTKGTKTIDDYMQGLVTRFDQLALLGKPMDHD 166

Query: 121 DHLGYILEGLESEYNAFVTSVHNRTNRPSLADLRSLLIAYDARLEKQTSVDQLNLIQANL 180
           + +  +LE L  EY   +  +  +   P+L ++   L+ +++++   +S   + +    +
Sbjct: 167 EQVERVLENLPEEYKPVIDQIAAKDTPPTLTEIHERLLNHESKILAVSSATVIPITANAV 226

Query: 181 ANLSSNPSSQPKRFQRSSNPPKPNSNSRQPSPFPFPPNFSFSPSPGVLGRPQTSPRPPRW 240
           ++ ++  ++      R++     N+N+          NF                     
Sbjct: 227 SHRNTTTTNNNNNGNRNNRYDNRNNNNNSKPWQQSSTNFH-------------------- 286

Query: 241 PSNNQNRP---QCHICGKFGHTTLVCYNRHNPVYQAPQVSSPPQAFFNQFSASSAPVSQS 300
           P+NNQ++P   +C ICG  GH+   C              S  Q F +  + S  P S  
Sbjct: 287 PNNNQSKPYLGKCQICGVQGHSAKRC--------------SQLQHFLSSVN-SQQPPSPF 346

Query: 301 VPVSASSSTFIPLTSHPDESWFMDSGATHHMTPDFNTIQHPSPYYGSE 344
            P    ++  +  + +   +W +DSGATHH+T DFN +    PY G +
Sbjct: 347 TPWQPRANLALG-SPYSSNNWLLDSGATHHITSDFNNLSLHQPYTGGD 358

BLAST of Lag0032565 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.2e-13
Identity = 84/348 (24.14%), Postives = 153/348 (43.97%), Query Frame = 0

Query: 1   MESFIDG-TPAPPKFL-DPAHTQLNPLFTVWQKYNRTLMSWIYSSLNEDELGEIIGCSSA 60
           +  F+DG TP PP  +   A  ++NP +T W++ ++ + S I  +++      +   ++A
Sbjct: 47  LAGFLDGSTPMPPATIGTDAVPRVNPDYTRWRRQDKLIYSAILGAISMSVQPAVSRATTA 106

Query: 61  YEIWDHLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQYLAQIKDIADKFSAIGEPLSYL 120
            +IW+ LR +Y + S   +  LR               ++ +     D+ + +G+P+ + 
Sbjct: 107 AQIWETLRKIYANPSYGHVTQLR---------------FITRF----DQLALLGKPMDHD 166

Query: 121 DHLGYILEGLESEYNAFVTSVHNRTNRPSLADLRSLLIAYDARLEKQTSVDQLNLIQANL 180
           + +  +LE L  +Y   +  +  +   PSL ++   LI  +++L    S + +  I AN+
Sbjct: 167 EQVERVLENLPDDYKPVIDQIAAKDTPPSLTEIHERLINRESKLLALNSAEVVP-ITANV 226

Query: 181 ANLSSNPSSQPKRFQRSSNPPKPNSNSRQPSPFPFPPNFSFSPSPGVLGRPQTSPRPPRW 240
                N ++   +  R  N    N+N+R  S   + P+ S S S                
Sbjct: 227 VT-HRNTNTNRNQNNRGDNRNYNNNNNRSNS---WQPSSSGSRS---------------- 286

Query: 241 PSNNQNRP---QCHICGKFGHTTLVCYNRHNPVYQAPQVSSPPQAFFNQFSASSAPVSQS 300
             N Q +P   +C IC   GH+   C   H             Q+  NQ   S++P +  
Sbjct: 287 -DNRQPKPYLGRCQICSVQGHSAKRCPQLHQ-----------FQSTTNQ-QQSTSPFTPW 337

Query: 301 VPVSASSSTFIPLTSHPDESWFMDSGATHHMTPDFNTIQHPSPYYGSE 344
            P  A+ +   P  ++   +W +DSGATHH+T DFN +    PY G +
Sbjct: 347 QP-RANLAVNSPYNAN---NWLLDSGATHHITSDFNNLSFHQPYTGGD 337

BLAST of Lag0032565 vs. ExPASy TrEMBL
Match: A0A6J1DQX7 (uncharacterized protein LOC111022315 OS=Momordica charantia OX=3673 GN=LOC111022315 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.5e-80
Identity = 170/344 (49.42%), Postives = 230/344 (66.86%), Query Frame = 0

Query: 1   MESFIDGT-PAPPKFLDPAHTQLNPLFTVWQKYNRTLMSWIYSSLNEDELGEIIGCSSAY 60
           +  ++DGT   PP+FLD    Q NP +  W++YNR LM WIYSSL+E+++GE++   + +
Sbjct: 55  LRGYLDGTIMPPPQFLDHHQLQPNPAYYAWERYNRLLMCWIYSSLSEEKMGEVVSLETTH 114

Query: 61  EIWDHLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQYLAQIKDIADKFSAIGEPLSYLD 120
           +IW  L  VY+S +TARIM L+++LQ ++KDG SVSQYLA+IK+IADKF+A+GEPLSY D
Sbjct: 115 DIWSSLTRVYDSKTTARIMGLKTELQNLRKDGSSVSQYLAKIKEIADKFAAVGEPLSYRD 174

Query: 121 HLGYILEGLESEYNAFVTSVHNRTNRPSLADLRSLLIAYDARLEKQTSVDQLNLIQANLA 180
           HL ++L+GL SEYNAFVTS+HNR + PSL D+RSLL+AY+ARL+KQ +VDQLN+ QANL 
Sbjct: 175 HLAHVLDGLGSEYNAFVTSIHNRADSPSLEDVRSLLLAYEARLDKQNTVDQLNIAQANLV 234

Query: 181 NLSSNPSSQPKRFQRSSNPPKPNSNSRQPSPFPFPPNFSFSPSPGVLGRPQTSPRPPRWP 240
           NLS   +S+         PPK +  +     FP  P  S + S  +LG+PQ+  + P  P
Sbjct: 235 NLSLQHNSK-------RPPPKFSFPNHYKHSFPNSP-ISAAQSQSILGKPQSVHKWPPKP 294

Query: 241 SNNQNRPQCHICGKFGHTTLVCYNRHNPVYQAPQVSSPPQAFFNQFSASSAPVSQSVPVS 300
           S+  ++ QC ICGK GH+  VCY+R N  Y     ++ PQA ++    S        P  
Sbjct: 295 SS--SKIQCQICGKLGHSAAVCYHRTNIAYH----NASPQALYHHVQPS--------PTH 354

Query: 301 ASSSTFIPLTSHPDESWFMDSGATHHMTPDFNTIQHPSPYYGSE 344
            SS        HPDESWFMDSGATHHMTPD + + +P+PY G E
Sbjct: 355 PSSGHEF---QHPDESWFMDSGATHHMTPDSSILCNPTPYSGGE 373

BLAST of Lag0032565 vs. ExPASy TrEMBL
Match: A0A438GC62 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=29760 GN=RE1_210 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.5e-51
Identity = 131/340 (38.53%), Postives = 177/340 (52.06%), Query Frame = 0

Query: 1   MESFID-GTPAPPKFLDPAHTQLNPLFTVWQKYNRTLMSWIYSSLNEDELGEIIGCSSAY 60
           +E FID    +PPK+LD A  Q+NP F  W + N+ +MSWIYSSL    +G+I+  S+A 
Sbjct: 65  LEDFIDPDQSSPPKYLDAACRQVNPEFVQWDRLNQLVMSWIYSSLTPGMVGQIVEYSTAR 124

Query: 61  EIWDHLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQYLAQIKDIADKFSAIGEPLSYLD 120
           +IW  L   YES S A +MSL SQLQ+IKK  + +S+YL+++K + D+F+ IGEPLSY D
Sbjct: 125 DIWASLNDEYESPSIATVMSLNSQLQRIKKIDIPLSEYLSRLKFVFDEFATIGEPLSYRD 184

Query: 121 HLGYILEGLESEYNAFVTSVHNRTNRPSLADLRSLLIAYDARLEKQTSVDQLNLIQANLA 180
            L  ILEGL  EY+ FVTS+HNR++RPSL ++ SLL  Y+ RL +++    LN  QAN  
Sbjct: 185 KLTRILEGLPEEYDNFVTSIHNRSDRPSLQEVHSLLHTYEYRLSQRSMDQNLNFPQAN-- 244

Query: 181 NLSSNPSSQPKRFQRSSNPPKPNSNSRQPSPFPFPPNFSFSPSPGVLGRPQTSPRPPRWP 240
                                                                   PR P
Sbjct: 245 --------------------------------------------------------PRQP 304

Query: 241 SNNQNRPQCHICGKFGHTTLVCYNRHNPVYQAPQVSSPPQAFFNQFSASSAPVSQSVPVS 300
             N + PQC ICGK GH  L  Y+R N  Y  P   +      N    +S+P+S  +  S
Sbjct: 305 GYNNSIPQCQICGKSGHIALNGYHRTNLTYHPPVFPNAAAFNPNGPGQTSSPISAMLTTS 344

Query: 301 ASSSTFIPLTSHPDESWFMDSGATHHMTPDFNTIQHPSPY 340
           A+ +     +S  D SW+MDSGATHH TP+F  +     Y
Sbjct: 365 AAPTGLSSQSS--DNSWYMDSGATHHFTPEFGHMTDAMQY 344

BLAST of Lag0032565 vs. ExPASy TrEMBL
Match: A0A7J0E8R3 (Uncharacterized protein OS=Actinidia rufa OX=165716 GN=Acr_02g0010880 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.5e-51
Identity = 132/307 (43.00%), Postives = 185/307 (60.26%), Query Frame = 0

Query: 37  MSWIYSSLNEDELGEIIGCSSAYEIWDHLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQ 96
           MSWIY+S+NE  LG+I+G +SA +IW+ L  +Y ++S A +  LR+ LQ IKKDGL+   
Sbjct: 1   MSWIYASINEFMLGQIVGYTSASQIWEALERLYAAASFAHLTELRTALQTIKKDGLTALA 60

Query: 97  YLAQIKDIADKFSAIGEPLSYLDHLGYILEGLESEYNAFVTSVHNRTNRPSLADLRSLLI 156
           Y+ + + + +  ++IGEP++Y DHL Y L GL  +YN FVTS+ ++  RPS+ ++ SLL+
Sbjct: 61  YIQKFRHLCNSLASIGEPVTYTDHLIYFLGGLGRDYNPFVTSIQSQAIRPSIEEVHSLLL 120

Query: 157 AYDARLEKQTSVDQLNLIQANLANLSSNPSSQPKRFQRSSNPPKPNSNSRQPSPFPFPPN 216
           +YDARLE+Q++ D L+ +QANLANL    + Q  +F+  S    PNSNS    P     N
Sbjct: 121 SYDARLERQSATDTLSSLQANLANL----TYQKPKFKNPSTNSFPNSNS-YSHPRGQNRN 180

Query: 217 FSFSPSPGVLGRPQTSPRPPRWPSNNQNRPQCHICGKFGHTTLVCYNRHNPVYQAPQVSS 276
            S+SP+P       +SP+P         RP+C IC K GHT   CY+R N  YQ P    
Sbjct: 181 PSYSPNP-------SSPKP---------RPRCQICLKPGHTANKCYHRTNLNYQPP---P 240

Query: 277 PPQAFFNQFSASSAPVSQSVPVSASSSTFIPLTSHPDESWFMDSGATHHMTPDFNTIQHP 336
           PP   FN +   +  +S S    AS+S      S PD SW+MDSGA+HH TP  N +   
Sbjct: 241 PPPRNFNAYITPNPSISTSSNQPASAS------SLPDSSWYMDSGASHHFTPYLNMLDSA 277

Query: 337 SPYYGSE 344
           SPY G +
Sbjct: 301 SPYTGMD 277

BLAST of Lag0032565 vs. ExPASy TrEMBL
Match: A0A6J1D6N7 (uncharacterized protein LOC111017438 OS=Momordica charantia OX=3673 GN=LOC111017438 PE=4 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 1.7e-50
Identity = 127/273 (46.52%), Postives = 171/273 (62.64%), Query Frame = 0

Query: 83  QLQKIKKDGLSVSQYLAQIKDIADKFSAIGEPLSYLDHLGYILEGLESEYNAFVTSVHNR 142
           ++Q++KKDGLSVSQYLA+IK+I  K S+IGEP+S  DH+ YI+EGL  EYNAFVTS+ NR
Sbjct: 7   EIQQVKKDGLSVSQYLAKIKEITGKLSSIGEPISLKDHISYIIEGLGIEYNAFVTSIQNR 66

Query: 143 TNRPSLADLRSLLIAYDARLEKQTSVDQLNLIQANLANLSSNPSSQPKRFQRSSNPPKPN 202
           ++  +L D+R+LL+AYD RLEKQ SVDQLN++QAN+ANL  N  S   R           
Sbjct: 67  SDMXTLEDVRTLLLAYDYRLEKQNSVDQLNVVQANVANLQLNRQSGHTR----------- 126

Query: 203 SNSRQPSPFPFPPNFSFSPSPGVLGRPQTSPRPPRWPSNNQN----RPQCHICGKFGHTT 262
            N R PS    P  F+F   PG+LG+P  + +PP WP + Q+    + QC IC K GHTT
Sbjct: 127 -NHRVPSQHS-PRPFNFKTQPGLLGKPNQNSQPP-WPPSQQSPRNPKVQCQICFKLGHTT 186

Query: 263 LVCYNRHNPVYQA---PQVSSPPQAFFNQFSASSAPVSQS-----VPVSASSSTFIPLTS 322
             CY+R N  Y+    P     P A F+  ++SS+   QS     VP + +  +F  L  
Sbjct: 187 SKCYHRANLQYKLNFFPTNQPNPHALFHHVNSSSSQFQQSNPTANVPPTPNPFSFPNLIV 246

Query: 323 HPDESWFMDSGATHHMTPDFNTIQHPSPYYGSE 344
            PD+ W+MDSGATHH++ D N++ +   Y G E
Sbjct: 247 IPDDLWYMDSGATHHISHDINSLFNLMXYTGGE 265

BLAST of Lag0032565 vs. ExPASy TrEMBL
Match: A0A7J0GPN0 (UBX domain-containing protein OS=Actinidia rufa OX=165716 GN=Acr_23g0011260 PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 8.2e-50
Identity = 133/344 (38.66%), Postives = 187/344 (54.36%), Query Frame = 0

Query: 1   MESFIDGT-PAPPKFLDPAHTQLNPLFTVWQKYNRTLMSWIYSSLNEDELGEIIGCSSAY 60
           +E F+DG+   PP+FLDP   Q NP F  WQ+YNR +MSWIY+S+NE  LG+I+G +SA 
Sbjct: 76  LEEFLDGSRVCPPRFLDPQQQQSNPEFHSWQRYNRLVMSWIYASINESMLGQIVGYTSAS 135

Query: 61  EIWDHLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQYLAQIKDIADKFSAIGEPLSYLD 120
           +IW+ L  +Y ++S A +  LR+ LQ IKK+GL+   Y+ + + + +  ++IGEP++Y D
Sbjct: 136 QIWEALERLYAAASFAHLTELRTALQTIKKEGLTALAYIQKFRHLCNSLASIGEPVTYTD 195

Query: 121 HLGYILEGLESEYNAFVTSVHNRTNRPSLADLRSLLIAYDARLEKQTSVDQLNLIQANLA 180
           HL Y L GL  +YN FVTS+ ++  RPS              +E+ TS   L        
Sbjct: 196 HLIYFLGGLGRDYNPFVTSIQSQAIRPS--------------VEEPTSPTSL-------- 255

Query: 181 NLSSNPSSQPKRFQRSSNPPKPNSNSRQPSPFPFPPNFSFSPSPGVLGRPQTSPRPPRWP 240
                  ++  +F+  S    PNSNS    P     N S+SP+P       +SP+P    
Sbjct: 256 -------TRKPKFKNPSTNSFPNSNS-YSHPRGQNRNPSYSPNP-------SSPKP---- 315

Query: 241 SNNQNRPQCHICGKFGHTTLVCYNRHNPVYQAPQVSSPPQAFFNQFSASSAPVSQSVPVS 300
                RP+C IC K GHT   CY+  N  YQ P    PP   FN +   +  +S S    
Sbjct: 316 -----RPRCQICLKPGHTANKCYHHTNLNYQPP---PPPPRNFNAYITPNPSLSTS---- 365

Query: 301 ASSSTFIPLTSHPDESWFMDSGATHHMTPDFNTIQHPSPYYGSE 344
            S+   + L S PD SW+MDSGA+HH TPD N +   SPY G +
Sbjct: 376 -SNQPAMLLLSLPDSSWYMDSGASHHFTPDLNMLDSASPYTGMD 365

BLAST of Lag0032565 vs. TAIR 10
Match: AT1G21280.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); Has 707 Blast hits to 705 proteins in 25 species: Archae - 0; Bacteria - 0; Metazoa - 4; Fungi - 0; Plants - 703; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 56.2 bits (134), Expect = 5.9e-08
Identity = 29/101 (28.71%), Postives = 55/101 (54.46%), Query Frame = 0

Query: 4   FIDGTPAPPKFLDPAHTQLNPLFTVWQKYNRTLMSWIYSSLNEDELGEIIGCSSAYEIWD 63
           FIDGT   P   DP     +PL+  W++ N  +M W+ +S+ +  L  ++   +A+++W+
Sbjct: 61  FIDGTLPKP---DP----FSPLYQPWEQCNAMVMYWLMNSMTDKLLESVMYAETAHKMWE 120

Query: 64  HLRIVYESSSTARIMSLRSQLQKIKKDGLSVSQYLAQIKDI 105
            LR V+      +I  LR +L  +++ G SV +Y  ++  +
Sbjct: 121 DLRRVFVPCVDLKIYQLRRRLATLRQGGDSVEEYFGKLSKV 154

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155181.13.2e-8049.42uncharacterized protein LOC111022315 [Momordica charantia][more]
RVW69807.13.1e-5138.53Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera][more]
GFY82848.13.1e-5143.00hypothetical protein Acr_02g0010880 [Actinidia rufa][more]
XP_022148871.13.4e-5046.52uncharacterized protein LOC111017438 [Momordica charantia][more]
GFZ12741.11.7e-4938.66UBX domain-containing protein [Actinidia rufa][more]
Match NameE-valueIdentityDescription
Q94HW27.2e-1921.84Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
Q9ZT941.2e-1324.14Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1DQX71.5e-8049.42uncharacterized protein LOC111022315 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A438GC621.5e-5138.53Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=2976... [more]
A0A7J0E8R31.5e-5143.00Uncharacterized protein OS=Actinidia rufa OX=165716 GN=Acr_02g0010880 PE=4 SV=1[more]
A0A6J1D6N71.7e-5046.52uncharacterized protein LOC111017438 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A7J0GPN08.2e-5038.66UBX domain-containing protein OS=Actinidia rufa OX=165716 GN=Acr_23g0011260 PE=4... [more]
Match NameE-valueIdentityDescription
AT1G21280.15.9e-0828.71CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); Ha... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 29..164
e-value: 2.7E-24
score: 85.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 203..219
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..243
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..202
NoneNo IPR availablePANTHERPTHR34222:SF48SUBFAMILY NOT NAMEDcoord: 2..336
NoneNo IPR availablePANTHERPTHR34222FAMILY NOT NAMEDcoord: 2..336

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032565.1Lag0032565.1mRNA