Lag0031962 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031962
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioncytochrome P450, family 76, subfamily C, polypeptide 2
Locationchr11: 21058298 .. 21059873 (-)
RNA-Seq ExpressionLag0031962
SyntenyLag0031962
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCCATTTATCCTCCTCCTCCTCCTCCTCCTCCTCCTCAAGCTCCTCAAATCTCGGAAACACTCCCCTCCACTTCCACCAGGCCCAACTCCGTGGCCCTTACTCGGCAACCTCCTCCAGATCGGCCCCAACCCCCTCCCCGCCATCACCCACTTCTCCACCCTCCACGGCCCTCTCATCTCCCTCCGCCTCGGCTCCCAGCTCGCCGTCGTCGCCTCCACCCCCTCCGCTGCCGCCGAGATCCTCCGCACCCACGACCGCACTCTCTCCGGGCGTTACATTTTCCAAACCGTCACCGACGCCCACAAACTCCGCCGCTTCTCCCTTCTACTCTCCCCGGAATGCGACGAGCACTGGAAGTTCCTCCGCACGACATGTCGCAGCAGCCTCTTCTCCAGCAAGGCCCTGGACTCGCAGGCCGCTCTGAGGACAAACAAGGCTCTGGCCTTGGTCCACTACTTGGCCTCCAAATCTGAAACAGGGGAGGCTGTGGCGATCAGAGACGTGGCCTTCACCACCGTGTTCAACACTATGACGAATGTTATCTTTTCCAAGGACCTCATGGAGTATGAGGGCGACGGGGCTTATGGATTCAAAGAGCATTATAGGAAGACGTTGGAGTTGGGTCTGGTTCCTAATTTGGCAGACTTTTATCCCATTTTGAGTGGGTTGGATGTTCAGGGTCTAAGGAAGAAATCGGCCGAGTGTAGCCGCCGCGTGGATTCTGTTTACGATACTTTTATTTCTGAGAGGAGGAGAGATCGTGATCGGAATGGTGGAGTAGCTACATGTCGGGATTTCTTGGATTCCATGCTTGATTTTGGTTTCTGTGATGATCAAATGAGGTACTTGATGGCTGTAAGTTCCCATTTTCCAAACTCTCTTTCTTTTATTTTCTTTTGTTTTATTTTCTTTCCTTTAATTTTCAAGTATTTATGGGTTCAATTTGCAGGAGATTCTTTCCGCCAGTACAGACACTACATCGACAACCGTGGAATGGACCATGGCAGAGTTGATGAAGAACAAACACATAATGCACAAAGTTCAAGAGGAGATCACAAGAGAAATAAAGGATCAAAATGCCGCCATTCATGAATCCATGATCCCACAACTTCATTATTTGCAAGCATGTGTGAAGGAAACTCTAAGATTACATCCACCCGCCCCTTTCCTTCTCCCCCACAGAGCTCTCCAAACATGTCAAGTAATGGGCTACACAATCCCTAAAGACACTCGCATTTTCGTCAACATTTGGGCCATCGGACGCCACCCGACAGCGTGGGAGAACCCCTCGACATTCAATCCGGATCGGTTTCTAGGATCGGATATCGAATTCAAAGGGTTCGACTTCCGGTATCTGCCATTCGGTTCGGGGAGAAGGATATGTCCTGGTCTTCCCATGGGTGTAGTGCAAGTCAACTTAATTTTAGCCACTTTGATTAGAAACTTTGATTGGTCTCTGCCTAATAATGAGGATCCTTCAAAGCTGGATCTAACTGGAAAAATGGGCATTGCATATTATAAAGAGAAGCCTCTGTCTTTGATTCCTAAAATCAGAGTTACTTCCTCCTAG

mRNA sequence

ATGGCCCCATTTATCCTCCTCCTCCTCCTCCTCCTCCTCCTCAAGCTCCTCAAATCTCGGAAACACTCCCCTCCACTTCCACCAGGCCCAACTCCGTGGCCCTTACTCGGCAACCTCCTCCAGATCGGCCCCAACCCCCTCCCCGCCATCACCCACTTCTCCACCCTCCACGGCCCTCTCATCTCCCTCCGCCTCGGCTCCCAGCTCGCCGTCGTCGCCTCCACCCCCTCCGCTGCCGCCGAGATCCTCCGCACCCACGACCGCACTCTCTCCGGGCGTTACATTTTCCAAACCGTCACCGACGCCCACAAACTCCGCCGCTTCTCCCTTCTACTCTCCCCGGAATGCGACGAGCACTGGAAGTTCCTCCGCACGACATGTCGCAGCAGCCTCTTCTCCAGCAAGGCCCTGGACTCGCAGGCCGCTCTGAGGACAAACAAGGCTCTGGCCTTGGTCCACTACTTGGCCTCCAAATCTGAAACAGGGGAGGCTGTGGCGATCAGAGACGTGGCCTTCACCACCGTGTTCAACACTATGACGAATGTTATCTTTTCCAAGGACCTCATGGAGTATGAGGGCGACGGGGCTTATGGATTCAAAGAGCATTATAGGAAGACGTTGGAGTTGGGTCTGGTTCCTAATTTGGCAGACTTTTATCCCATTTTGAGTGGGTTGGATGTTCAGGGTCTAAGGAAGAAATCGGCCGAGTGTAGCCGCCGCGTGGATTCTGTTTACGATACTTTTATTTCTGAGAGGAGGAGAGATCGTGATCGGAATGGTGGAGTAGCTACATGTCGGGATTTCTTGGATTCCATGCTTGATTTTGGTTTCTGTGATGATCAAATGAGGTACTTGATGGCTGAGATTCTTTCCGCCAGTACAGACACTACATCGACAACCGTGGAATGGACCATGGCAGAGTTGATGAAGAACAAACACATAATGCACAAAGTTCAAGAGGAGATCACAAGAGAAATAAAGGATCAAAATGCCGCCATTCATGAATCCATGATCCCACAACTTCATTATTTGCAAGCATGTGTGAAGGAAACTCTAAGATTACATCCACCCGCCCCTTTCCTTCTCCCCCACAGAGCTCTCCAAACATGTCAAGTAATGGGCTACACAATCCCTAAAGACACTCGCATTTTCGTCAACATTTGGGCCATCGGACGCCACCCGACAGCGTGGGAGAACCCCTCGACATTCAATCCGGATCGGTTTCTAGGATCGGATATCGAATTCAAAGGGTTCGACTTCCGGTATCTGCCATTCGGTTCGGGGAGAAGGATATGTCCTGGTCTTCCCATGGGTGTAGTGCAAGTCAACTTAATTTTAGCCACTTTGATTAGAAACTTTGATTGGTCTCTGCCTAATAATGAGGATCCTTCAAAGCTGGATCTAACTGGAAAAATGGGCATTGCATATTATAAAGAGAAGCCTCTGTCTTTGATTCCTAAAATCAGAGTTACTTCCTCCTAG

Coding sequence (CDS)

ATGGCCCCATTTATCCTCCTCCTCCTCCTCCTCCTCCTCCTCAAGCTCCTCAAATCTCGGAAACACTCCCCTCCACTTCCACCAGGCCCAACTCCGTGGCCCTTACTCGGCAACCTCCTCCAGATCGGCCCCAACCCCCTCCCCGCCATCACCCACTTCTCCACCCTCCACGGCCCTCTCATCTCCCTCCGCCTCGGCTCCCAGCTCGCCGTCGTCGCCTCCACCCCCTCCGCTGCCGCCGAGATCCTCCGCACCCACGACCGCACTCTCTCCGGGCGTTACATTTTCCAAACCGTCACCGACGCCCACAAACTCCGCCGCTTCTCCCTTCTACTCTCCCCGGAATGCGACGAGCACTGGAAGTTCCTCCGCACGACATGTCGCAGCAGCCTCTTCTCCAGCAAGGCCCTGGACTCGCAGGCCGCTCTGAGGACAAACAAGGCTCTGGCCTTGGTCCACTACTTGGCCTCCAAATCTGAAACAGGGGAGGCTGTGGCGATCAGAGACGTGGCCTTCACCACCGTGTTCAACACTATGACGAATGTTATCTTTTCCAAGGACCTCATGGAGTATGAGGGCGACGGGGCTTATGGATTCAAAGAGCATTATAGGAAGACGTTGGAGTTGGGTCTGGTTCCTAATTTGGCAGACTTTTATCCCATTTTGAGTGGGTTGGATGTTCAGGGTCTAAGGAAGAAATCGGCCGAGTGTAGCCGCCGCGTGGATTCTGTTTACGATACTTTTATTTCTGAGAGGAGGAGAGATCGTGATCGGAATGGTGGAGTAGCTACATGTCGGGATTTCTTGGATTCCATGCTTGATTTTGGTTTCTGTGATGATCAAATGAGGTACTTGATGGCTGAGATTCTTTCCGCCAGTACAGACACTACATCGACAACCGTGGAATGGACCATGGCAGAGTTGATGAAGAACAAACACATAATGCACAAAGTTCAAGAGGAGATCACAAGAGAAATAAAGGATCAAAATGCCGCCATTCATGAATCCATGATCCCACAACTTCATTATTTGCAAGCATGTGTGAAGGAAACTCTAAGATTACATCCACCCGCCCCTTTCCTTCTCCCCCACAGAGCTCTCCAAACATGTCAAGTAATGGGCTACACAATCCCTAAAGACACTCGCATTTTCGTCAACATTTGGGCCATCGGACGCCACCCGACAGCGTGGGAGAACCCCTCGACATTCAATCCGGATCGGTTTCTAGGATCGGATATCGAATTCAAAGGGTTCGACTTCCGGTATCTGCCATTCGGTTCGGGGAGAAGGATATGTCCTGGTCTTCCCATGGGTGTAGTGCAAGTCAACTTAATTTTAGCCACTTTGATTAGAAACTTTGATTGGTCTCTGCCTAATAATGAGGATCCTTCAAAGCTGGATCTAACTGGAAAAATGGGCATTGCATATTATAAAGAGAAGCCTCTGTCTTTGATTCCTAAAATCAGAGTTACTTCCTCCTAG

Protein sequence

MAPFILLLLLLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLISLRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWKFLRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRVDSVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTVEWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFLLPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFRYLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKPLSLIPKIRVTSS
Homology
[Close]
BLAST of Lag0031962 vs. NCBI nr
Match: XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])

HSP 1 Score: 539.7 bits (1389), Expect = 2.7e-149
Identity = 271/488 (55.53%), Postives = 353/488 (72.34%), Query Frame = 0 

Query: 4   FILLLLLLL--LLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLI 63
           F+LLLL L+  +L+ + S    PPLPPGP PWP+LGNLLQIG N   ++T FS  +GPLI
Sbjct: 8   FLLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 67

Query: 64  SLRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWK 123
           SL+LG+Q  VVAS+P+AAA IL+T DR LS RYIFQ   D       SLL SPEC + WK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 124 FLRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTN 183
            LR+ CR+ LF+++A++SQA+LR  K   +V +L SK   G  V I+++ F TVFNT++N
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQ--GSVVEIKELVFITVFNTLSN 187

Query: 184 VIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRV 243
            IFS+DL+ + GDG  G K  ++  ++LGL+PNLADFYP+L   D+QGLRKK+A    +V
Sbjct: 188 FIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQV 247

Query: 244 DSVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTV 303
           DS ++  I ERR    R+  V+   DFLD +++ GF D+Q+ YL+ E+LSA TDTT+TTV
Sbjct: 248 DSSWEILIKERRTIHSRD--VSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTV 307

Query: 304 EWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFL 363
           EW MAEL+KN+ IM++V+EE  + I     AI ES + +LHYL  CVKETLRLHPPAPFL
Sbjct: 308 EWAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFL 367

Query: 364 LPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFR 423
           LP  A + C++MGY++PKDT IFVN+W IGR P+ WE+P +F P+RF G D + KG D+R
Sbjct: 368 LPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYR 427

Query: 424 YLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKP 483
           +LPFG GRRICPGLPM  VQV LILATLI NF+W LPN+ED S LDL G++G+   KE+P
Sbjct: 428 FLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEP 487

Query: 484 LSLIPKIR 490
           L L+ K R
Sbjct: 488 LKLLSKKR 489

[Close]
BLAST of Lag0031962 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 538.1 bits (1385), Expect = 7.9e-149
Identity = 267/485 (55.05%), Postives = 350/485 (72.16%), Query Frame = 0 

Query: 5   ILLLLLLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLISLR 64
           +LL L+ ++L+ + S    PPLPPGP PWP+LGNLLQIG N   ++T FS ++GPLISL+
Sbjct: 11  LLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLISLK 70

Query: 65  LGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWKFLR 124
           LG+Q  VVAS+P+AAA IL+T DR LS RYIFQ   D       SLL SPEC + WK LR
Sbjct: 71  LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWKNLR 130

Query: 125 TTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTNVIF 184
           + CR+ LF+++A++SQA+LR  K   +V +L SK   G  V I+++ F TVFNT++N IF
Sbjct: 131 SICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQ--GSVVEIKELVFITVFNTLSNFIF 190

Query: 185 SKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRVDSV 244
           S+DL+ + GDG  G K  ++  ++LGL+PNLADFYP+L   D+QGLRKK+A    +VDS 
Sbjct: 191 SRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDSS 250

Query: 245 YDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTVEWT 304
           ++  I ERR    +  GV+   DFLD ++  GF D+Q+ YL+ E+LSA TDTT+TTVEW 
Sbjct: 251 WEILIKERRTIHSQ--GVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWA 310

Query: 305 MAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFLLPH 364
           MAEL+KN+ IM++V+EE  + I     AI ES + +LHYL  CVKETLRLHPPAPFLLP 
Sbjct: 311 MAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPR 370

Query: 365 RALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFRYLP 424
            A + C++MGY++PK T IFVN+W IGR P+ WE+P +F P+RF G D + KG D+R+LP
Sbjct: 371 YAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLP 430

Query: 425 FGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKPLSL 484
           FG GRRICPGLPM  VQV LILATLI NF+W LPN+ED S LDL G++G+   KE+PL L
Sbjct: 431 FGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKL 489

Query: 485 IPKIR 490
           + K R
Sbjct: 491 LSKNR 489

[Close]
BLAST of Lag0031962 vs. NCBI nr
Match: XP_023551257.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535.4 bits (1378), Expect = 5.1e-148
Identity = 267/487 (54.83%), Postives = 350/487 (71.87%), Query Frame = 0 

Query: 4   FILLL-LLLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLIS 63
           F+LLL L+  +LK + S    PPLPPGP PWP+LGNLLQIG N   ++T FS ++GPLIS
Sbjct: 8   FLLLLPLIFTVLKHISSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHIYGPLIS 67

Query: 64  LRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWKF 123
           L+LG+Q  VVAS+P+AAA IL+T DR LS RYI Q   D       SLL SPEC + WK 
Sbjct: 68  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYILQMTPDRALHDNCSLLFSPECGDRWKN 127

Query: 124 LRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTNV 183
           LR+ CR+ LF+++A++SQA+LR  K   +V +L SK   G  V I++  F TVFNT++N+
Sbjct: 128 LRSICRAKLFTARAIESQASLRRKKMKDMVEFLQSKQ--GSVVEIKEFVFITVFNTLSNL 187

Query: 184 IFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRVD 243
           IFS+DL+++ GDG  G K  ++  ++LGL+PNLADFYP+L   D+QGLRKK+A    ++D
Sbjct: 188 IFSRDLVDFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQID 247

Query: 244 SVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTVE 303
           S  +  I ERR    R  GV+   DFLD +++ GF ++Q+ YL+ E+L A TDTT+TTVE
Sbjct: 248 SSSEILIKERRTIHSR--GVSAASDFLDILIESGFTNEQISYLILEVLLAGTDTTTTTVE 307

Query: 304 WTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFLL 363
           W MAELMKN+ IM++V+EE  + +     AI ES + +LHYL  CVKET+RLHPPAPFLL
Sbjct: 308 WAMAELMKNRDIMNRVREETKKAL--NGGAIDESKLSELHYLWKCVKETVRLHPPAPFLL 367

Query: 364 PHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFRY 423
           P  A + C++MGY++PK T IFVN+W IGR P+ WE+P +F P+RF  SD + KG D+R+
Sbjct: 368 PRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDSSDADLKGHDYRF 427

Query: 424 LPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKPL 483
           LPFG GRR CPGLPM  VQV LILATLI NF+W LPN+ED S LDL G++GI   KE+PL
Sbjct: 428 LPFGGGRRTCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGITLQKEEPL 487

Query: 484 SLIPKIR 490
            L+PK R
Sbjct: 488 KLVPKKR 488

[Close]
BLAST of Lag0031962 vs. NCBI nr
Match: KAG7017133.1 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 533.5 bits (1373), Expect = 1.9e-147
Identity = 268/489 (54.81%), Postives = 352/489 (71.98%), Query Frame = 0 

Query: 4   FILLLLLLLLLKLLK---SRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPL 63
           F+LLLLL L++ +LK   S    PPLPPGP PWP+LGNLLQIG N   ++T FS ++GPL
Sbjct: 8   FLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPL 67

Query: 64  ISLRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHW 123
           ISL+LG+Q  VVAS+P+AAA IL+T DR LS RYIFQ + D       SL+ SPEC + W
Sbjct: 68  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRW 127

Query: 124 KFLRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMT 183
           K LR+ CR+ LF+++A++SQA+LR  K   +V +L SK   G  V I++  F TVFNT++
Sbjct: 128 KNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQ--GSVVEIKEFVFITVFNTLS 187

Query: 184 NVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRR 243
           N IFS+DL+ + GDG  G K H++  ++L L+PNLADFYP+L   D+QGLRKK+A    +
Sbjct: 188 NFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQ 247

Query: 244 VDSVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTT 303
           +DS ++  I ERR    R  GV+   DFLD ++  G+ ++Q+ YL+ E+LSA TDT  TT
Sbjct: 248 IDSSWEILIKERRTIHSR--GVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTDT--TT 307

Query: 304 VEWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPF 363
           VEW MAEL+KN+ IM++V+EE  + I     AI ES + +LHYL  CVKETLRLHPPAPF
Sbjct: 308 VEWAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPF 367

Query: 364 LLPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDF 423
           LLP  A + C++MGY++PK T IFVN+W IGR P+ WE+P +F P+RF G D + KG D+
Sbjct: 368 LLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDY 427

Query: 424 RYLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEK 483
           R+LPFG GRR+CPGLPM  VQV LILATLI NF+W LPN+E+ S LDL G++GI   KE+
Sbjct: 428 RFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEE 487

Query: 484 PLSLIPKIR 490
           PL L+PK R
Sbjct: 488 PLKLLPKKR 488

[Close]
BLAST of Lag0031962 vs. NCBI nr
Match: XP_031741123.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 531.9 bits (1369), Expect = 5.7e-147
Identity = 268/493 (54.36%), Postives = 357/493 (72.41%), Query Frame = 0 

Query: 1   MAPFILLLL--LLLLLKLLKSRK-HSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLH 60
           + P +LLL+  + ++LK +KS      PLPPGP PWP+LGNL QIG N   +IT F+ ++
Sbjct: 9   LLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIY 68

Query: 61  GPLISLRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECD 120
           GPLISL+LG+Q  VVAS+P+ A  +L+THDR LS RYI Q   D     + SL+ SPEC 
Sbjct: 69  GPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECG 128

Query: 121 EHWKFLRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFN 180
           +HWK LR+ C+++LF++KA++SQA LR  K   LV +L SK   G  V I+D  FT+VFN
Sbjct: 129 DHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQ--GSVVKIKDFVFTSVFN 188

Query: 181 TMTNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAEC 240
           T++N+IFS DL+++ GDG  G K  ++K ++LGL PNLADFYP+L   D+QGLRKK+A  
Sbjct: 189 TLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIY 248

Query: 241 SRRVDSVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTT 300
              VDS++   I ERR    + G  ++  DFLD ++  GF D Q+ YL+ E+LSA TDTT
Sbjct: 249 MNEVDSLWGILIKERREIHCQQGSASS--DFLDVLIQSGFSDQQINYLINELLSAGTDTT 308

Query: 301 STTVEWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPP 360
           +TTVEW M+E++K+K I++KV++EI +  K+  + I ES I +L YL +CVKETLRLHPP
Sbjct: 309 TTTVEWAMSEILKDKGILNKVRQEIKKVTKE--SPIDESNISKLQYLWSCVKETLRLHPP 368

Query: 361 APFLLPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRF---LGSDIE 420
           APFLLP  A   C+VMGY+IPKD+ IFVN+W IGR P+ WE+  TFNP+RF    G++++
Sbjct: 369 APFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVD 428

Query: 421 FKGFDFRYLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGI 480
           FKG+D+RYLPFG GRRICPGLPM +VQV LILATL+ NF+WSLPN ED SKLDL G++G+
Sbjct: 429 FKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGV 488

Query: 481 AYYKEKPLSLIPK 488
              K + L LIPK
Sbjct: 489 TLQKYEALELIPK 495

[Close]
BLAST of Lag0031962 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 5.6e-113
Identity = 213/487 (43.74%), Postives = 306/487 (62.83%), Query Frame = 0 

Query: 6   LLLLLLLLLKLLKSRKHSPP-LPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLISLR 65
           L+ +++     L  R  SP  LPPGP PWP++GNLLQ+G  P       +  +G L SL+
Sbjct: 2   LVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLK 61

Query: 66  LGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWKFLR 125
           LGS+  VVASTP AA+EIL+THDR LSGRY+FQ+      +   + ++  EC+E WK LR
Sbjct: 62  LGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVE--NSIVWSECNETWKKLR 121

Query: 126 TTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTNVIF 185
             CR+ LF+ K ++SQA +R +KA+ +V YL  K   G  V I +V F T+ N   N+IF
Sbjct: 122 KVCRTELFTQKMIESQAEVRESKAMEMVEYL--KKNVGNEVKIAEVVFGTLVNIFGNLIF 181

Query: 186 SKDLMEY--EGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRVD 245
           S+++ +   E  G+   KEH  + LELG   N AD++P L   D+ G  K  A+C + + 
Sbjct: 182 SQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIY 241

Query: 246 SVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTVE 305
           SV+   + ER+  +  N   +   DF++ +LD G  D Q+  L+ EI  A T+T+++T+E
Sbjct: 242 SVWGAMLKERKIAKQHNN--SKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIE 301

Query: 306 WTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFLL 365
           W ++EL KN  +   ++ E+   +  +   + ES IP + YLQA VKETLRLHP  P LL
Sbjct: 302 WALSELTKNPQVTANMRLELLSVVGKR--PVKESDIPNMPYLQAFVKETLRLHPATPLLL 361

Query: 366 PHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFRY 425
           P RAL+TC+V+ YTIPK+ +I VN W IGR P  W +P  F+P+RFL S I+FKG DF  
Sbjct: 362 PRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFEL 421

Query: 426 LPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKPL 485
           +PFG+GRRICPG+P+    ++LI+++L++NFDW  P   DPS+L +  K G+   KE PL
Sbjct: 422 IPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPL 480

Query: 486 SLIPKIR 490
            ++PK R
Sbjct: 482 YIVPKTR 480

[Close]
BLAST of Lag0031962 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.8e-112
Identity = 214/491 (43.58%), Postives = 309/491 (62.93%), Query Frame = 0 

Query: 4   FILLLLLLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLISL 63
           F  LL L  +L+  + +     LPPGP P P++GNLLQ+G  P       +  +G L SL
Sbjct: 11  FSFLLFLFFILRDSRPKN----LPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFSL 70

Query: 64  RLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWKFL 123
           +LGSQ  VVAS+P+AAAEIL+THD+ LSGRY+FQ+      +   + ++  EC+++WK L
Sbjct: 71  KLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVE--NSIVWSECNDNWKLL 130

Query: 124 RTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTNVI 183
           R  CR+ LF+ K ++SQ+ +R  KA  +V +L  K   GE V I +V F T+ N   N+I
Sbjct: 131 RKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKE--GEVVKIVEVVFGTLVNIFGNLI 190

Query: 184 FSKDLMEYEG--DGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRV 243
           FSKD+ + E    G+   KEH  K L++G   N AD++PI+  LD+ G R+  AE  +++
Sbjct: 191 FSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQI 250

Query: 244 DSVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTV 303
             V+   + ERR  +    G  +  DF+D +L+ G  D ++  L+ E+  A T+T+++T+
Sbjct: 251 YDVWGVMLKERRGTK----GSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTI 310

Query: 304 EWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFL 363
           EW + EL K   ++ K++ E+   + D    + ES +P L YLQA VKETLRLHPP P L
Sbjct: 311 EWAITELTKKPLVVSKIRLELVNVVGDN--TVKESDLPHLPYLQAFVKETLRLHPPTPLL 370

Query: 364 LPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFR 423
           LP RAL+TC VM YTIPK+ +I VN WAIGR P  W++P  F P+RFL SD+++KG DF 
Sbjct: 371 LPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFE 430

Query: 424 YLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKP 483
            +PFG GRRICPGLP+     NLI+ATL++NF+WSLP     S+L +  K G+   K+ P
Sbjct: 431 LIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPP 487

Query: 484 LSLIPKIRVTS 493
           L ++ K R ++
Sbjct: 491 LLIVLKARASN 487

[Close]
BLAST of Lag0031962 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 7.5e-110
Identity = 208/488 (42.62%), Postives = 303/488 (62.09%), Query Frame = 0 

Query: 5   ILLLLLLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLISLR 64
           ++ +++  +L LL        LPPGP PWP++GNLLQ+G  P       +  +G + +L+
Sbjct: 7   LIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLK 66

Query: 65  LGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWKFLR 124
           +G++  VVAST SAA+EIL+THDR LS RY+FQ+      +   + ++  +C E WK LR
Sbjct: 67  MGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVE--NSIVWSDCTETWKNLR 126

Query: 125 TTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTNVIF 184
             CR+ LF+ K ++SQA +R  K   +V YL  K   GE V I +V F T+ N   N+IF
Sbjct: 127 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQ--GEEVKIVEVIFGTLVNIFGNLIF 186

Query: 185 SKDLMEY--EGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRVD 244
           S+++ E      G+  FKE+  + LELG   N AD++P+L   D+ G RK+ AEC + + 
Sbjct: 187 SQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIY 246

Query: 245 SVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTVE 304
           +++   + ER+  + +  G  +  DF+D  LD G  D Q+  L+ E+  A T+T+++T+E
Sbjct: 247 AIWGAMLQERKLAK-KVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIE 306

Query: 305 WTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFLL 364
           W M EL KN  I  K++ EI   + ++  ++ ES  P L YL+A VKETLRLHPP P LL
Sbjct: 307 WAMTELTKNPKITAKIRSEIQTVVGER--SVKESDFPNLPYLEATVKETLRLHPPTPLLL 366

Query: 365 PHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFRY 424
           P RAL+TC ++ YTIPKD +I VN W IGR P  W +P TF+P+RFL S ++F+G DF  
Sbjct: 367 PRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSL 426

Query: 425 LPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKPL 484
           +PFG+GRRICPGLP+    + L++AT ++N DW LPN      L +  K G+   KE PL
Sbjct: 427 IPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPL 486

Query: 485 SLIPKIRV 491
            ++PK RV
Sbjct: 487 FIVPKSRV 487

[Close]
BLAST of Lag0031962 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 2.9e-109
Identity = 207/488 (42.42%), Postives = 303/488 (62.09%), Query Frame = 0 

Query: 5   ILLLLLLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLISLR 64
           ++ +++  +L LL        LPPGP PWP++GNLLQ+G  P       +  +G + +L+
Sbjct: 8   LIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLK 67

Query: 65  LGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWKFLR 124
           +G++  VVAST SAA+EIL+THDR LS RY+FQ+      +   + ++  +C E WK LR
Sbjct: 68  MGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVE--NSIVWSDCTETWKNLR 127

Query: 125 TTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTNVIF 184
             CR+ LF+ K ++SQA +R  K   +V YL  K   GE V I +V F T+ N   N+IF
Sbjct: 128 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQ--GEEVKIVEVIFGTLVNIFGNLIF 187

Query: 185 SKDLME--YEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRVD 244
           S+++ E      G+  FKE+  + LELG   N AD++P+L   D+ G RK+ AEC + + 
Sbjct: 188 SQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIY 247

Query: 245 SVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTVE 304
           +++   + ER+  + +  G  +  DF+D  LD G  D Q+  L+ E+  A T+T+++T+E
Sbjct: 248 AIWGAMLQERKLAK-KVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIE 307

Query: 305 WTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFLL 364
           W M EL KN  I  K++ E+   + ++  ++ ES  P L YL+A VKETLRLHPP P LL
Sbjct: 308 WAMTELTKNPKITAKLRSELQTVVGER--SVKESDFPNLPYLEATVKETLRLHPPTPLLL 367

Query: 365 PHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFRY 424
           P RAL+TC ++ YTIPKD +I VN W IGR P  W +P TF+P+RFL S ++F+G DF  
Sbjct: 368 PRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSL 427

Query: 425 LPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKPL 484
           +PFG+GRRICPGLP+    + L++AT ++N DW LPN      L +  K G+   KE PL
Sbjct: 428 IPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPL 487

Query: 485 SLIPKIRV 491
            ++PK RV
Sbjct: 488 FIVPKSRV 488

[Close]
BLAST of Lag0031962 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.2e-100
Identity = 203/494 (41.09%), Postives = 306/494 (61.94%), Query Frame = 0 

Query: 1   MAPFILLLLLLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPN---PLP-AITHFSTL 60
           +A F L+ ++L+ + LLK +  +  LPPGP PWPL+GNL  +  N   PL   + + +  
Sbjct: 5   IALFSLIPVILVFILLLKPKYKN--LPPGPHPWPLIGNLPILFTNTEVPLHITLANMART 64

Query: 61  HGPLISLRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPEC 120
           HGP++ L LG+Q  V+AST  AA EIL+THDR  S R+I  +    H + ++SL+ S +C
Sbjct: 65  HGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHI-KYSLVWS-DC 124

Query: 121 DEHWKFLRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVF 180
            ++WK LR   R+ +FS K L +Q+ +R  K   L+ +L SK   G+ V I    F T+ 
Sbjct: 125 TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKE--GQVVKISQFVFGTLL 184

Query: 181 NTMTNVIFSKDLMEY-EGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSA 240
           N + NV+FSKD+  Y +     G +   R+ L +G  PN+A+FYP L  LD+QGL+KK  
Sbjct: 185 NILGNVVFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCD 244

Query: 241 ECSRRVDSVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTD 300
           E   RV  +++  + ER+ +R+        +D LD +L   F D Q+  L  E     ++
Sbjct: 245 ERFIRVMKMWEGTVKERKANRNEES-----KDMLDVLLANDFNDAQINALFLETFGPGSE 304

Query: 301 TTSTTVEWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLH 360
           T+S T+EW +AEL+K+   M KV++E+   +    + I ES +PQL YLQAC+KE +RLH
Sbjct: 305 TSSATIEWVIAELIKSPKEMAKVRKELNEVV--GTSTIKESDLPQLPYLQACIKEAMRLH 364

Query: 361 PPAPFLLPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEF 420
           P APFLLP RA +TC+VMGYTIPK++++ VN +AIGR P +W++PSTF P+RFL SD++F
Sbjct: 365 PAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDF 424

Query: 421 KGFDFRYLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIA 480
            G  ++++PFGSGRR C G+P+    + LI+ +L+ N+D+ LP    P  L +   + + 
Sbjct: 425 HGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLT 484

Query: 481 YYKEKPLSLIPKIR 490
              +  L ++PK R
Sbjct: 485 LAIDPSLCVVPKAR 485

[Close]
BLAST of Lag0031962 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 1.3e-149
Identity = 271/488 (55.53%), Postives = 353/488 (72.34%), Query Frame = 0 

Query: 4   FILLLLLLL--LLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLI 63
           F+LLLL L+  +L+ + S    PPLPPGP PWP+LGNLLQIG N   ++T FS  +GPLI
Sbjct: 8   FLLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 67

Query: 64  SLRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWK 123
           SL+LG+Q  VVAS+P+AAA IL+T DR LS RYIFQ   D       SLL SPEC + WK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 124 FLRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTN 183
            LR+ CR+ LF+++A++SQA+LR  K   +V +L SK   G  V I+++ F TVFNT++N
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQ--GSVVEIKELVFITVFNTLSN 187

Query: 184 VIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRV 243
            IFS+DL+ + GDG  G K  ++  ++LGL+PNLADFYP+L   D+QGLRKK+A    +V
Sbjct: 188 FIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQV 247

Query: 244 DSVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTV 303
           DS ++  I ERR    R+  V+   DFLD +++ GF D+Q+ YL+ E+LSA TDTT+TTV
Sbjct: 248 DSSWEILIKERRTIHSRD--VSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTV 307

Query: 304 EWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFL 363
           EW MAEL+KN+ IM++V+EE  + I     AI ES + +LHYL  CVKETLRLHPPAPFL
Sbjct: 308 EWAMAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFL 367

Query: 364 LPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFR 423
           LP  A + C++MGY++PKDT IFVN+W IGR P+ WE+P +F P+RF G D + KG D+R
Sbjct: 368 LPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYR 427

Query: 424 YLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKP 483
           +LPFG GRRICPGLPM  VQV LILATLI NF+W LPN+ED S LDL G++G+   KE+P
Sbjct: 428 FLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEP 487

Query: 484 LSLIPKIR 490
           L L+ K R
Sbjct: 488 LKLLSKKR 489

[Close]
BLAST of Lag0031962 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 3.8e-149
Identity = 267/485 (55.05%), Postives = 350/485 (72.16%), Query Frame = 0 

Query: 5   ILLLLLLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLISLR 64
           +LL L+ ++L+ + S    PPLPPGP PWP+LGNLLQIG N   ++T FS ++GPLISL+
Sbjct: 11  LLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLISLK 70

Query: 65  LGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWKFLR 124
           LG+Q  VVAS+P+AAA IL+T DR LS RYIFQ   D       SLL SPEC + WK LR
Sbjct: 71  LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWKNLR 130

Query: 125 TTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTNVIF 184
           + CR+ LF+++A++SQA+LR  K   +V +L SK   G  V I+++ F TVFNT++N IF
Sbjct: 131 SICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQ--GSVVEIKELVFITVFNTLSNFIF 190

Query: 185 SKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRVDSV 244
           S+DL+ + GDG  G K  ++  ++LGL+PNLADFYP+L   D+QGLRKK+A    +VDS 
Sbjct: 191 SRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDSS 250

Query: 245 YDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTVEWT 304
           ++  I ERR    +  GV+   DFLD ++  GF D+Q+ YL+ E+LSA TDTT+TTVEW 
Sbjct: 251 WEILIKERRTIHSQ--GVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWA 310

Query: 305 MAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFLLPH 364
           MAEL+KN+ IM++V+EE  + I     AI ES + +LHYL  CVKETLRLHPPAPFLLP 
Sbjct: 311 MAELLKNRDIMNRVREETKKAI--NGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPR 370

Query: 365 RALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFRYLP 424
            A + C++MGY++PK T IFVN+W IGR P+ WE+P +F P+RF G D + KG D+R+LP
Sbjct: 371 YAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLP 430

Query: 425 FGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKPLSL 484
           FG GRRICPGLPM  VQV LILATLI NF+W LPN+ED S LDL G++G+   KE+PL L
Sbjct: 431 FGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKL 489

Query: 485 IPKIR 490
           + K R
Sbjct: 491 LSKNR 489

[Close]
BLAST of Lag0031962 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 6.1e-147
Identity = 264/488 (54.10%), Postives = 348/488 (71.31%), Query Frame = 0 

Query: 4   FILLL-LLLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLIS 63
           F+LLL L+  +LK + S    PP PPGP PWP+LGNLLQIG N   ++T FS ++GPLIS
Sbjct: 8   FLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLIS 67

Query: 64  LRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWKF 123
           L+LG+Q  VVAS+P+AAA IL+T DR LS RYIFQ + D       SL+ SPEC + WK 
Sbjct: 68  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKN 127

Query: 124 LRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTNV 183
           LR+ CR+ LF+++A++SQA+LR  K   +V +L SK   G  V I++  F TVFNT++N 
Sbjct: 128 LRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQ--GSVVEIKEFVFITVFNTLSNF 187

Query: 184 IFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRVD 243
           IFS+DL+ + GDG  G + H++  ++  L+PNLADFYP+L   D+QGLRKK+A    ++D
Sbjct: 188 IFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQID 247

Query: 244 SVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTSTTVE 303
           S ++  I ERR    R  GV    DFLD ++  GF D+Q+ YL+ E+LSA TDTT+TTVE
Sbjct: 248 SSWEILIKERRTIHSR--GVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVE 307

Query: 304 WTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFLL 363
           W MAEL+KN+ IM++V+EE  + I     AI ES + +LHYL  CVKETLRLHPPAPFLL
Sbjct: 308 WAMAELLKNRDIMNRVREETKKAINGD--AIDESKLSELHYLWKCVKETLRLHPPAPFLL 367

Query: 364 PHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFRY 423
           P  A + C++MGY++PK T I VN+W IGR P+ WE+P +F P+RF   D + KG D+R+
Sbjct: 368 PRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYRF 427

Query: 424 LPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKPL 483
           LPFG GRR+CPGLPM  VQV LILATLI NF+W LPN+ED S LDL G++G+   KE+PL
Sbjct: 428 LPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPL 487

Query: 484 SLIPKIRV 491
            L+PK R+
Sbjct: 488 KLLPKRRI 489

[Close]
BLAST of Lag0031962 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 1.2e-145
Identity = 267/495 (53.94%), Postives = 355/495 (71.72%), Query Frame = 0 

Query: 1   MAPFILLLL-LLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGP 60
           + P +LLLL + ++LK +KS     PLPPGP PWP+LGNLLQIG N   +IT F+ ++GP
Sbjct: 9   LLPSLLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGP 68

Query: 61  LISLRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEH 120
           LISL+LG+Q  +VAS+P+AA  +L+T DR LS RYIFQ   D     + SL+ SPEC + 
Sbjct: 69  LISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQ 128

Query: 121 WKFLRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTM 180
           WK LR+ C+ +LF++KA++SQA LR  K   LV +L SK   G  V ++D  FT+VFNT+
Sbjct: 129 WKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQ--GSVVEVKDFVFTSVFNTL 188

Query: 181 TNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSR 240
           +N+IFS DL+++ GDG  G K  ++K ++LGL PNLADFYP+L   D+QGL+ K+     
Sbjct: 189 SNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKN 248

Query: 241 RVDSVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTST 300
            VDS++   I ERR    + G  +   DFLD ++  GF D Q+  L+ E+LSA TDTT+T
Sbjct: 249 EVDSLWGILIKERREIHCQQGSAS--NDFLDVLIQSGFDDQQINALIIELLSAGTDTTTT 308

Query: 301 TVEWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAP 360
           TVEW M+E++K+K I++KV+EE+ +++  +N  I ES I +L YL  CVKETLRLHPPAP
Sbjct: 309 TVEWAMSEILKDKDILNKVREEM-KKVTKENRPIDESNISELQYLWRCVKETLRLHPPAP 368

Query: 361 FLLPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRF---LGSD-IEF 420
           FLLP  A   C+VMGY+IPKD+ +FVN+W IGR P+ WE+  TFNP RF    G+D ++F
Sbjct: 369 FLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDF 428

Query: 421 KGFDFRYLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIA 480
           KG+D+RYLPFG GRRICPGLPM +VQV LILATLI NF+WSLPN+ED SKLDL G++G+ 
Sbjct: 429 KGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVT 488

Query: 481 YYKEKPLSLIPKIRV 491
             K + L LIPK RV
Sbjct: 489 LQKYETLKLIPKRRV 498

[Close]
BLAST of Lag0031962 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 1.5e-145
Identity = 267/495 (53.94%), Postives = 355/495 (71.72%), Query Frame = 0 

Query: 1   MAPFILLLL-LLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGP 60
           + P +LLLL + ++LK +KS     PLPPGP PWP+LGNLLQIG N   +IT F+ ++GP
Sbjct: 9   LLPSLLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGP 68

Query: 61  LISLRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEH 120
           LISL+LG+Q  +VAS+P+AA  +L+T DR LS RYIFQ   D     + SL+ SPEC + 
Sbjct: 69  LISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQ 128

Query: 121 WKFLRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTM 180
           WK LR+ C+ +LF++KA++SQA LR  K   LV +L SK   G  V ++D  FT+VFNT+
Sbjct: 129 WKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQ--GSVVEVKDFVFTSVFNTL 188

Query: 181 TNVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSR 240
           +N+IFS DL+++ GDG  G K  ++K ++LGL PNLADFYP+L   D+QGL+ K+     
Sbjct: 189 SNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKN 248

Query: 241 RVDSVYDTFISERRRDRDRNGGVATCRDFLDSMLDFGFCDDQMRYLMAEILSASTDTTST 300
            VDS++   I ERR    + G  +   DFLD ++  GF D Q+  L+ E+LSA TDTT+T
Sbjct: 249 EVDSLWGILIKERREIHCQQGSAS--NDFLDVLIQSGFDDQQINALIIELLSAGTDTTTT 308

Query: 301 TVEWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAP 360
           TVEW M+E++K+K I++KV+EE+ +++  +N  I ES I +L YL  CVKETLRLHPPAP
Sbjct: 309 TVEWAMSEILKDKDILNKVREEM-KKVTKENRPIDESNISELQYLWRCVKETLRLHPPAP 368

Query: 361 FLLPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRF---LGSD-IEF 420
           FLLP  A   C+VMGY+IPKD+ +FVN+W IGR P+ WE+  TFNP RF    G+D ++F
Sbjct: 369 FLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDF 428

Query: 421 KGFDFRYLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIA 480
           KG+D+RYLPFG GRRICPGLPM +VQV LILATLI NF+WSLPN+ED SKLDL G++G+ 
Sbjct: 429 KGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVT 488

Query: 481 YYKEKPLSLIPKIRV 491
             K + L LIPK RV
Sbjct: 489 LQKYETLKLIPKRRV 498

[Close]
BLAST of Lag0031962 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 360.5 bits (924), Expect = 2.1e-99
Identity = 193/478 (40.38%), Postives = 277/478 (57.95%), Query Frame = 0 

Query: 18  KSRKHSPPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLISLRLGSQLAVVASTPS 77
           +S +   P PPGP   P++GN+  +G NP  +    S  +GP++SL+ GS   VV ++P 
Sbjct: 30  RSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIMSLKFGSLNTVVVTSPE 89

Query: 78  AAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWKFLRTTCRSSLFSSKAL 137
           AA E+LRT+D+ LS R    ++   +   + S++  P     W+ LR    + LFS + +
Sbjct: 90  AAREVLRTYDQILSSRTPTNSIRSINH-DKVSVVWLPPSSSRWRLLRKLSATQLFSPQRI 149

Query: 138 DSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTNVIFSKDLMEYEGDGAY 197
           ++   LR NK   LV +++  SE  EAV I    F T  N ++N++FS DL  Y+ + + 
Sbjct: 150 EATKTLRENKVKELVSFMSESSEREEAVDISRATFITALNIISNILFSVDLGNYDSNKSG 209

Query: 198 GFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRVDSVYDTFISERRRD-- 257
            F++     +E    P+ A+F+P L  LD+QG RK    CS R+  V+  FI  +  +  
Sbjct: 210 VFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSERLFKVFRGFIDAKLAEKS 269

Query: 258 -RDRNGGVATCRDFLDSMLDFGFCDD------QMRYLMAEILSASTDTTSTTVEWTMAEL 317
            RD N      RDF+D +LD    D+       + +L+ ++  A TDT S+TVEW MAEL
Sbjct: 270 LRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVHLLLDLFGAGTDTNSSTVEWAMAEL 329

Query: 318 MKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETLRLHPPAPFLLPHRALQ 377
           ++N   M K Q EI   I  Q   + ES I  L YLQA VKET RLHP AP L+P +A  
Sbjct: 330 LRNPETMVKAQAEIDCVI-GQKGVVEESDISALPYLQAVVKETFRLHPAAPLLVPRKAES 389

Query: 378 TCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSDIEFKGFDFRYLPFGSG 437
             +V+G+ +PKDT++FVN+WAIGR P  WEN S F P+RFLG DI+ +G D+   PFG+G
Sbjct: 390 DVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPERFLGKDIDLRGRDYELTPFGAG 449

Query: 438 RRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKMGIAYYKEKPLSLIP 487
           RRICPGLP+ V  V L+LA+L+ +FDW LPN      LD+    G+  +K  PL  +P
Sbjct: 450 RRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLDMDETFGLTLHKTNPLHAVP 505

[Close]
BLAST of Lag0031962 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 346.7 bits (888), Expect = 3.1e-95
Identity = 190/494 (38.46%), Postives = 287/494 (58.10%), Query Frame = 0 

Query: 4   FILLLLLLLLLKLLKSRKHS---PPLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPL 63
           F  +    L+    +SR+ S     LPPGP   P++GN+ Q+G NP  +    + ++GP+
Sbjct: 13  FCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPI 72

Query: 64  ISLRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHW 123
           +SL+ G   +VV ++P AA E+LRTHD+ LSGR    ++         S++  P     W
Sbjct: 73  MSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIR-CFGHEEVSVIWLPPSSARW 132

Query: 124 KFLRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMT 183
           + LR    + +FS +  ++  ALR  K   LV ++   SE  EAV I   ++TTV N ++
Sbjct: 133 RMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIIS 192

Query: 184 NVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRR 243
           N++FS DL  Y+   +  F++     +E    P+ A+++P +  LD+QG RK     + R
Sbjct: 193 NILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTER 252

Query: 244 VDSVYDTFISERRRDRD--RNGGVATCRDFLDSMLDFGFCDD------QMRYLMAEILSA 303
           +  V+  F+  +  ++         + RDFLDS+L     D+       + +L+ ++ +A
Sbjct: 253 LFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLLDMFTA 312

Query: 304 STDTTSTTVEWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKETL 363
            TDT+S+T+EW MAEL++N   M K Q E+ R +  QN+ + ES I  L YLQA VKET 
Sbjct: 313 GTDTSSSTLEWAMAELLRNPKTMVKAQAEMDR-VLGQNSVVQESDISGLPYLQAVVKETF 372

Query: 364 RLHPPAPFLLPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGSD 423
           RLHP AP L+P +A    +V+G+ +PKDT++ VN+WAIGR P+ WENPS F P+RF+G D
Sbjct: 373 RLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFMGKD 432

Query: 424 IEFKGFDFRYLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGKM 483
           I+ KG D+   PFG GRRICPGLP+ V  V+L+LA+L+ +FDW LPN      LD+    
Sbjct: 433 IDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETF 492

Query: 484 GIAYYKEKPLSLIP 487
           GI  ++   L  IP
Sbjct: 493 GITLHRTNTLYAIP 504

[Close]
BLAST of Lag0031962 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 345.9 bits (886), Expect = 5.4e-95
Identity = 197/495 (39.80%), Postives = 288/495 (58.18%), Query Frame = 0 

Query: 4   FILLLLLLLLLKLLKSRKHSPPL---PPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPL 63
           F  +L  LL     +SR+    L   PPGP   P++GN+  +G NP  + T  S  +GP+
Sbjct: 13  FCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGPV 72

Query: 64  ISLRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHW 123
           +SL+LG   +VV ++  A  E+L+THD+ LSGRYI +  T ++    FS+         +
Sbjct: 73  MSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISE-ATKSNNHHEFSVGWIHPSSSRF 132

Query: 124 KFLRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMT 183
           + LR    + LFS + + +  ALR  K   LV++L+   E  EAV I  V+F T  N ++
Sbjct: 133 RMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISHVSFVTALNIIS 192

Query: 184 NVIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRR 243
           N++FS +L  Y+   +  F+E      E    P+LA+F+P +  LD+QG  KK  E S R
Sbjct: 193 NILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQGNSKKMRESSGR 252

Query: 244 VDSVYDTFISER---RRDRDRNGGVATCRDFLDSMLDFGFCD------DQMRYLMAEILS 303
           +  V+  F   R   +  R     V++ +DFLD ++D    D      D++ +L+ ++  
Sbjct: 253 LLQVFREFYDARIVEKSSRSVEKDVSS-KDFLDVLIDLQQGDETEINIDEIEHLLLDMFV 312

Query: 304 ASTDTTSTTVEWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKET 363
           A TDT S+TVEW MAEL+ N   M KVQ+EI   I  QN    ES I +L YL+A VKET
Sbjct: 313 AGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVI-GQNGDFQESDISKLPYLKAVVKET 372

Query: 364 LRLHPPAPFLLPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLGS 423
            RLHP APFLL  +A    +++G+T+ KD+++ VN+WAIGR P  WENP+ F P+RFLG 
Sbjct: 373 FRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTHFEPERFLGK 432

Query: 424 DIEFKGFDFRYLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTGK 483
           +I+ KG D+   PFG+GRRICPGLP+ +  V+L+LA+L+  F+W LPN      LD+   
Sbjct: 433 EIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNGVGSEDLDMEET 492

Query: 484 MGIAYYKEKPLSLIP 487
            G+  +K  PL  +P
Sbjct: 493 FGLTVHKTNPLLAVP 504

[Close]
BLAST of Lag0031962 vs. TAIR 10
Match: AT2G45580.1 (cytochrome P450, family 76, subfamily C, polypeptide 3 )

HSP 1 Score: 343.6 bits (880), Expect = 2.7e-94
Identity = 194/503 (38.57%), Postives = 288/503 (57.26%), Query Frame = 0 

Query: 4   FILLLLLLLLLKLLKSRKHSP--PLPPGPTPWPLLGNLLQIGPNPLPAITHFSTLHGPLI 63
           + LL L        K+R+ S    LPPGP   PL+GN+ Q+G NP  ++  FS  +GP++
Sbjct: 14  YFLLTLFFFFFATAKTRRSSSTGTLPPGPPILPLVGNIFQLGFNPHRSLAAFSKTYGPIM 73

Query: 64  SLRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWK 123
           SL+LG   AVV S+P AA E LRTHD  +S R  F     A    + S++  P     W+
Sbjct: 74  SLKLGRLTAVVISSPEAAKEALRTHDHVMSAR-TFNDALRAFDHHKHSIVWIPP-SARWR 133

Query: 124 FLRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETGEAVAIRDVAFTTVFNTMTN 183
           FL+ T    L S + LD+  +LR  K   LV  +    E GEA+ +   +F T FN ++N
Sbjct: 134 FLKKTITKYLLSPQNLDAIQSLRMRKVEELVSLVNEFRERGEAIDLARASFVTSFNIISN 193

Query: 184 VIFSKDLMEYEGD-GAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRR 243
            +FS DL  Y+ +  +Y F        ++  +PN+ D++  +  LD+QG RKK+  C  +
Sbjct: 194 ALFSVDLATYDSNSSSYEFHNTVVHLTDIAGIPNVGDYFQYMRFLDLQGTRKKAVLCIEK 253

Query: 244 VDSVYDTFISERRRDR----DRNGGVATCRDFLDSMLDF------GFCDDQMRYLMAEIL 303
           +  V+  FI  R   R    ++    A+  D LDS+LD           + +++L+ ++ 
Sbjct: 254 LFRVFQEFIDARLAKRFSRTEKEPKEASSIDMLDSLLDLTQQNEAELTMNDLKHLLLDVF 313

Query: 304 SASTDTTSTTVEWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKE 363
            A TDT S+T+EW M EL ++   M K Q EI R++  QN  + ES IP L YLQA VKE
Sbjct: 314 VAGTDTNSSTMEWAMTELFRSTEKMVKAQSEI-RQVIGQNGFVQESDIPSLPYLQAIVKE 373

Query: 364 TLRLHPPAPFLLPHRALQTCQVMGYTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFLG 423
           TLRLHP AP L+P ++    Q+MG+ +PK+T++ VN+WAIGR  + WENP  F P+RFL 
Sbjct: 374 TLRLHPAAP-LIPRKSESDVQIMGFLVPKNTQVVVNVWAIGRDASVWENPMKFEPERFLL 433

Query: 424 SDIEFKGFDFRYLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLDLTG 483
            + + KG DF  +PFGSGRR+CPG+ M +  ++++LA+L+ +FDW L N   P  +D++ 
Sbjct: 434 RETDVKGRDFELIPFGSGRRMCPGISMALKTMHMVLASLLYSFDWKLQNGVVPGNIDMSE 493

Query: 484 KMGIAYYKEKPLSLIPKIRVTSS 494
             G+  +K K L  +P  + T S
Sbjct: 494 TFGLTLHKAKSLCAVPVKKPTIS 512

[Close]
BLAST of Lag0031962 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 339.7 bits (870), Expect = 3.8e-93
Identity = 176/499 (35.27%), Postives = 285/499 (57.11%), Query Frame = 0 

Query: 4   FILLLLLLLLLKLLKSRKHSPPLPPGPTPWPLLGNLLQI-GPNPLPAITHFSTLHGPLIS 63
           F  + +L+ +  L  +++    LPPGP PWP++GN+ Q+ G  P  ++T  S  HGP+++
Sbjct: 14  FTSIAVLIYVTCLFYTKRCRTRLPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMT 73

Query: 64  LRLGSQLAVVASTPSAAAEILRTHDRTLSGRYIFQTVTDAHKLRRFSLLLSPECDEHWKF 123
           LR+GS L VV S+   A EI + HD  L+GR I++ +           L++ +   +W+ 
Sbjct: 74  LRIGSMLTVVISSSEVAREIFKKHDAALAGRKIYEAMKGGKS--SDGSLITAQYGAYWRM 133

Query: 124 LRTTCRSSLFSSKALDSQAALRTNKALALVHYLASKSETG-EAVAIRDVAFTTVFNTMTN 183
           LR  C +  F ++ LD+ + +R+     ++ ++    + G + + +    F   FN + N
Sbjct: 134 LRRLCTTQFFVTRRLDAMSDVRSRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFNLIGN 193

Query: 184 VIFSKDLMEYEGDGAYGFKEHYRKTLELGLVPNLADFYPILSGLDVQGLRKKSAECSRRV 243
           ++FS+DL++ +      F  H  K +E    PN+ADF+P+L  LD QG+R+K+     + 
Sbjct: 194 LMFSRDLLDPDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKA 253

Query: 244 DSVYDTFISERRRDRDRNGGVATCRDFLDSMLDF-----------GFCDDQMRYLMAEIL 303
             +   FI ER   R+R       +D+LD +L+F            F    +  ++ E+ 
Sbjct: 254 FEIAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMF 313

Query: 304 SASTDTTSTTVEWTMAELMKNKHIMHKVQEEITREIKDQNAAIHESMIPQLHYLQACVKE 363
           +A TDTT++T+EW +AEL+ N   + K+Q E+    K  N  + E  +P L YL A + E
Sbjct: 314 TAGTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIME 373

Query: 364 TLRLHPPAPFLLPHRALQTCQVMG-YTIPKDTRIFVNIWAIGRHPTAWENPSTFNPDRFL 423
           TLRLHPP PFL+PH+A+ TC +   YTIPK+T++ VN+WAIGR P  W +P  F P+RF+
Sbjct: 374 TLRLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFI 433

Query: 424 GSD--IEFKGFDFRYLPFGSGRRICPGLPMGVVQVNLILATLIRNFDWSLPNNEDPSKLD 483
                 +FKG D+ +LPFGSGRR+CP LP+    + L + +++R+FDW+L N  +  ++D
Sbjct: 434 SDPDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMD 493

Query: 484 LTGKMGIAYYKEKPLSLIP 487
           +  ++GI   K  PL  IP
Sbjct: 494 MGERIGITLKKAVPLEAIP 510

The following BLAST results are available for this feature:
BLAST of Lag0031962 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022970204.12.7e-14955.53probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima][more]
XP_022928791.17.9e-14955.05probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata][more]
XP_023551257.15.1e-14854.83probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. ... [more]
KAG7017133.11.9e-14754.81(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma... [more]
XP_031741123.15.7e-14754.36probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus][more]
BLAST of Lag0031962 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SP065.6e-11343.74(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... [more]
Q9FXW42.8e-11243.58Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... [more]
O648997.5e-11042.62(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... [more]
O649002.9e-10942.42(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... [more]
A8CDR53.2e-10041.09Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1[more]
BLAST of Lag0031962 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1I4V71.3e-14955.53probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... [more]
A0A6J1ELA63.8e-14955.05probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
A0A6J1ER526.1e-14754.10probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
A0A1S3CT141.2e-14553.94probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656... [more]
A0A5A7UIU31.5e-14553.94Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... [more]
BLAST of Lag0031962 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G45570.12.1e-9940.38cytochrome P450, family 76, subfamily C, polypeptide 2 [more]
AT2G45550.13.1e-9538.46cytochrome P450, family 76, subfamily C, polypeptide 4 [more]
AT1G33720.15.4e-9539.80cytochrome P450, family 76, subfamily C, polypeptide 6 [more]
AT2G45580.12.7e-9438.57cytochrome P450, family 76, subfamily C, polypeptide 3 [more]
AT3G52970.13.8e-9335.27cytochrome P450, family 76, subfamily G, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 432..455
score: 28.12
coord: 386..410
score: 37.98
coord: 281..298
score: 25.3
coord: 345..363
score: 38.19
coord: 54..73
score: 32.11
coord: 174..192
score: 16.83
coord: 78..99
score: 23.61
coord: 422..432
score: 56.46
coord: 301..327
score: 29.94
IPR001128Cytochrome P450PRINTSPR00385P450coord: 346..357
score: 45.47
coord: 423..432
score: 60.56
coord: 292..309
score: 36.76
IPR001128Cytochrome P450PFAMPF00067p450coord: 27..467
e-value: 2.5E-99
score: 333.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 12..484
e-value: 6.1E-112
score: 376.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 27..489
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 6..462
e-value: 3.0E-20
score: 70.4
NoneNo IPR availablePIRSRPIRSR000209-1PIRSR000209-1coord: 28..459
e-value: 7.3E-29
score: 97.9
NoneNo IPR availablePANTHERPTHR47950CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATEDcoord: 5..489
NoneNo IPR availablePANTHERPTHR47950:SF63'-HYDROXYLASE ISOZYME, PUTATIVE-RELATEDcoord: 5..489
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 425..434

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031962.1Lag0031962.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen