Lag0032306 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0032306
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DNA helicase
Location	chr11: 30134201 .. 30138856 (+)
RNA-Seq Expression	Lag0032306
Synteny	Lag0032306

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCGATGATGAAAATTCTGCCATCACCGACGAAGAAAGGGCTCGAATCTCCCGCAATTTCAGAGCTGCCAAAGCTCTACTTGCTCGTAAACGCCCTCGCCTTTTTCACTCCCATCAGCCCATTTCTCAGTGTTCTATTCATAACCATTCAGCTAACTCACTTCCTTACAGGTTCGTGTTCTTCGAGCTATTGTGTTTGCTCTGATTTGGATTAATTAGATAAAAATTTCATAGCATGCTGGTTTGTGCATTTTCCCTCTTTATGGAATCAAAGAAACACTAGTTCAAGATTTTGAGATTTCCCATTTGTTTTCTTGCCATTTTTTAATTCCAGAGTTGCGGATTTTAATGCAAACGAGGCCCCAGTTTGTGATAATGGTGCAAAAAGGGTTCCCTTGGCAGAGATTTCGGGAAACACTCCGTTATCGAAGTCATTGGCAATTGCAACTGACGCTAGTTCAAATGGGTTGGGGTTAGATTGTCTCAAGACTCCTGTTAAAAGTTCAGAATGTTCTAGTACAGAAAATTCTTTCTTAGCTCCAAGTATTCTGGATGATGATTTTGATGAGTCCACTTTAGAGGAAATTGATGCTCTGTGCGAGCAGAACTCATCTGCGAGAGCAGAAAGGCAAGGTTCACATGCCATCTTCCATGCGGCAAATCAAGATAATGATAGTTATAATGGTGATCTTAGTATAGATTTGGAGTCTGTAATTGGTAGCGAGAGTATAGAAACCAAAGACTCATCACGTTCATCGGATGCCTCGGAATTAAGAGCAGAGTGGATCAACTCTGACTCTGCAACTAAGAAGATCGGGACCATGCCTGAAGAGTATTCAAAATATTTGTTGTCTCTTAACGACAGACAACGAGAAGCAGCTTGCAGTGATATTTCAATCCCTTTAATGATTCTCGCTGGTCCGGGAAGTGGAAAGGTCTCATTCTTGTTCTCTTGTTTGTAAATTGAGATAAACAATATTGTTTCGGCTTTAATAAATGTTTTTGCTTTCAAGAATATTTTTTTAATTCGTGCTGAATAGTAAATCTCATCCAAGCTGTATTTTATAAGAATTGCTTAAGCAATACATGAGTACGTGTCTCCAATTCTAAATCTTCCTTAAGATCAGTCTGAGTTAAAGGTTTTTTTGCCCTCAAGAGCTGAAATTTCTGTTTTATGCAGACTTCCACAATGGTTGGTCGAGTTCTGATGCTGCTTAATGAGGTATTTCCACCTCTTGGCATTAATGTTTCTTTCTAATTTTGTTTTGTATTGTTTTTTTTTTTGTTTATTTAAAAATTTATTTTACTCTGATTTTTGTAACGACTCATTTTATTTCATTCCTACTCATAATTTTGGGACATTATTTGTTTAGTTACTCCTCCTTTTTGGTTGTGTAGGGTATAAGCCCCTCAAATATTTTGGCAATGACTTTCACTACTGCAGCTGCTTCTGAAATGAGAGATCGTGTTGGTGCAGTGGCCGGGAAGAAAATGGCAAAAGAACTTGTGATTAGCACATTTCATTCATTTTCATTGCAACTTTGTCGTTTACATGCAGAAAAGTACGCTCAAACTTTTCTTTTTACCTTGCCTAGATATGTGTTTCTAACTGGGTTTATGTTAGGTATCCGATCAGTATAACCTGTTAAGAAAGGAACAAATAGCCAAACATACACAGAAATGGATGGAAATATGACCCAACTCTACCCTATTGAGAGTTCTTCAATAGATAGAGGGGTTATTAGCCGTTATATTGATATTAAGAAGAAGAACAACAAGAACAACAAGAACAAGTAACCCCCAGCGATTCGAGAAGCTGGAATTCTCCCTCCTAGACCTGACTCAGTCTAAGCCAAAAATGATCCACACCCCAAAACGATCCCATCCCCTTAGTACAATAGGTGCTCACTCCCACGATCCCCTGTGGTCCCCCCTGCTCCCTCATCTAACGGTTTGTTGGCTAACTTTCCTATTCTGCCCTTCCTCTTGTATACCCGAATAATGGGAGGTCTAACAGTTTATCATTCTTTTTGTAGCCTTACTATGGATGGAGCACATTGATAGTTCAATTTCACTGCTTTATATATTTCAGTTGCCATATATACCAACAAGGAAATGATATGTATGGAAAAGGAAAACTTTACTGTATGCTATAAGTAGTAGGGACAACTAATAGAAGTGTAAGGAGATTAAACTGGAGTGAGAAAAATCTTTGATTAATATAGAATTATTGATAAAAGATATCTTCCTGCTCAGTCATAAGTCACAGGTAAATTTCTAAACTCATCCATGTAGGATCAGATCTGATTCTAATATTACAATCTTTCAAAATGAATATATGCTATATGCTATAGGACCCTTAGAACTCAAGGACACAGATGTACGACCTATGCTATTGTAAGGAAACCCATTACAATATATTGATATTCAGAGGAGAAAAGAAAAACAGTCCTAAGGCAATACAAAACTTCCAACAATTCAAGAGTGCTGGTTACTCTCCTCTATCTCTCACCACATTGAGCAGAAAATCAACATAAGAAACCCTCAAAGATAGAAACAGCACATGTATACTCCCTACCCCAACAACAAACTCTCCAGGCACACTCTCGGGACCACAGTGGTCCCCAATTGTACTCAGCACACACTCCCACATAACTAACTGTCCTCTACATTCTCTTTCCCTTTTCTACCCCTCCATTTATATACTTTCATTATAGAGGCGTAAGTTCGTAACAGCTATATGCTGTAAGATATGTAATCTGTATCTTGTTTATCTATATAGTCATTACTCATTAGTCTTGCTAAATCCCAAGTTGAAGCATTTCCTATCAAAGTGCACTTGCTCAATGGTCTTCCTTTTTATTAACCTTTGATCCTAGTTATTATCTTGCGGTTAGATTGTTATCTTATATTACAGGTTAGAGCGCACTCCGGATTTTTCAATATATGGACATGGGCAACAAAGGAGGGCAATAATTGAGGCTGTGCGTTTATTGGAAAATGAAAAAAGTAAACAAAAATTGGACTCCAACATACTTGGTGATGCTTCTAAGGATATTACTCCGCTGCATTTTAAGGATAAATCAAAGAAATGGCAAACATTTGTCCCTAAGGTTAGTAACTTTACTTTGTCAACTTTAGCTCAGGGTCTTGTGTGTTCTTTCCCTTCTTAGGGTTGTCCAAATGCTTTCTGAATAATTTAATTTCTTGGCAATTTAAATTCATATGATTGGATACTAATTGCTAGGCTTGAATAACTTGCAGAGTTTAAATGATGTGATGCACAATCTAGAAACAAGTAGTCTAAAGAAGGGGATGAATTGGTTGATTTACGATGTTTTACTTAATTTATGAAGTCATACAATTTAATTTTTGAATAAATTTAAATCAAAGTAGTGAGTACTCAAGATGTTTTGATTCTTTTAAGAGATTCTTAGTAGCAAATGAAAAGGCCTTCATTTTTGAGAGAATTGAGGAGGGGAGTTAATTTGGGAGAGGGGGACATGGAATGGAGACCTCAATCGACAAGTAATACTAATATTAGCTGGTTGGAGAGTTCTTTTGAGACGTTGAAGATTATTTATGACATTTGGAATGAGGGAGGTTCAAAGGCACTTGATCCTGATGGCTTTCTAATGAAGATATCTAGAGTCACCAACAATTGGTTTCCAATCCTATGAGGAGGTTATTTGTTGATAGGATAGATCAAACTTGACCTCCCAATCAGAAACCAGTGTTCTCAAAGGCATACTCCAACAAGACAAGTTTTCTAATCGCTTCAAGGCTAGAAGCAATAAATTTAGACATTGTTGCCTTAAGGCGAAGGTGATGAAAAGGTGTTACCTAAGTAAAGCAGGACACACTTTAATGTATAAATAAAACTTTATATAGTTTATAAACTTAAAACTGACCAGGCTAAGCTCAGGAGGTGGGCTTCTTGGGTGGGGTGTGATGTGGATTCATTTCCGTCCTCCTATTTAGGATTACCCTTAGAGCATAACCTTAGAAGTATATCATTTTGGGGTCCAGTCACCGATAAGGTTAGGAAAATACTAGCCTCTTGGAGAAAGAGTTTTTTCTCAAAAGGGGGAGACTTACTCTAAGTCAATCAGTTTTAAGTGGCATCCCTACCTACTATATGTCCCTTTTTAGAGTTCCTGAGAAGTATATACGGGACTCTTTATGGGAGGTAGTTGATGAAGGCAAAGGCTCGCACCTTGTGAATTGAATTGGAAGGTGGTCTCTAGTCCGATGGATTCAGGGGGGTTAGGTATTGGTAATGTTATGGATACGAACAAATCCTTGCTAGCTAAACGGCTTTGGCGCTTTCAGCTACAAGGTTATTGCTAGCAAGTATGAGCCTCACCTTTTTGAGTGGATTGTTGGTGGGGTTAAAGGCACTTCCAGAAATCCATGGGAAGAAATTTCAGCCCAGTTTCCTATTTATTCTCAGTTTATCCATAATGTGGTGGGGGATGAGAGGGATACTTATTTTGGGGAGGATAGGTGGATTGAGGATGCTCCCCTCTACTCTCTGTTTCCTCGTTTATATCAGTTGTCTTCTTTGAGGAATCATTCGGTGGCAACCATTCTTTCGTCTTCTGACAACCAGATTGCTCCTTCTCTGGGCTTTTGTAGACCCTTTGATAAATAG

mRNA sequence

ATGTTCGATGATGAAAATTCTGCCATCACCGACGAAGAAAGGGCTCGAATCTCCCGCAATTTCAGAGCTGCCAAAGCTCTACTTGCTCGTAAACGCCCTCGCCTTTTTCACTCCCATCAGCCCATTTCTCAGTGTTCTATTCATAACCATTCAGCTAACTCACTTCCTTACAGAGTTGCGGATTTTAATGCAAACGAGGCCCCAGTTTGTGATAATGGTGCAAAAAGGGTTCCCTTGGCAGAGATTTCGGGAAACACTCCGTTATCGAAGTCATTGGCAATTGCAACTGACGCTAGTTCAAATGGGTTGGGGTTAGATTGTCTCAAGACTCCTGTTAAAAGTTCAGAATGTTCTAGTACAGAAAATTCTTTCTTAGCTCCAAGTATTCTGGATGATGATTTTGATGAGTCCACTTTAGAGGAAATTGATGCTCTGTGCGAGCAGAACTCATCTGCGAGAGCAGAAAGGCAAGGTTCACATGCCATCTTCCATGCGGCAAATCAAGATAATGATAGTTATAATGGTGATCTTAGTATAGATTTGGAGTCTGTAATTGGTAGCGAGAGTATAGAAACCAAAGACTCATCACGTTCATCGGATGCCTCGGAATTAAGAGCAGAGTGGATCAACTCTGACTCTGCAACTAAGAAGATCGGGACCATGCCTGAAGAGTATTCAAAATATTTGTTGTCTCTTAACGACAGACAACGAGAAGCAGCTTGCAGTGATATTTCAATCCCTTTAATGATTCTCGCTGGTCCGGGAAGTGGAAAGACTTCCACAATGGTTGGTCGAGTTCTGATGCTGCTTAATGAGGGTATAAGCCCCTCAAATATTTTGGCAATGACTTTCACTACTGCAGCTGCTTCTGAAATGAGAGATCGTGTTGGTGCAGTGGCCGGGAAGAAAATGGCAAAAGAACTTGTGATTAGCACATTTCATTCATTTTCATTGCAACTTTGTCGTTTACATGCAGAAAAGTTAGAGCGCACTCCGGATTTTTCAATATATGGACATGGGCAACAAAGGAGGGCAATAATTGAGGCTGTGCGTTTATTGGAAAATGAAAAAAGTAAACAAAAATTGGACTCCAACATACTTGGTGATGCTTCTAAGGATATTACTCCGCTGCATTTTAAGGATAAATCAAAGAAATGGCAAACATTTGTCCCTAAGGATAGATCAAACTTGACCTCCCAATCAGAAACCAGTGTTCTCAAAGGCATACTCCAACAAGACAAGTTTTCTAATCGCTTCAAGGCTAGAAGCAATAAATTTAGACATTGTTGCCTTAAGGCGAAGGCTAAGCTCAGGAGGTGGGCTTCTTGGGTGGGGTGTGATGTGGATTCATTTCCGTCCTCCTATTTAGGATTACCCTTAGAGCATAACCTTAGAAGTATATCATTTTGGGGTCCAGTCACCGATAAGCTAAACGGCTTTGGCGCTTTCAGCTACAAGGTTATTGCTAGCAAGTATGAGCCTCACCTTTTTGAGTGGATTGTTGGTGGGGTTAAAGGCACTTCCAGAAATCCATGGGAAGAAATTTCAGCCCAGTTTCCTATTTATTCTCAGTTTATCCATAATGTGGTGGGGGATGAGAGGGATACTTATTTTGGGGAGGATAGGTGGATTGAGGATGCTCCCCTCTACTCTCTGTTTCCTCGTTTATATCAGTTGTCTTCTTTGAGGAATCATTCGGTGGCAACCATTCTTTCGTCTTCTGACAACCAGATTGCTCCTTCTCTGGGCTTTTGTAGACCCTTTGATAAATAG

Coding sequence (CDS)

ATGTTCGATGATGAAAATTCTGCCATCACCGACGAAGAAAGGGCTCGAATCTCCCGCAATTTCAGAGCTGCCAAAGCTCTACTTGCTCGTAAACGCCCTCGCCTTTTTCACTCCCATCAGCCCATTTCTCAGTGTTCTATTCATAACCATTCAGCTAACTCACTTCCTTACAGAGTTGCGGATTTTAATGCAAACGAGGCCCCAGTTTGTGATAATGGTGCAAAAAGGGTTCCCTTGGCAGAGATTTCGGGAAACACTCCGTTATCGAAGTCATTGGCAATTGCAACTGACGCTAGTTCAAATGGGTTGGGGTTAGATTGTCTCAAGACTCCTGTTAAAAGTTCAGAATGTTCTAGTACAGAAAATTCTTTCTTAGCTCCAAGTATTCTGGATGATGATTTTGATGAGTCCACTTTAGAGGAAATTGATGCTCTGTGCGAGCAGAACTCATCTGCGAGAGCAGAAAGGCAAGGTTCACATGCCATCTTCCATGCGGCAAATCAAGATAATGATAGTTATAATGGTGATCTTAGTATAGATTTGGAGTCTGTAATTGGTAGCGAGAGTATAGAAACCAAAGACTCATCACGTTCATCGGATGCCTCGGAATTAAGAGCAGAGTGGATCAACTCTGACTCTGCAACTAAGAAGATCGGGACCATGCCTGAAGAGTATTCAAAATATTTGTTGTCTCTTAACGACAGACAACGAGAAGCAGCTTGCAGTGATATTTCAATCCCTTTAATGATTCTCGCTGGTCCGGGAAGTGGAAAGACTTCCACAATGGTTGGTCGAGTTCTGATGCTGCTTAATGAGGGTATAAGCCCCTCAAATATTTTGGCAATGACTTTCACTACTGCAGCTGCTTCTGAAATGAGAGATCGTGTTGGTGCAGTGGCCGGGAAGAAAATGGCAAAAGAACTTGTGATTAGCACATTTCATTCATTTTCATTGCAACTTTGTCGTTTACATGCAGAAAAGTTAGAGCGCACTCCGGATTTTTCAATATATGGACATGGGCAACAAAGGAGGGCAATAATTGAGGCTGTGCGTTTATTGGAAAATGAAAAAAGTAAACAAAAATTGGACTCCAACATACTTGGTGATGCTTCTAAGGATATTACTCCGCTGCATTTTAAGGATAAATCAAAGAAATGGCAAACATTTGTCCCTAAGGATAGATCAAACTTGACCTCCCAATCAGAAACCAGTGTTCTCAAAGGCATACTCCAACAAGACAAGTTTTCTAATCGCTTCAAGGCTAGAAGCAATAAATTTAGACATTGTTGCCTTAAGGCGAAGGCTAAGCTCAGGAGGTGGGCTTCTTGGGTGGGGTGTGATGTGGATTCATTTCCGTCCTCCTATTTAGGATTACCCTTAGAGCATAACCTTAGAAGTATATCATTTTGGGGTCCAGTCACCGATAAGCTAAACGGCTTTGGCGCTTTCAGCTACAAGGTTATTGCTAGCAAGTATGAGCCTCACCTTTTTGAGTGGATTGTTGGTGGGGTTAAAGGCACTTCCAGAAATCCATGGGAAGAAATTTCAGCCCAGTTTCCTATTTATTCTCAGTTTATCCATAATGTGGTGGGGGATGAGAGGGATACTTATTTTGGGGAGGATAGGTGGATTGAGGATGCTCCCCTCTACTCTCTGTTTCCTCGTTTATATCAGTTGTCTTCTTTGAGGAATCATTCGGTGGCAACCATTCTTTCGTCTTCTGACAACCAGATTGCTCCTTCTCTGGGCTTTTGTAGACCCTTTGATAAATAG

Protein sequence

MFDDENSAITDEERARISRNFRAAKALLARKRPRLFHSHQPISQCSIHNHSANSLPYRVADFNANEAPVCDNGAKRVPLAEISGNTPLSKSLAIATDASSNGLGLDCLKTPVKSSECSSTENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIFHAANQDNDSYNGDLSIDLESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPEEYSKYLLSLNDRQREAACSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMTFTTAAASEMRDRVGAVAGKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRRAIIEAVRLLENEKSKQKLDSNILGDASKDITPLHFKDKSKKWQTFVPKDRSNLTSQSETSVLKGILQQDKFSNRFKARSNKFRHCCLKAKAKLRRWASWVGCDVDSFPSSYLGLPLEHNLRSISFWGPVTDKLNGFGAFSYKVIASKYEPHLFEWIVGGVKGTSRNPWEEISAQFPIYSQFIHNVVGDERDTYFGEDRWIEDAPLYSLFPRLYQLSSLRNHSVATILSSSDNQIAPSLGFCRPFDK

Homology

[Close]

BLAST of Lag0032306 vs. NCBI nr
Match: XP_022995240.1 (ATP-dependent DNA helicase SRS2-like protein At4g25120 [Cucurbita maxima])

HSP 1 Score: 633.6 bits (1633), Expect = 1.7e-177
Identity = 339/404 (83.91%), Postives = 356/404 (88.12%), Query Frame = 0


Query: 1   MFDDENSAITDEERARISRNFRAAKALLARKRPRLFHSHQPISQCSIHNHSANSLP--YR 60
           M +DENS IT+EER RISRNFRAAKALLARKRPRL HSHQPISQCS   HSA  LP   R
Sbjct: 1   MIEDENSGITEEERDRISRNFRAAKALLARKRPRLLHSHQPISQCSNRIHSATPLPNSSR 60

Query: 61  VADFNANEAPVCDNGAKRVPLAEISGNTPLSKSLAIATDASSNGLGLDCLKTPVKSSECS 120
           V+D NAN+APVCDNGAKRVPL+EISGNTPLSKS AIA DA+S GLGLDC KTP+KS ECS
Sbjct: 61  VSDLNANDAPVCDNGAKRVPLSEISGNTPLSKSFAIAVDATSKGLGLDCFKTPLKSYECS 120

Query: 121 STENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIFHAANQDNDSYNGDLS 180
           ST NSF APSILDDDFDESTLEEIDALCEQNSSARAER  SH+IFHA N DN SYN D S
Sbjct: 121 STRNSFSAPSILDDDFDESTLEEIDALCEQNSSARAERDDSHSIFHAENHDNGSYNDDPS 180

Query: 181 IDLESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPEEYSKYLLSLNDRQRE 240
           IDLE V GSES E K  S SSDA ELR+EWINSDSATKKIG MP EYSKYLLSLNDRQ+E
Sbjct: 181 IDLEFVNGSESSENKGLSCSSDALELRSEWINSDSATKKIGAMPAEYSKYLLSLNDRQQE 240

Query: 241 AACSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMTFTTAAASEMRDRVGA 300
           AACSDISIPLMILAGPGSGKTSTMVGRVLMLL+EGI P+NILAMTFTTAAASEMRDRVGA
Sbjct: 241 AACSDISIPLMILAGPGSGKTSTMVGRVLMLLSEGIGPANILAMTFTTAAASEMRDRVGA 300

Query: 301 VAGKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRRAIIEAVRLLENEKS 360
           V GKKMAKELVISTFHSFSLQLCRLHAEKLERT DFSIYGHGQQRRAIIEAVRLLENEKS
Sbjct: 301 VTGKKMAKELVISTFHSFSLQLCRLHAEKLERTSDFSIYGHGQQRRAIIEAVRLLENEKS 360

Query: 361 KQKLDSNILGDASKDITPLHFKDKSKKWQTFVPKDRSNLTSQSE 403
           KQK+DSNIL DASKD+ PLH KDKSKKWQTFVPK ++  T+  E
Sbjct: 361 KQKVDSNILDDASKDVAPLHLKDKSKKWQTFVPKAKACGTTSEE 404

[Close]

BLAST of Lag0032306 vs. NCBI nr
Match: KAG6605657.1 (ATP-dependent DNA helicase SRS2-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 630.6 bits (1625), Expect = 1.4e-176
Identity = 338/404 (83.66%), Postives = 357/404 (88.37%), Query Frame = 0


Query: 1   MFDDENSAITDEERARISRNFRAAKALLARKRPRLFHSHQPISQCSIHNHSAN--SLPYR 60
           M +DENS IT+EER RISRNFRAAKALLARKRPRL HSHQPISQCS   HSAN  SL  R
Sbjct: 127 MIEDENSGITEEERDRISRNFRAAKALLARKRPRLLHSHQPISQCSNRIHSANPLSLSSR 186

Query: 61  VADFNANEAPVCDNGAKRVPLAEISGNTPLSKSLAIATDASSNGLGLDCLKTPVKSSECS 120
           V+D +AN+APVCDNGAKRVPL+EISGNTP+SKS AIA DA+S  LGLDC KTP+KSSECS
Sbjct: 187 VSDLDANDAPVCDNGAKRVPLSEISGNTPVSKSFAIAIDATSKELGLDCFKTPLKSSECS 246

Query: 121 STENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIFHAANQDNDSYNGDLS 180
           ST NSF APSILDDDFDESTLEEI ALCEQNSSARAER GSH+IFHA N DN SYN D S
Sbjct: 247 STRNSFSAPSILDDDFDESTLEEIAALCEQNSSARAERDGSHSIFHAENHDNGSYNDDPS 306

Query: 181 IDLESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPEEYSKYLLSLNDRQRE 240
           IDLESV GSE  E K  S SSDA ELRAEWINSDSATKKIG MP EY+KYLLSLNDRQ+E
Sbjct: 307 IDLESVNGSERSENKGLSCSSDAFELRAEWINSDSATKKIGAMPAEYAKYLLSLNDRQQE 366

Query: 241 AACSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMTFTTAAASEMRDRVGA 300
           AACSDISIPLMILAGPGSGKTSTMVGRVLMLL+EGI P+NILAMTFTTAAASEMRDRVGA
Sbjct: 367 AACSDISIPLMILAGPGSGKTSTMVGRVLMLLSEGIGPANILAMTFTTAAASEMRDRVGA 426

Query: 301 VAGKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRRAIIEAVRLLENEKS 360
           V GKKMAKELVISTFHSFSLQLCRLHAEKLERT DFSIYGHGQQRRAIIEAVRLLENEKS
Sbjct: 427 VTGKKMAKELVISTFHSFSLQLCRLHAEKLERTSDFSIYGHGQQRRAIIEAVRLLENEKS 486

Query: 361 KQKLDSNILGDASKDITPLHFKDKSKKWQTFVPKDRSNLTSQSE 403
           KQK+DSNIL DASKD+ PLH KDKSKKWQTFVPK ++  T+  E
Sbjct: 487 KQKVDSNILDDASKDVAPLHLKDKSKKWQTFVPKAKACGTTSEE 530

[Close]

BLAST of Lag0032306 vs. NCBI nr
Match: XP_023532520.1 (ATP-dependent DNA helicase SRS2-like protein At4g25120 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 629.0 bits (1621), Expect = 4.1e-176
Identity = 338/404 (83.66%), Postives = 356/404 (88.12%), Query Frame = 0


Query: 1   MFDDENSAITDEERARISRNFRAAKALLARKRPRLFHSHQPISQCSIHNHSANSLPY--R 60
           M +DENS IT+EER RISRNFRAAKALLARKRPRL HSHQPISQ S   HSAN LP   R
Sbjct: 1   MIEDENSGITEEERDRISRNFRAAKALLARKRPRLLHSHQPISQRSNRIHSANLLPISSR 60

Query: 61  VADFNANEAPVCDNGAKRVPLAEISGNTPLSKSLAIATDASSNGLGLDCLKTPVKSSECS 120
           V+D NA +APVCDNGAKRVPL+EISGNTPLSKS AIA DA+S  LGLDC KTP+KSSECS
Sbjct: 61  VSDLNATDAPVCDNGAKRVPLSEISGNTPLSKSFAIAIDATSKELGLDCFKTPLKSSECS 120

Query: 121 STENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIFHAANQDNDSYNGDLS 180
           ST NSF APSILDDDFDESTLEEIDALCEQNSSARAER  SH+IFHA NQDN SYN D S
Sbjct: 121 STRNSFSAPSILDDDFDESTLEEIDALCEQNSSARAERDCSHSIFHAGNQDNGSYNDDPS 180

Query: 181 IDLESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPEEYSKYLLSLNDRQRE 240
           +DLESV GSES E K    SSDA ELRAEWINSDSATKKIG MP EY+KYLLSLNDRQ+E
Sbjct: 181 VDLESVNGSESSENKGLLCSSDALELRAEWINSDSATKKIGAMPAEYAKYLLSLNDRQQE 240

Query: 241 AACSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMTFTTAAASEMRDRVGA 300
           AACSDISIPLMILAGPGSGKTSTMVGRVLMLL+EGI P+NILAMTFTTAAASEMRDRVGA
Sbjct: 241 AACSDISIPLMILAGPGSGKTSTMVGRVLMLLSEGIGPANILAMTFTTAAASEMRDRVGA 300

Query: 301 VAGKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRRAIIEAVRLLENEKS 360
           V GKKMAKELVISTFHSFSLQLCRLHAEKLERT DFSIYGHGQQRRAIIEAVRLLENEKS
Sbjct: 301 VTGKKMAKELVISTFHSFSLQLCRLHAEKLERTSDFSIYGHGQQRRAIIEAVRLLENEKS 360

Query: 361 KQKLDSNILGDASKDITPLHFKDKSKKWQTFVPKDRSNLTSQSE 403
           KQK+DSNIL DASKD+ PLH KDKSKKWQTFVPK ++  T+  E
Sbjct: 361 KQKVDSNILDDASKDVAPLHLKDKSKKWQTFVPKAKACGTTSEE 404

[Close]

BLAST of Lag0032306 vs. NCBI nr
Match: KAG7035567.1 (ATP-dependent DNA helicase SRS2-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 624.8 bits (1610), Expect = 7.7e-175
Identity = 337/404 (83.42%), Postives = 357/404 (88.37%), Query Frame = 0


Query: 1   MFDDENSAITDEERARISRNFRAAKALLARKRPRLFHSHQPISQCSIHNHSAN--SLPYR 60
           M +DENS IT+EER RISRNFRAAKALLARKRPRL HSHQPISQCS   HSAN  SL  R
Sbjct: 1   MIEDENSGITEEERDRISRNFRAAKALLARKRPRLLHSHQPISQCSNRIHSANPLSLSSR 60

Query: 61  VADFNANEAPVCDNGAKRVPLAEISGNTPLSKSLAIATDASSNGLGLDCLKTPVKSSECS 120
           V+D +AN+APVCDNGAKRVPL+EISGNTP+SKS AIA DA+S  LGLDC KTP+KSSECS
Sbjct: 61  VSDLDANDAPVCDNGAKRVPLSEISGNTPVSKSFAIAIDATSKELGLDCFKTPLKSSECS 120

Query: 121 STENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIFHAANQDNDSYNGDLS 180
           ST NSF APSILDDDFDESTLEEI ALCEQNSSARAER GSH+IFHA N DN SYN D S
Sbjct: 121 STRNSFSAPSILDDDFDESTLEEIAALCEQNSSARAERDGSHSIFHAENHDNGSYNDDPS 180

Query: 181 IDLESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPEEYSKYLLSLNDRQRE 240
           IDLESV GSE+   K  S SSDA ELRAEWINSDSATKKIG MP EY+KYLLSLNDRQ+E
Sbjct: 181 IDLESVNGSEN---KGLSCSSDALELRAEWINSDSATKKIGAMPAEYAKYLLSLNDRQQE 240

Query: 241 AACSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMTFTTAAASEMRDRVGA 300
           AACSDISIPLMILAGPGSGKTSTMVGRVLMLL+EGI P+NILAMTFTTAAASEMRDRVGA
Sbjct: 241 AACSDISIPLMILAGPGSGKTSTMVGRVLMLLSEGIGPANILAMTFTTAAASEMRDRVGA 300

Query: 301 VAGKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRRAIIEAVRLLENEKS 360
           V GKKMAKELVISTFHSFSLQLCRLHAEKLERT DFSIYGHGQQRRAIIEAVRLLENEKS
Sbjct: 301 VTGKKMAKELVISTFHSFSLQLCRLHAEKLERTSDFSIYGHGQQRRAIIEAVRLLENEKS 360

Query: 361 KQKLDSNILGDASKDITPLHFKDKSKKWQTFVPKDRSNLTSQSE 403
           KQK+DSNIL DASKD+ PLH KDKSKKWQTFVPK ++  T+  E
Sbjct: 361 KQKVDSNILDDASKDVAPLHLKDKSKKWQTFVPKAKACGTTSEE 401

[Close]

BLAST of Lag0032306 vs. NCBI nr
Match: XP_022158793.1 (ATP-dependent DNA helicase SRS2-like protein At4g25120 isoform X3 [Momordica charantia])

HSP 1 Score: 624.8 bits (1610), Expect = 7.7e-175
Identity = 336/402 (83.58%), Postives = 357/402 (88.81%), Query Frame = 0


Query: 1   MFDDENSAITDEERARISRNFRAAKALLARKRPRLFHSHQPISQCSIHNHSANSLPYRVA 60
           M  +ENSAITDEERARISRNFRAAKALLARKRPRL HSH  ISQCS    SAN LP RVA
Sbjct: 1   MSGNENSAITDEERARISRNFRAAKALLARKRPRLLHSHHLISQCSSDKPSANLLPCRVA 60

Query: 61  DFNANEAPVCDNGAKRVPLAEISGNTPLSKSLAIATDASSNGLGLDCLKTPVKSSECSST 120
           DF+ N A  CDNGAKR  LAEISGN P SKS+AIATDASSNGLGLDCLKTPVKS E  ST
Sbjct: 61  DFSVNVAQGCDNGAKRAALAEISGNIPSSKSIAIATDASSNGLGLDCLKTPVKSPEYYST 120

Query: 121 ENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIFHAANQDNDSYNGDLSID 180
            + F APSILDDDFDE+TLEEIDALCEQ SSARAERQGS++IFHAAN D+ SYNGDL+ID
Sbjct: 121 SSYFSAPSILDDDFDETTLEEIDALCEQESSARAERQGSYSIFHAANHDDVSYNGDLNID 180

Query: 181 LESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPEEYSKYLLSLNDRQREAA 240
           LESVIGSESIETKD + SSD  E   EWINSDS T+KIGTMPEEYSKYLLSLNDRQREAA
Sbjct: 181 LESVIGSESIETKDLTCSSDTLESGVEWINSDSVTRKIGTMPEEYSKYLLSLNDRQREAA 240

Query: 241 CSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMTFTTAAASEMRDRVGAVA 300
           C DISIPLMILAGPGSGKTSTMVGRVLMLLNEGI PSNILAMTFTTAAASEMRDRVGAVA
Sbjct: 241 CGDISIPLMILAGPGSGKTSTMVGRVLMLLNEGIKPSNILAMTFTTAAASEMRDRVGAVA 300

Query: 301 GKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRRAIIEAVRLLENEKSKQ 360
           GKKMAKELVISTFHSFSLQLCRLHAEKLERT DFSIYGHGQQRRAIIEAVRLLEN+KS+Q
Sbjct: 301 GKKMAKELVISTFHSFSLQLCRLHAEKLERTTDFSIYGHGQQRRAIIEAVRLLENDKSRQ 360

Query: 361 KLDSNILGDASKDITPLHFKDKSKKWQTFVPKDRSNLTSQSE 403
           K+DSNILG+ASKD+TP +FKDKSKKWQ FVPK ++  T+ +E
Sbjct: 361 KVDSNILGNASKDVTPSNFKDKSKKWQKFVPKAKACGTTSAE 402

[Close]

BLAST of Lag0032306 vs. ExPASy Swiss-Prot
Match: D1KF50 (ATP-dependent DNA helicase SRS2-like protein At4g25120 OS=Arabidopsis thaliana OX=3702 GN=SRS2 PE=1 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.2e-75
Identity = 190/420 (45.24%), Postives = 247/420 (58.81%), Query Frame = 0


Query: 16  RISRNFRAAKALLARKRPRLFHSHQPISQCSIHN--HSANSLPYRVADFNANEAPVCDNG 75
           RIS++FR+AK LL RKRP             I N  +S+N LP R+ +    E+   +  
Sbjct: 19  RISQSFRSAKPLLDRKRP-------------IENAPNSSNPLPQRMKESMDTESVSHNIN 78

Query: 76  AKRVPLAEISGNTPLSK-SLAIATDASSNGLGL--------------------------- 135
               PL E+S NTP  +    I + A  +  GL                           
Sbjct: 79  FNSTPLMELSANTPYKRLKPEIESYADGHPCGLRTPPPRFDLDKEIINGFQTDIYADVGS 138

Query: 136 --DCLKTPVKSSECSSTENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIF 195
             +   TP+K  E  +  N     SILDDDFD+S LEEID +CEQ++   A +  + +I+
Sbjct: 139 LSEAFVTPLKEPERVTLSNGCSTSSILDDDFDDSILEEIDLICEQSARKAACQTPTTSIY 198

Query: 196 HAANQDNDSYNGDLSIDLESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPE 255
              ++DN S +   S+D   V   E  E  DS+      E     I +D A   + +MP+
Sbjct: 199 QTPSKDNKSSDPKASLDFRDV---EKFE-PDSNVKLKLDE-ETPTIAADPAL--LNSMPD 258

Query: 256 EYSKYLLSLNDRQREAACSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMT 315
           E SKY+LSLNDRQR+AACS+IS PLM++AGPGSGKTSTMVGRVL+LLNEG+ PSNILAMT
Sbjct: 259 ECSKYMLSLNDRQRDAACSNISTPLMVIAGPGSGKTSTMVGRVLVLLNEGLLPSNILAMT 318

Query: 316 FTTAAASEMRDRVGAVAGKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQR 375
           FT AA SEMR+R+G  AGKK AK++ ISTFHSFSLQLCR+HA+KL+RT +FS+YGHGQQR
Sbjct: 319 FTKAATSEMRERIGKSAGKKAAKDITISTFHSFSLQLCRMHADKLQRTSEFSVYGHGQQR 378

Query: 376 RAIIEAVRLLENEKSKQKLDS-------NILGDASKDITPLHFKDKSKKWQTFVPKDRSN 397
           RAIIEAVRL E EK      S        + G  +  + P + KD SKKWQ FV + +++
Sbjct: 379 RAIIEAVRLYEEEKKNGSKTSVPCESGEGLNGAGAGAVCPEYAKDLSKKWQKFVTQGKAS 418

[Close]

BLAST of Lag0032306 vs. ExPASy Swiss-Prot
Match: P03018 (DNA helicase II OS=Escherichia coli (strain K12) OX=83333 GN=uvrD PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 9.2e-14
Identity = 58/158 (36.71%), Postives = 85/158 (53.80%), Query Frame = 0


Query: 228 YLL-SLNDRQREAACSDISIPLMILAGPGSGKTSTMVGRVLMLLN-EGISPSNILAMTFT 287
           YLL SLND+QREA  +  S  L++LAG GSGKT  +V R+  L++ E  SP +I+A+TFT
Sbjct: 5   YLLDSLNDKQREAVAAPRS-NLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFT 64

Query: 288 TAAASEMRDRVGAVAGKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRR- 347
             AA+EMR R+G + G      + + TFH  + +L R H        DF I     Q R 
Sbjct: 65  NKAAAEMRHRIGQLMGTSQG-GMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRL 124

Query: 348 --AIIEAVRLLENEKSKQKLDSNILGDASKDITPLHFK 381
              +I+A+ L E +   ++    I     + + P H +
Sbjct: 125 LKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ 160

[Close]

BLAST of Lag0032306 vs. ExPASy Swiss-Prot
Match: Q05311 (DNA helicase II OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=uvrD PE=3 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 1.2e-13
Identity = 58/158 (36.71%), Postives = 85/158 (53.80%), Query Frame = 0


Query: 228 YLL-SLNDRQREAACSDISIPLMILAGPGSGKTSTMVGRVLMLLN-EGISPSNILAMTFT 287
           YLL SLND+QREA  +  S  +++LAG GSGKT  +V R+  LL+ E  SP +I+A+TFT
Sbjct: 5   YLLDSLNDKQREAVAAPRS-NMLVLAGAGSGKTRVLVHRIAWLLSVENNSPYSIMAVTFT 64

Query: 288 TAAASEMRDRVGAVAGKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRR- 347
             AA+EMR R+G + G      + + TFH  + +L R H        DF I     Q R 
Sbjct: 65  NKAAAEMRHRIGQLMGTSQG-GMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQMRL 124

Query: 348 --AIIEAVRLLENEKSKQKLDSNILGDASKDITPLHFK 381
              +I+A+ L E +   ++    I     + + P H +
Sbjct: 125 LKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ 160

[Close]

BLAST of Lag0032306 vs. ExPASy Swiss-Prot
Match: P0A5A4 (ATP-dependent DNA helicase UvrD1 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=uvrD1 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 3.5e-13
Identity = 51/127 (40.16%), Postives = 76/127 (59.84%), Query Frame = 0


Query: 222 PEEYSKYLLSLNDRQREAACSDISIPLMILAGPGSGKTSTMVGRVLMLL-NEGISPSNIL 281
           P    + L  LN +QR+A   + S PL+I+AG GSGKT+ +  R+  L+   G+    IL
Sbjct: 13  PSPADQLLDGLNPQQRQAVVHEGS-PLLIVAGAGSGKTAVLTRRIAYLMAARGVGVGQIL 72

Query: 282 AMTFTTAAASEMRDRVGAVAGKKMAKELVISTFHSFSLQLCRLHAEKLE-RTPDFSIYGH 341
           A+TFT  AA+EMR+RV  + G+K A+ + +STFHS  +++ R  A  +E    +FSIY  
Sbjct: 73  AITFTNKAAAEMRERVVGLVGEK-ARYMWVSTFHSTCVRILRNQAALIEGLNSNFSIYDA 132

Query: 342 GQQRRAI 347
              RR +
Sbjct: 133 DDSRRLL 137

[Close]

BLAST of Lag0032306 vs. ExPASy Swiss-Prot
Match: P9WMQ0 (ATP-dependent DNA helicase UvrD1 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=uvrD1 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 3.5e-13
Identity = 51/127 (40.16%), Postives = 76/127 (59.84%), Query Frame = 0


Query: 222 PEEYSKYLLSLNDRQREAACSDISIPLMILAGPGSGKTSTMVGRVLMLL-NEGISPSNIL 281
           P    + L  LN +QR+A   + S PL+I+AG GSGKT+ +  R+  L+   G+    IL
Sbjct: 13  PSPADQLLDGLNPQQRQAVVHEGS-PLLIVAGAGSGKTAVLTRRIAYLMAARGVGVGQIL 72

Query: 282 AMTFTTAAASEMRDRVGAVAGKKMAKELVISTFHSFSLQLCRLHAEKLE-RTPDFSIYGH 341
           A+TFT  AA+EMR+RV  + G+K A+ + +STFHS  +++ R  A  +E    +FSIY  
Sbjct: 73  AITFTNKAAAEMRERVVGLVGEK-ARYMWVSTFHSTCVRILRNQAALIEGLNSNFSIYDA 132

Query: 342 GQQRRAI 347
              RR +
Sbjct: 133 DDSRRLL 137

[Close]

BLAST of Lag0032306 vs. ExPASy TrEMBL
Match: A0A6J1K3K3 (DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111490846 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 8.0e-178
Identity = 339/404 (83.91%), Postives = 356/404 (88.12%), Query Frame = 0


Query: 1   MFDDENSAITDEERARISRNFRAAKALLARKRPRLFHSHQPISQCSIHNHSANSLP--YR 60
           M +DENS IT+EER RISRNFRAAKALLARKRPRL HSHQPISQCS   HSA  LP   R
Sbjct: 1   MIEDENSGITEEERDRISRNFRAAKALLARKRPRLLHSHQPISQCSNRIHSATPLPNSSR 60

Query: 61  VADFNANEAPVCDNGAKRVPLAEISGNTPLSKSLAIATDASSNGLGLDCLKTPVKSSECS 120
           V+D NAN+APVCDNGAKRVPL+EISGNTPLSKS AIA DA+S GLGLDC KTP+KS ECS
Sbjct: 61  VSDLNANDAPVCDNGAKRVPLSEISGNTPLSKSFAIAVDATSKGLGLDCFKTPLKSYECS 120

Query: 121 STENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIFHAANQDNDSYNGDLS 180
           ST NSF APSILDDDFDESTLEEIDALCEQNSSARAER  SH+IFHA N DN SYN D S
Sbjct: 121 STRNSFSAPSILDDDFDESTLEEIDALCEQNSSARAERDDSHSIFHAENHDNGSYNDDPS 180

Query: 181 IDLESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPEEYSKYLLSLNDRQRE 240
           IDLE V GSES E K  S SSDA ELR+EWINSDSATKKIG MP EYSKYLLSLNDRQ+E
Sbjct: 181 IDLEFVNGSESSENKGLSCSSDALELRSEWINSDSATKKIGAMPAEYSKYLLSLNDRQQE 240

Query: 241 AACSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMTFTTAAASEMRDRVGA 300
           AACSDISIPLMILAGPGSGKTSTMVGRVLMLL+EGI P+NILAMTFTTAAASEMRDRVGA
Sbjct: 241 AACSDISIPLMILAGPGSGKTSTMVGRVLMLLSEGIGPANILAMTFTTAAASEMRDRVGA 300

Query: 301 VAGKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRRAIIEAVRLLENEKS 360
           V GKKMAKELVISTFHSFSLQLCRLHAEKLERT DFSIYGHGQQRRAIIEAVRLLENEKS
Sbjct: 301 VTGKKMAKELVISTFHSFSLQLCRLHAEKLERTSDFSIYGHGQQRRAIIEAVRLLENEKS 360

Query: 361 KQKLDSNILGDASKDITPLHFKDKSKKWQTFVPKDRSNLTSQSE 403
           KQK+DSNIL DASKD+ PLH KDKSKKWQTFVPK ++  T+  E
Sbjct: 361 KQKVDSNILDDASKDVAPLHLKDKSKKWQTFVPKAKACGTTSEE 404

[Close]

BLAST of Lag0032306 vs. ExPASy TrEMBL
Match: A0A6J1E0F7 (DNA helicase OS=Momordica charantia OX=3673 GN=LOC111025247 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 3.7e-175
Identity = 336/402 (83.58%), Postives = 357/402 (88.81%), Query Frame = 0


Query: 1   MFDDENSAITDEERARISRNFRAAKALLARKRPRLFHSHQPISQCSIHNHSANSLPYRVA 60
           M  +ENSAITDEERARISRNFRAAKALLARKRPRL HSH  ISQCS    SAN LP RVA
Sbjct: 1   MSGNENSAITDEERARISRNFRAAKALLARKRPRLLHSHHLISQCSSDKPSANLLPCRVA 60

Query: 61  DFNANEAPVCDNGAKRVPLAEISGNTPLSKSLAIATDASSNGLGLDCLKTPVKSSECSST 120
           DF+ N A  CDNGAKR  LAEISGN P SKS+AIATDASSNGLGLDCLKTPVKS E  ST
Sbjct: 61  DFSVNVAQGCDNGAKRAALAEISGNIPSSKSIAIATDASSNGLGLDCLKTPVKSPEYYST 120

Query: 121 ENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIFHAANQDNDSYNGDLSID 180
            + F APSILDDDFDE+TLEEIDALCEQ SSARAERQGS++IFHAAN D+ SYNGDL+ID
Sbjct: 121 SSYFSAPSILDDDFDETTLEEIDALCEQESSARAERQGSYSIFHAANHDDVSYNGDLNID 180

Query: 181 LESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPEEYSKYLLSLNDRQREAA 240
           LESVIGSESIETKD + SSD  E   EWINSDS T+KIGTMPEEYSKYLLSLNDRQREAA
Sbjct: 181 LESVIGSESIETKDLTCSSDTLESGVEWINSDSVTRKIGTMPEEYSKYLLSLNDRQREAA 240

Query: 241 CSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMTFTTAAASEMRDRVGAVA 300
           C DISIPLMILAGPGSGKTSTMVGRVLMLLNEGI PSNILAMTFTTAAASEMRDRVGAVA
Sbjct: 241 CGDISIPLMILAGPGSGKTSTMVGRVLMLLNEGIKPSNILAMTFTTAAASEMRDRVGAVA 300

Query: 301 GKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRRAIIEAVRLLENEKSKQ 360
           GKKMAKELVISTFHSFSLQLCRLHAEKLERT DFSIYGHGQQRRAIIEAVRLLEN+KS+Q
Sbjct: 301 GKKMAKELVISTFHSFSLQLCRLHAEKLERTTDFSIYGHGQQRRAIIEAVRLLENDKSRQ 360

Query: 361 KLDSNILGDASKDITPLHFKDKSKKWQTFVPKDRSNLTSQSE 403
           K+DSNILG+ASKD+TP +FKDKSKKWQ FVPK ++  T+ +E
Sbjct: 361 KVDSNILGNASKDVTPSNFKDKSKKWQKFVPKAKACGTTSAE 402

[Close]

BLAST of Lag0032306 vs. ExPASy TrEMBL
Match: A0A6J1DWU7 (DNA helicase OS=Momordica charantia OX=3673 GN=LOC111025247 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 3.7e-175
Identity = 336/402 (83.58%), Postives = 357/402 (88.81%), Query Frame = 0


Query: 1   MFDDENSAITDEERARISRNFRAAKALLARKRPRLFHSHQPISQCSIHNHSANSLPYRVA 60
           M  +ENSAITDEERARISRNFRAAKALLARKRPRL HSH  ISQCS    SAN LP RVA
Sbjct: 1   MSGNENSAITDEERARISRNFRAAKALLARKRPRLLHSHHLISQCSSDKPSANLLPCRVA 60

Query: 61  DFNANEAPVCDNGAKRVPLAEISGNTPLSKSLAIATDASSNGLGLDCLKTPVKSSECSST 120
           DF+ N A  CDNGAKR  LAEISGN P SKS+AIATDASSNGLGLDCLKTPVKS E  ST
Sbjct: 61  DFSVNVAQGCDNGAKRAALAEISGNIPSSKSIAIATDASSNGLGLDCLKTPVKSPEYYST 120

Query: 121 ENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIFHAANQDNDSYNGDLSID 180
            + F APSILDDDFDE+TLEEIDALCEQ SSARAERQGS++IFHAAN D+ SYNGDL+ID
Sbjct: 121 SSYFSAPSILDDDFDETTLEEIDALCEQESSARAERQGSYSIFHAANHDDVSYNGDLNID 180

Query: 181 LESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPEEYSKYLLSLNDRQREAA 240
           LESVIGSESIETKD + SSD  E   EWINSDS T+KIGTMPEEYSKYLLSLNDRQREAA
Sbjct: 181 LESVIGSESIETKDLTCSSDTLESGVEWINSDSVTRKIGTMPEEYSKYLLSLNDRQREAA 240

Query: 241 CSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMTFTTAAASEMRDRVGAVA 300
           C DISIPLMILAGPGSGKTSTMVGRVLMLLNEGI PSNILAMTFTTAAASEMRDRVGAVA
Sbjct: 241 CGDISIPLMILAGPGSGKTSTMVGRVLMLLNEGIKPSNILAMTFTTAAASEMRDRVGAVA 300

Query: 301 GKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRRAIIEAVRLLENEKSKQ 360
           GKKMAKELVISTFHSFSLQLCRLHAEKLERT DFSIYGHGQQRRAIIEAVRLLEN+KS+Q
Sbjct: 301 GKKMAKELVISTFHSFSLQLCRLHAEKLERTTDFSIYGHGQQRRAIIEAVRLLENDKSRQ 360

Query: 361 KLDSNILGDASKDITPLHFKDKSKKWQTFVPKDRSNLTSQSE 403
           K+DSNILG+ASKD+TP +FKDKSKKWQ FVPK ++  T+ +E
Sbjct: 361 KVDSNILGNASKDVTPSNFKDKSKKWQKFVPKAKACGTTSAE 402

[Close]

BLAST of Lag0032306 vs. ExPASy TrEMBL
Match: A0A6J1E0F2 (DNA helicase OS=Momordica charantia OX=3673 GN=LOC111025247 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 3.7e-175
Identity = 336/402 (83.58%), Postives = 357/402 (88.81%), Query Frame = 0


Query: 1   MFDDENSAITDEERARISRNFRAAKALLARKRPRLFHSHQPISQCSIHNHSANSLPYRVA 60
           M  +ENSAITDEERARISRNFRAAKALLARKRPRL HSH  ISQCS    SAN LP RVA
Sbjct: 1   MSGNENSAITDEERARISRNFRAAKALLARKRPRLLHSHHLISQCSSDKPSANLLPCRVA 60

Query: 61  DFNANEAPVCDNGAKRVPLAEISGNTPLSKSLAIATDASSNGLGLDCLKTPVKSSECSST 120
           DF+ N A  CDNGAKR  LAEISGN P SKS+AIATDASSNGLGLDCLKTPVKS E  ST
Sbjct: 61  DFSVNVAQGCDNGAKRAALAEISGNIPSSKSIAIATDASSNGLGLDCLKTPVKSPEYYST 120

Query: 121 ENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIFHAANQDNDSYNGDLSID 180
            + F APSILDDDFDE+TLEEIDALCEQ SSARAERQGS++IFHAAN D+ SYNGDL+ID
Sbjct: 121 SSYFSAPSILDDDFDETTLEEIDALCEQESSARAERQGSYSIFHAANHDDVSYNGDLNID 180

Query: 181 LESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPEEYSKYLLSLNDRQREAA 240
           LESVIGSESIETKD + SSD  E   EWINSDS T+KIGTMPEEYSKYLLSLNDRQREAA
Sbjct: 181 LESVIGSESIETKDLTCSSDTLESGVEWINSDSVTRKIGTMPEEYSKYLLSLNDRQREAA 240

Query: 241 CSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMTFTTAAASEMRDRVGAVA 300
           C DISIPLMILAGPGSGKTSTMVGRVLMLLNEGI PSNILAMTFTTAAASEMRDRVGAVA
Sbjct: 241 CGDISIPLMILAGPGSGKTSTMVGRVLMLLNEGIKPSNILAMTFTTAAASEMRDRVGAVA 300

Query: 301 GKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRRAIIEAVRLLENEKSKQ 360
           GKKMAKELVISTFHSFSLQLCRLHAEKLERT DFSIYGHGQQRRAIIEAVRLLEN+KS+Q
Sbjct: 301 GKKMAKELVISTFHSFSLQLCRLHAEKLERTTDFSIYGHGQQRRAIIEAVRLLENDKSRQ 360

Query: 361 KLDSNILGDASKDITPLHFKDKSKKWQTFVPKDRSNLTSQSE 403
           K+DSNILG+ASKD+TP +FKDKSKKWQ FVPK ++  T+ +E
Sbjct: 361 KVDSNILGNASKDVTPSNFKDKSKKWQKFVPKAKACGTTSAE 402

[Close]

BLAST of Lag0032306 vs. ExPASy TrEMBL
Match: A0A6J1DX37 (DNA helicase OS=Momordica charantia OX=3673 GN=LOC111025247 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 3.7e-175
Identity = 336/402 (83.58%), Postives = 357/402 (88.81%), Query Frame = 0


Query: 1   MFDDENSAITDEERARISRNFRAAKALLARKRPRLFHSHQPISQCSIHNHSANSLPYRVA 60
           M  +ENSAITDEERARISRNFRAAKALLARKRPRL HSH  ISQCS    SAN LP RVA
Sbjct: 1   MSGNENSAITDEERARISRNFRAAKALLARKRPRLLHSHHLISQCSSDKPSANLLPCRVA 60

Query: 61  DFNANEAPVCDNGAKRVPLAEISGNTPLSKSLAIATDASSNGLGLDCLKTPVKSSECSST 120
           DF+ N A  CDNGAKR  LAEISGN P SKS+AIATDASSNGLGLDCLKTPVKS E  ST
Sbjct: 61  DFSVNVAQGCDNGAKRAALAEISGNIPSSKSIAIATDASSNGLGLDCLKTPVKSPEYYST 120

Query: 121 ENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIFHAANQDNDSYNGDLSID 180
            + F APSILDDDFDE+TLEEIDALCEQ SSARAERQGS++IFHAAN D+ SYNGDL+ID
Sbjct: 121 SSYFSAPSILDDDFDETTLEEIDALCEQESSARAERQGSYSIFHAANHDDVSYNGDLNID 180

Query: 181 LESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPEEYSKYLLSLNDRQREAA 240
           LESVIGSESIETKD + SSD  E   EWINSDS T+KIGTMPEEYSKYLLSLNDRQREAA
Sbjct: 181 LESVIGSESIETKDLTCSSDTLESGVEWINSDSVTRKIGTMPEEYSKYLLSLNDRQREAA 240

Query: 241 CSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMTFTTAAASEMRDRVGAVA 300
           C DISIPLMILAGPGSGKTSTMVGRVLMLLNEGI PSNILAMTFTTAAASEMRDRVGAVA
Sbjct: 241 CGDISIPLMILAGPGSGKTSTMVGRVLMLLNEGIKPSNILAMTFTTAAASEMRDRVGAVA 300

Query: 301 GKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQRRAIIEAVRLLENEKSKQ 360
           GKKMAKELVISTFHSFSLQLCRLHAEKLERT DFSIYGHGQQRRAIIEAVRLLEN+KS+Q
Sbjct: 301 GKKMAKELVISTFHSFSLQLCRLHAEKLERTTDFSIYGHGQQRRAIIEAVRLLENDKSRQ 360

Query: 361 KLDSNILGDASKDITPLHFKDKSKKWQTFVPKDRSNLTSQSE 403
           K+DSNILG+ASKD+TP +FKDKSKKWQ FVPK ++  T+ +E
Sbjct: 361 KVDSNILGNASKDVTPSNFKDKSKKWQKFVPKAKACGTTSAE 402

[Close]

BLAST of Lag0032306 vs. TAIR 10
Match: AT4G25120.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 284.3 bits (726), Expect = 2.3e-76
Identity = 190/420 (45.24%), Postives = 247/420 (58.81%), Query Frame = 0


Query: 16  RISRNFRAAKALLARKRPRLFHSHQPISQCSIHN--HSANSLPYRVADFNANEAPVCDNG 75
           RIS++FR+AK LL RKRP             I N  +S+N LP R+ +    E+   +  
Sbjct: 19  RISQSFRSAKPLLDRKRP-------------IENAPNSSNPLPQRMKESMDTESVSHNIN 78

Query: 76  AKRVPLAEISGNTPLSK-SLAIATDASSNGLGL--------------------------- 135
               PL E+S NTP  +    I + A  +  GL                           
Sbjct: 79  FNSTPLMELSANTPYKRLKPEIESYADGHPCGLRTPPPRFDLDKEIINGFQTDIYADVGS 138

Query: 136 --DCLKTPVKSSECSSTENSFLAPSILDDDFDESTLEEIDALCEQNSSARAERQGSHAIF 195
             +   TP+K  E  +  N     SILDDDFD+S LEEID +CEQ++   A +  + +I+
Sbjct: 139 LSEAFVTPLKEPERVTLSNGCSTSSILDDDFDDSILEEIDLICEQSARKAACQTPTTSIY 198

Query: 196 HAANQDNDSYNGDLSIDLESVIGSESIETKDSSRSSDASELRAEWINSDSATKKIGTMPE 255
              ++DN S +   S+D   V   E  E  DS+      E     I +D A   + +MP+
Sbjct: 199 QTPSKDNKSSDPKASLDFRDV---EKFE-PDSNVKLKLDE-ETPTIAADPAL--LNSMPD 258

Query: 256 EYSKYLLSLNDRQREAACSDISIPLMILAGPGSGKTSTMVGRVLMLLNEGISPSNILAMT 315
           E SKY+LSLNDRQR+AACS+IS PLM++AGPGSGKTSTMVGRVL+LLNEG+ PSNILAMT
Sbjct: 259 ECSKYMLSLNDRQRDAACSNISTPLMVIAGPGSGKTSTMVGRVLVLLNEGLLPSNILAMT 318

Query: 316 FTTAAASEMRDRVGAVAGKKMAKELVISTFHSFSLQLCRLHAEKLERTPDFSIYGHGQQR 375
           FT AA SEMR+R+G  AGKK AK++ ISTFHSFSLQLCR+HA+KL+RT +FS+YGHGQQR
Sbjct: 319 FTKAATSEMRERIGKSAGKKAAKDITISTFHSFSLQLCRMHADKLQRTSEFSVYGHGQQR 378

Query: 376 RAIIEAVRLLENEKSKQKLDS-------NILGDASKDITPLHFKDKSKKWQTFVPKDRSN 397
           RAIIEAVRL E EK      S        + G  +  + P + KD SKKWQ FV + +++
Sbjct: 379 RAIIEAVRLYEEEKKNGSKTSVPCESGEGLNGAGAGAVCPEYAKDLSKKWQKFVTQGKAS 418

The following BLAST results are available for this feature:

BLAST of Lag0032306 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022995240.1	1.7e-177	83.91	ATP-dependent DNA helicase SRS2-like protein At4g25120 [Cucurbita maxima]	[more]
KAG6605657.1	1.4e-176	83.66	ATP-dependent DNA helicase SRS2-like protein, partial [Cucurbita argyrosperma su...	[more]
XP_023532520.1	4.1e-176	83.66	ATP-dependent DNA helicase SRS2-like protein At4g25120 [Cucurbita pepo subsp. pe...	[more]
KAG7035567.1	7.7e-175	83.42	ATP-dependent DNA helicase SRS2-like protein [Cucurbita argyrosperma subsp. argy...	[more]
XP_022158793.1	7.7e-175	83.58	ATP-dependent DNA helicase SRS2-like protein At4g25120 isoform X3 [Momordica cha...	[more]

BLAST of Lag0032306 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D1KF50	3.2e-75	45.24	ATP-dependent DNA helicase SRS2-like protein At4g25120 OS=Arabidopsis thaliana O...	[more]
P03018	9.2e-14	36.71	DNA helicase II OS=Escherichia coli (strain K12) OX=83333 GN=uvrD PE=1 SV=1	[more]
Q05311	1.2e-13	36.71	DNA helicase II OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) ...	[more]
P0A5A4	3.5e-13	40.16	ATP-dependent DNA helicase UvrD1 OS=Mycobacterium bovis (strain ATCC BAA-935 / A...	[more]
P9WMQ0	3.5e-13	40.16	ATP-dependent DNA helicase UvrD1 OS=Mycobacterium tuberculosis (strain CDC 1551 ...	[more]

BLAST of Lag0032306 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K3K3	8.0e-178	83.91	DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111490846 PE=3 SV=1	[more]
A0A6J1E0F7	3.7e-175	83.58	DNA helicase OS=Momordica charantia OX=3673 GN=LOC111025247 PE=3 SV=1	[more]
A0A6J1DWU7	3.7e-175	83.58	DNA helicase OS=Momordica charantia OX=3673 GN=LOC111025247 PE=3 SV=1	[more]
A0A6J1E0F2	3.7e-175	83.58	DNA helicase OS=Momordica charantia OX=3673 GN=LOC111025247 PE=3 SV=1	[more]
A0A6J1DX37	3.7e-175	83.58	DNA helicase OS=Momordica charantia OX=3673 GN=LOC111025247 PE=3 SV=1	[more]

BLAST of Lag0032306 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25120.1	2.3e-76	45.24	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 224..385 e-value: 9.8E-37 score: 128.3
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 224..360
IPR014016	UvrD-like helicase, ATP-binding domain	PFAM	PF00580	UvrD-helicase	coord: 232..406 e-value: 2.3E-28 score: 99.7
IPR014016	UvrD-like helicase, ATP-binding domain	PROSITE	PS51198	UVRD_HELICASE_ATP_BIND	coord: 231..574 score: 21.181213
None	No IPR available	PANTHER	PTHR11070:SF2	ATP-DEPENDENT DNA HELICASE SRS2	coord: 231..411
None	No IPR available	CDD	cd17932	DEXQc_UvrD	coord: 233..380 e-value: 1.80317E-32 score: 121.469
IPR000212	DNA helicase, UvrD/REP type	PANTHER	PTHR11070	UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER	coord: 231..411

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0032306.1	Lag0032306.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032508	DNA duplex unwinding
biological_process	GO:0036292	DNA rewinding
biological_process	GO:0000725	recombinational repair
cellular_component	GO:0005634	nucleus
molecular_function	GO:0043138	3'-5' DNA helicase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003678	DNA helicase activity