Lag0032362 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0032362
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	4a-hydroxytetrahydrobiopterin dehydratase
Location	chr11: 31258904 .. 31261113 (-)
RNA-Seq Expression	Lag0032362
Synteny	Lag0032362

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCAATCAGAGAAGGGCTGAAGTTTTACCTAGAGAAACCCATGGAGAGGAAGCCGCCCATGATCGTGGAATCGGATGCTTCAGAAGTGATTAAAGCCTTGAATTTCAAATGCGAAGAGTTATCGGACTCCAAATCTGTGATGGTCGAAATCGAGAGCCTCGCTTCGGATGTCGGGGTTATCTCGTTCTCAAAATGCCCTAGGGGTGTCAACTCCATCGCGCATACCCTTGCGCGAGCAGTGGCCGGCTTCATGCCGGAGATGGCTCCTGTGTCTCGTGATGCCGCTGTCTTAGTTAGTGATGAATTTGATGTTTTTTGTAATCCTTCTATGCTAGAAGGTGAGTTTTTTTTTGGAGGGAGGGTATTCTCCCAAATTTATTGTCCTCCCTGTTGGTGGAGGAGTTAGGTTGTACCTGTCCCAATTAGTTATTGAAGTTTTTCTTTCTCAAAAAAAAAAAAAAAAAAAAACAAAAAAACAAAAAAAAAATACCTCTAAACTATGAAGTTAGGTTTTGATTATACGAACATTGTTTTTATTAAAATTAAAATTTTTAATTTTTTTTACTAAAATATAATACTTTTCAAACTTATTATTTAAATATTGTACTTTTAAACTCGTGCATGAAGGTCGTGTCTTGAGGAGACGACCTCAACCACGTGTCAACATGTCATTTTACAAGTTTTAAAAATTATAATATTAATAAAAAAAAATATCCTCAATTATTTATTTTTTTTTAGAAGAAAATATATCCTCAATTATACTCTTGAACTTTTTAAAAGTTTCATTTTAGCTCACAAAGTTTGAAATTTGTTTCAAAATAACCTTTGAAGGATTTTTCATCAAAGTCATAGACCGAAAATGATGTGTTTGCATATGTATTATTGCTCATATGAATAAATTTGCACATACAAAACACATGTAAACATATCATTTTTCGTCCATAAATTTAATAGAAAATATTTTTAAAAATCATTTTGAAACAAACTTCAAACTCTAAGGGTTAATAAAATAAAACTTTGGAAAGTTTAGGGGTATAATAGAAACCAACCTCATAGTTCATGATTATTTTTAATAATTTATTTTATTTTATTTTTCTATTATTTAAAAAGAAAATAAGAAATAAAAACGCAGAAATAAAACCCATCCACAAGACTCTGAAAGAAAATTAAAGAAACACAAAACCCCACCAGAAAATAGCTTCCTAAGCGTGTTTCAATCTCATCCTTGAGGCTTCAAAGCATGGCCGCCACAACCTCATCCACCCGCTTCCCTCTCCTCCATCTCCCCCATCCCCCCGCCGCTTCTCTCTCCCGCCACCGCCGCCCGCCACCCTCCTCCGCCGCAAACCCCGCCCGGCCACCGTCAGAGCCACCGTGGCAGGCGACGGAGACTTCGGCGCCAGAGACCCCTTTCCGGCGGAAATCGAGAGCAACTTCGGCGAAAAAGTGATCGGATTCTCGAATACAGAGCACAAGATTCTGATCCCCAACGCCCGAGCCTTGGCTCTGTCTCAGCAACAGTGCCTTCCAGTTTCCTCTCTGCAGGCTCCAATGGCCGAAGATGAGGCCCAAAAGCTCTTGAAGAAGGTATGGAATTAGAATTAGAATTAGAATTTGATTTTGGTTTTGGTTTTATGAAATTTTGTGGGTTTTTGCAGGTGGTGGGATGGAGATTGGTGGATGATGGAAGAGGGCAATTGAAGCTTCAATGCTTGTGGAAATTGAGGGATTTCAAATGTAGTGTTGAGTTGATTAATAGAATCTGTGGAGTTGTTTCGGATGCTGGCCATTTTCCTGATATTCACCTGGAACAGCCTAATCAACTTCGAGCTGAGCTCTGGACTTCACCCATTGGTACTCTCTCTTTTAAACATTTTAATTTCATTCTCTAACTTCAACAACCAAATTCGCTTTACTTGTTTCAGGTAGGATCATAGATGTTTCATTTCACAGATTATACTTTTTTTAAAAAAGAAAATAATACGTATACATTTGTTGAGTCGTACTAATTTTCAAGTTCATTGTGAAGGAATATTAAAAATATGTTAAAAAAAGGTTAAATATTCCAAAATTTATAAGTTGAAGCTCAATAATTTTGGCTGTGTATCTGCAGGAGGTTTGAGTATGAATGATTTTATTGTAGCTGCAAAGATTGATGAGATAAAAACAATAGATCTTGCTCCCAGGAAAAGAGTTTGGGCATAG

mRNA sequence

ATGGCTGCAATCAGAGAAGGGCTGAAGTTTTACCTAGAGAAACCCATGGAGAGGAAGCCGCCCATGATCGTGGAATCGGATGCTTCAGAAGTGATTAAAGCCTTGAATTTCAAATGCGAAGAGTTATCGGACTCCAAATCTGTGATGGTCGAAATCGAGAGCCTCGCTTCGGATGTCGGGGTTATCTCGTTCTCAAAATGCCCTAGGGGTGTCAACTCCATCGCGCATACCCTTGCGCGAGCAGTGGCCGGCTTCATGCCGGAGATGGCTCCTGTGTCTCGTGATGCCGCTGTCTTAGCTTCAAAGCATGGCCGCCACAACCTCATCCACCCGCTTCCCTCTCCTCCATCTCCCCCATCCCCCCGCCGCTTCTCTCTCCCGCCACCGCCGCCCGCCACCCTCCTCCGCCGCAAACCCCGCCCGGCCACCGTCAGAGCCACCGTGGCAGGCGACGGAGACTTCGGCGCCAGAGACCCCTTTCCGGCGGAAATCGAGAGCAACTTCGGCGAAAAAGTGATCGGATTCTCGAATACAGAGCACAAGATTCTGATCCCCAACGCCCGAGCCTTGGCTCTGTCTCAGCAACAGTGCCTTCCAGTTTCCTCTCTGCAGGCTCCAATGGCCGAAGATGAGGCCCAAAAGCTCTTGAAGAAGGTGGTGGGATGGAGATTGGTGGATGATGGAAGAGGGCAATTGAAGCTTCAATGCTTGTGGAAATTGAGGGATTTCAAATGTAGTGTTGAGTTGATTAATAGAATCTGTGGAGTTGTTTCGGATGCTGGCCATTTTCCTGATATTCACCTGGAACAGCCTAATCAACTTCGAGCTGAGCTCTGGACTTCACCCATTGGAGGTTTGAGTATGAATGATTTTATTGTAGCTGCAAAGATTGATGAGATAAAAACAATAGATCTTGCTCCCAGGAAAAGAGTTTGGGCATAG

Coding sequence (CDS)

ATGGCTGCAATCAGAGAAGGGCTGAAGTTTTACCTAGAGAAACCCATGGAGAGGAAGCCGCCCATGATCGTGGAATCGGATGCTTCAGAAGTGATTAAAGCCTTGAATTTCAAATGCGAAGAGTTATCGGACTCCAAATCTGTGATGGTCGAAATCGAGAGCCTCGCTTCGGATGTCGGGGTTATCTCGTTCTCAAAATGCCCTAGGGGTGTCAACTCCATCGCGCATACCCTTGCGCGAGCAGTGGCCGGCTTCATGCCGGAGATGGCTCCTGTGTCTCGTGATGCCGCTGTCTTAGCTTCAAAGCATGGCCGCCACAACCTCATCCACCCGCTTCCCTCTCCTCCATCTCCCCCATCCCCCCGCCGCTTCTCTCTCCCGCCACCGCCGCCCGCCACCCTCCTCCGCCGCAAACCCCGCCCGGCCACCGTCAGAGCCACCGTGGCAGGCGACGGAGACTTCGGCGCCAGAGACCCCTTTCCGGCGGAAATCGAGAGCAACTTCGGCGAAAAAGTGATCGGATTCTCGAATACAGAGCACAAGATTCTGATCCCCAACGCCCGAGCCTTGGCTCTGTCTCAGCAACAGTGCCTTCCAGTTTCCTCTCTGCAGGCTCCAATGGCCGAAGATGAGGCCCAAAAGCTCTTGAAGAAGGTGGTGGGATGGAGATTGGTGGATGATGGAAGAGGGCAATTGAAGCTTCAATGCTTGTGGAAATTGAGGGATTTCAAATGTAGTGTTGAGTTGATTAATAGAATCTGTGGAGTTGTTTCGGATGCTGGCCATTTTCCTGATATTCACCTGGAACAGCCTAATCAACTTCGAGCTGAGCTCTGGACTTCACCCATTGGAGGTTTGAGTATGAATGATTTTATTGTAGCTGCAAAGATTGATGAGATAAAAACAATAGATCTTGCTCCCAGGAAAAGAGTTTGGGCATAG

Protein sequence

MAAIREGLKFYLEKPMERKPPMIVESDASEVIKALNFKCEELSDSKSVMVEIESLASDVGVISFSKCPRGVNSIAHTLARAVAGFMPEMAPVSRDAAVLASKHGRHNLIHPLPSPPSPPSPRRFSLPPPPPATLLRRKPRPATVRATVAGDGDFGARDPFPAEIESNFGEKVIGFSNTEHKILIPNARALALSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRLVDDGRGQLKLQCLWKLRDFKCSVELINRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMNDFIVAAKIDEIKTIDLAPRKRVWA

Homology

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BLAST of Lag0032362 vs. NCBI nr
Match: XP_022996033.1 (uncharacterized protein LOC111491359 [Cucurbita maxima] >XP_023532646.1 uncharacterized protein LOC111794746 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 335.1 bits (858), Expect = 6.4e-88
Identity = 169/203 (83.25%), Postives = 177/203 (87.19%), Query Frame = 0


Query: 111 PLPSPPSPPSPRRFSLPPPPPATLLRRKPRPATVRATVAGDGDFGARDPFPAEIESNFGE 170
           PL   P P S  RFSLPP P  TLLRRK RPAT+RA V GDGDFGARDPFPAEIESNFG+
Sbjct: 10  PLLRLPRPHS--RFSLPPSPSTTLLRRKLRPATIRAAVVGDGDFGARDPFPAEIESNFGD 69

Query: 171 KVIGFSNTEHKILIPNARALALSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRLVDDGRG 230
           KVIGFSNTEHKILIPN RALALSQQQC+P+SSLQAPM EDEAQKLLKKVVGWRLVD+GRG
Sbjct: 70  KVIGFSNTEHKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRLVDNGRG 129

Query: 231 QLKLQCLWKLRDFKCSVELINRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMND 290
           Q KL+CLWKLRDFKC VELINRI   V DA HFPDIHL+QPNQ+ AELWTS IGGLSMND
Sbjct: 130 QFKLRCLWKLRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGLSMND 189

Query: 291 FIVAAKIDEIKTIDLAPRKRVWA 314
           FIVAAKIDEIKTIDLA RKR WA
Sbjct: 190 FIVAAKIDEIKTIDLAARKRAWA 210

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BLAST of Lag0032362 vs. NCBI nr
Match: XP_022957679.1 (uncharacterized protein LOC111459142 [Cucurbita moschata] >KAG7035558.1 hypothetical protein SDJN02_02355 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 334.3 bits (856), Expect = 1.1e-87
Identity = 168/207 (81.16%), Postives = 178/207 (85.99%), Query Frame = 0


Query: 107 NLIHPLPSPPSPPSPRRFSLPPPPPATLLRRKPRPATVRATVAGDGDFGARDPFPAEIES 166
           N   PL   P P S  RFSLP  PP+T LRRK RPAT+RA V GDGDFGARDPFPAEIES
Sbjct: 6   NANFPLLRLPRPHS--RFSLPQTPPSTFLRRKLRPATIRAAVVGDGDFGARDPFPAEIES 65

Query: 167 NFGEKVIGFSNTEHKILIPNARALALSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRLVD 226
           NFG+KVIGFSNTEHKILIPN RALALSQQQC+P+SSLQAPM EDEAQKLLKKVVGWRLVD
Sbjct: 66  NFGDKVIGFSNTEHKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRLVD 125

Query: 227 DGRGQLKLQCLWKLRDFKCSVELINRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGL 286
           +GRGQ KL+CLWKLRDFKC VELINRI   V DA HFPDIHL+QPNQ+ AELWTS IGGL
Sbjct: 126 NGRGQFKLRCLWKLRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGL 185

Query: 287 SMNDFIVAAKIDEIKTIDLAPRKRVWA 314
           SMNDFI+AAKIDEIKTIDLA RKR WA
Sbjct: 186 SMNDFIIAAKIDEIKTIDLAARKRAWA 210

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BLAST of Lag0032362 vs. NCBI nr
Match: XP_038877111.1 (probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Benincasa hispida])

HSP 1 Score: 323.9 bits (829), Expect = 1.5e-84
Identity = 166/204 (81.37%), Postives = 176/204 (86.27%), Query Frame = 0


Query: 112 LPSPPSPPSPRRFSLPPPPPATLLRRKPRPATVRATVAGD--GDFGARDPFPAEIESNFG 171
           LP  P P   RRFS    PPA LLRR  R A+V  +V  D  GDFGARDPFPAEIESNFG
Sbjct: 11  LPLLPLPHHRRRFS---SPPAILLRRNRRSASVIVSVGADKLGDFGARDPFPAEIESNFG 70

Query: 172 EKVIGFSNTEHKILIPNARALALSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRLVDDGR 231
           EKV+GFS+TEHKILIPN RALALSQQ+C+PVSSLQAPMAEDEAQKLLKKVVGWRLVDDGR
Sbjct: 71  EKVVGFSDTEHKILIPNVRALALSQQECVPVSSLQAPMAEDEAQKLLKKVVGWRLVDDGR 130

Query: 232 GQLKLQCLWKLRDFKCSVELINRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMN 291
           G+ KLQCLWKLRDFKC VELINRI  VV + GHFPD+HLEQPNQ+RAELWTSPIGGLSMN
Sbjct: 131 GEFKLQCLWKLRDFKCCVELINRISKVVENVGHFPDLHLEQPNQVRAELWTSPIGGLSMN 190

Query: 292 DFIVAAKIDEIKTIDLAPRKRVWA 314
           DFIVAAKIDEIKT DLAPRKRVWA
Sbjct: 191 DFIVAAKIDEIKTSDLAPRKRVWA 211

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BLAST of Lag0032362 vs. NCBI nr
Match: XP_008436971.1 (PREDICTED: putative pterin-4-alpha-carbinolamine dehydratase [Cucumis melo] >KAA0055083.1 putative pterin-4-alpha-carbinolamine dehydratase [Cucumis melo var. makuwa])

HSP 1 Score: 315.1 bits (806), Expect = 6.9e-82
Identity = 163/216 (75.46%), Postives = 176/216 (81.48%), Query Frame = 0


Query: 99  LASKHGRHNLIH-PLPSPPSPPSPRRFSLPPPPPATLLRRKPRPATVRATVAGDGDFGAR 158
           +A+    H  +H PL   P P  PR  SL    P TLLRR  R  TV       GDFGAR
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHRPRFSSL----PTTLLRRSRRSVTVSVGTDILGDFGAR 60

Query: 159 DPFPAEIESNFGEKVIGFSNTEHKILIPNARALALSQQQCLPVSSLQAPMAEDEAQKLLK 218
           DPFPAEIESNFG+KVIG+SNTEHKILIPNARALALSQQ+C+PVSSLQAPMAEDEA+ LLK
Sbjct: 61  DPFPAEIESNFGDKVIGYSNTEHKILIPNARALALSQQECVPVSSLQAPMAEDEAKMLLK 120

Query: 219 KVVGWRLVDDGRGQLKLQCLWKLRDFKCSVELINRICGVVSDAGHFPDIHLEQPNQLRAE 278
           KVVGW+LVDDG G LKLQCLWKLRDFKCSVELINRIC VV + GHFPD+HLEQPNQ+RAE
Sbjct: 121 KVVGWKLVDDGSGGLKLQCLWKLRDFKCSVELINRICQVVENVGHFPDLHLEQPNQVRAE 180

Query: 279 LWTSPIGGLSMNDFIVAAKIDEIKTIDLAPRKRVWA 314
           LWTS IGGLSMND+IVAAKIDEIKT DLAPRKR WA
Sbjct: 181 LWTSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

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BLAST of Lag0032362 vs. NCBI nr
Match: XP_004143676.1 (probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Cucumis sativus] >KGN50323.1 hypothetical protein Csa_000223 [Cucumis sativus])

HSP 1 Score: 308.9 bits (790), Expect = 4.9e-80
Identity = 160/215 (74.42%), Postives = 175/215 (81.40%), Query Frame = 0


Query: 99  LASKHGRHNLIHPLPSPPSPPSPRRFSLPPPPPATLLRRKPRPATVRATVAGDGDFGARD 158
           +A+    H  +H LP  P P    R  L  P P TLLRR  R  TV       GDFGARD
Sbjct: 1   MATTSSTHLHLH-LPLLPLPQPHHRTFL--PLPTTLLRRNLRSLTVSVGTDILGDFGARD 60

Query: 159 PFPAEIESNFGEKVIGFSNTEHKILIPNARALALSQQQCLPVSSLQAPMAEDEAQKLLKK 218
           PFP EI SNFGEKVIGFS+TEHKILIPNARALALS+Q+C+PVSSLQAPMAEDEA+KLL+K
Sbjct: 61  PFPEEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRK 120

Query: 219 VVGWRLVDDGRGQLKLQCLWKLRDFKCSVELINRICGVVSDAGHFPDIHLEQPNQLRAEL 278
           VVGW+LVD GRG+LKLQCLWKLRDFKCSVELINRIC VV + GHFPD+HLEQPNQ+RAEL
Sbjct: 121 VVGWKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAEL 180

Query: 279 WTSPIGGLSMNDFIVAAKIDEIKTIDLAPRKRVWA 314
           WTS IGGLSMND+IVAAKIDEIKT DLAPRKR WA
Sbjct: 181 WTSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

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BLAST of Lag0032362 vs. ExPASy Swiss-Prot
Match: Q9LU63 (Probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATP1 PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 5.9e-60
Identity = 114/187 (60.96%), Postives = 142/187 (75.94%), Query Frame = 0


Query: 129 PPPATLLRRKPRPATVRATVAGD--GDFGARDPFPAEIESNFGEKVIGFSNTEHKILIPN 188
           PP +   R+  R    R+ +A D  GDFGARDP+P EI S FG+KV+G  +TEHKILIPN
Sbjct: 34  PPVSRNNRKLGRLTVTRSNLAQDFLGDFGARDPYPEEIASQFGDKVLGCQSTEHKILIPN 93

Query: 189 ARALALSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRLVDDGRGQLKLQCLWKLRDFKCS 248
           A  L+LSQ QC PVSS Q P++ D+A+ LL KV+GW +VD+  G LK++C+WK+RDF C 
Sbjct: 94  ASVLSLSQLQCSPVSSSQPPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCG 153

Query: 249 VELINRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMNDFIVAAKIDEIKTIDLA 308
           VELINRI  V   +GH+P +HLE P Q+RAEL+TS IGGLSMNDFI+AAKID+IKT DL+
Sbjct: 154 VELINRIHKVAEASGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLS 213

Query: 309 PRKRVWA 314
           PRKR WA
Sbjct: 214 PRKRAWA 220

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BLAST of Lag0032362 vs. ExPASy Swiss-Prot
Match: Q6QJ72 (Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PDL2 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.6e-20
Identity = 49/121 (40.50%), Postives = 74/121 (61.16%), Query Frame = 0


Query: 192 LSQQQCLPVSSLQ-APMAEDEAQKLLKKVVGWRLVDDGRGQLKLQCLWKLRDFKCSVELI 251
           LS ++C+P ++     M E  AQ LL+KV GW L +D    LKL   W+++ F   ++  
Sbjct: 66  LSTKKCVPCNAKDLRAMTEQSAQDLLQKVAGWDLAND-NDTLKLHRSWRVKSFTKGLDFF 125

Query: 252 NRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMNDFIVAAKIDEIKTIDLAPRKR 311
            R+  +    GH PD+HL   N ++ E+WT  IGGL+ NDFI+AAKI+E++  DL  +K+
Sbjct: 126 QRVADIAESEGHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKKK 185

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BLAST of Lag0032362 vs. ExPASy Swiss-Prot
Match: B0JQV0 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=MAE_39970 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 8.6e-11
Identity = 36/109 (33.03%), Postives = 59/109 (54.13%), Query Frame = 0


Query: 192 LSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRLVDDGRGQLKLQCLWKLRDFKCSVELIN 251
           L+QQ+C P  S  +P+  +E   L  K+  W L+ +  G  +LQ L+K  +F+ ++   N
Sbjct: 4   LAQQKCQPYQSGSSPITAEEITALQAKIPDWNLL-EYEGIPRLQKLYKFANFQGAIAFTN 63

Query: 252 RICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMNDFIVAAKIDEI 301
            +       GH P + L +  ++    WT  +GGL  NDFI+AA+ D+I
Sbjct: 64  AVGEAAEKEGHHPAL-LTEWGKVTVSWWTHDVGGLHQNDFIMAARTDDI 110

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BLAST of Lag0032362 vs. ExPASy Swiss-Prot
Match: A1TZA3 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8) OX=351348 GN=Maqu_0976 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.9e-10
Identity = 41/110 (37.27%), Postives = 58/110 (52.73%), Query Frame = 0


Query: 192 LSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRL-VDDGRGQLKLQCLWKLRDFKCSVELI 251
           L QQ C   S+    + + E Q LLK V  W+L V DG  Q  LQ ++  ++F  +    
Sbjct: 4   LGQQSCSACSADAPQVTDAEKQTLLKDVPEWQLIVQDGEEQ--LQRVFTFKNFVQAQAFT 63

Query: 252 NRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMNDFIVAAKIDEI 301
           N++  +  + GH P I LE   +     WT  IGGL  NDFI+AA+ DE+
Sbjct: 64  NKVADLAEEEGHHPAILLEW-GKATVRWWTHKIGGLHKNDFIMAARTDEL 110

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BLAST of Lag0032362 vs. ExPASy Swiss-Prot
Match: Q60BH8 (Putative pterin-4-alpha-carbinolamine dehydratase OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=MCA0497 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.2e-09
Identity = 38/112 (33.93%), Postives = 58/112 (51.79%), Query Frame = 0


Query: 189 ALALSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRLVDDGRGQLKLQCLWKLRDFKCSVE 248
           A +L+ +QC P      P+  +EA+KLL  V  W L D      KL+  ++  +F  +++
Sbjct: 3   ACSLTAKQCTPCQGGIPPLTAEEAEKLLVHVPRWELKD---AATKLKRTFRFENFMEALD 62

Query: 249 LINRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMNDFIVAAKIDEI 301
              ++  +    GH PDI +      R E  T  I GL  NDFI+AAK+DE+
Sbjct: 63  FARKVGELCEAEGHHPDIGIGW-GYCRVEFQTHKINGLHENDFIMAAKVDEL 110

[Close]

BLAST of Lag0032362 vs. ExPASy TrEMBL
Match: A0A6J1K9P8 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111491359 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 3.1e-88
Identity = 169/203 (83.25%), Postives = 177/203 (87.19%), Query Frame = 0


Query: 111 PLPSPPSPPSPRRFSLPPPPPATLLRRKPRPATVRATVAGDGDFGARDPFPAEIESNFGE 170
           PL   P P S  RFSLPP P  TLLRRK RPAT+RA V GDGDFGARDPFPAEIESNFG+
Sbjct: 10  PLLRLPRPHS--RFSLPPSPSTTLLRRKLRPATIRAAVVGDGDFGARDPFPAEIESNFGD 69

Query: 171 KVIGFSNTEHKILIPNARALALSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRLVDDGRG 230
           KVIGFSNTEHKILIPN RALALSQQQC+P+SSLQAPM EDEAQKLLKKVVGWRLVD+GRG
Sbjct: 70  KVIGFSNTEHKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRLVDNGRG 129

Query: 231 QLKLQCLWKLRDFKCSVELINRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMND 290
           Q KL+CLWKLRDFKC VELINRI   V DA HFPDIHL+QPNQ+ AELWTS IGGLSMND
Sbjct: 130 QFKLRCLWKLRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGLSMND 189

Query: 291 FIVAAKIDEIKTIDLAPRKRVWA 314
           FIVAAKIDEIKTIDLA RKR WA
Sbjct: 190 FIVAAKIDEIKTIDLAARKRAWA 210

[Close]

BLAST of Lag0032362 vs. ExPASy TrEMBL
Match: A0A6J1H2N5 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111459142 PE=3 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 5.3e-88
Identity = 168/207 (81.16%), Postives = 178/207 (85.99%), Query Frame = 0


Query: 107 NLIHPLPSPPSPPSPRRFSLPPPPPATLLRRKPRPATVRATVAGDGDFGARDPFPAEIES 166
           N   PL   P P S  RFSLP  PP+T LRRK RPAT+RA V GDGDFGARDPFPAEIES
Sbjct: 6   NANFPLLRLPRPHS--RFSLPQTPPSTFLRRKLRPATIRAAVVGDGDFGARDPFPAEIES 65

Query: 167 NFGEKVIGFSNTEHKILIPNARALALSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRLVD 226
           NFG+KVIGFSNTEHKILIPN RALALSQQQC+P+SSLQAPM EDEAQKLLKKVVGWRLVD
Sbjct: 66  NFGDKVIGFSNTEHKILIPNVRALALSQQQCVPISSLQAPMPEDEAQKLLKKVVGWRLVD 125

Query: 227 DGRGQLKLQCLWKLRDFKCSVELINRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGL 286
           +GRGQ KL+CLWKLRDFKC VELINRI   V DA HFPDIHL+QPNQ+ AELWTS IGGL
Sbjct: 126 NGRGQFKLRCLWKLRDFKCCVELINRISNAVDDANHFPDIHLDQPNQVTAELWTSAIGGL 185

Query: 287 SMNDFIVAAKIDEIKTIDLAPRKRVWA 314
           SMNDFI+AAKIDEIKTIDLA RKR WA
Sbjct: 186 SMNDFIIAAKIDEIKTIDLAARKRAWA 210

[Close]

BLAST of Lag0032362 vs. ExPASy TrEMBL
Match: A0A5A7UGL0 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00090 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 3.3e-82
Identity = 163/216 (75.46%), Postives = 176/216 (81.48%), Query Frame = 0


Query: 99  LASKHGRHNLIH-PLPSPPSPPSPRRFSLPPPPPATLLRRKPRPATVRATVAGDGDFGAR 158
           +A+    H  +H PL   P P  PR  SL    P TLLRR  R  TV       GDFGAR
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHRPRFSSL----PTTLLRRSRRSVTVSVGTDILGDFGAR 60

Query: 159 DPFPAEIESNFGEKVIGFSNTEHKILIPNARALALSQQQCLPVSSLQAPMAEDEAQKLLK 218
           DPFPAEIESNFG+KVIG+SNTEHKILIPNARALALSQQ+C+PVSSLQAPMAEDEA+ LLK
Sbjct: 61  DPFPAEIESNFGDKVIGYSNTEHKILIPNARALALSQQECVPVSSLQAPMAEDEAKMLLK 120

Query: 219 KVVGWRLVDDGRGQLKLQCLWKLRDFKCSVELINRICGVVSDAGHFPDIHLEQPNQLRAE 278
           KVVGW+LVDDG G LKLQCLWKLRDFKCSVELINRIC VV + GHFPD+HLEQPNQ+RAE
Sbjct: 121 KVVGWKLVDDGSGGLKLQCLWKLRDFKCSVELINRICQVVENVGHFPDLHLEQPNQVRAE 180

Query: 279 LWTSPIGGLSMNDFIVAAKIDEIKTIDLAPRKRVWA 314
           LWTS IGGLSMND+IVAAKIDEIKT DLAPRKR WA
Sbjct: 181 LWTSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

[Close]

BLAST of Lag0032362 vs. ExPASy TrEMBL
Match: A0A1S3ASJ9 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo OX=3656 GN=LOC103482540 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 3.3e-82
Identity = 163/216 (75.46%), Postives = 176/216 (81.48%), Query Frame = 0


Query: 99  LASKHGRHNLIH-PLPSPPSPPSPRRFSLPPPPPATLLRRKPRPATVRATVAGDGDFGAR 158
           +A+    H  +H PL   P P  PR  SL    P TLLRR  R  TV       GDFGAR
Sbjct: 1   MATTSSTHLHLHLPLLPLPQPHRPRFSSL----PTTLLRRSRRSVTVSVGTDILGDFGAR 60

Query: 159 DPFPAEIESNFGEKVIGFSNTEHKILIPNARALALSQQQCLPVSSLQAPMAEDEAQKLLK 218
           DPFPAEIESNFG+KVIG+SNTEHKILIPNARALALSQQ+C+PVSSLQAPMAEDEA+ LLK
Sbjct: 61  DPFPAEIESNFGDKVIGYSNTEHKILIPNARALALSQQECVPVSSLQAPMAEDEAKMLLK 120

Query: 219 KVVGWRLVDDGRGQLKLQCLWKLRDFKCSVELINRICGVVSDAGHFPDIHLEQPNQLRAE 278
           KVVGW+LVDDG G LKLQCLWKLRDFKCSVELINRIC VV + GHFPD+HLEQPNQ+RAE
Sbjct: 121 KVVGWKLVDDGSGGLKLQCLWKLRDFKCSVELINRICQVVENVGHFPDLHLEQPNQVRAE 180

Query: 279 LWTSPIGGLSMNDFIVAAKIDEIKTIDLAPRKRVWA 314
           LWTS IGGLSMND+IVAAKIDEIKT DLAPRKR WA
Sbjct: 181 LWTSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

[Close]

BLAST of Lag0032362 vs. ExPASy TrEMBL
Match: A0A0A0KL10 (4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis sativus OX=3659 GN=Csa_5G167150 PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 2.4e-80
Identity = 160/215 (74.42%), Postives = 175/215 (81.40%), Query Frame = 0


Query: 99  LASKHGRHNLIHPLPSPPSPPSPRRFSLPPPPPATLLRRKPRPATVRATVAGDGDFGARD 158
           +A+    H  +H LP  P P    R  L  P P TLLRR  R  TV       GDFGARD
Sbjct: 1   MATTSSTHLHLH-LPLLPLPQPHHRTFL--PLPTTLLRRNLRSLTVSVGTDILGDFGARD 60

Query: 159 PFPAEIESNFGEKVIGFSNTEHKILIPNARALALSQQQCLPVSSLQAPMAEDEAQKLLKK 218
           PFP EI SNFGEKVIGFS+TEHKILIPNARALALS+Q+C+PVSSLQAPMAEDEA+KLL+K
Sbjct: 61  PFPEEIASNFGEKVIGFSDTEHKILIPNARALALSEQECVPVSSLQAPMAEDEAKKLLRK 120

Query: 219 VVGWRLVDDGRGQLKLQCLWKLRDFKCSVELINRICGVVSDAGHFPDIHLEQPNQLRAEL 278
           VVGW+LVD GRG+LKLQCLWKLRDFKCSVELINRIC VV + GHFPD+HLEQPNQ+RAEL
Sbjct: 121 VVGWKLVDGGRGELKLQCLWKLRDFKCSVELINRICKVVENVGHFPDLHLEQPNQVRAEL 180

Query: 279 WTSPIGGLSMNDFIVAAKIDEIKTIDLAPRKRVWA 314
           WTS IGGLSMND+IVAAKIDEIKT DLAPRKR WA
Sbjct: 181 WTSSIGGLSMNDYIVAAKIDEIKTSDLAPRKRAWA 212

[Close]

BLAST of Lag0032362 vs. TAIR 10
Match: AT5G51110.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 232.6 bits (592), Expect = 4.2e-61
Identity = 114/187 (60.96%), Postives = 142/187 (75.94%), Query Frame = 0


Query: 129 PPPATLLRRKPRPATVRATVAGD--GDFGARDPFPAEIESNFGEKVIGFSNTEHKILIPN 188
           PP +   R+  R    R+ +A D  GDFGARDP+P EI S FG+KV+G  +TEHKILIPN
Sbjct: 34  PPVSRNNRKLGRLTVTRSNLAQDFLGDFGARDPYPEEIASQFGDKVLGCQSTEHKILIPN 93

Query: 189 ARALALSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRLVDDGRGQLKLQCLWKLRDFKCS 248
           A  L+LSQ QC PVSS Q P++ D+A+ LL KV+GW +VD+  G LK++C+WK+RDF C 
Sbjct: 94  ASVLSLSQLQCSPVSSSQPPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCG 153

Query: 249 VELINRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMNDFIVAAKIDEIKTIDLA 308
           VELINRI  V   +GH+P +HLE P Q+RAEL+TS IGGLSMNDFI+AAKID+IKT DL+
Sbjct: 154 VELINRIHKVAEASGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLS 213

Query: 309 PRKRVWA 314
           PRKR WA
Sbjct: 214 PRKRAWA 220

[Close]

BLAST of Lag0032362 vs. TAIR 10
Match: AT5G51110.2 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 232.3 bits (591), Expect = 5.5e-61
Identity = 111/182 (60.99%), Postives = 142/182 (78.02%), Query Frame = 0


Query: 132 ATLLRRKPRPATVRATVAGDGDFGARDPFPAEIESNFGEKVIGFSNTEHKILIPNARALA 191
           ++LL R+P  + ++  +   GDFGARDP+P EI S FG+KV+G  +TEHKILIPNA  L+
Sbjct: 15  SSLLLRQPSRSILKDFL---GDFGARDPYPEEIASQFGDKVLGCQSTEHKILIPNASVLS 74

Query: 192 LSQQQCLPVSSLQAPMAEDEAQKLLKKVVGWRLVDDGRGQLKLQCLWKLRDFKCSVELIN 251
           LSQ QC PVSS Q P++ D+A+ LL KV+GW +VD+  G LK++C+WK+RDF C VELIN
Sbjct: 75  LSQLQCSPVSSSQPPLSGDDARTLLHKVLGWSIVDNEAGGLKIRCMWKVRDFGCGVELIN 134

Query: 252 RICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMNDFIVAAKIDEIKTIDLAPRKRV 311
           RI  V   +GH+P +HLE P Q+RAEL+TS IGGLSMNDFI+AAKID+IKT DL+PRKR 
Sbjct: 135 RIHKVAEASGHYPSLHLESPTQVRAELFTSSIGGLSMNDFIMAAKIDDIKTSDLSPRKRA 193

Query: 312 WA 314
           WA
Sbjct: 195 WA 193

[Close]

BLAST of Lag0032362 vs. TAIR 10
Match: AT1G29810.1 (Transcriptional coactivator/pterin dehydratase )

HSP 1 Score: 101.7 bits (252), Expect = 1.1e-21
Identity = 49/121 (40.50%), Postives = 74/121 (61.16%), Query Frame = 0


Query: 192 LSQQQCLPVSSLQ-APMAEDEAQKLLKKVVGWRLVDDGRGQLKLQCLWKLRDFKCSVELI 251
           LS ++C+P ++     M E  AQ LL+KV GW L +D    LKL   W+++ F   ++  
Sbjct: 66  LSTKKCVPCNAKDLRAMTEQSAQDLLQKVAGWDLAND-NDTLKLHRSWRVKSFTKGLDFF 125

Query: 252 NRICGVVSDAGHFPDIHLEQPNQLRAELWTSPIGGLSMNDFIVAAKIDEIKTIDLAPRKR 311
            R+  +    GH PD+HL   N ++ E+WT  IGGL+ NDFI+AAKI+E++  DL  +K+
Sbjct: 126 QRVADIAESEGHHPDLHLVGWNNVKIEIWTHAIGGLTENDFILAAKINELQVEDLLRKKK 185

The following BLAST results are available for this feature:

BLAST of Lag0032362 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022996033.1	6.4e-88	83.25	uncharacterized protein LOC111491359 [Cucurbita maxima] >XP_023532646.1 uncharac...	[more]
XP_022957679.1	1.1e-87	81.16	uncharacterized protein LOC111459142 [Cucurbita moschata] >KAG7035558.1 hypothet...	[more]
XP_038877111.1	1.5e-84	81.37	probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Benincasa hisp...	[more]
XP_008436971.1	6.9e-82	75.46	PREDICTED: putative pterin-4-alpha-carbinolamine dehydratase [Cucumis melo] >KAA...	[more]
XP_004143676.1	4.9e-80	74.42	probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic [Cucumis sativu...	[more]

BLAST of Lag0032362 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LU63	5.9e-60	60.96	Probable pterin-4-alpha-carbinolamine dehydratase, chloroplastic OS=Arabidopsis ...	[more]
Q6QJ72	1.6e-20	40.50	Pterin-4-alpha-carbinolamine dehydratase 2, mitochondrial OS=Arabidopsis thalian...	[more]
B0JQV0	8.6e-11	33.03	Putative pterin-4-alpha-carbinolamine dehydratase OS=Microcystis aeruginosa (str...	[more]
A1TZA3	1.9e-10	37.27	Putative pterin-4-alpha-carbinolamine dehydratase OS=Marinobacter hydrocarbonocl...	[more]
Q60BH8	1.2e-09	33.93	Putative pterin-4-alpha-carbinolamine dehydratase OS=Methylococcus capsulatus (s...	[more]

BLAST of Lag0032362 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K9P8	3.1e-88	83.25	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1H2N5	5.3e-88	81.16	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A5A7UGL0	3.3e-82	75.46	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3ASJ9	3.3e-82	75.46	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis melo OX=3656 GN=LOC10348254...	[more]
A0A0A0KL10	2.4e-80	74.42	4a-hydroxytetrahydrobiopterin dehydratase OS=Cucumis sativus OX=3659 GN=Csa_5G16...	[more]

BLAST of Lag0032362 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51110.1	4.2e-61	60.96	Transcriptional coactivator/pterin dehydratase	[more]
AT5G51110.2	5.5e-61	60.99	Transcriptional coactivator/pterin dehydratase	[more]
AT1G29810.1	1.1e-21	40.50	Transcriptional coactivator/pterin dehydratase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001533	Pterin 4 alpha carbinolamine dehydratase	PFAM	PF01329	Pterin_4a	coord: 206..300 e-value: 2.1E-19 score: 69.4
IPR001533	Pterin 4 alpha carbinolamine dehydratase	PANTHER	PTHR12599	PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE	coord: 152..313
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 2..81 e-value: 2.8E-6 score: 27.2
IPR036428	Pterin 4 alpha carbinolamine dehydratase superfamily	GENE3D	3.30.1360.20	Transcriptional coactivator/pterin dehydratase	coord: 207..304 e-value: 5.2E-19 score: 69.9
IPR036428	Pterin 4 alpha carbinolamine dehydratase superfamily	SUPERFAMILY	55248	PCD-like	coord: 205..300
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 111..133
None	No IPR available	PANTHER	PTHR12599:SF8	PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE, CHLOROPLASTIC-RELATED	coord: 152..313
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 3..80 e-value: 8.10169E-9 score: 51.1608

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0032362.1	Lag0032362.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009737	response to abscisic acid
biological_process	GO:0009651	response to salt stress
biological_process	GO:0110102	ribulose bisphosphate carboxylase complex assembly
biological_process	GO:0006729	tetrahydrobiopterin biosynthetic process
cellular_component	GO:0071944	cell periphery
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008124	4-alpha-hydroxytetrahydrobiopterin dehydratase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity