Lag0031578 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0031578 vs. NCBI nr
Match: TYK11705.1 (pectinesterase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 246.1 bits (627), Expect = 2.2e-61
Identity = 128/172 (74.42%), Postives = 144/172 (83.72%), Query Frame = 0

Query: 1   MANSSSLLAFSL---LLLIVVSHAASTNDVVSTICPKTKNPPFCESVLKSAGTTDLKGLA 60
           MAN+S L+  SL   LL  ++S+ AS+NDVVSTICPKT NPPFC SVLKS GTTDLKGLA
Sbjct: 1   MANTSCLIIVSLVGVLLFTIISNVASSNDVVSTICPKTSNPPFCSSVLKSVGTTDLKGLA 60

Query: 61  TYTLNLAHTNAGKSVTLAKSLAAAATDPQLKERYSSCSENYDEVIGDIENAQKDLASGDF 120
            YTLNLAHTNA KS+TLAK LA   T+PQLK+RYSSC+E+YDE +GDIENAQKDL  GDF
Sbjct: 61  VYTLNLAHTNARKSLTLAKLLATTTTNPQLKQRYSSCAESYDEAVGDIENAQKDLTLGDF 120

Query: 121 NGVNVATSGAMTSVDDCEDDFKQPPADTSSLLKDGKTLNDICSIILVISNLL 170
           N VN+ TSGAMT +DDC+D F QPP DTS LLK+GKTLNDICSIILVISNLL
Sbjct: 121 NAVNIVTSGAMTEIDDCQDKFAQPPKDTSLLLKNGKTLNDICSIILVISNLL 172

BLAST of Lag0031578 vs. NCBI nr
Match: KAA0052848.1 (pectinesterase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 245.7 bits (626), Expect = 2.8e-61
Identity = 131/173 (75.72%), Postives = 146/173 (84.39%), Query Frame = 0

Query: 1   MANSSSLLAFS---LLLLIVVSHAASTND-VVSTICPKTKNPPFCESVLKSAGTTDLKGL 60
           MAN+S L+  S   LLL  ++S+ AS+ND VVSTICPKT NPPFC SVLKSAGTTDLKGL
Sbjct: 1   MANNSCLMIVSLIGLLLFTIISNVASSNDVVVSTICPKTSNPPFCSSVLKSAGTTDLKGL 60

Query: 61  ATYTLNLAHTNAGKSVTLAKSLAAAATDPQLKERYSSCSENYDEVIGDIENAQKDLASGD 120
           A YTLNLAHTNA KS+TLAKSLA   T+PQLK+RYSSC+E+YDE IGDIENAQKDL  GD
Sbjct: 61  AVYTLNLAHTNARKSLTLAKSLATTTTNPQLKQRYSSCAESYDEAIGDIENAQKDLTLGD 120

Query: 121 FNGVNVATSGAMTSVDDCEDDFKQPPADTSSLLKDGKTLNDICSIILVISNLL 170
           FN VN+ TSGAMT +DDC+D F QPP DTS LLK+GKTLNDICSIILVISNLL
Sbjct: 121 FNAVNIVTSGAMTEIDDCQDKFAQPPKDTSLLLKNGKTLNDICSIILVISNLL 173

BLAST of Lag0031578 vs. NCBI nr
Match: KAE8647075.1 (hypothetical protein Csa_022944 [Cucumis sativus])

HSP 1 Score: 245.4 bits (625), Expect = 3.7e-61
Identity = 126/171 (73.68%), Postives = 145/171 (84.80%), Query Frame = 0

Query: 1   MANSSSLLAFSL---LLLIVVSHAASTNDVVSTICPKTKNPPFCESVLKSAGTTDLKGLA 60
           MAN+S  +  SL   LL I++S+ AS+NDV+STICPKT NPPFC SVLKSAGTTDLKGL 
Sbjct: 296 MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV 355

Query: 61  TYTLNLAHTNAGKSVTLAKSLAAAATDPQLKERYSSCSENYDEVIGDIENAQKDLASGDF 120
            YTLNLAHTNAGKS+TLA SLA  AT+PQLK+RYSSC E+YDE +GDIENAQKDLA  DF
Sbjct: 356 VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF 415

Query: 121 NGVNVATSGAMTSVDDCEDDFKQPPADTSSLLKDGKTLNDICSIILVISNL 169
           NGVN+ TSGAMT++ DC+D F QPP DTS LL++GKT+NDICSIILVISNL
Sbjct: 416 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 466

BLAST of Lag0031578 vs. NCBI nr
Match: XP_004148234.2 (pectinesterase inhibitor [Cucumis sativus])

HSP 1 Score: 245.4 bits (625), Expect = 3.7e-61
Identity = 126/171 (73.68%), Postives = 145/171 (84.80%), Query Frame = 0

Query: 1   MANSSSLLAFSL---LLLIVVSHAASTNDVVSTICPKTKNPPFCESVLKSAGTTDLKGLA 60
           MAN+S  +  SL   LL I++S+ AS+NDV+STICPKT NPPFC SVLKSAGTTDLKGL 
Sbjct: 1   MANNSCFITVSLIGVLLFIIISYVASSNDVISTICPKTSNPPFCSSVLKSAGTTDLKGLV 60

Query: 61  TYTLNLAHTNAGKSVTLAKSLAAAATDPQLKERYSSCSENYDEVIGDIENAQKDLASGDF 120
            YTLNLAHTNAGKS+TLA SLA  AT+PQLK+RYSSC E+YDE +GDIENAQKDLA  DF
Sbjct: 61  VYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDLAVEDF 120

Query: 121 NGVNVATSGAMTSVDDCEDDFKQPPADTSSLLKDGKTLNDICSIILVISNL 169
           NGVN+ TSGAMT++ DC+D F QPP DTS LL++GKT+NDICSIILVISNL
Sbjct: 121 NGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 171

BLAST of Lag0031578 vs. NCBI nr
Match: XP_038907188.1 (pectinesterase inhibitor-like [Benincasa hispida])

HSP 1 Score: 245.4 bits (625), Expect = 3.7e-61
Identity = 129/169 (76.33%), Postives = 146/169 (86.39%), Query Frame = 0

Query: 1   MANSSSLLAFSLLLLIVVSHAASTNDVVSTICPKTKNPPFCESVLKSAGTTDLKGLATYT 60
           MANS+ +L+  L L  VVSHAAS NDVVSTICPKT NPPFC SVLKSAGTTD+K LA YT
Sbjct: 1   MANSNLILSSLLALFTVVSHAAS-NDVVSTICPKTMNPPFCASVLKSAGTTDMKRLAVYT 60

Query: 61  LNLAHTNAGKSVTLAKSLAAAATDPQLKERYSSCSENYDEVIGDIENAQKDLASGDFNGV 120
           L LAHTNA K V LAKSLAA AT+ QLK+RYSSCSE++DE +GDIE AQ+DLA+GDFN V
Sbjct: 61  LKLAHTNAAKCVALAKSLAATATNTQLKQRYSSCSESFDEAVGDIETAQEDLAAGDFNAV 120

Query: 121 NVATSGAMTSVDDCEDDFKQPPADTSSLLKDGKTLNDICSIILVISNLL 170
           N+ATSGAMT +DDC+D+FKQPP DTS LLK+G+TLNDICSIILVISNLL
Sbjct: 121 NIATSGAMTEIDDCQDNFKQPPTDTSMLLKNGRTLNDICSIILVISNLL 168

BLAST of Lag0031578 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 1.8e-26
Identity = 70/176 (39.77%), Postives = 104/176 (59.09%), Query Frame = 0

Query: 4   SSSLLAFSLLLLIVVS------HAASTNDVVSTICPKTKNPPFCESVLKS---AGTTDLK 63
           SSSL    LL+++ +S         + N ++S ICPKT+NP  C   L+S   + + DLK
Sbjct: 7   SSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLK 66

Query: 64  GLATYTLNLAHTNAGKSVTLAKSLAAAATDPQLKERYSSCSENYDEVIGDIENAQKDLAS 123
           GL  +++++A  +A ++  +  SL   ATDP+LK RY +CSENY + I  +  A++ L S
Sbjct: 67  GLGQFSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTS 126

Query: 124 GDFNGVNVATSGAMTSVDDCEDDFKQPPADTSSLLKDGKTLNDICSIILVISNLLP 171
           GD+N +N+  S A      CED F+ PP   + L +    L D+C I+LVISNLLP
Sbjct: 127 GDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLLP 182

BLAST of Lag0031578 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 6.0e-22
Identity = 63/169 (37.28%), Postives = 100/169 (59.17%), Query Frame = 0

Query: 4   SSSLLAFSLLLLIVVSHAASTNDVVSTICPKTKNPPFCESVLKSA-GTTDLKGLATYTLN 63
           +++ L+  + LL++ S  A T+  +STIC KT NP FC   L +   + +L+ LA  TL+
Sbjct: 7   NNAFLSSLMFLLLIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLD 66

Query: 64  LAHTNAGKSVTLAKSLAAAATDPQLKERYSSCSENYDEVIGDIENAQKDLASGDFNGVNV 123
                A +++   +S+     DP+ K  Y SC + Y+  IG++E A + LASGD  G+N+
Sbjct: 67  STQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNM 126

Query: 124 ATSGAMTSVDDCEDDFKQPPADTSSLLKDGKTLNDICSIILVISNLLPR 172
             S A+   D C DD K+  +  SS++ + KT+ ++C I LVISN+LPR
Sbjct: 127 KVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNMLPR 175

BLAST of Lag0031578 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 6.9e-18
Identity = 59/169 (34.91%), Postives = 89/169 (52.66%), Query Frame = 0

Query: 4   SSSLLAFSLLLLIVVSHAASTNDVVSTICPKTKNPPFCESVLKSAGTT---DLKGLATYT 63
           ++ +L  SL+  ++     +    +  IC K KN  FC S +KS   T   DL+ LA  T
Sbjct: 6   TNRVLMSSLMFFVMTGSLNAQVADIKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANIT 65

Query: 64  LNLAHTNAGKSVTLAKSLAAAATDPQLKERYSSCSENYDEVIGDIENAQKDLASGDFNGV 123
              A T+A +     +SL   AT+P +K+ Y+SC ++Y   I  + +A++ LASGD  G+
Sbjct: 66  FGSAQTSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGL 125

Query: 124 NVATSGAMTSVDDCEDDFKQPPADTSSLLKDGKTLNDICSIILVISNLL 170
           N+  S AM     CE D      D S++   G   N IC I+LVISN++
Sbjct: 126 NIKVSAAMEGPSTCEQDMADFKVDPSAVKNSGDFQN-ICGIVLVISNMM 173