Lag0032171 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0032171
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Tryptophanyl-tRNA synthetase
Location	chr11: 26548141 .. 26550091 (-)
RNA-Seq Expression	Lag0032171
Synteny	Lag0032171

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGGCGACACGAGACATCCTACGACCTTCACTCGCGAACCTCTGGTTCGGCTTCACTCTCTGTTTCTCGCGCGAACTACAATACAAGACACTCGCGAACCTCCGACTTCACTCTCTGTTCTCTGTCTCTTCTCCCTCCGGTCATCTTCTCCCTCCGGTCATCTTCTCCGGCGTCGACCAAGAACAAGGAGCGGCGGTTTCGCGATAGCAGTCTTCATCTCCGGCGTTCTTCTCCAGCAGTGATGACGTTTTGGGCAGTCTGACAGTGTTCCTGCAAGTGCAGCAGCGGTTGGGTTGTCTTGGTGAGTTTGGATTCGTTTCTCTCAATTGTTATTGGTCTTCATACTCGATCTCTTGCGCTTTATTGTTCTCAATATCTCACTCCCGCTTTTTGTTGGTTATCTTGCGGGTTTAAGGTGCGGATGGAAGTGAGATTTGATTTTGATGTTGATATTTGGAAAATAATTATTGGTGTTGTTCTTCAACAATTTTGGCTATTCTGGATTTTGTGGAGTACTACATTGCCTAATTTTCTACTAGGCTAATCACAAAGGTTAGGAGAATGAAAGTCATTGTTTGATTTTTTTTCGTTTCGGCTTTTTTTTGTTTGGTATTTTTCTTGGGTTTGAATTATCAATATGCATTATGGTGGTATATGTGTTTAGAGGGGAAAACTTGACTTACCATATGACCAACAAGACAGACCCTTAATTTTCAATGTTTGATTACAAAAGTTTAAAATTTAGAATGAAACTCAATCATAGACTAATCCCACACTTATAAATATCACCTTCAAATCTTCATTATCCAAAAACACAATTGGGTTTTAGAAGCTTTTTGGTTTCAAAGATTAAACTTTCAGAGCTTAAAGCCTTCATCTTTTCCTCTAATGGAAATGGTTCCAACCAAAATCCAAGTCTTCTCCATTACCCAAATGTCAATTTTCTTGACAACATTTCTGATTTGTTCAATGACAGTTTCTTTGGCAATCCAGCTAATCTTCCCAGATTCCTGGAAAATTGGGGAGAAATTTTCCCAGCAATTTTCTCTCAAGCCAATCAGAACACTGCACCTGTTTACTTTTTGGTGTTTTTTTGGTATTTTTCTTGGGTTTGAATGATAAAATCTCTCTGTGGAAGAGATCCAGAGGCTTGCTCGTGAGAATGCGAAAGATATCATTGCTTGTGGTTTTGATATTTCAAAAACCTTTATCTTCTCTGATTTTGATTACGTTGGAGGGTGAGTGTGATATGAGTGAACTTACTCTTAGAAGATAAGAGACATAAGACATCTAGCAGTCATTGAGTTGGAACTGGTGGATCTGTCTGAATATCTTATGCAAATTTCTAGGAGTGATTTATATACTCGCTGCCTGGAGTTGTTTCTAATTTTACGATTCTAGATTCATTGTTAGATAAAAAAAAGATCTAAGCATTTAGATTTCCTTAACTTACTTTATTTTGGGATTTGTTTCACAGTGCTTTCTATAAAAACATGGATAAAGTTGCCAAATGTGTTACATACAATAAGGTACTCACTTATAATCTAATTAGTTTTTTATATTATTTTATTTTAAAGCTTTCTTTATTAATATTATTATTATTTTGCTTATTTTGATATGTTGGTCTTGCTAGGTTGTAGGTATTTTTGGGTTTACCCGTGAAGATCATATTGGAAAAGTTAGTTTTCCACCGGTGCAGGTATGGGTCCCAACTTTGAAAATTTGATATCCTAGCATGGACAAGATTTAAGCCTTGTGTTTTGCTTGTGAATTCATATAAGTATGATGAGTTTCATTACAAGGCATGATGGCAGTATGAGGATCTTAAAGTTAACTTAGCATCTTTTGGTTTACTTGGAAGCATGTTTTGTTTGGATAGATTATGATTGGCTATGAATGTCTGCTTGAGTTGTGGAAGTGTGTGGATGTTGTGGTTGGGCAGTTTTGA

mRNA sequence

ATGCCGGCGACACGAGACATCCTACGACCTTCACTCGCGAACCTCTGGTTCGGCTTCACTCTCTGTTTCTCGCGCGAACTACAATACAAGACACTCGCGAACCTCCGACTTCACTCTCTGTTCTCTGTCTCTTCTCCCTCCGGTCATCTTCTCCCTCCGTCTGACAGTGTTCCTGCAAGTGCAGCAGCGGTTGGGTTGTCTTGTTTCTTTGGCAATCCAGCTAATCTTCCCAGATTCCTGGAAAATTGGGGAGAAATTTTCCCAGCAATTTTCTCTCAAGCCAATCAGAACACTGCACCTAGGCTTGCTCGTGAGAATGCGAAAGATATCATTGCTTGTGGTTTTGATATTTCAAAAACCTTTATCTTCTCTGATTTTGATTACGTTGGAGGTGCTTTCTATAAAAACATGGATAAAGTTGCCAAATGTGTTACATACAATAAGGTTGTAGGTATTTTTGGGTTTACCCGTGAAGATCATATTGGAAAAGTTAGTTTTCCACCGGTGCAGATTATGATTGGCTATGAATGTCTGCTTGAGTTGTGGAAGTGTGTGGATGTTGTGGTTGGGCAGTTTTGA

Coding sequence (CDS)

ATGCCGGCGACACGAGACATCCTACGACCTTCACTCGCGAACCTCTGGTTCGGCTTCACTCTCTGTTTCTCGCGCGAACTACAATACAAGACACTCGCGAACCTCCGACTTCACTCTCTGTTCTCTGTCTCTTCTCCCTCCGGTCATCTTCTCCCTCCGTCTGACAGTGTTCCTGCAAGTGCAGCAGCGGTTGGGTTGTCTTGTTTCTTTGGCAATCCAGCTAATCTTCCCAGATTCCTGGAAAATTGGGGAGAAATTTTCCCAGCAATTTTCTCTCAAGCCAATCAGAACACTGCACCTAGGCTTGCTCGTGAGAATGCGAAAGATATCATTGCTTGTGGTTTTGATATTTCAAAAACCTTTATCTTCTCTGATTTTGATTACGTTGGAGGTGCTTTCTATAAAAACATGGATAAAGTTGCCAAATGTGTTACATACAATAAGGTTGTAGGTATTTTTGGGTTTACCCGTGAAGATCATATTGGAAAAGTTAGTTTTCCACCGGTGCAGATTATGATTGGCTATGAATGTCTGCTTGAGTTGTGGAAGTGTGTGGATGTTGTGGTTGGGCAGTTTTGA

Protein sequence

MPATRDILRPSLANLWFGFTLCFSRELQYKTLANLRLHSLFSVSSPSGHLLPPSDSVPASAAAVGLSCFFGNPANLPRFLENWGEIFPAIFSQANQNTAPRLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNMDKVAKCVTYNKVVGIFGFTREDHIGKVSFPPVQIMIGYECLLELWKCVDVVVGQF

Homology

[Close]

BLAST of Lag0032171 vs. NCBI nr
Match: XP_033509906.1 (tryptophan--tRNA ligase, cytoplasmic-like isoform X2 [Nicotiana tomentosiformis])

HSP 1 Score: 144.4 bits (363), Expect = 9.9e-31
Identity = 67/71 (94.37%), Postives = 69/71 (97.18%), Query Frame = 0


Query: 101 RLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNMDKVAKCVTYNKVVGIFGFTREDH 160
           RLARENAKDIIACGFD+SKTFIFSDFDYVGGAFYKNM KVAKCVTYNKVVGIFGFT EDH
Sbjct: 146 RLARENAKDIIACGFDVSKTFIFSDFDYVGGAFYKNMVKVAKCVTYNKVVGIFGFTGEDH 205

Query: 161 IGKVSFPPVQI 172
           IGKVSFPPVQ+
Sbjct: 206 IGKVSFPPVQV 216

[Close]

BLAST of Lag0032171 vs. NCBI nr
Match: KDO79879.1 (hypothetical protein CISIN_1g015582mg [Citrus sinensis])

HSP 1 Score: 144.1 bits (362), Expect = 1.3e-30
Identity = 68/73 (93.15%), Postives = 70/73 (95.89%), Query Frame = 0


Query: 101 RLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNMDKVAKCVTYNKVVGIFGFTREDH 160
           RLARENAKDIIACGFD++KTFIFSDFDYVGGAFYKNM KVAKCVTYNKVVGIFGFT EDH
Sbjct: 143 RLARENAKDIIACGFDVTKTFIFSDFDYVGGAFYKNMVKVAKCVTYNKVVGIFGFTGEDH 202

Query: 161 IGKVSFPPVQIMI 174
           IGKVSFPPVQ MI
Sbjct: 203 IGKVSFPPVQEMI 215

[Close]

BLAST of Lag0032171 vs. NCBI nr
Match: XP_008452422.1 (PREDICTED: tryptophan--tRNA ligase, cytoplasmic [Cucumis melo])

HSP 1 Score: 143.7 bits (361), Expect = 1.7e-30
Identity = 68/70 (97.14%), Postives = 68/70 (97.14%), Query Frame = 0


Query: 101 RLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNMDKVAKCVTYNKVVGIFGFTREDH 160
           RLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNM KVAKCVTYNKVVGIFGFT EDH
Sbjct: 149 RLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNMVKVAKCVTYNKVVGIFGFTGEDH 208

Query: 161 IGKVSFPPVQ 171
           IGKVSFPPVQ
Sbjct: 209 IGKVSFPPVQ 218

[Close]

BLAST of Lag0032171 vs. NCBI nr
Match: PPD91328.1 (hypothetical protein GOBAR_DD11728 [Gossypium barbadense])

HSP 1 Score: 143.7 bits (361), Expect = 1.7e-30
Identity = 68/70 (97.14%), Postives = 68/70 (97.14%), Query Frame = 0


Query: 101 RLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNMDKVAKCVTYNKVVGIFGFTREDH 160
           RLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNM KVAKCVTYNKVVGIFGFT EDH
Sbjct: 144 RLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNMVKVAKCVTYNKVVGIFGFTGEDH 203

Query: 161 IGKVSFPPVQ 171
           IGKVSFPPVQ
Sbjct: 204 IGKVSFPPVQ 213

[Close]

BLAST of Lag0032171 vs. NCBI nr
Match: XP_023537341.1 (tryptophan--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] >KAG6600855.1 Tryptophan--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031490.1 Tryptophan--tRNA ligase, cytoplasmic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 143.7 bits (361), Expect = 1.7e-30
Identity = 68/70 (97.14%), Postives = 68/70 (97.14%), Query Frame = 0


Query: 101 RLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNMDKVAKCVTYNKVVGIFGFTREDH 160
           RLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNM KVAKCVTYNKVVGIFGFT EDH
Sbjct: 149 RLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNMVKVAKCVTYNKVVGIFGFTGEDH 208

Query: 161 IGKVSFPPVQ 171
           IGKVSFPPVQ
Sbjct: 209 IGKVSFPPVQ 218

[Close]

BLAST of Lag0032171 vs. ExPASy Swiss-Prot
Match: Q9SR15 (Tryptophan--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g04600 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 9.6e-29
Identity = 59/82 (71.95%), Postives = 68/82 (82.93%), Query Frame = 0


Query: 89  AIFSQANQNTAPRLARENAKDIIACGFDISKTFIFSDFDYVGGAFYKNMDKVAKCVTYNK 148
           +I+   +   + RLARENAKDIIACGFD++KTFIFSDFDYVGGAFYKNM KV KCVT NK
Sbjct: 134 SIWKNLSVEESQRLARENAKDIIACGFDVTKTFIFSDFDYVGGAFYKNMVKVGKCVTLNK 193

Query: 149 VVGIFGFTREDHIGKVSFPPVQ 171
            +GIFGF+ ED I K+SFPPVQ
Sbjct: 194 AMGIFGFSGEDPIAKLSFPPVQ 215

[Close]

BLAST of Lag0032171 vs. ExPASy Swiss-Prot
Match: P23381 (Tryptophan--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=WARS1 PE=1 SV=2)

HSP 1 Score: 99.8 bits (247), Expect = 3.7e-20
Identity = 48/70 (68.57%), Postives = 56/70 (80.00%), Query Frame = 0


Query: 103 ARENAKDIIACGFDISKTFIFSDFDYVG--GAFYKNMDKVAKCVTYNKVVGIFGFTREDH 162
           A ENAKDIIACGFDI+KTFIFSD DY+G    FYKN+ K+ K VT+N+V GIFGFT  D 
Sbjct: 215 AVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDC 274

Query: 163 IGKVSFPPVQ 171
           IGK+SFP +Q
Sbjct: 275 IGKISFPAIQ 284

[Close]

BLAST of Lag0032171 vs. ExPASy Swiss-Prot
Match: Q5R4J1 (Tryptophan--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=WARS1 PE=2 SV=2)

HSP 1 Score: 99.8 bits (247), Expect = 3.7e-20
Identity = 48/70 (68.57%), Postives = 56/70 (80.00%), Query Frame = 0


Query: 103 ARENAKDIIACGFDISKTFIFSDFDYVG--GAFYKNMDKVAKCVTYNKVVGIFGFTREDH 162
           A ENAKDIIACGFDI+KTFIFSD DY+G    FYKN+ K+ K VT+N+V GIFGFT  D 
Sbjct: 216 AVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDC 275

Query: 163 IGKVSFPPVQ 171
           IGK+SFP +Q
Sbjct: 276 IGKISFPAIQ 285

[Close]

BLAST of Lag0032171 vs. ExPASy Swiss-Prot
Match: P17248 (Tryptophan--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=WARS1 PE=1 SV=3)

HSP 1 Score: 99.0 bits (245), Expect = 6.3e-20
Identity = 48/70 (68.57%), Postives = 56/70 (80.00%), Query Frame = 0


Query: 103 ARENAKDIIACGFDISKTFIFSDFDYVG--GAFYKNMDKVAKCVTYNKVVGIFGFTREDH 162
           A ENAKDIIACGFDI+KTFIFSD DY+G    FYKN+ K+ K VT+N+V GIFGFT  D 
Sbjct: 220 AVENAKDIIACGFDINKTFIFSDLDYMGMSPGFYKNVVKIQKHVTFNQVKGIFGFTDSDC 279

Query: 163 IGKVSFPPVQ 171
           IGK+SFP +Q
Sbjct: 280 IGKISFPAIQ 289

[Close]

BLAST of Lag0032171 vs. ExPASy Swiss-Prot
Match: P23612 (Tryptophan--tRNA ligase, cytoplasmic OS=Oryctolagus cuniculus OX=9986 GN=WARS1 PE=2 SV=3)

HSP 1 Score: 98.2 bits (243), Expect = 1.1e-19
Identity = 46/68 (67.65%), Postives = 55/68 (80.88%), Query Frame = 0


Query: 105 ENAKDIIACGFDISKTFIFSDFDYVG--GAFYKNMDKVAKCVTYNKVVGIFGFTREDHIG 164
           ENAKDIIACGFD++KTFIFSD DY+G    FYKN+ K+ K VT+N+V GIFGFT  D IG
Sbjct: 221 ENAKDIIACGFDVNKTFIFSDLDYMGMSPGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIG 280

Query: 165 KVSFPPVQ 171
           K+SFP +Q
Sbjct: 281 KISFPAIQ 288

The following BLAST results are available for this feature:

BLAST of Lag0032171 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_033509906.1	9.9e-31	94.37	tryptophan--tRNA ligase, cytoplasmic-like isoform X2 [Nicotiana tomentosiformis]	[more]
KDO79879.1	1.3e-30	93.15	hypothetical protein CISIN_1g015582mg [Citrus sinensis]	[more]
XP_008452422.1	1.7e-30	97.14	PREDICTED: tryptophan--tRNA ligase, cytoplasmic [Cucumis melo]	[more]
PPD91328.1	1.7e-30	97.14	hypothetical protein GOBAR_DD11728 [Gossypium barbadense]	[more]
XP_023537341.1	1.7e-30	97.14	tryptophan--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] >KAG66008...	[more]

BLAST of Lag0032171 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SR15	9.6e-29	71.95	Tryptophan--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g0460...	[more]
P23381	3.7e-20	68.57	Tryptophan--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=WARS1 PE=1 SV=2	[more]
Q5R4J1	3.7e-20	68.57	Tryptophan--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=WARS1 PE=2 SV=2	[more]
P17248	6.3e-20	68.57	Tryptophan--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=WARS1 PE=1 SV=3	[more]
P23612	1.1e-19	67.65	Tryptophan--tRNA ligase, cytoplasmic OS=Oryctolagus cuniculus OX=9986 GN=WARS1 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 86..182 e-value: 1.9E-25 score: 91.5
None	No IPR available	PANTHER	PTHR10055	TRYPTOPHANYL-TRNA SYNTHETASE	coord: 96..173
None	No IPR available	PANTHER	PTHR10055:SF10	SUBFAMILY NOT NAMED	coord: 96..173
None	No IPR available	SUPERFAMILY	52374	Nucleotidylyl transferase	coord: 94..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0032171.1	Lag0032171.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006436	tryptophanyl-tRNA aminoacylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0004830	tryptophan-tRNA ligase activity