Lag0032557 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032557
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionGDSL esterase/lipase
Locationchr11: 34585385 .. 34589247 (+)
RNA-Seq ExpressionLag0032557
SyntenyLag0032557
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACACTTTATGCTTTTATTTGCACGACGCAATCAAGCTTTTCACATCATTATTTAACCAAAATGTGGAATGGAAGTGCTTTGAACTGAATAAGCATTCTTTGTACAACATGGAGTTTTCTAAACGCTACACTCTAATGTTCATTATTTTGTCCATGAATTTCTTACGATGTCTTTGTTTCACGAGCTTTGTGTTCGGAGATTCGCTCGTCGACGCTGGAAACAACGACTACATTTTCACCCTATCCAAGGCCGATTTGCCTCCTTACGGTATCGATTTCAAGCCCTCCGGTGGTCGCCCCACCGGAAGGTTCACCAACGGTCGAACTATATCAGATATTATCGGTAAGAAATGTTAAGAGAGCTCATTGAAGTTAAGGGTTGAAAACTAGGAGAGATAAACAAAATTTTCTCATTTTCTCCCTCCCTCCCTCTTTCTTAGTTGTTCTTTTCTATAATAATTGAATGATTGCAATATATACTTTTTTATTAAAAAAATCTTTTTTTTCATTTTTTTTTTGAAAAACTAGTGATGAGAAAACATACCATTTTTAAAAGAATCTTCACAAAAACAATAAAAAATGCACATATTTTTTTAGAAAAATTAATACAACTAAGCATGTCTTGTAGATTAAAGTATTTATGAAGTGTTTGAATCAGGTGAATATCTTGGAGCTGCCAAGTCATTTCCTCCTCCATTTCTAGCCCCAAATATTACAAAAGAAGATACAATATATAGAGGAATAAACTATGCTTCTGGAGCTTCTGGCATCTTGGATGAAACTGGTTTCTTATTTGTAAGTGCGCACTGACATAAAATATGTACTCTCGACCCAAAAAAATGGAAGTTAATAATTATATATATAAATATAATGTTTTGTATGAACAGTTGGGAAGGATTCCATTGGGGGAGCAAGTGAAGAATTTTGAGGAGAGTAGAGATTTCATGGTGAAGGTAAAGGGAGAGAATGAAACAATGGAATTGTTGAAGAATGCAATTTTCTCTTTAACAGTTGGATCCAATGATATAATCAACTATATTCAACCCTCCATTCCATTTTTCCAAAGAAACAAGCCTGCCCCAAGTGATTACTTGGATCATATGATCTCTAACTTGACAATACACCTCAAGGTAAAAGGAAAAAAATGTCTATTAAGATTTATAGTTTGAGTTTATTCTTGGATTTGTTATGTTAATAATGTATAGTTGAAAAGAAATAGTTTCTTATGGTATGTGCATATTAGTTTCCTAAAATCTTAAAATTTGAAATTTAGTCCTTTTAAATTTATTGAATCTACTTAAAAATGTTTTTATTGGTTATCCAAAGTTGATAGTTTCCTTGCCAAGCTTTCATGACTTCTTTTCCTTTGATAAAAAGAGAGATGATGAACCTTTTTTTTTTTTTTGGAAAAAATTAGTTAGTTAACATATGAACCCTTCTACTTATCACCTTTAAAAATCTTACAAACTAACAACAAAGGCTCGGTTTGATAACAATTTGGTTTTTGAAAATTATGTCTTCTCTCAATTTCCTTATAATTGGTTTTTATCATTGTTGTTGAAACATTTGAATTCTTAGTTACATTTTAAAAACAAAAACAAGTTCTCGAAAACTATTTTTTTAAGTTTTCAAAACTTGACTTGGTTTTTAAAAATATGAAAAGAACAAAGAAACTTGTGGGTGGGGTAGTGTTTATAGGCTTAATTTTCAAAAACCAAAAACCAAATGGTTATCAAACGAGACCAAAATATTTTTTTTTCTTCCTCTCGCTTTCTCTTTTTTTCTCTCCTACCAATCAAAACTTAAAAAGTGGTACTCGTGAATAATTACTAGATAAGCATTTTTCTTTTCCTTTTTCAAGAGTTTCTTTATATATATAGACACACGTGTTTGATAACAAAAAAAACACACAAAAAATCATTTGAATCCCAACTAATGCCAAACAAGCATACCCTCCACCATGAAAGGCCAAGGGAAAAAGTTAACCCACCATACACTTAAAAGAAGATGCCAATTAAATCTTTTGTCTGGTGAGAGACTTTAGTGATGGTACCAATTTTTGGACAATTATCTTGGCAAGACTAGAAGAATAAGCATTACTTTGGGCAAAGAAAATTTTAACAAAGTAGCTACTAAAGCTTACTTTTCCCACTTTTTATACAAAAAATTATCACCCAATATTGATGGGTCATCTCTTATAAAACAATTTATGGTCCATGGATTTAAAAGTATAAGACTTGTTAAGCTCAAGTTTATAAAAACTTATAAAAATTGTTTGATAACAATTTGGTTTTTGAAAATTAAGCTACGTAACTTGGTAATTTGTATATCTCTTTGTTAGGTTTGTGAGGTTATGATTTGAAAATGATGTACTTGATAATAAATGAAGTCGATTTTATAAGTCTAGATTTAGGTAGCTTGTGAGATTAATCGTCGGTGTTTATTGATGAAGCGGCTACACGAATTGGGAGCGCGAAAGTTCGTAGTAGTTGGAATTGGACCACTTGGGTGCATTCCATTTGTACGGGCAGTGTTTTTTGTAACAAGTGAGAGATGCTCAGAAGAAGTGAATCAGCTGATCAAAGCCTACAACTCTAGGCTAAGTAAAGTGGTTGATCAATTGAATCAAGAGTTTGGTCTCAAAACCATGTTCGTCTATGCTAATTCCTATGCTGTGTTCATGAAGATTATTGTCAATTATCGACAATATGGTTAGATTCCAAACCCCATCTCTTACTTGGATTTTTATTTGGGGTAAAATATTGCAGCTGGTTTTGATTCGGGTTTAAAAATTTTGAATTATCACTTTAGTCGTCCATATAGTTTGAGTTTGGTTTCATATTCATTTCTATATGGTTTTAAAAGTTTCGTGGAGGTCCATAGGTTAATAATTGTCACAAAATGTATGGCATATATTACAAGTCGTCCATTAAATGTAACAAGGAGAAAAGCAATGAATTGACCATTAGGTATGAGTTTAAGTTAAATAGAATGAAAATATAGGGTTGGTTTTGTATTAATGTTTGAATTTTTTCCAAATAAAGAAAATTTGGTGGGATTTAGGTAGGAAGAAAATAGGTTGTGCAGAATAAGGGTGAGTCTTTATTGCACATTTTAAAAAGATTCTAATCCCCAAACTCACCCCTTTTCTCTTTTTTCCATGTCAATATATATGTATATATAATATCTAACCAAACCATTGGGACTATATATAGGAATCAAATTTATGGAAAAATAAATATTATAGAAAACCTAACAAAAAACTTTGGGACTAATATATATAGGAATTCAAATTTAAATGAAGAAGCTAAGATCTTAGAGAATTGAATATGGTGATTTGACCTAAGAAAAATTGAACAGGTAAAAATAATGTCTCTCAAACGATTCAGGTAGGTGAAAATTTCTAGTACTATGATTCATACTACCTCCATTCATGGTTTGTCATGAAAGAAGTCATTTATTGCATTGCATAGACATGTGTTTCTTTCTCAAATCATTTCAAGTTTGTATGTTTCAAAAACACTTATTGTACATTTTAAATTAACTTTCTTTCACCCTAATTGATTTTCTTTTTCCTTTTCTTTTGTGGTGAAAATATATGTTTTTTTTAACACATGATAGGGTTTGTGAATGCAAATCAACCATGTTGTGGTGGATACTTTCCTCCTTTCATTTGCTACAAAGACCAAAACCAAGACAAAAGCTCTTCTCTATGTGATGATAGATCAAAATATGTGTTTTGGGATGCTTATCACCCAACAGAAGCTGCTAATATCATCATTGCCCAAGAGCTGCTAGATGGAGATGAAACCATAAGTTCTCCCATCAACATTCGTCAGCTCTACGCGTATCGTTTATAA

mRNA sequence

ATGCACACTTTATGCTTTTATTTGCACGACGCAATCAAGCTTTTCACATCATTATTTAACCAAAATGTGGAATGGAAGTGCTTTGAACTGAATAAGCATTCTTTGTACAACATGGAGTTTTCTAAACGCTACACTCTAATGTTCATTATTTTGTCCATGAATTTCTTACGATGTCTTTGTTTCACGAGCTTTGTGTTCGGAGATTCGCTCGTCGACGCTGGAAACAACGACTACATTTTCACCCTATCCAAGGCCGATTTGCCTCCTTACGGTATCGATTTCAAGCCCTCCGGTGGTCGCCCCACCGGAAGGTTCACCAACGGTCGAACTATATCAGATATTATCGGTGAATATCTTGGAGCTGCCAAGTCATTTCCTCCTCCATTTCTAGCCCCAAATATTACAAAAGAAGATACAATATATAGAGGAATAAACTATGCTTCTGGAGCTTCTGGCATCTTGGATGAAACTGGTTTCTTATTTTTGGGAAGGATTCCATTGGGGGAGCAAGTGAAGAATTTTGAGGAGAGTAGAGATTTCATGGTGAAGGTAAAGGGAGAGAATGAAACAATGGAATTGTTGAAGAATGCAATTTTCTCTTTAACAGTTGGATCCAATGATATAATCAACTATATTCAACCCTCCATTCCATTTTTCCAAAGAAACAAGCCTGCCCCAAGTGATTACTTGGATCATATGATCTCTAACTTGACAATACACCTCAAGCGGCTACACGAATTGGGAGCGCGAAAGTTCGTAGTAGTTGGAATTGGACCACTTGGGTGCATTCCATTTGTACGGGCAGTGTTTTTTGTAACAAGTGAGAGATGCTCAGAAGAAGTGAATCAGCTGATCAAAGCCTACAACTCTAGGCTAAGTAAAGTGGTTGATCAATTGAATCAAGAGTTTGGTCTCAAAACCATGTTCGTCTATGCTAATTCCTATGCTGTGTTCATGAAGATTATTGTCAATTATCGACAATATGGGTTTGTGAATGCAAATCAACCATGTTGTGGTGGATACTTTCCTCCTTTCATTTGCTACAAAGACCAAAACCAAGACAAAAGCTCTTCTCTATGTGATGATAGATCAAAATATGTGTTTTGGGATGCTTATCACCCAACAGAAGCTGCTAATATCATCATTGCCCAAGAGCTGCTAGATGGAGATGAAACCATAAGTTCTCCCATCAACATTCGTCAGCTCTACGCGTATCGTTTATAA

Coding sequence (CDS)

ATGCACACTTTATGCTTTTATTTGCACGACGCAATCAAGCTTTTCACATCATTATTTAACCAAAATGTGGAATGGAAGTGCTTTGAACTGAATAAGCATTCTTTGTACAACATGGAGTTTTCTAAACGCTACACTCTAATGTTCATTATTTTGTCCATGAATTTCTTACGATGTCTTTGTTTCACGAGCTTTGTGTTCGGAGATTCGCTCGTCGACGCTGGAAACAACGACTACATTTTCACCCTATCCAAGGCCGATTTGCCTCCTTACGGTATCGATTTCAAGCCCTCCGGTGGTCGCCCCACCGGAAGGTTCACCAACGGTCGAACTATATCAGATATTATCGGTGAATATCTTGGAGCTGCCAAGTCATTTCCTCCTCCATTTCTAGCCCCAAATATTACAAAAGAAGATACAATATATAGAGGAATAAACTATGCTTCTGGAGCTTCTGGCATCTTGGATGAAACTGGTTTCTTATTTTTGGGAAGGATTCCATTGGGGGAGCAAGTGAAGAATTTTGAGGAGAGTAGAGATTTCATGGTGAAGGTAAAGGGAGAGAATGAAACAATGGAATTGTTGAAGAATGCAATTTTCTCTTTAACAGTTGGATCCAATGATATAATCAACTATATTCAACCCTCCATTCCATTTTTCCAAAGAAACAAGCCTGCCCCAAGTGATTACTTGGATCATATGATCTCTAACTTGACAATACACCTCAAGCGGCTACACGAATTGGGAGCGCGAAAGTTCGTAGTAGTTGGAATTGGACCACTTGGGTGCATTCCATTTGTACGGGCAGTGTTTTTTGTAACAAGTGAGAGATGCTCAGAAGAAGTGAATCAGCTGATCAAAGCCTACAACTCTAGGCTAAGTAAAGTGGTTGATCAATTGAATCAAGAGTTTGGTCTCAAAACCATGTTCGTCTATGCTAATTCCTATGCTGTGTTCATGAAGATTATTGTCAATTATCGACAATATGGGTTTGTGAATGCAAATCAACCATGTTGTGGTGGATACTTTCCTCCTTTCATTTGCTACAAAGACCAAAACCAAGACAAAAGCTCTTCTCTATGTGATGATAGATCAAAATATGTGTTTTGGGATGCTTATCACCCAACAGAAGCTGCTAATATCATCATTGCCCAAGAGCTGCTAGATGGAGATGAAACCATAAGTTCTCCCATCAACATTCGTCAGCTCTACGCGTATCGTTTATAA

Protein sequence

MHTLCFYLHDAIKLFTSLFNQNVEWKCFELNKHSLYNMEFSKRYTLMFIILSMNFLRCLCFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGRPTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDTIYRGINYASGASGILDETGFLFLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPSIPFFQRNKPAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSEEVNQLIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQPCCGGYFPPFICYKDQNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSPINIRQLYAYRL
Homology
BLAST of Lag0032557 vs. NCBI nr
Match: XP_004143942.3 (GDSL esterase/lipase At5g41890 [Cucumis sativus])

HSP 1 Score: 637.5 bits (1643), Expect = 7.9e-179
Identity = 313/372 (84.14%), Postives = 335/372 (90.05%), Query Frame = 0

Query: 38  MEFSKRYTLMFIILSMNFLR-CLCFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKP 97
           MEFSK Y +M I+  +NF     CFT+FVFGDSLVDAGNNDYIFTLSKAD PPYGIDFKP
Sbjct: 6   MEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP 65

Query: 98  SGGRPTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDT-IYRGINYASGASGILD 157
           SGG+PTGRFTNGRTISDIIGEYLG AKSFPPPFLAP  T+ DT IY+GINYASGASGILD
Sbjct: 66  SGGQPTGRFTNGRTISDIIGEYLG-AKSFPPPFLAPISTQSDTIIYKGINYASGASGILD 125

Query: 158 ETGFLFLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPS 217
           ETG LFLGRI L EQVKNFEESR+ MVKVKGENETME+LKN+IFSLTVGSNDIINYIQPS
Sbjct: 126 ETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPS 185

Query: 218 IPFFQRNKPAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSE 277
           IPF Q NKP+PSDYLDHMISNLT+HLKRLH LGARKFVVVG+GPLGCIPFVRA+ FVT+E
Sbjct: 186 IPFLQTNKPSPSDYLDHMISNLTVHLKRLHALGARKFVVVGVGPLGCIPFVRAIHFVTNE 245

Query: 278 RCSEEVNQLIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQ 337
           +C EEVNQLI+ YN RL+  VDQLN EFGL TMF+YANSYAVF KIIVNYRQYGFVNA Q
Sbjct: 246 KCLEEVNQLIETYNFRLNGAVDQLNLEFGLSTMFIYANSYAVFTKIIVNYRQYGFVNAKQ 305

Query: 338 PCCGGYFPPFICYKDQNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISS 397
           PCC GYFPPFICYKDQNQ  SS LC+DRSKYVFWDAYHPTEAANIIIA+ELLDGDETI+S
Sbjct: 306 PCCVGYFPPFICYKDQNQSSSSFLCEDRSKYVFWDAYHPTEAANIIIAKELLDGDETITS 365

Query: 398 PINIRQLYAYRL 408
           PINIRQLYAYRL
Sbjct: 366 PINIRQLYAYRL 376

BLAST of Lag0032557 vs. NCBI nr
Match: XP_022158401.1 (GDSL esterase/lipase At5g41890 [Momordica charantia])

HSP 1 Score: 634.0 bits (1634), Expect = 8.7e-178
Identity = 309/371 (83.29%), Postives = 338/371 (91.11%), Query Frame = 0

Query: 38  MEFSKRY--TLMFIILSMNFLRCLCFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFK 97
           MEFS+ +  T+M ++ +MNFL CLC T+FVFGDSLVD GNNDYIFTLS+AD PPYGIDFK
Sbjct: 1   MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK 60

Query: 98  PSGGRPTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDTIYRGINYASGASGILD 157
           PSGG+PTGRFTNGRTISDII EYLG AKSFPPPFLAPN T+ DT+YRGINYASGASGILD
Sbjct: 61  PSGGQPTGRFTNGRTISDIIDEYLG-AKSFPPPFLAPN-TEVDTMYRGINYASGASGILD 120

Query: 158 ETGFLFLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPS 217
           ETG LFLGRIPL EQVKNFEESR +MVKVKGENETME+LKNAIFSLTVGSNDIINYIQPS
Sbjct: 121 ETGLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPS 180

Query: 218 IPFFQRNKPAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSE 277
           IP FQ NK +PSDYLDHMISN+TI LKRLH +GARK VVVG+GPLGCIPFVR + F  ++
Sbjct: 181 IPIFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPND 240

Query: 278 RCSEEVNQLIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQ 337
            CSEEVNQLI+AYN RLS+VVDQLNQEF  KTMFVYANSYAVFM+II NY+QYGFV+A Q
Sbjct: 241 ECSEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQ 300

Query: 338 PCCGGYFPPFICYKDQNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISS 397
           PCCGGYFPPF+CYK QNQ++SSSLCDDRSKYVFWDAYHPTEAANIIIA+ELLDGDETISS
Sbjct: 301 PCCGGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISS 360

Query: 398 PINIRQLYAYR 407
           PINIRQLYAYR
Sbjct: 361 PINIRQLYAYR 369

BLAST of Lag0032557 vs. NCBI nr
Match: XP_022977773.1 (GDSL esterase/lipase At5g41890 [Cucurbita maxima])

HSP 1 Score: 632.5 bits (1630), Expect = 2.5e-177
Identity = 312/375 (83.20%), Postives = 339/375 (90.40%), Query Frame = 0

Query: 38  MEFSK-RYTLMFIILSMNFLRCLCFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKP 97
           M+FSK  Y +MF++ S+NF   LCFT+FVFGDSLVDAGNNDYIFTLSKAD PPYGIDFKP
Sbjct: 1   MKFSKYSYIMMFVVFSINFFPGLCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP 60

Query: 98  SGGRPTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDTIYRGINYASGASGILDE 157
           SGGRPTGRFTNGRTISDIIGEYLG A+SFPPPFLAPN T+ DTIY GINYASGA+GILDE
Sbjct: 61  SGGRPTGRFTNGRTISDIIGEYLG-AESFPPPFLAPN-TEADTIYTGINYASGAAGILDE 120

Query: 158 TGFLFLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPSI 217
           TGFLFLGRIPL EQVK+FEESRD++VKVKGENETM+LLK AIFSLT GSNDIINY QPSI
Sbjct: 121 TGFLFLGRIPLREQVKHFEESRDYIVKVKGENETMKLLKEAIFSLTFGSNDIINYFQPSI 180

Query: 218 PFFQRNKPAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSER 277
           PFFQ NK APSDYLDHMISNLTIHLK LH+LGARKFVVVG+GPLGCIPFVRAV FV  E+
Sbjct: 181 PFFQSNKVAPSDYLDHMISNLTIHLKELHDLGARKFVVVGVGPLGCIPFVRAVQFVRGEK 240

Query: 278 CSEEVNQLIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQP 337
           CSEEVN++I+AYN RLS +V +LNQEFG KTMFVYANSYAVFMKIIVNY+QYGFVNA QP
Sbjct: 241 CSEEVNEVIEAYNVRLSGLVGELNQEFGPKTMFVYANSYAVFMKIIVNYQQYGFVNAKQP 300

Query: 338 CCGGYFPPFICY-----KDQNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDE 397
           CCG YFPPFICY     +DQN+  SS LCDDRSKYVFWDAYHPTEAANIIIA+ELLDGDE
Sbjct: 301 CCGAYFPPFICYNKGQSQDQNRSSSSFLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDE 360

Query: 398 TISSPINIRQLYAYR 407
           TIS+PINIRQL+AYR
Sbjct: 361 TISAPINIRQLHAYR 373

BLAST of Lag0032557 vs. NCBI nr
Match: XP_008437733.1 (PREDICTED: GDSL esterase/lipase At5g41890 [Cucumis melo])

HSP 1 Score: 630.9 bits (1626), Expect = 7.4e-177
Identity = 313/373 (83.91%), Postives = 338/373 (90.62%), Query Frame = 0

Query: 38  MEFSKRYTLMFIILSMNFL-RCLCFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKP 97
           M FSK Y +M I+ S+NF    LCFT+FVFGDSLVDAGNNDYIFTLSKA+ PPYGIDFKP
Sbjct: 1   MVFSKSYVVMVIVFSINFFDGGLCFTTFVFGDSLVDAGNNDYIFTLSKANSPPYGIDFKP 60

Query: 98  SGGRPTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDT-IYRGINYASGASGILD 157
           SGG+PTGRFTNGRTISDIIGEYLG AKSFPPPFLAP  T+ DT I++GINYASGASGILD
Sbjct: 61  SGGQPTGRFTNGRTISDIIGEYLG-AKSFPPPFLAPISTQSDTIIHKGINYASGASGILD 120

Query: 158 ETGFLFLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPS 217
           ETG LFLGRI L EQVKNFEESR+ MVK+KGENETME+LKN+IFSLTVGSNDIINYIQPS
Sbjct: 121 ETGLLFLGRISLREQVKNFEESRNEMVKLKGENETMEVLKNSIFSLTVGSNDIINYIQPS 180

Query: 218 IPFFQRNKPAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSE 277
           IPFFQ NKP+PSDYLDHMISNLT+HLKRLH LGARKFVVVG+GPLGCIPFVRA+ FVT+E
Sbjct: 181 IPFFQTNKPSPSDYLDHMISNLTVHLKRLHALGARKFVVVGVGPLGCIPFVRAIHFVTNE 240

Query: 278 RCSEEVNQLIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQ 337
           +C EEVN+LI+ YN +LS VVDQLN EFGL TMFVYANSYAVF KIIVNYRQYGFVNA Q
Sbjct: 241 KCLEEVNRLIETYNFKLSGVVDQLNLEFGLNTMFVYANSYAVFTKIIVNYRQYGFVNAKQ 300

Query: 338 PCCGGYFPPFICYKD-QNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETIS 397
           PCC GYFPPFICYKD QNQ  +S LCDDRSKYVFWDAYHPTEAANIIIA+ELLDGDETI+
Sbjct: 301 PCCVGYFPPFICYKDRQNQSSTSFLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETIT 360

Query: 398 SPINIRQLYAYRL 408
           SPINIRQLYAYRL
Sbjct: 361 SPINIRQLYAYRL 372

BLAST of Lag0032557 vs. NCBI nr
Match: XP_038907274.1 (GDSL esterase/lipase At5g41890 [Benincasa hispida])

HSP 1 Score: 628.2 bits (1619), Expect = 4.8e-176
Identity = 310/370 (83.78%), Postives = 336/370 (90.81%), Query Frame = 0

Query: 42  KRYTLMFIILSMNFLRCL-CFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGR 101
           K Y + FI+  +NF   + CFT+FVFGDSLVD GNNDYIFTLSKAD PPYGIDFKPSGG+
Sbjct: 53  KSYVVTFIVFFINFFGGVHCFTTFVFGDSLVDVGNNDYIFTLSKADSPPYGIDFKPSGGQ 112

Query: 102 PTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDTIYRGINYASGASGILDETGFL 161
           PTGRFTNGRTISDIIGEYLG AKSFP PFLAP+  + DTIY GINYASGASGILDETG L
Sbjct: 113 PTGRFTNGRTISDIIGEYLG-AKSFPLPFLAPS-KEADTIYNGINYASGASGILDETGLL 172

Query: 162 FLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPSIPFFQ 221
           FLGRIPL EQVKNFE SRD+MVKVKGENETME+LKNAIFSLTVGSNDIINYIQPSIPFFQ
Sbjct: 173 FLGRIPLREQVKNFEASRDYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIPFFQ 232

Query: 222 RNKPAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSEE 281
           RNK +PSDYLDHM+SNLTIHLK+LH+LGARKFVVVG+GPLGCIPFVRAV FVT+E+CSEE
Sbjct: 233 RNKASPSDYLDHMVSNLTIHLKQLHKLGARKFVVVGVGPLGCIPFVRAVHFVTNEKCSEE 292

Query: 282 VNQLIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQPCCGG 341
           VN+LI+AYN +L++VVDQLN EFG  TMFVYANSY VF KIIVNY+QYGFVNA QPCCGG
Sbjct: 293 VNRLIEAYNFKLNEVVDQLNLEFGPNTMFVYANSYTVFTKIIVNYQQYGFVNAKQPCCGG 352

Query: 342 YFPPFICYKDQNQDK----SSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSP 401
           YFPPFICYKDQNQD+    SS LCDDRSKYVFWDAYHPTEAANIIIA+ELLDGDETISSP
Sbjct: 353 YFPPFICYKDQNQDQNQSSSSFLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSP 412

Query: 402 INIRQLYAYR 407
           INIRQL+AYR
Sbjct: 413 INIRQLHAYR 420

BLAST of Lag0032557 vs. ExPASy Swiss-Prot
Match: Q9FJ25 (GDSL esterase/lipase At5g41890 OS=Arabidopsis thaliana OX=3702 GN=At5g41890 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 1.8e-133
Identity = 224/346 (64.74%), Postives = 284/346 (82.08%), Query Frame = 0

Query: 61  FTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGRPTGRFTNGRTISDIIGEYLG 120
           FT+F+FGDSLVD GNN+YIFTLSKAD  PYGIDF PS G+PTGRFTNGRTISDI+GE LG
Sbjct: 24  FTNFIFGDSLVDVGNNNYIFTLSKADSSPYGIDFAPSNGQPTGRFTNGRTISDIVGEALG 83

Query: 121 AAKSFPPPFLAPNITKEDTIYRGINYASGASGILDETGFLFLGRIPLGEQVKNFEESRDF 180
            AKS PPP+L PN T+ +TI  GINYASGA+GILD+TG LF+GR+PL EQV NFE+SR++
Sbjct: 84  -AKSPPPPYLEPN-TEANTIRNGINYASGAAGILDDTGLLFIGRVPLREQVSNFEKSREY 143

Query: 181 MVKVKGENETMELLKNAIFSLTVGSNDIINYIQPSIPFFQRNKPAPSDYL-DHMISNLTI 240
           MV+V GEN T E+LKNA+F++T+GSNDI+NYIQPSIPFF ++K  P+D L D M+ +LT 
Sbjct: 144 MVRVIGENGTKEMLKNAMFTITIGSNDILNYIQPSIPFFSQDK-LPTDVLQDSMVLHLTT 203

Query: 241 HLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSEEVNQLIKAYNSRLSKVVDQL 300
           HLKRLH+LG RKFVVVG+GPLGCIPF RA+  + + +CSE+VNQ+++ YN +L   +  L
Sbjct: 204 HLKRLHQLGGRKFVVVGVGPLGCIPFARALNLIPAGKCSEQVNQVVRGYNMKLIHSLKTL 263

Query: 301 NQEF---GLKTMFVYANSYAVFMKIIVNYRQYGFVNANQPCCGGYFPPFICYKDQNQDKS 360
           N E       T FVYANSY +F+K+++NY+ +G  NA++PCCGGYFPPF C+K  NQ+ S
Sbjct: 264 NNELRSEDYNTTFVYANSYDLFLKLVLNYQLFGLKNADKPCCGGYFPPFACFKGPNQNSS 323

Query: 361 SSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSPINIRQL 403
            + C+DRSK+VFWDAYHPTEAAN+I+A+ LLDGD+T+++P NIR L
Sbjct: 324 QAACEDRSKFVFWDAYHPTEAANLIVAKALLDGDQTVATPFNIRYL 366

BLAST of Lag0032557 vs. ExPASy Swiss-Prot
Match: O80470 (GDSL esterase/lipase At2g23540 OS=Arabidopsis thaliana OX=3702 GN=At2g23540 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 3.2e-82
Identity = 157/345 (45.51%), Postives = 229/345 (66.38%), Query Frame = 0

Query: 63  SFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGRPTGRFTNGRTISDIIGEYLGAA 122
           SF+FGDSLVDAGNN+Y+ TLS+A++ P GIDFK SGG PTGRFTNGRTI DI+GE LG+A
Sbjct: 49  SFIFGDSLVDAGNNNYLSTLSRANMKPNGIDFKASGGTPTGRFTNGRTIGDIVGEELGSA 108

Query: 123 KSFPPPFLAPNITKEDTIYRGINYASGASGILDETGFLFLGRIPLGEQVKNFEESRDFMV 182
            ++  PFLAP+  K   +  G+NYASG  GI++ TG +F+ R+ +  QV  F  +R    
Sbjct: 109 -NYAIPFLAPD-AKGKALLAGVNYASGGGGIMNATGRIFVNRLGMDVQVDFFNTTRKQFD 168

Query: 183 KVKGENETME-LLKNAIFSLTVGSNDII-NYIQPSIPFFQRNKPAPSDYLDHMISNLTIH 242
            + G+ +  + + K +IFS+T+G+ND + NY+ P +    R    P D++  M+ +L   
Sbjct: 169 DLLGKEKAKDYIAKKSIFSITIGANDFLNNYLFPLLSVGTRFTQTPDDFIGDMLEHLRDQ 228

Query: 243 LKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSEEVNQLIKAYNSRLSKVVDQLN 302
           L RL++L ARKFV+  +GP+GCIP+ + +  +    C +  N+L   YN RL  ++++LN
Sbjct: 229 LTRLYQLDARKFVIGNVGPIGCIPYQKTINQLDENECVDLANKLANQYNVRLKSLLEELN 288

Query: 303 QEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQPCC--GGYFPPFI-CYKDQNQDKSS 362
           ++     MFV+AN Y + M++I NY +YGF +A + CC  GG +   I C        +S
Sbjct: 289 KKLP-GAMFVHANVYDLVMELITNYDKYGFKSATKACCGNGGQYAGIIPC------GPTS 348

Query: 363 SLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSPINIRQL 403
           SLC++R KYVFWD YHP+EAAN+IIA++LL GD  + SP+N+ +L
Sbjct: 349 SLCEERDKYVFWDPYHPSEAANVIIAKQLLYGDVKVISPVNLSKL 384

BLAST of Lag0032557 vs. ExPASy Swiss-Prot
Match: Q9M2R9 (GDSL esterase/lipase At3g50400 OS=Arabidopsis thaliana OX=3702 GN=At3g50400 PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 5.5e-82
Identity = 161/348 (46.26%), Postives = 227/348 (65.23%), Query Frame = 0

Query: 63  SFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGRPTGRFTNGRTISDIIGEYLGAA 122
           SFVFGDSLVDAGNN+Y+ TLS+A+ PP GIDFKPS G PTGRFTNGRTI+DI+GE LG  
Sbjct: 35  SFVFGDSLVDAGNNNYLQTLSRANSPPNGIDFKPSRGNPTGRFTNGRTIADIVGEKLG-Q 94

Query: 123 KSFPPPFLAPNITKEDTIYRGINYASGASGILDETGFLFLGRIPLGEQVKNFEESRDFMV 182
           +S+  P+LAPN + E  +  G+NYASG  GIL+ TG +F+ R+ +  QV  F  +R    
Sbjct: 95  QSYAVPYLAPNASGE-ALLNGVNYASGGGGILNATGSVFVNRLGMDIQVDYFTNTRKQFD 154

Query: 183 KVKGENETMELL-KNAIFSLTVGSNDII-NYIQPSIPFFQRNKPAPSDYLDHMISNLTIH 242
           K+ G+++  + + K ++FS+ +GSND + NY+ P +    R    P  ++D MIS+L   
Sbjct: 155 KLLGQDKARDYIRKRSLFSVVIGSNDFLNNYLVPFVAAQARLTQTPETFVDDMISHLRNQ 214

Query: 243 LKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSEEVNQLIKAYNSRLSKVVDQLN 302
           LKRL+++ ARKFVV  + P+GCIP+ +++  +  ++C +  N+L   YN+RL  ++    
Sbjct: 215 LKRLYDMDARKFVVGNVAPIGCIPYQKSINQLNDKQCVDLANKLAIQYNARLKDLLTVEL 274

Query: 303 QEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQPCC------GGYFPPFICYKDQNQD 362
           ++      FVYAN Y +FM +IVN++ YGF  A++ CC       G  P   C       
Sbjct: 275 KDSLKDAHFVYANVYDLFMDLIVNFKDYGFRTASEACCETRGRLAGILP---C------G 334

Query: 363 KSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSPINIRQL 403
            +SSLC DRSK+VFWDAYHPTEAAN++IA +LL GD    +P N+  L
Sbjct: 335 PTSSLCTDRSKHVFWDAYHPTEAANLLIADKLLYGDSKFVTPFNLLHL 371

BLAST of Lag0032557 vs. ExPASy Swiss-Prot
Match: O23470 (GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3 SV=2)

HSP 1 Score: 306.2 bits (783), Expect = 5.5e-82
Identity = 177/372 (47.58%), Postives = 225/372 (60.48%), Query Frame = 0

Query: 44  YTLMFIILSMNFLRCLCF------TSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPS 103
           +    I+LS+ F   +C        +FVFGDSLVDAGNN+Y+ TLSKA+  P GIDF   
Sbjct: 6   FLCQIIVLSVLFFSEVCLAGKKIPANFVFGDSLVDAGNNNYLATLSKANYVPNGIDF--- 65

Query: 104 GGRPTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDTIYRGINYASGASGILDET 163
            G PTGRFTNGRTI DI+ + LG +    PP+LAP  T    I  G+NYASG SGIL+ T
Sbjct: 66  -GSPTGRFTNGRTIVDIVYQALG-SDELTPPYLAPT-TSGSLILNGVNYASGGSGILNST 125

Query: 164 GFLFLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDII-NYIQPSI 223
           G LF  RI +  Q+ NF  +R  ++   GE+E  +L ++AIFS+T GSND+I NY  P I
Sbjct: 126 GKLFGERINVDAQLDNFATTRQDIISWIGESEAAKLFRSAIFSVTTGSNDLINNYFTPVI 185

Query: 224 PFFQRNKPAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSER 283
              QR   AP  ++D MIS   + L RL++LGARK VV+ IGP+GCIPF R         
Sbjct: 186 STLQRKVVAPEVFVDTMISKFRLQLTRLYQLGARKIVVINIGPIGCIPFERESDPAAGNN 245

Query: 284 CSEEVNQLIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQP 343
           C  E N++ + YN +L  +V++LN+     + FVY + + +   II NY  YGF +   P
Sbjct: 246 CLAEPNEVAQMYNLKLKTLVEELNKNL-QGSRFVYGDVFRIVDDIIQNYSSYGFESEKIP 305

Query: 344 CC------GGYFPPFICYKDQNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGD 403
           CC      GG  P   C         S +C DRSKYVFWD YHPTEAANIIIA+ LL GD
Sbjct: 306 CCSLVGKVGGLIP---C------GPPSKVCMDRSKYVFWDPYHPTEAANIIIARRLLSGD 361

BLAST of Lag0032557 vs. ExPASy Swiss-Prot
Match: Q9SF78 (GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.2e-62
Identity = 138/346 (39.88%), Postives = 200/346 (57.80%), Query Frame = 0

Query: 64  FVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGRPTGRFTNGRTISDIIGEYLGAAK 123
           FVFGDSL+D GNN+ I + +KA+  PYGIDF    G PTGRF NG T+ D I + LG   
Sbjct: 57  FVFGDSLIDNGNNNNIPSFAKANYFPYGIDF---NGGPTGRFCNGLTMVDGIAQLLGL-- 116

Query: 124 SFPPPFLAPNITKEDTIYRGINYASGASGILDETGFLFLGRIPLGEQVKNFEESRDFMV- 183
              P   A +    D + RG+NYAS A+GIL +TG  F+GRIP  +Q+ NFE + D +  
Sbjct: 117 ---PLIPAYSEATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVAS 176

Query: 184 KVKGENETMELLKNAIFSLTVGSNDII-NYIQPSIPFFQRNKPAPSDYLDHMISNLTIHL 243
           K  G     + +  ++F + +GSND + NY+ P+ P   RN+     + D ++ + T  L
Sbjct: 177 KSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFP--TRNQYNSQQFGDLLVQHYTDQL 236

Query: 244 KRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSEEVNQLIKAYNSRLSKVVDQLNQ 303
            RL+ LG RKFVV G+G +GCIP + A       +CSEEVNQL+  +N+ +  ++  LNQ
Sbjct: 237 TRLYNLGGRKFVVAGLGRMGCIPSILAQ--GNDGKCSEEVNQLVLPFNTNVKTMISNLNQ 296

Query: 304 EFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQPCCGGYFPPFICYKDQNQDKSSSL-- 363
                  F+Y +   +F  I+ N   YG    ++ CCG           +N+ + + L  
Sbjct: 297 NLP-DAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCG---------IGKNRGQITCLPF 356

Query: 364 ---CDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSPINIRQL 403
              C +R +YVFWDA+HPTE  N+I+A++   GD T++ PINI+QL
Sbjct: 357 ETPCPNRDQYVFWDAFHPTEKVNLIMAKKAFAGDRTVAYPINIQQL 380

BLAST of Lag0032557 vs. ExPASy TrEMBL
Match: A0A0A0KM97 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148500 PE=3 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 3.8e-179
Identity = 313/372 (84.14%), Postives = 335/372 (90.05%), Query Frame = 0

Query: 38  MEFSKRYTLMFIILSMNFLR-CLCFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKP 97
           MEFSK Y +M I+  +NF     CFT+FVFGDSLVDAGNNDYIFTLSKAD PPYGIDFKP
Sbjct: 1   MEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP 60

Query: 98  SGGRPTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDT-IYRGINYASGASGILD 157
           SGG+PTGRFTNGRTISDIIGEYLG AKSFPPPFLAP  T+ DT IY+GINYASGASGILD
Sbjct: 61  SGGQPTGRFTNGRTISDIIGEYLG-AKSFPPPFLAPISTQSDTIIYKGINYASGASGILD 120

Query: 158 ETGFLFLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPS 217
           ETG LFLGRI L EQVKNFEESR+ MVKVKGENETME+LKN+IFSLTVGSNDIINYIQPS
Sbjct: 121 ETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPS 180

Query: 218 IPFFQRNKPAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSE 277
           IPF Q NKP+PSDYLDHMISNLT+HLKRLH LGARKFVVVG+GPLGCIPFVRA+ FVT+E
Sbjct: 181 IPFLQTNKPSPSDYLDHMISNLTVHLKRLHALGARKFVVVGVGPLGCIPFVRAIHFVTNE 240

Query: 278 RCSEEVNQLIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQ 337
           +C EEVNQLI+ YN RL+  VDQLN EFGL TMF+YANSYAVF KIIVNYRQYGFVNA Q
Sbjct: 241 KCLEEVNQLIETYNFRLNGAVDQLNLEFGLSTMFIYANSYAVFTKIIVNYRQYGFVNAKQ 300

Query: 338 PCCGGYFPPFICYKDQNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISS 397
           PCC GYFPPFICYKDQNQ  SS LC+DRSKYVFWDAYHPTEAANIIIA+ELLDGDETI+S
Sbjct: 301 PCCVGYFPPFICYKDQNQSSSSFLCEDRSKYVFWDAYHPTEAANIIIAKELLDGDETITS 360

Query: 398 PINIRQLYAYRL 408
           PINIRQLYAYRL
Sbjct: 361 PINIRQLYAYRL 371

BLAST of Lag0032557 vs. ExPASy TrEMBL
Match: A0A6J1E0T3 (GDSL esterase/lipase At5g41890 OS=Momordica charantia OX=3673 GN=LOC111024890 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.2e-178
Identity = 309/371 (83.29%), Postives = 338/371 (91.11%), Query Frame = 0

Query: 38  MEFSKRY--TLMFIILSMNFLRCLCFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFK 97
           MEFS+ +  T+M ++ +MNFL CLC T+FVFGDSLVD GNNDYIFTLS+AD PPYGIDFK
Sbjct: 1   MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK 60

Query: 98  PSGGRPTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDTIYRGINYASGASGILD 157
           PSGG+PTGRFTNGRTISDII EYLG AKSFPPPFLAPN T+ DT+YRGINYASGASGILD
Sbjct: 61  PSGGQPTGRFTNGRTISDIIDEYLG-AKSFPPPFLAPN-TEVDTMYRGINYASGASGILD 120

Query: 158 ETGFLFLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPS 217
           ETG LFLGRIPL EQVKNFEESR +MVKVKGENETME+LKNAIFSLTVGSNDIINYIQPS
Sbjct: 121 ETGLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPS 180

Query: 218 IPFFQRNKPAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSE 277
           IP FQ NK +PSDYLDHMISN+TI LKRLH +GARK VVVG+GPLGCIPFVR + F  ++
Sbjct: 181 IPIFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPND 240

Query: 278 RCSEEVNQLIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQ 337
            CSEEVNQLI+AYN RLS+VVDQLNQEF  KTMFVYANSYAVFM+II NY+QYGFV+A Q
Sbjct: 241 ECSEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQ 300

Query: 338 PCCGGYFPPFICYKDQNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISS 397
           PCCGGYFPPF+CYK QNQ++SSSLCDDRSKYVFWDAYHPTEAANIIIA+ELLDGDETISS
Sbjct: 301 PCCGGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISS 360

Query: 398 PINIRQLYAYR 407
           PINIRQLYAYR
Sbjct: 361 PINIRQLYAYR 369

BLAST of Lag0032557 vs. ExPASy TrEMBL
Match: A0A6J1IR23 (GDSL esterase/lipase At5g41890 OS=Cucurbita maxima OX=3661 GN=LOC111477974 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.2e-177
Identity = 312/375 (83.20%), Postives = 339/375 (90.40%), Query Frame = 0

Query: 38  MEFSK-RYTLMFIILSMNFLRCLCFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKP 97
           M+FSK  Y +MF++ S+NF   LCFT+FVFGDSLVDAGNNDYIFTLSKAD PPYGIDFKP
Sbjct: 1   MKFSKYSYIMMFVVFSINFFPGLCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP 60

Query: 98  SGGRPTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDTIYRGINYASGASGILDE 157
           SGGRPTGRFTNGRTISDIIGEYLG A+SFPPPFLAPN T+ DTIY GINYASGA+GILDE
Sbjct: 61  SGGRPTGRFTNGRTISDIIGEYLG-AESFPPPFLAPN-TEADTIYTGINYASGAAGILDE 120

Query: 158 TGFLFLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPSI 217
           TGFLFLGRIPL EQVK+FEESRD++VKVKGENETM+LLK AIFSLT GSNDIINY QPSI
Sbjct: 121 TGFLFLGRIPLREQVKHFEESRDYIVKVKGENETMKLLKEAIFSLTFGSNDIINYFQPSI 180

Query: 218 PFFQRNKPAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSER 277
           PFFQ NK APSDYLDHMISNLTIHLK LH+LGARKFVVVG+GPLGCIPFVRAV FV  E+
Sbjct: 181 PFFQSNKVAPSDYLDHMISNLTIHLKELHDLGARKFVVVGVGPLGCIPFVRAVQFVRGEK 240

Query: 278 CSEEVNQLIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQP 337
           CSEEVN++I+AYN RLS +V +LNQEFG KTMFVYANSYAVFMKIIVNY+QYGFVNA QP
Sbjct: 241 CSEEVNEVIEAYNVRLSGLVGELNQEFGPKTMFVYANSYAVFMKIIVNYQQYGFVNAKQP 300

Query: 338 CCGGYFPPFICY-----KDQNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDE 397
           CCG YFPPFICY     +DQN+  SS LCDDRSKYVFWDAYHPTEAANIIIA+ELLDGDE
Sbjct: 301 CCGAYFPPFICYNKGQSQDQNRSSSSFLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDE 360

Query: 398 TISSPINIRQLYAYR 407
           TIS+PINIRQL+AYR
Sbjct: 361 TISAPINIRQLHAYR 373

BLAST of Lag0032557 vs. ExPASy TrEMBL
Match: A0A1S3AUU3 (GDSL esterase/lipase At5g41890 OS=Cucumis melo OX=3656 GN=LOC103483079 PE=3 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 3.6e-177
Identity = 313/373 (83.91%), Postives = 338/373 (90.62%), Query Frame = 0

Query: 38  MEFSKRYTLMFIILSMNFL-RCLCFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKP 97
           M FSK Y +M I+ S+NF    LCFT+FVFGDSLVDAGNNDYIFTLSKA+ PPYGIDFKP
Sbjct: 1   MVFSKSYVVMVIVFSINFFDGGLCFTTFVFGDSLVDAGNNDYIFTLSKANSPPYGIDFKP 60

Query: 98  SGGRPTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDT-IYRGINYASGASGILD 157
           SGG+PTGRFTNGRTISDIIGEYLG AKSFPPPFLAP  T+ DT I++GINYASGASGILD
Sbjct: 61  SGGQPTGRFTNGRTISDIIGEYLG-AKSFPPPFLAPISTQSDTIIHKGINYASGASGILD 120

Query: 158 ETGFLFLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPS 217
           ETG LFLGRI L EQVKNFEESR+ MVK+KGENETME+LKN+IFSLTVGSNDIINYIQPS
Sbjct: 121 ETGLLFLGRISLREQVKNFEESRNEMVKLKGENETMEVLKNSIFSLTVGSNDIINYIQPS 180

Query: 218 IPFFQRNKPAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSE 277
           IPFFQ NKP+PSDYLDHMISNLT+HLKRLH LGARKFVVVG+GPLGCIPFVRA+ FVT+E
Sbjct: 181 IPFFQTNKPSPSDYLDHMISNLTVHLKRLHALGARKFVVVGVGPLGCIPFVRAIHFVTNE 240

Query: 278 RCSEEVNQLIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQ 337
           +C EEVN+LI+ YN +LS VVDQLN EFGL TMFVYANSYAVF KIIVNYRQYGFVNA Q
Sbjct: 241 KCLEEVNRLIETYNFKLSGVVDQLNLEFGLNTMFVYANSYAVFTKIIVNYRQYGFVNAKQ 300

Query: 338 PCCGGYFPPFICYKD-QNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETIS 397
           PCC GYFPPFICYKD QNQ  +S LCDDRSKYVFWDAYHPTEAANIIIA+ELLDGDETI+
Sbjct: 301 PCCVGYFPPFICYKDRQNQSSTSFLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETIT 360

Query: 398 SPINIRQLYAYRL 408
           SPINIRQLYAYRL
Sbjct: 361 SPINIRQLYAYRL 372

BLAST of Lag0032557 vs. ExPASy TrEMBL
Match: A0A6J1GD95 (GDSL esterase/lipase At5g41890 OS=Cucurbita moschata OX=3662 GN=LOC111453124 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 6.3e-174
Identity = 304/368 (82.61%), Postives = 332/368 (90.22%), Query Frame = 0

Query: 44  YTLMFIILSMNFLRCLCFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGRPTG 103
           Y  +F++ S+NF   LCFT+FVFGDSLVDAGNNDYIFTLSKAD PPYGIDFKPSGG+PTG
Sbjct: 8   YITIFVVFSINFFPGLCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKPSGGQPTG 67

Query: 104 RFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDTIYRGINYASGASGILDETGFLFLG 163
           RFTNGRTISDIIGEYLG A+SFPPPFLAPN T+ DTI+ GINYASGA+GILDETGFLFLG
Sbjct: 68  RFTNGRTISDIIGEYLG-AESFPPPFLAPN-TESDTIFTGINYASGAAGILDETGFLFLG 127

Query: 164 RIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPSIPFFQRNK 223
           RIPL EQVK+F+ESRD++VKVKGENETM+LLK AIFSLT GSNDIINY QPSIPFF  NK
Sbjct: 128 RIPLREQVKHFKESRDYIVKVKGENETMKLLKEAIFSLTFGSNDIINYFQPSIPFFGSNK 187

Query: 224 PAPSDYLDHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSEEVNQ 283
            APSDYLD+MISNLTIHLK LHELGARKFVVVG+GPLGCIPFVRAV FV  E+CSEEVN+
Sbjct: 188 VAPSDYLDYMISNLTIHLKELHELGARKFVVVGVGPLGCIPFVRAVQFVRGEKCSEEVNE 247

Query: 284 LIKAYNSRLSKVVDQLNQEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQPCCGGYFP 343
           +I+AYN RLS +V +LNQEFG KTMFVYANSYAVFMKIIVNYRQYGFVNA QPCCG YFP
Sbjct: 248 VIEAYNVRLSGLVGELNQEFGPKTMFVYANSYAVFMKIIVNYRQYGFVNAKQPCCGAYFP 307

Query: 344 PFICY-----KDQNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSPIN 403
           PFICY     +DQN+  SS LCDDRSKYVFWDAYHPTEAANIIIA+ELLDGDETIS+PIN
Sbjct: 308 PFICYNKGQSQDQNRSSSSFLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISTPIN 367

Query: 404 IRQLYAYR 407
           IRQL+AYR
Sbjct: 368 IRQLHAYR 373

BLAST of Lag0032557 vs. TAIR 10
Match: AT5G41890.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 477.2 bits (1227), Expect = 1.3e-134
Identity = 228/366 (62.30%), Postives = 293/366 (80.05%), Query Frame = 0

Query: 42  KRYTLMFIILSM-NFLRCLCFTSFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGR 101
           K +   F++L + +F     FT+F+FGDSLVD GNN+YIFTLSKAD  PYGIDF PS G+
Sbjct: 10  KPFNCTFLLLWLSHFQAAQSFTNFIFGDSLVDVGNNNYIFTLSKADSSPYGIDFAPSNGQ 69

Query: 102 PTGRFTNGRTISDIIGEYLGAAKSFPPPFLAPNITKEDTIYRGINYASGASGILDETGFL 161
           PTGRFTNGRTISDI+GE LG AKS PPP+L PN T+ +TI  GINYASGA+GILD+TG L
Sbjct: 70  PTGRFTNGRTISDIVGEALG-AKSPPPPYLEPN-TEANTIRNGINYASGAAGILDDTGLL 129

Query: 162 FLGRIPLGEQVKNFEESRDFMVKVKGENETMELLKNAIFSLTVGSNDIINYIQPSIPFFQ 221
           F+GR+PL EQV NFE+SR++MV+V GEN T E+LKNA+F++T+GSNDI+NYIQPSIPFF 
Sbjct: 130 FIGRVPLREQVSNFEKSREYMVRVIGENGTKEMLKNAMFTITIGSNDILNYIQPSIPFFS 189

Query: 222 RNKPAPSDYL-DHMISNLTIHLKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSE 281
           ++K  P+D L D M+ +LT HLKRLH+LG RKFVVVG+GPLGCIPF RA+  + + +CSE
Sbjct: 190 QDK-LPTDVLQDSMVLHLTTHLKRLHQLGGRKFVVVGVGPLGCIPFARALNLIPAGKCSE 249

Query: 282 EVNQLIKAYNSRLSKVVDQLNQEF---GLKTMFVYANSYAVFMKIIVNYRQYGFVNANQP 341
           +VNQ+++ YN +L   +  LN E       T FVYANSY +F+K+++NY+ +G  NA++P
Sbjct: 250 QVNQVVRGYNMKLIHSLKTLNNELRSEDYNTTFVYANSYDLFLKLVLNYQLFGLKNADKP 309

Query: 342 CCGGYFPPFICYKDQNQDKSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSP 401
           CCGGYFPPF C+K  NQ+ S + C+DRSK+VFWDAYHPTEAAN+I+A+ LLDGD+T+++P
Sbjct: 310 CCGGYFPPFACFKGPNQNSSQAACEDRSKFVFWDAYHPTEAANLIVAKALLDGDQTVATP 369

Query: 402 INIRQL 403
            NIR L
Sbjct: 370 FNIRYL 372

BLAST of Lag0032557 vs. TAIR 10
Match: AT2G23540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 307.0 bits (785), Expect = 2.3e-83
Identity = 157/345 (45.51%), Postives = 229/345 (66.38%), Query Frame = 0

Query: 63  SFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGRPTGRFTNGRTISDIIGEYLGAA 122
           SF+FGDSLVDAGNN+Y+ TLS+A++ P GIDFK SGG PTGRFTNGRTI DI+GE LG+A
Sbjct: 49  SFIFGDSLVDAGNNNYLSTLSRANMKPNGIDFKASGGTPTGRFTNGRTIGDIVGEELGSA 108

Query: 123 KSFPPPFLAPNITKEDTIYRGINYASGASGILDETGFLFLGRIPLGEQVKNFEESRDFMV 182
            ++  PFLAP+  K   +  G+NYASG  GI++ TG +F+ R+ +  QV  F  +R    
Sbjct: 109 -NYAIPFLAPD-AKGKALLAGVNYASGGGGIMNATGRIFVNRLGMDVQVDFFNTTRKQFD 168

Query: 183 KVKGENETME-LLKNAIFSLTVGSNDII-NYIQPSIPFFQRNKPAPSDYLDHMISNLTIH 242
            + G+ +  + + K +IFS+T+G+ND + NY+ P +    R    P D++  M+ +L   
Sbjct: 169 DLLGKEKAKDYIAKKSIFSITIGANDFLNNYLFPLLSVGTRFTQTPDDFIGDMLEHLRDQ 228

Query: 243 LKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSEEVNQLIKAYNSRLSKVVDQLN 302
           L RL++L ARKFV+  +GP+GCIP+ + +  +    C +  N+L   YN RL  ++++LN
Sbjct: 229 LTRLYQLDARKFVIGNVGPIGCIPYQKTINQLDENECVDLANKLANQYNVRLKSLLEELN 288

Query: 303 QEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQPCC--GGYFPPFI-CYKDQNQDKSS 362
           ++     MFV+AN Y + M++I NY +YGF +A + CC  GG +   I C        +S
Sbjct: 289 KKLP-GAMFVHANVYDLVMELITNYDKYGFKSATKACCGNGGQYAGIIPC------GPTS 348

Query: 363 SLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSPINIRQL 403
           SLC++R KYVFWD YHP+EAAN+IIA++LL GD  + SP+N+ +L
Sbjct: 349 SLCEERDKYVFWDPYHPSEAANVIIAKQLLYGDVKVISPVNLSKL 384

BLAST of Lag0032557 vs. TAIR 10
Match: AT3G50400.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.9e-83
Identity = 161/348 (46.26%), Postives = 227/348 (65.23%), Query Frame = 0

Query: 63  SFVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGRPTGRFTNGRTISDIIGEYLGAA 122
           SFVFGDSLVDAGNN+Y+ TLS+A+ PP GIDFKPS G PTGRFTNGRTI+DI+GE LG  
Sbjct: 35  SFVFGDSLVDAGNNNYLQTLSRANSPPNGIDFKPSRGNPTGRFTNGRTIADIVGEKLG-Q 94

Query: 123 KSFPPPFLAPNITKEDTIYRGINYASGASGILDETGFLFLGRIPLGEQVKNFEESRDFMV 182
           +S+  P+LAPN + E  +  G+NYASG  GIL+ TG +F+ R+ +  QV  F  +R    
Sbjct: 95  QSYAVPYLAPNASGE-ALLNGVNYASGGGGILNATGSVFVNRLGMDIQVDYFTNTRKQFD 154

Query: 183 KVKGENETMELL-KNAIFSLTVGSNDII-NYIQPSIPFFQRNKPAPSDYLDHMISNLTIH 242
           K+ G+++  + + K ++FS+ +GSND + NY+ P +    R    P  ++D MIS+L   
Sbjct: 155 KLLGQDKARDYIRKRSLFSVVIGSNDFLNNYLVPFVAAQARLTQTPETFVDDMISHLRNQ 214

Query: 243 LKRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSEEVNQLIKAYNSRLSKVVDQLN 302
           LKRL+++ ARKFVV  + P+GCIP+ +++  +  ++C +  N+L   YN+RL  ++    
Sbjct: 215 LKRLYDMDARKFVVGNVAPIGCIPYQKSINQLNDKQCVDLANKLAIQYNARLKDLLTVEL 274

Query: 303 QEFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQPCC------GGYFPPFICYKDQNQD 362
           ++      FVYAN Y +FM +IVN++ YGF  A++ CC       G  P   C       
Sbjct: 275 KDSLKDAHFVYANVYDLFMDLIVNFKDYGFRTASEACCETRGRLAGILP---C------G 334

Query: 363 KSSSLCDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSPINIRQL 403
            +SSLC DRSK+VFWDAYHPTEAAN++IA +LL GD    +P N+  L
Sbjct: 335 PTSSLCTDRSKHVFWDAYHPTEAANLLIADKLLYGDSKFVTPFNLLHL 371

BLAST of Lag0032557 vs. TAIR 10
Match: AT1G71691.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.5e-63
Identity = 138/346 (39.88%), Postives = 200/346 (57.80%), Query Frame = 0

Query: 64  FVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGRPTGRFTNGRTISDIIGEYLGAAK 123
           FVFGDSL+D GNN+ I + +KA+  PYGIDF    G PTGRF NG T+ D I + LG   
Sbjct: 57  FVFGDSLIDNGNNNNIPSFAKANYFPYGIDF---NGGPTGRFCNGLTMVDGIAQLLGL-- 116

Query: 124 SFPPPFLAPNITKEDTIYRGINYASGASGILDETGFLFLGRIPLGEQVKNFEESRDFMV- 183
              P   A +    D + RG+NYAS A+GIL +TG  F+GRIP  +Q+ NFE + D +  
Sbjct: 117 ---PLIPAYSEATGDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFETTLDQVAS 176

Query: 184 KVKGENETMELLKNAIFSLTVGSNDII-NYIQPSIPFFQRNKPAPSDYLDHMISNLTIHL 243
           K  G     + +  ++F + +GSND + NY+ P+ P   RN+     + D ++ + T  L
Sbjct: 177 KSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFP--TRNQYNSQQFGDLLVQHYTDQL 236

Query: 244 KRLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSEEVNQLIKAYNSRLSKVVDQLNQ 303
            RL+ LG RKFVV G+G +GCIP + A       +CSEEVNQL+  +N+ +  ++  LNQ
Sbjct: 237 TRLYNLGGRKFVVAGLGRMGCIPSILAQ--GNDGKCSEEVNQLVLPFNTNVKTMISNLNQ 296

Query: 304 EFGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQPCCGGYFPPFICYKDQNQDKSSSL-- 363
                  F+Y +   +F  I+ N   YG    ++ CCG           +N+ + + L  
Sbjct: 297 NLP-DAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCG---------IGKNRGQITCLPF 356

Query: 364 ---CDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSPINIRQL 403
              C +R +YVFWDA+HPTE  N+I+A++   GD T++ PINI+QL
Sbjct: 357 ETPCPNRDQYVFWDAFHPTEKVNLIMAKKAFAGDRTVAYPINIQQL 380

BLAST of Lag0032557 vs. TAIR 10
Match: AT5G08460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.5e-63
Identity = 134/347 (38.62%), Postives = 203/347 (58.50%), Query Frame = 0

Query: 64  FVFGDSLVDAGNNDYIFTLSKADLPPYGIDFKPSGGRPTGRFTNGRTISDIIGEYLGAAK 123
           FVFGDSLVD GNN+++ +L++++  PYGIDF  +G +PTGRF+NG+TI D IGE LG  +
Sbjct: 51  FVFGDSLVDNGNNNHLNSLARSNYLPYGIDF--AGNQPTGRFSNGKTIVDFIGELLGLPE 110

Query: 124 SFPPPFLAPNITKEDTIYRGINYASGASGILDETGFLFLGRIPLGEQVKNFEESRDFMVK 183
               P     +     I  G+NYAS A GIL+ETG     R  +G QV+NFE++   + +
Sbjct: 111 I---PAFMDTVDGGVDILHGVNYASAAGGILEETGRHLGERFSMGRQVENFEKTLMEISR 170

Query: 184 VKGENETMELLKNAIFSLTVGSNDII-NYIQPSIPFFQRNKPAPSDYLDHMISNLTIHLK 243
              +    E +  ++  +++G+ND I NY++P + F   +   P+ + D ++SN T HL 
Sbjct: 171 SMRKESVKEYMAKSLVVVSLGNNDYINNYLKPRL-FLSSSIYDPTSFADLLLSNFTTHLL 230

Query: 244 RLHELGARKFVVVGIGPLGCIPFVRAVFFVTSERCSEEVNQLIKAYNSRLSKVVDQLNQE 303
            L+  G RKFV+ G+GPLGCIP   A        C E VN++ + +N+RL  +VD+LN +
Sbjct: 231 ELYGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAELFNNRLVSLVDRLNSD 290

Query: 304 --FGLKTMFVYANSYAVFMKIIVNYRQYGFVNANQPCCGGYFPPFICYKDQNQDKSSSL- 363
                + +FVY N+Y   + I+ N   YGF   ++ CCG           +N+ + + L 
Sbjct: 291 NKTASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCG---------VGRNRGEITCLP 350

Query: 364 ----CDDRSKYVFWDAYHPTEAANIIIAQELLDGDETISSPINIRQL 403
               C  R ++VFWDA+HPT+A N+IIA    +G ++   PIN+ QL
Sbjct: 351 LAVPCAFRDRHVFWDAFHPTQAFNLIIALRAFNGSKSDCYPINLSQL 382

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004143942.37.9e-17984.14GDSL esterase/lipase At5g41890 [Cucumis sativus][more]
XP_022158401.18.7e-17883.29GDSL esterase/lipase At5g41890 [Momordica charantia][more]
XP_022977773.12.5e-17783.20GDSL esterase/lipase At5g41890 [Cucurbita maxima][more]
XP_008437733.17.4e-17783.91PREDICTED: GDSL esterase/lipase At5g41890 [Cucumis melo][more]
XP_038907274.14.8e-17683.78GDSL esterase/lipase At5g41890 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FJ251.8e-13364.74GDSL esterase/lipase At5g41890 OS=Arabidopsis thaliana OX=3702 GN=At5g41890 PE=3... [more]
O804703.2e-8245.51GDSL esterase/lipase At2g23540 OS=Arabidopsis thaliana OX=3702 GN=At2g23540 PE=2... [more]
Q9M2R95.5e-8246.26GDSL esterase/lipase At3g50400 OS=Arabidopsis thaliana OX=3702 GN=At3g50400 PE=2... [more]
O234705.5e-8247.58GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3... [more]
Q9SF782.2e-6239.88GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0KM973.8e-17984.14Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148500 PE=3 SV=1[more]
A0A6J1E0T34.2e-17883.29GDSL esterase/lipase At5g41890 OS=Momordica charantia OX=3673 GN=LOC111024890 PE... [more]
A0A6J1IR231.2e-17783.20GDSL esterase/lipase At5g41890 OS=Cucurbita maxima OX=3661 GN=LOC111477974 PE=3 ... [more]
A0A1S3AUU33.6e-17783.91GDSL esterase/lipase At5g41890 OS=Cucumis melo OX=3656 GN=LOC103483079 PE=3 SV=1[more]
A0A6J1GD956.3e-17482.61GDSL esterase/lipase At5g41890 OS=Cucurbita moschata OX=3662 GN=LOC111453124 PE=... [more]
Match NameE-valueIdentityDescription
AT5G41890.11.3e-13462.30GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT2G23540.12.3e-8345.51GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G50400.13.9e-8346.26GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G71691.21.5e-6339.88GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G08460.11.5e-6338.62GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 62..391
e-value: 1.1E-76
score: 260.3
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 64..386
e-value: 5.4E-28
score: 99.0
NoneNo IPR availablePANTHERPTHR45648:SF25GDSL-LIKE LIPASE/ACYLHYDROLASE SUPERFAMILY PROTEINcoord: 45..404
NoneNo IPR availablePANTHERPTHR45648GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)coord: 45..404
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 194..388
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 64..390
e-value: 2.45743E-137
score: 393.519

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032557.1Lag0032557.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds