Lag0032476 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032476
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr11: 33035507 .. 33036007 (+)
RNA-Seq ExpressionLag0032476
SyntenyLag0032476
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGCCAGAGGGTAGAATCGACAACAAGGATCGATGCCTCCGCTTTTGTAACTATCAACAAGATGATTTGAATCTCTTATGTGTGGGAGCATGGACTATCTGGAATGATAGGAACAAAATTGTTCATAATCTACCAGTACCCACTATTGAAATTAAGTGTGACTGGATTTTTGCTTATCTGGAGGAGTTTCAAAAGGTTCAAGTGGCAAGCGGGGCCACTTCTTCACTGTATGCTGAATTTCAGACGCTGATTGCTAGACGGGAGGAGACCATTATGAATACTGATGCGGCTTATGACGCGATTAATCGAAAGATTGGCATTGGGATAGTGATCCGGGATAAGCAGGGCAATCTCAAGGCGGCCCACTCAAAATCTTCTCAGGTTTTTACCACTCCGCTTTGTGCAGAAGCAGCTGCAGTCCTTGAAAGACTTCAGTTTGCTAGAGACATGGATCTGAAATGTATTACGGTCTTGACAGATTCCCTCTTGCTAGAATAG

mRNA sequence

ATGCTGCCAGAGGGTAGAATCGACAACAAGGATCGATGCCTCCGCTTTTGTAACTATCAACAAGATGATTTGAATCTCTTATGTGTGGGAGCATGGACTATCTGGAATGATAGGAACAAAATTGTTCATAATCTACCAGTACCCACTATTGAAATTAAGTGTGACTGGATTTTTGCTTATCTGGAGGAGTTTCAAAAGGTTCAAGTGGCAAGCGGGGCCACTTCTTCACTGTATGCTGAATTTCAGACGCTGATTGCTAGACGGGAGGAGACCATTATGAATACTGATGCGGCTTATGACGCGATTAATCGAAAGATTGGCATTGGGATAGTGATCCGGGATAAGCAGGGCAATCTCAAGGCGGCCCACTCAAAATCTTCTCAGGTTTTTACCACTCCGCTTTGTGCAGAAGCAGCTGCAGTCCTTGAAAGACTTCAGTTTGCTAGAGACATGGATCTGAAATGTATTACGGTCTTGACAGATTCCCTCTTGCTAGAATAG

Coding sequence (CDS)

ATGCTGCCAGAGGGTAGAATCGACAACAAGGATCGATGCCTCCGCTTTTGTAACTATCAACAAGATGATTTGAATCTCTTATGTGTGGGAGCATGGACTATCTGGAATGATAGGAACAAAATTGTTCATAATCTACCAGTACCCACTATTGAAATTAAGTGTGACTGGATTTTTGCTTATCTGGAGGAGTTTCAAAAGGTTCAAGTGGCAAGCGGGGCCACTTCTTCACTGTATGCTGAATTTCAGACGCTGATTGCTAGACGGGAGGAGACCATTATGAATACTGATGCGGCTTATGACGCGATTAATCGAAAGATTGGCATTGGGATAGTGATCCGGGATAAGCAGGGCAATCTCAAGGCGGCCCACTCAAAATCTTCTCAGGTTTTTACCACTCCGCTTTGTGCAGAAGCAGCTGCAGTCCTTGAAAGACTTCAGTTTGCTAGAGACATGGATCTGAAATGTATTACGGTCTTGACAGATTCCCTCTTGCTAGAATAG

Protein sequence

MLPEGRIDNKDRCLRFCNYQQDDLNLLCVGAWTIWNDRNKIVHNLPVPTIEIKCDWIFAYLEEFQKVQVASGATSSLYAEFQTLIARREETIMNTDAAYDAINRKIGIGIVIRDKQGNLKAAHSKSSQVFTTPLCAEAAAVLERLQFARDMDLKCITVLTDSLLLE
Homology
BLAST of Lag0032476 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 75.5 bits (184), Expect = 4.9e-10
Identity = 48/147 (32.65%), Postives = 74/147 (50.34%), Query Frame = 0

Query: 23   DLNLLCVGAWTIWNDRNKIVHNLPVPTIEIKCDWIFAYLEEFQKVQVASGATSSLYAEFQ 82
            DLNL  +  W IWNDRN ++H   V  +E KC+W+  +L+   + Q+ S  +    +  +
Sbjct: 1230 DLNLAAITGWGIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQM-SNYSPRTQSNHR 1289

Query: 83   TLIA--RREETI---MNTDAAYDAINRKIGIGIVIRDKQGNLKAAHSKSSQVFTTPLCAE 142
             ++   R   ++   +NTDAA          G +IRD   +L AA S       +PL AE
Sbjct: 1290 PVVQYWRPSSSVSLKLNTDAA--CRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAE 1349

Query: 143  AAAVLERLQFARDMDLKCITVLTDSLL 165
               +LE L+FA   +   + V +DSLL
Sbjct: 1350 IRCILEGLKFAAASNFTHLEVESDSLL 1373

BLAST of Lag0032476 vs. NCBI nr
Match: XP_030930858.1 (uncharacterized protein LOC115956689 [Quercus lobata])

HSP 1 Score: 72.8 bits (177), Expect = 3.2e-09
Identity = 50/139 (35.97%), Postives = 71/139 (51.08%), Query Frame = 0

Query: 31  AWTIWNDRNKIVHNLPVPTIEIKCDWIFAYLEEFQ----KVQVASGATSSLYAEFQTLIA 90
           AW IW++RN+I       +  +   W   YLEEFQ    KVQ+ S  +   ++     + 
Sbjct: 13  AWGIWSNRNEIRTGGSQKSASLIVKWTLDYLEEFQVANHKVQITSAESECGWS-----LP 72

Query: 91  RREETIMNTDAAYDAINRKIGIGIVIRDKQGNLKAAHSKSSQVFTTPLCAEAAAVLERLQ 150
                 +N DAA     R +GIG+VIRD  G+++AA SK  +    PL  EA A+ E L+
Sbjct: 73  HAPGFKVNMDAAIFENLRSVGIGVVIRDHLGSVRAAMSKKLRAMLGPLEIEAKAMEEGLR 132

Query: 151 FARDMDLKCITVLTDSLLL 166
           FA D   +  T   DSLL+
Sbjct: 133 FAWDRGFRAATFEGDSLLV 146

BLAST of Lag0032476 vs. NCBI nr
Match: XP_030479177.1 (uncharacterized protein LOC115696415 [Cannabis sativa])

HSP 1 Score: 72.0 bits (175), Expect = 5.4e-09
Identity = 47/149 (31.54%), Postives = 74/149 (49.66%), Query Frame = 0

Query: 16   FCNYQQDDLNLLCVGAWTIWNDRNKIVHN-LPVPTIEIKCDWIFAYLEEF--QKVQVASG 75
            F    ++D+ +L    W +WN RN IV+N   +P  E+  DW   Y  +F   + Q  +G
Sbjct: 1042 FNTLDKNDVGILFCFIWALWNQRNNIVYNQYTLPPKEV-YDWSLNYFFKFLDAQQQHVTG 1101

Query: 76   ATSSLYAEFQTLIARREETIMNTDAAYDAINRKIGIGIVIRDKQGNLKAAHSKSSQVFTT 135
            +  +  +   T I       + TDAA D++NRK  IG+V+ D    +KA  S        
Sbjct: 1102 SQFASNSAQPTQIPPSTRFRIFTDAAIDSVNRKHSIGVVVLDADNAVKAGFSAPFSGLVP 1161

Query: 136  PLCAEAAAVLERLQFARDMDLKCITVLTD 162
            P  AEA A+ + +Q+A+ + L    +LTD
Sbjct: 1162 PAVAEAKAIFQAIQWAQMISLPVDVLLTD 1189

BLAST of Lag0032476 vs. NCBI nr
Match: TXG57115.1 (hypothetical protein EZV62_018428 [Acer yangbiense])

HSP 1 Score: 72.0 bits (175), Expect = 5.4e-09
Identity = 46/148 (31.08%), Postives = 78/148 (52.70%), Query Frame = 0

Query: 20  QQDDLNLLCVGAWTIWNDRNKIVH-NLPVPTIEIKCDWIFAYLEEFQKV----QVASGAT 79
           +++D  L+CV  W IW   N  VH ++ +P  ++   W   ++ +F+      +V  G  
Sbjct: 24  RKEDFGLVCVIIWRIWYYYNGKVHGSVMIPEKDV-VPWAADFITDFRSATGVEKVFLGVN 83

Query: 80  SSLYAEFQTLIARREETIMNTDAAYDAINRKIGIGIVIRDKQGNLKAAHSKSSQVFTTPL 139
           S     ++    R  +  MNTDAA D+   ++G G++IRD  G++ A+ S+    F +P+
Sbjct: 84  SIFGIAWRPPDERSYK--MNTDAAIDSAFGRVGFGVIIRDHVGSVLASSSQFLAAFFSPM 143

Query: 140 CAEAAAVLERLQFARDMDLKCITVLTDS 163
            AEA A+   L+F RD  L   TV+ D+
Sbjct: 144 VAEAMAIFRGLEFTRDSSLLPCTVVLDA 168

BLAST of Lag0032476 vs. NCBI nr
Match: XP_019191714.1 (PREDICTED: uncharacterized protein LOC109186242 [Ipomoea nil])

HSP 1 Score: 70.1 bits (170), Expect = 2.1e-08
Identity = 47/146 (32.19%), Postives = 75/146 (51.37%), Query Frame = 0

Query: 21  QDDLNLLCVGAWTIWNDRN-KIVHNLPVPTIEIKCDWIFAYLEEFQKVQVASGATSSLYA 80
           +D L  + V  W IW  RN KI +N  + + E        YL+++++  + S   +    
Sbjct: 56  RDQLCTVIVCCWEIWRQRNDKIWNNKRIQSAETIVFKASKYLQDWREAALPSLPVNEEST 115

Query: 81  EFQTLIARREETI--MNTDAAYDAINRKIGIGIVIRDKQGNLKAAHSKSSQVFTTPLCAE 140
             Q   ++ +E    +N DAA D+IN K+G G VIRD+ G   AA         +P  AE
Sbjct: 116 TVQPKWSKPQEGFHKLNVDAAIDSINGKMGFGCVIRDEHGTFVAARGIQWDGVFSPREAE 175

Query: 141 AAAVLERLQFARDMDLKCITVLTDSL 164
           A A+ E L + ++++L  ++V TDSL
Sbjct: 176 ATAIREALSWIKELNLNKVSVETDSL 201

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158377.14.9e-1032.65uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_030930858.13.2e-0935.97uncharacterized protein LOC115956689 [Quercus lobata][more]
XP_030479177.15.4e-0931.54uncharacterized protein LOC115696415 [Cannabis sativa][more]
TXG57115.15.4e-0931.08hypothetical protein EZV62_018428 [Acer yangbiense][more]
XP_019191714.12.1e-0832.19PREDICTED: uncharacterized protein LOC109186242 [Ipomoea nil][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 88..166
e-value: 3.6E-9
score: 38.7
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 94..163
e-value: 3.9E-8
score: 33.2
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 93..163
NoneNo IPR availablePANTHERPTHR47074:SF25SUBFAMILY NOT NAMEDcoord: 93..163
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 94..165
e-value: 1.51094E-9
score: 51.1608
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 91..164

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032476.1Lag0032476.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity