Lag0032509 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032509
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein DMR6-LIKE OXYGENASE 2-like
Locationchr11: 33736981 .. 33737774 (+)
RNA-Seq ExpressionLag0032509
SyntenyLag0032509
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTTATCGCTCACTTGTAGAAAAGTAGTGGCGGAGTACTGTACTAGAGTGAGACAGTTGACATTGGAGCTACTAGAGGCCATTTCTGAGAGCTTGGGTTTGTCAAAAGGTTCCATAGCCAACTCAATTGGGAGCCACGGCCAACACATGGCCTTCAACTACTACCCGCCATGTCCACAGCCAGAGCTCACCTATGGCTTGTCCGGCCACACTGACCCAAACCTCATCACTCTCCTCCTCCAAGACCAAGTCCCTGGCCTCCAAGTCCTCCGCGACGGTGCCTGGGTCTCACTCAACCCCATCCCCAACACTTTCATCATCAACATCGGTGACCAGATGCAGGTATATATAACACTATCCATATATTACTTTATCTTCACCATAAATATTACAAAATTATATTTGCATGGATACACGAAAAGATAGAGACTGAATAAGGTCGGTCGACGTCAGTTTTGGGCTCAAACTCAGTTGGAGATTTATGCTCCGAGCGCCAGAGATTTCTGAAAGTGGGTTTTCGTGTTTGATTTTTTTGTTCTCAGGCATTGAGTAACGACAGGTACAAAAGCGTGCTTCATCGGGCTGTTGTGAATAATGCAAAAGAGCGAATTTCGATACCGACGTTTTACTGTCCGTCGCCGGAGGCCATGATCGGCCCAGTCAAGGAGTTGCTCGACGATGAACATGGAGCTGTGTATAGAAACTTTTCTTATTCTGAATATTACCAGACCTTTTGGAGTCAAGGACTTGCGACTGAAAGATGCTTAGATTTTTTTAGAGCTACTGAATGA

mRNA sequence

ATGGTTTTATCGCTCACTTGTAGAAAAGTAGTGGCGGAGTACTGTACTAGAGTGAGACAGTTGACATTGGAGCTACTAGAGGCCATTTCTGAGAGCTTGGGTTTGTCAAAAGGTTCCATAGCCAACTCAATTGGGAGCCACGGCCAACACATGGCCTTCAACTACTACCCGCCATGTCCACAGCCAGAGCTCACCTATGGCTTGTCCGGCCACACTGACCCAAACCTCATCACTCTCCTCCTCCAAGACCAAGTCCCTGGCCTCCAAGTCCTCCGCGACGGTGCCTGGGTCTCACTCAACCCCATCCCCAACACTTTCATCATCAACATCGGTGACCAGATGCAGGCATTGAGTAACGACAGGTACAAAAGCGTGCTTCATCGGGCTGTTGTGAATAATGCAAAAGAGCGAATTTCGATACCGACGTTTTACTGTCCGTCGCCGGAGGCCATGATCGGCCCAGTCAAGGAGTTGCTCGACGATGAACATGGAGCTGTGTATAGAAACTTTTCTTATTCTGAATATTACCAGACCTTTTGGAGTCAAGGACTTGCGACTGAAAGATGCTTAGATTTTTTTAGAGCTACTGAATGA

Coding sequence (CDS)

ATGGTTTTATCGCTCACTTGTAGAAAAGTAGTGGCGGAGTACTGTACTAGAGTGAGACAGTTGACATTGGAGCTACTAGAGGCCATTTCTGAGAGCTTGGGTTTGTCAAAAGGTTCCATAGCCAACTCAATTGGGAGCCACGGCCAACACATGGCCTTCAACTACTACCCGCCATGTCCACAGCCAGAGCTCACCTATGGCTTGTCCGGCCACACTGACCCAAACCTCATCACTCTCCTCCTCCAAGACCAAGTCCCTGGCCTCCAAGTCCTCCGCGACGGTGCCTGGGTCTCACTCAACCCCATCCCCAACACTTTCATCATCAACATCGGTGACCAGATGCAGGCATTGAGTAACGACAGGTACAAAAGCGTGCTTCATCGGGCTGTTGTGAATAATGCAAAAGAGCGAATTTCGATACCGACGTTTTACTGTCCGTCGCCGGAGGCCATGATCGGCCCAGTCAAGGAGTTGCTCGACGATGAACATGGAGCTGTGTATAGAAACTTTTCTTATTCTGAATATTACCAGACCTTTTGGAGTCAAGGACTTGCGACTGAAAGATGCTTAGATTTTTTTAGAGCTACTGAATGA

Protein sequence

MVLSLTCRKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYGLSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLHRAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLATERCLDFFRATE
Homology
BLAST of Lag0032509 vs. NCBI nr
Match: XP_022159616.1 (protein DMR6-LIKE OXYGENASE 2-like [Momordica charantia])

HSP 1 Score: 352.1 bits (902), Expect = 3.2e-93
Identity = 166/189 (87.83%), Postives = 177/189 (93.65%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           RK VAEYCT VR+LTL LLEAISESLGL K SI NSIGSHGQHMA NYYPPCPQPELTYG
Sbjct: 161 RKYVAEYCTSVRELTLNLLEAISESLGLPKDSITNSIGSHGQHMALNYYPPCPQPELTYG 220

Query: 68  LSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLH 127
           L GHTDPNLITLLLQDQVPGLQVLRDGAWV+LNPIPNTFIINIGDQMQALSND+YKSVLH
Sbjct: 221 LPGHTDPNLITLLLQDQVPGLQVLRDGAWVALNPIPNTFIINIGDQMQALSNDKYKSVLH 280

Query: 128 RAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLATE 187
           RAVVN+ KERISIPTFYCPSPEA+IGP+KELL++EH AVYRNFSYSEYYQTFW++GLATE
Sbjct: 281 RAVVNSTKERISIPTFYCPSPEAVIGPIKELLNNEHEAVYRNFSYSEYYQTFWNEGLATE 340

Query: 188 RCLDFFRAT 197
           RCLD F+ T
Sbjct: 341 RCLDMFKVT 349

BLAST of Lag0032509 vs. NCBI nr
Match: XP_004145878.1 (protein DMR6-LIKE OXYGENASE 2 [Cucumis sativus] >KGN49964.1 hypothetical protein Csa_000039 [Cucumis sativus])

HSP 1 Score: 331.6 bits (849), Expect = 4.5e-87
Identity = 159/187 (85.03%), Postives = 167/187 (89.30%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           RK VAEYCT +RQLTL+LLEAISESLGL K SIANSIGSHGQHMA NYYPPCPQP+LTYG
Sbjct: 160 RKEVAEYCTTMRQLTLKLLEAISESLGLPKDSIANSIGSHGQHMALNYYPPCPQPDLTYG 219

Query: 68  LSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLH 127
           L  HTDPNLITLLLQDQVPGLQV RDGAWV+LNPIPNTFIINIGDQMQ LSNDRYKSVLH
Sbjct: 220 LPCHTDPNLITLLLQDQVPGLQVHRDGAWVALNPIPNTFIINIGDQMQVLSNDRYKSVLH 279

Query: 128 RAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLATE 187
           RAVVNN+ ERISIPTFYCPSPEAMIGP KEL+ DEH   +RNF+YSEYYQTFWS  L T 
Sbjct: 280 RAVVNNSTERISIPTFYCPSPEAMIGPAKELIHDEHRPAFRNFTYSEYYQTFWSGELDTR 339

Query: 188 RCLDFFR 195
           RCLD FR
Sbjct: 340 RCLDLFR 346

BLAST of Lag0032509 vs. NCBI nr
Match: XP_038876327.1 (protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida])

HSP 1 Score: 330.5 bits (846), Expect = 1.0e-86
Identity = 157/187 (83.96%), Postives = 167/187 (89.30%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           RK VAEYCT +RQLTL+LLEAISESLGL K SIANSIGSHGQHMA NYYPPCPQPELTYG
Sbjct: 160 RKEVAEYCTSMRQLTLKLLEAISESLGLPKDSIANSIGSHGQHMALNYYPPCPQPELTYG 219

Query: 68  LSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLH 127
           L  HTDPNLITLLLQDQ+PGLQV  DGAWV+LNPIP+TFI+NIGDQMQ LSNDRYKSVLH
Sbjct: 220 LPCHTDPNLITLLLQDQIPGLQVYHDGAWVALNPIPDTFIVNIGDQMQVLSNDRYKSVLH 279

Query: 128 RAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLATE 187
           RAVVNN  ERISIPTFYCPSPEAMIGP KEL+D EH AVYR+F+YSEYY+TFWS  L T 
Sbjct: 280 RAVVNNVTERISIPTFYCPSPEAMIGPAKELIDSEHRAVYRHFTYSEYYKTFWSGELDTR 339

Query: 188 RCLDFFR 195
           RCLD FR
Sbjct: 340 RCLDLFR 346

BLAST of Lag0032509 vs. NCBI nr
Match: KAG6579599.1 (Protein DMR6-LIKE OXYGENASE 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 325.9 bits (834), Expect = 2.5e-85
Identity = 154/187 (82.35%), Postives = 168/187 (89.84%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           RK VAEYCT VR+LTL+LLEAISESLGL K SIANSIG HGQHMA NYYPPCPQPELTYG
Sbjct: 158 RKEVAEYCTSVRELTLKLLEAISESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTYG 217

Query: 68  LSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLH 127
           L GHTDPNLITLLLQDQVPGLQ L D  W +L+PIPNTFIINIGDQMQALSNDRYKSVLH
Sbjct: 218 LPGHTDPNLITLLLQDQVPGLQALHDNTWFALDPIPNTFIINIGDQMQALSNDRYKSVLH 277

Query: 128 RAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLATE 187
           RAVVN+  ERISIPTFYCPS +AMIGP KEL+DD++ AVYR F+YSEYY+TFW++GLAT+
Sbjct: 278 RAVVNSETERISIPTFYCPSQDAMIGPFKELVDDKNRAVYRQFTYSEYYKTFWNEGLATK 337

Query: 188 RCLDFFR 195
           RCLD FR
Sbjct: 338 RCLDLFR 344

BLAST of Lag0032509 vs. NCBI nr
Match: XP_022929059.1 (protein DMR6-LIKE OXYGENASE 2-like [Cucurbita moschata])

HSP 1 Score: 325.9 bits (834), Expect = 2.5e-85
Identity = 154/187 (82.35%), Postives = 168/187 (89.84%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           RK VAEYCT VR+LTL+LLEAISESLGL K SIANSIG HGQHMA NYYPPCPQPELTYG
Sbjct: 158 RKEVAEYCTSVRELTLKLLEAISESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTYG 217

Query: 68  LSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLH 127
           L GHTDPNLITLLLQDQVPGLQ L D  W +L+PIPNTFIINIGDQMQALSNDRYKSVLH
Sbjct: 218 LPGHTDPNLITLLLQDQVPGLQALHDNTWFALDPIPNTFIINIGDQMQALSNDRYKSVLH 277

Query: 128 RAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLATE 187
           RAVVN+  ERISIPTFYCPS +AMIGP KEL+DD++ AVYR F+YSEYY+TFW++GLAT+
Sbjct: 278 RAVVNSETERISIPTFYCPSQDAMIGPFKELVDDKNRAVYRQFTYSEYYKTFWNEGLATK 337

Query: 188 RCLDFFR 195
           RCLD FR
Sbjct: 338 RCLDLFR 344

BLAST of Lag0032509 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.1e-72
Identity = 124/194 (63.92%), Postives = 158/194 (81.44%), Query Frame = 0

Query: 5   LTCRKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPEL 64
           ++ R+V AEY T VR L L LLEAISESLGL+K  ++N+IG HGQHMA NYYP CPQPEL
Sbjct: 153 ISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPEL 212

Query: 65  TYGLSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKS 124
           TYGL GH D NLIT+LLQD+V GLQV +DG W+++NP+PNTFI+N+GDQMQ +SN++YKS
Sbjct: 213 TYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKS 272

Query: 125 VLHRAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHG--AVYRNFSYSEYYQTFWSQ 184
           VLHRAVVN+  ERISIPTFYCPS +A+I P +EL+++E    A+YRNF+Y+EY++ FW  
Sbjct: 273 VLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEKFWDT 332

Query: 185 GLATERCLDFFRAT 197
              TE C+D F+A+
Sbjct: 333 AFDTESCIDSFKAS 346

BLAST of Lag0032509 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 2.6e-69
Identity = 122/192 (63.54%), Postives = 151/192 (78.65%), Query Frame = 0

Query: 5   LTCRKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPEL 64
           ++ R+V AEY T VR L L LLEAISESLGL    I+N +G H QHMAFNYYPPCP+PEL
Sbjct: 155 ISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNILGKHAQHMAFNYYPPCPEPEL 214

Query: 65  TYGLSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKS 124
           TYGL GH DP +IT+LLQDQV GLQV +D  WV+++PIPNTFI+NIGDQMQ +SND+YKS
Sbjct: 215 TYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKS 274

Query: 125 VLHRAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHG-AVYRNFSYSEYYQTFWSQG 184
           VLHRAVVN   ER+SIPTFY PS +A+IGP  EL++++   A+YR + + EY+  FW++ 
Sbjct: 275 VLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSLAIYRTYPFVEYWDKFWNRS 334

Query: 185 LATERCLDFFRA 196
           LAT  CLD F+A
Sbjct: 335 LATASCLDAFKA 346

BLAST of Lag0032509 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 4.4e-61
Identity = 106/188 (56.38%), Postives = 138/188 (73.40%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           ++++  YCT VR+L   L EAISESLGL  G +  ++G   QHMA NYYP CP+PELTYG
Sbjct: 154 KEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQHMAVNYYPQCPEPELTYG 213

Query: 68  LSGHTDPNLIT-LLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVL 127
           L  HTDPN +T LL+ DQV GLQVL DG W+++NP P   +INIGDQ+QALSN +Y+SV 
Sbjct: 214 LPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVINIGDQLQALSNGKYRSVW 273

Query: 128 HRAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLAT 187
           HRAVVN+ +ER+S+ +F CP     +GP K+L+ D+  AVYRN++Y EYY+ FWS+ L  
Sbjct: 274 HRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRNYTYDEYYKKFWSRNLDQ 333

Query: 188 ERCLDFFR 195
           E CL+ FR
Sbjct: 334 EHCLELFR 341

BLAST of Lag0032509 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.4e-54
Identity = 102/190 (53.68%), Postives = 135/190 (71.05%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           +++V++Y   VR++  ++ E ISESLGL K  +   +G  GQHMA NYYPPCP+PELTYG
Sbjct: 149 KEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYG 208

Query: 68  LSGHTDPNLITLLLQD-QVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVL 127
           L  HTDPN +T+LLQD  V GLQ+L DG W ++NP P+ F+INIGDQ+QALSN  YKSV 
Sbjct: 209 LPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINIGDQLQALSNGVYKSVW 268

Query: 128 HRAVVNNAKERISIPTFYCPSPEAMIGPVK---ELLDDEHGAVYRNFSYSEYYQTFWSQG 187
           HRAV N    R+S+ +F CP+  A++ P K   E  DDE   VY++F+Y+EYY+ FWS+ 
Sbjct: 269 HRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVYKDFTYAEYYKKFWSRN 328

Query: 188 LATERCLDFF 194
           L  E CL+ F
Sbjct: 329 LDQEHCLENF 338

BLAST of Lag0032509 vs. ExPASy Swiss-Prot
Match: Q6YYX9 (Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC1 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.5e-37
Identity = 67/160 (41.88%), Postives = 108/160 (67.50%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           R+V + Y    +++ +E++EA  E+LG+  G +   + +  Q M  N YP CPQPELT G
Sbjct: 195 REVASRYAEANQRVFMEVMEAALEALGVGGGGVMEDLAAGTQMMTVNCYPECPQPELTLG 254

Query: 68  LSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLH 127
           +  H+D   +TL+LQD+V GLQV+  G W++++P+P +F++N+GD ++ LSN RY+SVLH
Sbjct: 255 MPPHSDYGFLTLVLQDEVAGLQVMHAGEWLTVDPLPGSFVVNVGDHLEILSNGRYRSVLH 314

Query: 128 RAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVY 168
           R  VN+ + R+S+ +F+  +PE ++ P  EL+DD H   Y
Sbjct: 315 RVKVNSRRLRVSVASFHSVAPERVVSPAPELIDDRHPRRY 354

BLAST of Lag0032509 vs. ExPASy TrEMBL
Match: A0A6J1E4G7 (protein DMR6-LIKE OXYGENASE 2-like OS=Momordica charantia OX=3673 GN=LOC111025975 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.6e-93
Identity = 166/189 (87.83%), Postives = 177/189 (93.65%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           RK VAEYCT VR+LTL LLEAISESLGL K SI NSIGSHGQHMA NYYPPCPQPELTYG
Sbjct: 161 RKYVAEYCTSVRELTLNLLEAISESLGLPKDSITNSIGSHGQHMALNYYPPCPQPELTYG 220

Query: 68  LSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLH 127
           L GHTDPNLITLLLQDQVPGLQVLRDGAWV+LNPIPNTFIINIGDQMQALSND+YKSVLH
Sbjct: 221 LPGHTDPNLITLLLQDQVPGLQVLRDGAWVALNPIPNTFIINIGDQMQALSNDKYKSVLH 280

Query: 128 RAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLATE 187
           RAVVN+ KERISIPTFYCPSPEA+IGP+KELL++EH AVYRNFSYSEYYQTFW++GLATE
Sbjct: 281 RAVVNSTKERISIPTFYCPSPEAVIGPIKELLNNEHEAVYRNFSYSEYYQTFWNEGLATE 340

Query: 188 RCLDFFRAT 197
           RCLD F+ T
Sbjct: 341 RCLDMFKVT 349

BLAST of Lag0032509 vs. ExPASy TrEMBL
Match: A0A0A0KM81 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G146870 PE=3 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.2e-87
Identity = 159/187 (85.03%), Postives = 167/187 (89.30%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           RK VAEYCT +RQLTL+LLEAISESLGL K SIANSIGSHGQHMA NYYPPCPQP+LTYG
Sbjct: 160 RKEVAEYCTTMRQLTLKLLEAISESLGLPKDSIANSIGSHGQHMALNYYPPCPQPDLTYG 219

Query: 68  LSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLH 127
           L  HTDPNLITLLLQDQVPGLQV RDGAWV+LNPIPNTFIINIGDQMQ LSNDRYKSVLH
Sbjct: 220 LPCHTDPNLITLLLQDQVPGLQVHRDGAWVALNPIPNTFIINIGDQMQVLSNDRYKSVLH 279

Query: 128 RAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLATE 187
           RAVVNN+ ERISIPTFYCPSPEAMIGP KEL+ DEH   +RNF+YSEYYQTFWS  L T 
Sbjct: 280 RAVVNNSTERISIPTFYCPSPEAMIGPAKELIHDEHRPAFRNFTYSEYYQTFWSGELDTR 339

Query: 188 RCLDFFR 195
           RCLD FR
Sbjct: 340 RCLDLFR 346

BLAST of Lag0032509 vs. ExPASy TrEMBL
Match: A0A6J1EMN2 (protein DMR6-LIKE OXYGENASE 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435762 PE=3 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.2e-85
Identity = 154/187 (82.35%), Postives = 168/187 (89.84%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           RK VAEYCT VR+LTL+LLEAISESLGL K SIANSIG HGQHMA NYYPPCPQPELTYG
Sbjct: 158 RKEVAEYCTSVRELTLKLLEAISESLGLPKDSIANSIGGHGQHMALNYYPPCPQPELTYG 217

Query: 68  LSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLH 127
           L GHTDPNLITLLLQDQVPGLQ L D  W +L+PIPNTFIINIGDQMQALSNDRYKSVLH
Sbjct: 218 LPGHTDPNLITLLLQDQVPGLQALHDNTWFALDPIPNTFIINIGDQMQALSNDRYKSVLH 277

Query: 128 RAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLATE 187
           RAVVN+  ERISIPTFYCPS +AMIGP KEL+DD++ AVYR F+YSEYY+TFW++GLAT+
Sbjct: 278 RAVVNSETERISIPTFYCPSQDAMIGPFKELVDDKNRAVYRQFTYSEYYKTFWNEGLATK 337

Query: 188 RCLDFFR 195
           RCLD FR
Sbjct: 338 RCLDLFR 344

BLAST of Lag0032509 vs. ExPASy TrEMBL
Match: A0A5D3C3T0 (Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001360 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.6e-85
Identity = 157/187 (83.96%), Postives = 165/187 (88.24%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           RK VAEYCT +RQLTL+LLEAISESLGL K SIANSIGSHGQHMA NYYPPCPQP+LTYG
Sbjct: 160 RKEVAEYCTSMRQLTLKLLEAISESLGLPKDSIANSIGSHGQHMALNYYPPCPQPDLTYG 219

Query: 68  LSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLH 127
           L  HTDPNLITLLLQDQVPGLQV RDGAWV+L+PIPNTFIINIGDQMQ LSNDRYKSVLH
Sbjct: 220 LPCHTDPNLITLLLQDQVPGLQVHRDGAWVALDPIPNTFIINIGDQMQVLSNDRYKSVLH 279

Query: 128 RAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLATE 187
           RAVVNNA ERISIPTFYCPSPEAMI P KEL+D EH   +R F+YSEYYQTFWS  L T 
Sbjct: 280 RAVVNNATERISIPTFYCPSPEAMIRPAKELIDGEHRPAFRQFTYSEYYQTFWSGELDTR 339

Query: 188 RCLDFFR 195
           RCLD FR
Sbjct: 340 RCLDLFR 346

BLAST of Lag0032509 vs. ExPASy TrEMBL
Match: A0A1S3AUN9 (protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo OX=3656 GN=LOC103482877 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.6e-85
Identity = 157/190 (82.63%), Postives = 165/190 (86.84%), Query Frame = 0

Query: 8   RKVVAEYCTRVRQLTLELLEAISESLGLSKGSIANSIGSHGQHMAFNYYPPCPQPELTYG 67
           RK VAEYCT +RQLTL+LLEAISESLGL K SIANSIGSHGQHMA NYYPPCPQP+LTYG
Sbjct: 160 RKEVAEYCTSMRQLTLKLLEAISESLGLPKDSIANSIGSHGQHMALNYYPPCPQPDLTYG 219

Query: 68  LSGHTDPNLITLLLQDQVPGLQVLRDGAWVSLNPIPNTFIINIGDQMQALSNDRYKSVLH 127
           L  HTDPNLITLLLQDQVPGLQV RDGAWV+L+PIPNTFIINIGDQMQ LSNDRYKSVLH
Sbjct: 220 LPCHTDPNLITLLLQDQVPGLQVHRDGAWVALDPIPNTFIINIGDQMQVLSNDRYKSVLH 279

Query: 128 RAVVNNAKERISIPTFYCPSPEAMIGPVKELLDDEHGAVYRNFSYSEYYQTFWSQGLATE 187
           RAVVNNA ERISIPTFYCPSPEA I P KEL+D EH   +R F+YSEYYQTFWS  L T 
Sbjct: 280 RAVVNNATERISIPTFYCPSPEATIRPAKELIDGEHRPAFRQFTYSEYYQTFWSGELDTR 339

Query: 188 RCLDFFRATE 198
           RCLD FR  E
Sbjct: 340 RCLDLFRVNE 349

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159616.13.2e-9387.83protein DMR6-LIKE OXYGENASE 2-like [Momordica charantia][more]
XP_004145878.14.5e-8785.03protein DMR6-LIKE OXYGENASE 2 [Cucumis sativus] >KGN49964.1 hypothetical protein... [more]
XP_038876327.11.0e-8683.96protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida][more]
KAG6579599.12.5e-8582.35Protein DMR6-LIKE OXYGENASE 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022929059.12.5e-8582.35protein DMR6-LIKE OXYGENASE 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9ZSA71.1e-7263.92Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1[more]
Q9ZSA82.6e-6963.54Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1[more]
Q8W2X54.4e-6156.38Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1... [more]
Q9FLV01.4e-5453.68Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... [more]
Q6YYX91.5e-3741.88Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japoni... [more]
Match NameE-valueIdentityDescription
A0A6J1E4G71.6e-9387.83protein DMR6-LIKE OXYGENASE 2-like OS=Momordica charantia OX=3673 GN=LOC11102597... [more]
A0A0A0KM812.2e-8785.03Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A6J1EMN21.2e-8582.35protein DMR6-LIKE OXYGENASE 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435762... [more]
A0A5D3C3T01.6e-8583.96Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3AUN91.6e-8582.63protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo OX=3656 GN=LOC103482877 PE=3 ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 71..92
score: 48.76
coord: 106..132
score: 31.65
NoneNo IPR availablePANTHERPTHR47991:SF2PROTEIN DMR6-LIKE OXYGENASE 2-LIKEcoord: 7..188
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 7..188
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 7..183
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 49..146
e-value: 4.3E-30
score: 104.2
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 4..183
e-value: 4.7E-67
score: 228.6
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 47..146
score: 14.997054

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032509.1Lag0032509.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1901576 organic substance biosynthetic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen