Lag0032274 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032274
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionNO-associated protein 1, chloroplastic/mitochondrial
Locationchr11: 29333954 .. 29337666 (-)
RNA-Seq ExpressionLag0032274
SyntenyLag0032274
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGATTGGAGAGGACTTGAAACAGTGCGTCAATTGAAGATAAATTTTGAGGAAGAGCAAAGGTGCGTTAAAACTCTTGTTGATTGACAAGTTATGTTGTTTTGTTAAATAACCTTTGTCATCTGCTCTGGAATTTATTGTATTGCTTTTTTCAGTTTTTGTCCTCTGGGATGGAATAGAATGTTATTCTAGATGTGTTTTGGTTCACGTAAATCAGGCTGGCTAATAGGTGCATCACTGCATCACAGGAAATAAGAACTTATTTCTCCTTTCATTTTGTACAGGCCTGCTTGTGATGTGGCTATATCAGGCTTGGGATGGATTGCTGTCGAACAAAAGGAGTCGCTCCAAAATACTCACTCAGATTTAGAATCAACAGTTGATGAACTTGAGTTGGCTGTTCATGTCCCCAAGCCAGTGGAGATTTTCGTGAGGCCTTCCATGCCTGTTGGCAAAGCTGGAGCAGAATGGTATCAGTACCGCGATTTAACAGAGGAGGAAGAGGAAGCAAGACCAAGATGGTATTTTTAGAGTTTTTTAGGTACTCTCTACCTCTTCGTCTTTATATATTTTGCAATTAATCGTATAACTGGCCAGCTGCTTCTGCTGCCTAGGCAAATGAAATGTAGATACAGAGAGATGGCTGATATATCGGTTAATTTATAAGCTCATGAATGTAATAGAATAGAACTTTGAAGATTCTGAATCAGAAAATTTTTAATGGGATCGACTTAAGCATCCAAAGTCTTTGTGAAGCTGCTTTTCCACATTTTCATCAATTGGGAAGTGTTTTTCTTTTAAAAACCTTTTGGCTCTCTGGGAATATCTGACCTTCTCTCTTTGTATATGATCTTTTGTTCTAATACATTTTAAGTCTCTTATATATATGTATATATATAAATATAAGATTACTGGTAAAATTAGAATAAACATATATAATGCTCTATGCATCTATTGAACTAGATATTTCTTTTAATATGCTTTACTCGAGTTGGATTCATATCTATTAAGGGTTGATTTATATCTATTAACGGCACTGAACTTTATTTGAATAAGGAACTCGTGATAAACTGTTGTTTGAACCATCATTTGGTTGGAAAGAGAAAGTAGATAGAAGTTTTAATTGAGCTTACAGTTATAAACTTGCTTCTTTTCATTTATTATTATTATTATTATTATTTTTACATTCATGTCTCTCCAAATCTTCCTAACAAGTGAGAAGAAGGTCAAAGTTAAGGTATATACCTTGTCAAGCTTTTCTCCATGTGCATTTATTTATAATAGAAGACATTTGTAGATTTTTCGTCCCTTCGCCTCCCCGGAAATGAGTTGTTCGAGTTCTAGTGGTCTTTTCAGTTCCATTTTTTGTCCAATGAACAACGATAAACGAAGGTTTGATTTCGACATTCGACGATTTTCGTCCATGACATTTGTAGAAGGCTAAAAAATGGTCGGGTTATTTGCGATATAACAGAGGTCCAATTTCGATAGATGGTTGACAACAGTTTGAGTTTTTGAGTCTTTTCTCGGTTCGTCTAGTAGACTTCTGATTTGTATAATGACATTCCTTTGATGGTGGTCCAATTCTTTGCCTTTCCGACTATTTATGTTTTTTCAATAAATGTTTCTACAAATCAAACAAAGAAGGTACAATATATACAAAGTTTATTTTTTAGTTTCGGAAATTTATAGGTTTCATTTCCAAATTTTTTAGTTTCATACATTTATAGGTTTCATTTCTAAATTTTATTTAATTTTAGGGTTTATTTCAAATAATCTGAGAACAACAGTAGTTGTATGTACTAATACACTTGAGCCATCATGAGATTGAACTAATATTGTAGCATTCCTATATTGTTATGCATGACTTGTTGGGTTTATTTTAAATAGTGAATGAAGAAAAATATACAAGGTTTATATTCGAGATTCATATATTTATAGGGTTTATAACAAACAATCAGAGATACGTATATATAGCTGGGTGACATCATCAAACTTTCAAGAAGAAGACAAAATCGAGAGACTCGAGGATAGTGCAGACATATCCCATCTATCTTTTCTGCTTTCCAGTTGTATTTTGTCGCTTTTTAGTTTTATGTAAATTATTGTATCTTTACTTTATTTCCTTTGCCGATTACAAAGAGATAAGGATAAGAAGGGTTCCTGTCCCACCATATATTATCGTAATCTTTTCTTTATCAAAAGAAAGATTCCAAAGAGATAACTTGCCAGTCAGGGGTCCTGTCCTGTCGGCCACTCTTGGAATTATTTCTCAATGTACTCAATTTTGTCGATGCCACGTGTAAAAACCTGAATAGTGATTTGATATCTGTGCAATTGCACAGGACAAAATCAGATCTCTATATAAGGGCACGTAGTCTGACCATCAATGGTCAAAATCAGATCTCTATATAAGGGCACGTAGTCCGACCATCAATGGTATCTGAGCCATAAAGCTCAACTTCTCTCTAAAGATTTCTCTCTCTCATGGAGTAAACTCCTTTTCAGGATCTGAGAAGGATGATAGCTTATCTCTAATGATATTCTTCTAAGGTTTGAGTTTATTCCTAATTCATTCTTCTGTGTGTTATTCTAACTATGGTTGTGTCTCTTCGTTAGAAATCTTGCAAAATGGAAATAAAATTTATGACAATTCTTAATCATAAGCATAAGAAAGGCTGGTTAACCTTCATGCTAGATTAAGAAGAAATCATAAAAGTTATGAAAATTTTGTAAGGTTCCTAATCTTAAATCGATTGATATAAATAGTTAGAAAAATCGAAAGAAGAATTGATTAACATAGATTTAAATTTTATCAGAATATTATTAGTTTGCAGCTATCTGAGTTCTCAGGTTCGCCTTCTCCTTCTCCCTCATGCTTTCTAAATTCAAGAAAAAGTTTTCCTTTGGCCCAACGATCATAGATGCCACTACTTCCTCTTCTTCCTCTTCAAAAAATCCAAAACCTAATGCTAAGGAATTGGAAAAAGCTCTATCCAGACATAAAAGGATAGAATCTCAAATATCTCAAGTTGAGGATAAACTTCAGATTTGGAGTATTCCCAAGCTTGAAGTCAATACTATCTACAAGATAAGTCCATTCAATTTCTTAGAAACAGATGTTATCAGAGTTTCTGAAGATAATATTCCAATGGAGGATGATTTTACAGAATATCAGCTCCTAATCAATGAAGACATCGATGTGTATCGAATGCATTTCAAATAGCTACATATCGGATGTATTCAAGTTGCCATCAAACCTCTCTTTCGGACGGGATTAAACGTACCAGTCTATATTAAGAGATAAAAGGCATTTGAACTTCTCTGATTCCTTATTAGGGTTAGTTTAATCAAATCTTGAAAACGGACCAGTCTTCTTTTGATGCAAGCCTGACTTTACAGTCGCTCTTCAAGACAAGAATATCTTTGACGTTCTTTGTCTGGATATTCGAGGAAAGGGATTACGGCTCAAAGACGGATCATATCCTTTTGTCATCCTATTCAGGATGTATTACAAATTGATGCATACCAATATTTCTCCAAACGCCTTGGGTGTATCTCCTAGAAATTATACCATGCTCATGGAAGTCAATCTTGAGAGATCCTCGATGACTATTCCCAGAAAACTGAATTGGTGTGATCTCACCGTAAACCCTGTTTGGACCTTAGAGCAAGCAACTGCTCCAAGGAACAAACAGAGCAACTATTGA

mRNA sequence

ATGGATGATTGGAGAGGACTTGAAACAGTGCGTCAATTGAAGATAAATTTTGAGGAAGAGCAAAGGCCTGCTTGTGATGTGGCTATATCAGGCTTGGGATGGATTGCTGTCGAACAAAAGGAGTCGCTCCAAAATACTCACTCAGATTTAGAATCAACAGTTGATGAACTTGAGTTGGCTGTTCATGTCCCCAAGCCAGTGGAGATTTTCGTGAGGCCTTCCATGCCTGTTGGCAAAGCTGGAGCAGAATGGTATCAGTACCGCGATTTAACAGAGGAGGAAGAGGAAGCAAGACCAAGATGGTTCGCCTTCTCCTTCTCCCTCATGCTTTCTAAATTCAAGAAAAAGTTTTCCTTTGGCCCAACGATCATAGATGCCACTACTTCCTCTTCTTCCTCTTCAAAAAATCCAAAACCTAATGCTAAGGAATTGGAAAAAGCTCTATCCAGACATAAAAGGATAGAATCTCAAATATCTCAAGTTGAGGATAAACTTCAGATTTGGAGTATTCCCAAGCTTGAAGTCAATACTATCTACAAGATAAGTCCATTCAATTTCTTAGAAACAGATGTTATCAGAGTTTCTGAAGATAATATTCCAATGGAGGATGATTTTACAGAATATCAGCTCCTAATCAATGAAGACATCGATCCTGACTTTACAGTCGCTCTTCAAGACAAGAATATCTTTGACGTTCTTTGTCTGGATATTCGAGGAAAGGGATTACGGCTCAAAGACGGATCATATCCTTTTGTCATCCTATTCAGGATGTATTACAAATTGATGCATACCAATATTTCTCCAAACGCCTTGGGTGTATCTCCTAGAAATTATACCATGCTCATGGAAGTCAATCTTGAGAGATCCTCGATGACTATTCCCAGAAAACTGAATTGGTGTGATCTCACCGTAAACCCTGTTTGGACCTTAGAGCAAGCAACTGCTCCAAGGAACAAACAGAGCAACTATTGA

Coding sequence (CDS)

ATGGATGATTGGAGAGGACTTGAAACAGTGCGTCAATTGAAGATAAATTTTGAGGAAGAGCAAAGGCCTGCTTGTGATGTGGCTATATCAGGCTTGGGATGGATTGCTGTCGAACAAAAGGAGTCGCTCCAAAATACTCACTCAGATTTAGAATCAACAGTTGATGAACTTGAGTTGGCTGTTCATGTCCCCAAGCCAGTGGAGATTTTCGTGAGGCCTTCCATGCCTGTTGGCAAAGCTGGAGCAGAATGGTATCAGTACCGCGATTTAACAGAGGAGGAAGAGGAAGCAAGACCAAGATGGTTCGCCTTCTCCTTCTCCCTCATGCTTTCTAAATTCAAGAAAAAGTTTTCCTTTGGCCCAACGATCATAGATGCCACTACTTCCTCTTCTTCCTCTTCAAAAAATCCAAAACCTAATGCTAAGGAATTGGAAAAAGCTCTATCCAGACATAAAAGGATAGAATCTCAAATATCTCAAGTTGAGGATAAACTTCAGATTTGGAGTATTCCCAAGCTTGAAGTCAATACTATCTACAAGATAAGTCCATTCAATTTCTTAGAAACAGATGTTATCAGAGTTTCTGAAGATAATATTCCAATGGAGGATGATTTTACAGAATATCAGCTCCTAATCAATGAAGACATCGATCCTGACTTTACAGTCGCTCTTCAAGACAAGAATATCTTTGACGTTCTTTGTCTGGATATTCGAGGAAAGGGATTACGGCTCAAAGACGGATCATATCCTTTTGTCATCCTATTCAGGATGTATTACAAATTGATGCATACCAATATTTCTCCAAACGCCTTGGGTGTATCTCCTAGAAATTATACCATGCTCATGGAAGTCAATCTTGAGAGATCCTCGATGACTATTCCCAGAAAACTGAATTGGTGTGATCTCACCGTAAACCCTGTTTGGACCTTAGAGCAAGCAACTGCTCCAAGGAACAAACAGAGCAACTATTGA

Protein sequence

MDDWRGLETVRQLKINFEEEQRPACDVAISGLGWIAVEQKESLQNTHSDLESTVDELELAVHVPKPVEIFVRPSMPVGKAGAEWYQYRDLTEEEEEARPRWFAFSFSLMLSKFKKKFSFGPTIIDATTSSSSSSKNPKPNAKELEKALSRHKRIESQISQVEDKLQIWSIPKLEVNTIYKISPFNFLETDVIRVSEDNIPMEDDFTEYQLLINEDIDPDFTVALQDKNIFDVLCLDIRGKGLRLKDGSYPFVILFRMYYKLMHTNISPNALGVSPRNYTMLMEVNLERSSMTIPRKLNWCDLTVNPVWTLEQATAPRNKQSNY
Homology
BLAST of Lag0032274 vs. NCBI nr
Match: KAA0033914.1 (polyprotein [Cucumis melo var. makuwa] >TYK01296.1 polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 229.9 bits (585), Expect = 3.0e-56
Identity = 127/279 (45.52%), Postives = 170/279 (60.93%), Query Frame = 0

Query: 109 MLSKFKKKFSFGPTIIDATTSSSSSSKNPKPNAKELEKALSRHKRIESQISQVEDKLQIW 168
           MLSK KKK S    +I+   +SSSSS N   N++ LE+ LS++ R+E+Q S +E++LQ W
Sbjct: 1   MLSKLKKKLSLNSLVIE---TSSSSSNNFGINSENLEETLSKYSRVENQSSSIENRLQNW 60

Query: 169 SIPKLEVNTIYKISPFNFLETDVIRVSEDNIPMEDDFTEYQLLINEDID----------- 228
           SIP+LE   IY    FNF E DV+R+SEDNIP+ +DF++Y+LL NE+I+           
Sbjct: 61  SIPRLEPKEIYAHPFFNFREKDVVRISEDNIPVSEDFSKYRLLANENIELCRKHNYRHLH 120

Query: 229 ------------------------------------------------------PDFTVA 288
                                                                 P FTVA
Sbjct: 121 IGCIQVAIKPLFKTGLDVPIYVALRDKRHLNFSSSLLGIVQSNLDNGPVYFTCKPGFTVA 180

Query: 289 LQDKNIFDVLCLDIRGKGLRLKDGSYPFVILFRMYYKLMHTNISPNALGVSPRNYTMLME 323
           LQDKNI+DVLCLD+R KGLRLK+GSYPF +++ +YYK+M+TN SP ALGVSP+NYTMLM+
Sbjct: 181 LQDKNIYDVLCLDVRAKGLRLKNGSYPFSVMYILYYKMMYTNASPKALGVSPKNYTMLMK 240

BLAST of Lag0032274 vs. NCBI nr
Match: KAA0059217.1 (Enzymatic polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 202.6 bits (514), Expect = 5.2e-48
Identity = 117/280 (41.79%), Postives = 155/280 (55.36%), Query Frame = 0

Query: 109 MLSKFKKKFS-FGPTIIDATTSSSSSSKNPK--PNAKELEKALSRHKRIESQISQVEDKL 168
           MLSK   K S   P IIDAT SSS SS +P    +  +L  AL+ H   E+ ++QVE++L
Sbjct: 1   MLSKLLSKLSPHSPAIIDATASSSYSSSSPSNLRSKNDLSTALAEHNSAEAHLAQVENRL 60

Query: 169 QIWSIPKLEVNTIYKISPFNFLETDVIRVSEDNIPMEDDFTEYQLLINEDI--------- 228
           + WSIPKLE N +YKI+ FNF + DVI ++E+N+ M+D+FT  +LL  E +         
Sbjct: 61  KNWSIPKLEANQVYKINTFNFSQQDVIVITEENVAMKDEFTAIKLLPEETLFKVRDRFKY 120

Query: 229 -------------------------------------------------------DPDFT 288
                                                                   P  T
Sbjct: 121 LHIGCVQVALKPLFREGLDVPVYLALRDKRHLRFTPSLLGIVQSNLEQGPVYFNCRPGLT 180

Query: 289 VALQDKNIFDVLCLDIRGKGLRLKDGSYPFVILFRMYYKLMHTNISPNALGVSPRNYTML 322
           V+LQDKNI D + LD+  +GL LKDGS PF + +R+Y+KLMHTN+SP ALG+SP+ YTML
Sbjct: 181 VSLQDKNIMDTISLDVHSQGLELKDGSLPFAVSYRIYFKLMHTNLSPKALGISPKGYTML 240

BLAST of Lag0032274 vs. NCBI nr
Match: TYJ97599.1 (Enzymatic polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 202.6 bits (514), Expect = 5.2e-48
Identity = 117/280 (41.79%), Postives = 155/280 (55.36%), Query Frame = 0

Query: 109 MLSKFKKKFS-FGPTIIDATTSSSSSSKNPK--PNAKELEKALSRHKRIESQISQVEDKL 168
           MLSK   K S   P IIDAT SSS SS +P    +  +L  AL+ H   E+ ++QVE++L
Sbjct: 1   MLSKLLSKLSPHSPAIIDATASSSYSSSSPSNLRSKNDLSTALAEHNSAEAHLAQVENRL 60

Query: 169 QIWSIPKLEVNTIYKISPFNFLETDVIRVSEDNIPMEDDFTEYQLLINEDI--------- 228
           + WSIPKLE N +YKI+ FNF + DVI ++E+N+ M+D+FT  +LL  E +         
Sbjct: 61  KNWSIPKLEANQVYKINTFNFSQQDVIVITEENVAMKDEFTAIKLLPEETLFKVRDRFKY 120

Query: 229 -------------------------------------------------------DPDFT 288
                                                                   P  T
Sbjct: 121 LHIGCVQVALKPLFREGLDVPVYLALRDKRHLRFTPSLLGIVQSNLEQGPVYFNCRPGLT 180

Query: 289 VALQDKNIFDVLCLDIRGKGLRLKDGSYPFVILFRMYYKLMHTNISPNALGVSPRNYTML 322
           V+LQDKNI D + LD+  +GL LKDGS PF + +R+Y+KLMHTN+SP ALG+SP+ YTML
Sbjct: 181 VSLQDKNIMDTISLDVHSQGLELKDGSLPFAVSYRIYFKLMHTNLSPKALGISPKGYTML 240

BLAST of Lag0032274 vs. NCBI nr
Match: KAA0041674.1 (movement protein [Cucumis melo var. makuwa] >TYK06295.1 movement protein [Cucumis melo var. makuwa])

HSP 1 Score: 202.6 bits (514), Expect = 5.2e-48
Identity = 118/280 (42.14%), Postives = 158/280 (56.43%), Query Frame = 0

Query: 109 MLSKFKKKFS-FGPTIIDATTSS--SSSSKNPKPNAKELEKALSRHKRIESQISQVEDKL 168
           MLSK   K S   PTIIDAT SS  SSSS N +P   +L  AL++H  +E+ ++QVE++L
Sbjct: 1   MLSKLLSKLSPHSPTIIDATVSSPYSSSSSNLRPQ-DDLSTALAKHNTVEAHLAQVENRL 60

Query: 169 QIWSIPKLEVNTIYKISPFNFLETDVIRVSEDNIPMEDDFTEYQLLINEDI--------- 228
           + WSIPKLE N +YKI+ FNF + DVI ++E+N+ M+++FT  +LL  E +         
Sbjct: 61  KNWSIPKLEANQVYKINTFNFSQQDVIVITEENVAMKNEFTAIKLLPEETLFKVKEKFKY 120

Query: 229 -------------------------------------------------------DPDFT 288
                                                                   PD T
Sbjct: 121 LHIGCVQVALKPLFREGLDVPVYLALRDKRHLRFTPSLLGIVQSNLEQGPVYFNCRPDLT 180

Query: 289 VALQDKNIFDVLCLDIRGKGLRLKDGSYPFVILFRMYYKLMHTNISPNALGVSPRNYTML 322
           V+LQDKNI D + LD+  + L LKDGS PF + +R+Y+KLMHTN+SP ALG+SP+ YTML
Sbjct: 181 VSLQDKNIMDTISLDVHSQRLELKDGSLPFAVSYRIYFKLMHTNLSPKALGISPKGYTML 240

BLAST of Lag0032274 vs. NCBI nr
Match: KAA0056776.1 (Enzymatic polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 202.6 bits (514), Expect = 5.2e-48
Identity = 117/280 (41.79%), Postives = 155/280 (55.36%), Query Frame = 0

Query: 109 MLSKFKKKFS-FGPTIIDATTSSSSSSKNPK--PNAKELEKALSRHKRIESQISQVEDKL 168
           MLSK   K S   P IIDAT SSS SS +P    +  +L  AL+ H   E+ ++QVE++L
Sbjct: 1   MLSKLLSKLSPHSPAIIDATASSSYSSSSPSNLRSKNDLSTALAEHNSAEAHLAQVENRL 60

Query: 169 QIWSIPKLEVNTIYKISPFNFLETDVIRVSEDNIPMEDDFTEYQLLINEDI--------- 228
           + WSIPKLE N +YKI+ FNF + DVI ++E+N+ M+D+FT  +LL  E +         
Sbjct: 61  KNWSIPKLEANQVYKINTFNFSQQDVIVITEENVAMKDEFTAIKLLPEETLFKVRDRFKY 120

Query: 229 -------------------------------------------------------DPDFT 288
                                                                   P  T
Sbjct: 121 LHIGCVQVALKPLFREGLDVPVYLALRDKRHLRFTPSLLGIVQSNLEQGPVYFNCRPGLT 180

Query: 289 VALQDKNIFDVLCLDIRGKGLRLKDGSYPFVILFRMYYKLMHTNISPNALGVSPRNYTML 322
           V+LQDKNI D + LD+  +GL LKDGS PF + +R+Y+KLMHTN+SP ALG+SP+ YTML
Sbjct: 181 VSLQDKNIMDTISLDVHSQGLELKDGSLPFAVSYRIYFKLMHTNLSPKALGISPKGYTML 240

BLAST of Lag0032274 vs. ExPASy Swiss-Prot
Match: Q6YPG5 (Putative nitric oxide synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0104700 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-28
Identity = 58/104 (55.77%), Postives = 77/104 (74.04%), Query Frame = 0

Query: 2   DDWRGLETVRQLKINFEEEQRPACDVAISGLGWIAVE----QKESLQNTHSDLESTVDEL 61
           + W GL+ VR+L+I +EE  RPACD+AISGLGW+AVE       +   +  + ++   EL
Sbjct: 442 EGWVGLQGVRELQIKYEESDRPACDIAISGLGWVAVEPLGVPSSNPDESAEEEDNESGEL 501

Query: 62  ELAVHVPKPVEIFVRPSMPVGKAGAEWYQYRDLTEEEEEARPRW 102
            L VHVPKPVEIFVRP +PVGKA ++WY+Y++LTEEEEE RP+W
Sbjct: 502 HLRVHVPKPVEIFVRPPLPVGKAASQWYRYQELTEEEEELRPKW 545

BLAST of Lag0032274 vs. ExPASy Swiss-Prot
Match: Q66GP9 (NO-associated protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NOA1 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 8.9e-27
Identity = 52/103 (50.49%), Postives = 75/103 (72.82%), Query Frame = 0

Query: 1   MDDWRGLETVRQLKINFEEEQRPACDVAISGLGWIAVEQKESLQNTH-SDLESTVDELEL 60
           M +W+GL++ R L+I   + +RPA DVAISGLGWI++E     + T   DL     E+ +
Sbjct: 458 MQEWKGLQSHRLLQIEINDAKRPASDVAISGLGWISIEPIRKTRGTEPRDLNEAEHEIHI 517

Query: 61  AVHVPKPVEIFVRPSMPVGKAGAEWYQYRDLTEEEEEARPRWF 103
            V VPKPVE+F+RP++P+G +G EWYQYR+LT++EEE RP+W+
Sbjct: 518 CVSVPKPVEVFLRPTLPIGTSGTEWYQYRELTDKEEEVRPKWY 560

BLAST of Lag0032274 vs. ExPASy TrEMBL
Match: A0A5A7SRU6 (Polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G00880 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 1.5e-56
Identity = 127/279 (45.52%), Postives = 170/279 (60.93%), Query Frame = 0

Query: 109 MLSKFKKKFSFGPTIIDATTSSSSSSKNPKPNAKELEKALSRHKRIESQISQVEDKLQIW 168
           MLSK KKK S    +I+   +SSSSS N   N++ LE+ LS++ R+E+Q S +E++LQ W
Sbjct: 1   MLSKLKKKLSLNSLVIE---TSSSSSNNFGINSENLEETLSKYSRVENQSSSIENRLQNW 60

Query: 169 SIPKLEVNTIYKISPFNFLETDVIRVSEDNIPMEDDFTEYQLLINEDID----------- 228
           SIP+LE   IY    FNF E DV+R+SEDNIP+ +DF++Y+LL NE+I+           
Sbjct: 61  SIPRLEPKEIYAHPFFNFREKDVVRISEDNIPVSEDFSKYRLLANENIELCRKHNYRHLH 120

Query: 229 ------------------------------------------------------PDFTVA 288
                                                                 P FTVA
Sbjct: 121 IGCIQVAIKPLFKTGLDVPIYVALRDKRHLNFSSSLLGIVQSNLDNGPVYFTCKPGFTVA 180

Query: 289 LQDKNIFDVLCLDIRGKGLRLKDGSYPFVILFRMYYKLMHTNISPNALGVSPRNYTMLME 323
           LQDKNI+DVLCLD+R KGLRLK+GSYPF +++ +YYK+M+TN SP ALGVSP+NYTMLM+
Sbjct: 181 LQDKNIYDVLCLDVRAKGLRLKNGSYPFSVMYILYYKMMYTNASPKALGVSPKNYTMLMK 240

BLAST of Lag0032274 vs. ExPASy TrEMBL
Match: A0A5D3C4I7 (Movement protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold594G00050 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 2.5e-48
Identity = 118/280 (42.14%), Postives = 158/280 (56.43%), Query Frame = 0

Query: 109 MLSKFKKKFS-FGPTIIDATTSS--SSSSKNPKPNAKELEKALSRHKRIESQISQVEDKL 168
           MLSK   K S   PTIIDAT SS  SSSS N +P   +L  AL++H  +E+ ++QVE++L
Sbjct: 1   MLSKLLSKLSPHSPTIIDATVSSPYSSSSSNLRPQ-DDLSTALAKHNTVEAHLAQVENRL 60

Query: 169 QIWSIPKLEVNTIYKISPFNFLETDVIRVSEDNIPMEDDFTEYQLLINEDI--------- 228
           + WSIPKLE N +YKI+ FNF + DVI ++E+N+ M+++FT  +LL  E +         
Sbjct: 61  KNWSIPKLEANQVYKINTFNFSQQDVIVITEENVAMKNEFTAIKLLPEETLFKVKEKFKY 120

Query: 229 -------------------------------------------------------DPDFT 288
                                                                   PD T
Sbjct: 121 LHIGCVQVALKPLFREGLDVPVYLALRDKRHLRFTPSLLGIVQSNLEQGPVYFNCRPDLT 180

Query: 289 VALQDKNIFDVLCLDIRGKGLRLKDGSYPFVILFRMYYKLMHTNISPNALGVSPRNYTML 322
           V+LQDKNI D + LD+  + L LKDGS PF + +R+Y+KLMHTN+SP ALG+SP+ YTML
Sbjct: 181 VSLQDKNIMDTISLDVHSQRLELKDGSLPFAVSYRIYFKLMHTNLSPKALGISPKGYTML 240

BLAST of Lag0032274 vs. ExPASy TrEMBL
Match: A0A5D3BEY3 (Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold690G00300 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 2.5e-48
Identity = 117/280 (41.79%), Postives = 155/280 (55.36%), Query Frame = 0

Query: 109 MLSKFKKKFS-FGPTIIDATTSSSSSSKNPK--PNAKELEKALSRHKRIESQISQVEDKL 168
           MLSK   K S   P IIDAT SSS SS +P    +  +L  AL+ H   E+ ++QVE++L
Sbjct: 1   MLSKLLSKLSPHSPAIIDATASSSYSSSSPSNLRSKNDLSTALAEHNSAEAHLAQVENRL 60

Query: 169 QIWSIPKLEVNTIYKISPFNFLETDVIRVSEDNIPMEDDFTEYQLLINEDI--------- 228
           + WSIPKLE N +YKI+ FNF + DVI ++E+N+ M+D+FT  +LL  E +         
Sbjct: 61  KNWSIPKLEANQVYKINTFNFSQQDVIVITEENVAMKDEFTAIKLLPEETLFKVRDRFKY 120

Query: 229 -------------------------------------------------------DPDFT 288
                                                                   P  T
Sbjct: 121 LHIGCVQVALKPLFREGLDVPVYLALRDKRHLRFTPSLLGIVQSNLEQGPVYFNCRPGLT 180

Query: 289 VALQDKNIFDVLCLDIRGKGLRLKDGSYPFVILFRMYYKLMHTNISPNALGVSPRNYTML 322
           V+LQDKNI D + LD+  +GL LKDGS PF + +R+Y+KLMHTN+SP ALG+SP+ YTML
Sbjct: 181 VSLQDKNIMDTISLDVHSQGLELKDGSLPFAVSYRIYFKLMHTNLSPKALGISPKGYTML 240

BLAST of Lag0032274 vs. ExPASy TrEMBL
Match: A0A5A7UX67 (Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G001460 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 2.5e-48
Identity = 117/280 (41.79%), Postives = 155/280 (55.36%), Query Frame = 0

Query: 109 MLSKFKKKFS-FGPTIIDATTSSSSSSKNPK--PNAKELEKALSRHKRIESQISQVEDKL 168
           MLSK   K S   P IIDAT SSS SS +P    +  +L  AL+ H   E+ ++QVE++L
Sbjct: 1   MLSKLLSKLSPHSPAIIDATASSSYSSSSPSNLRSKNDLSTALAEHNSAEAHLAQVENRL 60

Query: 169 QIWSIPKLEVNTIYKISPFNFLETDVIRVSEDNIPMEDDFTEYQLLINEDI--------- 228
           + WSIPKLE N +YKI+ FNF + DVI ++E+N+ M+D+FT  +LL  E +         
Sbjct: 61  KNWSIPKLEANQVYKINTFNFSQQDVIVITEENVAMKDEFTAIKLLPEETLFKVRDRFKY 120

Query: 229 -------------------------------------------------------DPDFT 288
                                                                   P  T
Sbjct: 121 LHIGCVQVALKPLFREGLDVPVYLALRDKRHLRFTPSLLGIVQSNLEQGPVYFNCRPGLT 180

Query: 289 VALQDKNIFDVLCLDIRGKGLRLKDGSYPFVILFRMYYKLMHTNISPNALGVSPRNYTML 322
           V+LQDKNI D + LD+  +GL LKDGS PF + +R+Y+KLMHTN+SP ALG+SP+ YTML
Sbjct: 181 VSLQDKNIMDTISLDVHSQGLELKDGSLPFAVSYRIYFKLMHTNLSPKALGISPKGYTML 240

BLAST of Lag0032274 vs. ExPASy TrEMBL
Match: A0A5A7UR29 (Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold486G00660 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 2.5e-48
Identity = 117/280 (41.79%), Postives = 155/280 (55.36%), Query Frame = 0

Query: 109 MLSKFKKKFS-FGPTIIDATTSSSSSSKNPK--PNAKELEKALSRHKRIESQISQVEDKL 168
           MLSK   K S   P IIDAT SSS SS +P    +  +L  AL+ H   E+ ++QVE++L
Sbjct: 1   MLSKLLSKLSPHSPAIIDATASSSYSSSSPSNLRSKNDLSTALAEHNSAEAHLAQVENRL 60

Query: 169 QIWSIPKLEVNTIYKISPFNFLETDVIRVSEDNIPMEDDFTEYQLLINEDI--------- 228
           + WSIPKLE N +YKI+ FNF + DVI ++E+N+ M+D+FT  +LL  E +         
Sbjct: 61  KNWSIPKLEANQVYKINTFNFSQQDVIVITEENVAMKDEFTAIKLLPEETLFKVRDRFKY 120

Query: 229 -------------------------------------------------------DPDFT 288
                                                                   P  T
Sbjct: 121 LHIGCVQVALKPLFREGLDVPVYLALRDKRHLRFTPSLLGIVQSNLEQGPVYFNCRPGLT 180

Query: 289 VALQDKNIFDVLCLDIRGKGLRLKDGSYPFVILFRMYYKLMHTNISPNALGVSPRNYTML 322
           V+LQDKNI D + LD+  +GL LKDGS PF + +R+Y+KLMHTN+SP ALG+SP+ YTML
Sbjct: 181 VSLQDKNIMDTISLDVHSQGLELKDGSLPFAVSYRIYFKLMHTNLSPKALGISPKGYTML 240

BLAST of Lag0032274 vs. TAIR 10
Match: AT3G47450.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 6.3e-28
Identity = 52/103 (50.49%), Postives = 75/103 (72.82%), Query Frame = 0

Query: 1   MDDWRGLETVRQLKINFEEEQRPACDVAISGLGWIAVEQKESLQNTH-SDLESTVDELEL 60
           M +W+GL++ R L+I   + +RPA DVAISGLGWI++E     + T   DL     E+ +
Sbjct: 458 MQEWKGLQSHRLLQIEINDAKRPASDVAISGLGWISIEPIRKTRGTEPRDLNEAEHEIHI 517

Query: 61  AVHVPKPVEIFVRPSMPVGKAGAEWYQYRDLTEEEEEARPRWF 103
            V VPKPVE+F+RP++P+G +G EWYQYR+LT++EEE RP+W+
Sbjct: 518 CVSVPKPVEVFLRPTLPIGTSGTEWYQYRELTDKEEEVRPKWY 560

BLAST of Lag0032274 vs. TAIR 10
Match: AT3G47450.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 6.3e-28
Identity = 52/103 (50.49%), Postives = 75/103 (72.82%), Query Frame = 0

Query: 1   MDDWRGLETVRQLKINFEEEQRPACDVAISGLGWIAVEQKESLQNTH-SDLESTVDELEL 60
           M +W+GL++ R L+I   + +RPA DVAISGLGWI++E     + T   DL     E+ +
Sbjct: 458 MQEWKGLQSHRLLQIEINDAKRPASDVAISGLGWISIEPIRKTRGTEPRDLNEAEHEIHI 517

Query: 61  AVHVPKPVEIFVRPSMPVGKAGAEWYQYRDLTEEEEEARPRWF 103
            V VPKPVE+F+RP++P+G +G EWYQYR+LT++EEE RP+W+
Sbjct: 518 CVSVPKPVEVFLRPTLPIGTSGTEWYQYRELTDKEEEVRPKWY 560

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0033914.13.0e-5645.52polyprotein [Cucumis melo var. makuwa] >TYK01296.1 polyprotein [Cucumis melo var... [more]
KAA0059217.15.2e-4841.79Enzymatic polyprotein [Cucumis melo var. makuwa][more]
TYJ97599.15.2e-4841.79Enzymatic polyprotein [Cucumis melo var. makuwa][more]
KAA0041674.15.2e-4842.14movement protein [Cucumis melo var. makuwa] >TYK06295.1 movement protein [Cucumi... [more]
KAA0056776.15.2e-4841.79Enzymatic polyprotein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q6YPG51.6e-2855.77Putative nitric oxide synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g... [more]
Q66GP98.9e-2750.49NO-associated protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A5A7SRU61.5e-5645.52Polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G00880 PE=... [more]
A0A5D3C4I72.5e-4842.14Movement protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold594G000... [more]
A0A5D3BEY32.5e-4841.79Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold69... [more]
A0A5A7UX672.5e-4841.79Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43... [more]
A0A5A7UR292.5e-4841.79Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold48... [more]
Match NameE-valueIdentityDescription
AT3G47450.16.3e-2850.49P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G47450.26.3e-2850.49P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 40..60
NoneNo IPR availableCOILSCoilCoilcoord: 144..164
IPR044229NO-associated protein 1PANTHERPTHR47569NO-ASSOCIATED PROTEIN 1, CHLOROPLASTIC/MITOCHONDRIALcoord: 2..102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032274.1Lag0032274.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding