Lag0032201 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032201
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCDGSH iron-sulfur domain-containing protein NEET
Locationchr11: 27152319 .. 27152783 (-)
RNA-Seq ExpressionLag0032201
SyntenyLag0032201
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCCACTGTGGCCACTTCTTCCTTATCAATAATCCATCCTCCTCGTCGCAACGTCAACGGCGGCGGACTGCGGCGACTGGTGGTGGTCAGAGCTGAAGGCGGCGAACCCATAAACCCTGCAATCAGGAAGAGCGAAGACAAAGTGGTGGACTCTGTGCTTGTCGCTGAACTGGCCAAGCCCCTCACTGCTTATTGCAGGTTCCTTTCTTTTTCTCCTTTTATCAACCAACCAGGGACAAAACTGAAAAATGTTATTAGACAAGGGACTAAATTGAAACTTTGAAATACACCGAAGAATTAGTTCAATCGATGTGCATGTATGTTTGAGATTTGAGTTTAGGATATTTATGGATGTAGATGTTGGAGGTCAGGCACATTTCCTCTGTGTGATGGAAGCCATGTGAAGCACAACAAGGCCACTGGAGATAATGTTGGTCCTTTGCTCTTAAAGAAGCAGTAG

mRNA sequence

ATGGCGTCCACTGTGGCCACTTCTTCCTTATCAATAATCCATCCTCCTCGTCGCAACGTCAACGGCGGCGGACTGCGGCGACTGGTGGTGGTCAGAGCTGAAGGCGGCGAACCCATAAACCCTGCAATCAGGAAGAGCGAAGACAAAGTGGTGGACTCTGTGCTTGTCGCTGAACTGGCCAAGCCCCTCACTGCTTATTGCAGATGTTGGAGGTCAGGCACATTTCCTCTGTGTGATGGAAGCCATGTGAAGCACAACAAGGCCACTGGAGATAATGTTGGTCCTTTGCTCTTAAAGAAGCAGTAG

Coding sequence (CDS)

ATGGCGTCCACTGTGGCCACTTCTTCCTTATCAATAATCCATCCTCCTCGTCGCAACGTCAACGGCGGCGGACTGCGGCGACTGGTGGTGGTCAGAGCTGAAGGCGGCGAACCCATAAACCCTGCAATCAGGAAGAGCGAAGACAAAGTGGTGGACTCTGTGCTTGTCGCTGAACTGGCCAAGCCCCTCACTGCTTATTGCAGATGTTGGAGGTCAGGCACATTTCCTCTGTGTGATGGAAGCCATGTGAAGCACAACAAGGCCACTGGAGATAATGTTGGTCCTTTGCTCTTAAAGAAGCAGTAG

Protein sequence

MASTVATSSLSIIHPPRRNVNGGGLRRLVVVRAEGGEPINPAIRKSEDKVVDSVLVAELAKPLTAYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLLKKQ
Homology
[Close]
BLAST of Lag0032201 vs. NCBI nr
Match: XP_022996357.1 (CDGSH iron-sulfur domain-containing protein NEET [Cucurbita maxima])

HSP 1 Score: 166.4 bits (420), Expect = 1.3e-37
Identity = 82/103 (79.61%), Postives = 87/103 (84.47%), Query Frame = 0 

Query: 1   MASTVATSSLSIIHPPRRNVNGGGL--RRLVVVRAEGGEPINPAIRKSEDKVVDSVLVAE 60
           MAS  ATS+L    PP  N   GG+  RR+VVVRAEGGE INPAIRKSEDKVVDSV+VA+
Sbjct: 1   MASLAATSALPTTRPPHGNFTVGGMPRRRMVVVRAEGGEAINPAIRKSEDKVVDSVVVAD 60

Query: 61  LAKPLTAYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLLKKQ 102
           LAKPLT YCRCWRS TFPLCDGSHVKHNKATGDNVGPLLLKKQ
Sbjct: 61  LAKPLTPYCRCWRSATFPLCDGSHVKHNKATGDNVGPLLLKKQ 103

[Close]
BLAST of Lag0032201 vs. NCBI nr
Match: XP_023534172.1 (CDGSH iron-sulfur domain-containing protein NEET [Cucurbita pepo subsp. pepo])

HSP 1 Score: 164.9 bits (416), Expect = 3.7e-37
Identity = 82/103 (79.61%), Postives = 87/103 (84.47%), Query Frame = 0 

Query: 1   MASTVATSSLSIIHPPRRNVNGGGL--RRLVVVRAEGGEPINPAIRKSEDKVVDSVLVAE 60
           MAS  ATS+L    PP  N   GG+  RR+VVVRAEGGE INPAIRKSEDKVVDSV+VA+
Sbjct: 1   MASLPATSALPTTRPPHVNFTLGGMPRRRMVVVRAEGGEAINPAIRKSEDKVVDSVVVAD 60

Query: 61  LAKPLTAYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLLKKQ 102
           LAKPLT YCRCWRS TFPLCDGSHVKHNKATGDNVGPLLLKKQ
Sbjct: 61  LAKPLTPYCRCWRSATFPLCDGSHVKHNKATGDNVGPLLLKKQ 103

[Close]
BLAST of Lag0032201 vs. NCBI nr
Match: KAG6605678.1 (CDGSH iron-sulfur domain-containing protein NEET, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 161.8 bits (408), Expect = 3.2e-36
Identity = 80/104 (76.92%), Postives = 86/104 (82.69%), Query Frame = 0 

Query: 1   MASTVATSSLSIIHPPRRNVNGGGL---RRLVVVRAEGGEPINPAIRKSEDKVVDSVLVA 60
           MAS  ATS+L    PP  N   GG+   R +VVVRAEGG+ INPAIRKSEDKVVDSV+VA
Sbjct: 1   MASLPATSALPTTRPPHSNFTVGGMPRRRMVVVVRAEGGQAINPAIRKSEDKVVDSVVVA 60

Query: 61  ELAKPLTAYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLLKKQ 102
           +LAKPLT YCRCWRS TFPLCDGSHVKHNKATGDNVGPLLLKKQ
Sbjct: 61  DLAKPLTPYCRCWRSATFPLCDGSHVKHNKATGDNVGPLLLKKQ 104

[Close]
BLAST of Lag0032201 vs. NCBI nr
Match: XP_022958373.1 (CDGSH iron-sulfur domain-containing protein NEET [Cucurbita moschata])

HSP 1 Score: 161.4 bits (407), Expect = 4.1e-36
Identity = 80/104 (76.92%), Postives = 86/104 (82.69%), Query Frame = 0 

Query: 1   MASTVATSSLSIIHPPRRNVNGGGL---RRLVVVRAEGGEPINPAIRKSEDKVVDSVLVA 60
           MAS  ATS+L    PP  N   GG+   R +VVVRAEGG+ INPAIRKSEDKVVDSV+VA
Sbjct: 1   MASLPATSALPTTRPPHGNFTVGGMPRRRMVVVVRAEGGQAINPAIRKSEDKVVDSVVVA 60

Query: 61  ELAKPLTAYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLLKKQ 102
           +LAKPLT YCRCWRS TFPLCDGSHVKHNKATGDNVGPLLLKKQ
Sbjct: 61  DLAKPLTPYCRCWRSATFPLCDGSHVKHNKATGDNVGPLLLKKQ 104

[Close]
BLAST of Lag0032201 vs. NCBI nr
Match: KAG7035586.1 (CDGSH iron-sulfur domain-containing protein NEET [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 160.2 bits (404), Expect = 9.2e-36
Identity = 80/104 (76.92%), Postives = 86/104 (82.69%), Query Frame = 0 

Query: 1   MASTVATSSLSIIHPPRRNVNGGGL---RRLVVVRAEGGEPINPAIRKSEDKVVDSVLVA 60
           MAS  ATS+L    PP  N   GG+   R +VVVRAEGG+ INPAIRKSEDKVVDSV+VA
Sbjct: 1   MASLPATSALPTTPPPHVNFTVGGIPRRRMVVVVRAEGGQAINPAIRKSEDKVVDSVVVA 60

Query: 61  ELAKPLTAYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLLKKQ 102
           +LAKPLT YCRCWRS TFPLCDGSHVKHNKATGDNVGPLLLKKQ
Sbjct: 61  DLAKPLTPYCRCWRSATFPLCDGSHVKHNKATGDNVGPLLLKKQ 104

[Close]
BLAST of Lag0032201 vs. ExPASy Swiss-Prot
Match: Q9FLI7 (CDGSH iron-sulfur domain-containing protein NEET OS=Arabidopsis thaliana OX=3702 GN=NEET PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.3e-32
Identity = 71/105 (67.62%), Postives = 80/105 (76.19%), Query Frame = 0 

Query: 1   MASTVATSSLSIIHPPRRNVNGGGL----RRLVVVRAEGGEPINPAIRKSEDKVVDSVLV 60
           +AST  T        P + V GG +    +R+VVVRAEGG  INP IRK+EDKVVDSV+V
Sbjct: 4   IASTFGTGLSYAGELPFKPVTGGEVGRKQQRMVVVRAEGGGGINPEIRKNEDKVVDSVVV 63

Query: 61  AELAKPLTAYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLLKKQ 102
            EL+K +T YCRCWRSGTFPLCDGSHVKHNKA GDNVGPLLLKKQ
Sbjct: 64  TELSKNITPYCRCWRSGTFPLCDGSHVKHNKANGDNVGPLLLKKQ 108

[Close]
BLAST of Lag0032201 vs. ExPASy Swiss-Prot
Match: Q7T326 (CDGSH iron-sulfur domain-containing protein 2 OS=Danio rerio OX=7955 GN=cisd2 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.4e-17
Identity = 37/63 (58.73%), Postives = 47/63 (74.60%), Query Frame = 0 

Query: 39  INPAIRKSEDKVVDSVLVAELAKPLTAYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLL 98
           IN  I+K   KVV+ + + +L  P   YCRCWRS TFP+CD SH+KHN+ TGDNVGPL+L
Sbjct: 71  INLKIQKENPKVVNEIDIEDLRTPNVCYCRCWRSKTFPVCDKSHIKHNELTGDNVGPLIL 130

Query: 99  KKQ 102
           KK+
Sbjct: 131 KKK 133

[Close]
BLAST of Lag0032201 vs. ExPASy Swiss-Prot
Match: Q6PCF8 (CDGSH iron-sulfur domain-containing protein 2A OS=Xenopus laevis OX=8355 GN=cisd2-a PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 5.8e-17
Identity = 38/63 (60.32%), Postives = 47/63 (74.60%), Query Frame = 0 

Query: 39  INPAIRKSEDKVVDSVLVAELAKPLTAYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLL 98
           IN  I+K   KVV+ + + +L     AYCRCWRS TFP+CDGSH KHN+ TGDNVGPL+L
Sbjct: 71  INLKIQKENPKVVNEINIEDLHLAKAAYCRCWRSKTFPVCDGSHNKHNELTGDNVGPLIL 130

Query: 99  KKQ 102
           KK+
Sbjct: 131 KKK 133

[Close]
BLAST of Lag0032201 vs. ExPASy Swiss-Prot
Match: Q6AZG1 (CDGSH iron-sulfur domain-containing protein 2B OS=Xenopus laevis OX=8355 GN=cisd2-b PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 5.8e-17
Identity = 38/63 (60.32%), Postives = 47/63 (74.60%), Query Frame = 0 

Query: 39  INPAIRKSEDKVVDSVLVAELAKPLTAYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLL 98
           IN  I+K   KVV+ + + +L     AYCRCWRS TFP+CDGSH KHN+ TGDNVGPL+L
Sbjct: 71  INLKIQKENPKVVNEINIEDLHLAKAAYCRCWRSKTFPVCDGSHNKHNELTGDNVGPLIL 130

Query: 99  KKQ 102
           KK+
Sbjct: 131 KKK 133

[Close]
BLAST of Lag0032201 vs. ExPASy Swiss-Prot
Match: Q05B71 (CDGSH iron-sulfur domain-containing protein 2 OS=Bos taurus OX=9913 GN=CISD2 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 5.8e-17
Identity = 38/63 (60.32%), Postives = 46/63 (73.02%), Query Frame = 0 

Query: 39  INPAIRKSEDKVVDSVLVAELAKPLTAYCRCWRSGTFPLCDGSHVKHNKATGDNVGPLLL 98
           IN  I+K   KVV+ + + +L     AYCRCWRS TFP CDGSH KHN+ TGDNVGPL+L
Sbjct: 71  INLKIQKENPKVVNEINIEDLCLTKAAYCRCWRSKTFPACDGSHNKHNELTGDNVGPLIL 130

Query: 99  KKQ 102
           KK+
Sbjct: 131 KKK 133

The following BLAST results are available for this feature:
BLAST of Lag0032201 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022996357.11.3e-3779.61CDGSH iron-sulfur domain-containing protein NEET [Cucurbita maxima][more]
XP_023534172.13.7e-3779.61CDGSH iron-sulfur domain-containing protein NEET [Cucurbita pepo subsp. pepo][more]
KAG6605678.13.2e-3676.92CDGSH iron-sulfur domain-containing protein NEET, partial [Cucurbita argyrosperm... [more]
XP_022958373.14.1e-3676.92CDGSH iron-sulfur domain-containing protein NEET [Cucurbita moschata][more]
KAG7035586.19.2e-3676.92CDGSH iron-sulfur domain-containing protein NEET [Cucurbita argyrosperma subsp. ... [more]
BLAST of Lag0032201 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FLI71.3e-3267.62CDGSH iron-sulfur domain-containing protein NEET OS=Arabidopsis thaliana OX=3702... [more]
Q7T3263.4e-1758.73CDGSH iron-sulfur domain-containing protein 2 OS=Danio rerio OX=7955 GN=cisd2 PE... [more]
Q6PCF85.8e-1760.32CDGSH iron-sulfur domain-containing protein 2A OS=Xenopus laevis OX=8355 GN=cisd... [more]
Q6AZG15.8e-1760.32CDGSH iron-sulfur domain-containing protein 2B OS=Xenopus laevis OX=8355 GN=cisd... [more]
Q05B715.8e-1760.32CDGSH iron-sulfur domain-containing protein 2 OS=Bos taurus OX=9913 GN=CISD2 PE=... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018967Iron-binding zinc finger, CDGSH typeSMARTSM00704znf_cdgshcoord: 49..88
e-value: 5.8E-9
score: 45.8
IPR018967Iron-binding zinc finger, CDGSH typePFAMPF09360zf-CDGSHcoord: 51..82
e-value: 7.0E-10
score: 39.0
IPR042216MitoNEET, CDGSH iron-sulfur domainGENE3D3.40.5.90coord: 55..101
e-value: 2.7E-24
score: 86.3
NoneNo IPR availablePANTHERPTHR13680:SF5CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 1coord: 25..101
IPR045131CDGSH iron-sulfur domain-containing protein 1/2PANTHERPTHR13680CDGSH IRON-SULFUR DOMAIN-CONTAINING PROTEIN 1coord: 25..101

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032201.1Lag0032201.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010506 regulation of autophagy
cellular_component GO:0005741 mitochondrial outer membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0046872 metal ion binding