Lag0032355 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032355
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPectinesterase
Locationchr11: 31201364 .. 31203985 (+)
RNA-Seq ExpressionLag0032355
SyntenyLag0032355
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCAAAAGCTCCATTTTTCAATCCTTTTCATTTCTCTGTCCATTTTATCATGGATCTCCCCATCGTTTTCAAGCAACTACAATGCCATAGATTTATGGTGCAGCCGAACCCCATATCCCGAAGAGTGCAAGCACTTCTTCAAGAACGGGAAATTCAACCCGAAAAAGCTGTCAGAGTTCAAGAAATCGGCGCTGAAAATCGCCATGGAAAGAGCTCTAACAGCTGAAACAGTGACAAAGCAACTGGGCCACAAATGCCGAAGCCAGAAAGAGAAAGCCGCATGGGCCGACTGTCTGGAGCTCTACCAGAACACAATCCTCCAACTCAACAGAACCTTCTCCGACCGATGCTCCGATTTCGACATCCAAACGTGGCTCAGCTCGGCGTTGACTAACCTCGAGACGTGCCGTGCTGGGTTCGTTGACCTGAACGTCAAGGACTACGACGCCGTTTTCCCCATTCTGGCGAGGAACAACGTGACGAAGCTCATCAGCAACAGTTTGGCGATGAATAACTGCACGGAGACGGAGACGGAGTACTCGGGGTACAGTGGCGGGTTTCCCAAGTGGGTTCGGGCGGGTGAGCGGCGGCTGCTGCAGGCGGCGGCGCCCAAGGCGAACTTGGTGGTGGCGCAGGATGGGTCGGGGAATTTTAGGACGGTTCAGGCGGCGGTGGACGCGGCGGCGAAGAGGAGTGGGAGTGGGAGGTTTGTTATACATATAAAGAGAGGGGTTTACAAGGAGAATGTTGTGATTGGGAATAAAGTGAAGAATTTGATGCTTGTTGGGGATGGATTGAGGTATACTATTATTACTGGAAGTAGAAGTGTTGGTGGTGGTTCCACAACTTTCAACTCTGCTACTGTTGGTGAGTTTCTTCTCAATCTCTTTCGTTTTTTTTTTTTACAATATGTGAGATGAGGGGTTCCGAACCAGACTTCGTAGTTATCGACCTAACTATATGTCAGTTGAGTTATGCTTTTGTTAGCTTTTTCGTTTTTTCTCTACCAACATTATTAAATTTTTATTTCAATTTTATGCCCTAAAATTTGTCTACCCAGTAATTATTTTAGTTATGTTTGAGGGTCATGAATATGTATTTGTTAAAGATATGGAATGGTTGATGATAAGCAAAATGGAATAAATGTCAAAGTATAGGAACGAAATAGACATCTAAAGTTTTTATTTATTTATTTTTATTTTTTGACAACCATAGACGTCTACGATTGAGGGACAATAATGAAAAAAAAATTGAATATTCAAAAACTAAAATAAACATGTTGAAAATTTAACAATTGTAATGAGATAATATGGAAAATTTAGGGACTACCATAAAATTTAGACCGAGTTGTTATTTTTAAATTGATCTAAGTGAAATTTATTTTTCACGGAAAATTAGTTTTGAAGAAATAAATATATGGATTTTTGTAGGTATACTAACTATCTTTTTTTTTTTTTTTTGAAACATACGTATACTAACTATCTTTATGATTATATAACAGTAGTGAAAATTACATAATATTTATTGAATTTTAACCCAAAACTTTTATAGGTGTGATGTTGAATATGTATGTGTATATATATATAGAACAAAACTTGGCTGTATATCGGTACGCAGGGATCTCCTTACGTACCCCATTAAAATTTTGACAACTAAAAAGGAGAAAGGGAGAAAATGAGAAAACTTTTTTATCTCTTAGCTGTCAAGATTTTAATGGAATATGTAGAGAGGTCCTTACTATGGATATCAGTCAACTCTTTTCAATATATATATTTTATTGAGTTAGCTTGTAAACAAAAAGAGTCTTAATTCTAAATTCATCAACCCTTTTCATATATAACAAGTTCCCATTTCACATTTCTTTCCTCACTTTACAACCCTTTATAAGTGCCTAATAACCCTTTGAAATTTTGTGACTTTGTTCAGCTGTGACTGGGGAAGGTTTCATTGCCCGTGGCATCACATTCCGCAACACCGCTGGACCCCAAAACCACCAAGCGGTAGCCCTCCGGTCGGGCTCCGATCTCTCTGTCTTCTACCAGTGCGGCTTCGAGGGATATCAAGACACTCTCTACGTCCATTCGCAAAGACAATTCTACAGAGAATGCTACATTTACGGTACCGTCGATTTCATCTTCGGCAACGCCGCCGTGGTCTTGCAAAACTGTATGATCTACGCAAGAAAACCCATCCCCGGCCAGCAATGCGTCGTCACCGCCCAAGGCCGGACCGACCCGAACCAGAACACCGGGATCTCGATCCACAACTCGAGAGTGATGGCCGCGGCCGACCTGAAGCCGGTGCTAAGCTCGGTGAGAACCTACTTGGGGAGGCCATGGAAGGAGTACTCTCGCACGGTTTACTTGCAAAGCTTCATGGATAGCTTGGTGAACCCTGCAGGGTGGTTGGAATGGAGTGGGAATTTTGCACTGAATACATTGTATTATGGAGAGTACAAGAATTTTGGGCCTGGTTCTTCTACTGCAAGGAGAGTGAAATGGAAGGGTTATAGAGTGATAACAAGTGTCACAGAAGCATCAAAGTTTACTGTTGGGAATTTTATTGCTGGGAAGTCATGGCTACCTGCCACTGGAGTGCCATTCAATGCTGGCCTCTGA

mRNA sequence

ATGGCTCAAAAGCTCCATTTTTCAATCCTTTTCATTTCTCTGTCCATTTTATCATGGATCTCCCCATCGTTTTCAAGCAACTACAATGCCATAGATTTATGGTGCAGCCGAACCCCATATCCCGAAGAGTGCAAGCACTTCTTCAAGAACGGGAAATTCAACCCGAAAAAGCTGTCAGAGTTCAAGAAATCGGCGCTGAAAATCGCCATGGAAAGAGCTCTAACAGCTGAAACAGTGACAAAGCAACTGGGCCACAAATGCCGAAGCCAGAAAGAGAAAGCCGCATGGGCCGACTGTCTGGAGCTCTACCAGAACACAATCCTCCAACTCAACAGAACCTTCTCCGACCGATGCTCCGATTTCGACATCCAAACGTGGCTCAGCTCGGCGTTGACTAACCTCGAGACGTGCCGTGCTGGGTTCGTTGACCTGAACGTCAAGGACTACGACGCCGTTTTCCCCATTCTGGCGAGGAACAACGTGACGAAGCTCATCAGCAACAGTTTGGCGATGAATAACTGCACGGAGACGGAGACGGAGTACTCGGGGTACAGTGGCGGGTTTCCCAAGTGGGTTCGGGCGGGTGAGCGGCGGCTGCTGCAGGCGGCGGCGCCCAAGGCGAACTTGGTGGTGGCGCAGGATGGGTCGGGGAATTTTAGGACGGTTCAGGCGGCGGTGGACGCGGCGGCGAAGAGGAGTGGGAGTGGGAGGTTTGTTATACATATAAAGAGAGGGGTTTACAAGGAGAATGTTGTGATTGGGAATAAAGTGAAGAATTTGATGCTTGTTGGGGATGGATTGAGGTATACTATTATTACTGGAAGTAGAAGTGTTGGTGGTGGTTCCACAACTTTCAACTCTGCTACTGTTGCTGTGACTGGGGAAGGTTTCATTGCCCGTGGCATCACATTCCGCAACACCGCTGGACCCCAAAACCACCAAGCGGTAGCCCTCCGGTCGGGCTCCGATCTCTCTGTCTTCTACCAGTGCGGCTTCGAGGGATATCAAGACACTCTCTACGTCCATTCGCAAAGACAATTCTACAGAGAATGCTACATTTACGGTACCGTCGATTTCATCTTCGGCAACGCCGCCGTGGTCTTGCAAAACTGTATGATCTACGCAAGAAAACCCATCCCCGGCCAGCAATGCGTCGTCACCGCCCAAGGCCGGACCGACCCGAACCAGAACACCGGGATCTCGATCCACAACTCGAGAGTGATGGCCGCGGCCGACCTGAAGCCGGTGCTAAGCTCGGTGAGAACCTACTTGGGGAGGCCATGGAAGGAGTACTCTCGCACGGTTTACTTGCAAAGCTTCATGGATAGCTTGGTGAACCCTGCAGGGTGGTTGGAATGGAGTGGGAATTTTGCACTGAATACATTGTATTATGGAGAGTACAAGAATTTTGGGCCTGGTTCTTCTACTGCAAGGAGAGTGAAATGGAAGGGTTATAGAGTGATAACAAGTGTCACAGAAGCATCAAAGTTTACTGTTGGGAATTTTATTGCTGGGAAGTCATGGCTACCTGCCACTGGAGTGCCATTCAATGCTGGCCTCTGA

Coding sequence (CDS)

ATGGCTCAAAAGCTCCATTTTTCAATCCTTTTCATTTCTCTGTCCATTTTATCATGGATCTCCCCATCGTTTTCAAGCAACTACAATGCCATAGATTTATGGTGCAGCCGAACCCCATATCCCGAAGAGTGCAAGCACTTCTTCAAGAACGGGAAATTCAACCCGAAAAAGCTGTCAGAGTTCAAGAAATCGGCGCTGAAAATCGCCATGGAAAGAGCTCTAACAGCTGAAACAGTGACAAAGCAACTGGGCCACAAATGCCGAAGCCAGAAAGAGAAAGCCGCATGGGCCGACTGTCTGGAGCTCTACCAGAACACAATCCTCCAACTCAACAGAACCTTCTCCGACCGATGCTCCGATTTCGACATCCAAACGTGGCTCAGCTCGGCGTTGACTAACCTCGAGACGTGCCGTGCTGGGTTCGTTGACCTGAACGTCAAGGACTACGACGCCGTTTTCCCCATTCTGGCGAGGAACAACGTGACGAAGCTCATCAGCAACAGTTTGGCGATGAATAACTGCACGGAGACGGAGACGGAGTACTCGGGGTACAGTGGCGGGTTTCCCAAGTGGGTTCGGGCGGGTGAGCGGCGGCTGCTGCAGGCGGCGGCGCCCAAGGCGAACTTGGTGGTGGCGCAGGATGGGTCGGGGAATTTTAGGACGGTTCAGGCGGCGGTGGACGCGGCGGCGAAGAGGAGTGGGAGTGGGAGGTTTGTTATACATATAAAGAGAGGGGTTTACAAGGAGAATGTTGTGATTGGGAATAAAGTGAAGAATTTGATGCTTGTTGGGGATGGATTGAGGTATACTATTATTACTGGAAGTAGAAGTGTTGGTGGTGGTTCCACAACTTTCAACTCTGCTACTGTTGCTGTGACTGGGGAAGGTTTCATTGCCCGTGGCATCACATTCCGCAACACCGCTGGACCCCAAAACCACCAAGCGGTAGCCCTCCGGTCGGGCTCCGATCTCTCTGTCTTCTACCAGTGCGGCTTCGAGGGATATCAAGACACTCTCTACGTCCATTCGCAAAGACAATTCTACAGAGAATGCTACATTTACGGTACCGTCGATTTCATCTTCGGCAACGCCGCCGTGGTCTTGCAAAACTGTATGATCTACGCAAGAAAACCCATCCCCGGCCAGCAATGCGTCGTCACCGCCCAAGGCCGGACCGACCCGAACCAGAACACCGGGATCTCGATCCACAACTCGAGAGTGATGGCCGCGGCCGACCTGAAGCCGGTGCTAAGCTCGGTGAGAACCTACTTGGGGAGGCCATGGAAGGAGTACTCTCGCACGGTTTACTTGCAAAGCTTCATGGATAGCTTGGTGAACCCTGCAGGGTGGTTGGAATGGAGTGGGAATTTTGCACTGAATACATTGTATTATGGAGAGTACAAGAATTTTGGGCCTGGTTCTTCTACTGCAAGGAGAGTGAAATGGAAGGGTTATAGAGTGATAACAAGTGTCACAGAAGCATCAAAGTTTACTGTTGGGAATTTTATTGCTGGGAAGTCATGGCTACCTGCCACTGGAGTGCCATTCAATGCTGGCCTCTGA

Protein sequence

MAQKLHFSILFISLSILSWISPSFSSNYNAIDLWCSRTPYPEECKHFFKNGKFNPKKLSEFKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSDRCSDFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNSLAMNNCTETETEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRFVIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTARRVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL
Homology
BLAST of Lag0032355 vs. NCBI nr
Match: XP_038875884.1 (pectinesterase 2-like [Benincasa hispida])

HSP 1 Score: 900.2 bits (2325), Expect = 8.3e-258
Identity = 450/523 (86.04%), Postives = 484/523 (92.54%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISPSFSS--NYNAIDLWCSRTPYPEECKHFFKNGKFNPKKL 60
           MAQKLHFSI+ ISL ILS IS  FS+  N NAIDLWCSR PYP+ CKHFF NG+F+PKKL
Sbjct: 1   MAQKLHFSIILISLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFF-NGQFDPKKL 60

Query: 61  SEFKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSD-R 120
           SEFKK+ALKIAM+RA+ AET+TK+LG KCR++KE+AAWADCLELYQ TIL LN+TFSD +
Sbjct: 61  SEFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDEK 120

Query: 121 CSDFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNSLAMNNCTET 180
           CSDFDIQTWLSSALT+LETCRAGF+DL +KDYD VFP LA NNVTKLISNSLAMNNC+E+
Sbjct: 121 CSDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLANNNVTKLISNSLAMNNCSES 180

Query: 181 ETEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGR 240
           +  Y   + GFPKWVR G+RRLLQAA PKA+LVVAQDGSGN+RTVQAAVDAA KR GSGR
Sbjct: 181 DVGYKS-NEGFPKWVRRGDRRLLQAAEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGSGR 240

Query: 241 FVIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGF 300
           FVIHIKRGVYKENVVI  KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGE F
Sbjct: 241 FVIHIKRGVYKENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERF 300

Query: 301 IARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTV 360
           IARGITFRNTAGP+NHQAVALRSGSDLSVF++CGFEGYQDTLY HSQRQFYRECYIYGTV
Sbjct: 301 IARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTV 360

Query: 361 DFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVL 420
           DFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAA DLKPV+
Sbjct: 361 DFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVV 420

Query: 421 SSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTA 480
            SVRTYLGRPWKEYSRTVYLQ+FMD+LVNPAGWLEWSGNFALNTLYYGE+KNFGPGSSTA
Sbjct: 421 GSVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSSTA 480

Query: 481 RRVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           +RVKWKGYRVITS TEASKFTVGNFIAG+SWLP TGVPF AGL
Sbjct: 481 KRVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 519

BLAST of Lag0032355 vs. NCBI nr
Match: XP_022996028.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 896.0 bits (2314), Expect = 1.6e-256
Identity = 450/522 (86.21%), Postives = 480/522 (91.95%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISPSFSSNYNAIDLWCSRTPYPEECKHFFKNGKFNPKKLSE 60
           MAQ LHF IL IS +IL  ISPSFS+  NA DLWCS+TPYP+ CK FFK  KFNPK L+E
Sbjct: 1   MAQNLHFPILVISFTILLSISPSFSAP-NAKDLWCSQTPYPDVCKSFFK-AKFNPKNLAE 60

Query: 61  FKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSDR-CS 120
           FKK+A KIAMERALTAET+TK+   KCR+ KEK AWADCLELYQ+TIL LNRTFSD  CS
Sbjct: 61  FKKTAFKIAMERALTAETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYCS 120

Query: 121 DFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILAR-NNVTKLISNSLAMNNCTETE 180
           DFDIQTWLSSALTNLETCRAGFVDLN+KDYD VFP L+R NNVTKLI NSLAMNNC+ETE
Sbjct: 121 DFDIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSETE 180

Query: 181 TEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRF 240
           T   GY GGFP WV+AGERRLLQAA PKA+LVVA DGSGN++TVQAAVDAAAKR GSGRF
Sbjct: 181 T---GYHGGFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300
           VIH+K+GVYKENVVIGNKVKNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGEGFI
Sbjct: 241 VIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300

Query: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVD 360
           ARGITFRNTAGP+NHQAVALRSGSDLSVFY+C FEGYQDTLYVHSQRQFYRECYIYGTVD
Sbjct: 301 ARGITFRNTAGPENHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECYIYGTVD 360

Query: 361 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLS 420
           FIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA ADL+PVLS
Sbjct: 361 FIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLS 420

Query: 421 SVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTAR 480
           SVRTYLGRPW+EYSRTVYLQ FMDSLVNPAGWLEWSG+FALNTLYYGE+KNFGPGSSTA+
Sbjct: 421 SVRTYLGRPWREYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAK 480

Query: 481 RVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           RVKWKGYRVITS  EASKFTVG+FIAGKSWLPATGVPF AGL
Sbjct: 481 RVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Lag0032355 vs. NCBI nr
Match: XP_023534348.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 896.0 bits (2314), Expect = 1.6e-256
Identity = 450/522 (86.21%), Postives = 480/522 (91.95%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISPSFSSNYNAIDLWCSRTPYPEECKHFFKNGKFNPKKLSE 60
           MAQ  HFSIL IS+SIL  ISPS S+  NA +LWCS+TPYP+ CK FF N KFNPK L+E
Sbjct: 1   MAQNRHFSILLISMSILLSISPSLSAP-NAKELWCSQTPYPDICKSFF-NAKFNPKNLAE 60

Query: 61  FKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSDR-CS 120
           FKK+A KIAMERALTAE++TK+   KCR+ KEK AWADCLELYQ+TILQLNRTFSD  CS
Sbjct: 61  FKKAAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCS 120

Query: 121 DFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILAR-NNVTKLISNSLAMNNCTETE 180
           DFDIQTWLSSALTNL+TCRAGFVDLN+KDYD VFP L R NNVTKLI NSLAMNNC+ETE
Sbjct: 121 DFDIQTWLSSALTNLDTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETE 180

Query: 181 TEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRF 240
           T   GY GGFP WV+AGERRLLQAA PK +LVVA DGSGN++TVQAAVDAAAKR GSGRF
Sbjct: 181 T---GYHGGFPTWVKAGERRLLQAAVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300
           VIH+K+GVYKENVVIGNKVKNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGEGFI
Sbjct: 241 VIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300

Query: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVD 360
           ARGITFRNTAGPQNHQAVALRSGSDLSVFY+CGFEGYQDTLYVHSQRQFYRECYIYGTVD
Sbjct: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVD 360

Query: 361 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLS 420
           FIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA ADL+PVLS
Sbjct: 361 FIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLS 420

Query: 421 SVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTAR 480
           SVRTYLGRPWKEYSRTVYLQ FMDSLVNPAGWLEWSG+FALNTLYYGE+KNFGPGSSTA+
Sbjct: 421 SVRTYLGRPWKEYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAK 480

Query: 481 RVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           RVKWKGYRVITS  EASKFTVG+FIAGKSWLPATGVPF AGL
Sbjct: 481 RVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Lag0032355 vs. NCBI nr
Match: XP_022957920.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 895.2 bits (2312), Expect = 2.7e-256
Identity = 450/522 (86.21%), Postives = 480/522 (91.95%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISPSFSSNYNAIDLWCSRTPYPEECKHFFKNGKFNPKKLSE 60
           MAQ  H SIL IS+SIL  ISPSFS+  NA DLWCS+TPYP+ CK FF N KFNPK L+E
Sbjct: 1   MAQNRHVSILVISMSILLAISPSFSAP-NAKDLWCSQTPYPDVCKSFF-NAKFNPKNLAE 60

Query: 61  FKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSDR-CS 120
           FKK+A KIAMERALTAE++TK+   KCR+ KEK AWADCLELYQ+TILQLNRTFSD  CS
Sbjct: 61  FKKTAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCS 120

Query: 121 DFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILAR-NNVTKLISNSLAMNNCTETE 180
           DFDIQTWLSSALTNLETCRAGFVDLN+KDYD VFP L R NNVTKLI NSLAMNNC+ETE
Sbjct: 121 DFDIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETE 180

Query: 181 TEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRF 240
           T   GY GGFP WV+AGERRLLQAA PKA+LVVA DGSGN++TVQAAVDAAAKR GSGRF
Sbjct: 181 T---GYHGGFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300
           VIH+K+GVYKENVVIGNKVKNLMLVGDGL+YTIITGSRSV GGSTTFNSATVAVTGEGFI
Sbjct: 241 VIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVRGGSTTFNSATVAVTGEGFI 300

Query: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVD 360
           ARGITFRNTAGPQNHQAVALRSGSDLSVFY+CGFEGYQDTLYVHSQRQFY+ECYIYGTVD
Sbjct: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVD 360

Query: 361 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLS 420
           FIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA ADL+PVLS
Sbjct: 361 FIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLS 420

Query: 421 SVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTAR 480
           SVRTYLGRPWKEYSRTVY+Q FMDSLVNPAGWLEWSG+FALNTLYYGE+KNFGPGSSTA+
Sbjct: 421 SVRTYLGRPWKEYSRTVYMQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAK 480

Query: 481 RVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           RVKWKGYRVITS  EASKFTVG+FIAGKSWLPATGVPF AGL
Sbjct: 481 RVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Lag0032355 vs. NCBI nr
Match: KAG6605649.1 (Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 884.4 bits (2284), Expect = 4.7e-253
Identity = 442/509 (86.84%), Postives = 471/509 (92.53%), Query Frame = 0

Query: 14  LSILSWISPSFSSNYNAIDLWCSRTPYPEECKHFFKNGKFNPKKLSEFKKSALKIAMERA 73
           +SIL  ISPSFS+  NA DLWCS+TPYP+ CK FF N KFNPK L+EFKK+A KIAMERA
Sbjct: 1   MSILLAISPSFSAP-NAKDLWCSQTPYPDVCKSFF-NAKFNPKNLAEFKKTAFKIAMERA 60

Query: 74  LTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSDR-CSDFDIQTWLSSALT 133
           LTAE++TK+   KCR+ KEK AWADCLELYQ+TILQLNRTFSD  CSDFDIQTWLSSALT
Sbjct: 61  LTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALT 120

Query: 134 NLETCRAGFVDLNVKDYDAVFPILAR-NNVTKLISNSLAMNNCTETETEYSGYSGGFPKW 193
           NLETCRAGFVDLN+KDYD VFP L R NNVTKLI NSLAMNNC+ETET   GY GGFP W
Sbjct: 121 NLETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETET---GYHGGFPTW 180

Query: 194 VRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRFVIHIKRGVYKENV 253
           V+AGERRLLQAA PKA+LVVA DGSGN++TVQAAVDAAAKR GSGRFVIH+K+GVYKENV
Sbjct: 181 VKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENV 240

Query: 254 VIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQ 313
           VIGNKVKNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQ
Sbjct: 241 VIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQ 300

Query: 314 NHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNC 373
           NHQAVALRSGSDLSVFY+CGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNC
Sbjct: 301 NHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNC 360

Query: 374 MIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLSSVRTYLGRPWKEY 433
           MIYARKP+ GQQC VTAQGR+DPNQNTGISIHNSRVMA ADL+PVLSSVRTYLGRPWKEY
Sbjct: 361 MIYARKPMSGQQCAVTAQGRSDPNQNTGISIHNSRVMATADLQPVLSSVRTYLGRPWKEY 420

Query: 434 SRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTARRVKWKGYRVITSV 493
           SRTVY+Q FMDSLVNPAGWLEWSG+FALNTLYYGE+KNFGPGSSTA+RVKWKGYRVITS 
Sbjct: 421 SRTVYMQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITSA 480

Query: 494 TEASKFTVGNFIAGKSWLPATGVPFNAGL 521
            EASKFTVG+FIAGKSWLPATGVPF AGL
Sbjct: 481 AEASKFTVGSFIAGKSWLPATGVPFTAGL 504

BLAST of Lag0032355 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 2.7e-182
Identity = 328/516 (63.57%), Postives = 403/516 (78.10%), Query Frame = 0

Query: 9   ILFISLSILSWISPSFSSNYNAIDLWCSRTPYPEECKHFF--KNGKFNPKKLSEFKKSAL 68
           ++ +SL + S    SF  +   +  WC +TP P+ C++F   K    + K+ ++F K +L
Sbjct: 6   LITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISL 65

Query: 69  KIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRT--FSDRCSDFDIQ 128
           ++A+ERA TA++ T  LG KCR+++EKAAW DC ELY+ T+L+LN+T   S  C+  D Q
Sbjct: 66  QLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQ 125

Query: 129 TWLSSALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNSLAMNNCTETETEYSGY 188
           TWLS+ALTNLETCRA   DL V +Y  V P+L+ NNVTKLISN+L++N     E     Y
Sbjct: 126 TWLSTALTNLETCRASLEDLGVPEY--VLPLLS-NNVTKLISNTLSLNKVPYNE---PSY 185

Query: 189 SGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRFVIHIKR 248
             GFP WV+ G+R+LLQ   P+AN+VVAQDGSGN +T+Q AV AAA R+G  R+VI+IK 
Sbjct: 186 KDGFPTWVKPGDRKLLQ-TTPRANIVVAQDGSGNVKTIQEAV-AAASRAGGSRYVIYIKA 245

Query: 249 GVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITF 308
           G Y EN+ +  K+KN+M VGDG+  TIITGS+SVGGG+TTF SATVAV G+ FIAR IT 
Sbjct: 246 GTYNENIEV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITI 305

Query: 309 RNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNA 368
           RNTAGP NHQAVALRSGSDLSVFY+C FEGYQDTLYVHSQRQFYREC IYGTVDFIFGNA
Sbjct: 306 RNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNA 365

Query: 369 AVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLSSVRTYL 428
           AVVLQNC I+ARKP P +   +TAQGRTDPNQ+TGI IHN RV AA+DLKPV SSV+T+L
Sbjct: 366 AVVLQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFL 425

Query: 429 GRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTARRVKWKG 488
           GRPWK+YSRTVY+++F+DSL+NPAGW+EWSG+FALNTLYY EY N GPGSSTA RVKW+G
Sbjct: 426 GRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRG 485

Query: 489 YRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           Y V+TS ++ S+FTVGNFIAG SWLPAT VPF +GL
Sbjct: 486 YHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of Lag0032355 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 598.6 bits (1542), Expect = 6.8e-170
Identity = 309/524 (58.97%), Postives = 395/524 (75.38%), Query Frame = 0

Query: 7   FSILFISLSILSWISPSFSSNYNAIDL--WCSRTPYPEECKHFFKNGKFNP--KKLSEFK 66
           F    I+  IL  +  S  S YN  D+  WCS+TP P+ C++F  +   N   K  SEF 
Sbjct: 4   FRAYIINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFL 63

Query: 67  KSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSD--RCSD 126
           K ++K+ ++RA+ A+T    LG KCR  +EKAAW DC++LY  T+ ++N T     +CS 
Sbjct: 64  KISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSK 123

Query: 127 FDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNSLAMN----NCTE 186
            D QTWLS+ALTNL+TCRAGF++L V   D V P+++ NNV+ L+ N+LA+N    N T 
Sbjct: 124 LDAQTWLSTALTNLDTCRAGFLELGVT--DIVLPLMS-NNVSNLLCNTLAINKVPFNYTP 183

Query: 187 TETEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSG 246
            E +      GFP WV+ G+R+LLQ++ PK N VVA+DGSGNF+T++ A+DAA   SGSG
Sbjct: 184 PEKD------GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA---SGSG 243

Query: 247 RFVIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEG 306
           RFVI++K+GVY EN+ I  + KN+ML GDG+  TIITGS+SVGGG+TTFNSATVA  G+G
Sbjct: 244 RFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDG 303

Query: 307 FIARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGT 366
           FIARGITFRNTAG  N QAVALRSGSDLSVFYQC FE YQDTLYVHS RQFYR+C +YGT
Sbjct: 304 FIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGT 363

Query: 367 VDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPV 426
           VDFIFGNAA VLQNC I+AR+P   +   +TAQGR+DPNQNTGI IHNSRV AA+DL+PV
Sbjct: 364 VDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPV 423

Query: 427 LSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSST 486
           L S +TYLGRPW++YSRTV++++ +DSL++P GWLEW GNFAL TL+Y E++N GPG+ST
Sbjct: 424 LGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGAST 483

Query: 487 ARRVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           + RV W G+RV+ S +EASKFTVG F+AG SW+P++ VPF +GL
Sbjct: 484 SGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Lag0032355 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 9.9e-161
Identity = 295/538 (54.83%), Postives = 380/538 (70.63%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISP-SFSSNYNAIDLWCSRTPYPEECKHFFK--NGKFNPKK 60
           M QKL    L + L +L  + P +  ++ N+ D WC +TPYP+ CK +FK  NG   P +
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPTQ 63

Query: 61  LSEFKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTF--- 120
           LSEF+   ++ AM+RA++A       G  C   K++A  ADC++LY +TI+QLNRT    
Sbjct: 64  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 123

Query: 121 ------SDRCSDFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNS 180
                 +  C+DFD QTWLS+ALTN ETCR G  DLNV D+  + PI++   ++ LISN 
Sbjct: 124 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDF--ITPIVSNTKISHLISNC 183

Query: 181 LAMNNCTET---ETEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAA 240
           LA+N    T   +   +    GFP W+   ++RLL+A   +ANLVVA+DGSG+F TVQAA
Sbjct: 184 LAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAA 243

Query: 241 VDAAAKRS-GSGRFVIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTT 300
           +D A +R   SGRFVI++KRG+Y+EN+ +     ++MLVGDG+R TIITG RSV GG TT
Sbjct: 244 IDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTT 303

Query: 301 FNSATVAVTGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQ 360
           +NSAT  + G  FIA+GITFRNTAGP   QAVALRS SDLS+FY+C  EGYQDTL VHSQ
Sbjct: 304 YNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQ 363

Query: 361 RQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHN 420
           RQFYRECYIYGTVDFIFGNAA V QNC+I  R+P+ GQ  V+TAQGR DP QNTGISIHN
Sbjct: 364 RQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHN 423

Query: 421 SRVMAAADLKPVLSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEW--SGNFALNTL 480
           SR++ A DLKPV+ +V+TY+GRPW ++SRTV LQ+++D++V+P GW  W     F L+TL
Sbjct: 424 SRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTL 483

Query: 481 YYGEYKNFGPGSSTARRVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           +Y EYKN GP SST  RV WKG+ V+   ++AS FTVG FIAG +WLP TG+PF +GL
Sbjct: 484 FYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Lag0032355 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 7.1e-159
Identity = 294/538 (54.65%), Postives = 376/538 (69.89%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISPSFS---SNYNAIDLWCSRTPYPEECKHFF-KNGKFN-P 60
           M Q + F  L + L +L  + P  +    N   ID WC +TPYP  CK +F K+  F  P
Sbjct: 5   MVQNISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLP 64

Query: 61  KKLSEFKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFS 120
            ++SEF+   ++ AM+RA++A          C   K++A  ADC+ LY +T++QLNRT  
Sbjct: 65  TQISEFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQ 124

Query: 121 D-------RCSDFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNS 180
                   RC+DFD QTWLS+ALTN ETCR G  DLNV D+    PI++   ++ LISN 
Sbjct: 125 GVSSKTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTT--PIVSNTKISHLISNC 184

Query: 181 LAMNNCTET---ETEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAA 240
           LA+N    T       +G S GFP WV   ERRLLQ  + +ANLVVA+DGSG+F+TVQAA
Sbjct: 185 LAVNGALLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAA 244

Query: 241 VDAAAKRS-GSGRFVIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTT 300
           +D A +R   SGRFVI++KRG+Y+EN+ +     N+MLVGDG+RYTIITG RSV GG TT
Sbjct: 245 IDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTT 304

Query: 301 FNSATVAVTGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQ 360
           ++SAT  + G  FIA+GI F+NTAGP   QAVALRS SDLS+FY+C  EGYQDTL VHSQ
Sbjct: 305 YSSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQ 364

Query: 361 RQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHN 420
           RQFYRECYIYGTVDFIFGNAAVV QNC+I  R P+ GQ  V+TAQGRTD  QNTGISIHN
Sbjct: 365 RQFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHN 424

Query: 421 SRVMAAADLKPVLSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWS--GNFALNTL 480
           S ++ A DLKPV+ SV+TY+GRPW  YSRTV L++++DS+V+P GW  W+    + L+TL
Sbjct: 425 SIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTL 484

Query: 481 YYGEYKNFGPGSSTARRVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           +Y EYKN GP SST  RV+WKG+ V++  ++AS F+VG FIAG +WLP +G+PF + L
Sbjct: 485 FYAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of Lag0032355 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 2.1e-155
Identity = 275/510 (53.92%), Postives = 366/510 (71.76%), Query Frame = 0

Query: 34  WCSRTPYPEECKHFFKNGKFN------PKKLSEFKKSALKIAMERALTAETVTKQLGHKC 93
           WC++TP+ E C ++F+    N      P+  SEF +  +K+A+++A+   + T + G  C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 94  RSQKEKAAWADCLELYQNTILQLNRTF--------SD-RCSDFDIQTWLSSALTNLETCR 153
            + + KAAW+DC+ L+QNT+ QLNRT         SD +C+DFD QTWLS+A TN+ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 154 AGFVDLNVKDYDAVFPILARNNVTKLISNSLAMNNC---TETETEYSGYSGGFPKWVRAG 213
           +G  DLNV D+  V P+++  N++ LI N LA+N         T  + +   FP WV   
Sbjct: 207 SGSEDLNVSDF--VMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRH 266

Query: 214 ERRLLQAAA---PKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRFVIHIKRGVYKENVV 273
           ERRLL +A+      +LVVAQD SG+FR++QAA++ AA+R    RFVI++K+GVY+EN+ 
Sbjct: 267 ERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENID 326

Query: 274 IGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQN 333
           +GN   N+MLVGDG R TIIT  RSV  G TT+NSAT    G+ F+A+ +TF NTAGP  
Sbjct: 327 VGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLR 386

Query: 334 HQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNCM 393
            QAVA+RS SDLSVFY+ G  G+QDTLY+HSQRQF+RECYI GT+DFIFGNAAVV QNCM
Sbjct: 387 GQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCM 446

Query: 394 IYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLSSVRTYLGRPWKEYS 453
           I  R+P+ GQ  ++TAQGR DP QNTGI+IH+SR++AA+DLKPV+ + +TYLGRPW+ YS
Sbjct: 447 ILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYS 506

Query: 454 RTVYLQSFMDSLVNPAGWLEW--SGNFALNTLYYGEYKNFGPGSSTARRVKWKGYRVITS 513
           R   +++++D+ ++P GW  W    NFALNT++YGEYKNFGPGSST  RV+WKG+  ITS
Sbjct: 507 RVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITS 566

Query: 514 VTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
              AS+FTVG+ IAG SWLPATGVPF +GL
Sbjct: 567 TAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Lag0032355 vs. ExPASy TrEMBL
Match: A0A6J1K9P3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 7.6e-257
Identity = 450/522 (86.21%), Postives = 480/522 (91.95%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISPSFSSNYNAIDLWCSRTPYPEECKHFFKNGKFNPKKLSE 60
           MAQ LHF IL IS +IL  ISPSFS+  NA DLWCS+TPYP+ CK FFK  KFNPK L+E
Sbjct: 1   MAQNLHFPILVISFTILLSISPSFSAP-NAKDLWCSQTPYPDVCKSFFK-AKFNPKNLAE 60

Query: 61  FKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSDR-CS 120
           FKK+A KIAMERALTAET+TK+   KCR+ KEK AWADCLELYQ+TIL LNRTFSD  CS
Sbjct: 61  FKKTAFKIAMERALTAETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYCS 120

Query: 121 DFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILAR-NNVTKLISNSLAMNNCTETE 180
           DFDIQTWLSSALTNLETCRAGFVDLN+KDYD VFP L+R NNVTKLI NSLAMNNC+ETE
Sbjct: 121 DFDIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSETE 180

Query: 181 TEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRF 240
           T   GY GGFP WV+AGERRLLQAA PKA+LVVA DGSGN++TVQAAVDAAAKR GSGRF
Sbjct: 181 T---GYHGGFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300
           VIH+K+GVYKENVVIGNKVKNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGEGFI
Sbjct: 241 VIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300

Query: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVD 360
           ARGITFRNTAGP+NHQAVALRSGSDLSVFY+C FEGYQDTLYVHSQRQFYRECYIYGTVD
Sbjct: 301 ARGITFRNTAGPENHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECYIYGTVD 360

Query: 361 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLS 420
           FIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA ADL+PVLS
Sbjct: 361 FIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLS 420

Query: 421 SVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTAR 480
           SVRTYLGRPW+EYSRTVYLQ FMDSLVNPAGWLEWSG+FALNTLYYGE+KNFGPGSSTA+
Sbjct: 421 SVRTYLGRPWREYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAK 480

Query: 481 RVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           RVKWKGYRVITS  EASKFTVG+FIAGKSWLPATGVPF AGL
Sbjct: 481 RVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Lag0032355 vs. ExPASy TrEMBL
Match: A0A6J1H1Z9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.3e-256
Identity = 450/522 (86.21%), Postives = 480/522 (91.95%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISPSFSSNYNAIDLWCSRTPYPEECKHFFKNGKFNPKKLSE 60
           MAQ  H SIL IS+SIL  ISPSFS+  NA DLWCS+TPYP+ CK FF N KFNPK L+E
Sbjct: 1   MAQNRHVSILVISMSILLAISPSFSAP-NAKDLWCSQTPYPDVCKSFF-NAKFNPKNLAE 60

Query: 61  FKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSDR-CS 120
           FKK+A KIAMERALTAE++TK+   KCR+ KEK AWADCLELYQ+TILQLNRTFSD  CS
Sbjct: 61  FKKTAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCS 120

Query: 121 DFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILAR-NNVTKLISNSLAMNNCTETE 180
           DFDIQTWLSSALTNLETCRAGFVDLN+KDYD VFP L R NNVTKLI NSLAMNNC+ETE
Sbjct: 121 DFDIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETE 180

Query: 181 TEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRF 240
           T   GY GGFP WV+AGERRLLQAA PKA+LVVA DGSGN++TVQAAVDAAAKR GSGRF
Sbjct: 181 T---GYHGGFPTWVKAGERRLLQAAVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300
           VIH+K+GVYKENVVIGNKVKNLMLVGDGL+YTIITGSRSV GGSTTFNSATVAVTGEGFI
Sbjct: 241 VIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVRGGSTTFNSATVAVTGEGFI 300

Query: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVD 360
           ARGITFRNTAGPQNHQAVALRSGSDLSVFY+CGFEGYQDTLYVHSQRQFY+ECYIYGTVD
Sbjct: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVD 360

Query: 361 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLS 420
           FIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA ADL+PVLS
Sbjct: 361 FIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVLS 420

Query: 421 SVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTAR 480
           SVRTYLGRPWKEYSRTVY+Q FMDSLVNPAGWLEWSG+FALNTLYYGE+KNFGPGSSTA+
Sbjct: 421 SVRTYLGRPWKEYSRTVYMQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAK 480

Query: 481 RVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           RVKWKGYRVITS  EASKFTVG+FIAGKSWLPATGVPF AGL
Sbjct: 481 RVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Lag0032355 vs. ExPASy TrEMBL
Match: A0A1S3ASJ4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 5.8e-249
Identity = 432/522 (82.76%), Postives = 470/522 (90.04%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISPSFS--SNYNAIDLWCSRTPYPEECKHFFKNGKFNPKKL 60
           MAQKLHFSIL  SL ILS  S  FS  +N  AIDLWCSRTPYP+ CK+FF +G+F+PK L
Sbjct: 1   MAQKLHFSILLFSLFILSSTSLPFSTKTNKKAIDLWCSRTPYPDVCKNFFNDGQFDPKNL 60

Query: 61  SEFKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSDRC 120
            + +K+ALKIAMERA+  ET+TK LG KCR++KE+AAWADCLELYQ TIL LN+TFSD C
Sbjct: 61  LDIQKTALKIAMERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDHC 120

Query: 121 SDFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNSLAMNNCTETE 180
           S+FDIQTWLSSALTNL+TCRAGFVDL +KDY  VFP LA NN+TKLISNSLAMNNC+E++
Sbjct: 121 SNFDIQTWLSSALTNLDTCRAGFVDLGIKDYGVVFPFLANNNITKLISNSLAMNNCSESD 180

Query: 181 TEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRF 240
            E +    GFPKWVR G+RRLLQAA PKA+LVVAQDGSGN++TVQAAVDAA KR GSGRF
Sbjct: 181 -EGNTSDEGFPKWVRGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRF 240

Query: 241 VIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFI 300
           VI IK+GVYKENVVI  KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGE FI
Sbjct: 241 VIRIKKGVYKENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFI 300

Query: 301 ARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVD 360
           ARGITFRNTAGPQNHQAVALRSG+DLSVF++CGFEGYQDTLY HSQRQFYRECYIYGT+D
Sbjct: 301 ARGITFRNTAGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTID 360

Query: 361 FIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLS 420
           FIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMAA DLKPV+ 
Sbjct: 361 FIFGNAAVVLQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIK 420

Query: 421 SVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTAR 480
           SVRTYLGRPWKEYSRTVYLQSFM+SLVNPAGWLEWSGNFALNTLYYGE+KN+GPGSSTA 
Sbjct: 421 SVRTYLGRPWKEYSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTAN 480

Query: 481 RVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           RVKWKGYRVITS  EA+KFTVGNFIAG SWLP TGVPF AGL
Sbjct: 481 RVKWKGYRVITSANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 519

BLAST of Lag0032355 vs. ExPASy TrEMBL
Match: A0A0A0KPG9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 3.0e-245
Identity = 426/523 (81.45%), Postives = 468/523 (89.48%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISPSFS--SNYNAIDLWCSRTPYPEECKHFFKNGKFNPKKL 60
           MAQKLHFSI+  S+ ILS  S  FS  +N  AI+LWCSRTPYP+ CKHFF NG+F+P+ L
Sbjct: 1   MAQKLHFSIILFSMFILSSSSLPFSTKTNNKAIELWCSRTPYPDVCKHFFNNGEFDPRNL 60

Query: 61  SEFKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSDR- 120
            + KK+ALKIAMERA+  ET+TK LG KCR++KE+AAWADCLELYQ TIL LN+TFSD+ 
Sbjct: 61  LDIKKAALKIAMERAMKTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDKN 120

Query: 121 CSDFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNSLAMNNCTET 180
           CS+FDIQTWLSSALTNL TCRAGFVDL +KDY  VFP L  NN+TKLISNSLAMNNC+E+
Sbjct: 121 CSNFDIQTWLSSALTNLHTCRAGFVDLGIKDYGVVFPFLENNNITKLISNSLAMNNCSES 180

Query: 181 ETEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGR 240
           + E +    GFPKW+  G+RRLLQAA PKA+LVVAQDGSGN++TVQAAVDAA KR GSGR
Sbjct: 181 D-EGNTSDEGFPKWLHGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGR 240

Query: 241 FVIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGF 300
           FVI +K+GVYKENVVI  KVKNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGE F
Sbjct: 241 FVIRVKKGVYKENVVI--KVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGERF 300

Query: 301 IARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTV 360
           IARGITFRNTAGPQNHQAVALRSG+DLSVF++CGFEGYQDTLYVHSQRQFYRECYIYGTV
Sbjct: 301 IARGITFRNTAGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYVHSQRQFYRECYIYGTV 360

Query: 361 DFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVL 420
           DFIFGN+AVVLQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMA  DLKPV+
Sbjct: 361 DFIFGNSAVVLQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMATDDLKPVI 420

Query: 421 SSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTA 480
            SVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGE+KN GPGSSTA
Sbjct: 421 KSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEFKNSGPGSSTA 480

Query: 481 RRVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
            RVKWKGYRVITS +EA+KFTVG+FIAG SWLP TGVPF AGL
Sbjct: 481 NRVKWKGYRVITSASEAAKFTVGSFIAGNSWLPGTGVPFTAGL 520

BLAST of Lag0032355 vs. ExPASy TrEMBL
Match: A0A5A7ULB7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00120 PE=3 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 5.0e-224
Identity = 386/451 (85.59%), Postives = 415/451 (92.02%), Query Frame = 0

Query: 70  MERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSDRCSDFDIQTWLSS 129
           MERA+  ET+TK LG KCR++KE+AAWADCLELYQ TIL LN+TFSD CS+FDIQTWLSS
Sbjct: 1   MERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDHCSNFDIQTWLSS 60

Query: 130 ALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNSLAMNNCTETETEYSGYSGGFP 189
           ALTNL+TCRAGFVDL +KDY  VFP LA NN+TKLISNSLAMNNC+E++ E +    GFP
Sbjct: 61  ALTNLDTCRAGFVDLGIKDYGVVFPFLANNNITKLISNSLAMNNCSESD-EGNTSDEGFP 120

Query: 190 KWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRFVIHIKRGVYKE 249
           KWVR G+RRLLQAA PKA+LVVAQDGSGN++TVQAAVDAA KR GSGRFVI IK+GVYKE
Sbjct: 121 KWVRGGDRRLLQAAEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRIKKGVYKE 180

Query: 250 NVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAG 309
           NVVI  KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGE FIARGITFRNTAG
Sbjct: 181 NVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNTAG 240

Query: 310 PQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQ 369
           PQNHQAVALRSG+DLSVF++CGFEGYQDTLY HSQRQFYRECYIYGT+DFIFGNAAVVLQ
Sbjct: 241 PQNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTIDFIFGNAAVVLQ 300

Query: 370 NCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLSSVRTYLGRPWK 429
           NCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMAA DLKPV+ SVRTYLGRPWK
Sbjct: 301 NCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIKSVRTYLGRPWK 360

Query: 430 EYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSSTARRVKWKGYRVIT 489
           EYSRTVYLQSFM+SLVNPAGWLEWSGNFALNTLYYGE+KN+GPGSSTA RVKWKGYRVIT
Sbjct: 361 EYSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTANRVKWKGYRVIT 420

Query: 490 SVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           S  EA+KFTVGNFIAG SWLP TGVPF AGL
Sbjct: 421 SANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 448

BLAST of Lag0032355 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 598.6 bits (1542), Expect = 4.8e-171
Identity = 309/524 (58.97%), Postives = 395/524 (75.38%), Query Frame = 0

Query: 7   FSILFISLSILSWISPSFSSNYNAIDL--WCSRTPYPEECKHFFKNGKFNP--KKLSEFK 66
           F    I+  IL  +  S  S YN  D+  WCS+TP P+ C++F  +   N   K  SEF 
Sbjct: 4   FRAYIINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFL 63

Query: 67  KSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFSD--RCSD 126
           K ++K+ ++RA+ A+T    LG KCR  +EKAAW DC++LY  T+ ++N T     +CS 
Sbjct: 64  KISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSK 123

Query: 127 FDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNSLAMN----NCTE 186
            D QTWLS+ALTNL+TCRAGF++L V   D V P+++ NNV+ L+ N+LA+N    N T 
Sbjct: 124 LDAQTWLSTALTNLDTCRAGFLELGVT--DIVLPLMS-NNVSNLLCNTLAINKVPFNYTP 183

Query: 187 TETEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAAVDAAAKRSGSG 246
            E +      GFP WV+ G+R+LLQ++ PK N VVA+DGSGNF+T++ A+DAA   SGSG
Sbjct: 184 PEKD------GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA---SGSG 243

Query: 247 RFVIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEG 306
           RFVI++K+GVY EN+ I  + KN+ML GDG+  TIITGS+SVGGG+TTFNSATVA  G+G
Sbjct: 244 RFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDG 303

Query: 307 FIARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGT 366
           FIARGITFRNTAG  N QAVALRSGSDLSVFYQC FE YQDTLYVHS RQFYR+C +YGT
Sbjct: 304 FIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGT 363

Query: 367 VDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPV 426
           VDFIFGNAA VLQNC I+AR+P   +   +TAQGR+DPNQNTGI IHNSRV AA+DL+PV
Sbjct: 364 VDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPV 423

Query: 427 LSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGPGSST 486
           L S +TYLGRPW++YSRTV++++ +DSL++P GWLEW GNFAL TL+Y E++N GPG+ST
Sbjct: 424 LGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGAST 483

Query: 487 ARRVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           + RV W G+RV+ S +EASKFTVG F+AG SW+P++ VPF +GL
Sbjct: 484 SGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of Lag0032355 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 568.2 bits (1463), Expect = 7.0e-162
Identity = 295/538 (54.83%), Postives = 380/538 (70.63%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISP-SFSSNYNAIDLWCSRTPYPEECKHFFK--NGKFNPKK 60
           M QKL    L + L +L  + P +  ++ N+ D WC +TPYP+ CK +FK  NG   P +
Sbjct: 4   MMQKLSILFLHLILLVLLCVHPLTTVADRNSTD-WCDKTPYPDPCKCYFKNHNGFQQPTQ 63

Query: 61  LSEFKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTF--- 120
           LSEF+   ++ AM+RA++A       G  C   K++A  ADC++LY +TI+QLNRT    
Sbjct: 64  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 123

Query: 121 ------SDRCSDFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNS 180
                 +  C+DFD QTWLS+ALTN ETCR G  DLNV D+  + PI++   ++ LISN 
Sbjct: 124 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDF--ITPIVSNTKISHLISNC 183

Query: 181 LAMNNCTET---ETEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAA 240
           LA+N    T   +   +    GFP W+   ++RLL+A   +ANLVVA+DGSG+F TVQAA
Sbjct: 184 LAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRAV--RANLVVAKDGSGHFNTVQAA 243

Query: 241 VDAAAKRS-GSGRFVIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTT 300
           +D A +R   SGRFVI++KRG+Y+EN+ +     ++MLVGDG+R TIITG RSV GG TT
Sbjct: 244 IDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTT 303

Query: 301 FNSATVAVTGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQ 360
           +NSAT  + G  FIA+GITFRNTAGP   QAVALRS SDLS+FY+C  EGYQDTL VHSQ
Sbjct: 304 YNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQ 363

Query: 361 RQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHN 420
           RQFYRECYIYGTVDFIFGNAA V QNC+I  R+P+ GQ  V+TAQGR DP QNTGISIHN
Sbjct: 364 RQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHN 423

Query: 421 SRVMAAADLKPVLSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEW--SGNFALNTL 480
           SR++ A DLKPV+ +V+TY+GRPW ++SRTV LQ+++D++V+P GW  W     F L+TL
Sbjct: 424 SRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTL 483

Query: 481 YYGEYKNFGPGSSTARRVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           +Y EYKN GP SST  RV WKG+ V+   ++AS FTVG FIAG +WLP TG+PF +GL
Sbjct: 484 FYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Lag0032355 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 562.0 bits (1447), Expect = 5.0e-160
Identity = 294/538 (54.65%), Postives = 376/538 (69.89%), Query Frame = 0

Query: 1   MAQKLHFSILFISLSILSWISPSFS---SNYNAIDLWCSRTPYPEECKHFF-KNGKFN-P 60
           M Q + F  L + L +L  + P  +    N   ID WC +TPYP  CK +F K+  F  P
Sbjct: 5   MVQNISFLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLP 64

Query: 61  KKLSEFKKSALKIAMERALTAETVTKQLGHKCRSQKEKAAWADCLELYQNTILQLNRTFS 120
            ++SEF+   ++ AM+RA++A          C   K++A  ADC+ LY +T++QLNRT  
Sbjct: 65  TQISEFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQ 124

Query: 121 D-------RCSDFDIQTWLSSALTNLETCRAGFVDLNVKDYDAVFPILARNNVTKLISNS 180
                   RC+DFD QTWLS+ALTN ETCR G  DLNV D+    PI++   ++ LISN 
Sbjct: 125 GVSSKTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTT--PIVSNTKISHLISNC 184

Query: 181 LAMNNCTET---ETEYSGYSGGFPKWVRAGERRLLQAAAPKANLVVAQDGSGNFRTVQAA 240
           LA+N    T       +G S GFP WV   ERRLLQ  + +ANLVVA+DGSG+F+TVQAA
Sbjct: 185 LAVNGALLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAA 244

Query: 241 VDAAAKRS-GSGRFVIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTT 300
           +D A +R   SGRFVI++KRG+Y+EN+ +     N+MLVGDG+RYTIITG RSV GG TT
Sbjct: 245 IDVAGRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTT 304

Query: 301 FNSATVAVTGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQ 360
           ++SAT  + G  FIA+GI F+NTAGP   QAVALRS SDLS+FY+C  EGYQDTL VHSQ
Sbjct: 305 YSSATAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQ 364

Query: 361 RQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHN 420
           RQFYRECYIYGTVDFIFGNAAVV QNC+I  R P+ GQ  V+TAQGRTD  QNTGISIHN
Sbjct: 365 RQFYRECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHN 424

Query: 421 SRVMAAADLKPVLSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWS--GNFALNTL 480
           S ++ A DLKPV+ SV+TY+GRPW  YSRTV L++++DS+V+P GW  W+    + L+TL
Sbjct: 425 SIIIPAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTL 484

Query: 481 YYGEYKNFGPGSSTARRVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           +Y EYKN GP SST  RV+WKG+ V++  ++AS F+VG FIAG +WLP +G+PF + L
Sbjct: 485 FYAEYKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of Lag0032355 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 550.4 bits (1417), Expect = 1.5e-156
Identity = 275/510 (53.92%), Postives = 366/510 (71.76%), Query Frame = 0

Query: 34  WCSRTPYPEECKHFFKNGKFN------PKKLSEFKKSALKIAMERALTAETVTKQLGHKC 93
           WC++TP+ E C ++F+    N      P+  SEF +  +K+A+++A+   + T + G  C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 94  RSQKEKAAWADCLELYQNTILQLNRTF--------SD-RCSDFDIQTWLSSALTNLETCR 153
            + + KAAW+DC+ L+QNT+ QLNRT         SD +C+DFD QTWLS+A TN+ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 154 AGFVDLNVKDYDAVFPILARNNVTKLISNSLAMNNC---TETETEYSGYSGGFPKWVRAG 213
           +G  DLNV D+  V P+++  N++ LI N LA+N         T  + +   FP WV   
Sbjct: 207 SGSEDLNVSDF--VMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRH 266

Query: 214 ERRLLQAAA---PKANLVVAQDGSGNFRTVQAAVDAAAKRSGSGRFVIHIKRGVYKENVV 273
           ERRLL +A+      +LVVAQD SG+FR++QAA++ AA+R    RFVI++K+GVY+EN+ 
Sbjct: 267 ERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENID 326

Query: 274 IGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQN 333
           +GN   N+MLVGDG R TIIT  RSV  G TT+NSAT    G+ F+A+ +TF NTAGP  
Sbjct: 327 VGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTAGPLR 386

Query: 334 HQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNCM 393
            QAVA+RS SDLSVFY+ G  G+QDTLY+HSQRQF+RECYI GT+DFIFGNAAVV QNCM
Sbjct: 387 GQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVFQNCM 446

Query: 394 IYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAADLKPVLSSVRTYLGRPWKEYS 453
           I  R+P+ GQ  ++TAQGR DP QNTGI+IH+SR++AA+DLKPV+ + +TYLGRPW+ YS
Sbjct: 447 ILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPWQAYS 506

Query: 454 RTVYLQSFMDSLVNPAGWLEW--SGNFALNTLYYGEYKNFGPGSSTARRVKWKGYRVITS 513
           R   +++++D+ ++P GW  W    NFALNT++YGEYKNFGPGSST  RV+WKG+  ITS
Sbjct: 507 RVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFHAITS 566

Query: 514 VTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
              AS+FTVG+ IAG SWLPATGVPF +GL
Sbjct: 567 TAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Lag0032355 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 460.3 bits (1183), Expect = 2.1e-129
Identity = 255/528 (48.30%), Postives = 331/528 (62.69%), Query Frame = 0

Query: 35  CSRTPYPEECKHFFKNGKFNPKKLSE------FKKSALKIAMERALTAETVTKQL-GHKC 94
           C +TPYP  C H   N     K L +      F    +   M++A+    +   L  H  
Sbjct: 38  CKQTPYPSVCDHHMSNSPL--KTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSSLKQHHS 97

Query: 95  RSQKEKAAWADCLELYQNTILQLN---RTFSDRCSDFDIQTWLSSALTNLETCRAGFVDL 154
             +   +A  DCLELY++TI QLN   R++    S  D QT LS+A+ N +TCR GF D 
Sbjct: 98  LHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDF 157

Query: 155 NV-KDYDAVFPILARNNVTKLISNSLAMNNC----------------TETETEYSGYSGG 214
            +   Y   FP+    N+TK ISNSLA+                   T+   + S   GG
Sbjct: 158 KLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGG 217

Query: 215 ------------FPKWVRAGERRLLQ--AAAPKANLVVAQDGSGNFRTVQAAVDAAAK-R 274
                       FP W    +R+LL+      KA+LVVA+DGSG++ ++Q AV+AAAK  
Sbjct: 218 SHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAKLP 277

Query: 275 SGSGRFVIHIKRGVYKENVVIGNKVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAV 334
             + R VI++K GVY+ENVVI   +KN+M++GDG+  TI+TG+R+V  G+TTF SAT AV
Sbjct: 278 RRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFAV 337

Query: 335 TGEGFIARGITFRNTAGPQNHQAVALRSGSDLSVFYQCGFEGYQDTLYVHSQRQFYRECY 394
           +G GFIA+GITF NTAGP+ HQAVALRS SD SVFY C F+GYQDTLY+HS RQF R C 
Sbjct: 338 SGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCN 397

Query: 395 IYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAAAD 454
           IYGTVDFIFG+A  +LQNC IYARKP+ GQ+  +TAQ R +P++ TG  I +S V  A++
Sbjct: 398 IYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE 457

Query: 455 LKPVLSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEYKNFGP 514
                    TYLGRPW+ +SRTV+++  + +LV+PAGWL WSG+FAL+TLYYGEY N G 
Sbjct: 458 ---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGA 517

Query: 515 GSSTARRVKWKGYRVITSVTEASKFTVGNFIAGKSWLPATGVPFNAGL 521
           G+S + RVKW GY VI +VTEA KFTV NF+ G  W+ ATGVP N GL
Sbjct: 518 GASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875884.18.3e-25886.04pectinesterase 2-like [Benincasa hispida][more]
XP_022996028.11.6e-25686.21pectinesterase-like [Cucurbita maxima][more]
XP_023534348.11.6e-25686.21pectinesterase-like [Cucurbita pepo subsp. pepo][more]
XP_022957920.12.7e-25686.21pectinesterase-like [Cucurbita moschata][more]
KAG6605649.14.7e-25386.84Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
O048872.7e-18263.57Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O221496.8e-17058.97Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN59.9e-16154.83Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN47.1e-15954.65Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3... [more]
Q9STY32.1e-15553.92Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1K9P37.6e-25786.21Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1[more]
A0A6J1H1Z91.3e-25686.21Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1[more]
A0A1S3ASJ45.8e-24982.76Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1[more]
A0A0A0KPG93.0e-24581.45Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1[more]
A0A5A7ULB75.0e-22485.59Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00120... [more]
Match NameE-valueIdentityDescription
AT2G45220.14.8e-17158.97Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51490.17.0e-16254.83Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51500.15.0e-16054.65Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G47400.11.5e-15653.92Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G23200.12.1e-12948.30Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 26..171
e-value: 7.4E-29
score: 111.9
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 30..171
e-value: 8.5E-26
score: 91.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 7..173
e-value: 1.0E-17
score: 62.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 202..520
e-value: 3.4E-127
score: 426.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 26..174
e-value: 2.0E-26
score: 94.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 29..174
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 208..505
e-value: 5.3E-145
score: 481.7
NoneNo IPR availablePANTHERPTHR31707:SF181PECTINESTERASEcoord: 6..519
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 6..519
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 33..173
e-value: 3.26974E-36
score: 129.866
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 353..362
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 234..253
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 203..519

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032355.1Lag0032355.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity