Lag0032365 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032365
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein KOKOPELLI isoform X1
Locationchr11: 31313317 .. 31315699 (-)
RNA-Seq ExpressionLag0032365
SyntenyLag0032365
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTTGACGAGTTATATCTTGATCTCCTAGCACTCAGGGAACTATACATCCTCCTCCTAAAGAGCTGTTCGCGAGATGTAAATTCAGAACTTGTAAGCCTCTATGAGATTACTCTTCTCGTTCTCATTCTCATTGTTTTTAACTTTACTAAAGAATGGATACGTATTCTTAATTTGTGAGATTCACACTTAATTGAGTCATCAACATTCATTATTTTCTATGAGAATAGGAAATGTCATTCCCATTCCCTCTTTTGGGGATTCTGCTATTTTTCTTTAACTCTTGTCTTTCCTTTCTAAGGTTTTTTTTTATTTAAATTTTAATTTACATTAATTTAAATTGGTTTTCACTTTAAACAATGGTAAACAATATCAATGAAAATCTATTTCATTCAAATTCCAATCCTAGAGTAATATTAGTCAGTATTTTTTTTTTTTTTTTTTTTTTTGAGAATTAGTAAGTTTAGTCAATATAATTTAAGAAACTTCATTCCAATTCCATAAATGTTTTACCAAGCATGAAACAATTGTACCAAACTTGCAGATTAGTAGTTTTTTGCTGCCCTGTCACTTTTATGTGGATTTAGCATAATAGAGATTGTGAGATAAGACAGTTTGAGTGAGTTTTCTACTTGAATATTTCACTATTGTAGTTCGGCTTTTGTTGTCAAAGCAGCTGGGTGAAAGGGCAGAGACTTTATTGAAGCATTTGCTTGATGATGCTACTGCAGGAATTCTTGAGTTTCACTCAAAGGTTTGTATTTCATTCTGCATAAAACTTTTTTTTTAAACAAAAGAACCATTGGACACAGATACGTATTCAGAAAAATTGTTCAAGTTTACACGTCATGAATGTCTAGTAACATACATTCGTGTCTGAGTTTGTACAGAACTTGGCAACAAACTCTGGCATTTTTTACAACTTTCTACACAAAGATGATAAACAGACAAAGGCACTGGACGAGAAAGTTGCTGAATGGATGGAACATAATCAAACTGCAAGAAAGATGGTAAATCCAGAGATTGAATACAATGCCAAGAGGGCCAGAGCTTCAGCTTCAAATGTTGCCACAAATGACTTATCAAATGACATCAGTTCAGCACTTAGAAGAATTGAACTCCACATTTTATCCTGGAACGTTCCACAAGTCAAAGTAGGAACACAAGAAGCCATATCAGAGAAACTAATTTAGCTTACTGCGGGCTGTCTGTTCTTCAAGGGAATGAGACATTAAAACAGCAGAAAGTTCAGTCAAGGACAGATCACTCAACTTTAAGGACCAGCTTTGCTGAGTCGATTAAAGGCCATAACTTGAGCAGTCAGTTAAGAAGTCATCTTGTTGGTGGACAGAAAGTTAAACCAGTAGTGACAAACCATTGTTCTGAGTTCGTTCATGGATTTAGAATACCTCTGCGTCAAGACAATGATGAGGCGATGAAACCTCCAACAGTTGAAACTCGCATATTTAAACAACACAAACTTGTAAACCCAACGACTCTGATAGATAAATCTGGATGTTCAGTAGGATCCAAGGCAACTGTCAGGTTCGGTTTGAAACTGAATCAAACTCAGATACAAGAAAAGATGATCCAGCATTCATATGGTCGTACGATAATGAGGCCAACTTTGCTTGATCATCCTTCCAGAGAAGTAAGAAAGGAACAAACTCATAACAAGACCCATTTGGCCACTCAGCAAGAATCAAAATTCACAAACTCAGAATCAGAATCAACTTCTTCTTCAAGTTGGACAACTCAACAAACCAGTGAAAGTGAAACCACTTATGACGCTTCTTCCCTAAGTCACCAAGATGGTTCACCAGCAACCGGTTCAGAGGCAAGTAGCCGGTACAGAAGCAGCAGTAGCAGCATTTCAACAAAAGCATTTAAATTCAACCATGGGAAAAAAGAGTCTGAGAGAGCAATAGGACGGTTCAAGAGACTCAAAAACAAACTAGGCCTTATCTTCCACCACCACCATCATCATCACCACCATCATCATCACCACCATCACCACCATAACAGCCATAACTTCATGTGGAAGCAGCTAAGAAAGATCTTCCATCGCACAGATAACAGAAAACTAACAAGTAAAGTAGAAAGATATGGGATGCTAAAGAAAACAGCAATCAGGAGTGTGTCTCGCAAGAACCAAGTTGGGAAGTTTGAGGCACTTGCTGAAGGGCTTCGAAGCCATGTATGGAGATCGAAACCCATGAAAAAGAAAGAGCTTAGGGGGCTGAATTGTGGGAAGAAGAAAGGTGTGAAGAAGTTGCATTGGTGGAAAATGTTTCGTCGTCACCGTGGAGTGAAGTTGCCCAATAAAGGGCGTGTGAAAATAGGGTATGTAAATAGAAAAACACAGCCTCAGGTGGTTTAG

mRNA sequence

ATGGATGTTGACGAGTTATATCTTGATCTCCTAGCACTCAGGGAACTATACATCCTCCTCCTAAAGAGCTGTTCGCGAGATGTAAATTCAGAACTTCTGGGTGAAAGGGCAGAGACTTTATTGAAGCATTTGCTTGATGATGCTACTGCAGGAATTCTTGAGTTTCACTCAAAGAACTTGGCAACAAACTCTGGCATTTTTTACAACTTTCTACACAAAGATGATAAACAGACAAAGGCACTGGACGAGAAAGTTGCTGAATGGATGGAACATAATCAAACTGCAAGAAAGATGGTAAATCCAGAGATTGAATACAATGCCAAGAGGGCCAGAGCTTCAGCTTCAAATGTTGCCACAAATGACTTATCAAATGACATCAGGAATGAGACATTAAAACAGCAGAAAGTTCAGTCAAGGACAGATCACTCAACTTTAAGGACCAGCTTTGCTGAGTCGATTAAAGGCCATAACTTGAGCAGTCAGTTAAGAAGTCATCTTGTTGGTGGACAGAAAGTTAAACCAGTAGTGACAAACCATTGTTCTGAGTTCGTTCATGGATTTAGAATACCTCTGCGTCAAGACAATGATGAGGCGATGAAACCTCCAACAGTTGAAACTCGCATATTTAAACAACACAAACTTGTAAACCCAACGACTCTGATAGATAAATCTGGATGTTCAGTAGGATCCAAGGCAACTGTCAGGTTCGGTTTGAAACTGAATCAAACTCAGATACAAGAAAAGATGATCCAGCATTCATATGGTCGTACGATAATGAGGCCAACTTTGCTTGATCATCCTTCCAGAGAAGTAAGAAAGGAACAAACTCATAACAAGACCCATTTGGCCACTCAGCAAGAATCAAAATTCACAAACTCAGAATCAGAATCAACTTCTTCTTCAAGTTGGACAACTCAACAAACCAGTGAAAGTGAAACCACTTATGACGCTTCTTCCCTAAGTCACCAAGATGGTTCACCAGCAACCGGTTCAGAGGCAAGTAGCCGGTACAGAAGCAGCAGTAGCAGCATTTCAACAAAAGCATTTAAATTCAACCATGGGAAAAAAGAGTCTGAGAGAGCAATAGGACGGTTCAAGAGACTCAAAAACAAACTAGGCCTTATCTTCCACCACCACCATCATCATCACCACCATCATCATCACCACCATCACCACCATAACAGCCATAACTTCATGTGGAAGCAGCTAAGAAAGATCTTCCATCGCACAGATAACAGAAAACTAACAAGTAAAGTAGAAAGATATGGGATGCTAAAGAAAACAGCAATCAGGAGTGTGTCTCGCAAGAACCAAGTTGGGAAGTTTGAGGCACTTGCTGAAGGGCTTCGAAGCCATGTATGGAGATCGAAACCCATGAAAAAGAAAGAGCTTAGGGGGCTGAATTGTGGGAAGAAGAAAGGTGTGAAGAAGTTGCATTGGTGGAAAATGTTTCGTCGTCACCGTGGAGTGAAGTTGCCCAATAAAGGGCGTGTGAAAATAGGGTATGTAAATAGAAAAACACAGCCTCAGGTGGTTTAG

Coding sequence (CDS)

ATGGATGTTGACGAGTTATATCTTGATCTCCTAGCACTCAGGGAACTATACATCCTCCTCCTAAAGAGCTGTTCGCGAGATGTAAATTCAGAACTTCTGGGTGAAAGGGCAGAGACTTTATTGAAGCATTTGCTTGATGATGCTACTGCAGGAATTCTTGAGTTTCACTCAAAGAACTTGGCAACAAACTCTGGCATTTTTTACAACTTTCTACACAAAGATGATAAACAGACAAAGGCACTGGACGAGAAAGTTGCTGAATGGATGGAACATAATCAAACTGCAAGAAAGATGGTAAATCCAGAGATTGAATACAATGCCAAGAGGGCCAGAGCTTCAGCTTCAAATGTTGCCACAAATGACTTATCAAATGACATCAGGAATGAGACATTAAAACAGCAGAAAGTTCAGTCAAGGACAGATCACTCAACTTTAAGGACCAGCTTTGCTGAGTCGATTAAAGGCCATAACTTGAGCAGTCAGTTAAGAAGTCATCTTGTTGGTGGACAGAAAGTTAAACCAGTAGTGACAAACCATTGTTCTGAGTTCGTTCATGGATTTAGAATACCTCTGCGTCAAGACAATGATGAGGCGATGAAACCTCCAACAGTTGAAACTCGCATATTTAAACAACACAAACTTGTAAACCCAACGACTCTGATAGATAAATCTGGATGTTCAGTAGGATCCAAGGCAACTGTCAGGTTCGGTTTGAAACTGAATCAAACTCAGATACAAGAAAAGATGATCCAGCATTCATATGGTCGTACGATAATGAGGCCAACTTTGCTTGATCATCCTTCCAGAGAAGTAAGAAAGGAACAAACTCATAACAAGACCCATTTGGCCACTCAGCAAGAATCAAAATTCACAAACTCAGAATCAGAATCAACTTCTTCTTCAAGTTGGACAACTCAACAAACCAGTGAAAGTGAAACCACTTATGACGCTTCTTCCCTAAGTCACCAAGATGGTTCACCAGCAACCGGTTCAGAGGCAAGTAGCCGGTACAGAAGCAGCAGTAGCAGCATTTCAACAAAAGCATTTAAATTCAACCATGGGAAAAAAGAGTCTGAGAGAGCAATAGGACGGTTCAAGAGACTCAAAAACAAACTAGGCCTTATCTTCCACCACCACCATCATCATCACCACCATCATCATCACCACCATCACCACCATAACAGCCATAACTTCATGTGGAAGCAGCTAAGAAAGATCTTCCATCGCACAGATAACAGAAAACTAACAAGTAAAGTAGAAAGATATGGGATGCTAAAGAAAACAGCAATCAGGAGTGTGTCTCGCAAGAACCAAGTTGGGAAGTTTGAGGCACTTGCTGAAGGGCTTCGAAGCCATGTATGGAGATCGAAACCCATGAAAAAGAAAGAGCTTAGGGGGCTGAATTGTGGGAAGAAGAAAGGTGTGAAGAAGTTGCATTGGTGGAAAATGTTTCGTCGTCACCGTGGAGTGAAGTTGCCCAATAAAGGGCGTGTGAAAATAGGGTATGTAAATAGAAAAACACAGCCTCAGGTGGTTTAG

Protein sequence

MDVDELYLDLLALRELYILLLKSCSRDVNSELLGERAETLLKHLLDDATAGILEFHSKNLATNSGIFYNFLHKDDKQTKALDEKVAEWMEHNQTARKMVNPEIEYNAKRARASASNVATNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAESIKGHNLSSQLRSHLVGGQKVKPVVTNHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDKSGCSVGSKATVRFGLKLNQTQIQEKMIQHSYGRTIMRPTLLDHPSREVRKEQTHNKTHLATQQESKFTNSESESTSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSSISTKAFKFNHGKKESERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHNFMWKQLRKIFHRTDNRKLTSKVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKKKELRGLNCGKKKGVKKLHWWKMFRRHRGVKLPNKGRVKIGYVNRKTQPQVV
Homology
BLAST of Lag0032365 vs. NCBI nr
Match: XP_022996027.1 (uncharacterized protein LOC111491355 isoform X2 [Cucurbita maxima])

HSP 1 Score: 564.3 bits (1453), Expect = 1.1e-156
Identity = 334/516 (64.73%), Postives = 388/516 (75.19%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSEL-LGERAETLLKHLLDDATAGILEFHSKN 60
           M+ DELYLDLLALR+LY  LLK C RD NSEL +G RA+ LLKHLLDDAT G+LEFHSK 
Sbjct: 1   MEADELYLDLLALRQLYFFLLKCCLRDANSELVVGARAKILLKHLLDDATTGLLEFHSKT 60

Query: 61  LATNSGIFYNFLHKDDKQTKALDEKVAEWMEHNQTARKMVNPE-IEYNAKRARASASNVA 120
           LA     FYNFL KDDKQTK LDEKVAEWMEHNQTAR+M NPE IE+  +R RASASNVA
Sbjct: 61  LA-----FYNFLRKDDKQTKPLDEKVAEWMEHNQTARRMANPEKIEHKPRRDRASASNVA 120

Query: 121 TNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAES-IKGHNLSSQLRSHLVGGQKVKPVVT 180
            NDLS+ I N  L++ ++   +     R+  +E+ +  +  S    +     QKVKP+V 
Sbjct: 121 ANDLSSGI-NSALRRIELHILSLQRYTRSHISETKLAYYGQSVNQGNESFNQQKVKPMVA 180

Query: 181 NHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDKSGCSVGSKATVRFG 240
           NHCS+FV+GFRIPL QD DEAM          KQH+LV P TL+DKSGC  GSKAT R  
Sbjct: 181 NHCSKFVNGFRIPLTQDKDEAM----------KQHELVLPPTLMDKSGCPEGSKATARRA 240

Query: 241 LKLNQTQIQEKMIQHSYGRTIMRPTLLDHPSREVRKEQT-HNKTHLATQQESKFTNSESE 300
           +KLN+T IQEK  ++S GR +M+PTL  HPSREVRKEQT HN+ HLA QQES+FTNSES 
Sbjct: 241 MKLNRTWIQEKRSKNSRGRIVMKPTLWHHPSREVRKEQTHHNRRHLAAQQESEFTNSESA 300

Query: 301 STSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSSISTKAFKFNHGKK 360
           S SS +  T QTSESETT D+SS  +Q    ATGSEASS+Y +SSS+I+ KAFKF+HGKK
Sbjct: 301 SCSSPA--TLQTSESETTDDSSSPDNQSSPTATGSEASSQYGNSSSNITRKAFKFSHGKK 360

Query: 361 ESERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHNFMWKQLRKIFHRTDNRKLT 420
           ES  A+GRFK L+NKLGLIFHHH HH HHHHHHHH HNS   MWKQ+R +FHRTD ++LT
Sbjct: 361 ESNGAVGRFKSLRNKLGLIFHHHQHHQHHHHHHHHGHNS---MWKQVRTVFHRTDKKELT 420

Query: 421 SKVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKKKELRGLNCGKKKGVK 480
           SK E+ G L+KT IRSVSR NQVGKF+AL EGLRSHVW+SK MKKKE RGLNCG     K
Sbjct: 421 SKEEKTGKLRKTTIRSVSRNNQVGKFQALPEGLRSHVWKSKAMKKKEQRGLNCG-----K 480

Query: 481 KLHWWKMFRRHRGVKLPNKGRVKIGYVNRKTQPQVV 513
           KLHWWKM RR RGVK PNKGRVKIGYVNRK   +++
Sbjct: 481 KLHWWKMIRRRRGVKFPNKGRVKIGYVNRKPDVKLI 490

BLAST of Lag0032365 vs. NCBI nr
Match: XP_022996025.1 (uncharacterized protein LOC111491355 isoform X1 [Cucurbita maxima] >XP_022996026.1 uncharacterized protein LOC111491355 isoform X1 [Cucurbita maxima])

HSP 1 Score: 564.3 bits (1453), Expect = 1.1e-156
Identity = 334/516 (64.73%), Postives = 388/516 (75.19%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSEL-LGERAETLLKHLLDDATAGILEFHSKN 60
           M+ DELYLDLLALR+LY  LLK C RD NSEL +G RA+ LLKHLLDDAT G+LEFHSK 
Sbjct: 45  MEADELYLDLLALRQLYFFLLKCCLRDANSELVVGARAKILLKHLLDDATTGLLEFHSKT 104

Query: 61  LATNSGIFYNFLHKDDKQTKALDEKVAEWMEHNQTARKMVNPE-IEYNAKRARASASNVA 120
           LA     FYNFL KDDKQTK LDEKVAEWMEHNQTAR+M NPE IE+  +R RASASNVA
Sbjct: 105 LA-----FYNFLRKDDKQTKPLDEKVAEWMEHNQTARRMANPEKIEHKPRRDRASASNVA 164

Query: 121 TNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAES-IKGHNLSSQLRSHLVGGQKVKPVVT 180
            NDLS+ I N  L++ ++   +     R+  +E+ +  +  S    +     QKVKP+V 
Sbjct: 165 ANDLSSGI-NSALRRIELHILSLQRYTRSHISETKLAYYGQSVNQGNESFNQQKVKPMVA 224

Query: 181 NHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDKSGCSVGSKATVRFG 240
           NHCS+FV+GFRIPL QD DEAM          KQH+LV P TL+DKSGC  GSKAT R  
Sbjct: 225 NHCSKFVNGFRIPLTQDKDEAM----------KQHELVLPPTLMDKSGCPEGSKATARRA 284

Query: 241 LKLNQTQIQEKMIQHSYGRTIMRPTLLDHPSREVRKEQT-HNKTHLATQQESKFTNSESE 300
           +KLN+T IQEK  ++S GR +M+PTL  HPSREVRKEQT HN+ HLA QQES+FTNSES 
Sbjct: 285 MKLNRTWIQEKRSKNSRGRIVMKPTLWHHPSREVRKEQTHHNRRHLAAQQESEFTNSESA 344

Query: 301 STSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSSISTKAFKFNHGKK 360
           S SS +  T QTSESETT D+SS  +Q    ATGSEASS+Y +SSS+I+ KAFKF+HGKK
Sbjct: 345 SCSSPA--TLQTSESETTDDSSSPDNQSSPTATGSEASSQYGNSSSNITRKAFKFSHGKK 404

Query: 361 ESERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHNFMWKQLRKIFHRTDNRKLT 420
           ES  A+GRFK L+NKLGLIFHHH HH HHHHHHHH HNS   MWKQ+R +FHRTD ++LT
Sbjct: 405 ESNGAVGRFKSLRNKLGLIFHHHQHHQHHHHHHHHGHNS---MWKQVRTVFHRTDKKELT 464

Query: 421 SKVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKKKELRGLNCGKKKGVK 480
           SK E+ G L+KT IRSVSR NQVGKF+AL EGLRSHVW+SK MKKKE RGLNCG     K
Sbjct: 465 SKEEKTGKLRKTTIRSVSRNNQVGKFQALPEGLRSHVWKSKAMKKKEQRGLNCG-----K 524

Query: 481 KLHWWKMFRRHRGVKLPNKGRVKIGYVNRKTQPQVV 513
           KLHWWKM RR RGVK PNKGRVKIGYVNRK   +++
Sbjct: 525 KLHWWKMIRRRRGVKFPNKGRVKIGYVNRKPDVKLI 534

BLAST of Lag0032365 vs. NCBI nr
Match: XP_022958322.1 (uncharacterized protein LOC111459571 isoform X2 [Cucurbita moschata] >XP_022958323.1 uncharacterized protein LOC111459571 isoform X2 [Cucurbita moschata])

HSP 1 Score: 547.7 bits (1410), Expect = 1.0e-151
Identity = 320/515 (62.14%), Postives = 378/515 (73.40%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSEL-LGERAETLLKHLLDDATAGILEFHSKN 60
           M+ DELYLDLLALR+LY+ LLK C RD NSEL +G RA+ L KHLLDDAT G+LEFHSK 
Sbjct: 1   MEADELYLDLLALRQLYVFLLKCCLRDANSELVVGARAKILFKHLLDDATTGLLEFHSKT 60

Query: 61  LATNSGIFYNFLHKDDKQTKALDEKVAEWMEHNQTARKMVNPE-IEYNAKRARASASNVA 120
           L      FYNFL KDDKQTK LDEKVAEWMEHNQTAR M NPE IE+   R RASASNVA
Sbjct: 61  LP-----FYNFLRKDDKQTKPLDEKVAEWMEHNQTARTMANPEKIEHKPGRDRASASNVA 120

Query: 121 TNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAES-IKGHNLSSQLRSHLVGGQKVKPVVT 180
            NDLS+ I +  L++ ++   +     R+  +E+ +  +  S    +  +  QKVKP+V 
Sbjct: 121 ANDLSSGI-SSALRRIELHILSLQRYTRSHISETKLAYYGQSVHQGNESLNHQKVKPMVA 180

Query: 181 NHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDKSGCSVGSKATVRFG 240
           NHCS+FVHGFRIPL QD +EAM          KQH+L  P TL+DKSGC  GSKAT R  
Sbjct: 181 NHCSKFVHGFRIPLTQDKNEAM----------KQHELALPPTLMDKSGCPEGSKATARRA 240

Query: 241 LKLNQTQIQEKMIQHSYGRTIMRPTLLDHPSREVRKEQTHNKTHLATQQESKFTNSESES 300
           +KLN+T IQEK  ++S GR +MRPTL             HNKTHLA QQES++TNSESES
Sbjct: 241 MKLNRTWIQEKRSKNSRGRIVMRPTL------------WHNKTHLAAQQESEYTNSESES 300

Query: 301 TSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSSISTKAFKFNHGKKE 360
             SSS  T+QTSESETT D+SS   Q   PATGSEASS+  +SSS+IS +AFKF+HGKKE
Sbjct: 301 APSSSPATRQTSESETTADSSSPGDQSSPPATGSEASSQCGNSSSNISREAFKFSHGKKE 360

Query: 361 SERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHNFMWKQLRKIFHRTDNRKLTS 420
           S++A+GRFK L+NKLGLIF         HHHHHH+HN HN MWKQ+R++FHRT  ++LTS
Sbjct: 361 SKKAVGRFKSLRNKLGLIF---------HHHHHHYHNGHNSMWKQVRRMFHRTGKKELTS 420

Query: 421 KVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKKKELRGLNCGKKKGVKK 480
           K E+ GML+KT IRSVSR NQVGKF+ALAEGLRSHVW+SK MKKKE RGLNCGK  G KK
Sbjct: 421 KEEKNGMLRKTTIRSVSRNNQVGKFQALAEGLRSHVWKSKAMKKKEQRGLNCGKTNGGKK 478

Query: 481 LHWWKMFRRHRGVKLPNKGRVKIGYVNRKTQPQVV 513
           LHWWKM RR RGVKLPNKGRVKIGYVN+K   +++
Sbjct: 481 LHWWKMIRRRRGVKLPNKGRVKIGYVNKKPHVKII 478

BLAST of Lag0032365 vs. NCBI nr
Match: XP_022958321.1 (uncharacterized protein LOC111459571 isoform X1 [Cucurbita moschata])

HSP 1 Score: 547.7 bits (1410), Expect = 1.0e-151
Identity = 320/515 (62.14%), Postives = 378/515 (73.40%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSEL-LGERAETLLKHLLDDATAGILEFHSKN 60
           M+ DELYLDLLALR+LY+ LLK C RD NSEL +G RA+ L KHLLDDAT G+LEFHSK 
Sbjct: 26  MEADELYLDLLALRQLYVFLLKCCLRDANSELVVGARAKILFKHLLDDATTGLLEFHSKT 85

Query: 61  LATNSGIFYNFLHKDDKQTKALDEKVAEWMEHNQTARKMVNPE-IEYNAKRARASASNVA 120
           L      FYNFL KDDKQTK LDEKVAEWMEHNQTAR M NPE IE+   R RASASNVA
Sbjct: 86  LP-----FYNFLRKDDKQTKPLDEKVAEWMEHNQTARTMANPEKIEHKPGRDRASASNVA 145

Query: 121 TNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAES-IKGHNLSSQLRSHLVGGQKVKPVVT 180
            NDLS+ I +  L++ ++   +     R+  +E+ +  +  S    +  +  QKVKP+V 
Sbjct: 146 ANDLSSGI-SSALRRIELHILSLQRYTRSHISETKLAYYGQSVHQGNESLNHQKVKPMVA 205

Query: 181 NHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDKSGCSVGSKATVRFG 240
           NHCS+FVHGFRIPL QD +EAM          KQH+L  P TL+DKSGC  GSKAT R  
Sbjct: 206 NHCSKFVHGFRIPLTQDKNEAM----------KQHELALPPTLMDKSGCPEGSKATARRA 265

Query: 241 LKLNQTQIQEKMIQHSYGRTIMRPTLLDHPSREVRKEQTHNKTHLATQQESKFTNSESES 300
           +KLN+T IQEK  ++S GR +MRPTL             HNKTHLA QQES++TNSESES
Sbjct: 266 MKLNRTWIQEKRSKNSRGRIVMRPTL------------WHNKTHLAAQQESEYTNSESES 325

Query: 301 TSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSSISTKAFKFNHGKKE 360
             SSS  T+QTSESETT D+SS   Q   PATGSEASS+  +SSS+IS +AFKF+HGKKE
Sbjct: 326 APSSSPATRQTSESETTADSSSPGDQSSPPATGSEASSQCGNSSSNISREAFKFSHGKKE 385

Query: 361 SERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHNFMWKQLRKIFHRTDNRKLTS 420
           S++A+GRFK L+NKLGLIF         HHHHHH+HN HN MWKQ+R++FHRT  ++LTS
Sbjct: 386 SKKAVGRFKSLRNKLGLIF---------HHHHHHYHNGHNSMWKQVRRMFHRTGKKELTS 445

Query: 421 KVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKKKELRGLNCGKKKGVKK 480
           K E+ GML+KT IRSVSR NQVGKF+ALAEGLRSHVW+SK MKKKE RGLNCGK  G KK
Sbjct: 446 KEEKNGMLRKTTIRSVSRNNQVGKFQALAEGLRSHVWKSKAMKKKEQRGLNCGKTNGGKK 503

Query: 481 LHWWKMFRRHRGVKLPNKGRVKIGYVNRKTQPQVV 513
           LHWWKM RR RGVKLPNKGRVKIGYVN+K   +++
Sbjct: 506 LHWWKMIRRRRGVKLPNKGRVKIGYVNKKPHVKII 503

BLAST of Lag0032365 vs. NCBI nr
Match: XP_038877121.1 (protein KOKOPELLI-like isoform X1 [Benincasa hispida])

HSP 1 Score: 543.5 bits (1399), Expect = 2.0e-150
Identity = 340/555 (61.26%), Postives = 391/555 (70.45%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSELLGERAETLLKHLLDDATAGILEFHSKNL 60
           MDVD+LYLDLLALRELYILLLKSC  D NSELL ERA+ LLKHLLDDATAG+LEF S +L
Sbjct: 31  MDVDKLYLDLLALRELYILLLKSCLGDANSELLDERAQILLKHLLDDATAGVLEFLSNDL 90

Query: 61  ATNSGIFYNFLHKDDKQTKALDEKVAEWMEHNQTARKMVNPEIEYNAKRARASASNVATN 120
           ATNS IF NFLHKDDKQ K L +KV EWM+HNQT RKM NPEI     R RASASNVA N
Sbjct: 91  ATNSNIFDNFLHKDDKQVKPLADKVPEWMKHNQTRRKMGNPEI-----RDRASASNVAIN 150

Query: 121 DLSNDIR---------------------------------NETLKQQKVQSRTDHSTLRT 180
           +LS+ I                                  NE+L QQ V  RT  STLR+
Sbjct: 151 NLSHSISSALRRIELHILSLQHCTSQRRKTRCHWQSVLQWNESLNQQNVHPRTGPSTLRS 210

Query: 181 SFAESIKGHNLSSQLRSHLVGGQ-KVKPVVTNHCSEFVHGFRIPLRQDNDEAMKPPTVET 240
            F + IKG       R H VG Q KVKP   NHCSE+VHGFRIPL Q NDEAMKP T+ET
Sbjct: 211 RFTKPIKG-------RGHFVGEQKKVKPKTANHCSEYVHGFRIPLSQTNDEAMKPLTIET 270

Query: 241 RIFKQHKLVNPTTLIDKSG-CSVGSKATVRFGLKLNQTQIQE-KMIQHSYGRTIMRPTLL 300
            I KQHK+VNP TLIDKSG  SVGSKAT R  +KLNQT  Q+ K  Q+SYG+ +M PTLL
Sbjct: 271 HITKQHKVVNPMTLIDKSGYTSVGSKATFRPAMKLNQTSKQQAKRNQNSYGQMVMGPTLL 330

Query: 301 D-HPSREVRKEQTHNKTHL-ATQQESKFTNSE--SESTSSSSWTTQQTSESETT-----Y 360
           D HPS+E R E+ ++KTHL ATQQES+FT+SE  S S+SSSSWTTQ+TS SET       
Sbjct: 331 DHHPSKETRNERINSKTHLAATQQESEFTSSEFQSASSSSSSWTTQETSVSETVANDGDS 390

Query: 361 DASSLSHQDGSPATGSEASSRYRSSSSSISTKAFKFNHGKKESERAIGRFKRLKNKLGLI 420
           + SS SHQD   +T S++SS          TK F    GK ES++ +GRFKRLKNKLG++
Sbjct: 391 NPSSPSHQDDPLSTDSKSSS---------LTKTFYIKQGKTESKKVLGRFKRLKNKLGVV 450

Query: 421 FHHHHHHHHHHHHHHHHHNSHNFMWK-QLRKIFHRTDNRK-LTSKVERYGMLKKTAIRSV 480
           F      HHHHHHHHHHHNS+NFMWK QLRKIFH  DN++ L SK +    +KK AIR+V
Sbjct: 451 F------HHHHHHHHHHHNSNNFMWKQQLRKIFHSRDNKRLLVSKEDGNEKVKKRAIRNV 510

Query: 481 SRKNQVGKFEALAEGLRSHVWRSKPMKKKELRGLNCGKKKGVKKLHWWKMFRRHRGVKLP 509
             KNQVGKF+ALAEGLRSHVWRSK MK+K ++G+ CG KKGVKKLHWWKMFR  RGV+LP
Sbjct: 511 CYKNQVGKFQALAEGLRSHVWRSKAMKRKGVKGMKCG-KKGVKKLHWWKMFRNRRGVRLP 557

BLAST of Lag0032365 vs. ExPASy Swiss-Prot
Match: Q9FFP2 (Protein KOKOPELLI OS=Arabidopsis thaliana OX=3702 GN=KPL PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.2e-15
Identity = 140/520 (26.92%), Postives = 215/520 (41.35%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSEL--LGERAETLLKHLLDDATAGILEFHSK 60
           M+ + +  +L +LR LY LL+ +   +   E   L +  + LLK LLD A+       S 
Sbjct: 1   MEPNNVERNLQSLRRLYSLLVANARNEYIPEAYKLDDNTQFLLKRLLDFASHEHFVTQSN 60

Query: 61  NLATNSGIFYNFLHKDDKQTKALDEK----VAEWMEHNQTARKMVNPEIEYNAKRARASA 120
            LAT   +F   +    K +   D       A   + N +    V+  +E      R   
Sbjct: 61  LLATQLRVFPKTVLHGAKPSNVADSSETTPPAMLSQINGSHITRVSKPLEAKGTLQRDLR 120

Query: 121 SNVATNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAESIKGHNLSSQLRSHLVGGQKVKP 180
            +   ++ S D+  E +     Q  T  S L  SF  S     + S  R+  V      P
Sbjct: 121 VDQIRSNPSKDVLTEEVVDAIEQIDTQLSAL--SFVSS----RVDSDERTRSVKSFVTPP 180

Query: 181 VVTNHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDKSGCSVGSKATV 240
             T  C +      +P  + N   +   +V +         +    +      +  +A  
Sbjct: 181 --TEECRQNSQA-SMPCLRSNYMTVSQKSVISGAEVTFPYNDQLVRVTSPPQPLPPRAVS 240

Query: 241 RFGLKLNQTQIQEKMIQHSYGRTIMRPTLLDH-------PSREVRKEQTHNKTHLATQQE 300
            F       +  +KM        IM+PTL+D         S E   +QT + T   ++ E
Sbjct: 241 GFKKPNQSNRASQKM-------PIMKPTLMDQETETFDDDSSETEADQTPSATGSESEDE 300

Query: 301 SKFT----NSESESTSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSS 360
              T    + E+ S+S S W TQ  +++E+  ++S     D S +           S+S 
Sbjct: 301 EVSTSQEYSGETGSSSGSEWETQAENDTESKSESSYPPQNDDSVS---------EVSTSP 360

Query: 361 ISTKAFKFNHGKKESERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHNFMWKQL 420
             T         K+    +GRFKR+KNK+G IFHHHHHHHHHHHHH     S    W +L
Sbjct: 361 PHTDRDTSREPGKQRRNVMGRFKRIKNKIGQIFHHHHHHHHHHHHHDKEKPS---AWNKL 420

Query: 421 RKIFHRTDNRKLTSKVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKKKE 480
           +  FH     K  SK  +  M +   + +  +++Q G F AL EGL  H   SK  K + 
Sbjct: 421 QSKFHHKHQEK--SKERKRPMSESKGLTTHKQQHQGGHFHALVEGLVRHRKHSKKQKHQ- 480

Query: 481 LRGLNCGKKKGVKKLHWWKMFRRHR--GVKLPNKGRVKIG 502
                   K   KK  WWK+ ++ +  GVK+P +GRVK+G
Sbjct: 481 -------LKSDAKKTEWWKLLKKRQGGGVKIPKRGRVKLG 482

BLAST of Lag0032365 vs. ExPASy TrEMBL
Match: A0A6J1K5J4 (uncharacterized protein LOC111491355 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491355 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 5.2e-157
Identity = 334/516 (64.73%), Postives = 388/516 (75.19%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSEL-LGERAETLLKHLLDDATAGILEFHSKN 60
           M+ DELYLDLLALR+LY  LLK C RD NSEL +G RA+ LLKHLLDDAT G+LEFHSK 
Sbjct: 45  MEADELYLDLLALRQLYFFLLKCCLRDANSELVVGARAKILLKHLLDDATTGLLEFHSKT 104

Query: 61  LATNSGIFYNFLHKDDKQTKALDEKVAEWMEHNQTARKMVNPE-IEYNAKRARASASNVA 120
           LA     FYNFL KDDKQTK LDEKVAEWMEHNQTAR+M NPE IE+  +R RASASNVA
Sbjct: 105 LA-----FYNFLRKDDKQTKPLDEKVAEWMEHNQTARRMANPEKIEHKPRRDRASASNVA 164

Query: 121 TNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAES-IKGHNLSSQLRSHLVGGQKVKPVVT 180
            NDLS+ I N  L++ ++   +     R+  +E+ +  +  S    +     QKVKP+V 
Sbjct: 165 ANDLSSGI-NSALRRIELHILSLQRYTRSHISETKLAYYGQSVNQGNESFNQQKVKPMVA 224

Query: 181 NHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDKSGCSVGSKATVRFG 240
           NHCS+FV+GFRIPL QD DEAM          KQH+LV P TL+DKSGC  GSKAT R  
Sbjct: 225 NHCSKFVNGFRIPLTQDKDEAM----------KQHELVLPPTLMDKSGCPEGSKATARRA 284

Query: 241 LKLNQTQIQEKMIQHSYGRTIMRPTLLDHPSREVRKEQT-HNKTHLATQQESKFTNSESE 300
           +KLN+T IQEK  ++S GR +M+PTL  HPSREVRKEQT HN+ HLA QQES+FTNSES 
Sbjct: 285 MKLNRTWIQEKRSKNSRGRIVMKPTLWHHPSREVRKEQTHHNRRHLAAQQESEFTNSESA 344

Query: 301 STSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSSISTKAFKFNHGKK 360
           S SS +  T QTSESETT D+SS  +Q    ATGSEASS+Y +SSS+I+ KAFKF+HGKK
Sbjct: 345 SCSSPA--TLQTSESETTDDSSSPDNQSSPTATGSEASSQYGNSSSNITRKAFKFSHGKK 404

Query: 361 ESERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHNFMWKQLRKIFHRTDNRKLT 420
           ES  A+GRFK L+NKLGLIFHHH HH HHHHHHHH HNS   MWKQ+R +FHRTD ++LT
Sbjct: 405 ESNGAVGRFKSLRNKLGLIFHHHQHHQHHHHHHHHGHNS---MWKQVRTVFHRTDKKELT 464

Query: 421 SKVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKKKELRGLNCGKKKGVK 480
           SK E+ G L+KT IRSVSR NQVGKF+AL EGLRSHVW+SK MKKKE RGLNCG     K
Sbjct: 465 SKEEKTGKLRKTTIRSVSRNNQVGKFQALPEGLRSHVWKSKAMKKKEQRGLNCG-----K 524

Query: 481 KLHWWKMFRRHRGVKLPNKGRVKIGYVNRKTQPQVV 513
           KLHWWKM RR RGVK PNKGRVKIGYVNRK   +++
Sbjct: 525 KLHWWKMIRRRRGVKFPNKGRVKIGYVNRKPDVKLI 534

BLAST of Lag0032365 vs. ExPASy TrEMBL
Match: A0A6J1K0S1 (uncharacterized protein LOC111491355 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491355 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 5.2e-157
Identity = 334/516 (64.73%), Postives = 388/516 (75.19%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSEL-LGERAETLLKHLLDDATAGILEFHSKN 60
           M+ DELYLDLLALR+LY  LLK C RD NSEL +G RA+ LLKHLLDDAT G+LEFHSK 
Sbjct: 1   MEADELYLDLLALRQLYFFLLKCCLRDANSELVVGARAKILLKHLLDDATTGLLEFHSKT 60

Query: 61  LATNSGIFYNFLHKDDKQTKALDEKVAEWMEHNQTARKMVNPE-IEYNAKRARASASNVA 120
           LA     FYNFL KDDKQTK LDEKVAEWMEHNQTAR+M NPE IE+  +R RASASNVA
Sbjct: 61  LA-----FYNFLRKDDKQTKPLDEKVAEWMEHNQTARRMANPEKIEHKPRRDRASASNVA 120

Query: 121 TNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAES-IKGHNLSSQLRSHLVGGQKVKPVVT 180
            NDLS+ I N  L++ ++   +     R+  +E+ +  +  S    +     QKVKP+V 
Sbjct: 121 ANDLSSGI-NSALRRIELHILSLQRYTRSHISETKLAYYGQSVNQGNESFNQQKVKPMVA 180

Query: 181 NHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDKSGCSVGSKATVRFG 240
           NHCS+FV+GFRIPL QD DEAM          KQH+LV P TL+DKSGC  GSKAT R  
Sbjct: 181 NHCSKFVNGFRIPLTQDKDEAM----------KQHELVLPPTLMDKSGCPEGSKATARRA 240

Query: 241 LKLNQTQIQEKMIQHSYGRTIMRPTLLDHPSREVRKEQT-HNKTHLATQQESKFTNSESE 300
           +KLN+T IQEK  ++S GR +M+PTL  HPSREVRKEQT HN+ HLA QQES+FTNSES 
Sbjct: 241 MKLNRTWIQEKRSKNSRGRIVMKPTLWHHPSREVRKEQTHHNRRHLAAQQESEFTNSESA 300

Query: 301 STSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSSISTKAFKFNHGKK 360
           S SS +  T QTSESETT D+SS  +Q    ATGSEASS+Y +SSS+I+ KAFKF+HGKK
Sbjct: 301 SCSSPA--TLQTSESETTDDSSSPDNQSSPTATGSEASSQYGNSSSNITRKAFKFSHGKK 360

Query: 361 ESERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHNFMWKQLRKIFHRTDNRKLT 420
           ES  A+GRFK L+NKLGLIFHHH HH HHHHHHHH HNS   MWKQ+R +FHRTD ++LT
Sbjct: 361 ESNGAVGRFKSLRNKLGLIFHHHQHHQHHHHHHHHGHNS---MWKQVRTVFHRTDKKELT 420

Query: 421 SKVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKKKELRGLNCGKKKGVK 480
           SK E+ G L+KT IRSVSR NQVGKF+AL EGLRSHVW+SK MKKKE RGLNCG     K
Sbjct: 421 SKEEKTGKLRKTTIRSVSRNNQVGKFQALPEGLRSHVWKSKAMKKKEQRGLNCG-----K 480

Query: 481 KLHWWKMFRRHRGVKLPNKGRVKIGYVNRKTQPQVV 513
           KLHWWKM RR RGVK PNKGRVKIGYVNRK   +++
Sbjct: 481 KLHWWKMIRRRRGVKFPNKGRVKIGYVNRKPDVKLI 490

BLAST of Lag0032365 vs. ExPASy TrEMBL
Match: A0A6J1H2T7 (uncharacterized protein LOC111459571 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459571 PE=4 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 5.0e-152
Identity = 320/515 (62.14%), Postives = 378/515 (73.40%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSEL-LGERAETLLKHLLDDATAGILEFHSKN 60
           M+ DELYLDLLALR+LY+ LLK C RD NSEL +G RA+ L KHLLDDAT G+LEFHSK 
Sbjct: 1   MEADELYLDLLALRQLYVFLLKCCLRDANSELVVGARAKILFKHLLDDATTGLLEFHSKT 60

Query: 61  LATNSGIFYNFLHKDDKQTKALDEKVAEWMEHNQTARKMVNPE-IEYNAKRARASASNVA 120
           L      FYNFL KDDKQTK LDEKVAEWMEHNQTAR M NPE IE+   R RASASNVA
Sbjct: 61  LP-----FYNFLRKDDKQTKPLDEKVAEWMEHNQTARTMANPEKIEHKPGRDRASASNVA 120

Query: 121 TNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAES-IKGHNLSSQLRSHLVGGQKVKPVVT 180
            NDLS+ I +  L++ ++   +     R+  +E+ +  +  S    +  +  QKVKP+V 
Sbjct: 121 ANDLSSGI-SSALRRIELHILSLQRYTRSHISETKLAYYGQSVHQGNESLNHQKVKPMVA 180

Query: 181 NHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDKSGCSVGSKATVRFG 240
           NHCS+FVHGFRIPL QD +EAM          KQH+L  P TL+DKSGC  GSKAT R  
Sbjct: 181 NHCSKFVHGFRIPLTQDKNEAM----------KQHELALPPTLMDKSGCPEGSKATARRA 240

Query: 241 LKLNQTQIQEKMIQHSYGRTIMRPTLLDHPSREVRKEQTHNKTHLATQQESKFTNSESES 300
           +KLN+T IQEK  ++S GR +MRPTL             HNKTHLA QQES++TNSESES
Sbjct: 241 MKLNRTWIQEKRSKNSRGRIVMRPTL------------WHNKTHLAAQQESEYTNSESES 300

Query: 301 TSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSSISTKAFKFNHGKKE 360
             SSS  T+QTSESETT D+SS   Q   PATGSEASS+  +SSS+IS +AFKF+HGKKE
Sbjct: 301 APSSSPATRQTSESETTADSSSPGDQSSPPATGSEASSQCGNSSSNISREAFKFSHGKKE 360

Query: 361 SERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHNFMWKQLRKIFHRTDNRKLTS 420
           S++A+GRFK L+NKLGLIF         HHHHHH+HN HN MWKQ+R++FHRT  ++LTS
Sbjct: 361 SKKAVGRFKSLRNKLGLIF---------HHHHHHYHNGHNSMWKQVRRMFHRTGKKELTS 420

Query: 421 KVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKKKELRGLNCGKKKGVKK 480
           K E+ GML+KT IRSVSR NQVGKF+ALAEGLRSHVW+SK MKKKE RGLNCGK  G KK
Sbjct: 421 KEEKNGMLRKTTIRSVSRNNQVGKFQALAEGLRSHVWKSKAMKKKEQRGLNCGKTNGGKK 478

Query: 481 LHWWKMFRRHRGVKLPNKGRVKIGYVNRKTQPQVV 513
           LHWWKM RR RGVKLPNKGRVKIGYVN+K   +++
Sbjct: 481 LHWWKMIRRRRGVKLPNKGRVKIGYVNKKPHVKII 478

BLAST of Lag0032365 vs. ExPASy TrEMBL
Match: A0A6J1H1S0 (uncharacterized protein LOC111459571 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459571 PE=4 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 5.0e-152
Identity = 320/515 (62.14%), Postives = 378/515 (73.40%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSEL-LGERAETLLKHLLDDATAGILEFHSKN 60
           M+ DELYLDLLALR+LY+ LLK C RD NSEL +G RA+ L KHLLDDAT G+LEFHSK 
Sbjct: 26  MEADELYLDLLALRQLYVFLLKCCLRDANSELVVGARAKILFKHLLDDATTGLLEFHSKT 85

Query: 61  LATNSGIFYNFLHKDDKQTKALDEKVAEWMEHNQTARKMVNPE-IEYNAKRARASASNVA 120
           L      FYNFL KDDKQTK LDEKVAEWMEHNQTAR M NPE IE+   R RASASNVA
Sbjct: 86  LP-----FYNFLRKDDKQTKPLDEKVAEWMEHNQTARTMANPEKIEHKPGRDRASASNVA 145

Query: 121 TNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAES-IKGHNLSSQLRSHLVGGQKVKPVVT 180
            NDLS+ I +  L++ ++   +     R+  +E+ +  +  S    +  +  QKVKP+V 
Sbjct: 146 ANDLSSGI-SSALRRIELHILSLQRYTRSHISETKLAYYGQSVHQGNESLNHQKVKPMVA 205

Query: 181 NHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDKSGCSVGSKATVRFG 240
           NHCS+FVHGFRIPL QD +EAM          KQH+L  P TL+DKSGC  GSKAT R  
Sbjct: 206 NHCSKFVHGFRIPLTQDKNEAM----------KQHELALPPTLMDKSGCPEGSKATARRA 265

Query: 241 LKLNQTQIQEKMIQHSYGRTIMRPTLLDHPSREVRKEQTHNKTHLATQQESKFTNSESES 300
           +KLN+T IQEK  ++S GR +MRPTL             HNKTHLA QQES++TNSESES
Sbjct: 266 MKLNRTWIQEKRSKNSRGRIVMRPTL------------WHNKTHLAAQQESEYTNSESES 325

Query: 301 TSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSSISTKAFKFNHGKKE 360
             SSS  T+QTSESETT D+SS   Q   PATGSEASS+  +SSS+IS +AFKF+HGKKE
Sbjct: 326 APSSSPATRQTSESETTADSSSPGDQSSPPATGSEASSQCGNSSSNISREAFKFSHGKKE 385

Query: 361 SERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHNFMWKQLRKIFHRTDNRKLTS 420
           S++A+GRFK L+NKLGLIF         HHHHHH+HN HN MWKQ+R++FHRT  ++LTS
Sbjct: 386 SKKAVGRFKSLRNKLGLIF---------HHHHHHYHNGHNSMWKQVRRMFHRTGKKELTS 445

Query: 421 KVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKKKELRGLNCGKKKGVKK 480
           K E+ GML+KT IRSVSR NQVGKF+ALAEGLRSHVW+SK MKKKE RGLNCGK  G KK
Sbjct: 446 KEEKNGMLRKTTIRSVSRNNQVGKFQALAEGLRSHVWKSKAMKKKEQRGLNCGKTNGGKK 503

Query: 481 LHWWKMFRRHRGVKLPNKGRVKIGYVNRKTQPQVV 513
           LHWWKM RR RGVKLPNKGRVKIGYVN+K   +++
Sbjct: 506 LHWWKMIRRRRGVKLPNKGRVKIGYVNKKPHVKII 503

BLAST of Lag0032365 vs. ExPASy TrEMBL
Match: A0A6J1DNR3 (protein KOKOPELLI isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022084 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 7.5e-140
Identity = 316/531 (59.51%), Postives = 365/531 (68.74%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSELLGERAETLLKHLLDDATAGILEFHSKNL 60
           M+V+ELYLDLLALRELYILLLKSC RD NSELL ERA+ LLKHLLDDATA I++FHSK  
Sbjct: 1   MEVNELYLDLLALRELYILLLKSCLRDANSELLDERAQILLKHLLDDATAEIVQFHSK-- 60

Query: 61  ATNSGIFYNFLHKDDKQTKALDEKVAEWMEHNQTARKMVN--------------PEIEY- 120
                            TK ++EKVAEWME+NQ+ RK  N                IE+ 
Sbjct: 61  -----------------TKPVEEKVAEWMEYNQSTRKTGNVAANDLSNGIGLALRRIEFH 120

Query: 121 --NAKRARASASNVATNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAESIKGHNLSSQLR 180
             + +   + + N  ++     + N  L QQKVQSR DHS L+   AE I G        
Sbjct: 121 ILSLQHYTSQSRNTRSHINGAKLSNSPLDQQKVQSRMDHSNLKARVAEPING-------- 180

Query: 181 SHLVGGQKVKPVVTNHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDK 240
                          HCSEFVHGFR+PL QDN EAMKPP V T++ KQ+K++NP  LIDK
Sbjct: 181 ---------------HCSEFVHGFRVPLSQDNVEAMKPPNVGTQVSKQNKVINPVILIDK 240

Query: 241 SGCSVGSKATVRFGLKLNQTQIQEKMIQHSYGRTIMRPTLLDHPSREVRKEQTHNKTHLA 300
           S CSVGSKATVR    +N+TQI E+  Q+  G  IMRPTLL+           H KT + 
Sbjct: 241 SRCSVGSKATVR---SVNRTQIHERRCQNLPGHMIMRPTLLN-----------HMKTRMP 300

Query: 301 TQQESKFTNSESESTSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSS 360
           TQQES+FTNSESES SSSSW TQQTSE+ETT   SS SHQ+  PATGSE SSRYR  SS 
Sbjct: 301 TQQESEFTNSESESVSSSSWATQQTSETETTDYPSSSSHQEDQPATGSEVSSRYR--SSR 360

Query: 361 ISTKAFKFNHGKKESERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHN--FMWK 420
           IS+KAF+ +HGKK S++AIGRFKRL+NKLGLIF     HHHHHHHHHHHHNSHN  FMWK
Sbjct: 361 ISSKAFRISHGKKGSKKAIGRFKRLRNKLGLIF-----HHHHHHHHHHHHNSHNNFFMWK 420

Query: 421 QLRKIFHRTDNRKLTSKVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKK 480
           QLRKIFH TD +++TSK  R+  LKKTAIRSVSRKNQVG+F+ALAEGLRSHVW+   MKK
Sbjct: 421 QLRKIFHGTDKKRVTSK-GRHETLKKTAIRSVSRKNQVGRFQALAEGLRSHVWKPTAMKK 466

Query: 481 KELRGLNCGKKKGVKKLHWWKMFRRHRGVKLPNKGRVKIGYVNRKTQPQVV 513
           KELR    G KKGVKKLHWW+MF R RGVKLPNKGRVKIGYVNRK Q ++V
Sbjct: 481 KELRKPRLG-KKGVKKLHWWRMFCRRRGVKLPNKGRVKIGYVNRKPQHKIV 466

BLAST of Lag0032365 vs. TAIR 10
Match: AT5G63720.1 (kokopelli )

HSP 1 Score: 84.3 bits (207), Expect = 3.0e-16
Identity = 140/520 (26.92%), Postives = 215/520 (41.35%), Query Frame = 0

Query: 1   MDVDELYLDLLALRELYILLLKSCSRDVNSEL--LGERAETLLKHLLDDATAGILEFHSK 60
           M+ + +  +L +LR LY LL+ +   +   E   L +  + LLK LLD A+       S 
Sbjct: 1   MEPNNVERNLQSLRRLYSLLVANARNEYIPEAYKLDDNTQFLLKRLLDFASHEHFVTQSN 60

Query: 61  NLATNSGIFYNFLHKDDKQTKALDEK----VAEWMEHNQTARKMVNPEIEYNAKRARASA 120
            LAT   +F   +    K +   D       A   + N +    V+  +E      R   
Sbjct: 61  LLATQLRVFPKTVLHGAKPSNVADSSETTPPAMLSQINGSHITRVSKPLEAKGTLQRDLR 120

Query: 121 SNVATNDLSNDIRNETLKQQKVQSRTDHSTLRTSFAESIKGHNLSSQLRSHLVGGQKVKP 180
            +   ++ S D+  E +     Q  T  S L  SF  S     + S  R+  V      P
Sbjct: 121 VDQIRSNPSKDVLTEEVVDAIEQIDTQLSAL--SFVSS----RVDSDERTRSVKSFVTPP 180

Query: 181 VVTNHCSEFVHGFRIPLRQDNDEAMKPPTVETRIFKQHKLVNPTTLIDKSGCSVGSKATV 240
             T  C +      +P  + N   +   +V +         +    +      +  +A  
Sbjct: 181 --TEECRQNSQA-SMPCLRSNYMTVSQKSVISGAEVTFPYNDQLVRVTSPPQPLPPRAVS 240

Query: 241 RFGLKLNQTQIQEKMIQHSYGRTIMRPTLLDH-------PSREVRKEQTHNKTHLATQQE 300
            F       +  +KM        IM+PTL+D         S E   +QT + T   ++ E
Sbjct: 241 GFKKPNQSNRASQKM-------PIMKPTLMDQETETFDDDSSETEADQTPSATGSESEDE 300

Query: 301 SKFT----NSESESTSSSSWTTQQTSESETTYDASSLSHQDGSPATGSEASSRYRSSSSS 360
              T    + E+ S+S S W TQ  +++E+  ++S     D S +           S+S 
Sbjct: 301 EVSTSQEYSGETGSSSGSEWETQAENDTESKSESSYPPQNDDSVS---------EVSTSP 360

Query: 361 ISTKAFKFNHGKKESERAIGRFKRLKNKLGLIFHHHHHHHHHHHHHHHHHNSHNFMWKQL 420
             T         K+    +GRFKR+KNK+G IFHHHHHHHHHHHHH     S    W +L
Sbjct: 361 PHTDRDTSREPGKQRRNVMGRFKRIKNKIGQIFHHHHHHHHHHHHHDKEKPS---AWNKL 420

Query: 421 RKIFHRTDNRKLTSKVERYGMLKKTAIRSVSRKNQVGKFEALAEGLRSHVWRSKPMKKKE 480
           +  FH     K  SK  +  M +   + +  +++Q G F AL EGL  H   SK  K + 
Sbjct: 421 QSKFHHKHQEK--SKERKRPMSESKGLTTHKQQHQGGHFHALVEGLVRHRKHSKKQKHQ- 480

Query: 481 LRGLNCGKKKGVKKLHWWKMFRRHR--GVKLPNKGRVKIG 502
                   K   KK  WWK+ ++ +  GVK+P +GRVK+G
Sbjct: 481 -------LKSDAKKTEWWKLLKKRQGGGVKIPKRGRVKLG 482

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022996027.11.1e-15664.73uncharacterized protein LOC111491355 isoform X2 [Cucurbita maxima][more]
XP_022996025.11.1e-15664.73uncharacterized protein LOC111491355 isoform X1 [Cucurbita maxima] >XP_022996026... [more]
XP_022958322.11.0e-15162.14uncharacterized protein LOC111459571 isoform X2 [Cucurbita moschata] >XP_0229583... [more]
XP_022958321.11.0e-15162.14uncharacterized protein LOC111459571 isoform X1 [Cucurbita moschata][more]
XP_038877121.12.0e-15061.26protein KOKOPELLI-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FFP24.2e-1526.92Protein KOKOPELLI OS=Arabidopsis thaliana OX=3702 GN=KPL PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1K5J45.2e-15764.73uncharacterized protein LOC111491355 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1K0S15.2e-15764.73uncharacterized protein LOC111491355 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1H2T75.0e-15262.14uncharacterized protein LOC111459571 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1H1S05.0e-15262.14uncharacterized protein LOC111459571 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1DNR37.5e-14059.51protein KOKOPELLI isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022084 PE=4... [more]
Match NameE-valueIdentityDescription
AT5G63720.13.0e-1626.92kokopelli [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 263..280
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 258..360
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 281..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032365.1Lag0032365.1mRNA